AlfalfaGEDB | MS.gene60261

M. sativa cultivar XinJiangDaYe / MS.gene60261

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60261.t1	XP_004498649.1	98	100	2	0	14	113	35	134	1.00E-45	192.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60261.t1	Q8H7Y8	94.0	100	6	0	14	113	39	138	5.0e-46	184.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60261.t1	G7I6D5	98.0	100	2	0	14	113	35	134	7.2e-46	192.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene60261	0.815587	8.57E-52	-1.69E-46
MS.gene050277	MS.gene60261	0.871965	4.57E-67	-1.69E-46
MS.gene050303	MS.gene60261	0.830374	3.05E-55	-1.69E-46
MS.gene050916	MS.gene60261	0.826718	2.33E-54	-1.69E-46
MS.gene051330	MS.gene60261	0.813185	2.91E-51	-1.69E-46
MS.gene05134	MS.gene60261	0.822262	2.60E-53	-1.69E-46
MS.gene051746	MS.gene60261	0.822577	2.20E-53	-1.69E-46
MS.gene051748	MS.gene60261	0.815813	7.63E-52	-1.69E-46
MS.gene051903	MS.gene60261	0.859661	3.54E-63	-1.69E-46
MS.gene052286	MS.gene60261	0.804631	1.97E-49	-1.69E-46
MS.gene052947	MS.gene60261	0.868766	5.12E-66	-1.69E-46
MS.gene053294	MS.gene60261	0.834322	3.21E-56	-1.69E-46
MS.gene053401	MS.gene60261	0.80218	6.34E-49	-1.69E-46
MS.gene055555	MS.gene60261	0.820827	5.58E-53	-1.69E-46
MS.gene055808	MS.gene60261	0.852919	3.37E-61	-1.69E-46
MS.gene056075	MS.gene60261	0.800049	1.73E-48	-1.69E-46
MS.gene056091	MS.gene60261	0.821663	3.58E-53	-1.69E-46
MS.gene056601	MS.gene60261	0.827295	1.69E-54	-1.69E-46
MS.gene056631	MS.gene60261	0.856871	2.40E-62	-1.69E-46
MS.gene057079	MS.gene60261	0.810669	1.03E-50	-1.69E-46
MS.gene057705	MS.gene60261	0.868682	5.45E-66	-1.69E-46
MS.gene058238	MS.gene60261	0.830247	3.27E-55	-1.69E-46
MS.gene06001	MS.gene60261	0.857452	1.62E-62	-1.69E-46
MS.gene060429	MS.gene60261	0.811334	7.37E-51	-1.69E-46
MS.gene060917	MS.gene60261	0.81126	7.65E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60261.t1	MTR_1g011750	98.000	100	2	0	14	113	35	134	1.26e-66	197
MS.gene60261.t1	MTR_1g011850	98.000	100	2	0	14	113	35	134	1.26e-66	197
MS.gene60261.t1	MTR_1g071040	90.000	100	10	0	14	113	35	134	1.65e-60	181
MS.gene60261.t1	MTR_1g114050	86.000	100	14	0	14	113	32	131	2.92e-57	173
MS.gene60261.t1	MTR_4g048490	83.582	67	11	0	47	113	39	105	9.70e-35	115
MS.gene60261.t1	MTR_7g108320	60.825	97	36	2	14	109	31	126	2.10e-33	113
MS.gene60261.t1	MTR_7g114060	60.825	97	36	2	14	109	28	123	3.83e-33	112
MS.gene60261.t1	MTR_7g114040	60.825	97	36	2	14	109	28	123	3.83e-33	112
MS.gene60261.t1	MTR_2g082220	60.606	99	37	2	14	111	25	122	1.27e-32	111
MS.gene60261.t1	MTR_2g082510	60.606	99	37	2	14	111	25	122	1.34e-32	111
MS.gene60261.t1	MTR_7g093910	58.763	97	38	2	14	109	31	126	1.12e-31	109
MS.gene60261.t1	MTR_8g086640	58.416	101	40	2	14	113	33	132	4.59e-30	105
MS.gene60261.t1	MTR_4g071150	59.000	100	39	2	14	112	33	131	8.26e-30	104
MS.gene60261.t1	MTR_7g113160	54.545	99	43	2	14	111	35	132	8.99e-30	104
MS.gene60261.t1	MTR_4g064967	57.426	101	41	2	14	113	33	132	1.28e-29	103
MS.gene60261.t1	MTR_4g064005	57.426	101	41	2	14	113	33	132	1.35e-29	103
MS.gene60261.t1	MTR_2g096570	58.000	100	40	2	14	112	32	130	1.46e-29	103
MS.gene60261.t1	MTR_4g063280	57.426	101	41	2	14	113	27	126	1.58e-29	103
MS.gene60261.t1	MTR_2g096610	57.426	101	41	2	14	113	31	130	1.77e-29	103
MS.gene60261.t1	MTR_4g063410	58.763	97	38	2	14	109	33	128	8.11e-29	102
MS.gene60261.t1	MTR_7g027610	55.000	100	21	1	14	113	23	98	1.04e-25	93.2
MS.gene60261.t1	MTR_0653s0030	87.500	48	6	0	44	91	50	97	1.30e-22	86.7
MS.gene60261.t1	MTR_7g114050	51.515	99	35	4	14	111	30	116	3.52e-22	84.7
MS.gene60261.t1	MTR_4g063300	62.687	67	24	1	34	99	17	83	1.16e-19	77.8
MS.gene60261.t1	MTR_6g005150	42.000	100	47	4	14	111	35	125	1.23e-14	65.5
MS.gene60261.t1	MTR_7g427690	78.947	38	8	0	75	112	412	449	2.35e-12	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60261.t1	AT1G52740	91.000	100	9	0	14	113	35	134	9.44e-63	187
MS.gene60261.t1	AT3G54560	91.000	100	9	0	14	113	37	136	6.86e-62	185
MS.gene60261.t1	AT3G54560	91.000	100	9	0	14	113	37	136	6.86e-62	185
MS.gene60261.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene60261.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene60261.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene60261.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene60261.t1	AT4G13570	72.165	97	27	0	7	103	22	118	1.72e-44	140
MS.gene60261.t1	AT4G13570	76.667	90	21	0	14	103	35	124	5.52e-44	139
MS.gene60261.t1	AT1G08880	62.105	95	34	2	14	107	31	124	2.51e-33	113
MS.gene60261.t1	AT1G54690	61.053	95	35	2	14	107	31	124	7.88e-33	112
MS.gene60261.t1	AT4G27230	60.606	99	37	2	14	111	25	122	1.25e-32	111
MS.gene60261.t1	AT4G27230	60.606	99	37	2	14	111	25	122	1.25e-32	111
MS.gene60261.t1	AT5G54640	60.606	99	37	2	14	111	25	122	1.38e-32	111
MS.gene60261.t1	AT1G51060	60.606	99	37	2	14	111	25	122	1.88e-32	110
MS.gene60261.t1	AT3G20670	60.606	99	37	2	14	111	25	122	3.15e-32	110
MS.gene60261.t1	AT5G02560	59.406	101	39	2	14	113	33	132	4.86e-31	107
MS.gene60261.t1	AT5G59870	60.606	99	37	2	14	111	33	130	5.33e-31	107
MS.gene60261.t1	AT5G27670	60.606	99	37	2	14	111	34	131	6.10e-30	105
MS.gene60261.t1	AT5G02560	48.000	125	39	3	14	113	33	156	1.10e-25	95.1

Find 32 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGAACATTCATTCCTTTG+AGG	0.376752	1.1:-8850976	None:intergenic
CCTCTGCAGTTAAATATTCC+AGG	0.413090	1.1:-8850785	None:intergenic
CAATGGCACATGGAAGAGTT+GGG	0.442531	1.1:+8850733	MS.gene60261:CDS
AGTTTATTCTGCCGCTATCC+TGG	0.454001	1.1:+8850767	MS.gene60261:CDS
ACAATGGCACATGGAAGAGT+TGG	0.476378	1.1:+8850732	MS.gene60261:CDS
TGCTTCAGCCACATGTGTCA+TGG	0.477968	1.1:+8850414	None:intergenic
ATATTTAACTGCAGAGGTGT+TGG	0.485770	1.1:+8850791	MS.gene60261:CDS
CTCATCAAAGGAACAATAGC+TGG	0.487626	1.1:+8850909	MS.gene60261:CDS
ATAAGAGACTTGTGGATATG+AGG	0.507095	1.1:-8850945	None:intergenic
AGACGATGGATACGTCCGAC+AGG	0.539450	1.1:-8850696	None:intergenic
AACTGCAGAGGTGTTGGAGT+TGG	0.540859	1.1:+8850797	MS.gene60261:CDS
GCAGAGGTGTTGGAGTTGGC+TGG	0.558822	1.1:+8850801	MS.gene60261:CDS
CAATGCAAGCAAGGATCTGA+AGG	0.565081	1.1:+8850824	MS.gene60261:CDS
GTTGGCTGGCAATGCAAGCA+AGG	0.571379	1.1:+8850815	MS.gene60261:CDS
GAACTTGACACTCTCATCAA+AGG	0.571379	1.1:+8850897	MS.gene60261:CDS
ACTTGTTGATAAGAGACTTG+TGG	0.572936	1.1:-8850953	None:intergenic
ACATGTGTCATGGCTACAGC+AGG	0.574358	1.1:+8850424	None:intergenic
TGTCAAGTTCTTCGTCTCCA+CGG	0.583078	1.1:-8850884	None:intergenic
ATAGCAAGCTGTAGATGTCT+CGG	0.584334	1.1:-8850861	None:intergenic
TCTTATCAACAAGTCCTCAA+AGG	0.584732	1.1:+8850962	MS.gene60261:CDS
CATCTACAGCTTGCTATCCG+TGG	0.586408	1.1:+8850867	MS.gene60261:CDS
TCCATCGTCTTCTGAAGTCA+AGG	0.589423	1.1:+8850709	MS.gene60261:CDS
AAAGGAACAATAGCTGGTGG+TGG	0.608922	1.1:+8850915	MS.gene60261:CDS
CCTGGAATATTTAACTGCAG+AGG	0.615265	1.1:+8850785	MS.gene60261:CDS
AAGTCAAGGACAATGGCACA+TGG	0.622887	1.1:+8850723	MS.gene60261:CDS
GACGATGGATACGTCCGACA+GGG	0.626692	1.1:-8850695	None:intergenic
TTCTACATACAGTTCCCTGT+CGG	0.628096	1.1:+8850681	MS.gene60261:intron
AATGGCACATGGAAGAGTTG+GGG	0.645846	1.1:+8850734	MS.gene60261:CDS
TCTTCTGAAGTCAAGGACAA+TGG	0.655563	1.1:+8850716	MS.gene60261:CDS
ATCAAAGGAACAATAGCTGG+TGG	0.661257	1.1:+8850912	MS.gene60261:CDS
TCCTTGACTTCAGAAGACGA+TGG	0.668973	1.1:-8850710	None:intergenic
AGTTAAATATTCCAGGATAG+CGG	0.686777	1.1:-8850778	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTAAATTATGTTGCATTTGT+TGG	+	chr1.1:8850606-8850625	MS.gene60261:intron	25.0%
	AATAATTAACCGCTTGCAAA+AGG	-	chr1.1:8850515-8850534	None:intergenic	30.0%
	AGTTAAATATTCCAGGATAG+CGG	-	chr1.1:8850781-8850800	None:intergenic	30.0%
	ATGTAGAAAGATGTTCATGA+TGG	-	chr1.1:8850669-8850688	None:intergenic	30.0%
!	TGATTATTTCCTTTTGCAAG+CGG	+	chr1.1:8850503-8850522	MS.gene60261:intron	30.0%
!!	ATTTTAGCATGCTTAGAATG+TGG	-	chr1.1:8850573-8850592	None:intergenic	30.0%
	ATAAGAGACTTGTGGATATG+AGG	-	chr1.1:8850948-8850967	None:intergenic	35.0%
	ATTTATCCCACATCATCATC+CGG	+	chr1.1:8850639-8850658	MS.gene60261:intron	35.0%
	TCTTATCAACAAGTCCTCAA+AGG	+	chr1.1:8850962-8850981	MS.gene60261:CDS	35.0%
!	ACTTGTTGATAAGAGACTTG+TGG	-	chr1.1:8850956-8850975	None:intergenic	35.0%
!	AGATGTTCATGATGGAAAAC+CGG	-	chr1.1:8850661-8850680	None:intergenic	35.0%
!	ATATTTAACTGCAGAGGTGT+TGG	+	chr1.1:8850791-8850810	MS.gene60261:CDS	35.0%
!!	GCATGCTAAAATTTGTTGTC+TGG	+	chr1.1:8850581-8850600	MS.gene60261:intron	35.0%
	ATAGCAAGCTGTAGATGTCT+CGG	-	chr1.1:8850864-8850883	None:intergenic	40.0%
	ATCAAAGGAACAATAGCTGG+TGG	+	chr1.1:8850912-8850931	MS.gene60261:CDS	40.0%
	CCTCTGCAGTTAAATATTCC+AGG	-	chr1.1:8850788-8850807	None:intergenic	40.0%
	CCTGGAATATTTAACTGCAG+AGG	+	chr1.1:8850785-8850804	MS.gene60261:CDS	40.0%
	CTCATCAAAGGAACAATAGC+TGG	+	chr1.1:8850909-8850928	MS.gene60261:CDS	40.0%
	CTGTGAGTGACTAGTATCTT+TGG	+	chr1.1:8850469-8850488	MS.gene60261:intron	40.0%
	GAACTTGACACTCTCATCAA+AGG	+	chr1.1:8850897-8850916	MS.gene60261:CDS	40.0%
	TTCTACATACAGTTCCCTGT+CGG	+	chr1.1:8850681-8850700	MS.gene60261:intron	40.0%
!!	TCTTCTGAAGTCAAGGACAA+TGG	+	chr1.1:8850716-8850735	MS.gene60261:CDS	40.0%
	AAAGGAACAATAGCTGGTGG+TGG	+	chr1.1:8850915-8850934	MS.gene60261:CDS	45.0%
	AAGTCAAGGACAATGGCACA+TGG	+	chr1.1:8850723-8850742	MS.gene60261:CDS	45.0%
	AATGGCACATGGAAGAGTTG+GGG	+	chr1.1:8850734-8850753	MS.gene60261:CDS	45.0%
	ACAATGGCACATGGAAGAGT+TGG	+	chr1.1:8850732-8850751	MS.gene60261:CDS	45.0%
	AGTTTATTCTGCCGCTATCC+TGG	+	chr1.1:8850767-8850786	MS.gene60261:CDS	45.0%
	CAATGCAAGCAAGGATCTGA+AGG	+	chr1.1:8850824-8850843	MS.gene60261:CDS	45.0%
	CAATGGCACATGGAAGAGTT+GGG	+	chr1.1:8850733-8850752	MS.gene60261:CDS	45.0%
	TCCTTGACTTCAGAAGACGA+TGG	-	chr1.1:8850713-8850732	None:intergenic	45.0%
	TGTCAAGTTCTTCGTCTCCA+CGG	-	chr1.1:8850887-8850906	None:intergenic	45.0%
!	GGAAAACCGGATGATGATGT+GGG	-	chr1.1:8850648-8850667	None:intergenic	45.0%
!	TGGAAAACCGGATGATGATG+TGG	-	chr1.1:8850649-8850668	None:intergenic	45.0%
!!	TCCATCGTCTTCTGAAGTCA+AGG	+	chr1.1:8850709-8850728	MS.gene60261:CDS	45.0%
!!	AACTGCAGAGGTGTTGGAGT+TGG	+	chr1.1:8850797-8850816	MS.gene60261:CDS	50.0%
!!	CATCTACAGCTTGCTATCCG+TGG	+	chr1.1:8850867-8850886	MS.gene60261:CDS	50.0%
	AGACGATGGATACGTCCGAC+AGG	-	chr1.1:8850699-8850718	None:intergenic	55.0%
	GACGATGGATACGTCCGACA+GGG	-	chr1.1:8850698-8850717	None:intergenic	55.0%
	GTTGGCTGGCAATGCAAGCA+AGG	+	chr1.1:8850815-8850834	MS.gene60261:CDS	55.0%
!!	GCAGAGGTGTTGGAGTTGGC+TGG	+	chr1.1:8850801-8850820	MS.gene60261:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	8850426	8850989	8850426	ID=MS.gene60261
chr1.1	mRNA	8850426	8850989	8850426	ID=MS.gene60261.t1;Parent=MS.gene60261
chr1.1	exon	8850426	8850470	8850426	ID=MS.gene60261.t1.exon1;Parent=MS.gene60261.t1
chr1.1	CDS	8850426	8850470	8850426	ID=cds.MS.gene60261.t1;Parent=MS.gene60261.t1
chr1.1	exon	8850693	8850989	8850693	ID=MS.gene60261.t1.exon2;Parent=MS.gene60261.t1
chr1.1	CDS	8850693	8850989	8850693	ID=cds.MS.gene60261.t1;Parent=MS.gene60261.t1

Gene Sequence

>MS.gene60261

ATGTGTCATGGCTACAGCAGGTTCATCTATAACAATGATATTGCTGTGAGTGACTAGTATCTTTGGATGCTGTGTGTTGATTATTTCCTTTTGCAAGCGGTTAATTATTGTATCTCTTTGATTCTTGAAACTATTTTTTTTCTTCCACATTCTAAGCATGCTAAAATTTGTTGTCTGGCTGTAAATTATGTTGCATTTGTTGGTATTGTTTAAATTTATCCCACATCATCATCCGGTTTTCCATCATGAACATCTTTCTACATACAGTTCCCTGTCGGACGTATCCATCGTCTTCTGAAGTCAAGGACAATGGCACATGGAAGAGTTGGGGCTACAGCTGCAGTTTATTCTGCCGCTATCCTGGAATATTTAACTGCAGAGGTGTTGGAGTTGGCTGGCAATGCAAGCAAGGATCTGAAGGTGAAGCGTATTACACCGAGACATCTACAGCTTGCTATCCGTGGAGACGAAGAACTTGACACTCTCATCAAAGGAACAATAGCTGGTGGTGGTGTTATTCCTCATATCCACAAGTCTCTTATCAACAAGTCCTCAAAGGAATGA

Protein sequence

>MS.gene60261.t1

MCHGYSRFIYNNDIAFPVGRIHRLLKSRTMAHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDTLIKGTIAGGGVIPHIHKSLINKSSKE