AlfalfaGEDB | MS.gene61112

M. sativa cultivar XinJiangDaYe / MS.gene61112

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61112.t1	RHN60916.1	96.7	150	3	1	1	150	1	148	5.00E-69	270.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61112.t1	Q1S053	94.7	152	6	1	1	150	1	152	6.0e-71	268.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61112.t1	A0A396I5J8	96.7	150	3	1	1	150	1	148	3.6e-69	270.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049622	MS.gene61112	0.809395	1.93E-50	-1.69E-46
MS.gene049989	MS.gene61112	0.819283	1.26E-52	-1.69E-46
MS.gene051263	MS.gene61112	0.82039	7.03E-53	-1.69E-46
MS.gene051816	MS.gene61112	0.80956	1.78E-50	-1.69E-46
MS.gene051955	MS.gene61112	0.80791	4.01E-50	-1.69E-46
MS.gene052308	MS.gene61112	0.81191	5.53E-51	-1.69E-46
MS.gene052367	MS.gene61112	0.821238	4.49E-53	-1.69E-46
MS.gene053173	MS.gene61112	0.814622	1.40E-51	-1.69E-46
MS.gene053348	MS.gene61112	0.816301	5.93E-52	-1.69E-46
MS.gene054551	MS.gene61112	0.838926	2.15E-57	-1.69E-46
MS.gene054593	MS.gene61112	0.833182	6.18E-56	-1.69E-46
MS.gene054885	MS.gene61112	0.820624	6.22E-53	-1.69E-46
MS.gene055308	MS.gene61112	0.848893	4.59E-60	-1.69E-46
MS.gene055578	MS.gene61112	0.838977	2.09E-57	-1.69E-46
MS.gene055579	MS.gene61112	0.80066	1.30E-48	-1.69E-46
MS.gene055957	MS.gene61112	0.901561	2.37E-78	-1.69E-46
MS.gene056041	MS.gene61112	0.851631	7.83E-61	-1.69E-46
MS.gene056042	MS.gene61112	0.804853	1.77E-49	-1.69E-46
MS.gene056335	MS.gene61112	0.840689	7.48E-58	-1.69E-46
MS.gene05640	MS.gene61112	0.862102	6.43E-64	-1.69E-46
MS.gene057458	MS.gene61112	0.878818	2.06E-69	-1.69E-46
MS.gene058128	MS.gene61112	0.823578	1.28E-53	-1.69E-46
MS.gene058818	MS.gene61112	0.800167	1.64E-48	-1.69E-46
MS.gene058853	MS.gene61112	0.839614	1.43E-57	-1.69E-46
MS.gene059553	MS.gene61112	0.806549	7.80E-50	-1.69E-46
MS.gene060473	MS.gene61112	0.823081	1.68E-53	-1.69E-46
MS.gene060565	MS.gene61112	0.827811	1.27E-54	-1.69E-46
MS.gene06093	MS.gene61112	0.847716	9.70E-60	-1.69E-46
MS.gene061099	MS.gene61112	0.839047	2.00E-57	-1.69E-46
MS.gene061260	MS.gene61112	0.83994	1.17E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61112.t1	MTR_2g096610	88.667	150	15	1	1	150	1	148	8.80e-84	243
MS.gene61112.t1	MTR_4g071150	94.737	152	6	1	1	150	1	152	1.28e-83	242
MS.gene61112.t1	MTR_4g064967	95.333	150	5	1	1	150	1	148	1.76e-82	239
MS.gene61112.t1	MTR_4g064005	94.667	150	6	1	1	150	1	148	3.83e-82	238
MS.gene61112.t1	MTR_4g063280	93.939	132	7	1	20	150	14	145	6.33e-82	238
MS.gene61112.t1	MTR_4g063410	93.333	150	8	1	1	150	1	148	1.29e-81	237
MS.gene61112.t1	MTR_2g096570	89.610	154	11	2	1	150	1	153	1.86e-79	232
MS.gene61112.t1	MTR_8g086640	97.727	132	3	0	1	132	1	132	5.55e-76	223
MS.gene61112.t1	MTR_7g113160	80.451	133	25	1	10	142	13	144	1.87e-71	211
MS.gene61112.t1	MTR_7g108320	74.615	130	27	3	4	132	6	130	3.33e-60	182
MS.gene61112.t1	MTR_7g114060	73.846	130	30	1	3	132	2	127	3.98e-60	182
MS.gene61112.t1	MTR_7g114040	73.846	130	30	1	3	132	2	127	3.98e-60	182
MS.gene61112.t1	MTR_7g093910	72.441	127	32	1	4	130	5	128	2.95e-59	180
MS.gene61112.t1	MTR_2g082510	74.790	119	30	0	11	129	3	121	1.49e-58	178
MS.gene61112.t1	MTR_2g082220	73.950	119	31	0	11	129	3	121	1.19e-57	176
MS.gene61112.t1	MTR_6g005150	63.780	127	32	3	24	149	26	139	3.56e-47	149
MS.gene61112.t1	MTR_7g114050	68.333	120	23	3	17	132	10	118	9.63e-46	145
MS.gene61112.t1	MTR_4g063300	75.238	105	21	1	45	149	10	109	1.60e-45	144
MS.gene61112.t1	MTR_1g011750	59.292	113	44	2	21	132	23	134	7.86e-36	120
MS.gene61112.t1	MTR_1g011850	59.292	113	44	2	21	132	23	134	7.86e-36	120
MS.gene61112.t1	MTR_1g114050	59.633	109	42	2	21	128	20	127	7.66e-35	118
MS.gene61112.t1	MTR_1g071040	61.538	104	38	2	26	128	28	130	4.19e-34	116
MS.gene61112.t1	MTR_1g069770	50.000	96	47	1	45	139	9	104	9.29e-26	94.4
MS.gene61112.t1	MTR_7g010440	70.588	51	15	0	78	128	122	172	1.28e-16	75.1
MS.gene61112.t1	MTR_4g005910	74.419	43	11	0	87	129	4	46	2.08e-16	68.9
MS.gene61112.t1	MTR_4g048490	43.810	105	30	2	24	128	26	101	4.29e-16	69.7
MS.gene61112.t1	MTR_7g027610	41.818	110	38	3	20	128	10	94	4.30e-15	67.4
MS.gene61112.t1	MTR_2g096590	75.000	40	10	0	110	149	12	51	8.00e-13	59.7
MS.gene61112.t1	MTR_0653s0030	53.704	54	25	0	56	109	44	97	1.36e-11	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61112.t1	AT5G02560	81.699	153	25	1	1	150	1	153	1.14e-78	230
MS.gene61112.t1	AT5G02560	70.621	177	25	2	1	150	1	177	1.16e-73	218
MS.gene61112.t1	AT5G27670	75.658	152	33	3	1	150	1	150	1.43e-73	217
MS.gene61112.t1	AT5G59870	74.324	148	37	1	4	150	3	150	2.81e-71	211
MS.gene61112.t1	AT1G51060	72.093	129	36	0	11	139	3	131	1.39e-61	186
MS.gene61112.t1	AT4G27230	70.543	129	38	0	11	139	3	131	1.89e-60	183
MS.gene61112.t1	AT4G27230	70.543	129	38	0	11	139	3	131	1.89e-60	183
MS.gene61112.t1	AT3G20670	71.318	129	37	0	11	139	3	131	4.38e-60	182
MS.gene61112.t1	AT1G08880	71.318	129	34	1	7	132	2	130	1.25e-58	179
MS.gene61112.t1	AT5G54640	72.000	125	35	0	11	135	3	127	1.35e-58	178
MS.gene61112.t1	AT1G54690	72.519	131	30	3	3	132	5	130	6.06e-58	177
MS.gene61112.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.14e-36	122
MS.gene61112.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.14e-36	122
MS.gene61112.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.14e-36	122
MS.gene61112.t1	AT2G38810	59.821	112	43	2	21	131	25	135	2.14e-36	122
MS.gene61112.t1	AT1G52740	59.633	109	42	2	25	132	27	134	1.51e-34	117
MS.gene61112.t1	AT3G54560	58.716	109	43	2	25	132	29	136	2.34e-34	117
MS.gene61112.t1	AT3G54560	58.716	109	43	2	25	132	29	136	2.34e-34	117
MS.gene61112.t1	AT4G13570	47.826	92	46	2	32	122	28	118	5.87e-20	80.1
MS.gene61112.t1	AT4G13570	47.253	91	46	2	33	122	35	124	1.61e-19	79.0

Find 46 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGACTTTCCAGCCTTCTT+TGG	0.239817	4.4:-42615737	None:intergenic
GATCAGATCTAAGCCTTCTT+TGG	0.251178	4.4:-42615758	None:intergenic
CTTGTCACCGACTTCTTCCT+TGG	0.335895	4.4:-42615377	None:intergenic
TGCTGCTGAAGTTCTTGAAT+TGG	0.339223	4.4:+42615523	MS.gene61112:CDS
TTCTTTGGCTCTTTGTTAGC+AGG	0.375405	4.4:-42615722	None:intergenic
GGAGCCGGAGGAAGGAAGGG+TGG	0.384609	4.4:+42615350	MS.gene61112:CDS
AGAACTGCTGCTAGGTATAC+TGG	0.389575	4.4:-42615491	None:intergenic
CAATTTCCAGTTGGAAGAAT+CGG	0.397925	4.4:+42615419	MS.gene61112:CDS
GCCGGAGGAAGGAAGGGTGG+AGG	0.421195	4.4:+42615353	MS.gene61112:CDS
GAAGGGAGCCGGAGGAAGGA+AGG	0.422190	4.4:+42615346	MS.gene61112:CDS
TTTCAGTCTTCTTAGGCAAA+AGG	0.434052	4.4:-42615691	None:intergenic
GGCATGTGTTGTTGGCTGTT+AGG	0.442957	4.4:+42615591	MS.gene61112:CDS
AAAAGGATAGGGTTAATGTT+TGG	0.446113	4.4:-42615674	None:intergenic
CATTCCAAGGCATGTGTTGT+TGG	0.459643	4.4:+42615583	MS.gene61112:CDS
GCTGAAGTTCTTGAATTGGC+TGG	0.461152	4.4:+42615527	MS.gene61112:CDS
AACAAAGAGCCAAAGAAGGC+TGG	0.466354	4.4:+42615728	MS.gene61112:CDS
TCCTCCACCCTTCCTTCCTC+CGG	0.466462	4.4:-42615354	None:intergenic
GTCTTCTTAGGCAAAAGGAT+AGG	0.482161	4.4:-42615686	None:intergenic
TATCGACCGATTCTTCCAAC+TGG	0.484363	4.4:-42615425	None:intergenic
TACGCTCAGCGTGTTGGCAC+CGG	0.484948	4.4:+42615464	MS.gene61112:CDS
AAATTGAAGACCGGCTCTGA+TGG	0.488889	4.4:-42615402	None:intergenic
GAGCTTGGTAAATTACTTGC+TGG	0.499780	4.4:+42615623	MS.gene61112:CDS
TCTTCTTAGGCAAAAGGATA+GGG	0.501511	4.4:-42615685	None:intergenic
GAAGGAAGGGTGGAGGACCA+AGG	0.507927	4.4:+42615360	MS.gene61112:CDS
AAGCACGAAGACAACAAAGA+AGG	0.514513	4.4:+42615328	MS.gene61112:CDS
AAGGGAGCCGGAGGAAGGAA+GGG	0.514993	4.4:+42615347	MS.gene61112:CDS
ACAGCCAACAACACATGCCT+TGG	0.515073	4.4:-42615587	None:intergenic
ATCGGTCGATACTTGAAGAA+AGG	0.519021	4.4:+42615437	MS.gene61112:CDS
AGCACGAAGACAACAAAGAA+GGG	0.520726	4.4:+42615329	MS.gene61112:CDS
TTTGCCTAAGAAGACTGAAA+AGG	0.521479	4.4:+42615694	MS.gene61112:CDS
GGCTGGAAAGTCTCCAAAGA+AGG	0.525629	4.4:+42615745	MS.gene61112:CDS
TCCAACTGGAAATTGAAGAC+CGG	0.540370	4.4:-42615411	None:intergenic
TGCTAACAAAGAGCCAAAGA+AGG	0.544648	4.4:+42615724	MS.gene61112:CDS
AAGACAACAAAGAAGGGAGC+CGG	0.549880	4.4:+42615335	MS.gene61112:CDS
CAAAGAAGGGAGCCGGAGGA+AGG	0.552147	4.4:+42615342	MS.gene61112:CDS
GTTAGGAACGATGAAGAGCT+TGG	0.552797	4.4:+42615608	MS.gene61112:CDS
GCTGGTGTCACAATTGCTCA+TGG	0.556477	4.4:+42615641	MS.gene61112:CDS
GGAAGATACGCTCAGCGTGT+TGG	0.561794	4.4:+42615458	MS.gene61112:CDS
ACAACAAAGAAGGGAGCCGG+AGG	0.563100	4.4:+42615338	MS.gene61112:CDS
GCCGGTCTTCAATTTCCAGT+TGG	0.566234	4.4:+42615410	MS.gene61112:CDS
GTGACAAGATCCATCAGAGC+CGG	0.589197	4.4:+42615392	MS.gene61112:CDS
GATATTCAAGAACTGCTGCT+AGG	0.592671	4.4:-42615499	None:intergenic
TGCTAGGTATACTGGTGCAC+CGG	0.606380	4.4:-42615483	None:intergenic
AGAAGAATAGAATCATTCCA+AGG	0.617592	4.4:+42615570	MS.gene61112:CDS
TGGAGGACCAAGGAAGAAGT+CGG	0.639719	4.4:+42615370	MS.gene61112:CDS
GGTGTCACAATTGCTCATGG+TGG	0.701079	4.4:+42615644	MS.gene61112:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAAGGATAGGGTTAATGTT+TGG	-	chr4.4:42615677-42615696	None:intergenic	30.0%
	AGAAGAATAGAATCATTCCA+AGG	+	chr4.4:42615570-42615589	MS.gene61112:CDS	30.0%
	CAATTTCCAGTTGGAAGAAT+CGG	+	chr4.4:42615419-42615438	MS.gene61112:CDS	35.0%
	TCTTCTTAGGCAAAAGGATA+GGG	-	chr4.4:42615688-42615707	None:intergenic	35.0%
	TTTCAGTCTTCTTAGGCAAA+AGG	-	chr4.4:42615694-42615713	None:intergenic	35.0%
	TTTGCCTAAGAAGACTGAAA+AGG	+	chr4.4:42615694-42615713	MS.gene61112:CDS	35.0%
	AAGCACGAAGACAACAAAGA+AGG	+	chr4.4:42615328-42615347	MS.gene61112:CDS	40.0%
	AGCACGAAGACAACAAAGAA+GGG	+	chr4.4:42615329-42615348	MS.gene61112:CDS	40.0%
	ATCGGTCGATACTTGAAGAA+AGG	+	chr4.4:42615437-42615456	MS.gene61112:CDS	40.0%
	GAGCTTGGTAAATTACTTGC+TGG	+	chr4.4:42615623-42615642	MS.gene61112:CDS	40.0%
	GATATTCAAGAACTGCTGCT+AGG	-	chr4.4:42615502-42615521	None:intergenic	40.0%
	GTCTTCTTAGGCAAAAGGAT+AGG	-	chr4.4:42615689-42615708	None:intergenic	40.0%
	TCCAACTGGAAATTGAAGAC+CGG	-	chr4.4:42615414-42615433	None:intergenic	40.0%
	TGCTAACAAAGAGCCAAAGA+AGG	+	chr4.4:42615724-42615743	MS.gene61112:CDS	40.0%
	TGCTGCTGAAGTTCTTGAAT+TGG	+	chr4.4:42615523-42615542	MS.gene61112:CDS	40.0%
!!	TTCTTTGGCTCTTTGTTAGC+AGG	-	chr4.4:42615725-42615744	None:intergenic	40.0%
	AACAAAGAGCCAAAGAAGGC+TGG	+	chr4.4:42615728-42615747	MS.gene61112:CDS	45.0%
	AAGACAACAAAGAAGGGAGC+CGG	+	chr4.4:42615335-42615354	MS.gene61112:CDS	45.0%
	AGAACTGCTGCTAGGTATAC+TGG	-	chr4.4:42615494-42615513	None:intergenic	45.0%
	CATTCCAAGGCATGTGTTGT+TGG	+	chr4.4:42615583-42615602	MS.gene61112:CDS	45.0%
	GCTGAAGTTCTTGAATTGGC+TGG	+	chr4.4:42615527-42615546	MS.gene61112:CDS	45.0%
	GTTAGGAACGATGAAGAGCT+TGG	+	chr4.4:42615608-42615627	MS.gene61112:CDS	45.0%
!	AAATTGAAGACCGGCTCTGA+TGG	-	chr4.4:42615405-42615424	None:intergenic	45.0%
!	GCAGCCTTTTCAGTCTTCTT+AGG	-	chr4.4:42615701-42615720	None:intergenic	45.0%
!	TATCGACCGATTCTTCCAAC+TGG	-	chr4.4:42615428-42615447	None:intergenic	45.0%
	ACAGCCAACAACACATGCCT+TGG	-	chr4.4:42615590-42615609	None:intergenic	50.0%
	GCCGGTCTTCAATTTCCAGT+TGG	+	chr4.4:42615410-42615429	MS.gene61112:CDS	50.0%
	GGAGACTTTCCAGCCTTCTT+TGG	-	chr4.4:42615740-42615759	None:intergenic	50.0%
	GGCTGGAAAGTCTCCAAAGA+AGG	+	chr4.4:42615745-42615764	MS.gene61112:CDS	50.0%
	GTGACAAGATCCATCAGAGC+CGG	+	chr4.4:42615392-42615411	MS.gene61112:CDS	50.0%
	TGCTAGGTATACTGGTGCAC+CGG	-	chr4.4:42615486-42615505	None:intergenic	50.0%
	TGGAGGACCAAGGAAGAAGT+CGG	+	chr4.4:42615370-42615389	MS.gene61112:CDS	50.0%
!	GGCATGTGTTGTTGGCTGTT+AGG	+	chr4.4:42615591-42615610	MS.gene61112:CDS	50.0%
!	GGTGTCACAATTGCTCATGG+TGG	+	chr4.4:42615644-42615663	MS.gene61112:CDS	50.0%
!!	CTTGTCACCGACTTCTTCCT+TGG	-	chr4.4:42615380-42615399	None:intergenic	50.0%
!!	GCTGGTGTCACAATTGCTCA+TGG	+	chr4.4:42615641-42615660	MS.gene61112:CDS	50.0%
	ACAACAAAGAAGGGAGCCGG+AGG	+	chr4.4:42615338-42615357	MS.gene61112:CDS	55.0%
	GGAAGATACGCTCAGCGTGT+TGG	+	chr4.4:42615458-42615477	MS.gene61112:CDS	55.0%
	AAGGGAGCCGGAGGAAGGAA+GGG	+	chr4.4:42615347-42615366	MS.gene61112:CDS	60.0%
	CAAAGAAGGGAGCCGGAGGA+AGG	+	chr4.4:42615342-42615361	MS.gene61112:CDS	60.0%
	GAAGGAAGGGTGGAGGACCA+AGG	+	chr4.4:42615360-42615379	MS.gene61112:CDS	60.0%
	TCCTCCACCCTTCCTTCCTC+CGG	-	chr4.4:42615357-42615376	None:intergenic	60.0%
!	TACGCTCAGCGTGTTGGCAC+CGG	+	chr4.4:42615464-42615483	MS.gene61112:CDS	60.0%
	GAAGGGAGCCGGAGGAAGGA+AGG	+	chr4.4:42615346-42615365	MS.gene61112:CDS	65.0%
	GCCGGAGGAAGGAAGGGTGG+AGG	+	chr4.4:42615353-42615372	MS.gene61112:CDS	70.0%
	GGAGCCGGAGGAAGGAAGGG+TGG	+	chr4.4:42615350-42615369	MS.gene61112:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	42615320	42615772	42615320	ID=MS.gene61112
chr4.4	mRNA	42615320	42615772	42615320	ID=MS.gene61112.t1;Parent=MS.gene61112
chr4.4	exon	42615320	42615772	42615320	ID=MS.gene61112.t1.exon1;Parent=MS.gene61112.t1
chr4.4	CDS	42615320	42615772	42615320	ID=cds.MS.gene61112.t1;Parent=MS.gene61112.t1

Gene Sequence

>MS.gene61112

ATGGACGCAAGCACGAAGACAACAAAGAAGGGAGCCGGAGGAAGGAAGGGTGGAGGACCAAGGAAGAAGTCGGTGACAAGATCCATCAGAGCCGGTCTTCAATTTCCAGTTGGAAGAATCGGTCGATACTTGAAGAAAGGAAGATACGCTCAGCGTGTTGGCACCGGTGCACCAGTATACCTAGCAGCAGTTCTTGAATATCTTGCTGCTGAAGTTCTTGAATTGGCTGGAAATGCAGCACGTGATAACAAGAAGAATAGAATCATTCCAAGGCATGTGTTGTTGGCTGTTAGGAACGATGAAGAGCTTGGTAAATTACTTGCTGGTGTCACAATTGCTCATGGTGGTGTTCTTCCAAACATTAACCCTATCCTTTTGCCTAAGAAGACTGAAAAGGCTGCTCCTGCTAACAAAGAGCCAAAGAAGGCTGGAAAGTCTCCAAAGAAGGCTTAG

Protein sequence

>MS.gene61112.t1

MDASTKTTKKGAGGRKGGGPRKKSVTRSIRAGLQFPVGRIGRYLKKGRYAQRVGTGAPVYLAAVLEYLAAEVLELAGNAARDNKKNRIIPRHVLLAVRNDEELGKLLAGVTIAHGGVLPNINPILLPKKTEKAAPANKEPKKAGKSPKKA