Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63952.t1 | XP_013443601.1 | 97.1 | 69 | 2 | 0 | 23 | 91 | 365 | 433 | 2.30E-32 | 147.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63952.t1 | Q40863 | 77.3 | 44 | 10 | 0 | 23 | 66 | 375 | 418 | 3.4e-16 | 85.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63952.t1 | A0A072TKA0 | 97.1 | 69 | 2 | 0 | 23 | 91 | 365 | 433 | 1.6e-32 | 147.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene63952 | 0.811856 | 5.68E-51 | -1.69E-46 |
| MS.gene049285 | MS.gene63952 | 0.842256 | 2.89E-58 | -1.69E-46 |
| MS.gene049320 | MS.gene63952 | 0.81314 | 2.98E-51 | -1.69E-46 |
| MS.gene050163 | MS.gene63952 | 0.801897 | 7.25E-49 | -1.69E-46 |
| MS.gene050165 | MS.gene63952 | 0.813146 | 2.97E-51 | -1.69E-46 |
| MS.gene050333 | MS.gene63952 | 0.822915 | 1.83E-53 | -1.69E-46 |
| MS.gene050496 | MS.gene63952 | 0.808295 | 3.32E-50 | -1.69E-46 |
| MS.gene050504 | MS.gene63952 | 0.809053 | 2.29E-50 | -1.69E-46 |
| MS.gene050518 | MS.gene63952 | 0.802403 | 5.71E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene63952 | 0.801153 | 1.03E-48 | -1.69E-46 |
| MS.gene050876 | MS.gene63952 | 0.832404 | 9.64E-56 | -1.69E-46 |
| MS.gene051810 | MS.gene63952 | 0.809088 | 2.25E-50 | -1.69E-46 |
| MS.gene051949 | MS.gene63952 | 0.82886 | 7.11E-55 | -1.69E-46 |
| MS.gene053286 | MS.gene63952 | 0.802104 | 6.57E-49 | -1.69E-46 |
| MS.gene054600 | MS.gene63952 | 0.806745 | 7.09E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene63952 | 0.81514 | 1.08E-51 | -1.69E-46 |
| MS.gene054954 | MS.gene63952 | 0.842479 | 2.52E-58 | -1.69E-46 |
| MS.gene055362 | MS.gene63952 | 0.825924 | 3.59E-54 | -1.69E-46 |
| MS.gene055502 | MS.gene63952 | 0.801005 | 1.10E-48 | -1.69E-46 |
| MS.gene055577 | MS.gene63952 | 0.809264 | 2.06E-50 | -1.69E-46 |
| MS.gene055634 | MS.gene63952 | 0.808882 | 2.49E-50 | -1.69E-46 |
| MS.gene055638 | MS.gene63952 | 0.807862 | 4.11E-50 | -1.69E-46 |
| MS.gene055722 | MS.gene63952 | 0.801534 | 8.61E-49 | -1.69E-46 |
| MS.gene056510 | MS.gene63952 | 0.825157 | 5.46E-54 | -1.69E-46 |
| MS.gene056657 | MS.gene63952 | 0.81976 | 9.81E-53 | -1.69E-46 |
| MS.gene058419 | MS.gene63952 | 0.808037 | 3.77E-50 | -1.69E-46 |
| MS.gene058773 | MS.gene63952 | 0.848499 | 5.90E-60 | -1.69E-46 |
| MS.gene059022 | MS.gene63952 | 0.84307 | 1.76E-58 | -1.69E-46 |
| MS.gene059029 | MS.gene63952 | 0.803421 | 3.51E-49 | -1.69E-46 |
| MS.gene05913 | MS.gene63952 | 0.805279 | 1.44E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene63952 | 0.831719 | 1.42E-55 | -1.69E-46 |
| MS.gene059718 | MS.gene63952 | 0.811408 | 7.11E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene63952 | 0.813273 | 2.78E-51 | -1.69E-46 |
| MS.gene060703 | MS.gene63952 | 0.845405 | 4.14E-59 | -1.69E-46 |
| MS.gene060762 | MS.gene63952 | 0.82006 | 8.37E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63952.t1 | MTR_0004s0330 | 97.101 | 69 | 2 | 0 | 23 | 91 | 365 | 433 | 1.85e-45 | 151 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63952.t1 | AT3G50790 | 68.182 | 44 | 14 | 0 | 23 | 66 | 345 | 388 | 1.28e-18 | 79.0 |
| MS.gene63952.t1 | AT3G50790 | 68.182 | 44 | 14 | 0 | 23 | 66 | 345 | 388 | 1.28e-18 | 79.0 |
Find 32 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTCACTTGGGGTGGGTTGC+TGG | 0.187173 | 4.1:+56257080 | MS.gene63952:CDS |
| CATTGATTTCCTTCAATATC+TGG | 0.213692 | 4.1:+56257145 | MS.gene63952:CDS |
| AGCCCCTTGGACTGATCCTT+TGG | 0.318720 | 4.1:+56257121 | MS.gene63952:CDS |
| ATGATCCGATTGCTCCTTCT+AGG | 0.337718 | 4.1:+56256093 | MS.gene63952:CDS |
| GCTGCAAGTTTGTGAAGCTT+TGG | 0.364350 | 4.1:-56257180 | None:intergenic |
| GTCACTACTAACAAGCAGTT+TGG | 0.380575 | 4.1:-56257047 | None:intergenic |
| TCACAAACTTGCAGCAATCC+TGG | 0.394939 | 4.1:+56257188 | MS.gene63952:CDS |
| AATGATCGTTCTATGTTGTC+AGG | 0.437902 | 4.1:+56257019 | MS.gene63952:CDS |
| TCACGAGGAATTCCCCTAGA+AGG | 0.445703 | 4.1:-56256107 | None:intergenic |
| GACATCTAAAGGTGGTCACT+TGG | 0.451985 | 4.1:+56257067 | MS.gene63952:CDS |
| AATGTCTCTTTATTAACTCC+AGG | 0.470588 | 4.1:-56257206 | None:intergenic |
| CTAAAGGTGGTCACTTGGGG+TGG | 0.486145 | 4.1:+56257072 | MS.gene63952:CDS |
| TAAAGGTGGTCACTTGGGGT+GGG | 0.500677 | 4.1:+56257073 | MS.gene63952:CDS |
| ACATCTAAAGGTGGTCACTT+GGG | 0.501596 | 4.1:+56257068 | MS.gene63952:CDS |
| AAGCTCCACTTGGAGCCCCT+TGG | 0.524317 | 4.1:+56257108 | MS.gene63952:CDS |
| TGATCCGATTGCTCCTTCTA+GGG | 0.524323 | 4.1:+56256094 | MS.gene63952:CDS |
| CTTCTGTCTCCAGATATTGA+AGG | 0.530990 | 4.1:-56257154 | None:intergenic |
| GCTGGTGACGAAGCTCCACT+TGG | 0.538367 | 4.1:+56257098 | MS.gene63952:CDS |
| AATTCCTCGTGAAGATATCA+AGG | 0.554084 | 4.1:+56256118 | MS.gene63952:CDS |
| TTGTTAGTAGTGACATCTAA+AGG | 0.555470 | 4.1:+56257056 | MS.gene63952:CDS |
| ATTCCCCTAGAAGGAGCAAT+CGG | 0.555800 | 4.1:-56256098 | None:intergenic |
| TGTCACACTACACTTGAAGA+TGG | 0.560746 | 4.1:-56257232 | None:intergenic |
| CATCTAAAGGTGGTCACTTG+GGG | 0.569461 | 4.1:+56257069 | MS.gene63952:CDS |
| CACACTACACTTGAAGATGG+TGG | 0.585286 | 4.1:-56257229 | None:intergenic |
| ATGACCAAAGGATCAGTCCA+AGG | 0.601776 | 4.1:-56257125 | None:intergenic |
| TGACCAAAGGATCAGTCCAA+GGG | 0.614947 | 4.1:-56257124 | None:intergenic |
| GATCCGATTGCTCCTTCTAG+GGG | 0.631370 | 4.1:+56256095 | MS.gene63952:CDS |
| TGAAGGAAATCAATGACCAA+AGG | 0.632751 | 4.1:-56257137 | None:intergenic |
| TCAGTCCAAGGGGCTCCAAG+TGG | 0.656424 | 4.1:-56257113 | None:intergenic |
| GACCAAAGGATCAGTCCAAG+GGG | 0.688758 | 4.1:-56257123 | None:intergenic |
| CATACCTTGATATCTTCACG+AGG | 0.689305 | 4.1:-56256122 | None:intergenic |
| TTAGTAGTGACATCTAAAGG+TGG | 0.694576 | 4.1:+56257059 | MS.gene63952:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTTTGTTATAAAATTT+TGG | - | chr4.1:56256378-56256397 | None:intergenic | 10.0% |
| !! | TAGTTAATATAGATAGATAT+AGG | - | chr4.1:56256887-56256906 | None:intergenic | 15.0% |
| !! | TATAACAAAATTTCCATTTA+TGG | + | chr4.1:56256384-56256403 | MS.gene63952:intron | 15.0% |
| !! | AAGATGAATACTATTTATGT+TGG | + | chr4.1:56256827-56256846 | MS.gene63952:intron | 20.0% |
| !!! | TTGTTATAAAATTTTGGAGA+AGG | - | chr4.1:56256372-56256391 | None:intergenic | 20.0% |
| !!! | TTGTTGACTAAAAATGTATT+CGG | + | chr4.1:56256181-56256200 | MS.gene63952:intron | 20.0% |
| ! | AAATGTGATGGTTGAAAAAA+CGG | - | chr4.1:56256499-56256518 | None:intergenic | 25.0% |
| ! | GTCTGTGTATTAAATATCAT+TGG | - | chr4.1:56256722-56256741 | None:intergenic | 25.0% |
| ! | TTGGTTGTTAAGATATGTTT+GGG | + | chr4.1:56256608-56256627 | MS.gene63952:intron | 25.0% |
| !!! | TGATTCAGTGGATTATTTTA+TGG | + | chr4.1:56256575-56256594 | MS.gene63952:intron | 25.0% |
| AATGTCTCTTTATTAACTCC+AGG | - | chr4.1:56257209-56257228 | None:intergenic | 30.0% | |
| ATGTTAATAAAGACTAACCC+TGG | - | chr4.1:56256972-56256991 | None:intergenic | 30.0% | |
| CTTGGTTGTTAAGATATGTT+TGG | + | chr4.1:56256607-56256626 | MS.gene63952:intron | 30.0% | |
| TGGTACATATGAAAATGTGA+TGG | - | chr4.1:56256511-56256530 | None:intergenic | 30.0% | |
| TGTTAATAAAGACTAACCCT+GGG | - | chr4.1:56256971-56256990 | None:intergenic | 30.0% | |
| TTGTTAGTAGTGACATCTAA+AGG | + | chr4.1:56257056-56257075 | MS.gene63952:CDS | 30.0% | |
| ! | ATTTTATGGTATACGTCACT+TGG | + | chr4.1:56256589-56256608 | MS.gene63952:intron | 30.0% |
| ! | CGATTTCATTTGTTGATGAA+TGG | + | chr4.1:56256268-56256287 | MS.gene63952:intron | 30.0% |
| ! | TCATTTGTTGATGAATGGTA+TGG | + | chr4.1:56256273-56256292 | MS.gene63952:intron | 30.0% |
| !! | CATTGATTTCCTTCAATATC+TGG | + | chr4.1:56257145-56257164 | MS.gene63952:CDS | 30.0% |
| AAGTCACTGCAGTTATAATC+TGG | + | chr4.1:56256654-56256673 | MS.gene63952:intron | 35.0% | |
| AATGATCGTTCTATGTTGTC+AGG | + | chr4.1:56257019-56257038 | MS.gene63952:CDS | 35.0% | |
| AATTCCTCGTGAAGATATCA+AGG | + | chr4.1:56256118-56256137 | MS.gene63952:CDS | 35.0% | |
| ACATGCTTGGTATATAACAG+AGG | - | chr4.1:56256470-56256489 | None:intergenic | 35.0% | |
| AGTTATAATCTGGTAGCTGT+TGG | + | chr4.1:56256664-56256683 | MS.gene63952:intron | 35.0% | |
| CGGCATTTGAATTCCATAAA+TGG | - | chr4.1:56256400-56256419 | None:intergenic | 35.0% | |
| GTGTAAAAGAGATCGAGAAT+AGG | + | chr4.1:56256209-56256228 | MS.gene63952:intron | 35.0% | |
| GTTAATAAAGACTAACCCTG+GGG | - | chr4.1:56256970-56256989 | None:intergenic | 35.0% | |
| TGAAGGAAATCAATGACCAA+AGG | - | chr4.1:56257140-56257159 | None:intergenic | 35.0% | |
| TGTAGTTAAAATAGCGTCGT+CGG | - | chr4.1:56256420-56256439 | None:intergenic | 35.0% | |
| TTAGTAGTGACATCTAAAGG+TGG | + | chr4.1:56257059-56257078 | MS.gene63952:CDS | 35.0% | |
| ! | ATCATTGACAACTGATTCAG+TGG | + | chr4.1:56256563-56256582 | MS.gene63952:intron | 35.0% |
| ! | CATTGGCTGTTGTTTGATAT+GGG | - | chr4.1:56256705-56256724 | None:intergenic | 35.0% |
| ! | TCATTGGCTGTTGTTTGATA+TGG | - | chr4.1:56256706-56256725 | None:intergenic | 35.0% |
| AAAACGGATCACAACATGCT+TGG | - | chr4.1:56256483-56256502 | None:intergenic | 40.0% | |
| AAGATCAGACAGCTCACATT+CGG | - | chr4.1:56256746-56256765 | None:intergenic | 40.0% | |
| ACATCTAAAGGTGGTCACTT+GGG | + | chr4.1:56257068-56257087 | MS.gene63952:CDS | 40.0% | |
| ATGATTGGACCAAGGGTTAA+AGG | - | chr4.1:56256947-56256966 | None:intergenic | 40.0% | |
| ATGTACCAATGACACTCTTC+AGG | + | chr4.1:56256523-56256542 | MS.gene63952:intron | 40.0% | |
| CATACCTTGATATCTTCACG+AGG | - | chr4.1:56256125-56256144 | None:intergenic | 40.0% | |
| CTTCTGTCTCCAGATATTGA+AGG | - | chr4.1:56257157-56257176 | None:intergenic | 40.0% | |
| GTCACTACTAACAAGCAGTT+TGG | - | chr4.1:56257050-56257069 | None:intergenic | 40.0% | |
| ACCAAGGGTTAAAGGAGTTG+GGG | - | chr4.1:56256939-56256958 | None:intergenic | 45.0% | |
| ACCAATGACACTCTTCAGGT+TGG | + | chr4.1:56256527-56256546 | MS.gene63952:intron | 45.0% | |
| ATGACCAAAGGATCAGTCCA+AGG | - | chr4.1:56257128-56257147 | None:intergenic | 45.0% | |
| ATGATCCGATTGCTCCTTCT+AGG | + | chr4.1:56256093-56256112 | MS.gene63952:CDS | 45.0% | |
| ATTCCCCTAGAAGGAGCAAT+CGG | - | chr4.1:56256101-56256120 | None:intergenic | 45.0% | |
| CATCTAAAGGTGGTCACTTG+GGG | + | chr4.1:56257069-56257088 | MS.gene63952:CDS | 45.0% | |
| GACATCTAAAGGTGGTCACT+TGG | + | chr4.1:56257067-56257086 | MS.gene63952:CDS | 45.0% | |
| GACCAAGGGTTAAAGGAGTT+GGG | - | chr4.1:56256940-56256959 | None:intergenic | 45.0% | |
| GCTGCAAGTTTGTGAAGCTT+TGG | - | chr4.1:56257183-56257202 | None:intergenic | 45.0% | |
| TCACAAACTTGCAGCAATCC+TGG | + | chr4.1:56257188-56257207 | MS.gene63952:CDS | 45.0% | |
| TGACCAAAGGATCAGTCCAA+GGG | - | chr4.1:56257127-56257146 | None:intergenic | 45.0% | |
| TGATCCGATTGCTCCTTCTA+GGG | + | chr4.1:56256094-56256113 | MS.gene63952:CDS | 45.0% | |
| !! | ACCAACCTGAAGAGTGTCAT+TGG | - | chr4.1:56256531-56256550 | None:intergenic | 45.0% |
| AGACTAACCCTGGGGATGAT+TGG | - | chr4.1:56256962-56256981 | None:intergenic | 50.0% | |
| GACCAAAGGATCAGTCCAAG+GGG | - | chr4.1:56257126-56257145 | None:intergenic | 50.0% | |
| GATCCGATTGCTCCTTCTAG+GGG | + | chr4.1:56256095-56256114 | MS.gene63952:CDS | 50.0% | |
| GGACCAAGGGTTAAAGGAGT+TGG | - | chr4.1:56256941-56256960 | None:intergenic | 50.0% | |
| TAAAGGTGGTCACTTGGGGT+GGG | + | chr4.1:56257073-56257092 | MS.gene63952:CDS | 50.0% | |
| TCACGAGGAATTCCCCTAGA+AGG | - | chr4.1:56256110-56256129 | None:intergenic | 50.0% | |
| TCCCCAACTCCTTTAACCCT+TGG | + | chr4.1:56256935-56256954 | MS.gene63952:intron | 50.0% | |
| AGCCCCTTGGACTGATCCTT+TGG | + | chr4.1:56257121-56257140 | MS.gene63952:CDS | 55.0% | |
| CCTGGGGATGATTGGACCAA+GGG | - | chr4.1:56256954-56256973 | None:intergenic | 55.0% | |
| CCTTGGTCCAATCATCCCCA+GGG | + | chr4.1:56256952-56256971 | MS.gene63952:intron | 55.0% | |
| CTAAAGGTGGTCACTTGGGG+TGG | + | chr4.1:56257072-56257091 | MS.gene63952:CDS | 55.0% | |
| AAGCTCCACTTGGAGCCCCT+TGG | + | chr4.1:56257108-56257127 | MS.gene63952:CDS | 60.0% | |
| CCCTGGGGATGATTGGACCA+AGG | - | chr4.1:56256955-56256974 | None:intergenic | 60.0% | |
| CCCTTGGTCCAATCATCCCC+AGG | + | chr4.1:56256951-56256970 | MS.gene63952:intron | 60.0% | |
| TCAGTCCAAGGGGCTCCAAG+TGG | - | chr4.1:56257116-56257135 | None:intergenic | 60.0% | |
| ! | GCTGGTGACGAAGCTCCACT+TGG | + | chr4.1:56257098-56257117 | MS.gene63952:CDS | 60.0% |
| !! | GGTCACTTGGGGTGGGTTGC+TGG | + | chr4.1:56257080-56257099 | MS.gene63952:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 56256093 | 56257247 | 56256093 | ID=MS.gene63952 |
| chr4.1 | mRNA | 56256093 | 56257247 | 56256093 | ID=MS.gene63952.t1;Parent=MS.gene63952 |
| chr4.1 | exon | 56256093 | 56256139 | 56256093 | ID=MS.gene63952.t1.exon1;Parent=MS.gene63952.t1 |
| chr4.1 | CDS | 56256093 | 56256139 | 56256093 | ID=cds.MS.gene63952.t1;Parent=MS.gene63952.t1 |
| chr4.1 | exon | 56257019 | 56257247 | 56257019 | ID=MS.gene63952.t1.exon2;Parent=MS.gene63952.t1 |
| chr4.1 | CDS | 56257019 | 56257247 | 56257019 | ID=cds.MS.gene63952.t1;Parent=MS.gene63952.t1 |
| Gene Sequence |
| Protein sequence |
