AlfalfaGEDB | MS.gene64342

M. sativa cultivar XinJiangDaYe / MS.gene64342

KEGG: K11841
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64342.t1	PNX75878.1	73	63	17	0	1	63	28	90	3.30E-14	87

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene64342.t1	A0A2K3LBG3	73.0	63	17	0	1	63	28	90	2.4e-14	87.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049232	MS.gene64342	0.834428	3.01E-56	-1.69E-46
MS.gene049396	MS.gene64342	0.804736	1.87E-49	-1.69E-46
MS.gene049552	MS.gene64342	0.804474	2.12E-49	-1.69E-46
MS.gene050170	MS.gene64342	0.829332	5.46E-55	-1.69E-46
MS.gene050323	MS.gene64342	0.821716	3.48E-53	-1.69E-46
MS.gene050496	MS.gene64342	0.841456	4.70E-58	-1.69E-46
MS.gene050502	MS.gene64342	0.802489	5.48E-49	-1.69E-46
MS.gene050504	MS.gene64342	0.800635	1.31E-48	-1.69E-46
MS.gene050675	MS.gene64342	0.812908	3.35E-51	-1.69E-46
MS.gene050697	MS.gene64342	0.820589	6.33E-53	-1.69E-46
MS.gene050868	MS.gene64342	0.835652	1.48E-56	-1.69E-46
MS.gene050871	MS.gene64342	0.839369	1.65E-57	-1.69E-46
MS.gene051509	MS.gene64342	0.816165	6.37E-52	-1.69E-46
MS.gene051941	MS.gene64342	0.823355	1.45E-53	-1.69E-46
MS.gene052164	MS.gene64342	0.805717	1.17E-49	-1.69E-46
MS.gene052482	MS.gene64342	0.801441	8.99E-49	-1.69E-46
MS.gene052486	MS.gene64342	0.803256	3.80E-49	-1.69E-46
MS.gene053203	MS.gene64342	0.800239	1.58E-48	-1.69E-46
MS.gene053420	MS.gene64342	-0.808779	2.62E-50	-1.69E-46
MS.gene053574	MS.gene64342	0.808398	3.16E-50	-1.69E-46
MS.gene053626	MS.gene64342	0.811174	7.99E-51	-1.69E-46
MS.gene054595	MS.gene64342	0.81667	4.91E-52	-1.69E-46
MS.gene055502	MS.gene64342	0.800991	1.11E-48	-1.69E-46
MS.gene05589	MS.gene64342	0.816987	4.17E-52	-1.69E-46
MS.gene056166	MS.gene64342	0.811738	6.03E-51	-1.69E-46
MS.gene056387	MS.gene64342	0.813217	2.86E-51	-1.69E-46
MS.gene056504	MS.gene64342	0.834723	2.54E-56	-1.69E-46
MS.gene057019	MS.gene64342	0.801556	8.52E-49	-1.69E-46
MS.gene057076	MS.gene64342	0.805286	1.44E-49	-1.69E-46
MS.gene057086	MS.gene64342	0.818242	2.17E-52	-1.69E-46
MS.gene05787	MS.gene64342	0.814782	1.29E-51	-1.69E-46
MS.gene058157	MS.gene64342	0.819668	1.03E-52	-1.69E-46
MS.gene058171	MS.gene64342	-0.811801	5.84E-51	-1.69E-46
MS.gene058173	MS.gene64342	-0.811557	6.60E-51	-1.69E-46
MS.gene058175	MS.gene64342	-0.81123	7.77E-51	-1.69E-46
MS.gene05837	MS.gene64342	0.813549	2.42E-51	-1.69E-46
MS.gene059367	MS.gene64342	0.823452	1.37E-53	-1.69E-46
MS.gene059865	MS.gene64342	0.824639	7.24E-54	-1.69E-46
MS.gene06000	MS.gene64342	0.847688	9.87E-60	-1.69E-46
MS.gene060187	MS.gene64342	-0.80109	1.06E-48	-1.69E-46
MS.gene060593	MS.gene64342	-0.809074	2.26E-50	-1.69E-46
MS.gene060595	MS.gene64342	-0.832463	9.32E-56	-1.69E-46
MS.gene060778	MS.gene64342	0.875558	2.80E-68	-1.69E-46
MS.gene06077	MS.gene64342	0.803865	2.84E-49	-1.69E-46
MS.gene060982	MS.gene64342	0.809228	2.10E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 27 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CATAGCAGGGACACATTTCT+TGG	0.264407	5.2:+40026669	None:intergenic
GGTGGTTTGGTTGGCATAGC+AGG	0.303830	5.2:+40026655	None:intergenic
GGCACTTGCGTCTGCCTCCA+TGG	0.330551	5.2:+40026633	None:intergenic
TGCGTGCCACCAGGAACTTC+TGG	0.331803	5.2:-40026695	MS.gene64342:CDS
CACGGCCGCGCCTCTGCCAC+AGG	0.395156	5.2:-40026748	MS.gene64342:CDS
TCTTGGTTACCAGAAGTTCC+TGG	0.400394	5.2:+40026686	None:intergenic
GTCTGCCTCCATGGGTGGTT+TGG	0.427775	5.2:+40026642	None:intergenic
GCCTCCATGGGTGGTTTGGT+TGG	0.435015	5.2:+40026646	None:intergenic
TTCCACATGCCCTGTGGCAG+AGG	0.454808	5.2:+40026738	None:intergenic
GGCGCGGCCGTGATGATAAA+CGG	0.465640	5.2:+40026759	None:intergenic
CGCCTCTGCCACAGGGCATG+TGG	0.480960	5.2:-40026740	MS.gene64342:CDS
GCACTTGCGTCTGCCTCCAT+GGG	0.498020	5.2:+40026634	None:intergenic
CTTGCGTCTGCCTCCATGGG+TGG	0.501890	5.2:+40026637	None:intergenic
TGATGATTCAACTCAATTCA+AGG	0.508747	5.2:+40026611	None:intergenic
CGGTGCAACTGCGTGCCACC+AGG	0.530335	5.2:-40026704	MS.gene64342:CDS
GTGGTTTGGTTGGCATAGCA+GGG	0.546195	5.2:+40026656	None:intergenic
GCAATGGAGCATGTGCTGCT+AGG	0.562822	5.2:-40026784	MS.gene64342:CDS
ATATAAATTTCAGATTGCAA+TGG	0.566444	5.2:-40026800	None:intergenic
GATGATTCAACTCAATTCAA+GGG	0.567646	5.2:+40026612	None:intergenic
CATGCCCTGTGGCAGAGGCG+CGG	0.598879	5.2:+40026743	None:intergenic
CATGTGGAACTTGCTGCAGT+CGG	0.613537	5.2:-40026724	MS.gene64342:CDS
ACGGCCGCGCCTCTGCCACA+GGG	0.616011	5.2:-40026747	MS.gene64342:CDS
CTAGGTGCCGTTTATCATCA+CGG	0.637583	5.2:-40026766	MS.gene64342:CDS
TATGCCAACCAAACCACCCA+TGG	0.640986	5.2:-40026650	MS.gene64342:CDS
TGGTTACCAGAAGTTCCTGG+TGG	0.663688	5.2:+40026689	None:intergenic
GCCAACCAAACCACCCATGG+AGG	0.699460	5.2:-40026647	MS.gene64342:CDS
AGCAAGTTCCACATGCCCTG+TGG	0.700479	5.2:+40026732	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CATAGCAGGGACACATTTCT+TGG	+	chr5.2:40026739-40026758	None:intergenic	45.0%
	TCTTGGTTACCAGAAGTTCC+TGG	+	chr5.2:40026722-40026741	None:intergenic	45.0%
!!	CTAGGTGCCGTTTATCATCA+CGG	-	chr5.2:40026639-40026658	MS.gene64342:CDS	45.0%
	TATGCCAACCAAACCACCCA+TGG	-	chr5.2:40026755-40026774	MS.gene64342:CDS	50.0%
	TGGTTACCAGAAGTTCCTGG+TGG	+	chr5.2:40026719-40026738	None:intergenic	50.0%
!!	CATGTGGAACTTGCTGCAGT+CGG	-	chr5.2:40026681-40026700	MS.gene64342:CDS	50.0%
!!	GTGGTTTGGTTGGCATAGCA+GGG	+	chr5.2:40026752-40026771	None:intergenic	50.0%
	AGCAAGTTCCACATGCCCTG+TGG	+	chr5.2:40026676-40026695	None:intergenic	55.0%
!	GCAATGGAGCATGTGCTGCT+AGG	-	chr5.2:40026621-40026640	MS.gene64342:CDS	55.0%
!!	GGTGGTTTGGTTGGCATAGC+AGG	+	chr5.2:40026753-40026772	None:intergenic	55.0%
	GCACTTGCGTCTGCCTCCAT+GGG	+	chr5.2:40026774-40026793	None:intergenic	60.0%
	GCCAACCAAACCACCCATGG+AGG	-	chr5.2:40026758-40026777	MS.gene64342:CDS	60.0%
	GGCGCGGCCGTGATGATAAA+CGG	+	chr5.2:40026649-40026668	None:intergenic	60.0%
	GTCTGCCTCCATGGGTGGTT+TGG	+	chr5.2:40026766-40026785	None:intergenic	60.0%
	TGCGTGCCACCAGGAACTTC+TGG	-	chr5.2:40026710-40026729	MS.gene64342:CDS	60.0%
	TTCCACATGCCCTGTGGCAG+AGG	+	chr5.2:40026670-40026689	None:intergenic	60.0%
!!	GCCTCCATGGGTGGTTTGGT+TGG	+	chr5.2:40026762-40026781	None:intergenic	60.0%
	CTTGCGTCTGCCTCCATGGG+TGG	+	chr5.2:40026771-40026790	None:intergenic	65.0%
	GGCACTTGCGTCTGCCTCCA+TGG	+	chr5.2:40026775-40026794	None:intergenic	65.0%
	CATGCCCTGTGGCAGAGGCG+CGG	+	chr5.2:40026665-40026684	None:intergenic	70.0%
	CGCCTCTGCCACAGGGCATG+TGG	-	chr5.2:40026665-40026684	MS.gene64342:CDS	70.0%
	CGGTGCAACTGCGTGCCACC+AGG	-	chr5.2:40026701-40026720	MS.gene64342:CDS	70.0%
	ACGGCCGCGCCTCTGCCACA+GGG	-	chr5.2:40026658-40026677	MS.gene64342:CDS	75.0%
!	CACGGCCGCGCCTCTGCCAC+AGG	-	chr5.2:40026657-40026676	MS.gene64342:CDS	80.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	40026618	40026809	40026618	ID=MS.gene64342
chr5.2	mRNA	40026618	40026809	40026618	ID=MS.gene64342.t1;Parent=MS.gene64342
chr5.2	exon	40026618	40026809	40026618	ID=MS.gene64342.t1.exon1;Parent=MS.gene64342.t1
chr5.2	CDS	40026618	40026809	40026618	ID=cds.MS.gene64342.t1;Parent=MS.gene64342.t1

Gene Sequence	>MS.gene64342 ATTGCAATGGAGCATGTGCTGCTAGGTGCCGTTTATCATCACGGCCGCGCCTCTGCCACAGGGCATGTGGAACTTGCTGCAGTCGGTGCAACTGCGTGCCACCAGGAACTTCTGGTAACCAAGAAATGTGTCCCTGCTATGCCAACCAAACCACCCATGGAGGCAGACGCAAGTGCCCTTGAATTGAGTTGA
Protein sequence	>MS.gene64342.t1 IAMEHVLLGAVYHHGRASATGHVELAAVGATACHQELLVTKKCVPAMPTKPPMEADASALELS