AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64853


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 71 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAAATTTCTCCTACTTAA+AGG 0.151625 5.1:-14261363 MS.gene64853:CDS
TTCTGATTATATTCCATTTA+TGG 0.152965 5.1:-14262019 MS.gene64853:CDS
GACTTTGAATTTATTCCCTT+TGG 0.271822 5.1:-14261122 MS.gene64853:CDS
TCTTGTCCAAGGAAATTTAT+AGG 0.271944 5.1:+14261143 None:intergenic
ATAATTTGTAGGGTTGCTTT+TGG 0.277574 5.1:-14262129 None:intergenic
ACATTGGGACCTTGGCAATA+AGG 0.283394 5.1:+14260928 None:intergenic
TTATCAATCCAACCCATAAA+TGG 0.285094 5.1:+14262006 None:intergenic
TTCTATGGCAGTTGCATCAT+TGG 0.290490 5.1:-14261072 MS.gene64853:CDS
TCTGATTATATTCCATTTAT+GGG 0.291083 5.1:-14262018 MS.gene64853:CDS
CAACATCAGCTACATTAGTT+TGG 0.292688 5.1:-14261487 MS.gene64853:CDS
CTTGTAAATTGTTGTCCATT+TGG 0.293981 5.1:+14261898 None:intergenic
CTCTCTGGATAAAACTCTTC+TGG 0.301367 5.1:+14261179 None:intergenic
ATATTCTATATTCCATTGAT+TGG 0.324911 5.1:-14261034 MS.gene64853:CDS
AGAGATTATTGATGAACATT+TGG 0.343337 5.1:-14261923 MS.gene64853:CDS
ACATACACTATTGTCTTTGC+TGG 0.350131 5.1:+14261245 None:intergenic
TTCATAGAGACTTTGATGTT+TGG 0.352734 5.1:-14261208 MS.gene64853:CDS
GAATTTATTCCCTTTGGATC+TGG 0.366971 5.1:-14261116 MS.gene64853:CDS
AAATAAATTGCATTTACATT+GGG 0.381626 5.1:+14260913 None:intergenic
AATTTGTCCAGGTATTTCTA+TGG 0.383223 5.1:-14261087 MS.gene64853:CDS
TGATGTCTTACTCCAATTGA+AGG 0.390023 5.1:-14261860 MS.gene64853:CDS
AACATCAGCTACATTAGTTT+GGG 0.396147 5.1:-14261486 MS.gene64853:CDS
TGTACTTTCTTCATTACTGC+AGG 0.399504 5.1:+14261440 None:intergenic
CTTGGCACAAGCAATGGATT+TGG 0.400328 5.1:+14261308 None:intergenic
TCAAGGGAGATGTGCACTAT+AGG 0.409112 5.1:-14261281 MS.gene64853:CDS
ATTATATTCCATTTATGGGT+TGG 0.409477 5.1:-14262014 MS.gene64853:CDS
TATTCTATATTCCATTGATT+GGG 0.411713 5.1:-14261033 MS.gene64853:CDS
GAAAGTCCTATAAATTTCCT+TGG 0.420298 5.1:-14261149 MS.gene64853:CDS
CAAATAAATTGCATTTACAT+TGG 0.423232 5.1:+14260912 None:intergenic
AGAAAGTACAACAAGAGATT+AGG 0.442344 5.1:-14261427 MS.gene64853:CDS
TTGCATTTACATTGGGACCT+TGG 0.449956 5.1:+14260920 None:intergenic
CACATCTCCCTTGACTCCCT+TGG 0.458250 5.1:+14261290 None:intergenic
TCACATAAAAGGGATCCTTA+TGG 0.460531 5.1:-14261803 MS.gene64853:intron
GACTCCCTTGGCACAAGCAA+TGG 0.462033 5.1:+14261302 None:intergenic
GGACTTTCTAAGAATCTCTC+TGG 0.464370 5.1:+14261164 None:intergenic
TTCTTGTGTTGAGCAATCCC+TGG 0.472243 5.1:+14260963 None:intergenic
CAAACTCATGCAACATTCCA+TGG 0.474276 5.1:+14262067 None:intergenic
GATGAACATTTGGATCCAAA+TGG 0.475897 5.1:-14261913 MS.gene64853:CDS
GGGAGGAGGTATGAGGATGA+AGG 0.485420 5.1:-14262108 MS.gene64853:CDS
AAATCCATTGCTTGTGCCAA+GGG 0.486862 5.1:-14261306 MS.gene64853:CDS
GGTTGGATTGATAAACTAAG+AGG 0.489110 5.1:-14261997 MS.gene64853:CDS
ATGTGGCAATTCCCAATCAA+TGG 0.496258 5.1:+14261022 None:intergenic
TGCTTGTGCCAAGGGAGTCA+AGG 0.497929 5.1:-14261298 MS.gene64853:CDS
TCGTCTTGATAGAAATTTCA+AGG 0.500467 5.1:-14261965 MS.gene64853:CDS
CAATAGTGTATGTGAATGCT+TGG 0.505773 5.1:-14261235 MS.gene64853:CDS
TGGAATGTTGCATGAGTTTG+AGG 0.512073 5.1:-14262064 MS.gene64853:CDS
GTACTTTCTTCATTACTGCA+GGG 0.521457 5.1:+14261441 None:intergenic
CAAATCCATTGCTTGTGCCA+AGG 0.535799 5.1:-14261307 MS.gene64853:CDS
TCATCTTTGCCTTATTGCCA+AGG 0.541270 5.1:-14260937 MS.gene64853:CDS
GGAGGAGGTATGAGGATGAA+GGG 0.546637 5.1:-14262107 MS.gene64853:CDS
ATTGCCACATGGGTTGGCTA+AGG 0.549494 5.1:-14261009 MS.gene64853:CDS
GCACAAGCAATGGATTTGGT+AGG 0.557636 5.1:+14261312 None:intergenic
AACAAAGAAAGCTGCCAACA+TGG 0.561531 5.1:+14262041 None:intergenic
TTGGGAATTGCCACATGGGT+TGG 0.564911 5.1:-14261015 MS.gene64853:CDS
GTAGATAGAAGTAAATTCCA+TGG 0.568982 5.1:-14262084 MS.gene64853:CDS
GTTGGATTGATAAACTAAGA+GGG 0.569574 5.1:-14261996 MS.gene64853:CDS
GCTTGTGCCAAGGGAGTCAA+GGG 0.570906 5.1:-14261297 MS.gene64853:CDS
AATTCTTCGTCCAGATCCAA+AGG 0.585051 5.1:+14261106 None:intergenic
ATTGATTGGGAATTGCCACA+TGG 0.588027 5.1:-14261020 MS.gene64853:CDS
ACAACAATTTACAAGTGAAG+AGG 0.588981 5.1:-14261890 MS.gene64853:CDS
TAAATTCAAAGTCTTGTCCA+AGG 0.591328 5.1:+14261132 None:intergenic
AATAGTGTATGTGAATGCTT+GGG 0.599445 5.1:-14261234 MS.gene64853:CDS
GCATGAGTTTGAGGCCATGT+TGG 0.600473 5.1:-14262055 MS.gene64853:CDS
TCTGGACGAAGAATTTGTCC+AGG 0.606272 5.1:-14261098 MS.gene64853:CDS
TTGATTGGGAATTGCCACAT+GGG 0.608158 5.1:-14261019 MS.gene64853:CDS
GATAAACTAAGAGGGTTGCA+TGG 0.612774 5.1:-14261988 MS.gene64853:CDS
GCAACTGCCATAGAAATACC+TGG 0.615051 5.1:+14261080 None:intergenic
GAGGAGGTATGAGGATGAAG+GGG 0.621098 5.1:-14262106 MS.gene64853:CDS
AGGGAGATGTGCACTATAGG+TGG 0.629105 5.1:-14261278 MS.gene64853:CDS
ATTCTTCGTCCAGATCCAAA+GGG 0.634342 5.1:+14261107 None:intergenic
TGGAATATAGAATATTAGCA+AGG 0.641277 5.1:+14261042 None:intergenic
TCTTCCTTAGCCAACCCATG+TGG 0.720047 5.1:+14261005 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGAAGATAATTAAACAT+TGG - chr5.1:14261413-14261432 MS.gene64853:CDS 15.0%
!!! TTAATCTTTGTTTTAGTTTT+TGG - chr5.1:14261291-14261310 MS.gene64853:CDS 15.0%
!! AACTAAAACAAAGATTAAAG+TGG + chr5.1:14261289-14261308 None:intergenic 20.0%
!! AATTAAAATAGCTTACCATA+AGG + chr5.1:14261259-14261278 None:intergenic 20.0%
!! ATATTCTATATTCCATTGAT+TGG - chr5.1:14262010-14262029 MS.gene64853:CDS 20.0%
!! ATGAAAATATAATCCTTGTT+GGG + chr5.1:14261331-14261350 None:intergenic 20.0%
!! TCTGATTATATTCCATTTAT+GGG - chr5.1:14261026-14261045 MS.gene64853:CDS 20.0%
!! TTCTGATTATATTCCATTTA+TGG - chr5.1:14261025-14261044 MS.gene64853:CDS 20.0%
!!! TATTCTATATTCCATTGATT+GGG - chr5.1:14262011-14262030 MS.gene64853:CDS 20.0%
! ACAAAATTTCTCCTACTTAA+AGG - chr5.1:14261681-14261700 MS.gene64853:intron 25.0%
! AGAGATTATTGATGAACATT+TGG - chr5.1:14261121-14261140 MS.gene64853:CDS 25.0%
! ATTATATTCCATTTATGGGT+TGG - chr5.1:14261030-14261049 MS.gene64853:CDS 25.0%
! CATGAAAATATAATCCTTGT+TGG + chr5.1:14261332-14261351 None:intergenic 25.0%
! CATTATTATTTCCAAGTGTT+GGG + chr5.1:14261398-14261417 None:intergenic 25.0%
! CTTCAAAATATGATCAACTT+TGG + chr5.1:14261483-14261502 None:intergenic 25.0%
! TCATTATTATTTCCAAGTGT+TGG + chr5.1:14261399-14261418 None:intergenic 25.0%
! TGAAGATAATTAAACATTGG+TGG - chr5.1:14261416-14261435 MS.gene64853:CDS 25.0%
! TGGAATATAGAATATTAGCA+AGG + chr5.1:14262005-14262024 None:intergenic 25.0%
!!! AGGAAGATATTGATTTTGAA+AGG - chr5.1:14262055-14262074 MS.gene64853:CDS 25.0%
!!! ATCACTTTTGATCACATAAA+AGG - chr5.1:14261230-14261249 MS.gene64853:CDS 25.0%
!!! TCACTTTTGATCACATAAAA+GGG - chr5.1:14261231-14261250 MS.gene64853:CDS 25.0%
AATAGTGTATGTGAATGCTT+GGG - chr5.1:14261810-14261829 MS.gene64853:CDS 30.0%
ACAACAATTTACAAGTGAAG+AGG - chr5.1:14261154-14261173 MS.gene64853:CDS 30.0%
CTTGTAAATTGTTGTCCATT+TGG + chr5.1:14261149-14261168 None:intergenic 30.0%
GAAAGTCCTATAAATTTCCT+TGG - chr5.1:14261895-14261914 MS.gene64853:CDS 30.0%
GACTTTGAATTTATTCCCTT+TGG - chr5.1:14261922-14261941 MS.gene64853:CDS 30.0%
GTAGATAGAAGTAAATTCCA+TGG - chr5.1:14260960-14260979 MS.gene64853:CDS 30.0%
GTTGGATTGATAAACTAAGA+GGG - chr5.1:14261048-14261067 MS.gene64853:CDS 30.0%
TCGTCTTGATAGAAATTTCA+AGG - chr5.1:14261079-14261098 MS.gene64853:CDS 30.0%
TCTTGTCCAAGGAAATTTAT+AGG + chr5.1:14261904-14261923 None:intergenic 30.0%
TTATCAATCCAACCCATAAA+TGG + chr5.1:14261041-14261060 None:intergenic 30.0%
TTCATAGAGACTTTGATGTT+TGG - chr5.1:14261836-14261855 MS.gene64853:CDS 30.0%
! AACATCAGCTACATTAGTTT+GGG - chr5.1:14261558-14261577 MS.gene64853:intron 30.0%
! AATTTGTCCAGGTATTTCTA+TGG - chr5.1:14261957-14261976 MS.gene64853:CDS 30.0%
! AGAAAGTACAACAAGAGATT+AGG - chr5.1:14261617-14261636 MS.gene64853:intron 30.0%
! ATGAACGTTTTTCCTTCAAT+TGG + chr5.1:14261199-14261218 None:intergenic 30.0%
! TAAATTCAAAGTCTTGTCCA+AGG + chr5.1:14261915-14261934 None:intergenic 30.0%
! TTTTATGACTGCCTTTAAGT+AGG + chr5.1:14261695-14261714 None:intergenic 30.0%
!!! TACTGCAGGGTTTTTTATTA+GGG + chr5.1:14261593-14261612 None:intergenic 30.0%
!!! TTACTGCAGGGTTTTTTATT+AGG + chr5.1:14261594-14261613 None:intergenic 30.0%
ACACAAATAATCCCAACACT+TGG - chr5.1:14261384-14261403 MS.gene64853:CDS 35.0%
ACATACACTATTGTCTTTGC+TGG + chr5.1:14261802-14261821 None:intergenic 35.0%
CAACATCAGCTACATTAGTT+TGG - chr5.1:14261557-14261576 MS.gene64853:intron 35.0%
CAATAGTGTATGTGAATGCT+TGG - chr5.1:14261809-14261828 MS.gene64853:CDS 35.0%
GAATTTATTCCCTTTGGATC+TGG - chr5.1:14261928-14261947 MS.gene64853:CDS 35.0%
GATGAACATTTGGATCCAAA+TGG - chr5.1:14261131-14261150 MS.gene64853:CDS 35.0%
GGTTGGATTGATAAACTAAG+AGG - chr5.1:14261047-14261066 MS.gene64853:CDS 35.0%
GTACTTTCTTCATTACTGCA+GGG + chr5.1:14261606-14261625 None:intergenic 35.0%
TAACAGCTTAAATCCCAACA+AGG - chr5.1:14261315-14261334 MS.gene64853:CDS 35.0%
TCACATAAAAGGGATCCTTA+TGG - chr5.1:14261241-14261260 MS.gene64853:CDS 35.0%
TGATGTCTTACTCCAATTGA+AGG - chr5.1:14261184-14261203 MS.gene64853:CDS 35.0%
TGTACTTTCTTCATTACTGC+AGG + chr5.1:14261607-14261626 None:intergenic 35.0%
!! ATTGATTTTGAAAGGTTGCC+AGG - chr5.1:14262063-14262082 MS.gene64853:CDS 35.0%
!! TTGATTTTGAAAGGTTGCCA+GGG - chr5.1:14262064-14262083 MS.gene64853:CDS 35.0%
AAATCCATTGCTTGTGCCAA+GGG - chr5.1:14261738-14261757 MS.gene64853:intron 40.0%
AACAAAGAAAGCTGCCAACA+TGG + chr5.1:14261006-14261025 None:intergenic 40.0%
AATTCTTCGTCCAGATCCAA+AGG + chr5.1:14261941-14261960 None:intergenic 40.0%
ATGTGGCAATTCCCAATCAA+TGG + chr5.1:14262025-14262044 None:intergenic 40.0%
ATTCTTCGTCCAGATCCAAA+GGG + chr5.1:14261940-14261959 None:intergenic 40.0%
ATTGATTGGGAATTGCCACA+TGG - chr5.1:14262024-14262043 MS.gene64853:CDS 40.0%
CAAACTCATGCAACATTCCA+TGG + chr5.1:14260980-14260999 None:intergenic 40.0%
CTCTCTGGATAAAACTCTTC+TGG + chr5.1:14261868-14261887 None:intergenic 40.0%
GATAAACTAAGAGGGTTGCA+TGG - chr5.1:14261056-14261075 MS.gene64853:CDS 40.0%
TGGAATGTTGCATGAGTTTG+AGG - chr5.1:14260980-14260999 MS.gene64853:CDS 40.0%
TTCTATGGCAGTTGCATCAT+TGG - chr5.1:14261972-14261991 MS.gene64853:CDS 40.0%
TTGATTGGGAATTGCCACAT+GGG - chr5.1:14262025-14262044 MS.gene64853:CDS 40.0%
! GGACTTTCTAAGAATCTCTC+TGG + chr5.1:14261883-14261902 None:intergenic 40.0%
! TCATCTTTGCCTTATTGCCA+AGG - chr5.1:14262107-14262126 MS.gene64853:CDS 40.0%
ACATTGGGACCTTGGCAATA+AGG + chr5.1:14262119-14262138 None:intergenic 45.0%
ATTGCCAAGGTCCCAATGTA+AGG - chr5.1:14262120-14262139 MS.gene64853:CDS 45.0%
CAAATCCATTGCTTGTGCCA+AGG - chr5.1:14261737-14261756 MS.gene64853:intron 45.0%
GCAACTGCCATAGAAATACC+TGG + chr5.1:14261967-14261986 None:intergenic 45.0%
TCAAGGGAGATGTGCACTAT+AGG - chr5.1:14261763-14261782 MS.gene64853:intron 45.0%
TCTGGACGAAGAATTTGTCC+AGG - chr5.1:14261946-14261965 MS.gene64853:CDS 45.0%
TTCTTGTGTTGAGCAATCCC+TGG + chr5.1:14262084-14262103 None:intergenic 45.0%
! CTTGGCACAAGCAATGGATT+TGG + chr5.1:14261739-14261758 None:intergenic 45.0%
! GCACAAGCAATGGATTTGGT+AGG + chr5.1:14261735-14261754 None:intergenic 45.0%
AGGGAGATGTGCACTATAGG+TGG - chr5.1:14261766-14261785 MS.gene64853:intron 50.0%
TCTTCCTTAGCCAACCCATG+TGG + chr5.1:14262042-14262061 None:intergenic 50.0%
TTGGGAATTGCCACATGGGT+TGG - chr5.1:14262029-14262048 MS.gene64853:CDS 50.0%
! GAGGAGGTATGAGGATGAAG+GGG - chr5.1:14260938-14260957 MS.gene64853:CDS 50.0%
! GCATGAGTTTGAGGCCATGT+TGG - chr5.1:14260989-14261008 MS.gene64853:CDS 50.0%
! GGAGGAGGTATGAGGATGAA+GGG - chr5.1:14260937-14260956 MS.gene64853:CDS 50.0%
!! ATTGCCACATGGGTTGGCTA+AGG - chr5.1:14262035-14262054 MS.gene64853:CDS 50.0%
!! TGCTTTTGGGAGGAGGTATG+AGG - chr5.1:14260929-14260948 MS.gene64853:CDS 50.0%
CACATCTCCCTTGACTCCCT+TGG + chr5.1:14261757-14261776 None:intergenic 55.0%
GACTCCCTTGGCACAAGCAA+TGG + chr5.1:14261745-14261764 None:intergenic 55.0%
GCTTGTGCCAAGGGAGTCAA+GGG - chr5.1:14261747-14261766 MS.gene64853:intron 55.0%
TGCTTGTGCCAAGGGAGTCA+AGG - chr5.1:14261746-14261765 MS.gene64853:intron 55.0%
! GGGAGGAGGTATGAGGATGA+AGG - chr5.1:14260936-14260955 MS.gene64853:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 14260926 14262140 14260926 ID=MS.gene64853
chr5.1 mRNA 14260926 14262140 14260926 ID=MS.gene64853.t1;Parent=MS.gene64853
chr5.1 exon 14261804 14262140 14261804 ID=MS.gene64853.t1.exon1;Parent=MS.gene64853.t1
chr5.1 CDS 14261804 14262140 14261804 ID=cds.MS.gene64853.t1;Parent=MS.gene64853.t1
chr5.1 exon 14260926 14261534 14260926 ID=MS.gene64853.t1.exon2;Parent=MS.gene64853.t1
chr5.1 CDS 14260926 14261534 14260926 ID=cds.MS.gene64853.t1;Parent=MS.gene64853.t1
Gene Sequence

>MS.gene64853

GGTTGCTTTTGGGAGGAGGTATGAGGATGAAGGGGTAGATAGAAGTAAATTCCATGGAATGTTGCATGAGTTTGAGGCCATGTTGGCAGCTTTCTTTGTTTCTGATTATATTCCATTTATGGGTTGGATTGATAAACTAAGAGGGTTGCATGGTCGTCTTGATAGAAATTTCAAGGAGTTTGATGAATTCTATCAAGAGATTATTGATGAACATTTGGATCCAAATGGACAACAATTTACAAGTGAAGAGGTTATTGTTGATGTCTTACTCCAATTGAAGGAAAAACGTTCATTTTCTTTTGATATCACTTTTGATCACATAAAAGGGATCCTTATGGTAAGCTATTTTAATTCAATTTTCCACTTTAATCTTTGTTTTAGTTTTTGGTTAACAGCTTAAATCCCAACAAGGATTATATTTTCATGCAGCAATGACTTTATTTGTGACTAATTGAATTACACAAATAATCCCAACACTTGGAAATAATAATGAAGATAATTAAACATTGGTGGTTACTATCTTTCTTACAAACTATTAGTGATTCTATAATGTGCCAAAGTTGATCATATTTTGAAGTAGTACTTAACAACTTTTTTAACGCACAGAATATGCTTGTAGCTGCAACAGATACAACATCAGCTACATTAGTTTGGGCAATGACTGCCCTAATAAAAAACCCTGCAGTAATGAAGAAAGTACAACAAGAGATTAGGAGTTCAAGAGTTAAAAAAGACTTTTTAGATGAAGATGATATACAAAATTTCTCCTACTTAAAGGCAGTCATAAAAGAGACATTGAGACTATTCCTACCAAATCCATTGCTTGTGCCAAGGGAGTCAAGGGAGATGTGCACTATAGGTGGTTATCAAATTCCAGCAAAGACAATAGTGTATGTGAATGCTTGGGCTATTCATAGAGACTTTGATGTTTGGAAAGATCCAGAAGAGTTTTATCCAGAGAGATTCTTAGAAAGTCCTATAAATTTCCTTGGACAAGACTTTGAATTTATTCCCTTTGGATCTGGACGAAGAATTTGTCCAGGTATTTCTATGGCAGTTGCATCATTGGAACTTATCCTTGCTAATATTCTATATTCCATTGATTGGGAATTGCCACATGGGTTGGCTAAGGAAGATATTGATTTTGAAAGGTTGCCAGGGATTGCTCAACACAAGAAGAATCATCTTTGCCTTATTGCCAAGGTCCCAATGTAA

Protein sequence

>MS.gene64853.t1

GCFWEEVUGURGRUKUIPWNVAUVUGHVGSFLCFULYSIYGLDUUTKRVAWSSUUKFQGVUUILSRDYUUTFGSKWTTIYKURGYCUCLTPIEGKTFIFFUYHFUSHKRDPYEYACSCNRYNISYISLGNDCPNKKPCSNEESTTRDUEFKSUKRLFRURUYTKFLLLKGSHKRDIETIPTKSIACAKGVKGDVHYRWLSNSSKDNSVCECLGYSURLUCLERSRRVLSREILRKSYKFPWTRLUIYSLWIWTKNLSRYFYGSCIIGTYPCUYSIFHULGIATWVGUGRYUFUKVARDCSTQEESSLPYCQGPNV