AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66338

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66338.t1 MTR_6g091800 96.285 323 4 1 1 323 1 315 0.0 630
MS.gene66338.t1 MTR_6g091800 89.625 347 4 2 1 323 1 339 0.0 619
MS.gene66338.t1 MTR_6g091800 88.750 320 4 2 1 296 1 312 0.0 563
MS.gene66338.t1 MTR_6g034145 97.701 261 6 0 63 323 1 261 0.0 528
MS.gene66338.t1 MTR_6g034145 25.854 205 127 4 26 217 60 252 2.32e-12 66.6
MS.gene66338.t1 MTR_6g091800 83.591 323 4 2 1 323 1 274 0.0 527
MS.gene66338.t1 MTR_6g091800 77.810 347 4 3 1 323 1 298 0.0 516
MS.gene66338.t1 MTR_6g091800 95.294 255 4 1 1 255 1 247 1.34e-177 492
MS.gene66338.t1 MTR_6g091800 27.404 208 124 5 122 317 18 210 1.70e-13 69.7
MS.gene66338.t1 MTR_6g092210 91.188 261 17 1 63 323 1 255 2.84e-176 488
MS.gene66338.t1 MTR_6g092210 26.633 199 127 4 26 217 60 246 7.57e-13 67.8
MS.gene66338.t1 MTR_6g091800 87.097 279 4 2 1 255 1 271 1.27e-172 480
MS.gene66338.t1 MTR_6g091910 88.119 202 22 1 122 323 18 217 3.42e-129 367
MS.gene66338.t1 MTR_6g091910 37.118 229 111 5 1 217 1 208 1.42e-33 123
MS.gene66338.t1 MTR_6g091800 94.792 192 2 1 1 192 1 184 7.61e-129 365
MS.gene66338.t1 MTR_3g104600 53.988 326 143 3 1 319 9 334 1.49e-124 360
MS.gene66338.t1 MTR_6g091800 84.259 216 2 2 1 192 1 208 7.42e-124 353
MS.gene66338.t1 MTR_5g009500 36.550 342 179 8 4 319 14 343 3.93e-60 196
MS.gene66338.t1 MTR_8g027975 33.628 339 181 7 13 319 32 358 1.43e-56 187
MS.gene66338.t1 MTR_7g064980 34.641 306 168 6 26 314 24 314 6.47e-50 169
MS.gene66338.t1 MTR_3g098460 33.108 296 176 6 24 314 202 480 1.39e-47 167
MS.gene66338.t1 MTR_7g084400 33.220 295 168 5 27 314 213 485 7.22e-46 162
MS.gene66338.t1 MTR_8g096050 32.026 306 185 6 14 314 215 502 1.25e-44 159
MS.gene66338.t1 MTR_8g096050 33.668 199 119 4 24 220 318 505 8.42e-26 107
MS.gene66338.t1 MTR_5g081150 33.663 303 173 5 26 314 33 321 4.55e-43 151
MS.gene66338.t1 MTR_5g081150 28.070 228 139 7 105 321 16 229 4.95e-13 69.3
MS.gene66338.t1 MTR_5g081150 31.472 197 119 5 26 219 140 323 6.27e-12 65.9
MS.gene66338.t1 MTR_1g012030 31.525 295 176 6 25 314 137 410 7.61e-41 147
MS.gene66338.t1 MTR_1g012030 29.592 196 124 3 20 215 227 408 8.51e-21 92.4
MS.gene66338.t1 MTR_1g012030 26.415 212 128 6 117 322 132 321 9.60e-13 68.9
MS.gene66338.t1 MTR_3g107510 29.831 295 181 6 25 314 120 393 7.09e-39 142
MS.gene66338.t1 MTR_3g107510 24.878 205 127 6 123 322 122 304 2.47e-12 67.4
MS.gene66338.t1 MTR_3g104500 29.487 312 184 10 23 316 30 323 9.10e-38 137
MS.gene66338.t1 MTR_3g104500 24.638 207 144 5 122 322 33 233 6.81e-14 71.6
MS.gene66338.t1 MTR_7g068340 29.825 285 160 5 38 311 98 353 2.89e-36 134
MS.gene66338.t1 MTR_4g114320 27.609 297 185 6 25 314 121 394 7.08e-36 134
MS.gene66338.t1 MTR_4g114320 29.353 201 127 4 20 220 212 397 3.49e-19 87.8
MS.gene66338.t1 MTR_4g119770 27.217 327 182 7 29 315 16 326 9.64e-35 129
MS.gene66338.t1 MTR_4g119770 27.451 204 131 5 121 313 12 209 1.26e-17 82.4
MS.gene66338.t1 MTR_2g436930 26.168 321 189 5 29 314 17 324 1.16e-34 129
MS.gene66338.t1 MTR_2g436930 28.972 214 137 5 109 313 1 208 5.17e-21 92.0
MS.gene66338.t1 MTR_8g090210 29.674 337 190 8 7 318 87 401 1.81e-31 122
MS.gene66338.t1 MTR_5g081150 34.348 230 123 5 26 241 33 248 3.59e-30 115
MS.gene66338.t1 MTR_5g081150 28.070 228 139 7 105 321 16 229 1.44e-13 70.1
MS.gene66338.t1 MTR_5g081150 34.348 230 123 5 26 241 33 248 3.74e-30 115
MS.gene66338.t1 MTR_5g081150 28.070 228 139 7 105 321 16 229 1.47e-13 69.7
MS.gene66338.t1 MTR_4g016730 29.371 286 157 7 38 311 100 352 2.12e-29 115
MS.gene66338.t1 MTR_7g109135 28.956 297 186 6 29 314 24 306 3.70e-29 114
MS.gene66338.t1 MTR_7g109135 31.138 167 97 6 66 226 165 319 1.12e-11 65.1
MS.gene66338.t1 MTR_4g078545 29.431 299 190 6 23 311 71 358 1.10e-28 114
MS.gene66338.t1 MTR_3g074490 30.159 315 176 7 24 316 113 405 1.14e-28 114
MS.gene66338.t1 MTR_4g119770 28.077 260 142 5 29 257 16 261 1.43e-27 108
MS.gene66338.t1 MTR_4g119770 27.451 204 131 5 121 313 12 209 6.44e-18 82.4
MS.gene66338.t1 MTR_4g005330 25.714 315 188 10 25 316 64 355 1.54e-27 110
MS.gene66338.t1 MTR_3g028590 28.428 299 193 6 23 311 93 380 2.66e-27 110
MS.gene66338.t1 MTR_3g028590 28.428 299 193 6 23 311 89 376 2.69e-27 110
MS.gene66338.t1 MTR_2g098070 28.094 299 194 6 23 311 62 349 3.13e-27 109
MS.gene66338.t1 MTR_7g084400 33.333 222 118 6 27 237 207 409 5.20e-27 110
MS.gene66338.t1 MTR_7g084400 26.020 196 120 5 123 312 207 383 9.12e-15 75.1
MS.gene66338.t1 MTR_4g005330 25.641 312 186 10 28 316 1 289 9.37e-27 107
MS.gene66338.t1 MTR_8g036880 28.094 299 195 6 23 311 98 386 8.05e-26 106
MS.gene66338.t1 MTR_7g109135 27.946 297 182 6 29 314 24 299 2.13e-25 103
MS.gene66338.t1 MTR_1g087840 28.527 319 184 11 19 314 6 303 5.58e-25 102
MS.gene66338.t1 MTR_1g087840 29.630 216 136 8 113 323 8 212 2.36e-13 70.1
MS.gene66338.t1 MTR_8g107270 26.367 311 197 10 25 321 16 308 1.50e-24 101
MS.gene66338.t1 MTR_2g436930 28.972 214 121 3 119 314 34 234 2.47e-24 99.4
MS.gene66338.t1 MTR_2g436930 24.123 228 156 5 7 226 20 238 3.73e-14 71.2
MS.gene66338.t1 MTR_5g055410 27.719 285 185 6 39 314 33 305 4.38e-24 100
MS.gene66338.t1 MTR_8g098240 25.175 286 192 6 28 312 321 585 1.78e-20 92.4
MS.gene66338.t1 MTR_7g109135 27.801 241 154 5 80 314 2 228 1.92e-20 89.0
MS.gene66338.t1 MTR_7g109135 31.138 167 97 6 66 226 87 241 1.25e-11 63.9
MS.gene66338.t1 MTR_5g030530 25.581 301 205 8 24 314 5 296 1.84e-19 87.4
MS.gene66338.t1 MTR_8g066975 25.253 297 204 7 28 314 8 296 7.42e-19 85.5
MS.gene66338.t1 MTR_8g076760 24.832 298 190 8 43 321 30 312 4.75e-18 83.6
MS.gene66338.t1 MTR_3g025800 24.576 354 224 10 1 323 1 342 1.03e-17 83.2
MS.gene66338.t1 MTR_7g109135 30.108 186 110 5 135 314 36 207 1.69e-17 80.5
MS.gene66338.t1 MTR_7g109135 31.138 167 97 6 66 226 66 220 5.01e-12 64.7
MS.gene66338.t1 MTR_4g012850 30.303 198 116 6 21 211 104 286 3.66e-17 80.9
MS.gene66338.t1 MTR_4g012850 26.207 290 174 9 17 289 1 267 1.29e-16 79.3
MS.gene66338.t1 MTR_2g102010 31.551 187 110 3 40 220 123 297 1.44e-16 79.0
MS.gene66338.t1 MTR_5g073040 25.000 300 191 7 27 315 39 315 5.08e-16 77.8
MS.gene66338.t1 MTR_7g015870 28.708 209 119 5 28 220 123 317 2.70e-15 75.9
MS.gene66338.t1 MTR_8g092080 31.905 210 122 7 13 219 116 307 4.05e-15 75.1
MS.gene66338.t1 MTR_8g092080 21.384 318 202 7 4 314 29 305 1.34e-11 64.7
MS.gene66338.t1 MTR_8g092080 27.143 210 107 7 123 323 48 220 1.91e-11 64.3
MS.gene66338.t1 MTR_6g034515 25.888 197 135 3 28 223 161 347 1.33e-14 73.9
MS.gene66338.t1 MTR_6g034515 24.497 298 183 11 43 323 83 355 7.18e-12 65.9
MS.gene66338.t1 MTR_7g117030 25.769 260 164 11 69 311 85 332 2.38e-14 73.2
MS.gene66338.t1 MTR_7g015870 27.941 204 117 5 28 215 119 308 2.82e-13 70.1
MS.gene66338.t1 MTR_1g097890 28.713 202 120 6 28 223 132 315 6.26e-13 68.9
MS.gene66338.t1 MTR_1g097890 22.500 320 204 8 7 320 31 312 1.91e-12 67.4
MS.gene66338.t1 MTR_5g081910 24.161 298 200 8 43 315 24 320 1.70e-12 67.8
MS.gene66338.t1 MTR_1g090330 23.039 204 135 4 21 215 105 295 5.11e-12 65.9
MS.gene66338.t1 MTR_5g081910 25.481 208 140 5 122 315 40 246 6.01e-12 65.5
MS.gene66338.t1 MTR_3g100570 24.000 200 130 5 25 215 122 308 1.38e-11 64.7
MS.gene66338.t1 MTR_4g088545 26.601 203 135 4 25 222 10 203 3.16e-11 62.8
MS.gene66338.t1 MTR_4g088545 26.768 198 131 4 25 217 10 198 3.18e-11 63.2
MS.gene66338.t1 MTR_4g088545 26.768 198 131 4 25 217 10 198 3.58e-11 63.5
MS.gene66338.t1 MTR_1g088995 22.481 258 176 5 5 256 11 250 5.84e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66338.t1 AT4G26180 67.937 315 100 1 8 321 2 316 2.46e-161 453
MS.gene66338.t1 AT4G26180 67.241 290 94 1 8 296 2 291 3.11e-146 414
MS.gene66338.t1 AT1G14560 56.269 327 135 3 1 320 1 326 2.46e-123 357
MS.gene66338.t1 AT1G14560 55.776 303 126 3 1 296 1 302 2.87e-109 320
MS.gene66338.t1 AT1G14560 57.205 229 90 3 99 320 1 228 1.23e-87 263
MS.gene66338.t1 AT1G14560 29.864 221 128 7 13 214 7 219 7.74e-17 79.0
MS.gene66338.t1 AT4G01100 35.913 323 170 6 23 319 38 349 7.04e-60 196
MS.gene66338.t1 AT4G01100 34.718 337 169 7 23 319 38 363 1.73e-57 190
MS.gene66338.t1 AT4G01100 34.839 310 165 6 36 319 1 299 4.56e-54 179
MS.gene66338.t1 AT3G55640 35.152 330 179 8 1 315 17 326 2.00e-53 178
MS.gene66338.t1 AT3G55640 35.152 330 179 8 1 315 17 326 2.00e-53 178
MS.gene66338.t1 AT2G37890 36.066 305 165 6 25 314 43 332 4.42e-51 172
MS.gene66338.t1 AT3G53940 34.839 310 171 7 25 318 71 365 6.28e-51 172
MS.gene66338.t1 AT5G61810 33.784 296 166 7 24 314 62 332 3.96e-49 167
MS.gene66338.t1 AT5G61810 32.653 196 114 3 27 220 156 335 9.44e-22 94.4
MS.gene66338.t1 AT5G61810 25.000 196 122 5 123 315 65 238 6.56e-11 62.8
MS.gene66338.t1 AT5G61810 33.784 296 166 7 24 314 205 475 1.03e-47 167
MS.gene66338.t1 AT5G61810 32.653 196 114 3 27 220 299 478 1.86e-21 95.1
MS.gene66338.t1 AT5G51050 32.903 310 178 9 14 316 211 497 1.27e-45 162
MS.gene66338.t1 AT5G51050 31.500 200 112 6 27 220 318 498 2.49e-18 85.9
MS.gene66338.t1 AT5G07320 31.206 282 166 6 37 314 219 476 4.45e-41 149
MS.gene66338.t1 AT5G07320 30.151 199 115 4 27 220 300 479 4.89e-19 87.8
MS.gene66338.t1 AT5G07320 25.556 180 114 4 137 315 223 383 7.55e-11 63.2
MS.gene66338.t1 AT5G56450 31.629 313 176 7 23 314 30 325 9.66e-41 145
MS.gene66338.t1 AT4G32400 33.221 298 167 7 25 314 112 385 8.33e-40 144
MS.gene66338.t1 AT4G32400 28.155 206 132 5 20 224 202 392 1.23e-16 80.5
MS.gene66338.t1 AT4G32400 26.108 203 124 4 123 321 114 294 7.34e-13 69.3
MS.gene66338.t1 AT3G51870 29.371 286 160 5 38 311 102 357 1.51e-37 138
MS.gene66338.t1 AT3G55640 35.938 256 129 8 1 241 17 252 1.74e-37 134
MS.gene66338.t1 AT3G55640 28.780 205 121 6 122 315 37 227 2.04e-16 78.2
MS.gene66338.t1 AT3G55640 35.938 256 129 8 1 241 17 252 1.74e-37 134
MS.gene66338.t1 AT3G55640 28.780 205 121 6 122 315 37 227 2.04e-16 78.2
MS.gene66338.t1 AT3G53940 36.207 232 118 6 25 241 71 287 7.73e-37 134
MS.gene66338.t1 AT3G53940 28.502 207 123 6 118 313 68 260 4.29e-13 68.9
MS.gene66338.t1 AT5G48970 27.469 324 184 6 29 314 24 334 1.64e-35 131
MS.gene66338.t1 AT5G48970 29.208 202 130 5 121 314 20 216 5.33e-20 89.4
MS.gene66338.t1 AT3G21390 27.554 323 184 5 29 314 21 330 3.16e-34 128
MS.gene66338.t1 AT3G21390 28.436 211 132 6 113 313 9 210 1.27e-18 85.5
MS.gene66338.t1 AT3G21390 27.469 324 185 5 29 315 43 353 3.25e-34 128
MS.gene66338.t1 AT3G21390 28.436 211 132 6 113 313 31 232 1.59e-18 85.5
MS.gene66338.t1 AT3G21390 23.350 197 135 3 28 211 153 346 9.68e-11 62.4
MS.gene66338.t1 AT5G01500 29.452 292 158 6 38 314 130 388 4.88e-34 129
MS.gene66338.t1 AT3G20240 26.667 315 183 9 24 314 49 339 3.03e-31 120
MS.gene66338.t1 AT5G48970 27.106 273 162 4 66 314 4 263 3.29e-30 115
MS.gene66338.t1 AT4G28390 29.487 312 193 7 16 311 66 366 4.03e-30 118
MS.gene66338.t1 AT4G28390 29.487 312 193 7 16 311 66 366 4.03e-30 118
MS.gene66338.t1 AT5G13490 29.766 299 190 6 23 311 84 372 9.99e-29 114
MS.gene66338.t1 AT5G13490 29.766 299 190 6 23 311 84 372 9.99e-29 114
MS.gene66338.t1 AT5G64970 29.747 316 178 6 25 318 133 426 1.23e-28 114
MS.gene66338.t1 AT5G64970 29.393 313 177 6 28 318 12 302 3.08e-28 111
MS.gene66338.t1 AT3G08580 29.431 299 191 6 23 311 80 368 1.71e-27 110
MS.gene66338.t1 AT3G08580 29.431 299 191 6 23 311 80 368 1.71e-27 110
MS.gene66338.t1 AT1G78180 31.761 318 165 9 25 316 126 417 3.99e-27 110
MS.gene66338.t1 AT1G25380 26.644 289 186 8 39 315 33 307 2.20e-24 102
MS.gene66338.t1 AT1G25380 26.644 289 186 8 39 315 33 307 2.20e-24 102
MS.gene66338.t1 AT5G17400 27.211 294 186 6 23 302 10 289 2.82e-24 100
MS.gene66338.t1 AT4G11440 28.808 302 183 8 23 314 324 603 4.74e-24 103
MS.gene66338.t1 AT4G11440 28.808 302 183 8 23 314 324 603 4.74e-24 103
MS.gene66338.t1 AT2G47490 27.119 295 183 8 39 318 29 306 2.21e-22 95.9
MS.gene66338.t1 AT2G47490 30.208 192 118 6 136 323 30 209 3.38e-13 69.7
MS.gene66338.t1 AT2G47490 29.814 161 95 6 66 220 159 307 8.01e-11 62.4
MS.gene66338.t1 AT5G64970 27.891 294 168 6 25 296 133 404 8.50e-21 92.4
MS.gene66338.t1 AT5G66380 26.603 312 189 10 26 315 8 301 2.23e-18 84.3
MS.gene66338.t1 AT1G74240 27.114 343 216 10 1 315 1 337 5.00e-18 84.3
MS.gene66338.t1 AT2G30160 25.083 303 199 6 23 315 38 322 1.02e-17 82.8
MS.gene66338.t1 AT5G46800 25.926 297 205 7 28 314 8 299 4.03e-17 80.9
MS.gene66338.t1 AT5G46800 25.926 297 205 7 28 314 8 299 4.03e-17 80.9
MS.gene66338.t1 AT5G46800 25.926 297 205 7 28 314 8 299 4.03e-17 80.9
MS.gene66338.t1 AT1G07030 23.934 305 197 6 23 315 36 317 3.84e-16 78.2
MS.gene66338.t1 AT2G33820 23.920 301 200 10 23 308 14 300 7.98e-16 77.0
MS.gene66338.t1 AT1G34065 29.146 199 123 4 26 224 155 335 7.62e-15 74.7
MS.gene66338.t1 AT1G34065 22.148 298 182 9 25 314 73 328 2.72e-11 64.3
MS.gene66338.t1 AT1G34065 29.146 199 123 4 26 224 162 342 8.16e-15 74.7
MS.gene66338.t1 AT1G34065 22.148 298 182 9 25 314 80 335 3.35e-11 63.9
MS.gene66338.t1 AT2G39970 24.497 298 197 8 40 310 21 317 1.46e-14 73.9
MS.gene66338.t1 AT1G79900 31.016 187 103 5 30 211 112 277 7.38e-14 71.2
MS.gene66338.t1 AT2G33820 23.301 309 200 11 23 308 14 308 2.71e-13 70.1
MS.gene66338.t1 AT4G27940 22.957 257 183 5 69 314 155 407 1.24e-12 68.6
MS.gene66338.t1 AT4G39460 21.975 314 199 7 7 314 38 311 1.49e-12 67.8
MS.gene66338.t1 AT4G39460 28.571 203 124 6 18 219 131 313 2.51e-12 67.0
MS.gene66338.t1 AT4G39460 21.975 314 199 7 7 314 38 311 1.49e-12 67.8
MS.gene66338.t1 AT4G39460 28.571 203 124 6 18 219 131 313 2.51e-12 67.0
MS.gene66338.t1 AT4G39460 21.975 314 199 7 7 314 38 311 1.49e-12 67.8
MS.gene66338.t1 AT4G39460 28.571 203 124 6 18 219 131 313 2.51e-12 67.0
MS.gene66338.t1 AT5G42130 24.752 303 191 9 36 323 127 407 1.87e-12 68.2
MS.gene66338.t1 AT5G42130 23.364 214 150 4 14 223 196 399 2.68e-11 64.3
MS.gene66338.t1 AT5G26200 24.138 319 217 7 9 311 25 334 2.95e-12 67.0
MS.gene66338.t1 AT1G74240 24.490 294 199 5 40 315 1 289 4.77e-12 66.2
MS.gene66338.t1 AT2G46320 26.772 254 165 9 69 311 109 352 8.22e-12 65.9
MS.gene66338.t1 AT5G58970 21.543 311 204 8 23 315 12 300 1.86e-11 64.3
MS.gene66338.t1 AT5G58970 25.000 204 130 4 17 215 111 296 6.02e-11 62.8
MS.gene66338.t1 AT2G46320 26.848 257 167 9 66 311 7 253 3.06e-11 63.2
MS.gene66338.t1 AT2G46320 26.848 257 167 9 66 311 7 253 3.06e-11 63.2
MS.gene66338.t1 AT2G46320 26.848 257 167 9 66 311 7 253 3.06e-11 63.2
MS.gene66338.t1 AT3G54110 27.895 190 118 7 137 317 29 208 6.00e-11 62.8

Find 94 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATCCGTGGCCGGTTTAT+TGG 0.168335 6.4:+63175201 MS.gene66338:CDS
CCCCAAAAGGCCTTCGGTTT+TGG 0.201446 6.4:-63174438 None:intergenic
CGCCAAAACCGAAGGCCTTT+TGG 0.218872 6.4:+63174436 MS.gene66338:CDS
CCAACCAAGTTGAATGTTTC+CGG 0.221472 6.4:+63174865 MS.gene66338:CDS
ACGTCTCAAAATTCTCTTTC+AGG 0.226178 6.4:+63172968 MS.gene66338:CDS
CTTCTTGCTGGTGGTCTTGC+TGG 0.240016 6.4:+63172912 MS.gene66338:CDS
TCGGACTAAGTTAGCTTATC+AGG 0.253353 6.4:+63174729 MS.gene66338:CDS
TGCAAGCCTTTCCTAATGTT+TGG 0.291574 6.4:+63174626 MS.gene66338:CDS
CTTATCCACTTGACTTAATT+CGG 0.304027 6.4:+63174710 MS.gene66338:CDS
ACCACCAGCAAGAAGTTCTT+TGG 0.324787 6.4:-63172904 None:intergenic
GCCACAGCTCCGACACTCTT+TGG 0.326066 6.4:+63175079 MS.gene66338:intron
GAGAGGGATGTGATCGAGTA+TGG 0.340180 6.4:-63172877 None:intergenic
TAGTCCACTTCATCTCTTGA+TGG 0.341198 6.4:-63175617 None:intergenic
GGATCCGTGGCCGGTTTATT+GGG 0.353057 6.4:+63175202 MS.gene66338:CDS
TTTGCCAAAGAACTTCTTGC+TGG 0.360123 6.4:+63172900 MS.gene66338:CDS
CGCTACGAAACTCAGTTCTT+CGG 0.375913 6.4:-63174385 None:intergenic
TGGCATTAGAGGTCTCTATA+GGG 0.376779 6.4:+63174954 MS.gene66338:CDS
TGATACTCAGCCCTGAAAAC+AGG 0.379917 6.4:-63175435 None:intergenic
GTGGCATTAGAGGTCTCTAT+AGG 0.387290 6.4:+63174953 MS.gene66338:CDS
CATATCCTCTTGAAGTTGTC+AGG 0.390609 6.4:+63175236 MS.gene66338:CDS
TATAAAGAGGGTGGCATTAG+AGG 0.393834 6.4:+63174943 MS.gene66338:CDS
GTAGGACCTTTCCAAACATT+AGG 0.400870 6.4:-63174637 None:intergenic
GAAGTTATTTGCAGGGGAAA+TGG 0.401049 6.4:+63174529 MS.gene66338:intron
TTTCGTAGCGCAGGACTGTC+TGG 0.406606 6.4:+63174398 MS.gene66338:CDS
CAGAGAGTGACCCTGCCATT+AGG 0.418512 6.4:-63174666 None:intergenic
AGGTCCTACCCTTGACCTAA+TGG 0.419290 6.4:+63174651 MS.gene66338:CDS
CAATCTTCGGTATTCCTCAT+AGG 0.426870 6.4:-63174599 None:intergenic
CAACCAAGTTGAATGTTTCC+GGG 0.429777 6.4:+63174866 MS.gene66338:CDS
CTAATGGCAGGGTCACTCTC+TGG 0.433291 6.4:+63174667 MS.gene66338:CDS
GTTGTTCCATCTGCGGCCAT+TGG 0.439759 6.4:+63175554 MS.gene66338:CDS
TCTCATAGTCCCTTTCAATT+CGG 0.446254 6.4:-63175374 None:intergenic
TTTGAAATTCTACTTCTATG+AGG 0.450212 6.4:+63175120 MS.gene66338:CDS
TAGAATTTCAAACCCGCGTA+TGG 0.453141 6.4:-63175109 None:intergenic
GCTACGAAACTCAGTTCTTC+GGG 0.457628 6.4:-63174384 None:intergenic
GCTGAGTATCAATTACATAA+AGG 0.458191 6.4:+63175447 MS.gene66338:CDS
CCGGAAACATTCAACTTGGT+TGG 0.459591 6.4:-63174865 None:intergenic
TCTTGTAATCCTCAGGTACA+TGG 0.471485 6.4:-63175153 None:intergenic
GCCAAAGAACTTCTTGCTGG+TGG 0.476313 6.4:+63172903 MS.gene66338:CDS
CATCCCGGAAACATTCAACT+TGG 0.482957 6.4:-63174869 None:intergenic
ATAAAATGAAGGCCTGCATA+AGG 0.494711 6.4:-63174574 None:intergenic
GCAAGGATTATTCCTTATGC+AGG 0.495081 6.4:+63174562 MS.gene66338:CDS
TGATTGCTCAAAAGCAAGGC+TGG 0.499574 6.4:+63175407 MS.gene66338:CDS
GTAGAACCCCAAAAGGCCTT+CGG 0.502499 6.4:-63174444 None:intergenic
TTGTTCATTATTTATCATCC+CGG 0.504892 6.4:-63174884 None:intergenic
CTTGCTGGTGGTCTTGCTGG+TGG 0.505519 6.4:+63172915 MS.gene66338:CDS
GCCTTTCCTAATGTTTGGAA+AGG 0.519689 6.4:+63174631 MS.gene66338:CDS
CTGAAGAAGCCGAATTGAAA+GGG 0.520218 6.4:+63175365 MS.gene66338:CDS
CAGGACTGTCTGGATCAGTT+AGG 0.522724 6.4:+63174408 MS.gene66338:CDS
AGGCTTGCATGATCAATCTT+CGG 0.527031 6.4:-63174612 None:intergenic
CCTACCCTTGACCTAATGGC+AGG 0.530791 6.4:+63174655 MS.gene66338:CDS
AGGGTCACTCTCTGGAGGGA+CGG 0.530967 6.4:+63174675 MS.gene66338:CDS
AGTACCATCAAGAGATGAAG+TGG 0.531658 6.4:+63175613 MS.gene66338:CDS
GAATGTCTGACCCAATAAAC+CGG 0.532252 6.4:-63175212 None:intergenic
GTGAAGCCAATGGCCGCAGA+TGG 0.532601 6.4:-63175560 None:intergenic
CTCACATGTGGATCCGTGGC+CGG 0.536964 6.4:+63175193 MS.gene66338:CDS
ACCTTTCCAAACATTAGGAA+AGG 0.538316 6.4:-63174632 None:intergenic
AGAAGTTCTTTGGCAAAGAG+AGG 0.540450 6.4:-63172894 None:intergenic
AGAGGTCTCTATAGGGGAGT+AGG 0.544211 6.4:+63174961 MS.gene66338:CDS
TCTGAAGAAGCCGAATTGAA+AGG 0.545458 6.4:+63175364 MS.gene66338:CDS
CGGCTTCTTCAGATGCCGCA+AGG 0.546244 6.4:-63175354 None:intergenic
AGAACTGAGTTTCGTAGCGC+AGG 0.553711 6.4:+63174389 MS.gene66338:CDS
CCTCATAGGACATAAAATGA+AGG 0.555164 6.4:-63174585 None:intergenic
TTAGTCCGAATTAAGTCAAG+TGG 0.558808 6.4:-63174715 None:intergenic
AGAATTTCAAACCCGCGTAT+GGG 0.562448 6.4:-63175108 None:intergenic
AGTTGTCAGGAGGCAAATGC+AGG 0.565986 6.4:+63175249 MS.gene66338:CDS
TCTACCTGTAGAACCCCAAA+AGG 0.568287 6.4:-63174451 None:intergenic
AATTCAGGTTGTTCCATCTG+CGG 0.571625 6.4:+63175547 MS.gene66338:intron
CAGGGGAAATGGAGCAAGTG+TGG 0.576065 6.4:+63174540 MS.gene66338:intron
GAAAGGGACTATGAGATCCA+TGG 0.576797 6.4:+63175381 MS.gene66338:CDS
TGCCTCCTGACAACTTCAAG+AGG 0.577185 6.4:-63175241 None:intergenic
GTGACCCTGCCATTAGGTCA+AGG 0.580517 6.4:-63174660 None:intergenic
ATAATGAACAAGTTTATCGA+GGG 0.581693 6.4:+63174896 MS.gene66338:CDS
CTTTGGAATATTCCCATACG+CGG 0.582745 6.4:+63175096 MS.gene66338:CDS
GAAGTTCTTTGGCAAAGAGA+GGG 0.587372 6.4:-63172893 None:intergenic
GGCATTAGAGGTCTCTATAG+GGG 0.605826 6.4:+63174955 MS.gene66338:CDS
AATAATGAACAAGTTTATCG+AGG 0.610108 6.4:+63174895 MS.gene66338:CDS
GATACTCAGCCCTGAAAACA+GGG 0.610200 6.4:-63175434 None:intergenic
ATGGCAGGGTCACTCTCTGG+AGG 0.610692 6.4:+63174670 MS.gene66338:CDS
TGACCCTGCCATTAGGTCAA+GGG 0.610872 6.4:-63174659 None:intergenic
GAAACTCACATGTGGATCCG+TGG 0.613310 6.4:+63175189 MS.gene66338:CDS
GGGAATATTCCAAAGAGTGT+CGG 0.616862 6.4:-63175088 None:intergenic
AGGAGAATCGCCAAAACCGA+AGG 0.618278 6.4:+63174428 MS.gene66338:CDS
ATCATAAACTGTGAAGCCAA+TGG 0.621650 6.4:-63175570 None:intergenic
CTGACCCAATAAACCGGCCA+CGG 0.624628 6.4:-63175206 None:intergenic
GAAATGGAGCAAGTGTGGCA+AGG 0.627350 6.4:+63174545 MS.gene66338:CDS
CTACCCTTGACCTAATGGCA+GGG 0.629539 6.4:+63174656 MS.gene66338:CDS
TGGCAGGGTCACTCTCTGGA+GGG 0.638491 6.4:+63174671 MS.gene66338:CDS
TTTGGAATATTCCCATACGC+GGG 0.647148 6.4:+63175097 MS.gene66338:CDS
TGACTAGGTTCAAAACCTTG+CGG 0.662992 6.4:+63175339 MS.gene66338:intron
AATGAAGCGCCATGTACCTG+AGG 0.663160 6.4:+63175144 MS.gene66338:CDS
CCTGCCATTAGGTCAAGGGT+AGG 0.672225 6.4:-63174655 None:intergenic
ATCCTCTTGAAGTTGTCAGG+AGG 0.680873 6.4:+63175239 MS.gene66338:CDS
ATCGTGGCGAAACTCACATG+TGG 0.699503 6.4:+63175181 MS.gene66338:CDS
GGATTACAAGAAAAGCATCG+TGG 0.780954 6.4:+63175165 MS.gene66338:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTTGAGAAAATGAAAT+TGG + chr6.4:63173996-63174015 MS.gene66338:intron 15.0%
!!! TCATTTTCTCAAATTATTAT+CGG - chr6.4:63173993-63174012 None:intergenic 15.0%
!! AATAATTAGATATGTTGTGT+TGG + chr6.4:63173495-63173514 MS.gene66338:intron 20.0%
!! AGTTGAGAATGATAATTATA+AGG - chr6.4:63174168-63174187 None:intergenic 20.0%
!! CTTTCAAAAACTTATAAAGA+GGG + chr6.4:63174931-63174950 MS.gene66338:CDS 20.0%
!! GTTGAGAATGATAATTATAA+GGG - chr6.4:63174167-63174186 None:intergenic 20.0%
!! TAACAAAAGAAAAAACAAGA+AGG - chr6.4:63173119-63173138 None:intergenic 20.0%
!! TCTTTCAAAAACTTATAAAG+AGG + chr6.4:63174930-63174949 MS.gene66338:CDS 20.0%
!!! TCATTTTCTCATATCATTAT+CGG - chr6.4:63173057-63173076 None:intergenic 20.0%
!!! TTTTTTTTTTGTTGCAATTC+AGG + chr6.4:63175532-63175551 MS.gene66338:intron 20.0%
! AAATGCTTAGTTATAGAGAA+CGG - chr6.4:63175514-63175533 None:intergenic 25.0%
! AATAATGAACAAGTTTATCG+AGG + chr6.4:63174895-63174914 MS.gene66338:CDS 25.0%
! AGTATCAATTACATAAAGGT+GGG + chr6.4:63175451-63175470 MS.gene66338:intron 25.0%
! ATAATGAACAAGTTTATCGA+GGG + chr6.4:63174896-63174915 MS.gene66338:CDS 25.0%
! GATTGCAATTAATCAATCTA+TGG - chr6.4:63174352-63174371 None:intergenic 25.0%
! TAGAACTCTTATGTTAATCA+TGG + chr6.4:63175057-63175076 MS.gene66338:intron 25.0%
! TAGTTATAGTTACAGACATA+AGG + chr6.4:63173762-63173781 MS.gene66338:intron 25.0%
! TATGAGTTAGTTAGTTAGTT+AGG + chr6.4:63173352-63173371 MS.gene66338:intron 25.0%
! TTAATCAATCTATGGTCAAT+TGG - chr6.4:63174344-63174363 None:intergenic 25.0%
! TTGAATCATTGAAAGTCAAT+GGG + chr6.4:63173588-63173607 MS.gene66338:intron 25.0%
! TTGTTCATTATTTATCATCC+CGG - chr6.4:63174887-63174906 None:intergenic 25.0%
! TTTGAATTTCTTACGAAAGA+GGG + chr6.4:63173260-63173279 MS.gene66338:intron 25.0%
!! TTTGAAATTCTACTTCTATG+AGG + chr6.4:63175120-63175139 MS.gene66338:CDS 25.0%
!! TTTTGAATTTCTTACGAAAG+AGG + chr6.4:63173259-63173278 MS.gene66338:intron 25.0%
!!! ATTATTATTGATGTCGACTT+CGG - chr6.4:63173873-63173892 None:intergenic 25.0%
ACAAAATACAACCAAAGTCA+TGG - chr6.4:63174794-63174813 None:intergenic 30.0%
ACTAGCATAAAACCTACAAT+AGG - chr6.4:63173791-63173810 None:intergenic 30.0%
CTAGCATAAAACCTACAATA+GGG - chr6.4:63173790-63173809 None:intergenic 30.0%
CTTATCCACTTGACTTAATT+CGG + chr6.4:63174710-63174729 MS.gene66338:CDS 30.0%
CTTATGTAACAGTAATGGTT+AGG - chr6.4:63173657-63173676 None:intergenic 30.0%
CTTGAATCATTGAAAGTCAA+TGG + chr6.4:63173587-63173606 MS.gene66338:intron 30.0%
GAGTATCAATTACATAAAGG+TGG + chr6.4:63175450-63175469 MS.gene66338:intron 30.0%
GCTAGTAAAAAATGAGATCT+TGG + chr6.4:63173815-63173834 MS.gene66338:intron 30.0%
GCTGAGTATCAATTACATAA+AGG + chr6.4:63175447-63175466 MS.gene66338:CDS 30.0%
TACAGGTAGATAGAAATCAT+AGG + chr6.4:63174464-63174483 MS.gene66338:intron 30.0%
TCAAAAACTTATAAAGAGGG+TGG + chr6.4:63174934-63174953 MS.gene66338:CDS 30.0%
TCACTCTTATGTAACAGTAA+TGG - chr6.4:63173662-63173681 None:intergenic 30.0%
TGAATCATTGAAAGTCAATG+GGG + chr6.4:63173589-63173608 MS.gene66338:intron 30.0%
! AAGAGGGTAACTTTTTATGA+AGG + chr6.4:63173276-63173295 MS.gene66338:intron 30.0%
! ACTAACTTACTAACTGATTC+AGG - chr6.4:63173746-63173765 None:intergenic 30.0%
! GATACAAAGTTAACTTGACT+AGG + chr6.4:63175324-63175343 MS.gene66338:intron 30.0%
!! AAGTTTTTGAAAGACAGTCA+CGG - chr6.4:63174924-63174943 None:intergenic 30.0%
!!! GTACAGTTACAGACATTTTT+TGG + chr6.4:63173374-63173393 MS.gene66338:intron 30.0%
ACCAATGTGAAGTTATTTGC+AGG + chr6.4:63174521-63174540 MS.gene66338:intron 35.0%
ACCTTTCCAAACATTAGGAA+AGG - chr6.4:63174635-63174654 None:intergenic 35.0%
ACGTCTCAAAATTCTCTTTC+AGG + chr6.4:63172968-63172987 MS.gene66338:CDS 35.0%
ATAAAATGAAGGCCTGCATA+AGG - chr6.4:63174577-63174596 None:intergenic 35.0%
ATCATAAACTGTGAAGCCAA+TGG - chr6.4:63175573-63175592 None:intergenic 35.0%
CAATGTGAAGTTATTTGCAG+GGG + chr6.4:63174523-63174542 MS.gene66338:intron 35.0%
CAGATAAATAGAGATGTGTC+TGG - chr6.4:63173543-63173562 None:intergenic 35.0%
CATTGCATGCAATTAGTGAA+TGG + chr6.4:63174299-63174318 MS.gene66338:intron 35.0%
CATTTAAATCTGCTTGTGTC+TGG + chr6.4:63174988-63175007 MS.gene66338:intron 35.0%
CCAATGTGAAGTTATTTGCA+GGG + chr6.4:63174522-63174541 MS.gene66338:intron 35.0%
CCTCATAGGACATAAAATGA+AGG - chr6.4:63174588-63174607 None:intergenic 35.0%
CTAACTAACTCATACAAGTG+AGG - chr6.4:63173346-63173365 None:intergenic 35.0%
GGTAGATAGAAATCATAGGT+AGG + chr6.4:63174468-63174487 MS.gene66338:intron 35.0%
TCTCATAGTCCCTTTCAATT+CGG - chr6.4:63175377-63175396 None:intergenic 35.0%
TGAATGACACTGAAACTCAA+TGG + chr6.4:63173202-63173221 MS.gene66338:intron 35.0%
TTAGTCCGAATTAAGTCAAG+TGG - chr6.4:63174718-63174737 None:intergenic 35.0%
! ATGCTTTTCTTGTAATCCTC+AGG - chr6.4:63175163-63175182 None:intergenic 35.0%
! CCTTCATTTTATGTCCTATG+AGG + chr6.4:63174585-63174604 MS.gene66338:CDS 35.0%
! CTCAAAATTCTCTTTCAGGT+AGG + chr6.4:63172972-63172991 MS.gene66338:intron 35.0%
! CTTTTGAGCAATCAAAACCA+TGG - chr6.4:63175401-63175420 None:intergenic 35.0%
!! TGTCAATTTGTGTTTGGTGT+TGG - chr6.4:63173962-63173981 None:intergenic 35.0%
!!! AGAATTTTGAGACGTTCAAG+AGG - chr6.4:63172963-63172982 None:intergenic 35.0%
!!! GTTTTGATTGCTCAAAAGCA+AGG + chr6.4:63175403-63175422 MS.gene66338:CDS 35.0%
AAGAAGGAAGCTATGTAGCA+CGG - chr6.4:63173103-63173122 None:intergenic 40.0%
AGAAGTTCTTTGGCAAAGAG+AGG - chr6.4:63172897-63172916 None:intergenic 40.0%
AGAATTTCAAACCCGCGTAT+GGG - chr6.4:63175111-63175130 None:intergenic 40.0%
AGGCTTGCATGATCAATCTT+CGG - chr6.4:63174615-63174634 None:intergenic 40.0%
AGTACCATCAAGAGATGAAG+TGG + chr6.4:63175613-63175632 MS.gene66338:CDS 40.0%
CAATCTTCGGTATTCCTCAT+AGG - chr6.4:63174602-63174621 None:intergenic 40.0%
CATATCCTCTTGAAGTTGTC+AGG + chr6.4:63175236-63175255 MS.gene66338:CDS 40.0%
CCCTGCAAATAACTTCACAT+TGG - chr6.4:63174525-63174544 None:intergenic 40.0%
CTGAAGAAGCCGAATTGAAA+GGG + chr6.4:63175365-63175384 MS.gene66338:CDS 40.0%
GAAGTTATTTGCAGGGGAAA+TGG + chr6.4:63174529-63174548 MS.gene66338:intron 40.0%
GAAGTTCTTTGGCAAAGAGA+GGG - chr6.4:63172896-63172915 None:intergenic 40.0%
GAATGTCTGACCCAATAAAC+CGG - chr6.4:63175215-63175234 None:intergenic 40.0%
GCAAGGATTATTCCTTATGC+AGG + chr6.4:63174562-63174581 MS.gene66338:CDS 40.0%
GCCTTTCCTAATGTTTGGAA+AGG + chr6.4:63174631-63174650 MS.gene66338:CDS 40.0%
GTAGGACCTTTCCAAACATT+AGG - chr6.4:63174640-63174659 None:intergenic 40.0%
TAGAATTTCAAACCCGCGTA+TGG - chr6.4:63175112-63175131 None:intergenic 40.0%
TAGTCCACTTCATCTCTTGA+TGG - chr6.4:63175620-63175639 None:intergenic 40.0%
TATAAAGAGGGTGGCATTAG+AGG + chr6.4:63174943-63174962 MS.gene66338:CDS 40.0%
TCGTCGTGTCAATTTGTGTT+TGG - chr6.4:63173968-63173987 None:intergenic 40.0%
TCTGAAGAAGCCGAATTGAA+AGG + chr6.4:63175364-63175383 MS.gene66338:CDS 40.0%
TCTTGTAATCCTCAGGTACA+TGG - chr6.4:63175156-63175175 None:intergenic 40.0%
TGACTAGGTTCAAAACCTTG+CGG + chr6.4:63175339-63175358 MS.gene66338:intron 40.0%
TGCAAGCCTTTCCTAATGTT+TGG + chr6.4:63174626-63174645 MS.gene66338:CDS 40.0%
TGGCATTAGAGGTCTCTATA+GGG + chr6.4:63174954-63174973 MS.gene66338:CDS 40.0%
TTTGCCAAAGAACTTCTTGC+TGG + chr6.4:63172900-63172919 MS.gene66338:CDS 40.0%
! AATCTGCTTGTGTCTGGAAT+TGG + chr6.4:63174994-63175013 MS.gene66338:intron 40.0%
! CAACCAAGTTGAATGTTTCC+GGG + chr6.4:63174866-63174885 MS.gene66338:CDS 40.0%
! CCAACCAAGTTGAATGTTTC+CGG + chr6.4:63174865-63174884 MS.gene66338:CDS 40.0%
! CTCATATCATTATCGGTGTC+CGG - chr6.4:63173050-63173069 None:intergenic 40.0%
! GACATAAGGTACCCTATTGT+AGG + chr6.4:63173776-63173795 MS.gene66338:intron 40.0%
! GGGAATATTCCAAAGAGTGT+CGG - chr6.4:63175091-63175110 None:intergenic 40.0%
! GTCGGATATCTGAGAAGTAT+CGG + chr6.4:63174066-63174085 MS.gene66338:intron 40.0%
! TCAGTTTTCGTCCATGACTT+TGG + chr6.4:63174780-63174799 MS.gene66338:intron 40.0%
! TCGGACTAAGTTAGCTTATC+AGG + chr6.4:63174729-63174748 MS.gene66338:CDS 40.0%
! TGCAGGTAACGTGCTTTATT+TGG + chr6.4:63175266-63175285 MS.gene66338:intron 40.0%
! TTTGGAATATTCCCATACGC+GGG + chr6.4:63175097-63175116 MS.gene66338:CDS 40.0%
!! AATTCAGGTTGTTCCATCTG+CGG + chr6.4:63175547-63175566 MS.gene66338:intron 40.0%
!! CTTTGGAATATTCCCATACG+CGG + chr6.4:63175096-63175115 MS.gene66338:CDS 40.0%
!! GGATTACAAGAAAAGCATCG+TGG + chr6.4:63175165-63175184 MS.gene66338:CDS 40.0%
!! GGTAACGTGCTTTATTTGGT+CGG + chr6.4:63175270-63175289 MS.gene66338:intron 40.0%
!! TGAAGCATTGACACTAGACA+CGG + chr6.4:63173844-63173863 MS.gene66338:intron 40.0%
!!! CAGACATTTTTTGGTTCTCG+AGG + chr6.4:63173383-63173402 MS.gene66338:intron 40.0%
AATGGTCGGAAACAGAGACA+TGG + chr6.4:63174271-63174290 MS.gene66338:intron 45.0%
ACCACCAGCAAGAAGTTCTT+TGG - chr6.4:63172907-63172926 None:intergenic 45.0%
ATCCTCTTGAAGTTGTCAGG+AGG + chr6.4:63175239-63175258 MS.gene66338:CDS 45.0%
CATCCCGGAAACATTCAACT+TGG - chr6.4:63174872-63174891 None:intergenic 45.0%
CCGGAAACATTCAACTTGGT+TGG - chr6.4:63174868-63174887 None:intergenic 45.0%
CGCTACGAAACTCAGTTCTT+CGG - chr6.4:63174388-63174407 None:intergenic 45.0%
GAAAGGGACTATGAGATCCA+TGG + chr6.4:63175381-63175400 MS.gene66338:CDS 45.0%
GATACTCAGCCCTGAAAACA+GGG - chr6.4:63175437-63175456 None:intergenic 45.0%
GCTACGAAACTCAGTTCTTC+GGG - chr6.4:63174387-63174406 None:intergenic 45.0%
GGCATTAGAGGTCTCTATAG+GGG + chr6.4:63174955-63174974 MS.gene66338:CDS 45.0%
GGTCGGCTGGAAATTATAGA+TGG + chr6.4:63175287-63175306 MS.gene66338:intron 45.0%
GTGGCATTAGAGGTCTCTAT+AGG + chr6.4:63174953-63174972 MS.gene66338:CDS 45.0%
GTGTAAGATTGCGCAAGTGT+GGG + chr6.4:63174243-63174262 MS.gene66338:intron 45.0%
TCAATATCAACGTGTCGTGC+TGG - chr6.4:63173446-63173465 None:intergenic 45.0%
TCTACCTGTAGAACCCCAAA+AGG - chr6.4:63174454-63174473 None:intergenic 45.0%
TGATACTCAGCCCTGAAAAC+AGG - chr6.4:63175438-63175457 None:intergenic 45.0%
TGATTGCTCAAAAGCAAGGC+TGG + chr6.4:63175407-63175426 MS.gene66338:CDS 45.0%
TGTAAGATTGCGCAAGTGTG+GGG + chr6.4:63174244-63174263 MS.gene66338:intron 45.0%
TGTGTAAGATTGCGCAAGTG+TGG + chr6.4:63174242-63174261 MS.gene66338:intron 45.0%
! AAGTGTGGGGAGATAATGGT+CGG + chr6.4:63174257-63174276 MS.gene66338:intron 45.0%
! ACGTCTTCCACAAGAAGTGT+CGG + chr6.4:63174091-63174110 MS.gene66338:intron 45.0%
! ATATCATTATCGGTGTCCGG+TGG - chr6.4:63173047-63173066 None:intergenic 45.0%
! CTCGAGGAAAGTACAGTTAC+AGG + chr6.4:63173399-63173418 MS.gene66338:intron 45.0%
!! GAAGCATTGACACTAGACAC+GGG + chr6.4:63173845-63173864 MS.gene66338:intron 45.0%
!! TTGTGTTGGTGTCATCGAGA+CGG + chr6.4:63173509-63173528 MS.gene66338:intron 45.0%
AGAACTGAGTTTCGTAGCGC+AGG + chr6.4:63174389-63174408 MS.gene66338:CDS 50.0%
AGAGGTCTCTATAGGGGAGT+AGG + chr6.4:63174961-63174980 MS.gene66338:CDS 50.0%
AGGAGAATCGCCAAAACCGA+AGG + chr6.4:63174428-63174447 MS.gene66338:CDS 50.0%
AGGTCCTACCCTTGACCTAA+TGG + chr6.4:63174651-63174670 MS.gene66338:CDS 50.0%
AGTTGTCAGGAGGCAAATGC+AGG + chr6.4:63175249-63175268 MS.gene66338:CDS 50.0%
ATCGTGGCGAAACTCACATG+TGG + chr6.4:63175181-63175200 MS.gene66338:CDS 50.0%
CAGGACTGTCTGGATCAGTT+AGG + chr6.4:63174408-63174427 MS.gene66338:CDS 50.0%
CTACCCTTGACCTAATGGCA+GGG + chr6.4:63174656-63174675 MS.gene66338:CDS 50.0%
GAAACTCACATGTGGATCCG+TGG + chr6.4:63175189-63175208 MS.gene66338:CDS 50.0%
GAAATGGAGCAAGTGTGGCA+AGG + chr6.4:63174545-63174564 MS.gene66338:CDS 50.0%
GAGAGGGATGTGATCGAGTA+TGG - chr6.4:63172880-63172899 None:intergenic 50.0%
GCCAAAGAACTTCTTGCTGG+TGG + chr6.4:63172903-63172922 MS.gene66338:CDS 50.0%
GTAGAACCCCAAAAGGCCTT+CGG - chr6.4:63174447-63174466 None:intergenic 50.0%
GTCAATGCTGGACAACGACA+CGG - chr6.4:63174042-63174061 None:intergenic 50.0%
TATCCGACACGTGTCAATGC+TGG - chr6.4:63174054-63174073 None:intergenic 50.0%
TGACCCTGCCATTAGGTCAA+GGG - chr6.4:63174662-63174681 None:intergenic 50.0%
TGCCTCCTGACAACTTCAAG+AGG - chr6.4:63175244-63175263 None:intergenic 50.0%
! AATGAAGCGCCATGTACCTG+AGG + chr6.4:63175144-63175163 MS.gene66338:CDS 50.0%
! CCAAAACCGAAGGCCTTTTG+GGG + chr6.4:63174438-63174457 MS.gene66338:CDS 50.0%
! GCCAAAACCGAAGGCCTTTT+GGG + chr6.4:63174437-63174456 MS.gene66338:CDS 50.0%
! GCTGGAAGACCCTGTTTTCA+GGG + chr6.4:63175425-63175444 MS.gene66338:CDS 50.0%
! TGTCGGTGCTTCGCGTAAAA+AGG - chr6.4:63173018-63173037 None:intergenic 50.0%
!! ACGTGCTTTATTTGGTCGGC+TGG + chr6.4:63175274-63175293 MS.gene66338:intron 50.0%
!! TGTCCAGCATTGACACGTGT+CGG + chr6.4:63174048-63174067 MS.gene66338:intron 50.0%
!!! AAGGCCTTTTGGGGTTCTAC+AGG + chr6.4:63174447-63174466 MS.gene66338:CDS 50.0%
CAGAGAGTGACCCTGCCATT+AGG - chr6.4:63174669-63174688 None:intergenic 55.0%
CAGGGGAAATGGAGCAAGTG+TGG + chr6.4:63174540-63174559 MS.gene66338:intron 55.0%
CCTACCCTTGACCTAATGGC+AGG + chr6.4:63174655-63174674 MS.gene66338:CDS 55.0%
CCTGCCATTAGGTCAAGGGT+AGG - chr6.4:63174658-63174677 None:intergenic 55.0%
CTAATGGCAGGGTCACTCTC+TGG + chr6.4:63174667-63174686 MS.gene66338:CDS 55.0%
CTGACCCAATAAACCGGCCA+CGG - chr6.4:63175209-63175228 None:intergenic 55.0%
GCGCAAGTGTGGGGAGATAA+TGG + chr6.4:63174253-63174272 MS.gene66338:intron 55.0%
GTGACCCTGCCATTAGGTCA+AGG - chr6.4:63174663-63174682 None:intergenic 55.0%
GTTGTTCCATCTGCGGCCAT+TGG + chr6.4:63175554-63175573 MS.gene66338:CDS 55.0%
TTTCGTAGCGCAGGACTGTC+TGG + chr6.4:63174398-63174417 MS.gene66338:CDS 55.0%
! CCCCAAAAGGCCTTCGGTTT+TGG - chr6.4:63174441-63174460 None:intergenic 55.0%
! CGCCAAAACCGAAGGCCTTT+TGG + chr6.4:63174436-63174455 MS.gene66338:CDS 55.0%
! CTTCTTGCTGGTGGTCTTGC+TGG + chr6.4:63172912-63172931 MS.gene66338:CDS 55.0%
! GGATCCGTGGCCGGTTTATT+GGG + chr6.4:63175202-63175221 MS.gene66338:CDS 55.0%
! GGCTGGAAGACCCTGTTTTC+AGG + chr6.4:63175424-63175443 MS.gene66338:CDS 55.0%
! TGGATCCGTGGCCGGTTTAT+TGG + chr6.4:63175201-63175220 MS.gene66338:CDS 55.0%
!! CGTAGCACCGACACTTCTTG+TGG - chr6.4:63174101-63174120 None:intergenic 55.0%
!! TCCAAAGAGTGTCGGAGCTG+TGG - chr6.4:63175083-63175102 None:intergenic 55.0%
ATGGCAGGGTCACTCTCTGG+AGG + chr6.4:63174670-63174689 MS.gene66338:CDS 60.0%
CGGCTTCTTCAGATGCCGCA+AGG - chr6.4:63175357-63175376 None:intergenic 60.0%
CTCACATGTGGATCCGTGGC+CGG + chr6.4:63175193-63175212 MS.gene66338:CDS 60.0%
GCCACAGCTCCGACACTCTT+TGG + chr6.4:63175079-63175098 MS.gene66338:intron 60.0%
GTGAAGCCAATGGCCGCAGA+TGG - chr6.4:63175563-63175582 None:intergenic 60.0%
GTGTCCGGTGGTGTCTGTGT+CGG - chr6.4:63173035-63173054 None:intergenic 60.0%
TGGCAGGGTCACTCTCTGGA+GGG + chr6.4:63174671-63174690 MS.gene66338:CDS 60.0%
! AGGGTCACTCTCTGGAGGGA+CGG + chr6.4:63174675-63174694 MS.gene66338:CDS 60.0%
! CTTGCTGGTGGTCTTGCTGG+TGG + chr6.4:63172915-63172934 MS.gene66338:CDS 60.0%
!! AGCACCGACACAGACACCAC+CGG + chr6.4:63173028-63173047 MS.gene66338:intron 60.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 63172834 63175640 63172834 ID=MS.gene66338
chr6.4 mRNA 63172834 63175640 63172834 ID=MS.gene66338.t1;Parent=MS.gene66338
chr6.4 exon 63172834 63172989 63172834 ID=MS.gene66338.t1.exon1;Parent=MS.gene66338.t1
chr6.4 CDS 63172834 63172989 63172834 ID=cds.MS.gene66338.t1;Parent=MS.gene66338.t1
chr6.4 exon 63174383 63174468 63174383 ID=MS.gene66338.t1.exon2;Parent=MS.gene66338.t1
chr6.4 CDS 63174383 63174468 63174383 ID=cds.MS.gene66338.t1;Parent=MS.gene66338.t1
chr6.4 exon 63174543 63174750 63174543 ID=MS.gene66338.t1.exon3;Parent=MS.gene66338.t1
chr6.4 CDS 63174543 63174750 63174543 ID=cds.MS.gene66338.t1;Parent=MS.gene66338.t1
chr6.4 exon 63174856 63174982 63174856 ID=MS.gene66338.t1.exon4;Parent=MS.gene66338.t1
chr6.4 CDS 63174856 63174982 63174856 ID=cds.MS.gene66338.t1;Parent=MS.gene66338.t1
chr6.4 exon 63175086 63175270 63175086 ID=MS.gene66338.t1.exon5;Parent=MS.gene66338.t1
chr6.4 CDS 63175086 63175270 63175086 ID=cds.MS.gene66338.t1;Parent=MS.gene66338.t1
chr6.4 exon 63175346 63175468 63175346 ID=MS.gene66338.t1.exon6;Parent=MS.gene66338.t1
chr6.4 CDS 63175346 63175468 63175346 ID=cds.MS.gene66338.t1;Parent=MS.gene66338.t1
chr6.4 exon 63175554 63175640 63175554 ID=MS.gene66338.t1.exon7;Parent=MS.gene66338.t1
chr6.4 CDS 63175554 63175640 63175554 ID=cds.MS.gene66338.t1;Parent=MS.gene66338.t1
Gene Sequence

>MS.gene66338

ATGGCAGCATCATCATCATCATCATCATCAGATGAGAGAGAATCCATACTCGATCACATCCCTCTCTTTGCCAAAGAACTTCTTGCTGGTGGTCTTGCTGGTGGCTTTGCTAAAACTCTTGTTGCTCCTCTTGAACGTCTCAAAATTCTCTTTCAGGTAGGTACTTTAATAGTTTAATTCTCCTTTTTACGCGAAGCACCGACACAGACACCACCGGACACCGATAATGATATGAGAAAATGACACAATTCTTTGATCAGACGTGTCCGTGCTACATAGCTTCCTTCTTGTTTTTTCTTTTGTTATGTTTTGAAATGTGTTTGTATGCTTTGATTTCATGTGTGATCTGTTAATGTTTGATTAATCTTTGAATGACACTGAAACTCAATGGAAAATTTTAGAGAATGATGAAATCTTGCTTAGTCTTTTGAATTTCTTACGAAAGAGGGTAACTTTTTATGAAGGTAGTTGAAACTTGAAATGCAGTTTAACTGCTTAGAATAAAAATACCTCACTTGTATGAGTTAGTTAGTTAGTTAGGTACAGTTACAGACATTTTTTGGTTCTCGAGGAAAGTACAGTTACAGGCATTAAGTACAAATGCAGACACCAGCACGACACGTTGATATTGATAATGATTTGAGAAAATGAATTAATTGAAAATAATTAGATATGTTGTGTTGGTGTCATCGAGACGGATCAGACACCAGACACATCTCTATTTATCTGTAATCTATCTTATGTTTGATATATCTTGAATCATTGAAAGTCAATGGGGAATTTTAGAGAATAACAAAATCTGAGTTTTTGCATTCTAAGTCCTAACCATTACTGTTACATAAGAGTGAAATTTTTTGTACATGTAGCTGATGAACTTTAACTGCTTAAATTAACTCGAAAAGTAACTCACCTGAATCAGTTAGTAAGTTAGTTATAGTTACAGACATAAGGTACCCTATTGTAGGTTTTATGCTAGTACTAGCTAGTAAAAAATGAGATCTTGGATACTATGAAGCATTGACACTAGACACGGGCTCCGAAGTCGACATCAATAATAATTTGAAAAAATGAATGAATTGAATGTAATCACGTATGAAGCATTGTGATTTTGAAAAATATGAAGCACCAACACCAAACACAAATTGACACGACGACACCGATAATAATTTGAGAAAATGAAATTGGATGTAATCACATATGTGATACCGTGTCGTTGTCCAGCATTGACACGTGTCGGATATCTGAGAAGTATCGGATACGTCTTCCACAAGAAGTGTCGGTGCTACGTGTAATGTAATAATTTTGATTATACATCTCAATTTTATTCTTTCCCTTATAATTATCATTCTCAACTTTTCACTTCTAGCAGTATTTTTCTGTTATGCATACACTAGTAAAGTAAAGATATTGTGTAAGATTGCGCAAGTGTGGGGAGATAATGGTCGGAAACAGAGACATGGAATTTCATTGCATGCAATTAGTGAATGGCTTTTCTTAAACTAAGCGACCAATTGACCATAGATTGATTAATTGCAATCATATTTTGCAGACCCGAAGAACTGAGTTTCGTAGCGCAGGACTGTCTGGATCAGTTAGGAGAATCGCCAAAACCGAAGGCCTTTTGGGGTTCTACAGGTAGATAGAAATCATAGGTAGGTGATTTATCTTTAATCAATTAATCAGAATCACCAATGTGAAGTTATTTGCAGGGGAAATGGAGCAAGTGTGGCAAGGATTATTCCTTATGCAGGCCTTCATTTTATGTCCTATGAGGAATACCGAAGATTGATCATGCAAGCCTTTCCTAATGTTTGGAAAGGTCCTACCCTTGACCTAATGGCAGGGTCACTCTCTGGAGGGACGGCTGTGCTTTTTACTTATCCACTTGACTTAATTCGGACTAAGTTAGCTTATCAGGTAAATTATATTTCTGCTCAGAAATCAAATCAGTTTTCGTCCATGACTTTGGTTGTATTTTGTGATTACATGAAGTGTTAACTTCATGTGAAATTGAAACTGCAGATTGTTAGTCCAACCAAGTTGAATGTTTCCGGGATGATAAATAATGAACAAGTTTATCGAGGGATCCGTGACTGTCTTTCAAAAACTTATAAAGAGGGTGGCATTAGAGGTCTCTATAGGGGAGTAGGTATGCATTTAAATCTGCTTGTGTCTGGAATTGGTAATTCAGCAGTATTGTATTTCACTGCTGTGTTGTTATTGTAGAACTCTTATGTTAATCATGGCCACAGCTCCGACACTCTTTGGAATATTCCCATACGCGGGTTTGAAATTCTACTTCTATGAGGAAATGAAGCGCCATGTACCTGAGGATTACAAGAAAAGCATCGTGGCGAAACTCACATGTGGATCCGTGGCCGGTTTATTGGGTCAGACATTCACATATCCTCTTGAAGTTGTCAGGAGGCAAATGCAGGTAACGTGCTTTATTTGGTCGGCTGGAAATTATAGATGGATATTGAGTAACATGATACAAAGTTAACTTGACTAGGTTCAAAACCTTGCGGCATCTGAAGAAGCCGAATTGAAAGGGACTATGAGATCCATGGTTTTGATTGCTCAAAAGCAAGGCTGGAAGACCCTGTTTTCAGGGCTGAGTATCAATTACATAAAGGTGGGAAAGTCATAGAACATTAAAGTAATTCACACTCTAGAACCGTTCTCTATAACTAAGCATTTTTTTTTTTGTTGCAATTCAGGTTGTTCCATCTGCGGCCATTGGCTTCACAGTTTATGATACTATGAAATCATACTTGAGAGTACCATCAAGAGATGAAGTGGACTAA
Protein sequence

>MS.gene66338.t1

MAASSSSSSSDERESILDHIPLFAKELLAGGLAGGFAKTLVAPLERLKILFQTRRTEFRSAGLSGSVRRIAKTEGLLGFYRGNGASVARIIPYAGLHFMSYEEYRRLIMQAFPNVWKGPTLDLMAGSLSGGTAVLFTYPLDLIRTKLAYQIVSPTKLNVSGMINNEQVYRGIRDCLSKTYKEGGIRGLYRGVAPTLFGIFPYAGLKFYFYEEMKRHVPEDYKKSIVAKLTCGSVAGLLGQTFTYPLEVVRRQMQVQNLAASEEAELKGTMRSMVLIAQKQGWKTLFSGLSINYIKVVPSAAIGFTVYDTMKSYLRVPSRDEVD