AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66736


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66736.t1 MTR_2g086680 68.605 172 52 2 1 170 1 172 1.20e-84 246
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66736.t1 AT5G22430 47.134 157 81 2 15 170 17 172 5.92e-45 146
MS.gene66736.t1 AT2G27385 45.181 166 82 3 5 170 6 162 2.97e-41 136
MS.gene66736.t1 AT2G27385 45.181 166 82 3 5 170 17 173 4.47e-41 136
MS.gene66736.t1 AT2G27385 45.181 166 82 3 5 170 17 173 4.47e-41 136

Find 27 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCCTTACAATTTGTGTTT+TGG 0.164797 4.3:-47219977 None:intergenic
CAAGTTTCACAAGTTATTAA+AGG 0.220047 4.3:+47219897 MS.gene66736:CDS
AACTGTTTGGCAAAACTTCT+TGG 0.296592 4.3:+47219840 MS.gene66736:CDS
ATCAGCATGTGAAATTGTTC+AGG 0.312927 4.3:+47218698 MS.gene66736:CDS
CAAAGGAAGATCAATAGTTT+TGG 0.318080 4.3:-47220018 None:intergenic
TCTTCCTTTGCCTCCAGAAT+GGG 0.330802 4.3:+47220031 MS.gene66736:CDS
CTAGGTGCCAAACCCCATTC+TGG 0.349471 4.3:-47220044 None:intergenic
TGTAAGGCTGCAAAACCTAT+TGG 0.353116 4.3:+47219990 MS.gene66736:CDS
CTTTAATAACTTGTGAAACT+TGG 0.355048 4.3:-47219896 None:intergenic
AATTAAGGTATTCCAATGAT+AGG 0.380107 4.3:-47220089 None:intergenic
ACTCATAGCTACGATTTATC+TGG 0.396323 4.3:+47218744 MS.gene66736:CDS
TTTGCCTCCAGAATGGGGTT+TGG 0.402516 4.3:+47220037 MS.gene66736:CDS
ATCTTCCTTTGCCTCCAGAA+TGG 0.404991 4.3:+47220030 MS.gene66736:CDS
CTTCTGTAGTTGTCATAGCT+AGG 0.461561 4.3:-47219764 None:intergenic
GAACAATTTCACATGCTGAT+AGG 0.462045 4.3:-47218695 None:intergenic
TGAGGGAAGGTTAACCTTGA+AGG 0.474424 4.3:-47219796 None:intergenic
TGTTTGGCAAAACTTCTTGG+TGG 0.491974 4.3:+47219843 MS.gene66736:CDS
CTTCCTTTGCCTCCAGAATG+GGG 0.503336 4.3:+47220032 MS.gene66736:CDS
ATTCCAATGATAGGAACAAA+AGG 0.504181 4.3:-47220080 None:intergenic
AGAAGAAGATGGCTCCTTCA+AGG 0.515472 4.3:+47219782 MS.gene66736:CDS
CATGATGTCAAGAAACTAAG+AGG 0.549180 4.3:-47219951 None:intergenic
AAACCCCATTCTGGAGGCAA+AGG 0.556721 4.3:-47220035 None:intergenic
TCAGCATGTGAAATTGTTCA+GGG 0.561309 4.3:+47218699 MS.gene66736:CDS
AAAGGTACATAATAACTACT+AGG 0.564274 4.3:-47220062 None:intergenic
ATGACAACTACAGAAGAAGA+TGG 0.604128 4.3:+47219771 MS.gene66736:CDS
AAGGTCACAGTGAAATGTGA+AGG 0.615631 4.3:+47219732 MS.gene66736:CDS
GGTGCCAAACCCCATTCTGG+AGG 0.664114 4.3:-47220041 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATAGTTTATTTATTAATA+GGG + chr4.3:47218935-47218954 MS.gene66736:intron 10.0%
!! TCAATAGTTTATTTATTAAT+AGG + chr4.3:47218934-47218953 MS.gene66736:intron 10.0%
!!! AAGATAAATTTTCTATTTAT+GGG + chr4.3:47219170-47219189 MS.gene66736:intron 10.0%
!!! AAAATTAGTAACTTTTACAT+TGG + chr4.3:47219102-47219121 MS.gene66736:intron 15.0%
!!! AATGTAAAAGTTACTAATTT+TGG - chr4.3:47219103-47219122 None:intergenic 15.0%
!!! CAAGATAAATTTTCTATTTA+TGG + chr4.3:47219169-47219188 MS.gene66736:intron 15.0%
!! AATAGTAGACATATGTATTA+TGG + chr4.3:47218851-47218870 MS.gene66736:intron 20.0%
!! AATCATGTATGTTTCTTTAA+AGG - chr4.3:47219235-47219254 None:intergenic 20.0%
!! ATCATGTATGTTTCTTTAAA+GGG - chr4.3:47219234-47219253 None:intergenic 20.0%
!! GAACATTTAATTAAACTCAA+AGG - chr4.3:47218799-47218818 None:intergenic 20.0%
!! TACAAGATGCATATAATATA+TGG - chr4.3:47219674-47219693 None:intergenic 20.0%
!! TTTGTTAAGAAATACACATT+TGG + chr4.3:47219287-47219306 MS.gene66736:intron 20.0%
!!! TAATTTTTTAGAAAGAGTGT+GGG + chr4.3:47219529-47219548 MS.gene66736:intron 20.0%
!!! TCAATGATTTTCAGATATTA+AGG + chr4.3:47219713-47219732 MS.gene66736:intron 20.0%
!!! TGTGTATTTATGACTTTTAT+AGG + chr4.3:47219129-47219148 MS.gene66736:intron 20.0%
! AAGGCCTTTACAAAAAATTA+CGG - chr4.3:47219380-47219399 None:intergenic 25.0%
! CAAGTTTCACAAGTTATTAA+AGG + chr4.3:47219897-47219916 MS.gene66736:CDS 25.0%
! TTAAACTCAAAGGCAAATTA+TGG - chr4.3:47218789-47218808 None:intergenic 25.0%
!! AAAGGTACATAATAACTACT+AGG - chr4.3:47220065-47220084 None:intergenic 25.0%
!! CAATTTTACCTCTTAAAGAA+GGG + chr4.3:47219039-47219058 MS.gene66736:intron 25.0%
!! CTTTAATAACTTGTGAAACT+TGG - chr4.3:47219899-47219918 None:intergenic 25.0%
!!! ACAATAGTTTTTGTGTTGTT+TGG - chr4.3:47219267-47219286 None:intergenic 25.0%
!!! CAATAGTTTTTGTGTTGTTT+GGG - chr4.3:47219266-47219285 None:intergenic 25.0%
!!! CTATTTTTTAATGTCCCTAT+GGG + chr4.3:47219014-47219033 MS.gene66736:intron 25.0%
!!! GTAATTTTTTAGAAAGAGTG+TGG + chr4.3:47219528-47219547 MS.gene66736:intron 25.0%
!!! TAAAAAATAGGAGCTTTGTT+TGG - chr4.3:47219004-47219023 None:intergenic 25.0%
AAAAACTTCCATGAACTGTT+TGG + chr4.3:47219827-47219846 MS.gene66736:CDS 30.0%
AGGCCTTTACAAAAAATTAC+GGG - chr4.3:47219379-47219398 None:intergenic 30.0%
ATTCCAATGATAGGAACAAA+AGG - chr4.3:47220083-47220102 None:intergenic 30.0%
CCATAGGGACATTAAAAAAT+AGG - chr4.3:47219016-47219035 None:intergenic 30.0%
CTAAAAAATTACATGTGTCC+CGG - chr4.3:47219520-47219539 None:intergenic 30.0%
TTGTTTGGAGATTAAAGCTA+GGG - chr4.3:47218989-47219008 None:intergenic 30.0%
TTTATACCAGATGTCTCTTT+CGG - chr4.3:47218965-47218984 None:intergenic 30.0%
TTTGTTTGGAGATTAAAGCT+AGG - chr4.3:47218990-47219009 None:intergenic 30.0%
! AGCCTTACAATTTGTGTTTT+GGG - chr4.3:47219979-47219998 None:intergenic 30.0%
! CAAAGGAAGATCAATAGTTT+TGG - chr4.3:47220021-47220040 None:intergenic 30.0%
! GCAATTTTACCTCTTAAAGA+AGG + chr4.3:47219038-47219057 MS.gene66736:intron 30.0%
!! TTTTTGGCATAAAGCTGAAT+TGG - chr4.3:47219870-47219889 None:intergenic 30.0%
!!! ATAGTTTTGGATGAACCAAT+AGG - chr4.3:47220008-47220027 None:intergenic 30.0%
!!! CCTATTTTTTAATGTCCCTA+TGG + chr4.3:47219013-47219032 MS.gene66736:intron 30.0%
!!! TGAAACTTGGTTTTGCTTTT+TGG - chr4.3:47219886-47219905 None:intergenic 30.0%
AAACGTCTCAAAGTATACTC+CGG + chr4.3:47219498-47219517 MS.gene66736:intron 35.0%
AACTGTTTGGCAAAACTTCT+TGG + chr4.3:47219840-47219859 MS.gene66736:CDS 35.0%
ACTCATAGCTACGATTTATC+TGG + chr4.3:47218744-47218763 MS.gene66736:CDS 35.0%
ATCAGCATGTGAAATTGTTC+AGG + chr4.3:47218698-47218717 MS.gene66736:CDS 35.0%
ATGACAACTACAGAAGAAGA+TGG + chr4.3:47219771-47219790 MS.gene66736:CDS 35.0%
ATGCTTAAACAATGCCCTAA+GGG - chr4.3:47219080-47219099 None:intergenic 35.0%
CATCCACCATTTGATACATA+AGG - chr4.3:47219601-47219620 None:intergenic 35.0%
CATGATGTCAAGAAACTAAG+AGG - chr4.3:47219954-47219973 None:intergenic 35.0%
CCTTTACAAAAAATTACGGG+AGG - chr4.3:47219376-47219395 None:intergenic 35.0%
CTTAAGGTTCCAAGAATGTT+GGG + chr4.3:47219571-47219590 MS.gene66736:intron 35.0%
GAACAATTTCACATGCTGAT+AGG - chr4.3:47218698-47218717 None:intergenic 35.0%
GTATACTTCTAGACACATGT+TGG - chr4.3:47219449-47219468 None:intergenic 35.0%
TACCTTGGAGATTTCACTTA+TGG + chr4.3:47219408-47219427 MS.gene66736:intron 35.0%
TAGCATTTCCCTTCTTTAAG+AGG - chr4.3:47219050-47219069 None:intergenic 35.0%
TCAGCATGTGAAATTGTTCA+GGG + chr4.3:47218699-47218718 MS.gene66736:CDS 35.0%
TTCCTTAAACAATGCCCTTA+GGG + chr4.3:47219193-47219212 MS.gene66736:intron 35.0%
TTGAAGAACAATGAGAGTCA+TGG - chr4.3:47219697-47219716 None:intergenic 35.0%
! AAGTTTTGCCAAACAGTTCA+TGG - chr4.3:47219838-47219857 None:intergenic 35.0%
! CAGCCTTACAATTTGTGTTT+TGG - chr4.3:47219980-47219999 None:intergenic 35.0%
! GAAGTTTTTGTGTTGTCTGA+GGG - chr4.3:47219816-47219835 None:intergenic 35.0%
! GCCTTACAATTTGTGTTTTG+GGG - chr4.3:47219978-47219997 None:intergenic 35.0%
! GTACCTTTTGTTCCTATCAT+TGG + chr4.3:47220077-47220096 MS.gene66736:CDS 35.0%
! TCTCCAAGGTATTATCAACA+AGG - chr4.3:47219399-47219418 None:intergenic 35.0%
!! CCTTACAATTTGTGTTTTGG+GGG - chr4.3:47219977-47219996 None:intergenic 35.0%
!!! AAGCTGTGTTTTTTCCCATA+TGG + chr4.3:47219319-47219338 MS.gene66736:intron 35.0%
!!! AGCTGTGTTTTTTCCCATAT+GGG + chr4.3:47219320-47219339 MS.gene66736:intron 35.0%
!!! CCTCCCGTAATTTTTTGTAA+AGG + chr4.3:47219373-47219392 MS.gene66736:intron 35.0%
AAATGCTAACAAGTGCCCTT+AGG + chr4.3:47219062-47219081 MS.gene66736:intron 40.0%
AACGTCTCAAAGTATACTCC+GGG + chr4.3:47219499-47219518 MS.gene66736:intron 40.0%
AAGGTCACAGTGAAATGTGA+AGG + chr4.3:47219732-47219751 MS.gene66736:CDS 40.0%
AATGCTAACAAGTGCCCTTA+GGG + chr4.3:47219063-47219082 MS.gene66736:intron 40.0%
ACTTCTAGACACATGTTGGT+TGG - chr4.3:47219445-47219464 None:intergenic 40.0%
AGAGGTAAAATTGCTCCCAT+AGG - chr4.3:47219032-47219051 None:intergenic 40.0%
CACCATTTGATACATAAGGC+CGG - chr4.3:47219597-47219616 None:intergenic 40.0%
CCAACATTCTTGGAACCTTA+AGG - chr4.3:47219573-47219592 None:intergenic 40.0%
CCCCCAAAACACAAATTGTA+AGG + chr4.3:47219974-47219993 MS.gene66736:CDS 40.0%
CCTTAAGGTTCCAAGAATGT+TGG + chr4.3:47219570-47219589 MS.gene66736:intron 40.0%
CTCCATAAGTGAAATCTCCA+AGG - chr4.3:47219413-47219432 None:intergenic 40.0%
CTTCTGTAGTTGTCATAGCT+AGG - chr4.3:47219767-47219786 None:intergenic 40.0%
CTTTGTCACTCAGAAGAGTT+CGG - chr4.3:47219351-47219370 None:intergenic 40.0%
GAGGTAAAATTGCTCCCATA+GGG - chr4.3:47219031-47219050 None:intergenic 40.0%
GATGCTTAAACAATGCCCTA+AGG - chr4.3:47219081-47219100 None:intergenic 40.0%
GTTCCTTAAACAATGCCCTT+AGG + chr4.3:47219192-47219211 MS.gene66736:intron 40.0%
TGTAAGGCTGCAAAACCTAT+TGG + chr4.3:47219990-47220009 MS.gene66736:CDS 40.0%
TGTTTGGCAAAACTTCTTGG+TGG + chr4.3:47219843-47219862 MS.gene66736:CDS 40.0%
TTTGCTAACAAGTGCCCTAA+GGG - chr4.3:47219210-47219229 None:intergenic 40.0%
! CTTCTAGACACATGTTGGTT+GGG - chr4.3:47219444-47219463 None:intergenic 40.0%
! GGAAGTTTTTGTGTTGTCTG+AGG - chr4.3:47219817-47219836 None:intergenic 40.0%
! TTTTGCTAACAAGTGCCCTA+AGG - chr4.3:47219211-47219230 None:intergenic 40.0%
! TTTTTGTGTTGTCTGAGGGA+AGG - chr4.3:47219812-47219831 None:intergenic 40.0%
!! AGGCCTTGTTGATAATACCT+TGG + chr4.3:47219393-47219412 MS.gene66736:intron 40.0%
AAAGAAGCTCGCTTGCCTTA+AGG + chr4.3:47219555-47219574 MS.gene66736:intron 45.0%
AGAAGAAGATGGCTCCTTCA+AGG + chr4.3:47219782-47219801 MS.gene66736:CDS 45.0%
AGAGTTCGGATGTACCCATA+TGG - chr4.3:47219337-47219356 None:intergenic 45.0%
ATCTTCCTTTGCCTCCAGAA+TGG + chr4.3:47220030-47220049 MS.gene66736:CDS 45.0%
GAGTTCGGATGTACCCATAT+GGG - chr4.3:47219336-47219355 None:intergenic 45.0%
TACCAGATGTCTCTTTCGGT+CGG - chr4.3:47218961-47218980 None:intergenic 45.0%
TCTTCCTTTGCCTCCAGAAT+GGG + chr4.3:47220031-47220050 MS.gene66736:CDS 45.0%
TGAGGGAAGGTTAACCTTGA+AGG - chr4.3:47219799-47219818 None:intergenic 45.0%
! CGGCCTTATGTATCAAATGG+TGG + chr4.3:47219595-47219614 MS.gene66736:intron 45.0%
!! TGCCCTAAGGGCATTGTTTA+AGG - chr4.3:47219198-47219217 None:intergenic 45.0%
AGGTTCCAAGAATGTTGGGC+CGG + chr4.3:47219575-47219594 MS.gene66736:intron 50.0%
CTTCCTTTGCCTCCAGAATG+GGG + chr4.3:47220032-47220051 MS.gene66736:CDS 50.0%
TTTGCCTCCAGAATGGGGTT+TGG + chr4.3:47220037-47220056 MS.gene66736:CDS 50.0%
! AAACCCCATTCTGGAGGCAA+AGG - chr4.3:47220038-47220057 None:intergenic 50.0%
! GGCCGGCCTTATGTATCAAA+TGG + chr4.3:47219592-47219611 MS.gene66736:intron 50.0%
CTAGGTGCCAAACCCCATTC+TGG - chr4.3:47220047-47220066 None:intergenic 55.0%
TAAGGCCGGCCCAACATTCT+TGG - chr4.3:47219583-47219602 None:intergenic 55.0%
GGCCGACCGAAAGAGACATC+TGG + chr4.3:47218956-47218975 MS.gene66736:intron 60.0%
GGTGCCAAACCCCATTCTGG+AGG - chr4.3:47220044-47220063 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 47218636 47220109 47218636 ID=MS.gene66736
chr4.3 mRNA 47218636 47220109 47218636 ID=MS.gene66736.t1;Parent=MS.gene66736
chr4.3 exon 47218636 47218765 47218636 ID=MS.gene66736.t1.exon1;Parent=MS.gene66736.t1
chr4.3 CDS 47218636 47218765 47218636 ID=cds.MS.gene66736.t1;Parent=MS.gene66736.t1
chr4.3 exon 47219727 47220109 47219727 ID=MS.gene66736.t1.exon2;Parent=MS.gene66736.t1
chr4.3 CDS 47219727 47220109 47219727 ID=cds.MS.gene66736.t1;Parent=MS.gene66736.t1
Gene Sequence

>MS.gene66736

ATGGCATATGTTCATGTTATCACAGCTCTTCTCTTTGCATTAGTTCTTGTTAGAATTGACCTATCAGCATGTGAAATTGTTCAGGGTAAAGTCTCATGTGTTGACTGCACTCATAGCTACGATTTATCTGGTATGTGCTAACTCACTATGCCATAATTTGCCTTTGAGTTTAATTAAATGTTCATTACTTTGTTTCTATAGTTTTACTCAGCTTAAATAGTAGACATATGTATTATGGATTTGACTATGAGAATAATAAAATACATTAACACGTTTATGATTAGTTATACATGTGAAATCAATAGTTTATTTATTAATAGGGCCGACCGAAAGAGACATCTGGTATAAAACCCTAGCTTTAATCTCCAAACAAAGCTCCTATTTTTTAATGTCCCTATGGGAGCAATTTTACCTCTTAAAGAAGGGAAATGCTAACAAGTGCCCTTAGGGCATTGTTTAAGCATCCAAAATTAGTAACTTTTACATTGGAAATTGTGTATTTATGACTTTTATAGGTGTTTGACTTGTATTTTCAAGATAAATTTTCTATTTATGGGTTCCTTAAACAATGCCCTTAGGGCACTTGTTAGCAAAACCCTTTAAAGAAACATACATGATTTTAGAACACCCAAACAACACAAAAACTATTGTTTTGTTAAGAAATACACATTTGGAAAACACGTAAGCTGTGTTTTTTCCCATATGGGTACATCCGAACTCTTCTGAGTGACAAAGTTCCTCCCGTAATTTTTTGTAAAGGCCTTGTTGATAATACCTTGGAGATTTCACTTATGGAGCTTTTTTTCCCAACCAACATGTGTCTAGAAGTATACTTCTGAAAAGAAACAAAAAAAAAAAAAAAAAACGTCTCAAAGTATACTCCGGGACACATGTAATTTTTTAGAAAGAGTGTGGGAAAAAAGAAGCTCGCTTGCCTTAAGGTTCCAAGAATGTTGGGCCGGCCTTATGTATCAAATGGTGGATGTTACTCATTTTACACTTCAAAATAAGTTCTTAACTTTAGAAGAACTAGATCCATATATTATATGCATCTTGTACCATGACTCTCATTGTTCTTCAATGATTTTCAGATATTAAGGTCACAGTGAAATGTGAAGGTGTTAAAAACCTAGCTATGACAACTACAGAAGAAGATGGCTCCTTCAAGGTTAACCTTCCCTCAGACAACACAAAAACTTCCATGAACTGTTTGGCAAAACTTCTTGGTGGACCAATTCAGCTTTATGCCAAAAAGCAAAACCAAGTTTCACAAGTTATTAAAGGAAAAGAAGAAAACAGCTACACAATCTCAACTCCTCTTAGTTTCTTGACATCATGCCCCCAAAACACAAATTGTAAGGCTGCAAAACCTATTGGTTCATCCAAAACTATTGATCTTCCTTTGCCTCCAGAATGGGGTTTGGCACCTAGTAGTTATTATGTACCTTTTGTTCCTATCATTGGAATACCTTAA

Protein sequence

>MS.gene66736.t1

MAYVHVITALLFALVLVRIDLSACEIVQGKVSCVDCTHSYDLSDIKVTVKCEGVKNLAMTTTEEDGSFKVNLPSDNTKTSMNCLAKLLGGPIQLYAKKQNQVSQVIKGKEENSYTISTPLSFLTSCPQNTNCKAAKPIGSSKTIDLPLPPEWGLAPSSYYVPFVPIIGIP