AlfalfaGEDB | MS.gene66969

M. sativa cultivar XinJiangDaYe / MS.gene66969

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0006355 GO:0008150 GO:0008270 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0043167 GO:0043169 GO:0043565 GO:0046872 GO:0046914 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00320.30

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66969.t1	XP_003619010.2	86.5	200	15	2	1	200	74	261	1.10E-87	332.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66969.t1	O49743	80.9	68	13	0	130	197	157	224	1.7e-28	127.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66969.t1	G7KQ55	86.5	200	15	2	1	200	74	261	7.9e-88	332.8

TFs/TRs:

Gene ID	Type	Classification
MS.gene66969.t1	TF	C2C2-GATA

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049195	MS.gene66969	0.811623	6.38E-51	-1.69E-46
MS.gene049260	MS.gene66969	0.832626	8.49E-56	-1.69E-46
MS.gene050442	MS.gene66969	0.84028	9.56E-58	-1.69E-46
MS.gene050750	MS.gene66969	0.807865	4.10E-50	-1.69E-46
MS.gene050787	MS.gene66969	0.840632	7.73E-58	-1.69E-46
MS.gene050815	MS.gene66969	0.81682	4.54E-52	-1.69E-46
MS.gene051265	MS.gene66969	0.823327	1.47E-53	-1.69E-46
MS.gene051344	MS.gene66969	0.813439	2.56E-51	-1.69E-46
MS.gene051860	MS.gene66969	0.837403	5.31E-57	-1.69E-46
MS.gene052190	MS.gene66969	0.806872	6.66E-50	-1.69E-46
MS.gene053054	MS.gene66969	-0.801147	1.03E-48	-1.69E-46
MS.gene053166	MS.gene66969	0.802622	5.14E-49	-1.69E-46
MS.gene053836	MS.gene66969	0.815554	8.71E-52	-1.69E-46
MS.gene054184	MS.gene66969	0.806638	7.46E-50	-1.69E-46
MS.gene054185	MS.gene66969	0.804455	2.14E-49	-1.69E-46
MS.gene054433	MS.gene66969	0.801381	9.25E-49	-1.69E-46
MS.gene054473	MS.gene66969	0.837558	4.84E-57	-1.69E-46
MS.gene054542	MS.gene66969	0.805186	1.51E-49	-1.69E-46
MS.gene054713	MS.gene66969	0.846572	2.00E-59	-1.69E-46
MS.gene055356	MS.gene66969	0.856439	3.22E-62	-1.69E-46
MS.gene055358	MS.gene66969	0.881853	1.70E-70	-1.69E-46
MS.gene055608	MS.gene66969	0.80949	1.84E-50	-1.69E-46
MS.gene057639	MS.gene66969	0.839448	1.57E-57	-1.69E-46
MS.gene057693	MS.gene66969	0.808202	3.48E-50	-1.69E-46
MS.gene058168	MS.gene66969	0.807876	4.08E-50	-1.69E-46
MS.gene059607	MS.gene66969	0.810975	8.82E-51	-1.69E-46
MS.gene059609	MS.gene66969	0.818001	2.46E-52	-1.69E-46
MS.gene059789	MS.gene66969	0.800003	1.77E-48	-1.69E-46
MS.gene060137	MS.gene66969	-0.817208	3.71E-52	-1.69E-46
MS.gene060322	MS.gene66969	0.821294	4.36E-53	-1.69E-46
MS.gene060572	MS.gene66969	0.800371	1.49E-48	-1.69E-46
MS.gene060683	MS.gene66969	0.800244	1.58E-48	-1.69E-46
MS.gene060802	MS.gene66969	0.819587	1.07E-52	-1.69E-46
MS.gene060803	MS.gene66969	0.852366	4.84E-61	-1.69E-46
MS.gene061040	MS.gene66969	-0.817688	2.90E-52	-1.69E-46
MS.gene061114	MS.gene66969	-0.832792	7.72E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66969.t1	MTR_6g032830	91.000	200	6	2	1	200	74	261	7.05e-131	368
MS.gene66969.t1	MTR_7g085410	57.561	205	49	7	1	199	84	256	3.59e-68	209
MS.gene66969.t1	MTR_1g094930	80.000	65	13	0	131	195	147	211	8.70e-35	123
MS.gene66969.t1	MTR_4g071590	81.538	65	12	0	131	195	211	275	1.06e-34	124
MS.gene66969.t1	MTR_4g471590	81.538	65	12	0	131	195	211	275	1.06e-34	124
MS.gene66969.t1	MTR_1g094930	80.000	65	13	0	131	195	242	306	3.15e-34	124
MS.gene66969.t1	MTR_1g006650	77.612	67	15	0	131	197	234	300	6.68e-34	123
MS.gene66969.t1	MTR_2g094600	77.143	70	16	0	131	200	216	285	7.07e-34	122
MS.gene66969.t1	MTR_8g076340	73.333	75	18	1	121	195	210	282	1.05e-33	122
MS.gene66969.t1	MTR_8g013950	73.913	69	18	0	129	197	224	292	1.99e-33	122
MS.gene66969.t1	MTR_1g116010	70.270	74	22	0	123	196	156	229	3.31e-33	119
MS.gene66969.t1	MTR_3g109760	68.750	80	20	1	118	197	199	273	4.10e-33	120
MS.gene66969.t1	MTR_3g117380	78.462	65	14	0	131	195	229	293	1.59e-32	119
MS.gene66969.t1	MTR_1g046680	76.471	68	16	0	130	197	171	238	2.51e-32	117
MS.gene66969.t1	MTR_5g021340	80.000	65	13	0	131	195	300	364	2.55e-32	120
MS.gene66969.t1	MTR_5g007600	72.059	68	19	0	130	197	258	325	3.68e-31	116
MS.gene66969.t1	MTR_5g007600	68.657	67	21	0	130	196	156	222	6.16e-29	110
MS.gene66969.t1	MTR_4g058910	76.923	65	15	0	131	195	314	378	3.91e-31	117
MS.gene66969.t1	MTR_7g446110	57.576	66	28	0	127	192	183	248	3.96e-21	87.8
MS.gene66969.t1	MTR_7g445950	56.452	62	27	0	131	192	120	181	3.57e-19	81.3
MS.gene66969.t1	MTR_7g445980	58.730	63	25	1	131	192	33	95	8.40e-19	77.8
MS.gene66969.t1	MTR_7g446070	54.839	62	28	0	131	192	132	193	1.12e-18	80.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66969.t1	AT4G32890	45.652	184	44	8	14	197	136	263	1.33e-37	132
MS.gene66969.t1	AT3G60530	79.710	69	14	0	129	197	156	224	1.82e-36	127
MS.gene66969.t1	AT5G25830	38.776	196	89	5	2	197	121	285	5.18e-36	129
MS.gene66969.t1	AT2G45050	79.104	67	14	0	131	197	179	245	4.42e-35	124
MS.gene66969.t1	AT3G24050	75.000	68	17	0	130	197	193	260	1.78e-34	123
MS.gene66969.t1	AT3G54810	67.470	83	26	1	113	195	212	293	2.74e-33	121
MS.gene66969.t1	AT3G54810	67.470	83	26	1	113	195	212	293	2.74e-33	121
MS.gene66969.t1	AT5G66320	80.000	65	13	0	131	195	249	313	4.11e-33	121
MS.gene66969.t1	AT5G66320	80.000	65	13	0	131	195	249	313	4.11e-33	121
MS.gene66969.t1	AT3G51080	81.538	65	12	0	131	195	221	285	4.43e-33	120
MS.gene66969.t1	AT4G36240	72.464	69	19	0	131	199	164	232	5.68e-33	118
MS.gene66969.t1	AT1G08010	71.212	66	19	0	130	195	219	284	1.49e-32	119
MS.gene66969.t1	AT1G08010	71.212	66	19	0	130	195	219	284	1.49e-32	119
MS.gene66969.t1	AT1G08010	71.212	66	19	0	130	195	219	284	1.49e-32	119
MS.gene66969.t1	AT4G34680	73.846	65	17	0	131	195	180	244	1.82e-31	115
MS.gene66969.t1	AT4G34680	73.846	65	17	0	131	195	180	244	1.82e-31	115
MS.gene66969.t1	AT1G08000	72.727	66	18	0	130	195	217	282	2.31e-31	116
MS.gene66969.t1	AT1G08000	72.727	66	18	0	130	195	217	282	2.31e-31	116
MS.gene66969.t1	AT2G28340	66.154	65	22	0	133	197	193	257	1.57e-27	105
MS.gene66969.t1	AT3G45170	61.538	65	25	0	133	197	117	181	3.85e-26	100
MS.gene66969.t1	AT5G49300	46.296	54	22	1	113	166	25	71	5.84e-11	58.5

Find 34 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTGTTGAAACAAGCTTAT+TGG	0.158008	6.4:+4035581	MS.gene66969:CDS
CAGCTACTAGTACCAAAATT+TGG	0.280451	6.4:+4035512	MS.gene66969:CDS
ATGTGAATGTGACCAAATTT+TGG	0.288556	6.4:-4035524	None:intergenic
CATGCATTGCATAGTGTCTT+TGG	0.309772	6.4:-4035877	None:intergenic
ATGGTCCTGCCCTCCATTGT+GGG	0.318440	6.4:-4035849	None:intergenic
TGAGTGCACCTTCTTGCATT+TGG	0.340927	6.4:-4035808	None:intergenic
ACAATGGAGGGCAGGACCAT+TGG	0.400380	6.4:+4035852	MS.gene66969:CDS
AATGGTCCTGCCCTCCATTG+TGG	0.428822	6.4:-4035850	None:intergenic
GAGAATGCAATCTATTAAGT+AGG	0.465726	6.4:+4036008	MS.gene66969:CDS
CAATGGAGGGCAGGACCATT+GGG	0.466961	6.4:+4035853	MS.gene66969:CDS
CGGAGCAATGAGTTCGCTGT+CGG	0.489222	6.4:-4035608	None:intergenic
GTAGGTTGTTGCCAGAGTAT+AGG	0.492955	6.4:+4035920	MS.gene66969:CDS
CATAGTGTCTTTGGTCCCAA+TGG	0.499888	6.4:-4035868	None:intergenic
TGCAAGTAGCTAACAAAAGT+AGG	0.538933	6.4:-4035955	None:intergenic
CTCTTCGCTGGCCTATACTC+TGG	0.539921	6.4:-4035931	None:intergenic
AGGACCCCACAATGGAGGGC+AGG	0.548944	6.4:+4035844	MS.gene66969:CDS
TGGTCCTGCCCTCCATTGTG+GGG	0.559124	6.4:-4035848	None:intergenic
AACATATTCCAAATGCAAGA+AGG	0.563153	6.4:+4035800	MS.gene66969:CDS
AAGCTTGTTTCAACAAAGGA+GGG	0.570788	6.4:-4035576	None:intergenic
AGAATGCAATCTATTAAGTA+GGG	0.572327	6.4:+4036009	MS.gene66969:CDS
TAAGCTTGTTTCAACAAAGG+AGG	0.573541	6.4:-4035577	None:intergenic
CATTCACATCTTATAAGTGA+TGG	0.584037	6.4:+4035538	MS.gene66969:CDS
TGTGGTGTGAGGTACAAGTC+TGG	0.585109	6.4:+4035898	MS.gene66969:CDS
CAATAAGCTTGTTTCAACAA+AGG	0.588114	6.4:-4035580	None:intergenic
AAAGTCTCTGCAGCTGCGTG+CGG	0.588531	6.4:+4035643	MS.gene66969:CDS
ACAAAGGACCCCACAATGGA+GGG	0.595325	6.4:+4035840	MS.gene66969:CDS
GTGTGAGGTACAAGTCTGGT+AGG	0.597780	6.4:+4035902	MS.gene66969:CDS
GCACTCATTGTTTGTCACAA+AGG	0.603068	6.4:+4035824	MS.gene66969:CDS
CACAAAGGACCCCACAATGG+AGG	0.605327	6.4:+4035839	MS.gene66969:CDS
ACAAAAGTAGGACTCTTCGC+TGG	0.624466	6.4:-4035943	None:intergenic
TGATGTTAATGATGATGATG+AGG	0.646926	6.4:+4035777	MS.gene66969:CDS
TGTCACAAAGGACCCCACAA+TGG	0.681006	6.4:+4035836	MS.gene66969:CDS
TATGCAATGCATGTGGTGTG+AGG	0.734829	6.4:+4035887	MS.gene66969:CDS
AAGACACTATGCAATGCATG+TGG	0.764250	6.4:+4035880	MS.gene66969:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAAGATTGATTTTGTTGTTG+AGG	-	chr6.4:4035439-4035458	None:intergenic	25.0%
	AACATATTCCAAATGCAAGA+AGG	+	chr6.4:4035800-4035819	MS.gene66969:CDS	30.0%
	CATTCACATCTTATAAGTGA+TGG	+	chr6.4:4035538-4035557	MS.gene66969:CDS	30.0%
	CTTTGTTGAAACAAGCTTAT+TGG	+	chr6.4:4035581-4035600	MS.gene66969:CDS	30.0%
!	ATGTGAATGTGACCAAATTT+TGG	-	chr6.4:4035527-4035546	None:intergenic	30.0%
!	CAATAAGCTTGTTTCAACAA+AGG	-	chr6.4:4035583-4035602	None:intergenic	30.0%
!	TGATGTTAATGATGATGATG+AGG	+	chr6.4:4035777-4035796	MS.gene66969:CDS	30.0%
!!	GAGACTTTTTGCTCATTTTT+CGG	-	chr6.4:4035631-4035650	None:intergenic	30.0%
!!!	TTGCAAAATTTTGTTGTTCC+TGG	+	chr6.4:4035460-4035479	MS.gene66969:CDS	30.0%
	ATCAGACAGAAGCAAAAAGA+AGG	+	chr6.4:4035668-4035687	MS.gene66969:CDS	35.0%
	CAGCTACTAGTACCAAAATT+TGG	+	chr6.4:4035512-4035531	MS.gene66969:CDS	35.0%
	TCAGACAGAAGCAAAAAGAA+GGG	+	chr6.4:4035669-4035688	MS.gene66969:CDS	35.0%
	TGCAAGTAGCTAACAAAAGT+AGG	-	chr6.4:4035958-4035977	None:intergenic	35.0%
!	AAGCTTGTTTCAACAAAGGA+GGG	-	chr6.4:4035579-4035598	None:intergenic	35.0%
!	GAAAGAGAAAGAGAGTTTTG+AGG	+	chr6.4:4035714-4035733	MS.gene66969:CDS	35.0%
!	TAAGCTTGTTTCAACAAAGG+AGG	-	chr6.4:4035580-4035599	None:intergenic	35.0%
	AAAAAGAAGGGTGAAGAGAG+TGG	+	chr6.4:4035681-4035700	MS.gene66969:CDS	40.0%
	AAGACACTATGCAATGCATG+TGG	+	chr6.4:4035880-4035899	MS.gene66969:CDS	40.0%
	CATGCATTGCATAGTGTCTT+TGG	-	chr6.4:4035880-4035899	None:intergenic	40.0%
	GCACTCATTGTTTGTCACAA+AGG	+	chr6.4:4035824-4035843	MS.gene66969:CDS	40.0%
	GGTGAAGAGAGTGGTAAAAA+TGG	+	chr6.4:4035690-4035709	MS.gene66969:CDS	40.0%
!!	CTCTTACTTCTTGCTTTTCC+AGG	-	chr6.4:4035481-4035500	None:intergenic	40.0%
!!!	GAGAAAGAGAGTTTTGAGGT+TGG	+	chr6.4:4035718-4035737	MS.gene66969:CDS	40.0%
	ACAAAAGTAGGACTCTTCGC+TGG	-	chr6.4:4035946-4035965	None:intergenic	45.0%
	CATAGTGTCTTTGGTCCCAA+TGG	-	chr6.4:4035871-4035890	None:intergenic	45.0%
	GTAGGTTGTTGCCAGAGTAT+AGG	+	chr6.4:4035920-4035939	MS.gene66969:CDS	45.0%
	TATGCAATGCATGTGGTGTG+AGG	+	chr6.4:4035887-4035906	MS.gene66969:CDS	45.0%
	TGAGTGCACCTTCTTGCATT+TGG	-	chr6.4:4035811-4035830	None:intergenic	45.0%
	ACAAAGGACCCCACAATGGA+GGG	+	chr6.4:4035840-4035859	MS.gene66969:CDS	50.0%
	GTGTGAGGTACAAGTCTGGT+AGG	+	chr6.4:4035902-4035921	MS.gene66969:CDS	50.0%
	TGTCACAAAGGACCCCACAA+TGG	+	chr6.4:4035836-4035855	MS.gene66969:CDS	50.0%
	TGTGGTGTGAGGTACAAGTC+TGG	+	chr6.4:4035898-4035917	MS.gene66969:CDS	50.0%
	AAAGTCTCTGCAGCTGCGTG+CGG	+	chr6.4:4035643-4035662	MS.gene66969:CDS	55.0%
	AATGGTCCTGCCCTCCATTG+TGG	-	chr6.4:4035853-4035872	None:intergenic	55.0%
	ACAATGGAGGGCAGGACCAT+TGG	+	chr6.4:4035852-4035871	MS.gene66969:CDS	55.0%
	ATGGTCCTGCCCTCCATTGT+GGG	-	chr6.4:4035852-4035871	None:intergenic	55.0%
	CAATGGAGGGCAGGACCATT+GGG	+	chr6.4:4035853-4035872	MS.gene66969:CDS	55.0%
	CACAAAGGACCCCACAATGG+AGG	+	chr6.4:4035839-4035858	MS.gene66969:CDS	55.0%
	CGGAGCAATGAGTTCGCTGT+CGG	-	chr6.4:4035611-4035630	None:intergenic	55.0%
!	CTCTTCGCTGGCCTATACTC+TGG	-	chr6.4:4035934-4035953	None:intergenic	55.0%
	TGGTCCTGCCCTCCATTGTG+GGG	-	chr6.4:4035851-4035870	None:intergenic	60.0%
	AGGACCCCACAATGGAGGGC+AGG	+	chr6.4:4035844-4035863	MS.gene66969:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	4035427	4036029	4035427	ID=MS.gene66969
chr6.4	mRNA	4035427	4036029	4035427	ID=MS.gene66969.t1;Parent=MS.gene66969
chr6.4	exon	4035427	4036029	4035427	ID=MS.gene66969.t1.exon1;Parent=MS.gene66969.t1
chr6.4	CDS	4035427	4036029	4035427	ID=cds.MS.gene66969.t1;Parent=MS.gene66969.t1

Gene Sequence

>MS.gene66969

ATGCAAAAACCTCAACAACAAAATCAATCTTATTTGCAAAATTTTGTTGTTCCTGGAAAAGCAAGAAGTAAGAGAAAAAGACTTTCAGCTACTAGTACCAAAATTTGGTCACATTCACATCTTATAAGTGATGGTAATTTGATTTCTGACCCTCCTTTGTTGAAACAAGCTTATTGGTTAGCCGACAGCGAACTCATTGCTCCGAAAAATGAGCAAAAAGTCTCTGCAGCTGCGTGCGGAGATCAGACAGAAGCAAAAAGAAGGGTGAAGAGAGTGGTAAAAATGGAGAAAGAGAAAGAGAGTTTTGAGGTTGGAATTATTCAAGTTAAGAATAGTGAGAATAATAATGATGATGTTAATGATGATGATGAGGAACATATTCCAAATGCAAGAAGGTGCACTCATTGTTTGTCACAAAGGACCCCACAATGGAGGGCAGGACCATTGGGACCAAAGACACTATGCAATGCATGTGGTGTGAGGTACAAGTCTGGTAGGTTGTTGCCAGAGTATAGGCCAGCGAAGAGTCCTACTTTTGTTAGCTACTTGCATTCAAATTCACACAAGAAAGTTCTTGAGATGAGAATGCAATCTATTAAGTAG

Protein sequence

>MS.gene66969.t1

MQKPQQQNQSYLQNFVVPGKARSKRKRLSATSTKIWSHSHLISDGNLISDPPLLKQAYWLADSELIAPKNEQKVSAAACGDQTEAKRRVKRVVKMEKEKESFEVGIIQVKNSENNNDDVNDDDEEHIPNARRCTHCLSQRTPQWRAGPLGPKTLCNACGVRYKSGRLLPEYRPAKSPTFVSYLHSNSHKKVLEMRMQSIK