AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70503


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70503.t1 MTR_3g081500 96.330 218 5 3 1 217 1 216 2.06e-147 409
MS.gene70503.t1 MTR_2g035850 46.392 97 46 3 9 99 13 109 6.82e-18 79.7
MS.gene70503.t1 MTR_4g094698 42.202 109 49 5 7 112 10 107 6.54e-14 67.4
MS.gene70503.t1 MTR_5g063310 62.791 43 16 0 67 109 155 197 2.41e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70503.t1 AT4G39720 31.383 188 116 5 9 191 56 235 9.28e-13 66.2
MS.gene70503.t1 AT4G39720 31.383 188 116 5 9 191 56 235 9.89e-13 66.2
MS.gene70503.t1 AT4G39720 31.383 188 116 5 9 191 56 235 1.09e-12 65.9
MS.gene70503.t1 AT4G39720 31.383 188 116 5 9 191 56 235 1.09e-12 65.9
MS.gene70503.t1 AT3G22160 71.667 60 16 1 53 111 41 100 1.65e-12 64.3
MS.gene70503.t1 AT1G35830 62.222 45 17 0 67 111 100 144 2.13e-11 62.4
MS.gene70503.t1 AT4G15120 80.851 47 9 0 55 101 39 85 4.56e-11 60.1
MS.gene70503.t1 AT5G65170 62.791 43 16 0 67 109 140 182 5.18e-11 61.6

Find 66 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATTCATCATGGTTGATTC+AGG 0.196823 3.1:+67054308 None:intergenic
GTGTAAGGAGGGGTTTGATT+AGG 0.284979 3.1:+67054371 None:intergenic
CTGGCTGAGGTTGGTGAGCT+TGG 0.290535 3.1:+67054287 None:intergenic
TGTAAGGAGGGGTTTGATTA+GGG 0.309491 3.1:+67054372 None:intergenic
TTATCATTGTTTGAAGGTCT+TGG 0.328276 3.1:+67053870 None:intergenic
ACCCTAGCAGGCCTAGGGCT+TGG 0.349369 3.1:-67054074 MS.gene70503:CDS
TTCAGATGGACCCAAGTTGC+TGG 0.349830 3.1:+67054268 None:intergenic
CCTGCTAGGGTTGAGAAATT+AGG 0.351690 3.1:+67054086 None:intergenic
AGGCCTAGGGCTTGGCCCAC+GGG 0.363073 3.1:-67054066 MS.gene70503:CDS
TCTCAAGAAGAAGAAGTTCT+TGG 0.369824 3.1:+67053789 None:intergenic
GTTGTTAGTGATCATATTCA+TGG 0.373249 3.1:-67053831 MS.gene70503:CDS
CAGGCCTAGGGCTTGGCCCA+CGG 0.392030 3.1:-67054067 MS.gene70503:CDS
GCTGCTGCAAAAGGTGCAAT+AGG 0.418378 3.1:+67054128 None:intergenic
GACATCATAGATTGATTCAT+AGG 0.420321 3.1:+67054035 None:intergenic
GATTCATCATGGTTGATTCA+GGG 0.429834 3.1:+67054309 None:intergenic
GTTGTTGTTGAAGCTGTTGT+TGG 0.432601 3.1:+67054000 None:intergenic
AGCCCTTGATCTTCTTCGTA+TGG 0.433702 3.1:+67054229 None:intergenic
TTCTCAACCCTAGCAGGCCT+AGG 0.444711 3.1:-67054080 MS.gene70503:CDS
GGCCAAGCCCTAGGCCTGCT+AGG 0.451902 3.1:+67054072 None:intergenic
AAGCCCGTGGGCCAAGCCCT+AGG 0.457915 3.1:+67054063 None:intergenic
TTAGTAGTAATGGAAGTGTA+AGG 0.460397 3.1:+67054356 None:intergenic
GCCCTTGATCTTCTTCGTAT+GGG 0.464225 3.1:+67054230 None:intergenic
TAGTAATGGAAGTGTAAGGA+GGG 0.468900 3.1:+67054360 None:intergenic
CCTAATTTCTCAACCCTAGC+AGG 0.472155 3.1:-67054086 MS.gene70503:CDS
GATGTGGCTGCTGCTGCAAA+AGG 0.475844 3.1:+67054119 None:intergenic
TGAAGCTGTTGTTGGTAGAA+AGG 0.476011 3.1:+67054008 None:intergenic
CAACACTACAATATGTATAG+TGG 0.476494 3.1:-67053954 MS.gene70503:CDS
ACCAACCTCAGCCAGCAACT+TGG 0.483517 3.1:-67054279 MS.gene70503:CDS
TTCGTATGGGCTTTGACACA+CGG 0.492946 3.1:+67054243 None:intergenic
TCATAGATTGATTCATAGGA+TGG 0.502434 3.1:+67054039 None:intergenic
GCCAAGCCCTAGGCCTGCTA+GGG 0.502498 3.1:+67054073 None:intergenic
AGGGGTGGTGTTAGTAGTAA+TGG 0.506379 3.1:+67054346 None:intergenic
TTATTATTATCATTGTTTGA+AGG 0.509412 3.1:+67053864 None:intergenic
CCCAAGTTGCTGGCTGAGGT+TGG 0.527272 3.1:+67054278 None:intergenic
CCAACCTCAGCCAGCAACTT+GGG 0.528451 3.1:-67054278 MS.gene70503:CDS
GAAGAAGATCAAGGGCTTCG+CGG 0.529169 3.1:-67054223 MS.gene70503:CDS
AGCCCATACGAAGAAGATCA+AGG 0.531159 3.1:-67054232 MS.gene70503:CDS
CACTACTAATTTCCGTGCCA+TGG 0.531867 3.1:-67054171 MS.gene70503:CDS
GTAGTAATGGAAGTGTAAGG+AGG 0.532755 3.1:+67054359 None:intergenic
AGCAACTTGGGTCCATCTGA+AGG 0.541645 3.1:-67054266 MS.gene70503:CDS
CATATTCATGGAGGAGAAGG+TGG 0.544946 3.1:-67053819 MS.gene70503:CDS
TCTCAACCCTAGCAGGCCTA+GGG 0.552947 3.1:-67054079 MS.gene70503:CDS
GATCATATTCATGGAGGAGA+AGG 0.558000 3.1:-67053822 MS.gene70503:CDS
AAAAGGTGCAATAGGACCTC+CGG 0.561409 3.1:+67054136 None:intergenic
TGGTGAGCTTGGATTCATCA+TGG 0.561975 3.1:+67054298 None:intergenic
TGGACCCAAGTTGCTGGCTG+AGG 0.570124 3.1:+67054274 None:intergenic
TTTGACACACGGCCTTCAGA+TGG 0.574107 3.1:+67054254 None:intergenic
AGTAATGGAAGTGTAAGGAG+GGG 0.590782 3.1:+67054361 None:intergenic
CATAGATTGATTCATAGGAT+GGG 0.593968 3.1:+67054040 None:intergenic
GGGTAAAGTTGAAGCAGGGG+TGG 0.597668 3.1:+67054331 None:intergenic
GCCCATACGAAGAAGATCAA+GGG 0.605404 3.1:-67054231 MS.gene70503:CDS
GCCATGGTACAACAATTCAC+CGG 0.607187 3.1:-67054155 MS.gene70503:CDS
TTCATCATGGTTGATTCAGG+GGG 0.612844 3.1:+67054311 None:intergenic
ATTCATCATGGTTGATTCAG+GGG 0.614825 3.1:+67054310 None:intergenic
TGAATTGTTGTACCATGGCA+CGG 0.621886 3.1:+67054159 None:intergenic
TCCGGTGAATTGTTGTACCA+TGG 0.630340 3.1:+67054154 None:intergenic
GCCGACGACTTTACTTAACA+CGG 0.631878 3.1:-67054195 MS.gene70503:CDS
TTCATAGGATGGGAAGCCCG+TGG 0.633148 3.1:+67054050 None:intergenic
AACGTTAATACACAAGTAGG+AGG 0.633926 3.1:-67053930 MS.gene70503:CDS
TCATAGGATGGGAAGCCCGT+GGG 0.636209 3.1:+67054051 None:intergenic
GGCAACGTTAATACACAAGT+AGG 0.638298 3.1:-67053933 MS.gene70503:CDS
ATTAGGTGGTGATGATGATG+TGG 0.666182 3.1:+67054103 None:intergenic
GCTAGGGTTGAGAAATTAGG+TGG 0.673876 3.1:+67054089 None:intergenic
GTTAGTGATCATATTCATGG+AGG 0.678249 3.1:-67053828 MS.gene70503:CDS
TCCGTGTTAAGTAAAGTCGT+CGG 0.679295 3.1:+67054194 None:intergenic
ATGGTACAACAATTCACCGG+AGG 0.714463 3.1:-67054152 MS.gene70503:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTATCATTGTTTGA+AGG + chr3.1:67054352-67054371 None:intergenic 15.0%
!! TAGAATTGAAAAAACTCATT+TGG + chr3.1:67053809-67053828 None:intergenic 20.0%
! AATTGAAAAAACTCATTTGG+TGG + chr3.1:67053806-67053825 None:intergenic 25.0%
! ATTGAAAAAACTCATTTGGT+GGG + chr3.1:67053805-67053824 None:intergenic 25.0%
CAACACTACAATATGTATAG+TGG - chr3.1:67054259-67054278 MS.gene70503:CDS 30.0%
GTTGTTAGTGATCATATTCA+TGG - chr3.1:67054382-67054401 MS.gene70503:CDS 30.0%
TTATCATTGTTTGAAGGTCT+TGG + chr3.1:67054346-67054365 None:intergenic 30.0%
! CATAGATTGATTCATAGGAT+GGG + chr3.1:67054176-67054195 None:intergenic 30.0%
! GACATCATAGATTGATTCAT+AGG + chr3.1:67054181-67054200 None:intergenic 30.0%
! TCATAGATTGATTCATAGGA+TGG + chr3.1:67054177-67054196 None:intergenic 30.0%
! TTAGTAGTAATGGAAGTGTA+AGG + chr3.1:67053860-67053879 None:intergenic 30.0%
! TTGAAAAAACTCATTTGGTG+GGG + chr3.1:67053804-67053823 None:intergenic 30.0%
AACGTTAATACACAAGTAGG+AGG - chr3.1:67054283-67054302 MS.gene70503:CDS 35.0%
GTTAGTGATCATATTCATGG+AGG - chr3.1:67054385-67054404 MS.gene70503:CDS 35.0%
! ATTCATCATGGTTGATTCAG+GGG + chr3.1:67053906-67053925 None:intergenic 35.0%
! GATTCATCATGGTTGATTCA+GGG + chr3.1:67053907-67053926 None:intergenic 35.0%
! TAGTAATGGAAGTGTAAGGA+GGG + chr3.1:67053856-67053875 None:intergenic 35.0%
! TGAAAAAACTCATTTGGTGG+GGG + chr3.1:67053803-67053822 None:intergenic 35.0%
GATCATATTCATGGAGGAGA+AGG - chr3.1:67054391-67054410 MS.gene70503:CDS 40.0%
GGCAACGTTAATACACAAGT+AGG - chr3.1:67054280-67054299 MS.gene70503:CDS 40.0%
TCCGTGTTAAGTAAAGTCGT+CGG + chr3.1:67054022-67054041 None:intergenic 40.0%
TGAATTGTTGTACCATGGCA+CGG + chr3.1:67054057-67054076 None:intergenic 40.0%
TGTAAGGAGGGGTTTGATTA+GGG + chr3.1:67053844-67053863 None:intergenic 40.0%
! AGTAATGGAAGTGTAAGGAG+GGG + chr3.1:67053855-67053874 None:intergenic 40.0%
! ATTAGGTGGTGATGATGATG+TGG + chr3.1:67054113-67054132 None:intergenic 40.0%
! GGATTCATCATGGTTGATTC+AGG + chr3.1:67053908-67053927 None:intergenic 40.0%
! GTAGTAATGGAAGTGTAAGG+AGG + chr3.1:67053857-67053876 None:intergenic 40.0%
! GTTGTTGTTGAAGCTGTTGT+TGG + chr3.1:67054216-67054235 None:intergenic 40.0%
! TGAAGCTGTTGTTGGTAGAA+AGG + chr3.1:67054208-67054227 None:intergenic 40.0%
! TTCATCATGGTTGATTCAGG+GGG + chr3.1:67053905-67053924 None:intergenic 40.0%
AGCCCATACGAAGAAGATCA+AGG - chr3.1:67053981-67054000 MS.gene70503:CDS 45.0%
AGCCCTTGATCTTCTTCGTA+TGG + chr3.1:67053987-67054006 None:intergenic 45.0%
ATGGTACAACAATTCACCGG+AGG - chr3.1:67054061-67054080 MS.gene70503:CDS 45.0%
CACTACTAATTTCCGTGCCA+TGG - chr3.1:67054042-67054061 MS.gene70503:CDS 45.0%
CATATTCATGGAGGAGAAGG+TGG - chr3.1:67054394-67054413 MS.gene70503:CDS 45.0%
CCTAATTTCTCAACCCTAGC+AGG - chr3.1:67054127-67054146 MS.gene70503:CDS 45.0%
CCTGCTAGGGTTGAGAAATT+AGG + chr3.1:67054130-67054149 None:intergenic 45.0%
GCCATGGTACAACAATTCAC+CGG - chr3.1:67054058-67054077 MS.gene70503:CDS 45.0%
GCCCATACGAAGAAGATCAA+GGG - chr3.1:67053982-67054001 MS.gene70503:CDS 45.0%
GCCCTTGATCTTCTTCGTAT+GGG + chr3.1:67053986-67054005 None:intergenic 45.0%
GCCGACGACTTTACTTAACA+CGG - chr3.1:67054018-67054037 MS.gene70503:CDS 45.0%
GCTAGGGTTGAGAAATTAGG+TGG + chr3.1:67054127-67054146 None:intergenic 45.0%
GTGTAAGGAGGGGTTTGATT+AGG + chr3.1:67053845-67053864 None:intergenic 45.0%
TCCGGTGAATTGTTGTACCA+TGG + chr3.1:67054062-67054081 None:intergenic 45.0%
TGGTGAGCTTGGATTCATCA+TGG + chr3.1:67053918-67053937 None:intergenic 45.0%
! AAAAGGTGCAATAGGACCTC+CGG + chr3.1:67054080-67054099 None:intergenic 45.0%
! AGGGGTGGTGTTAGTAGTAA+TGG + chr3.1:67053870-67053889 None:intergenic 45.0%
! TTCGTATGGGCTTTGACACA+CGG + chr3.1:67053973-67053992 None:intergenic 45.0%
AGCAACTTGGGTCCATCTGA+AGG - chr3.1:67053947-67053966 MS.gene70503:CDS 50.0%
AGGGGGTAAAGTTGAAGCAG+GGG + chr3.1:67053888-67053907 None:intergenic 50.0%
CAGGGGGTAAAGTTGAAGCA+GGG + chr3.1:67053889-67053908 None:intergenic 50.0%
GAAGAAGATCAAGGGCTTCG+CGG - chr3.1:67053990-67054009 MS.gene70503:CDS 50.0%
TCAGGGGGTAAAGTTGAAGC+AGG + chr3.1:67053890-67053909 None:intergenic 50.0%
TTCAGATGGACCCAAGTTGC+TGG + chr3.1:67053948-67053967 None:intergenic 50.0%
TTTGACACACGGCCTTCAGA+TGG + chr3.1:67053962-67053981 None:intergenic 50.0%
! GCTGCTGCAAAAGGTGCAAT+AGG + chr3.1:67054088-67054107 None:intergenic 50.0%
ACCAACCTCAGCCAGCAACT+TGG - chr3.1:67053934-67053953 MS.gene70503:CDS 55.0%
CCAACCTCAGCCAGCAACTT+GGG - chr3.1:67053935-67053954 MS.gene70503:CDS 55.0%
GATGTGGCTGCTGCTGCAAA+AGG + chr3.1:67054097-67054116 None:intergenic 55.0%
GGGTAAAGTTGAAGCAGGGG+TGG + chr3.1:67053885-67053904 None:intergenic 55.0%
TCATAGGATGGGAAGCCCGT+GGG + chr3.1:67054165-67054184 None:intergenic 55.0%
TCTCAACCCTAGCAGGCCTA+GGG - chr3.1:67054134-67054153 MS.gene70503:CDS 55.0%
TTCATAGGATGGGAAGCCCG+TGG + chr3.1:67054166-67054185 None:intergenic 55.0%
TTCTCAACCCTAGCAGGCCT+AGG - chr3.1:67054133-67054152 MS.gene70503:CDS 55.0%
! CCCAAGTTGCTGGCTGAGGT+TGG + chr3.1:67053938-67053957 None:intergenic 60.0%
! TGGACCCAAGTTGCTGGCTG+AGG + chr3.1:67053942-67053961 None:intergenic 60.0%
!! CTGGCTGAGGTTGGTGAGCT+TGG + chr3.1:67053929-67053948 None:intergenic 60.0%
ACCCTAGCAGGCCTAGGGCT+TGG - chr3.1:67054139-67054158 MS.gene70503:CDS 65.0%
GCCAAGCCCTAGGCCTGCTA+GGG + chr3.1:67054143-67054162 None:intergenic 65.0%
AAGCCCGTGGGCCAAGCCCT+AGG + chr3.1:67054153-67054172 None:intergenic 70.0%
AGGCCTAGGGCTTGGCCCAC+GGG - chr3.1:67054147-67054166 MS.gene70503:CDS 70.0%
CAGGCCTAGGGCTTGGCCCA+CGG - chr3.1:67054146-67054165 MS.gene70503:CDS 70.0%
GGCCAAGCCCTAGGCCTGCT+AGG + chr3.1:67054144-67054163 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 67053791 67054444 67053791 ID=MS.gene70503
chr3.1 mRNA 67053791 67054444 67053791 ID=MS.gene70503.t1;Parent=MS.gene70503
chr3.1 exon 67053791 67054444 67053791 ID=MS.gene70503.t1.exon1;Parent=MS.gene70503.t1
chr3.1 CDS 67053791 67054444 67053791 ID=cds.MS.gene70503.t1;Parent=MS.gene70503.t1
Gene Sequence

>MS.gene70503

ATGTCTGATCCCCCACCAAATGAGTTTTTTCAATTCTATCATCAAAATTTCCCTAATCAAACCCCTCCTTACACTTCCATTACTACTAACACCACCCCTGCTTCAACTTTACCCCCTGAATCAACCATGATGAATCCAAGCTCACCAACCTCAGCCAGCAACTTGGGTCCATCTGAAGGCCGTGTGTCAAAGCCCATACGAAGAAGATCAAGGGCTTCGCGGCGAACGCCGACGACTTTACTTAACACGGACACTACTAATTTCCGTGCCATGGTACAACAATTCACCGGAGGTCCTATTGCACCTTTTGCAGCAGCAGCCACATCATCATCACCACCTAATTTCTCAACCCTAGCAGGCCTAGGGCTTGGCCCACGGGCTTCCCATCCTATGAATCAATCTATGATGTCTCATCCTTTCTACCAACAACAGCTTCAACAACAACAGCAGCAACAACAACAATATCAACAACACTACAATATGTATAGTGGCAACGTTAATACACAAGTAGGAGGAGATGATAACATGTTTTTTCAGAGAATAATGAGCAATCCAAGACCTTCAAACAATGATAATAATAATATTAATAATGTTGTTAGTGATCATATTCATGGAGGAGAAGGTGGTTTCTTTCCAAGAACTTCTTCTTCTTGA

Protein sequence

>MS.gene70503.t1

MSDPPPNEFFQFYHQNFPNQTPPYTSITTNTTPASTLPPESTMMNPSSPTSASNLGPSEGRVSKPIRRRSRASRRTPTTLLNTDTTNFRAMVQQFTGGPIAPFAAAATSSSPPNFSTLAGLGLGPRASHPMNQSMMSHPFYQQQLQQQQQQQQQYQQHYNMYSGNVNTQVGGDDNMFFQRIMSNPRPSNNDNNNINNVVSDHIHGGEGGFFPRTSSS