Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70651.t1 | XP_003601228.2 | 92.9 | 495 | 34 | 1 | 1 | 494 | 1 | 495 | 2.00E-276 | 961.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70651.t1 | Q08BY5 | 40.0 | 335 | 172 | 6 | 20 | 351 | 34 | 342 | 1.2e-67 | 258.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70651.t1 | G7J4W1 | 92.9 | 495 | 34 | 1 | 1 | 494 | 1 | 495 | 1.5e-276 | 961.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene70651.t1 | TR | Jumonji |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049378 | MS.gene70651 | 0.813928 | 2.00E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene70651 | 0.850371 | 1.77E-60 | -1.69E-46 |
| MS.gene050227 | MS.gene70651 | 0.846956 | 1.57E-59 | -1.69E-46 |
| MS.gene050228 | MS.gene70651 | 0.857312 | 1.78E-62 | -1.69E-46 |
| MS.gene050442 | MS.gene70651 | 0.816879 | 4.41E-52 | -1.69E-46 |
| MS.gene050719 | MS.gene70651 | 0.817545 | 3.12E-52 | -1.69E-46 |
| MS.gene050721 | MS.gene70651 | 0.819394 | 1.19E-52 | -1.69E-46 |
| MS.gene050724 | MS.gene70651 | 0.804831 | 1.79E-49 | -1.69E-46 |
| MS.gene050780 | MS.gene70651 | 0.815686 | 8.14E-52 | -1.69E-46 |
| MS.gene050844 | MS.gene70651 | 0.801839 | 7.45E-49 | -1.69E-46 |
| MS.gene051935 | MS.gene70651 | 0.812186 | 4.81E-51 | -1.69E-46 |
| MS.gene051983 | MS.gene70651 | 0.800224 | 1.59E-48 | -1.69E-46 |
| MS.gene052176 | MS.gene70651 | 0.802183 | 6.33E-49 | -1.69E-46 |
| MS.gene054467 | MS.gene70651 | 0.800429 | 1.45E-48 | -1.69E-46 |
| MS.gene054468 | MS.gene70651 | 0.806318 | 8.72E-50 | -1.69E-46 |
| MS.gene054942 | MS.gene70651 | 0.803914 | 2.78E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene70651 | 0.807437 | 5.06E-50 | -1.69E-46 |
| MS.gene055363 | MS.gene70651 | 0.806226 | 9.12E-50 | -1.69E-46 |
| MS.gene056173 | MS.gene70651 | 0.801489 | 8.79E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene70651 | 0.800222 | 1.59E-48 | -1.69E-46 |
| MS.gene057026 | MS.gene70651 | 0.809043 | 2.30E-50 | -1.69E-46 |
| MS.gene057469 | MS.gene70651 | 0.804247 | 2.37E-49 | -1.69E-46 |
| MS.gene057980 | MS.gene70651 | 0.809476 | 1.86E-50 | -1.69E-46 |
| MS.gene058636 | MS.gene70651 | 0.801872 | 7.34E-49 | -1.69E-46 |
| MS.gene058829 | MS.gene70651 | 0.804383 | 2.22E-49 | -1.69E-46 |
| MS.gene059128 | MS.gene70651 | 0.810908 | 9.12E-51 | -1.69E-46 |
| MS.gene059220 | MS.gene70651 | 0.807504 | 4.90E-50 | -1.69E-46 |
| MS.gene059789 | MS.gene70651 | 0.800973 | 1.12E-48 | -1.69E-46 |
| MS.gene060572 | MS.gene70651 | 0.810659 | 1.03E-50 | -1.69E-46 |
| MS.gene060683 | MS.gene70651 | 0.816036 | 6.80E-52 | -1.69E-46 |
| MS.gene060685 | MS.gene70651 | 0.808807 | 2.58E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70651.t1 | MTR_3g077420 | 93.542 | 480 | 31 | 0 | 15 | 494 | 16 | 495 | 0.0 | 939 |
| MS.gene70651.t1 | MTR_3g077420 | 94.737 | 323 | 17 | 0 | 15 | 337 | 16 | 338 | 0.0 | 653 |
| MS.gene70651.t1 | MTR_3g077420 | 93.728 | 287 | 18 | 0 | 15 | 301 | 16 | 302 | 0.0 | 573 |
| MS.gene70651.t1 | MTR_7g106990 | 27.723 | 303 | 140 | 14 | 7 | 290 | 197 | 439 | 5.22e-18 | 86.7 |
| MS.gene70651.t1 | MTR_3g099370 | 25.000 | 296 | 165 | 13 | 12 | 299 | 132 | 378 | 4.84e-15 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70651.t1 | AT5G63080 | 55.839 | 471 | 184 | 5 | 8 | 476 | 3 | 451 | 0.0 | 533 |
| MS.gene70651.t1 | AT5G06550 | 25.000 | 324 | 157 | 13 | 14 | 319 | 194 | 449 | 2.98e-14 | 75.5 |
| MS.gene70651.t1 | AT1G78280 | 23.411 | 299 | 166 | 10 | 12 | 299 | 125 | 371 | 5.04e-13 | 72.4 |
Find 89 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTAACTTATGCAGACTATT+TGG | 0.218231 | 3.1:+63641472 | MS.gene70651:intron |
| GTATCATCAAGTTCATAATT+TGG | 0.246758 | 3.1:+63641545 | MS.gene70651:CDS |
| CACTTGTTCTGGCAGGATCT+TGG | 0.254470 | 3.1:+63640860 | MS.gene70651:intron |
| GTGCTGCTTTGCTATATATA+TGG | 0.271036 | 3.1:+63642410 | MS.gene70651:CDS |
| CTCAAAATCAGAGGACAAAT+TGG | 0.271585 | 3.1:-63642651 | None:intergenic |
| TCAATGCCTATAACCTTTCT+TGG | 0.293305 | 3.1:+63641771 | MS.gene70651:CDS |
| ACTGATTGGGTTACACCTAA+TGG | 0.293546 | 3.1:+63634055 | MS.gene70651:CDS |
| AGTGGTGTTAACTGGATTAA+TGG | 0.294120 | 3.1:+63634012 | MS.gene70651:CDS |
| GTTATGTATGCTACATATTC+TGG | 0.294875 | 3.1:-63640593 | None:intergenic |
| CATCAACAACATCAAAATAA+AGG | 0.300767 | 3.1:+63633913 | MS.gene70651:CDS |
| TTTGTTTATATGGGAGTTAA+AGG | 0.310574 | 3.1:+63640728 | MS.gene70651:CDS |
| AACAAACCAGTGGTGTTAAC+TGG | 0.321042 | 3.1:+63634004 | MS.gene70651:CDS |
| TGAATTTGTTGATAGATATA+TGG | 0.323277 | 3.1:+63633976 | MS.gene70651:CDS |
| CTTTAAATATTGCGTCTATT+AGG | 0.325382 | 3.1:+63642483 | MS.gene70651:CDS |
| ATAAATTTGATGAGGTCTTC+AGG | 0.341372 | 3.1:-63642743 | None:intergenic |
| GATGTCTTCAATGTATTCCT+TGG | 0.357631 | 3.1:-63641931 | None:intergenic |
| GTTCAGTGCAGTAATGAAAA+TGG | 0.363737 | 3.1:+63634513 | MS.gene70651:CDS |
| CTGGATTAATGGATCATCAT+TGG | 0.366707 | 3.1:+63634023 | MS.gene70651:CDS |
| GAGTGCTTCATTGGTTGATT+TGG | 0.369774 | 3.1:+63642673 | MS.gene70651:CDS |
| TACCACCCAAGAAAGGTTAT+AGG | 0.377980 | 3.1:-63641777 | None:intergenic |
| ATTGGAGAGCTTCCACTGAT+TGG | 0.381303 | 3.1:+63634041 | MS.gene70651:CDS |
| CAATGCCTATAACCTTTCTT+GGG | 0.382210 | 3.1:+63641772 | MS.gene70651:CDS |
| TGACAGTGACCAGCAAAATA+AGG | 0.385417 | 3.1:+63640682 | MS.gene70651:CDS |
| AGACAATACCGTCGCAGAGC+TGG | 0.401710 | 3.1:+63642766 | MS.gene70651:CDS |
| TTGGAGAGCTTCCACTGATT+GGG | 0.421960 | 3.1:+63634042 | MS.gene70651:CDS |
| ATCAACAACATCAAAATAAA+GGG | 0.427273 | 3.1:+63633914 | MS.gene70651:CDS |
| AAACACGTGCATGCTCTAGA+AGG | 0.428150 | 3.1:+63642524 | MS.gene70651:CDS |
| CTGCAGAACCCTCAACTTCA+AGG | 0.428740 | 3.1:-63634491 | None:intergenic |
| TTTCATTTCATACTTTGCCT+TGG | 0.431384 | 3.1:+63642379 | MS.gene70651:CDS |
| CTGTACCGTATTTGAAGGAT+TGG | 0.436080 | 3.1:+63634559 | MS.gene70651:CDS |
| GAGAAATTGAGAAGGTGAAC+GGG | 0.436671 | 3.1:+63633936 | MS.gene70651:CDS |
| CAATATCTATAAACCACAAT+TGG | 0.441545 | 3.1:+63641747 | MS.gene70651:CDS |
| CAGCAAGGTTGCGCTGGCAG+AGG | 0.448536 | 3.1:-63641980 | None:intergenic |
| TTGGTTGATTTGGTGGGACT+CGG | 0.453555 | 3.1:+63642683 | MS.gene70651:CDS |
| CATGCCTGTCAAACACGAAA+TGG | 0.455876 | 3.1:-63640975 | None:intergenic |
| CTCAATTTACCACCCAAGAA+AGG | 0.458313 | 3.1:-63641784 | None:intergenic |
| ATACTTTGCCTTGGCCAACT+TGG | 0.465254 | 3.1:+63642388 | MS.gene70651:CDS |
| TGTGTCTGTACCGTATTTGA+AGG | 0.465358 | 3.1:+63634554 | MS.gene70651:CDS |
| AGTGCCATTTCGTGTTTGAC+AGG | 0.469316 | 3.1:+63640971 | MS.gene70651:CDS |
| TGCTTCATTGGTTGATTTGG+TGG | 0.472657 | 3.1:+63642676 | MS.gene70651:CDS |
| TGAACGTTGCCTTGAAGTTG+AGG | 0.484615 | 3.1:+63634482 | MS.gene70651:CDS |
| GACAATACCGTCGCAGAGCT+GGG | 0.485971 | 3.1:+63642767 | MS.gene70651:CDS |
| TTCTAAATTCTGCATGCAGG+TGG | 0.497798 | 3.1:+63642598 | MS.gene70651:CDS |
| GCTTCATTGGTTGATTTGGT+GGG | 0.500949 | 3.1:+63642677 | MS.gene70651:CDS |
| AATGAAAATGGTTCAAGTAA+TGG | 0.504999 | 3.1:+63634525 | MS.gene70651:CDS |
| CGCAACCTTGCTGCCAACAC+AGG | 0.505053 | 3.1:+63641990 | MS.gene70651:CDS |
| AAATGCCAATCCTTCAAATA+CGG | 0.505177 | 3.1:-63634564 | None:intergenic |
| TGTTGGCAGCAAGGTTGCGC+TGG | 0.509302 | 3.1:-63641986 | None:intergenic |
| TGTCAAACACGAAATGGCAC+TGG | 0.510078 | 3.1:-63640969 | None:intergenic |
| GAACGTTGCCTTGAAGTTGA+GGG | 0.513815 | 3.1:+63634483 | MS.gene70651:CDS |
| TTAGAATGCACACAAGAAGC+AGG | 0.515116 | 3.1:+63641495 | MS.gene70651:CDS |
| ATACAGATGACTCCATGTTA+AGG | 0.518181 | 3.1:-63642793 | None:intergenic |
| TATAGGCATTGAACCAATTG+TGG | 0.520435 | 3.1:-63641760 | None:intergenic |
| TCATATTTGTTCCCAGTGGA+TGG | 0.526700 | 3.1:+63641523 | MS.gene70651:CDS |
| ATTAATCCAGTTAACACCAC+TGG | 0.527498 | 3.1:-63634010 | None:intergenic |
| AACTTGATGATACCATCCAC+TGG | 0.531578 | 3.1:-63641535 | None:intergenic |
| GGAATACATTGAAGACATCA+AGG | 0.541622 | 3.1:+63641935 | MS.gene70651:CDS |
| AAATATTGCGTCTATTAGGA+AGG | 0.550115 | 3.1:+63642487 | MS.gene70651:CDS |
| AAGCAGCACCAAGTTGGCCA+AGG | 0.555336 | 3.1:-63642396 | None:intergenic |
| GTCAAACACGAAATGGCACT+GGG | 0.569616 | 3.1:-63640968 | None:intergenic |
| TACTCTTGACAAACTTTCAG+CGG | 0.571687 | 3.1:-63642447 | None:intergenic |
| TGCCTATAACCTTTCTTGGG+TGG | 0.577403 | 3.1:+63641775 | MS.gene70651:CDS |
| ATGTCTGTGGAAAGAAGAGA+TGG | 0.577930 | 3.1:+63640929 | MS.gene70651:CDS |
| GGAGAAATTGAGAAGGTGAA+CGG | 0.580825 | 3.1:+63633935 | MS.gene70651:CDS |
| TCTAAATTCTGCATGCAGGT+GGG | 0.581693 | 3.1:+63642599 | MS.gene70651:CDS |
| CTGCACCTGTGTTGGCAGCA+AGG | 0.594405 | 3.1:-63641995 | None:intergenic |
| AACACGAAATGGCACTGGGA+AGG | 0.599724 | 3.1:-63640964 | None:intergenic |
| GAGCGACTACAATGAGGCCA+AGG | 0.603057 | 3.1:+63641914 | MS.gene70651:CDS |
| TAGAATGCACACAAGAAGCA+GGG | 0.604713 | 3.1:+63641496 | MS.gene70651:CDS |
| AATTGAGAAGGTGAACGGGA+AGG | 0.605761 | 3.1:+63633940 | MS.gene70651:CDS |
| AAGGTCGCCCAGCTCTGCGA+CGG | 0.606124 | 3.1:-63642774 | None:intergenic |
| ACACAGTACTGCACCTGTGT+TGG | 0.611154 | 3.1:-63642003 | None:intergenic |
| AACACGTGCATGCTCTAGAA+GGG | 0.614914 | 3.1:+63642525 | MS.gene70651:CDS |
| AGAGCTGGGCGACCTTAACA+TGG | 0.618534 | 3.1:+63642781 | MS.gene70651:CDS |
| ATAGCAAAGCAGCACCAAGT+TGG | 0.619753 | 3.1:-63642402 | None:intergenic |
| ATTAGGTGTAACCCAATCAG+TGG | 0.622628 | 3.1:-63634053 | None:intergenic |
| AGCTGGTCAGCAAATGTCTG+TGG | 0.623034 | 3.1:+63640916 | MS.gene70651:CDS |
| TATTGTCTATAAATTTGATG+AGG | 0.630189 | 3.1:-63642751 | None:intergenic |
| ATGCAGAATTTAGAAAACCT+AGG | 0.631755 | 3.1:-63642590 | None:intergenic |
| CTGAAAACATCAGCATGTAG+AGG | 0.641625 | 3.1:-63640886 | None:intergenic |
| TGAAGCACTCTCAAAATCAG+AGG | 0.642152 | 3.1:-63642660 | None:intergenic |
| GTATCTGACTCAAAATACCC+TGG | 0.643979 | 3.1:+63641364 | MS.gene70651:CDS |
| AATAAAGGGAGAAATTGAGA+AGG | 0.646492 | 3.1:+63633928 | MS.gene70651:CDS |
| ACTTGATGATACCATCCACT+GGG | 0.651707 | 3.1:-63641534 | None:intergenic |
| AGAATGCACACAAGAAGCAG+GGG | 0.665619 | 3.1:+63641497 | MS.gene70651:CDS |
| GAAATCATATTTGTTCCCAG+TGG | 0.671903 | 3.1:+63641519 | MS.gene70651:CDS |
| CAATCATCACAAAAGTACGT+TGG | 0.708793 | 3.1:-63640617 | None:intergenic |
| TATGGACAAGAACAAACCAG+TGG | 0.727874 | 3.1:+63633994 | MS.gene70651:CDS |
| AGGGACTCATGATTGCATCG+TGG | 0.769193 | 3.1:+63642544 | MS.gene70651:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 63633902 | 63642829 | 63633902 | ID=MS.gene70651 |
| chr3.1 | mRNA | 63633902 | 63642829 | 63633902 | ID=MS.gene70651.t1;Parent=MS.gene70651 |
| chr3.1 | exon | 63633902 | 63634129 | 63633902 | ID=MS.gene70651.t1.exon1;Parent=MS.gene70651.t1 |
| chr3.1 | CDS | 63633902 | 63634129 | 63633902 | ID=cds.MS.gene70651.t1;Parent=MS.gene70651.t1 |
| chr3.1 | exon | 63634414 | 63634593 | 63634414 | ID=MS.gene70651.t1.exon2;Parent=MS.gene70651.t1 |
| chr3.1 | CDS | 63634414 | 63634593 | 63634414 | ID=cds.MS.gene70651.t1;Parent=MS.gene70651.t1 |
| chr3.1 | exon | 63640587 | 63640749 | 63640587 | ID=MS.gene70651.t1.exon3;Parent=MS.gene70651.t1 |
| chr3.1 | CDS | 63640587 | 63640749 | 63640587 | ID=cds.MS.gene70651.t1;Parent=MS.gene70651.t1 |
| chr3.1 | exon | 63640875 | 63640992 | 63640875 | ID=MS.gene70651.t1.exon4;Parent=MS.gene70651.t1 |
| chr3.1 | CDS | 63640875 | 63640992 | 63640875 | ID=cds.MS.gene70651.t1;Parent=MS.gene70651.t1 |
| chr3.1 | exon | 63641327 | 63641396 | 63641327 | ID=MS.gene70651.t1.exon5;Parent=MS.gene70651.t1 |
| chr3.1 | CDS | 63641327 | 63641396 | 63641327 | ID=cds.MS.gene70651.t1;Parent=MS.gene70651.t1 |
| chr3.1 | exon | 63641486 | 63641566 | 63641486 | ID=MS.gene70651.t1.exon6;Parent=MS.gene70651.t1 |
| chr3.1 | CDS | 63641486 | 63641566 | 63641486 | ID=cds.MS.gene70651.t1;Parent=MS.gene70651.t1 |
| chr3.1 | exon | 63641740 | 63641796 | 63641740 | ID=MS.gene70651.t1.exon7;Parent=MS.gene70651.t1 |
| chr3.1 | CDS | 63641740 | 63641796 | 63641740 | ID=cds.MS.gene70651.t1;Parent=MS.gene70651.t1 |
| chr3.1 | exon | 63641900 | 63642011 | 63641900 | ID=MS.gene70651.t1.exon8;Parent=MS.gene70651.t1 |
| chr3.1 | CDS | 63641900 | 63642011 | 63641900 | ID=cds.MS.gene70651.t1;Parent=MS.gene70651.t1 |
| chr3.1 | exon | 63642354 | 63642829 | 63642354 | ID=MS.gene70651.t1.exon9;Parent=MS.gene70651.t1 |
| chr3.1 | CDS | 63642354 | 63642829 | 63642354 | ID=cds.MS.gene70651.t1;Parent=MS.gene70651.t1 |
| Gene Sequence |
| Protein sequence |
