Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70825.t1 | XP_013468980.1 | 96.8 | 253 | 6 | 2 | 1 | 251 | 1 | 253 | 1.60E-131 | 478.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70825.t1 | O24407 | 66.0 | 250 | 59 | 6 | 14 | 251 | 1 | 236 | 2.9e-78 | 293.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70825.t1 | A0A072VMG8 | 96.8 | 253 | 6 | 2 | 1 | 251 | 1 | 253 | 1.1e-131 | 478.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene70825.t1 | TR | AUX/IAA |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene70825 | 0.821262 | 4.43E-53 | -1.69E-46 |
| MS.gene049647 | MS.gene70825 | 0.806776 | 6.98E-50 | -1.69E-46 |
| MS.gene050762 | MS.gene70825 | 0.826823 | 2.20E-54 | -1.69E-46 |
| MS.gene050763 | MS.gene70825 | 0.825102 | 5.63E-54 | -1.69E-46 |
| MS.gene050807 | MS.gene70825 | 0.825977 | 3.49E-54 | -1.69E-46 |
| MS.gene050826 | MS.gene70825 | 0.828021 | 1.13E-54 | -1.69E-46 |
| MS.gene051102 | MS.gene70825 | 0.811175 | 7.99E-51 | -1.69E-46 |
| MS.gene052665 | MS.gene70825 | 0.811699 | 6.14E-51 | -1.69E-46 |
| MS.gene052673 | MS.gene70825 | -0.829453 | 5.10E-55 | -1.69E-46 |
| MS.gene054379 | MS.gene70825 | 0.834614 | 2.71E-56 | -1.69E-46 |
| MS.gene054467 | MS.gene70825 | 0.805531 | 1.28E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene70825 | 0.823195 | 1.58E-53 | -1.69E-46 |
| MS.gene055469 | MS.gene70825 | 0.827655 | 1.39E-54 | -1.69E-46 |
| MS.gene055926 | MS.gene70825 | 0.831173 | 1.94E-55 | -1.69E-46 |
| MS.gene05604 | MS.gene70825 | 0.833348 | 5.61E-56 | -1.69E-46 |
| MS.gene056308 | MS.gene70825 | 0.818102 | 2.34E-52 | -1.69E-46 |
| MS.gene058638 | MS.gene70825 | 0.82358 | 1.28E-53 | -1.69E-46 |
| MS.gene058639 | MS.gene70825 | 0.830782 | 2.42E-55 | -1.69E-46 |
| MS.gene058647 | MS.gene70825 | 0.807927 | 3.98E-50 | -1.69E-46 |
| MS.gene059438 | MS.gene70825 | 0.801213 | 1.00E-48 | -1.69E-46 |
| MS.gene059439 | MS.gene70825 | 0.850041 | 2.19E-60 | -1.69E-46 |
| MS.gene059440 | MS.gene70825 | 0.805226 | 1.48E-49 | -1.69E-46 |
| MS.gene059561 | MS.gene70825 | 0.812109 | 5.00E-51 | -1.69E-46 |
| MS.gene059570 | MS.gene70825 | 0.805706 | 1.17E-49 | -1.69E-46 |
| MS.gene059713 | MS.gene70825 | 0.821695 | 3.52E-53 | -1.69E-46 |
| MS.gene06052 | MS.gene70825 | 0.83297 | 6.98E-56 | -1.69E-46 |
| MS.gene060637 | MS.gene70825 | 0.827041 | 1.95E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene062256 | MS.gene70825 | PPI |
| MS.gene004875 | MS.gene70825 | PPI |
| MS.gene85573 | MS.gene70825 | PPI |
| MS.gene70825 | MS.gene07402 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70825.t1 | MTR_1g080860 | 96.838 | 253 | 6 | 2 | 1 | 251 | 1 | 253 | 0.0 | 496 |
| MS.gene70825.t1 | MTR_1g080860 | 96.281 | 242 | 7 | 2 | 1 | 240 | 1 | 242 | 5.95e-162 | 448 |
| MS.gene70825.t1 | MTR_1g093240 | 64.876 | 242 | 67 | 5 | 15 | 251 | 8 | 236 | 9.37e-100 | 290 |
| MS.gene70825.t1 | MTR_1g070520 | 65.306 | 245 | 61 | 7 | 17 | 251 | 15 | 245 | 5.48e-97 | 284 |
| MS.gene70825.t1 | MTR_1g093240 | 63.478 | 230 | 66 | 5 | 15 | 239 | 8 | 224 | 2.70e-90 | 266 |
| MS.gene70825.t1 | MTR_7g096090 | 60.656 | 244 | 85 | 4 | 15 | 251 | 11 | 250 | 3.34e-89 | 264 |
| MS.gene70825.t1 | MTR_1g093240 | 60.476 | 210 | 65 | 5 | 15 | 219 | 8 | 204 | 8.84e-76 | 228 |
| MS.gene70825.t1 | MTR_5g030710 | 47.917 | 288 | 96 | 9 | 15 | 251 | 51 | 335 | 1.68e-75 | 232 |
| MS.gene70825.t1 | MTR_6g488150 | 50.190 | 263 | 80 | 8 | 15 | 250 | 15 | 253 | 3.97e-74 | 226 |
| MS.gene70825.t1 | MTR_8g103030 | 48.029 | 279 | 92 | 7 | 15 | 250 | 24 | 292 | 5.38e-74 | 227 |
| MS.gene70825.t1 | MTR_8g067530 | 47.387 | 287 | 96 | 8 | 15 | 251 | 75 | 356 | 2.69e-73 | 228 |
| MS.gene70825.t1 | MTR_5g067350 | 47.719 | 285 | 97 | 7 | 15 | 250 | 65 | 346 | 1.56e-71 | 223 |
| MS.gene70825.t1 | MTR_2g101500 | 44.805 | 308 | 95 | 9 | 13 | 250 | 23 | 325 | 5.30e-71 | 221 |
| MS.gene70825.t1 | MTR_1g070830 | 47.162 | 229 | 80 | 5 | 15 | 240 | 12 | 202 | 7.27e-55 | 175 |
| MS.gene70825.t1 | MTR_1g040675 | 43.805 | 226 | 84 | 6 | 15 | 240 | 1 | 183 | 6.70e-53 | 170 |
| MS.gene70825.t1 | MTR_1g076270 | 46.222 | 225 | 64 | 8 | 15 | 239 | 11 | 178 | 1.34e-52 | 169 |
| MS.gene70825.t1 | MTR_1g093350 | 46.222 | 225 | 64 | 8 | 15 | 239 | 11 | 178 | 1.34e-52 | 169 |
| MS.gene70825.t1 | MTR_1g040675 | 40.488 | 205 | 79 | 7 | 15 | 219 | 1 | 162 | 2.55e-40 | 137 |
| MS.gene70825.t1 | MTR_1g069495 | 43.716 | 183 | 71 | 4 | 96 | 246 | 81 | 263 | 7.42e-37 | 131 |
| MS.gene70825.t1 | MTR_4g011880 | 41.991 | 231 | 68 | 9 | 15 | 243 | 6 | 172 | 4.55e-36 | 126 |
| MS.gene70825.t1 | MTR_2g102490 | 40.541 | 222 | 72 | 10 | 18 | 234 | 17 | 183 | 1.58e-34 | 123 |
| MS.gene70825.t1 | MTR_2g100780 | 36.220 | 254 | 122 | 10 | 21 | 242 | 18 | 263 | 5.54e-33 | 121 |
| MS.gene70825.t1 | MTR_2g100780 | 36.327 | 245 | 118 | 9 | 21 | 235 | 18 | 254 | 6.62e-33 | 120 |
| MS.gene70825.t1 | MTR_8g014520 | 35.223 | 247 | 126 | 10 | 21 | 235 | 16 | 260 | 1.16e-31 | 117 |
| MS.gene70825.t1 | MTR_8g014520 | 35.223 | 247 | 126 | 10 | 21 | 235 | 16 | 260 | 1.20e-31 | 117 |
| MS.gene70825.t1 | MTR_6g488150 | 41.848 | 184 | 58 | 7 | 15 | 173 | 15 | 174 | 1.03e-30 | 113 |
| MS.gene70825.t1 | MTR_5g067350 | 39.423 | 208 | 75 | 6 | 15 | 173 | 65 | 270 | 3.31e-28 | 108 |
| MS.gene70825.t1 | MTR_4g124300 | 35.461 | 141 | 61 | 4 | 95 | 233 | 69 | 181 | 6.23e-18 | 79.3 |
| MS.gene70825.t1 | MTR_3g106850 | 30.000 | 140 | 79 | 4 | 97 | 235 | 88 | 209 | 7.60e-18 | 79.7 |
| MS.gene70825.t1 | MTR_1g085750 | 39.796 | 98 | 47 | 3 | 135 | 232 | 85 | 170 | 2.51e-15 | 72.0 |
| MS.gene70825.t1 | MTR_7g110790 | 38.095 | 105 | 45 | 3 | 132 | 232 | 67 | 155 | 3.33e-15 | 71.2 |
| MS.gene70825.t1 | MTR_4g115075 | 27.815 | 151 | 86 | 3 | 82 | 232 | 78 | 205 | 4.75e-15 | 72.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70825.t1 | AT3G04730 | 66.000 | 250 | 59 | 6 | 14 | 251 | 1 | 236 | 8.49e-103 | 298 |
| MS.gene70825.t1 | AT4G14550 | 64.706 | 238 | 73 | 4 | 15 | 251 | 1 | 228 | 3.34e-99 | 289 |
| MS.gene70825.t1 | AT3G23050 | 62.195 | 246 | 78 | 6 | 14 | 251 | 5 | 243 | 2.01e-97 | 285 |
| MS.gene70825.t1 | AT4G14550 | 63.436 | 227 | 72 | 4 | 15 | 240 | 1 | 217 | 2.81e-91 | 269 |
| MS.gene70825.t1 | AT1G04250 | 57.741 | 239 | 83 | 6 | 15 | 251 | 7 | 229 | 1.10e-87 | 259 |
| MS.gene70825.t1 | AT4G29080 | 51.799 | 278 | 93 | 9 | 9 | 250 | 32 | 304 | 1.69e-78 | 239 |
| MS.gene70825.t1 | AT4G14550 | 60.976 | 205 | 69 | 4 | 15 | 218 | 1 | 195 | 2.50e-76 | 229 |
| MS.gene70825.t1 | AT3G23050 | 58.216 | 213 | 75 | 5 | 14 | 219 | 5 | 210 | 6.67e-75 | 226 |
| MS.gene70825.t1 | AT5G65670 | 48.264 | 288 | 101 | 9 | 7 | 251 | 54 | 336 | 1.70e-68 | 214 |
| MS.gene70825.t1 | AT2G22670 | 45.357 | 280 | 101 | 6 | 15 | 250 | 47 | 318 | 2.19e-67 | 211 |
| MS.gene70825.t1 | AT2G22670 | 45.357 | 280 | 101 | 6 | 15 | 250 | 47 | 318 | 2.19e-67 | 211 |
| MS.gene70825.t1 | AT2G22670 | 45.357 | 280 | 101 | 6 | 15 | 250 | 64 | 335 | 2.91e-67 | 211 |
| MS.gene70825.t1 | AT5G65670 | 47.931 | 290 | 101 | 10 | 7 | 251 | 54 | 338 | 1.31e-66 | 210 |
| MS.gene70825.t1 | AT2G22670 | 44.643 | 280 | 101 | 7 | 15 | 250 | 47 | 316 | 4.49e-64 | 202 |
| MS.gene70825.t1 | AT2G22670 | 44.643 | 280 | 101 | 7 | 15 | 250 | 47 | 316 | 4.49e-64 | 202 |
| MS.gene70825.t1 | AT1G04240 | 47.137 | 227 | 76 | 7 | 14 | 240 | 4 | 186 | 1.57e-59 | 187 |
| MS.gene70825.t1 | AT1G04240 | 47.137 | 227 | 76 | 7 | 14 | 240 | 104 | 286 | 2.92e-58 | 187 |
| MS.gene70825.t1 | AT5G43700 | 48.899 | 227 | 62 | 9 | 14 | 240 | 10 | 182 | 3.07e-58 | 183 |
| MS.gene70825.t1 | AT4G14550 | 57.500 | 160 | 57 | 4 | 15 | 173 | 1 | 150 | 7.21e-48 | 157 |
| MS.gene70825.t1 | AT3G23050 | 53.293 | 167 | 64 | 5 | 14 | 173 | 5 | 164 | 2.10e-45 | 150 |
| MS.gene70825.t1 | AT3G23030 | 41.453 | 234 | 66 | 5 | 10 | 239 | 4 | 170 | 2.38e-45 | 150 |
| MS.gene70825.t1 | AT3G23030 | 41.453 | 234 | 66 | 5 | 10 | 239 | 91 | 257 | 1.12e-44 | 151 |
| MS.gene70825.t1 | AT4G14560 | 40.991 | 222 | 67 | 6 | 15 | 236 | 7 | 164 | 1.99e-43 | 145 |
| MS.gene70825.t1 | AT3G15540 | 42.739 | 241 | 80 | 10 | 15 | 246 | 6 | 197 | 2.28e-43 | 145 |
| MS.gene70825.t1 | AT2G33310 | 43.684 | 190 | 85 | 6 | 73 | 245 | 53 | 237 | 3.45e-39 | 136 |
| MS.gene70825.t1 | AT1G15580 | 40.724 | 221 | 61 | 7 | 15 | 234 | 8 | 159 | 1.60e-38 | 132 |
| MS.gene70825.t1 | AT2G33310 | 43.158 | 190 | 85 | 6 | 73 | 245 | 53 | 236 | 8.68e-38 | 133 |
| MS.gene70825.t1 | AT2G33310 | 43.158 | 190 | 85 | 6 | 73 | 245 | 53 | 236 | 8.68e-38 | 133 |
| MS.gene70825.t1 | AT1G04550 | 43.085 | 188 | 70 | 6 | 83 | 246 | 56 | 230 | 2.62e-37 | 131 |
| MS.gene70825.t1 | AT1G52830 | 38.938 | 226 | 92 | 8 | 15 | 240 | 6 | 185 | 1.39e-35 | 125 |
| MS.gene70825.t1 | AT3G16500 | 36.290 | 248 | 117 | 9 | 8 | 235 | 26 | 252 | 5.53e-35 | 126 |
| MS.gene70825.t1 | AT3G16500 | 36.290 | 248 | 117 | 9 | 8 | 235 | 26 | 252 | 6.51e-35 | 126 |
| MS.gene70825.t1 | AT4G28640 | 37.179 | 234 | 112 | 7 | 29 | 240 | 22 | 242 | 3.57e-33 | 121 |
| MS.gene70825.t1 | AT4G28640 | 37.069 | 232 | 111 | 7 | 29 | 238 | 22 | 240 | 8.09e-33 | 120 |
| MS.gene70825.t1 | AT4G28640 | 37.069 | 232 | 111 | 7 | 29 | 238 | 22 | 240 | 3.13e-32 | 120 |
| MS.gene70825.t1 | AT1G51950 | 36.905 | 252 | 102 | 10 | 10 | 235 | 30 | 250 | 3.50e-31 | 116 |
| MS.gene70825.t1 | AT5G25890 | 36.842 | 190 | 93 | 5 | 54 | 242 | 7 | 170 | 1.44e-27 | 104 |
| MS.gene70825.t1 | AT1G04100 | 36.694 | 248 | 123 | 8 | 24 | 240 | 16 | 260 | 1.02e-26 | 104 |
| MS.gene70825.t1 | AT3G17600 | 34.848 | 132 | 61 | 3 | 101 | 232 | 48 | 154 | 1.02e-21 | 88.6 |
| MS.gene70825.t1 | AT2G46990 | 39.583 | 96 | 45 | 1 | 135 | 230 | 86 | 168 | 1.12e-16 | 75.9 |
| MS.gene70825.t1 | AT3G62100 | 38.776 | 98 | 47 | 1 | 135 | 232 | 84 | 168 | 1.59e-16 | 75.1 |
| MS.gene70825.t1 | AT4G32280 | 27.684 | 177 | 81 | 4 | 85 | 232 | 82 | 240 | 5.26e-16 | 75.5 |
| MS.gene70825.t1 | AT4G32280 | 35.714 | 98 | 45 | 2 | 135 | 232 | 119 | 198 | 9.29e-16 | 73.9 |
| MS.gene70825.t1 | AT1G80390 | 33.553 | 152 | 55 | 4 | 20 | 171 | 19 | 124 | 3.58e-13 | 65.1 |
| MS.gene70825.t1 | AT2G46990 | 38.824 | 85 | 39 | 1 | 135 | 219 | 86 | 157 | 5.89e-12 | 62.4 |
| MS.gene70825.t1 | AT3G62100 | 37.647 | 85 | 40 | 1 | 135 | 219 | 84 | 155 | 1.84e-11 | 61.2 |
Find 53 sgRNAs with CRISPR-Local
Find 146 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTAACTCTTGTACAATTTA+AGG | 0.136546 | 1.2:-61306609 | None:intergenic |
| GATGAAGCAGAATCATTTAT+AGG | 0.292641 | 1.2:-61304406 | None:intergenic |
| TCATAAAGTCCTTGGTAACT+TGG | 0.357262 | 1.2:-61306693 | None:intergenic |
| TGCTTGTTGGTGATGTTCCT+TGG | 0.360170 | 1.2:+61306791 | MS.gene70825:CDS |
| TTCTTTCTAAAGGATCTAAC+TGG | 0.382249 | 1.2:-61306466 | None:intergenic |
| GAGTTACGACTCGGTCTTCC+AGG | 0.382644 | 1.2:+61304289 | MS.gene70825:CDS |
| ATTTACTATGTTCTTTCTAA+AGG | 0.395708 | 1.2:-61306476 | None:intergenic |
| AGGTCCACCTTACGTAGATA+TGG | 0.411796 | 1.2:-61306589 | None:intergenic |
| ATGAAAGGTTCCGAGGCAAT+TGG | 0.416704 | 1.2:+61307097 | MS.gene70825:CDS |
| CCTTCACAATTGACAATTGT+GGG | 0.417767 | 1.2:+61306668 | MS.gene70825:CDS |
| GCTTGTTGGTGATGTTCCTT+GGG | 0.420684 | 1.2:+61306792 | MS.gene70825:CDS |
| AAGAGAAAATGACTAACGTT+GGG | 0.423757 | 1.2:+61304221 | None:intergenic |
| GCTTTCATTCATAAAGTCCT+TGG | 0.434299 | 1.2:-61306701 | None:intergenic |
| AGCACAAGTGGTGGTGGGAA+TGG | 0.463834 | 1.2:+61306535 | MS.gene70825:CDS |
| CCAGCGCATCTGACAGCTCT+TGG | 0.472063 | 1.2:-61306630 | None:intergenic |
| TGAAAGGTTCCGAGGCAATT+GGG | 0.476415 | 1.2:+61307098 | MS.gene70825:CDS |
| AATTTGTAGGGCACAAGTTG+TGG | 0.477874 | 1.2:+61306435 | MS.gene70825:intron |
| CATAATCAGAACCGTTCAAA+AGG | 0.478352 | 1.2:-61306735 | None:intergenic |
| CCATCTTTGTCTTCATATGT+TGG | 0.478384 | 1.2:-61306760 | None:intergenic |
| CCAAGAGCTGTCAGATGCGC+TGG | 0.479602 | 1.2:+61306630 | MS.gene70825:CDS |
| GATAAGAGCACAAGTGGTGG+TGG | 0.485348 | 1.2:+61306529 | MS.gene70825:CDS |
| TCCTTCACAATTGACAATTG+TGG | 0.492530 | 1.2:+61306667 | MS.gene70825:CDS |
| ATGAAGACAAAGATGGTGAT+TGG | 0.495668 | 1.2:+61306767 | MS.gene70825:CDS |
| GATGGTGATTGGATGCTTGT+TGG | 0.521825 | 1.2:+61306778 | MS.gene70825:CDS |
| GGTTCCGAGGCAATTGGGCT+AGG | 0.522558 | 1.2:+61307103 | MS.gene70825:CDS |
| CATAAAGTCCTTGGTAACTT+GGG | 0.531177 | 1.2:-61306692 | None:intergenic |
| TTTACCTAGCCCAATTGCCT+CGG | 0.542690 | 1.2:-61307107 | None:intergenic |
| TGGAAGACCGAGTCGTAACT+CGG | 0.546250 | 1.2:-61304287 | None:intergenic |
| TGGGAATGGAGCATTTGTTA+AGG | 0.549372 | 1.2:+61306549 | MS.gene70825:CDS |
| ATTTGTAGGGCACAAGTTGT+GGG | 0.551340 | 1.2:+61306436 | MS.gene70825:intron |
| CTTCCAGGTGCATGTGATCA+CGG | 0.554985 | 1.2:+61304304 | MS.gene70825:CDS |
| AAAGAGAAAATGACTAACGT+TGG | 0.560324 | 1.2:+61304220 | None:intergenic |
| ACTCACTTAGCACGAGGAGG+AGG | 0.560950 | 1.2:-61304496 | None:intergenic |
| TTTGTTAAGGTGAGCATGGA+TGG | 0.571730 | 1.2:+61306562 | MS.gene70825:CDS |
| GAAGCAGATAAGAGCACAAG+TGG | 0.581742 | 1.2:+61306523 | MS.gene70825:CDS |
| CCAACATATGAAGACAAAGA+TGG | 0.591098 | 1.2:+61306760 | MS.gene70825:CDS |
| TCTCCGTGATCACATGCACC+TGG | 0.592832 | 1.2:-61304307 | None:intergenic |
| GTAGGGCACAAGTTGTGGGA+TGG | 0.599917 | 1.2:+61306440 | MS.gene70825:intron |
| AGCATTTGTTAAGGTGAGCA+TGG | 0.602441 | 1.2:+61306558 | MS.gene70825:CDS |
| CCCACAATTGTCAATTGTGA+AGG | 0.603780 | 1.2:-61306668 | None:intergenic |
| TGGTGCACCATATCTACGTA+AGG | 0.613344 | 1.2:+61306582 | MS.gene70825:CDS |
| TTTCTAAAGGATCTAACTGG+TGG | 0.617257 | 1.2:-61306463 | None:intergenic |
| GCAGATAAGAGCACAAGTGG+TGG | 0.620410 | 1.2:+61306526 | MS.gene70825:CDS |
| AAGCGTCTTCGCATAATGAA+AGG | 0.624465 | 1.2:+61307082 | MS.gene70825:CDS |
| GCTTCTGTAGCTGAAAACAA+AGG | 0.627006 | 1.2:+61304442 | MS.gene70825:CDS |
| ATAAGAGCACAAGTGGTGGT+GGG | 0.631680 | 1.2:+61306530 | MS.gene70825:CDS |
| GAGGAGACCGAGTTACGACT+CGG | 0.639589 | 1.2:+61304280 | MS.gene70825:CDS |
| AAGAGGGGAACTCACTCCCA+AGG | 0.640728 | 1.2:-61306808 | None:intergenic |
| AGAGAAAATGACTAACGTTG+GGG | 0.657706 | 1.2:+61304222 | None:intergenic |
| TTGTGGGTCCCAAGTTACCA+AGG | 0.660834 | 1.2:+61306684 | MS.gene70825:CDS |
| TCGCATAATGAAAGGTTCCG+AGG | 0.668062 | 1.2:+61307090 | MS.gene70825:CDS |
| TGCACCATATCTACGTAAGG+TGG | 0.669757 | 1.2:+61306585 | MS.gene70825:CDS |
| AAAACTCACTTAGCACGAGG+AGG | 0.704960 | 1.2:-61304499 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGATTAGATTTTTTTTTTA+AGG | + | chr1.2:61304833-61304852 | MS.gene70825:intron | 10.0% |
| !!! | AATAATAGTATTATTTTAGT+TGG | + | chr1.2:61305902-61305921 | MS.gene70825:intron | 10.0% |
| !!! | AATTTTATTCAAAAAATTTG+AGG | - | chr1.2:61305578-61305597 | None:intergenic | 10.0% |
| !!! | AGATTAGATTTTTTTTTTAA+GGG | + | chr1.2:61304834-61304853 | MS.gene70825:intron | 10.0% |
| !!! | ATTTTGAATAAGTTTTAATT+AGG | + | chr1.2:61304907-61304926 | MS.gene70825:intron | 10.0% |
| !!! | CTTATAAAAATGTTTTAATT+AGG | + | chr1.2:61304933-61304952 | MS.gene70825:intron | 10.0% |
| !! | AATTAACAATATTATATGAG+TGG | - | chr1.2:61306011-61306030 | None:intergenic | 15.0% |
| !! | ATATTATTGTTTAACAAAAC+AGG | - | chr1.2:61305330-61305349 | None:intergenic | 15.0% |
| !! | ATTAAAATTCATGTCAAAAT+AGG | - | chr1.2:61305604-61305623 | None:intergenic | 15.0% |
| !! | ATTTGTGTTTAATTAGAAAT+TGG | + | chr1.2:61306170-61306189 | MS.gene70825:intron | 15.0% |
| !! | TCAAATGTATTTAACAAATT+AGG | + | chr1.2:61307156-61307175 | MS.gene70825:intron | 15.0% |
| !! | TGAATAATTAAGTTATATCT+AGG | + | chr1.2:61304639-61304658 | MS.gene70825:intron | 15.0% |
| !!! | AAAAAAAATAAAGTATTGGA+TGG | + | chr1.2:61305851-61305870 | MS.gene70825:intron | 15.0% |
| !!! | AAGTTTAATGTTAGTTTTTA+TGG | - | chr1.2:61305387-61305406 | None:intergenic | 15.0% |
| !!! | ACGATGTAATAAATTTTTAT+TGG | - | chr1.2:61306945-61306964 | None:intergenic | 15.0% |
| !!! | AGTTTTTAAAAGAATAAACA+TGG | + | chr1.2:61305731-61305750 | MS.gene70825:intron | 15.0% |
| !!! | TGTAATAAAATTGATTTTGA+AGG | - | chr1.2:61305672-61305691 | None:intergenic | 15.0% |
| !! | AAATTAAACAATGTAATGTG+AGG | - | chr1.2:61306841-61306860 | None:intergenic | 20.0% |
| !! | AAGGATAAATAAAGTTGAAA+AGG | + | chr1.2:61306042-61306061 | MS.gene70825:intron | 20.0% |
| !! | ATTTACTATGTTCTTTCTAA+AGG | - | chr1.2:61306479-61306498 | None:intergenic | 20.0% |
| !! | ATTTGTTTGTGAAAAACTAT+TGG | + | chr1.2:61305107-61305126 | MS.gene70825:intron | 20.0% |
| !! | CAATGAATATTGAATTTGTA+GGG | + | chr1.2:61306423-61306442 | MS.gene70825:intron | 20.0% |
| !! | CATAATTTACTGTAGTATAA+AGG | + | chr1.2:61306139-61306158 | MS.gene70825:intron | 20.0% |
| !! | GTAAATTATGCATGAATTAT+TGG | - | chr1.2:61306129-61306148 | None:intergenic | 20.0% |
| !! | TCAATGAATATTGAATTTGT+AGG | + | chr1.2:61306422-61306441 | MS.gene70825:intron | 20.0% |
| !! | TCATAGAGTATAGATAATTA+AGG | + | chr1.2:61304766-61304785 | MS.gene70825:intron | 20.0% |
| !!! | AATGTTTTAATTAGGTTAGA+AGG | + | chr1.2:61304941-61304960 | MS.gene70825:intron | 20.0% |
| !!! | ACTTTATTTATCCTTTTGTT+TGG | - | chr1.2:61306037-61306056 | None:intergenic | 20.0% |
| !!! | ATCTAATCTTTTCAATTCAA+TGG | - | chr1.2:61304823-61304842 | None:intergenic | 20.0% |
| !!! | CTTTATTTATCCTTTTGTTT+GGG | - | chr1.2:61306036-61306055 | None:intergenic | 20.0% |
| !!! | TTTATTTATCCTTTTGTTTG+GGG | - | chr1.2:61306035-61306054 | None:intergenic | 20.0% |
| !!! | TTTTTTGAACATGACAATTT+TGG | + | chr1.2:61305051-61305070 | MS.gene70825:intron | 20.0% |
| ! | AGACTATATAACAATGTGAT+AGG | - | chr1.2:61306102-61306121 | None:intergenic | 25.0% |
| ! | ATAAGATAAGATGTACCATA+TGG | - | chr1.2:61307189-61307208 | None:intergenic | 25.0% |
| ! | GTCTGTTTAATAAGATTAGT+TGG | + | chr1.2:61306374-61306393 | MS.gene70825:intron | 25.0% |
| ! | TTATCTATACTCTATGAACA+AGG | - | chr1.2:61304763-61304782 | None:intergenic | 25.0% |
| !! | AAATTAGGTTTAGAACCATA+TGG | + | chr1.2:61307171-61307190 | MS.gene70825:intron | 25.0% |
| !! | AAATTGATTGACCTTTTGAA+CGG | + | chr1.2:61306724-61306743 | MS.gene70825:CDS | 25.0% |
| !! | AATTTTTCTTGCACATAATC+TGG | - | chr1.2:61305817-61305836 | None:intergenic | 25.0% |
| !! | ATAATCTGGATGACATTTTT+AGG | - | chr1.2:61305803-61305822 | None:intergenic | 25.0% |
| !! | GTTTCTTGTTCCATAATTTT+TGG | + | chr1.2:61304999-61305018 | MS.gene70825:intron | 25.0% |
| !!! | AAAAATAAAGTATTGGATGG+TGG | + | chr1.2:61305854-61305873 | MS.gene70825:intron | 25.0% |
| !!! | TTATATCTAGGTTTTGTTTG+AGG | + | chr1.2:61304651-61304670 | MS.gene70825:intron | 25.0% |
| !!! | TTATTTATCCTTTTGTTTGG+GGG | - | chr1.2:61306034-61306053 | None:intergenic | 25.0% |
| !!! | TTTTAGTTGGATTTTTTCTG+CGG | + | chr1.2:61305915-61305934 | MS.gene70825:intron | 25.0% |
| AAAGAAAAAGACAAGACACT+TGG | + | chr1.2:61305965-61305984 | MS.gene70825:intron | 30.0% | |
| AATGTGAGGTGAAAAAAAGA+GGG | - | chr1.2:61306827-61306846 | None:intergenic | 30.0% | |
| GATGAAGCAGAATCATTTAT+AGG | - | chr1.2:61304409-61304428 | None:intergenic | 30.0% | |
| GCAAATTCTGCCAAAAATTA+TGG | - | chr1.2:61305012-61305031 | None:intergenic | 30.0% | |
| GGTAACTCTTGTACAATTTA+AGG | - | chr1.2:61306612-61306631 | None:intergenic | 30.0% | |
| GTTTGTGATTAAAGATCACA+CGG | + | chr1.2:61304966-61304985 | MS.gene70825:intron | 30.0% | |
| TAATGTGAGGTGAAAAAAAG+AGG | - | chr1.2:61306828-61306847 | None:intergenic | 30.0% | |
| TCACCTGTTAAAAATAGTTG+CGG | + | chr1.2:61304331-61304350 | MS.gene70825:CDS | 30.0% | |
| TGAACATCAACGATCTTAAT+CGG | - | chr1.2:61306250-61306269 | None:intergenic | 30.0% | |
| ! | ATGATCAAAGTGTTATGAGT+TGG | - | chr1.2:61306997-61307016 | None:intergenic | 30.0% |
| ! | ATTGGCAATTTGAGTTTGAT+TGG | + | chr1.2:61305125-61305144 | MS.gene70825:intron | 30.0% |
| ! | GTACAGCTTGATAAATTTTG+AGG | + | chr1.2:61304261-61304280 | MS.gene70825:CDS | 30.0% |
| ! | TAATCTGATGATGAATGGAT+TGG | - | chr1.2:61306906-61306925 | None:intergenic | 30.0% |
| ! | TTCTTTCTAAAGGATCTAAC+TGG | - | chr1.2:61306469-61306488 | None:intergenic | 30.0% |
| ! | TTTGAACATGACAATTTTGG+TGG | + | chr1.2:61305054-61305073 | MS.gene70825:intron | 30.0% |
| !!! | TTTCCGCAACTATTTTTAAC+AGG | - | chr1.2:61304337-61304356 | None:intergenic | 30.0% |
| AAAAATAGTTGCGGAAAGAG+AGG | + | chr1.2:61304340-61304359 | MS.gene70825:CDS | 35.0% | |
| AAGATCACACGGTTACAATT+AGG | + | chr1.2:61304977-61304996 | MS.gene70825:intron | 35.0% | |
| ACAACGTATGGTAATCAATC+AGG | + | chr1.2:61305754-61305773 | MS.gene70825:intron | 35.0% | |
| AGGGGAATCTTATTAGAAAG+AGG | + | chr1.2:61304723-61304742 | MS.gene70825:intron | 35.0% | |
| ATGAAGACAAAGATGGTGAT+TGG | + | chr1.2:61306767-61306786 | MS.gene70825:CDS | 35.0% | |
| ATGTGAGGTGAAAAAAAGAG+GGG | - | chr1.2:61306826-61306845 | None:intergenic | 35.0% | |
| ATTTGTGTGCAACACTATTG+AGG | + | chr1.2:61304703-61304722 | MS.gene70825:intron | 35.0% | |
| CAACGTATGGTAATCAATCA+GGG | + | chr1.2:61305755-61305774 | MS.gene70825:intron | 35.0% | |
| CATAATCAGAACCGTTCAAA+AGG | - | chr1.2:61306738-61306757 | None:intergenic | 35.0% | |
| CCAACATATGAAGACAAAGA+TGG | + | chr1.2:61306760-61306779 | MS.gene70825:CDS | 35.0% | |
| CCATCTTTGTCTTCATATGT+TGG | - | chr1.2:61306763-61306782 | None:intergenic | 35.0% | |
| CCTTCACAATTGACAATTGT+GGG | + | chr1.2:61306668-61306687 | MS.gene70825:CDS | 35.0% | |
| GAATAAACATGGACAACGTA+TGG | + | chr1.2:61305742-61305761 | MS.gene70825:intron | 35.0% | |
| GCTTTCATTCATAAAGTCCT+TGG | - | chr1.2:61306704-61306723 | None:intergenic | 35.0% | |
| TCAATGACGATAGAGAATCA+TGG | + | chr1.2:61306300-61306319 | MS.gene70825:intron | 35.0% | |
| TCCTTCACAATTGACAATTG+TGG | + | chr1.2:61306667-61306686 | MS.gene70825:CDS | 35.0% | |
| TGTTAATTCCCCCAAACAAA+AGG | + | chr1.2:61306023-61306042 | MS.gene70825:intron | 35.0% | |
| TTTGTGTGCAACACTATTGA+GGG | + | chr1.2:61304704-61304723 | MS.gene70825:intron | 35.0% | |
| ! | CAAAGTGTTATGAGTTGGAT+TGG | - | chr1.2:61306992-61307011 | None:intergenic | 35.0% |
| ! | CATAAAGTCCTTGGTAACTT+GGG | - | chr1.2:61306695-61306714 | None:intergenic | 35.0% |
| ! | CGACTTAATCTGATGATGAA+TGG | - | chr1.2:61306911-61306930 | None:intergenic | 35.0% |
| ! | TCATAAAGTCCTTGGTAACT+TGG | - | chr1.2:61306696-61306715 | None:intergenic | 35.0% |
| ! | TGTTATGAGTTGGATTGGAT+TGG | - | chr1.2:61306987-61307006 | None:intergenic | 35.0% |
| ! | TTTCTAAAGGATCTAACTGG+TGG | - | chr1.2:61306466-61306485 | None:intergenic | 35.0% |
| !! | TGATGATGAATGGATTGGTT+CGG | - | chr1.2:61306901-61306920 | None:intergenic | 35.0% |
| !! | TTGCATTTTTCAACTGCTCT+TGG | - | chr1.2:61307237-61307256 | None:intergenic | 35.0% |
| AAGCGTCTTCGCATAATGAA+AGG | + | chr1.2:61307082-61307101 | MS.gene70825:CDS | 40.0% | |
| AATTTGTAGGGCACAAGTTG+TGG | + | chr1.2:61306435-61306454 | MS.gene70825:intron | 40.0% | |
| AGAAAAACTCACTTAGCACG+AGG | - | chr1.2:61304505-61304524 | None:intergenic | 40.0% | |
| ATTTGTAGGGCACAAGTTGT+GGG | + | chr1.2:61306436-61306455 | MS.gene70825:intron | 40.0% | |
| CATCAACGATCTTAATCGGA+TGG | - | chr1.2:61306246-61306265 | None:intergenic | 40.0% | |
| CCCACAATTGTCAATTGTGA+AGG | - | chr1.2:61306671-61306690 | None:intergenic | 40.0% | |
| GCTTCTGTAGCTGAAAACAA+AGG | + | chr1.2:61304442-61304461 | MS.gene70825:CDS | 40.0% | |
| TATGGTAATCAATCAGGGCA+TGG | + | chr1.2:61305760-61305779 | MS.gene70825:intron | 40.0% | |
| TTGTGTGCAACACTATTGAG+GGG | + | chr1.2:61304705-61304724 | MS.gene70825:intron | 40.0% | |
| TTTGTTAAGGTGAGCATGGA+TGG | + | chr1.2:61306562-61306581 | MS.gene70825:CDS | 40.0% | |
| ! | AGCATTTGTTAAGGTGAGCA+TGG | + | chr1.2:61306558-61306577 | MS.gene70825:CDS | 40.0% |
| ! | TGGGAATGGAGCATTTGTTA+AGG | + | chr1.2:61306549-61306568 | MS.gene70825:CDS | 40.0% |
| !! | GATGATGAATGGATTGGTTC+GGG | - | chr1.2:61306900-61306919 | None:intergenic | 40.0% |
| !!! | GATTTTTTCTGCGGATAGCA+TGG | + | chr1.2:61305924-61305943 | MS.gene70825:intron | 40.0% |
| !!! | GTTTTGTTTGAGGCTGAGAT+AGG | + | chr1.2:61304661-61304680 | MS.gene70825:intron | 40.0% |
| AAAACTCACTTAGCACGAGG+AGG | - | chr1.2:61304502-61304521 | None:intergenic | 45.0% | |
| AGGTCCACCTTACGTAGATA+TGG | - | chr1.2:61306592-61306611 | None:intergenic | 45.0% | |
| ATGAAAGGTTCCGAGGCAAT+TGG | + | chr1.2:61307097-61307116 | MS.gene70825:CDS | 45.0% | |
| CAGTCGATGGTAGAGAATCT+TGG | + | chr1.2:61306352-61306371 | MS.gene70825:intron | 45.0% | |
| GACTGCATGAATTTACGCAG+TGG | - | chr1.2:61306282-61306301 | None:intergenic | 45.0% | |
| GATGGTGATTGGATGCTTGT+TGG | + | chr1.2:61306778-61306797 | MS.gene70825:CDS | 45.0% | |
| GCATAAATTCGTGCAGTCGA+TGG | + | chr1.2:61306339-61306358 | MS.gene70825:intron | 45.0% | |
| TCGCATAATGAAAGGTTCCG+AGG | + | chr1.2:61307090-61307109 | MS.gene70825:CDS | 45.0% | |
| TGAAAGGTTCCGAGGCAATT+GGG | + | chr1.2:61307098-61307117 | MS.gene70825:CDS | 45.0% | |
| TGCACCATATCTACGTAAGG+TGG | + | chr1.2:61306585-61306604 | MS.gene70825:CDS | 45.0% | |
| TGGTGCACCATATCTACGTA+AGG | + | chr1.2:61306582-61306601 | MS.gene70825:CDS | 45.0% | |
| TTTACCTAGCCCAATTGCCT+CGG | - | chr1.2:61307110-61307129 | None:intergenic | 45.0% | |
| ! | ATAAGAGCACAAGTGGTGGT+GGG | + | chr1.2:61306530-61306549 | MS.gene70825:CDS | 45.0% |
| ! | GAAGCAGATAAGAGCACAAG+TGG | + | chr1.2:61306523-61306542 | MS.gene70825:CDS | 45.0% |
| ! | TTTTCTGCGGATAGCATGGT+TGG | + | chr1.2:61305928-61305947 | MS.gene70825:intron | 45.0% |
| !! | AAGTATTGGATGGTGGCTGA+AGG | + | chr1.2:61305861-61305880 | MS.gene70825:intron | 45.0% |
| !! | ATTGGTTCGGGTCAGATCAA+CGG | - | chr1.2:61306888-61306907 | None:intergenic | 45.0% |
| !! | GCTTGTTGGTGATGTTCCTT+GGG | + | chr1.2:61306792-61306811 | MS.gene70825:CDS | 45.0% |
| !! | TGCTTGTTGGTGATGTTCCT+TGG | + | chr1.2:61306791-61306810 | MS.gene70825:CDS | 45.0% |
| !! | AATAAAAAAAAATAAAGTAT+TGG | + | chr1.2:61305847-61305866 | MS.gene70825:intron | 5.0% |
| AATCAATCAGGGCATGGTGG+AGG | + | chr1.2:61305766-61305785 | MS.gene70825:intron | 50.0% | |
| CTTCCAGGTGCATGTGATCA+CGG | + | chr1.2:61304304-61304323 | MS.gene70825:CDS | 50.0% | |
| GGTAATCAATCAGGGCATGG+TGG | + | chr1.2:61305763-61305782 | MS.gene70825:intron | 50.0% | |
| GTGACACGCACACAAGACAA+GGG | - | chr1.2:61306080-61306099 | None:intergenic | 50.0% | |
| TGGAAGACCGAGTCGTAACT+CGG | - | chr1.2:61304290-61304309 | None:intergenic | 50.0% | |
| TGGTGGCTGAAGGAATAGGT+TGG | + | chr1.2:61305871-61305890 | MS.gene70825:intron | 50.0% | |
| TTGTGGGTCCCAAGTTACCA+AGG | + | chr1.2:61306684-61306703 | MS.gene70825:CDS | 50.0% | |
| ! | CGGATAGCATGGTTGGTTGT+CGG | + | chr1.2:61305935-61305954 | MS.gene70825:intron | 50.0% |
| ! | GATAAGAGCACAAGTGGTGG+TGG | + | chr1.2:61306529-61306548 | MS.gene70825:CDS | 50.0% |
| ! | GCAGATAAGAGCACAAGTGG+TGG | + | chr1.2:61306526-61306545 | MS.gene70825:CDS | 50.0% |
| ! | GGATAGCATGGTTGGTTGTC+GGG | + | chr1.2:61305936-61305955 | MS.gene70825:intron | 50.0% |
| ! | TGGATGGTGGCTGAAGGAAT+AGG | + | chr1.2:61305867-61305886 | MS.gene70825:intron | 50.0% |
| AAGAGGGGAACTCACTCCCA+AGG | - | chr1.2:61306811-61306830 | None:intergenic | 55.0% | |
| ACTCACTTAGCACGAGGAGG+AGG | - | chr1.2:61304499-61304518 | None:intergenic | 55.0% | |
| GAGGAGACCGAGTTACGACT+CGG | + | chr1.2:61304280-61304299 | MS.gene70825:CDS | 55.0% | |
| GGTGACACGCACACAAGACA+AGG | - | chr1.2:61306081-61306100 | None:intergenic | 55.0% | |
| GTAGGGCACAAGTTGTGGGA+TGG | + | chr1.2:61306440-61306459 | MS.gene70825:intron | 55.0% | |
| TCTCCGTGATCACATGCACC+TGG | - | chr1.2:61304310-61304329 | None:intergenic | 55.0% | |
| !! | AGCACAAGTGGTGGTGGGAA+TGG | + | chr1.2:61306535-61306554 | MS.gene70825:CDS | 55.0% |
| !! | GAGTTACGACTCGGTCTTCC+AGG | + | chr1.2:61304289-61304308 | MS.gene70825:CDS | 55.0% |
| CCAAGAGCTGTCAGATGCGC+TGG | + | chr1.2:61306630-61306649 | MS.gene70825:CDS | 60.0% | |
| CCAGCGCATCTGACAGCTCT+TGG | - | chr1.2:61306633-61306652 | None:intergenic | 60.0% | |
| GGTTCCGAGGCAATTGGGCT+AGG | + | chr1.2:61307103-61307122 | MS.gene70825:CDS | 60.0% | |
| ! | GCACACAAGACAAGGGCGTG+TGG | - | chr1.2:61306073-61306092 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 61304229 | 61307269 | 61304229 | ID=MS.gene70825 |
| chr1.2 | mRNA | 61304229 | 61307269 | 61304229 | ID=MS.gene70825.t1;Parent=MS.gene70825 |
| chr1.2 | exon | 61304229 | 61304512 | 61304229 | ID=MS.gene70825.t1.exon1;Parent=MS.gene70825.t1 |
| chr1.2 | CDS | 61304229 | 61304512 | 61304229 | ID=cds.MS.gene70825.t1;Parent=MS.gene70825.t1 |
| chr1.2 | exon | 61306444 | 61306815 | 61306444 | ID=MS.gene70825.t1.exon2;Parent=MS.gene70825.t1 |
| chr1.2 | CDS | 61306444 | 61306815 | 61306444 | ID=cds.MS.gene70825.t1;Parent=MS.gene70825.t1 |
| chr1.2 | exon | 61307063 | 61307124 | 61307063 | ID=MS.gene70825.t1.exon3;Parent=MS.gene70825.t1 |
| chr1.2 | CDS | 61307063 | 61307124 | 61307063 | ID=cds.MS.gene70825.t1;Parent=MS.gene70825.t1 |
| chr1.2 | exon | 61307232 | 61307269 | 61307232 | ID=MS.gene70825.t1.exon4;Parent=MS.gene70825.t1 |
| chr1.2 | CDS | 61307232 | 61307269 | 61307232 | ID=cds.MS.gene70825.t1;Parent=MS.gene70825.t1 |
| Gene Sequence |
| Protein sequence |
