AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72157


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72157.t1 AT5G22250 64.662 266 90 3 9 270 12 277 6.40e-120 344
MS.gene72157.t1 AT3G44260 61.132 265 98 3 9 270 17 279 4.51e-116 335
MS.gene72157.t1 AT2G32070 50.746 268 127 5 4 270 5 268 4.63e-92 273
MS.gene72157.t1 AT5G10960 50.562 267 128 4 4 270 5 267 7.14e-91 270
MS.gene72157.t1 AT1G80780 49.064 267 132 4 4 270 5 267 1.27e-88 265
MS.gene72157.t1 AT1G80780 49.064 267 132 4 4 270 5 267 1.27e-88 265
MS.gene72157.t1 AT1G80780 49.064 267 132 4 4 270 5 267 1.73e-88 265
MS.gene72157.t1 AT1G15920 47.407 270 134 5 5 269 10 276 8.64e-81 245
MS.gene72157.t1 AT1G15920 47.407 270 134 5 5 269 10 276 8.64e-81 245
MS.gene72157.t1 AT1G61470 37.037 243 130 7 13 249 4 229 1.14e-38 137
MS.gene72157.t1 AT1G06450 33.700 273 157 9 1 268 1 254 1.51e-38 138
MS.gene72157.t1 AT1G27820 35.510 245 143 5 7 248 2 234 8.44e-34 125
MS.gene72157.t1 AT1G27890 35.565 239 139 5 13 248 3 229 1.60e-32 121
MS.gene72157.t1 AT3G44240 35.242 227 132 7 25 249 3 216 3.77e-32 119

Find 62 sgRNAs with CRISPR-Local

Find 70 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCACAGCGCTTATGATTT+TGG 0.104590 4.1:-6089661 MS.gene72157:CDS
ATCAAATTCAACCTCTAAAT+TGG 0.207989 4.1:+6090092 None:intergenic
GATGGCAACCTTCCTCATTT+TGG 0.210731 4.1:-6089889 MS.gene72157:CDS
AAACACGCTGGGGTACTATT+TGG 0.216392 4.1:-6089364 MS.gene72157:CDS
GCTGAATTGATGCTCACTTC+TGG 0.258268 4.1:-6089718 MS.gene72157:CDS
CAATCATCATCCGTCAAGTT+TGG 0.286714 4.1:-6090119 MS.gene72157:CDS
TCTTCAACAAATCAGTTGTT+TGG 0.295934 4.1:-6089690 MS.gene72157:CDS
AACGGTTCGGCAGATTCCTT+CGG 0.352418 4.1:+6089617 None:intergenic
CTTAACGTGGGTCGGGTTGC+TGG 0.360050 4.1:-6089478 MS.gene72157:CDS
AGATGACGGCGGTCAACTTT+GGG 0.368577 4.1:+6089997 None:intergenic
CTTCAACAAATCAGTTGTTT+GGG 0.376564 4.1:-6089689 MS.gene72157:CDS
GGACAAATAGCCGCTACATA+TGG 0.395638 4.1:-6089867 MS.gene72157:CDS
ACGGTTCGGCAGATTCCTTC+GGG 0.395658 4.1:+6089618 None:intergenic
AATCATCATCCGTCAAGTTT+GGG 0.414340 4.1:-6090118 MS.gene72157:CDS
AAGATGACGGCGGTCAACTT+TGG 0.418710 4.1:+6089996 None:intergenic
GAATCTGCCGAACCGTTTAG+AGG 0.420121 4.1:-6089611 MS.gene72157:CDS
AGTTTGGGCATCCAATTTAG+AGG 0.424325 4.1:-6090103 MS.gene72157:CDS
TCCATGGATACTGAATTTCC+AGG 0.437255 4.1:-6090033 MS.gene72157:CDS
GGTTGCCATCACCATCAGAT+AGG 0.449581 4.1:+6089902 None:intergenic
CAAACTTGACGGATGATGAT+TGG 0.450788 4.1:+6090120 None:intergenic
TACTCATTTCTTGAATAATG+AGG 0.477666 4.1:-6089392 MS.gene72157:CDS
TGACCACTATCGTTACTTGA+AGG 0.492905 4.1:-6089965 MS.gene72157:CDS
GACAAATAGCCGCTACATAT+GGG 0.500452 4.1:-6089866 MS.gene72157:CDS
CAATTGAGCCTTGGTTGTAA+AGG 0.504025 4.1:+6089809 None:intergenic
TGCGCTTGAAGTCAATCCCT+TGG 0.505546 4.1:+6089773 None:intergenic
TCAATATCCTTTCATCTCCA+TGG 0.506697 4.1:-6090049 MS.gene72157:CDS
TACAACACTTAACGTGGGTC+GGG 0.508105 4.1:-6089485 MS.gene72157:CDS
AATTGGATGCCCAAACTTGA+CGG 0.510297 4.1:+6090109 None:intergenic
ACCTGGAAATTCAGTATCCA+TGG 0.514112 4.1:+6090032 None:intergenic
AGGTTCTGCAACGCTTTGTA+CGG 0.515634 4.1:-6089526 MS.gene72157:CDS
TTCAAGCGCAATTTATCTCA+TGG 0.520248 4.1:-6089760 MS.gene72157:CDS
CAGATAGGGTGATACCAACT+TGG 0.523809 4.1:+6089917 None:intergenic
GATATTGATTGACAACTTGA+CGG 0.523978 4.1:+6090064 None:intergenic
GCATGCATTTAAGAAAATGA+TGG 0.528476 4.1:-6089416 MS.gene72157:CDS
CTACAACACTTAACGTGGGT+CGG 0.533742 4.1:-6089486 MS.gene72157:CDS
CGGCTATTTGTCCCAAAATG+AGG 0.540450 4.1:+6089877 None:intergenic
GCTGGAAAGTCTCATCAAGC+TGG 0.542113 4.1:-6089460 MS.gene72157:CDS
ATTGACATGCTCTGCCGCCA+AGG 0.544877 4.1:-6089790 MS.gene72157:CDS
AGAGCATGTCAATTGAGCCT+TGG 0.548111 4.1:+6089800 None:intergenic
TTGACATGCTCTGCCGCCAA+GGG 0.557345 4.1:-6089789 MS.gene72157:CDS
GCTTGAAGTCAATCCCTTGG+CGG 0.562753 4.1:+6089776 None:intergenic
GCTCTTAAACTCATCCAAGT+TGG 0.568904 4.1:-6089931 MS.gene72157:CDS
ATGATATGAAACACATGATG+AGG 0.569362 4.1:-6089546 MS.gene72157:CDS
TTGGGTGAATAAATTACACC+TGG 0.570783 4.1:+6090015 None:intergenic
GTTGCCATCACCATCAGATA+GGG 0.583084 4.1:+6089903 None:intergenic
TTCTGCAACGCTTTGTACGG+TGG 0.584961 4.1:-6089523 MS.gene72157:CDS
ATTCAAAATGAACCAGCTGA+AGG 0.587598 4.1:-6090148 None:intergenic
GGTCAGAGGGTTTAAGATGA+CGG 0.592880 4.1:+6089983 None:intergenic
AAGTAACGATAGTGGTCAGA+GGG 0.593594 4.1:+6089970 None:intergenic
TAGTGAAGATATTGACCCGA+AGG 0.595020 4.1:-6089633 MS.gene72157:CDS
TCAGTATCCATGGAGATGAA+AGG 0.598752 4.1:+6090042 None:intergenic
GTTGGTATCACCCTATCTGA+TGG 0.609828 4.1:-6089913 MS.gene72157:CDS
TGACCTTCAAGTAACGATAG+TGG 0.622378 4.1:+6089962 None:intergenic
TATTTGTCCCAAAATGAGGA+AGG 0.623706 4.1:+6089881 None:intergenic
AGTCGCTACAACACTTAACG+TGG 0.625698 4.1:-6089491 MS.gene72157:CDS
CAAGTAACGATAGTGGTCAG+AGG 0.631701 4.1:+6089969 None:intergenic
GAGCATGACCTTTACAACCA+AGG 0.646186 4.1:-6089817 MS.gene72157:CDS
AGTTGAATTCCCATATGTAG+CGG 0.649610 4.1:+6089857 None:intergenic
GATCAGATAGTTTGTTGACG+TGG 0.660381 4.1:-6089438 MS.gene72157:CDS
ATCACCCTATCTGATGGTGA+TGG 0.683961 4.1:-6089907 MS.gene72157:CDS
GTCGCTACAACACTTAACGT+GGG 0.701411 4.1:-6089490 MS.gene72157:CDS
CAGAGGGTTTAAGATGACGG+CGG 0.736962 4.1:+6089986 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAAAAAGTCCTCTAAA+CGG + chr4.1:6089890-6089909 None:intergenic 20.0%
! ATCAAATTCAACCTCTAAAT+TGG + chr4.1:6089397-6089416 None:intergenic 25.0%
! TACTCATTTCTTGAATAATG+AGG - chr4.1:6090094-6090113 MS.gene72157:CDS 25.0%
ATGATATGAAACACATGATG+AGG - chr4.1:6089940-6089959 MS.gene72157:CDS 30.0%
CTTCAACAAATCAGTTGTTT+GGG - chr4.1:6089797-6089816 MS.gene72157:CDS 30.0%
GCATGCATTTAAGAAAATGA+TGG - chr4.1:6090070-6090089 MS.gene72157:CDS 30.0%
TCTTCAACAAATCAGTTGTT+TGG - chr4.1:6089796-6089815 MS.gene72157:CDS 30.0%
! GATATTGATTGACAACTTGA+CGG + chr4.1:6089425-6089444 None:intergenic 30.0%
AAAAAGTCCTCTAAACGGTT+CGG + chr4.1:6089885-6089904 None:intergenic 35.0%
AATCATCATCCGTCAAGTTT+GGG - chr4.1:6089368-6089387 MS.gene72157:CDS 35.0%
AGTTGAATTCCCATATGTAG+CGG + chr4.1:6089632-6089651 None:intergenic 35.0%
TATTTGTCCCAAAATGAGGA+AGG + chr4.1:6089608-6089627 None:intergenic 35.0%
TCAATATCCTTTCATCTCCA+TGG - chr4.1:6089437-6089456 MS.gene72157:CDS 35.0%
TTCAAGCGCAATTTATCTCA+TGG - chr4.1:6089726-6089745 MS.gene72157:CDS 35.0%
TTGGGTGAATAAATTACACC+TGG + chr4.1:6089474-6089493 None:intergenic 35.0%
! TTCACAGCGCTTATGATTTT+GGG - chr4.1:6089826-6089845 MS.gene72157:CDS 35.0%
! TTTCACAGCGCTTATGATTT+TGG - chr4.1:6089825-6089844 MS.gene72157:CDS 35.0%
AATTGGATGCCCAAACTTGA+CGG + chr4.1:6089380-6089399 None:intergenic 40.0%
ACCTGGAAATTCAGTATCCA+TGG + chr4.1:6089457-6089476 None:intergenic 40.0%
AGTTTGGGCATCCAATTTAG+AGG - chr4.1:6089383-6089402 MS.gene72157:CDS 40.0%
CAATCATCATCCGTCAAGTT+TGG - chr4.1:6089367-6089386 MS.gene72157:CDS 40.0%
CAATTGAGCCTTGGTTGTAA+AGG + chr4.1:6089680-6089699 None:intergenic 40.0%
GACAAATAGCCGCTACATAT+GGG - chr4.1:6089620-6089639 MS.gene72157:CDS 40.0%
GCTCTTAAACTCATCCAAGT+TGG - chr4.1:6089555-6089574 MS.gene72157:CDS 40.0%
TAGTGAAGATATTGACCCGA+AGG - chr4.1:6089853-6089872 MS.gene72157:CDS 40.0%
TCAGTATCCATGGAGATGAA+AGG + chr4.1:6089447-6089466 None:intergenic 40.0%
TCCATGGATACTGAATTTCC+AGG - chr4.1:6089453-6089472 MS.gene72157:CDS 40.0%
TGACCACTATCGTTACTTGA+AGG - chr4.1:6089521-6089540 MS.gene72157:CDS 40.0%
TGACCTTCAAGTAACGATAG+TGG + chr4.1:6089527-6089546 None:intergenic 40.0%
! AAGTAACGATAGTGGTCAGA+GGG + chr4.1:6089519-6089538 None:intergenic 40.0%
! ATGGCAACCTTCCTCATTTT+GGG - chr4.1:6089598-6089617 MS.gene72157:CDS 40.0%
! CAAACTTGACGGATGATGAT+TGG + chr4.1:6089369-6089388 None:intergenic 40.0%
!! GATCAGATAGTTTGTTGACG+TGG - chr4.1:6090048-6090067 MS.gene72157:CDS 40.0%
AATGAGGCTCAAAAACACGC+TGG - chr4.1:6090110-6090129 MS.gene72157:CDS 45.0%
AGAGCATGTCAATTGAGCCT+TGG + chr4.1:6089689-6089708 None:intergenic 45.0%
AGGTTCTGCAACGCTTTGTA+CGG - chr4.1:6089960-6089979 MS.gene72157:CDS 45.0%
AGTCGCTACAACACTTAACG+TGG - chr4.1:6089995-6090014 MS.gene72157:CDS 45.0%
ATGAGGCTCAAAAACACGCT+GGG - chr4.1:6090111-6090130 MS.gene72157:CDS 45.0%
CAGATAGGGTGATACCAACT+TGG + chr4.1:6089572-6089591 None:intergenic 45.0%
CGGCTATTTGTCCCAAAATG+AGG + chr4.1:6089612-6089631 None:intergenic 45.0%
CTACAACACTTAACGTGGGT+CGG - chr4.1:6090000-6090019 MS.gene72157:CDS 45.0%
GAGCATGACCTTTACAACCA+AGG - chr4.1:6089669-6089688 MS.gene72157:CDS 45.0%
GCTGAATTGATGCTCACTTC+TGG - chr4.1:6089768-6089787 MS.gene72157:CDS 45.0%
GGACAAATAGCCGCTACATA+TGG - chr4.1:6089619-6089638 MS.gene72157:CDS 45.0%
GTCGCTACAACACTTAACGT+GGG - chr4.1:6089996-6090015 MS.gene72157:CDS 45.0%
GTTGCCATCACCATCAGATA+GGG + chr4.1:6089586-6089605 None:intergenic 45.0%
GTTGGTATCACCCTATCTGA+TGG - chr4.1:6089573-6089592 MS.gene72157:CDS 45.0%
TACAACACTTAACGTGGGTC+GGG - chr4.1:6090001-6090020 MS.gene72157:CDS 45.0%
! AAACACGCTGGGGTACTATT+TGG - chr4.1:6090122-6090141 MS.gene72157:CDS 45.0%
! ATCACCCTATCTGATGGTGA+TGG - chr4.1:6089579-6089598 MS.gene72157:CDS 45.0%
! CAAGTAACGATAGTGGTCAG+AGG + chr4.1:6089520-6089539 None:intergenic 45.0%
! GATGGCAACCTTCCTCATTT+TGG - chr4.1:6089597-6089616 MS.gene72157:CDS 45.0%
! GGTCAGAGGGTTTAAGATGA+CGG + chr4.1:6089506-6089525 None:intergenic 45.0%
!!! TGATTGGCTTTTCCTTCAGC+TGG + chr4.1:6089353-6089372 None:intergenic 45.0%
!!! AATATTTTAACAATATTATT+TGG - chr4.1:6089906-6089925 MS.gene72157:CDS 5.0%
GAATCTGCCGAACCGTTTAG+AGG - chr4.1:6089875-6089894 MS.gene72157:CDS 50.0%
GCTGGAAAGTCTCATCAAGC+TGG - chr4.1:6090026-6090045 MS.gene72157:CDS 50.0%
GCTTGAAGTCAATCCCTTGG+CGG + chr4.1:6089713-6089732 None:intergenic 50.0%
GGTTGCCATCACCATCAGAT+AGG + chr4.1:6089587-6089606 None:intergenic 50.0%
TGAGGCTCAAAAACACGCTG+GGG - chr4.1:6090112-6090131 MS.gene72157:CDS 50.0%
TGCGCTTGAAGTCAATCCCT+TGG + chr4.1:6089716-6089735 None:intergenic 50.0%
TTCTGCAACGCTTTGTACGG+TGG - chr4.1:6089963-6089982 MS.gene72157:CDS 50.0%
! AACGGTTCGGCAGATTCCTT+CGG + chr4.1:6089872-6089891 None:intergenic 50.0%
! AAGATGACGGCGGTCAACTT+TGG + chr4.1:6089493-6089512 None:intergenic 50.0%
! AGATGACGGCGGTCAACTTT+GGG + chr4.1:6089492-6089511 None:intergenic 50.0%
! CAGAGGGTTTAAGATGACGG+CGG + chr4.1:6089503-6089522 None:intergenic 50.0%
ATTGACATGCTCTGCCGCCA+AGG - chr4.1:6089696-6089715 MS.gene72157:CDS 55.0%
TTGACATGCTCTGCCGCCAA+GGG - chr4.1:6089697-6089716 MS.gene72157:CDS 55.0%
! ACGGTTCGGCAGATTCCTTC+GGG + chr4.1:6089871-6089890 None:intergenic 55.0%
! CTTAACGTGGGTCGGGTTGC+TGG - chr4.1:6090008-6090027 MS.gene72157:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 6089345 6090163 6089345 ID=MS.gene72157
chr4.1 mRNA 6089345 6090163 6089345 ID=MS.gene72157.t1;Parent=MS.gene72157
chr4.1 exon 6089345 6090163 6089345 ID=MS.gene72157.t1.exon1;Parent=MS.gene72157.t1
chr4.1 CDS 6089345 6090163 6089345 ID=cds.MS.gene72157.t1;Parent=MS.gene72157.t1
Gene Sequence

>MS.gene72157

ATGAACCAGCTGAAGGAAAAGCCAATCATCATCCGTCAAGTTTGGGCATCCAATTTAGAGGTTGAATTTGATCTCATCCGTCAAGTTGTCAATCAATATCCTTTCATCTCCATGGATACTGAATTTCCAGGTGTAATTTATTCACCCAAAGTTGACCGCCGTCATCTTAAACCCTCTGACCACTATCGTTACTTGAAGGTCAACGTTGATGCTCTTAAACTCATCCAAGTTGGTATCACCCTATCTGATGGTGATGGCAACCTTCCTCATTTTGGGACAAATAGCCGCTACATATGGGAATTCAACTTTTGCGATTTTGATTTTGAGCATGACCTTTACAACCAAGGCTCAATTGACATGCTCTGCCGCCAAGGGATTGACTTCAAGCGCAATTTATCTCATGGTGTGAATTCGTCACGTTTTGCTGAATTGATGCTCACTTCTGGACTTGTCTTCAACAAATCAGTTGTTTGGGTCACATTTCACAGCGCTTATGATTTTGGGTATTTAGTGAAGATATTGACCCGAAGGAATCTGCCGAACCGTTTAGAGGACTTTTTAAATATTTTAACAATATTATTTGGAAAGAATGTTTATGATATGAAACACATGATGAGGTTCTGCAACGCTTTGTACGGTGGTCTCGAGCGAGTCGCTACAACACTTAACGTGGGTCGGGTTGCTGGAAAGTCTCATCAAGCTGGATCAGATAGTTTGTTGACGTGGCATGCATTTAAGAAAATGATGGATACTCATTTCTTGAATAATGAGGCTCAAAAACACGCTGGGGTACTATTTGGATTAGAAATTGCAACTTAG

Protein sequence

>MS.gene72157.t1

MNQLKEKPIIIRQVWASNLEVEFDLIRQVVNQYPFISMDTEFPGVIYSPKVDRRHLKPSDHYRYLKVNVDALKLIQVGITLSDGDGNLPHFGTNSRYIWEFNFCDFDFEHDLYNQGSIDMLCRQGIDFKRNLSHGVNSSRFAELMLTSGLVFNKSVVWVTFHSAYDFGYLVKILTRRNLPNRLEDFLNILTILFGKNVYDMKHMMRFCNALYGGLERVATTLNVGRVAGKSHQAGSDSLLTWHAFKKMMDTHFLNNEAQKHAGVLFGLEIAT