AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene72621


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72621.t1 MTR_6g070920 91.824 159 1 1 1 147 72 230 4.45e-105 299
MS.gene72621.t1 MTR_6g070920 87.407 135 5 1 1 123 72 206 5.16e-83 243
MS.gene72621.t1 MTR_3g065330 55.696 158 53 4 1 142 10 166 3.17e-54 169
MS.gene72621.t1 MTR_1g032290 54.717 159 56 4 1 144 1 158 8.86e-54 167
MS.gene72621.t1 MTR_8g105140 54.037 161 58 4 1 146 1 160 2.06e-53 166
MS.gene72621.t1 MTR_5g078020 51.656 151 55 3 8 142 16 164 5.82e-46 148
MS.gene72621.t1 MTR_6g070920 81.319 91 5 1 1 79 72 162 1.46e-45 146
MS.gene72621.t1 MTR_3g065330 55.556 135 43 4 1 119 32 165 4.65e-43 140
MS.gene72621.t1 MTR_8g039690 41.509 159 72 3 2 139 1 159 1.78e-33 116
MS.gene72621.t1 MTR_7g084380 42.331 163 70 4 2 140 1 163 1.80e-32 114
MS.gene72621.t1 MTR_7g084380 40.288 139 59 4 26 140 21 159 2.40e-23 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene72621.t1 AT1G18300 55.414 157 53 4 1 141 40 195 2.26e-55 172
MS.gene72621.t1 AT1G73540 55.414 157 53 4 1 143 41 194 3.93e-53 167
MS.gene72621.t1 AT1G14860 55.128 156 55 3 1 142 1 155 8.93e-53 165
MS.gene72621.t1 AT2G01670 50.932 161 64 3 1 147 6 165 3.50e-50 159
MS.gene72621.t1 AT1G12880 42.169 166 69 4 2 140 1 166 5.44e-33 115
MS.gene72621.t1 AT3G26690 40.120 167 72 4 2 140 1 167 8.81e-30 107
MS.gene72621.t1 AT3G26690 40.120 167 72 4 2 140 1 167 8.81e-30 107
MS.gene72621.t1 AT3G12600 43.750 160 67 4 2 138 1 160 1.86e-26 98.2
MS.gene72621.t1 AT3G12600 43.243 148 61 4 14 138 4 151 1.81e-22 87.8

Find 33 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCTTATGTTCATTTC+TGG 0.092026 6.4:+48421348 None:intergenic
TGCAGAGTAATTTGGGTAAA+TGG 0.215433 6.4:-48421452 MS.gene72621:intron
TGAACATAAGAAGAAGAAAA+TGG 0.253808 6.4:-48421341 MS.gene72621:CDS
ATAGAAGAAGCTGGAGTTAT+TGG 0.256499 6.4:-48421587 MS.gene72621:CDS
AATAACTCCAGCTTCTTCTA+TGG 0.282235 6.4:+48421589 None:intergenic
TTAGCAAGGAGTTGGATAAT+TGG 0.322855 6.4:-48421371 MS.gene72621:CDS
GAAGGAAGCTTTAGATGAAT+TGG 0.336584 6.4:-48420935 MS.gene72621:CDS
ACCTAAAACTTAGATTGAAT+TGG 0.341917 6.4:+48420900 None:intergenic
TGCAAATGTCTACCAGTTCT+AGG 0.360990 6.4:+48422262 None:intergenic
GCTTCCTTCATCCAAGCATA+TGG 0.390570 6.4:+48420949 None:intergenic
AAGAAATATGCCCATATGCT+TGG 0.435806 6.4:-48420960 MS.gene72621:CDS
TGTTTGGTTTCTCCTAGAAC+TGG 0.445974 6.4:-48422274 MS.gene72621:CDS
ATTGGAGAATATGATGTGTT+TGG 0.449206 6.4:-48422290 None:intergenic
GAACATAAGAAGAAGAAAAT+GGG 0.454169 6.4:-48421340 MS.gene72621:intron
GTTCCCTTTGCTTGTTAGCA+AGG 0.472162 6.4:-48421385 MS.gene72621:CDS
TTTGCTTGTTAGCAAGGAGT+TGG 0.481309 6.4:-48421379 MS.gene72621:CDS
AAAGAATGGAACTCAAGAAA+AGG 0.494824 6.4:-48421717 MS.gene72621:CDS
TCTCAAAGCAGCTTGTTCCA+TGG 0.496073 6.4:+48421616 None:intergenic
AGAGAAACCATAGAAGAAGC+TGG 0.505150 6.4:-48421596 MS.gene72621:CDS
AACTCAAGAAAAGGAAATAG+AGG 0.509278 6.4:-48421708 MS.gene72621:CDS
AACTCCTTGCTAACAAGCAA+AGG 0.516565 6.4:+48421381 None:intergenic
CTTCCTTCATCCAAGCATAT+GGG 0.522789 6.4:+48420950 None:intergenic
ACAAGGATGTCGTCAAGTTG+TGG 0.541451 6.4:-48422230 MS.gene72621:CDS
CATGTATCCTTCATGCATAG+TGG 0.542137 6.4:+48421407 None:intergenic
CAAGGATGTCGTCAAGTTGT+GGG 0.550727 6.4:-48422229 MS.gene72621:CDS
ACTCCTTGCTAACAAGCAAA+GGG 0.551729 6.4:+48421382 None:intergenic
ATGCCCATATGCTTGGATGA+AGG 0.557447 6.4:-48420953 MS.gene72621:CDS
ATCCTTCATGCATAGTGGGT+TGG 0.562217 6.4:+48421412 None:intergenic
ATGTATCCTTCATGCATAGT+GGG 0.569535 6.4:+48421408 None:intergenic
CATTTGCAACGTTATGAACA+AGG 0.571762 6.4:-48422247 MS.gene72621:CDS
CGCCAACCCACTATGCATGA+AGG 0.576057 6.4:-48421414 MS.gene72621:CDS
AAGGATGTCGTCAAGTTGTG+GGG 0.655005 6.4:-48422228 MS.gene72621:intron
TGTAATCATGCAGTTGACTG+TGG 0.660929 6.4:-48420992 MS.gene72621:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATAAAAGTTGAATAT+AGG + chr6.4:48421890-48421909 None:intergenic 10.0%
!!! ATGTTTGATAATTAATATAA+TGG - chr6.4:48421045-48421064 MS.gene72621:intron 10.0%
!!! ATTTTTCATGAAATAATATT+TGG - chr6.4:48421676-48421695 MS.gene72621:intron 10.0%
!!! TTTTAAAGTTTTGATTTTTA+AGG - chr6.4:48421191-48421210 MS.gene72621:intron 10.0%
!! TTACAAATGAAAGAAATATT+AGG + chr6.4:48422182-48422201 None:intergenic 15.0%
!!! AACAATTTTTGAAATTTTGA+AGG - chr6.4:48421233-48421252 MS.gene72621:intron 15.0%
!!! ATTTTTAAGGTTTTATCAAT+TGG - chr6.4:48421080-48421099 MS.gene72621:intron 15.0%
!!! GACAATTTATGAAATTTTTA+AGG - chr6.4:48421067-48421086 MS.gene72621:intron 15.0%
!!! GGAATTTTTTGAAATTTTTA+AGG - chr6.4:48421149-48421168 MS.gene72621:intron 15.0%
!!! TAAGGTTTTTTTTTTCAATT+GGG - chr6.4:48421167-48421186 MS.gene72621:intron 15.0%
!!! TGTAATAACTTTTTTAGATT+TGG - chr6.4:48421647-48421666 MS.gene72621:intron 15.0%
!!! TTAAGGTTTTTTTTTTCAAT+TGG - chr6.4:48421166-48421185 MS.gene72621:intron 15.0%
!!! TTTTTAAGGTTTTATCAATT+GGG - chr6.4:48421081-48421100 MS.gene72621:intron 15.0%
!! ATGATTCAACAATGAATAAA+AGG + chr6.4:48421302-48421321 None:intergenic 20.0%
!! TCTTAATTAAGTAGCATTAT+TGG - chr6.4:48422129-48422148 MS.gene72621:intron 20.0%
!! TGATTCAACAATGAATAAAA+GGG + chr6.4:48421301-48421320 None:intergenic 20.0%
!! TTTATAAATGACACTTGATT+AGG + chr6.4:48422063-48422082 None:intergenic 20.0%
!! TTTATTCATTGTTGAATCAT+TGG - chr6.4:48421302-48421321 MS.gene72621:intron 20.0%
!!! AAGGTTTTTTTTTTCAATTG+GGG - chr6.4:48421168-48421187 MS.gene72621:intron 20.0%
!!! AAGTTTTGATTTTTAAGGTT+TGG - chr6.4:48421196-48421215 MS.gene72621:intron 20.0%
!!! ACTTTGTTTTATGTTGTTTT+TGG - chr6.4:48421418-48421437 MS.gene72621:CDS 20.0%
!!! TTTGAAGGTTTTTCTTAATT+GGG - chr6.4:48421248-48421267 MS.gene72621:intron 20.0%
!!! TTTTAAGGTTTTATCAATTG+GGG - chr6.4:48421082-48421101 MS.gene72621:intron 20.0%
!!! TTTTGAAGGTTTTTCTTAAT+TGG - chr6.4:48421247-48421266 MS.gene72621:intron 20.0%
! AATTGGGGTTTCTTAAAATT+TGG - chr6.4:48421097-48421116 MS.gene72621:intron 25.0%
! AGATGAATTGACATTTGAAT+AGG + chr6.4:48422095-48422114 None:intergenic 25.0%
! ATTGGGGTTTCTTAAAATTT+GGG - chr6.4:48421098-48421117 MS.gene72621:intron 25.0%
! CCTTATAGATACAAAAAGAA+TGG - chr6.4:48421458-48421477 MS.gene72621:intron 25.0%
! GAACATAAGAAGAAGAAAAT+GGG - chr6.4:48421849-48421868 MS.gene72621:intron 25.0%
! TGAACATAAGAAGAAGAAAA+TGG - chr6.4:48421848-48421867 MS.gene72621:intron 25.0%
!! CCATTCTTTTTGTATCTATA+AGG + chr6.4:48421461-48421480 None:intergenic 25.0%
!! GATCTTGTGATTTTTCTTTT+GGG - chr6.4:48421000-48421019 MS.gene72621:intron 25.0%
!! TGATCTTGTGATTTTTCTTT+TGG - chr6.4:48420999-48421018 MS.gene72621:intron 25.0%
!! TGTTTGGAATTGAATATGAT+GGG - chr6.4:48421128-48421147 MS.gene72621:intron 25.0%
!!! AAAAAAGTATTATGTCTGAG+CGG + chr6.4:48421991-48422010 None:intergenic 25.0%
!!! AAATTTGGGTTTTTAGTGTT+TGG - chr6.4:48421112-48421131 MS.gene72621:intron 25.0%
!!! CTTTTGGGTTTCTTAAAAAT+TGG - chr6.4:48421015-48421034 MS.gene72621:intron 25.0%
AAAGAATGGAACTCAAGAAA+AGG - chr6.4:48421472-48421491 MS.gene72621:intron 30.0%
AACTCAAGAAAAGGAAATAG+AGG - chr6.4:48421481-48421500 MS.gene72621:intron 30.0%
CTTCTTCTTATGTTCATTTC+TGG + chr6.4:48421844-48421863 None:intergenic 30.0%
TTCATTGTTGAATCATTGGA+AGG - chr6.4:48421306-48421325 MS.gene72621:intron 30.0%
! CTTTTGATAAGTGCTCAAAA+GGG - chr6.4:48421506-48421525 MS.gene72621:intron 30.0%
! GTGTTTGGAATTGAATATGA+TGG - chr6.4:48421127-48421146 MS.gene72621:intron 30.0%
! GTTTTCTTGCAGAGTAATTT+GGG - chr6.4:48421730-48421749 MS.gene72621:CDS 30.0%
! TCTTTTGATAAGTGCTCAAA+AGG - chr6.4:48421505-48421524 MS.gene72621:intron 30.0%
! TGTTTTCTTGCAGAGTAATT+TGG - chr6.4:48421729-48421748 MS.gene72621:CDS 30.0%
AAGAAATATGCCCATATGCT+TGG - chr6.4:48422229-48422248 MS.gene72621:CDS 35.0%
AATAACTCCAGCTTCTTCTA+TGG + chr6.4:48421603-48421622 None:intergenic 35.0%
ATAGAAGAAGCTGGAGTTAT+TGG - chr6.4:48421602-48421621 MS.gene72621:CDS 35.0%
ATGTATCCTTCATGCATAGT+GGG + chr6.4:48421784-48421803 None:intergenic 35.0%
CATTTGCAACGTTATGAACA+AGG - chr6.4:48420942-48420961 MS.gene72621:CDS 35.0%
TGCAGAGTAATTTGGGTAAA+TGG - chr6.4:48421737-48421756 MS.gene72621:CDS 35.0%
TTAGCAAGGAGTTGGATAAT+TGG - chr6.4:48421818-48421837 MS.gene72621:intron 35.0%
TTGGGAAAAAGATGAAACCA+TGG - chr6.4:48421556-48421575 MS.gene72621:intron 35.0%
!! GAAGGAAGCTTTAGATGAAT+TGG - chr6.4:48422254-48422273 MS.gene72621:CDS 35.0%
AACTCCTTGCTAACAAGCAA+AGG + chr6.4:48421811-48421830 None:intergenic 40.0%
ACTCCTTGCTAACAAGCAAA+GGG + chr6.4:48421810-48421829 None:intergenic 40.0%
AGAGAAACCATAGAAGAAGC+TGG - chr6.4:48421593-48421612 MS.gene72621:CDS 40.0%
AGTGGAATGCAGTTTCCAAA+AGG - chr6.4:48421530-48421549 MS.gene72621:intron 40.0%
ATAAGTGCTCAAAAGGGTAG+TGG - chr6.4:48421512-48421531 MS.gene72621:intron 40.0%
ATGACACTTGATTAGGCTCA+AGG + chr6.4:48422056-48422075 None:intergenic 40.0%
CATGTATCCTTCATGCATAG+TGG + chr6.4:48421785-48421804 None:intergenic 40.0%
CTTCCTTCATCCAAGCATAT+GGG + chr6.4:48422242-48422261 None:intergenic 40.0%
TGCAAATGTCTACCAGTTCT+AGG + chr6.4:48420930-48420949 None:intergenic 40.0%
TGTAATCATGCAGTTGACTG+TGG - chr6.4:48422197-48422216 MS.gene72621:intron 40.0%
! TCTTTTTCCCAACCTCCTTT+TGG + chr6.4:48421548-48421567 None:intergenic 40.0%
! TGTTTGGTTTCTCCTAGAAC+TGG - chr6.4:48420915-48420934 MS.gene72621:CDS 40.0%
! TTTGCTTGTTAGCAAGGAGT+TGG - chr6.4:48421810-48421829 MS.gene72621:intron 40.0%
ATGCCCATATGCTTGGATGA+AGG - chr6.4:48422236-48422255 MS.gene72621:CDS 45.0%
GCAGTTTCCAAAAGGAGGTT+GGG - chr6.4:48421538-48421557 MS.gene72621:intron 45.0%
GCTTCCTTCATCCAAGCATA+TGG + chr6.4:48422243-48422262 None:intergenic 45.0%
GGAATGCAGTTTCCAAAAGG+AGG - chr6.4:48421533-48421552 MS.gene72621:intron 45.0%
TGCAGTTTCCAAAAGGAGGT+TGG - chr6.4:48421537-48421556 MS.gene72621:intron 45.0%
! AAGGATGTCGTCAAGTTGTG+GGG - chr6.4:48420961-48420980 MS.gene72621:CDS 45.0%
! ACAAGGATGTCGTCAAGTTG+TGG - chr6.4:48420959-48420978 MS.gene72621:CDS 45.0%
! CAAGGATGTCGTCAAGTTGT+GGG - chr6.4:48420960-48420979 MS.gene72621:CDS 45.0%
! GTTCCCTTTGCTTGTTAGCA+AGG - chr6.4:48421804-48421823 MS.gene72621:intron 45.0%
! TCTCAAAGCAGCTTGTTCCA+TGG + chr6.4:48421576-48421595 None:intergenic 45.0%
!! ATCCTTCATGCATAGTGGGT+TGG + chr6.4:48421780-48421799 None:intergenic 45.0%
TTATGTCGCAGCGCAGATTG+AGG - chr6.4:48422009-48422028 MS.gene72621:intron 50.0%
CGCCAACCCACTATGCATGA+AGG - chr6.4:48421775-48421794 MS.gene72621:intron 55.0%
TGTCTGAGCGGCTACGATTG+AGG + chr6.4:48421979-48421998 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 48420909 48422302 48420909 ID=MS.gene72621
chr6.4 mRNA 48420909 48422302 48420909 ID=MS.gene72621.t1;Parent=MS.gene72621
chr6.4 exon 48422229 48422302 48422229 ID=MS.gene72621.t1.exon1;Parent=MS.gene72621.t1
chr6.4 CDS 48422229 48422302 48422229 ID=cds.MS.gene72621.t1;Parent=MS.gene72621.t1
chr6.4 exon 48421681 48421760 48421681 ID=MS.gene72621.t1.exon2;Parent=MS.gene72621.t1
chr6.4 CDS 48421681 48421760 48421681 ID=cds.MS.gene72621.t1;Parent=MS.gene72621.t1
chr6.4 exon 48421577 48421644 48421577 ID=MS.gene72621.t1.exon3;Parent=MS.gene72621.t1
chr6.4 CDS 48421577 48421644 48421577 ID=cds.MS.gene72621.t1;Parent=MS.gene72621.t1
chr6.4 exon 48421341 48421469 48421341 ID=MS.gene72621.t1.exon4;Parent=MS.gene72621.t1
chr6.4 CDS 48421341 48421469 48421341 ID=cds.MS.gene72621.t1;Parent=MS.gene72621.t1
chr6.4 exon 48420909 48421001 48420909 ID=MS.gene72621.t1.exon5;Parent=MS.gene72621.t1
chr6.4 CDS 48420909 48421001 48420909 ID=cds.MS.gene72621.t1;Parent=MS.gene72621.t1
Gene Sequence

>MS.gene72621

ATGATGTGTTTGGTTTCTCCTAGAACTGGTAGACATTTGCAACGTTATGAACAAGGATGTCGTCAAGTTGTGGGGTGTGTTTCTCTCTCTTGATCTTGTGATTTTTCTTTTGGGTTTCTTAAAAATTGGATTTTTAATGTTTGATAATTAATATAATGGACAATTTATGAAATTTTTAAGGTTTTATCAATTGGGGTTTCTTAAAATTTGGGTTTTTAGTGTTTGGAATTGAATATGATGGGAATTTTTTGAAATTTTTAAGGTTTTTTTTTTCAATTGGGGTTTTAAAGTTTTGATTTTTAAGGTTTGGAATTGAAGATGATGAACAATTTTTGAAATTTTGAAGGTTTTTCTTAATTGGGTTTTTTTCTAAATGATTTTAAAGTATACCCTTTTATTCATTGTTGAATCATTGGAAGGAGAAGTAAAAAGTCAACTTTTTTTTTTTTTTTTGTGAATTTAATTTGAATTTGTTGTTTTGATGTATTTGTGTTTGATTTGTTAATCTAACTTTGTTTTATGTTGTTTTTGGAATTGAACAGATGCATACCTTATAGATACAAAAAGAATGGAACTCAAGAAAAGGAAATAGAGGTTCTTTTGATAAGTGCTCAAAAGGGTAGTGGAATGCAGTTTCCAAAAGGAGGTTGGGAAAAAGATGAAACCATGGAACAAGCTGCTTTGAGAGAAACCATAGAAGAAGCTGGAGTTATTGGAAGTGTTGAAGTAAGTATTTATTGTAATAACTTTTTTAGATTTGGTTTTTCATTTTTCATGAAATAATATTTGGTACTTTGATTCTAACTTTGTGTATTATGATTGTTTTCTTGCAGAGTAATTTGGGTAAATGGTACTATAAGAGCAAACGCCAACCCACTATGCATGAAGGATACATGTTCCCTTTGCTTGTTAGCAAGGAGTTGGATAATTGGCCAGAAATGAACATAAGAAGAAGAAAATGGGTAAGTGTATATGAGACCTATATTCAACTTTTATAATATTCTAATCATGTTTGCGACACGATTCTTGCTATTGCAGAGAATTGCAGTCAAATACAACTTTTGCAACCTCAATCGTAGCCGCTCAGACATAATACTTTTTTATGTCGCAGCGCAGATTGAGGTTGCATACATGATTTTCAAAACCTTGAGCCTAATCAAGTGTCATTTATAAATTATTGTGTCCTATTCAAATGTCAATTCATCTTGCAAATCGCAATATCTTAATTAAGTAGCATTATTGGTTTACTATTATTATTTTTTGTTAGTGTCCTAATATTTCTTTCATTTGTAATCATGCAGTTGACTGTGGATGAAGCAAAAGAAATATGCCCATATGCTTGGATGAAGGAAGCTTTAGATGAATTGGTTAGTAGACAAACCAATTCAATCTAA

Protein sequence

>MS.gene72621.t1

MMCLVSPRTGRHLQRYEQGCRQVVGCIPYRYKKNGTQEKEIEVLLISAQKGNETMEQAALRETIEEAGVIGSVESNLGKWYYKSKRQPTMHEGYMFPLLVSKELDNWPEMNIRRRKWLTVDEAKEICPYAWMKEALDELVSRQTNSI