AlfalfaGEDB | MS.gene72811

M. sativa cultivar XinJiangDaYe / MS.gene72811

KEGG: K10899
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72811.t1	RHN54641.1	98	98	2	0	1	98	546	643	5.30E-43	183.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72811.t1	Q9FT73	66.3	98	29	1	1	98	546	639	5.4e-28	124.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72811.t1	G7K6R5	98.0	98	2	0	1	98	529	626	3.8e-43	183.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene72811	0.831589	1.53E-55	-1.69E-46
MS.gene049435	MS.gene72811	0.805159	1.53E-49	-1.69E-46
MS.gene049501	MS.gene72811	0.827881	1.22E-54	-1.69E-46
MS.gene049850	MS.gene72811	0.830711	2.52E-55	-1.69E-46
MS.gene050438	MS.gene72811	0.822725	2.03E-53	-1.69E-46
MS.gene050442	MS.gene72811	0.821336	4.26E-53	-1.69E-46
MS.gene050815	MS.gene72811	0.825278	5.11E-54	-1.69E-46
MS.gene050844	MS.gene72811	0.805401	1.36E-49	-1.69E-46
MS.gene050959	MS.gene72811	0.805003	1.65E-49	-1.69E-46
MS.gene051180	MS.gene72811	0.802478	5.51E-49	-1.69E-46
MS.gene051247	MS.gene72811	0.819269	1.27E-52	-1.69E-46
MS.gene051265	MS.gene72811	0.818416	1.98E-52	-1.69E-46
MS.gene051344	MS.gene72811	0.810684	1.02E-50	-1.69E-46
MS.gene051388	MS.gene72811	0.801827	7.49E-49	-1.69E-46
MS.gene052020	MS.gene72811	0.849259	3.63E-60	-1.69E-46
MS.gene052519	MS.gene72811	0.818293	2.11E-52	-1.69E-46
MS.gene052611	MS.gene72811	0.820068	8.34E-53	-1.69E-46
MS.gene052613	MS.gene72811	0.816969	4.20E-52	-1.69E-46
MS.gene052773	MS.gene72811	0.803078	4.14E-49	-1.69E-46
MS.gene052863	MS.gene72811	0.839905	1.20E-57	-1.69E-46
MS.gene052896	MS.gene72811	0.820046	8.44E-53	-1.69E-46
MS.gene053008	MS.gene72811	0.81992	9.01E-53	-1.69E-46
MS.gene053221	MS.gene72811	0.804756	1.85E-49	-1.69E-46
MS.gene053226	MS.gene72811	0.815512	8.90E-52	-1.69E-46
MS.gene054236	MS.gene72811	0.806262	8.96E-50	-1.69E-46
MS.gene054472	MS.gene72811	0.817924	2.56E-52	-1.69E-46
MS.gene055357	MS.gene72811	0.803887	2.81E-49	-1.69E-46
MS.gene055466	MS.gene72811	0.804945	1.69E-49	-1.69E-46
MS.gene055512	MS.gene72811	0.801984	6.96E-49	-1.69E-46
MS.gene055608	MS.gene72811	0.809097	2.24E-50	-1.69E-46
MS.gene055818	MS.gene72811	0.817268	3.60E-52	-1.69E-46
MS.gene056173	MS.gene72811	0.802035	6.79E-49	-1.69E-46
MS.gene057915	MS.gene72811	0.807452	5.02E-50	-1.69E-46
MS.gene058168	MS.gene72811	0.806628	7.50E-50	-1.69E-46
MS.gene058636	MS.gene72811	0.809769	1.61E-50	-1.69E-46
MS.gene05954	MS.gene72811	0.805368	1.38E-49	-1.69E-46
MS.gene059842	MS.gene72811	0.805162	1.52E-49	-1.69E-46
MS.gene059937	MS.gene72811	0.813841	2.09E-51	-1.69E-46
MS.gene060322	MS.gene72811	0.815181	1.05E-51	-1.69E-46
MS.gene060429	MS.gene72811	0.804291	2.32E-49	-1.69E-46
MS.gene060803	MS.gene72811	0.833766	4.42E-56	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72811.t1	MTR_5g026590	97.959	98	2	0	1	98	529	626	3.03e-59	192

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene72811.t1	AT1G31360	66.327	98	29	1	1	98	421	514	1.35e-36	130
MS.gene72811.t1	AT1G31360	66.327	98	29	1	1	98	546	639	1.42e-36	130
MS.gene72811.t1	AT1G31360	66.327	98	29	1	1	98	546	639	1.72e-36	130
MS.gene72811.t1	AT1G31360	66.327	98	29	1	1	98	580	673	1.77e-36	130

Find 18 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTGCCTCTTCCAGTGAGTT+TGG	0.267298	5.1:+17216985	None:intergenic
AAACGCAGCCTCGGGTCATC+TGG	0.375770	5.1:-17217120	MS.gene72811:CDS
CAGCCTCGGGTCATCTGGCT+TGG	0.413431	5.1:-17217115	MS.gene72811:CDS
TGCAAAATTTGCTTCGCCAA+TGG	0.440678	5.1:+17217499	None:intergenic
AAAGAACTTTCTTCCACTCA+TGG	0.477688	5.1:-17217069	MS.gene72811:CDS
AAAACCAAACTCACTGGAAG+AGG	0.507726	5.1:-17216989	None:intergenic
TTACATTACTGTGGGGCCAT+TGG	0.508759	5.1:-17217515	MS.gene72811:CDS
TAAGATTGAAACGCAGCCTC+GGG	0.520222	5.1:-17217128	MS.gene72811:CDS
ACAAATGCTTACATTACTGT+GGG	0.528063	5.1:-17217523	MS.gene72811:CDS
GTAAGATTGAAACGCAGCCT+CGG	0.535718	5.1:-17217129	MS.gene72811:CDS
TACAAATGCTTACATTACTG+TGG	0.538641	5.1:-17217524	MS.gene72811:CDS
TGAGTAGACAAGACAGAGTG+TGG	0.573006	5.1:+17217036	None:intergenic
TGGGAGTATTCCTCCATGAG+TGG	0.623965	5.1:+17217056	None:intergenic
GAAGCTTGAGATTTCTACAG+AGG	0.633669	5.1:-17217169	MS.gene72811:CDS
GAACTTTCTTCCACTCATGG+AGG	0.642311	5.1:-17217066	MS.gene72811:CDS
GAGTAGACAAGACAGAGTGT+GGG	0.645165	5.1:+17217037	None:intergenic
GCTCCAAGCCAGATGACCCG+AGG	0.687823	5.1:+17217112	None:intergenic
CAAATGCTTACATTACTGTG+GGG	0.745077	5.1:-17217522	MS.gene72811:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTCTTATTTAAAGTATTTCA+TGG	+	chr5.1:17217182-17217201	None:intergenic	15.0%
!!	TCCATTAAGCTAATTTATTA+TGG	-	chr5.1:17217098-17217117	MS.gene72811:CDS	20.0%
!	ACCAAAAAAAAAATCCAAAC+AGG	+	chr5.1:17217337-17217356	None:intergenic	25.0%
!	TTGAATGAAAAAACATGACT+CGG	+	chr5.1:17217159-17217178	None:intergenic	25.0%
!!	GCCATAATAAATTAGCTTAA+TGG	+	chr5.1:17217102-17217121	None:intergenic	25.0%
!!!	TGGATTTTTTTTTTGGTGTA+GGG	-	chr5.1:17217340-17217359	MS.gene72811:intron	25.0%
!!!	TTGGATTTTTTTTTTGGTGT+AGG	-	chr5.1:17217339-17217358	MS.gene72811:intron	25.0%
	ACAAATGCTTACATTACTGT+GGG	-	chr5.1:17217020-17217039	MS.gene72811:CDS	30.0%
	AGAAACTAGCTCAATGAATT+AGG	-	chr5.1:17217198-17217217	MS.gene72811:intron	30.0%
	TACAAATGCTTACATTACTG+TGG	-	chr5.1:17217019-17217038	MS.gene72811:CDS	30.0%
!!	TTTTTCCGACTCTAATTTGT+CGG	-	chr5.1:17217225-17217244	MS.gene72811:intron	30.0%
!!!	GCCTGTTTGGATTTTTTTTT+TGG	-	chr5.1:17217333-17217352	MS.gene72811:intron	30.0%
	AAAATTTGCAAACTGAGTGC+AGG	+	chr5.1:17217280-17217299	None:intergenic	35.0%
	AAAGAACTTTCTTCCACTCA+TGG	-	chr5.1:17217474-17217493	MS.gene72811:intron	35.0%
	AAGAACCGACAAATTAGAGT+CGG	+	chr5.1:17217233-17217252	None:intergenic	35.0%
	CAAATGCTTACATTACTGTG+GGG	-	chr5.1:17217021-17217040	MS.gene72811:CDS	35.0%
	TTCTCAAAAACCAAACTCAC+TGG	-	chr5.1:17217548-17217567	MS.gene72811:CDS	35.0%
	GAAGCTTGAGATTTCTACAG+AGG	-	chr5.1:17217374-17217393	MS.gene72811:intron	40.0%
!	TGCAAAATTTGCTTCGCCAA+TGG	+	chr5.1:17217047-17217066	None:intergenic	40.0%
!	TTGGCGAAGCAAATTTTGCA+AGG	-	chr5.1:17217047-17217066	MS.gene72811:CDS	40.0%
	GAACTTTCTTCCACTCATGG+AGG	-	chr5.1:17217477-17217496	MS.gene72811:intron	45.0%
	GAGTAGACAAGACAGAGTGT+GGG	+	chr5.1:17217509-17217528	None:intergenic	45.0%
	GTAAGATTGAAACGCAGCCT+CGG	-	chr5.1:17217414-17217433	MS.gene72811:intron	45.0%
	TAAGATTGAAACGCAGCCTC+GGG	-	chr5.1:17217415-17217434	MS.gene72811:intron	45.0%
	TGAGTAGACAAGACAGAGTG+TGG	+	chr5.1:17217510-17217529	None:intergenic	45.0%
	TTACATTACTGTGGGGCCAT+TGG	-	chr5.1:17217028-17217047	MS.gene72811:CDS	45.0%
!!	CTTTCTCTGAGATGCTAGTG+TGG	-	chr5.1:17217250-17217269	MS.gene72811:intron	45.0%
	ACCAACTGCTTGTGCCTGTT+TGG	-	chr5.1:17217320-17217339	MS.gene72811:intron	50.0%
	TCCAAACAGGCACAAGCAGT+TGG	+	chr5.1:17217324-17217343	None:intergenic	50.0%
!!	TGGGAGTATTCCTCCATGAG+TGG	+	chr5.1:17217490-17217509	None:intergenic	50.0%
	AAACGCAGCCTCGGGTCATC+TGG	-	chr5.1:17217423-17217442	MS.gene72811:intron	60.0%
	CAGCCTCGGGTCATCTGGCT+TGG	-	chr5.1:17217428-17217447	MS.gene72811:intron	65.0%
	GCTCCAAGCCAGATGACCCG+AGG	+	chr5.1:17217434-17217453	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	17216990	17217575	17216990	ID=MS.gene72811
chr5.1	mRNA	17216990	17217575	17216990	ID=MS.gene72811.t1;Parent=MS.gene72811
chr5.1	exon	17217497	17217575	17217497	ID=MS.gene72811.t1.exon1;Parent=MS.gene72811.t1
chr5.1	CDS	17217497	17217575	17217497	ID=cds.MS.gene72811.t1;Parent=MS.gene72811.t1
chr5.1	exon	17216990	17217204	17216990	ID=MS.gene72811.t1.exon2;Parent=MS.gene72811.t1
chr5.1	CDS	17216990	17217204	17216990	ID=cds.MS.gene72811.t1;Parent=MS.gene72811.t1

Gene Sequence

>MS.gene72811

AAAGAAGAATTTCAGCATACAGCTTATTCTACAAATGCTTACATTACTGTGGGGCCATTGGCGAAGCAAATTTTGCAAGGTACACACGTTTCTCATTATAGATGTCAATCCATTAAGCTAATTTATTATGGCAAAATCTATTGAGTTTTTGTGTTTTACATCTGTACCGAGTCATGTTTTTTCATTCAACCATGAAATACTTTAAATAAGAAACTAGCTCAATGAATTAGGCTAATTTTTCCGACTCTAATTTGTCGGTTCTTTCTCTGAGATGCTAGTGTGGTACACCTGCACTCAGTTTGCAAATTTTCATGTACACGTATGTTGATTACCAACTGCTTGTGCCTGTTTGGATTTTTTTTTTGGTGTAGGGAAGAAGACTGTGAAGCTTGAGATTTCTACAGAGGAGAAAACGAAAGCTTCTGTAAGATTGAAACGCAGCCTCGGGTCATCTGGCTTGGAGCTAAAACTTGATGAGCTGAGAAAAGAACTTTCTTCCACTCATGGAGGAATACTCCCACACTCTGTCTTGTCTACTCAACAAATCAGCTTACTATGTTCTCAAAAACCAAACTCACTGGAAGAG

Protein sequence

>MS.gene72811.t1

KEEFQHTAYSTNAYITVGPLAKQILQGKKTVKLEISTEEKTKASVRLKRSLGSSGLELKLDELRKELSSTHGGILPHSVLSTQQISLLCSQKPNSLEE