AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75068

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75068.t1 MTR_6g091800 94.286 315 14 1 1 311 1 315 0.0 602
MS.gene75068.t1 MTR_6g091800 87.611 339 14 2 1 311 1 339 0.0 590
MS.gene75068.t1 MTR_6g091800 86.538 312 14 2 1 284 1 312 0.0 535
MS.gene75068.t1 MTR_6g034145 95.019 261 13 0 51 311 1 261 0.0 511
MS.gene75068.t1 MTR_6g091800 81.270 315 14 2 1 311 1 274 0.0 499
MS.gene75068.t1 MTR_6g091800 75.516 339 14 3 1 311 1 298 1.26e-175 488
MS.gene75068.t1 MTR_6g092210 89.272 261 22 1 51 311 1 255 6.04e-171 474
MS.gene75068.t1 MTR_6g091800 92.713 247 14 1 1 243 1 247 1.24e-166 464
MS.gene75068.t1 MTR_6g091800 27.751 209 122 7 110 305 18 210 1.64e-12 67.0
MS.gene75068.t1 MTR_6g091800 84.502 271 14 2 1 243 1 271 1.43e-161 452
MS.gene75068.t1 MTR_6g091910 88.614 202 21 1 110 311 18 217 1.50e-128 365
MS.gene75068.t1 MTR_6g091910 36.364 220 113 4 1 205 1 208 1.38e-29 112
MS.gene75068.t1 MTR_3g104600 54.114 316 134 4 5 309 21 336 1.12e-119 347
MS.gene75068.t1 MTR_6g091800 90.761 184 13 1 1 180 1 184 1.66e-117 336
MS.gene75068.t1 MTR_6g091800 80.288 208 13 2 1 180 1 208 2.38e-112 324
MS.gene75068.t1 MTR_5g009500 36.102 313 163 7 20 307 43 343 5.20e-54 180
MS.gene75068.t1 MTR_8g027975 33.438 317 168 7 20 307 56 358 6.01e-50 169
MS.gene75068.t1 MTR_7g064980 33.882 304 161 7 20 302 30 314 3.00e-45 156
MS.gene75068.t1 MTR_3g098460 30.556 288 178 5 20 302 210 480 3.79e-42 152
MS.gene75068.t1 MTR_7g084400 31.864 295 168 6 19 302 213 485 3.89e-40 146
MS.gene75068.t1 MTR_8g096050 31.250 288 176 6 20 302 232 502 8.23e-40 146
MS.gene75068.t1 MTR_8g096050 30.890 191 119 4 20 208 326 505 4.74e-21 93.6
MS.gene75068.t1 MTR_1g012030 31.359 287 173 6 20 302 144 410 8.75e-40 144
MS.gene75068.t1 MTR_1g012030 29.082 196 121 5 12 203 227 408 5.75e-19 87.0
MS.gene75068.t1 MTR_1g012030 25.943 212 129 6 105 310 132 321 1.00e-11 65.5
MS.gene75068.t1 MTR_3g107510 29.965 287 177 5 20 302 127 393 5.76e-37 136
MS.gene75068.t1 MTR_3g107510 25.325 154 104 3 162 310 157 304 1.48e-11 65.1
MS.gene75068.t1 MTR_5g081150 33.113 302 172 6 18 302 33 321 1.33e-35 131
MS.gene75068.t1 MTR_4g114320 28.276 290 178 6 20 302 128 394 2.14e-34 129
MS.gene75068.t1 MTR_4g114320 28.500 200 126 4 12 208 212 397 5.27e-17 81.3
MS.gene75068.t1 MTR_7g068340 29.123 285 162 5 26 299 98 353 1.86e-33 126
MS.gene75068.t1 MTR_4g119770 27.329 322 182 6 20 303 19 326 3.54e-33 125
MS.gene75068.t1 MTR_4g119770 25.980 204 134 5 109 301 12 209 1.90e-15 76.3
MS.gene75068.t1 MTR_3g104500 28.296 311 185 9 15 304 30 323 6.22e-33 124
MS.gene75068.t1 MTR_3g104500 24.638 207 144 5 110 310 33 233 8.38e-14 71.2
MS.gene75068.t1 MTR_2g436930 26.415 318 186 6 20 302 20 324 3.88e-32 122
MS.gene75068.t1 MTR_2g436930 28.019 207 134 5 104 301 8 208 1.71e-18 84.7
MS.gene75068.t1 MTR_4g016730 28.621 290 154 6 26 299 100 352 7.79e-29 114
MS.gene75068.t1 MTR_8g090210 30.063 316 173 7 17 306 108 401 9.72e-29 114
MS.gene75068.t1 MTR_4g078545 29.097 299 187 7 15 299 71 358 8.25e-27 108
MS.gene75068.t1 MTR_2g098070 28.094 299 190 7 15 299 62 349 1.79e-26 107
MS.gene75068.t1 MTR_4g119770 28.405 257 139 5 20 245 19 261 4.62e-26 104
MS.gene75068.t1 MTR_4g119770 25.980 204 134 5 109 301 12 209 8.75e-16 76.3
MS.gene75068.t1 MTR_3g074490 29.936 314 172 8 17 304 114 405 6.37e-26 106
MS.gene75068.t1 MTR_4g005330 25.243 309 183 9 20 304 5 289 2.12e-25 103
MS.gene75068.t1 MTR_4g005330 25.159 314 187 9 15 304 66 355 4.08e-25 103
MS.gene75068.t1 MTR_3g028590 28.094 299 190 7 15 299 89 376 8.08e-25 103
MS.gene75068.t1 MTR_3g028590 28.094 299 190 7 15 299 93 380 8.11e-25 103
MS.gene75068.t1 MTR_7g109135 27.645 293 187 6 21 302 28 306 1.35e-24 101
MS.gene75068.t1 MTR_7g109135 29.208 202 130 6 113 311 24 215 1.05e-15 76.6
MS.gene75068.t1 MTR_8g107270 27.946 297 186 11 25 309 28 308 3.30e-24 100
MS.gene75068.t1 MTR_8g036880 27.759 299 192 7 15 299 98 386 9.23e-24 100
MS.gene75068.t1 MTR_2g436930 28.972 214 121 3 107 302 34 234 3.45e-23 96.3
MS.gene75068.t1 MTR_2g436930 23.009 226 154 5 2 214 20 238 8.48e-11 61.6
MS.gene75068.t1 MTR_5g081150 33.624 229 122 6 18 229 33 248 1.01e-22 95.1
MS.gene75068.t1 MTR_5g081150 27.632 228 140 7 93 309 16 229 5.55e-12 65.1
MS.gene75068.t1 MTR_5g081150 33.478 230 123 6 17 229 32 248 1.02e-22 95.1
MS.gene75068.t1 MTR_5g081150 27.632 228 140 7 93 309 16 229 5.57e-12 65.1
MS.gene75068.t1 MTR_1g087840 28.025 314 190 10 10 302 5 303 1.31e-22 95.9
MS.gene75068.t1 MTR_1g087840 28.125 192 123 7 124 311 32 212 1.72e-11 64.3
MS.gene75068.t1 MTR_7g109135 26.962 293 182 6 21 302 28 299 3.77e-22 94.7
MS.gene75068.t1 MTR_7g109135 27.228 202 127 6 113 311 24 208 3.94e-11 63.2
MS.gene75068.t1 MTR_5g055410 26.667 285 188 6 27 302 33 305 3.93e-21 92.4
MS.gene75068.t1 MTR_7g084400 31.532 222 118 7 19 225 207 409 4.35e-21 93.2
MS.gene75068.t1 MTR_7g084400 26.020 196 120 5 111 300 207 383 7.76e-14 72.0
MS.gene75068.t1 MTR_7g109135 26.556 241 157 5 68 302 2 228 5.16e-18 82.0
MS.gene75068.t1 MTR_5g030530 25.828 302 199 10 16 302 5 296 1.94e-17 81.3
MS.gene75068.t1 MTR_8g076760 25.283 265 172 6 58 309 61 312 4.38e-17 80.5
MS.gene75068.t1 MTR_8g098240 24.468 282 191 6 20 300 325 585 1.89e-16 80.1
MS.gene75068.t1 MTR_7g109135 28.994 169 110 4 140 302 43 207 2.14e-15 74.3
MS.gene75068.t1 MTR_8g066975 25.000 300 204 9 16 302 5 296 2.91e-15 75.1
MS.gene75068.t1 MTR_2g102010 30.481 187 112 3 28 208 123 297 3.52e-14 72.0
MS.gene75068.t1 MTR_4g012850 26.736 288 171 11 9 277 1 267 8.94e-14 70.9
MS.gene75068.t1 MTR_4g012850 29.670 182 112 3 22 199 117 286 1.82e-13 70.1
MS.gene75068.t1 MTR_5g073040 25.763 295 185 9 20 303 44 315 2.21e-12 67.0
MS.gene75068.t1 MTR_7g117030 23.643 258 172 8 57 299 85 332 2.37e-12 67.0
MS.gene75068.t1 MTR_6g034515 25.806 155 110 1 57 211 198 347 8.56e-12 65.5
MS.gene75068.t1 MTR_6g034515 22.742 299 187 9 31 311 83 355 8.80e-11 62.4
MS.gene75068.t1 MTR_4g018750 21.519 316 217 8 1 303 1 298 1.04e-11 65.1
MS.gene75068.t1 MTR_4g018750 23.228 254 171 7 1 241 1 243 3.57e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75068.t1 AT4G26180 67.089 316 97 3 1 309 1 316 5.04e-153 432
MS.gene75068.t1 AT4G26180 66.323 291 91 3 1 284 1 291 4.30e-138 392
MS.gene75068.t1 AT1G14560 55.486 319 130 4 1 308 9 326 1.58e-118 344
MS.gene75068.t1 AT1G14560 54.915 295 121 4 1 284 9 302 1.69e-104 308
MS.gene75068.t1 AT1G14560 56.332 229 92 3 87 308 1 228 1.54e-86 259
MS.gene75068.t1 AT1G14560 26.904 197 121 4 21 202 31 219 5.60e-12 64.7
MS.gene75068.t1 AT4G01100 35.127 316 164 7 20 307 47 349 1.35e-53 179
MS.gene75068.t1 AT4G01100 34.615 312 163 7 24 307 1 299 1.04e-51 172
MS.gene75068.t1 AT4G01100 33.939 330 163 8 20 307 47 363 4.07e-51 173
MS.gene75068.t1 AT3G55640 34.853 307 166 8 16 303 35 326 1.92e-46 160
MS.gene75068.t1 AT3G55640 34.853 307 166 8 16 303 35 326 1.92e-46 160
MS.gene75068.t1 AT2G37890 35.410 305 163 7 17 302 43 332 2.19e-46 160
MS.gene75068.t1 AT3G53940 33.333 303 171 7 20 306 78 365 7.61e-45 156
MS.gene75068.t1 AT5G61810 33.446 296 163 8 16 302 62 332 4.64e-44 154
MS.gene75068.t1 AT5G61810 29.319 191 117 3 20 208 161 335 9.79e-17 80.1
MS.gene75068.t1 AT5G61810 33.446 296 163 8 16 302 205 475 1.08e-42 153
MS.gene75068.t1 AT5G61810 29.319 191 117 3 20 208 304 478 1.83e-16 80.1
MS.gene75068.t1 AT5G07320 31.206 282 166 6 25 302 219 476 1.83e-40 147
MS.gene75068.t1 AT5G07320 26.804 194 118 4 20 208 305 479 3.95e-14 73.2
MS.gene75068.t1 AT5G07320 25.828 151 108 2 154 303 236 383 9.26e-11 62.8
MS.gene75068.t1 AT5G51050 30.822 292 173 7 20 304 228 497 1.99e-39 145
MS.gene75068.t1 AT5G51050 27.604 192 120 4 20 208 323 498 7.99e-14 72.0
MS.gene75068.t1 AT4G32400 32.753 287 169 5 20 302 119 385 2.70e-39 142
MS.gene75068.t1 AT4G32400 27.670 206 129 7 12 212 202 392 1.71e-14 73.9
MS.gene75068.t1 AT4G32400 27.632 152 100 2 162 309 149 294 7.69e-12 65.9
MS.gene75068.t1 AT5G56450 30.990 313 174 8 15 302 30 325 2.23e-36 133
MS.gene75068.t1 AT3G51870 29.021 286 161 5 26 299 102 357 9.62e-36 132
MS.gene75068.t1 AT5G48970 27.103 321 183 6 20 302 27 334 5.09e-33 124
MS.gene75068.t1 AT5G48970 27.228 202 134 4 109 302 20 216 1.87e-17 82.0
MS.gene75068.t1 AT5G01500 29.110 292 159 6 26 302 130 388 4.76e-32 123
MS.gene75068.t1 AT3G21390 27.500 320 182 5 20 302 24 330 1.71e-31 120
MS.gene75068.t1 AT3G21390 27.451 204 135 4 105 301 13 210 2.12e-16 79.0
MS.gene75068.t1 AT3G21390 27.414 321 183 5 20 303 46 353 1.71e-31 121
MS.gene75068.t1 AT3G21390 27.451 204 135 4 105 301 35 232 2.93e-16 79.0
MS.gene75068.t1 AT3G55640 35.622 233 116 8 16 229 35 252 1.18e-30 116
MS.gene75068.t1 AT3G55640 28.780 205 121 6 110 303 37 227 3.53e-16 77.0
MS.gene75068.t1 AT3G55640 35.622 233 116 8 16 229 35 252 1.18e-30 116
MS.gene75068.t1 AT3G55640 28.780 205 121 6 110 303 37 227 3.53e-16 77.0
MS.gene75068.t1 AT3G53940 34.222 225 118 6 20 229 78 287 1.22e-30 117
MS.gene75068.t1 AT3G53940 28.502 207 123 6 106 301 68 260 7.98e-13 68.2
MS.gene75068.t1 AT5G48970 27.509 269 158 4 58 302 8 263 3.45e-29 112
MS.gene75068.t1 AT4G28390 29.808 312 188 8 8 299 66 366 7.36e-29 114
MS.gene75068.t1 AT4G28390 29.808 312 188 8 8 299 66 366 7.36e-29 114
MS.gene75068.t1 AT5G64970 30.380 316 172 7 17 306 133 426 4.93e-28 112
MS.gene75068.t1 AT5G64970 29.739 306 171 6 23 306 19 302 5.86e-28 110
MS.gene75068.t1 AT3G20240 25.902 305 182 8 20 302 57 339 9.26e-28 110
MS.gene75068.t1 AT5G13490 29.766 299 186 7 15 299 84 372 3.69e-27 109
MS.gene75068.t1 AT5G13490 29.766 299 186 7 15 299 84 372 3.69e-27 109
MS.gene75068.t1 AT3G08580 29.766 299 186 7 15 299 80 368 5.56e-26 106
MS.gene75068.t1 AT3G08580 29.766 299 186 7 15 299 80 368 5.56e-26 106
MS.gene75068.t1 AT5G17400 27.737 274 170 6 31 290 30 289 4.75e-25 102
MS.gene75068.t1 AT1G78180 29.560 318 168 9 17 304 126 417 6.03e-24 101
MS.gene75068.t1 AT1G25380 26.644 289 186 8 27 303 33 307 2.98e-23 98.6
MS.gene75068.t1 AT1G25380 26.644 289 186 8 27 303 33 307 2.98e-23 98.6
MS.gene75068.t1 AT2G47490 26.280 293 188 7 27 306 29 306 2.42e-20 89.7
MS.gene75068.t1 AT2G47490 28.646 192 121 6 124 311 30 209 2.61e-11 63.9
MS.gene75068.t1 AT5G64970 28.571 294 162 7 17 284 133 404 2.93e-20 90.9
MS.gene75068.t1 AT4G11440 25.850 294 182 7 20 302 335 603 2.40e-18 85.9
MS.gene75068.t1 AT4G11440 25.850 294 182 7 20 302 335 603 2.40e-18 85.9
MS.gene75068.t1 AT5G66380 27.237 257 164 7 58 303 57 301 8.84e-18 82.4
MS.gene75068.t1 AT1G14140 22.759 290 201 7 20 299 21 297 1.24e-14 73.6
MS.gene75068.t1 AT2G33820 24.585 301 194 12 15 296 14 300 1.73e-14 73.2
MS.gene75068.t1 AT5G46800 25.430 291 202 7 22 302 14 299 2.95e-14 72.4
MS.gene75068.t1 AT5G46800 25.430 291 202 7 22 302 14 299 2.95e-14 72.4
MS.gene75068.t1 AT5G46800 25.430 291 202 7 22 302 14 299 2.95e-14 72.4
MS.gene75068.t1 AT4G27940 23.723 274 182 7 46 302 144 407 1.74e-13 70.9
MS.gene75068.t1 AT2G30160 24.150 294 195 7 20 303 47 322 1.03e-12 68.2
MS.gene75068.t1 AT1G07030 22.680 291 200 4 20 303 45 317 1.43e-12 67.8
MS.gene75068.t1 AT2G39970 24.068 295 196 8 31 298 24 317 2.06e-12 67.4
MS.gene75068.t1 AT3G54110 21.725 313 220 7 1 303 1 298 4.74e-12 65.9
MS.gene75068.t1 AT3G54110 27.604 192 120 7 123 305 27 208 7.42e-11 62.4
MS.gene75068.t1 AT2G33820 23.948 309 194 13 15 296 14 308 5.17e-12 65.9
MS.gene75068.t1 AT1G79900 29.609 179 107 3 22 199 117 277 2.06e-11 63.9
MS.gene75068.t1 AT5G42130 25.163 306 186 11 24 311 127 407 4.30e-11 63.5
MS.gene75068.t1 AT5G58970 21.595 301 208 7 15 303 16 300 5.42e-11 62.8
MS.gene75068.t1 AT2G46320 24.231 260 168 9 55 299 107 352 7.12e-11 62.8
MS.gene75068.t1 AT1G74240 25.658 304 199 8 20 303 41 337 8.33e-11 62.8

Find 84 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACCAAGTTGAATGTTTC+TGG 0.120655 6.3:+83853282 MS.gene75068:CDS
TGGATCCGTGGCCGGTTTAT+TGG 0.168335 6.3:+83853621 MS.gene75068:CDS
ACGTCTCAAAATTCTCTTTC+AGG 0.214100 6.3:+83851170 MS.gene75068:CDS
TGCCAAGACCGAAGGTCTTT+TGG 0.240864 6.3:+83852841 MS.gene75068:CDS
CTTATGGCAGGGTCACTCTT+TGG 0.304103 6.3:+83853079 MS.gene75068:CDS
TCGGACTAAGTTAGCTTATC+AGG 0.313146 6.3:+83853141 MS.gene75068:CDS
GGTATGAAACTCGGCTCTTC+TGG 0.314043 6.3:-83852789 None:intergenic
GCCACAGCTCCGACACTCTT+TGG 0.326066 6.3:+83853499 MS.gene75068:intron
GTGAAAATTTGCAGGGGAAA+TGG 0.334185 6.3:+83852941 MS.gene75068:intron
GAGAGGGATGTGATCGAGTA+TGG 0.340180 6.3:-83851091 None:intergenic
TAGTCCACTTCATCTCTTGA+TGG 0.341198 6.3:-83854043 None:intergenic
AGGTCCTACCCTTGACCTTA+TGG 0.344397 6.3:+83853063 MS.gene75068:CDS
GGATCCGTGGCCGGTTTATT+GGG 0.353057 6.3:+83853622 MS.gene75068:CDS
TGGCATTAGAGGTCTGTATA+GGG 0.353243 6.3:+83853371 MS.gene75068:CDS
TGATACTCAGCCCTGAAAAC+AGG 0.379917 6.3:-83853855 None:intergenic
CATATCCTCTTGAAGTTGTC+AGG 0.390609 6.3:+83853656 MS.gene75068:CDS
GTGGCATTAGAGGTCTGTAT+AGG 0.393730 6.3:+83853370 MS.gene75068:CDS
TATAAAGAGGGTGGCATTAG+AGG 0.393834 6.3:+83853360 MS.gene75068:CDS
TTTGCTAAAGAACTTCTTGC+CGG 0.399304 6.3:+83851114 MS.gene75068:CDS
TTTCATACCACTGGACTGTC+TGG 0.418812 6.3:+83852803 MS.gene75068:CDS
TCCCAAAAGACCTTCGGTCT+TGG 0.420715 6.3:-83852843 None:intergenic
CAATCTTCGGTATTCCTCAT+AGG 0.426870 6.3:-83853011 None:intergenic
GTTGTTCCATCTGCGGCCAT+TGG 0.439759 6.3:+83853980 MS.gene75068:CDS
TCTCATAGTCCCTTTCAATT+CGG 0.446254 6.3:-83853794 None:intergenic
TTTGAAATTCTACTTCTATG+AGG 0.450212 6.3:+83853540 MS.gene75068:CDS
TAGAATTTCAAACCCGCGTA+TGG 0.453141 6.3:-83853529 None:intergenic
ATGGCAGGGTCACTCTTTGG+AGG 0.454307 6.3:+83853082 MS.gene75068:CDS
GCTGAGTATCAATTACATAA+AGG 0.458191 6.3:+83853867 MS.gene75068:CDS
CCAGAAACATTCAACTTGGT+TGG 0.463580 6.3:-83853282 None:intergenic
CAACCAAGTTGAATGTTTCT+GGG 0.469495 6.3:+83853283 MS.gene75068:CDS
CATCCCAGAAACATTCAACT+TGG 0.482679 6.3:-83853286 None:intergenic
TCTTCACTTTATGTCCTATG+AGG 0.493276 6.3:+83852997 MS.gene75068:CDS
AAGCTTGCACAATCAATCTT+CGG 0.494767 6.3:-83853024 None:intergenic
CCTACCCTTGACCTTATGGC+AGG 0.496875 6.3:+83853067 MS.gene75068:CDS
TGATTGCTCAAAAGCAAGGC+TGG 0.499574 6.3:+83853827 MS.gene75068:CDS
CAAAGAGTGACCCTGCCATA+AGG 0.502575 6.3:-83853078 None:intergenic
CTTATCCGCAGGACTTAACT+CGG 0.515435 6.3:+83853122 MS.gene75068:CDS
CTGAAGAAGCCGAATTGAAA+GGG 0.520218 6.3:+83853785 MS.gene75068:CDS
AGTACCATCAAGAGATGAAG+TGG 0.531658 6.3:+83854039 MS.gene75068:CDS
GAATGTCTGACCCAATAAAC+CGG 0.532252 6.3:-83853632 None:intergenic
ATAAAGTGAAGACCTGCAAA+AGG 0.532270 6.3:-83852986 None:intergenic
GTGAAGCCAATGGCCGCAGA+TGG 0.532601 6.3:-83853986 None:intergenic
CAAGTTGAATGTTTCTGGGA+TGG 0.532759 6.3:+83853287 MS.gene75068:CDS
CATCTCTTGATGGTACTCTC+AGG 0.533112 6.3:-83854033 None:intergenic
GTAGAATCCCAAAAGACCTT+CGG 0.538538 6.3:-83852849 None:intergenic
TCTGAAGAAGCCGAATTGAA+AGG 0.545458 6.3:+83853784 MS.gene75068:CDS
CGGCTTCTTCAGATGCCGCA+AGG 0.546244 6.3:-83853774 None:intergenic
AGAGGTCTGTATAGGGGAGT+AGG 0.546694 6.3:+83853378 MS.gene75068:CDS
CTGACATGTGGATCCGTGGC+CGG 0.555044 6.3:+83853613 MS.gene75068:CDS
AGAATTTCAAACCCGCGTAT+GGG 0.562448 6.3:-83853528 None:intergenic
AGCAGAATTGCCAAGACCGA+AGG 0.563234 6.3:+83852833 MS.gene75068:CDS
AGTTGTCAGGAGGCAAATGC+AGG 0.565986 6.3:+83853669 MS.gene75068:CDS
AGAAGTTCTTTAGCAAAGAG+AGG 0.570632 6.3:-83851108 None:intergenic
AATTCAGGTTGTTCCATCTG+CGG 0.571625 6.3:+83853973 MS.gene75068:intron
GAAGTTCTTTAGCAAAGAGA+GGG 0.572732 6.3:-83851107 None:intergenic
CAGGGGAAATGGAGCAAGTG+TGG 0.576065 6.3:+83852952 MS.gene75068:intron
GAAAGGGACTATGAGATCCA+TGG 0.576797 6.3:+83853801 MS.gene75068:CDS
TGCCTCCTGACAACTTCAAG+AGG 0.577185 6.3:-83853661 None:intergenic
GTGACCCTGCCATAAGGTCA+AGG 0.580517 6.3:-83853072 None:intergenic
ATAATGAACAAGTTTATCGA+GGG 0.581693 6.3:+83853313 MS.gene75068:CDS
CTTTGGAATATTCCCATACG+CGG 0.582745 6.3:+83853516 MS.gene75068:CDS
CTACCCTTGACCTTATGGCA+GGG 0.587881 6.3:+83853068 MS.gene75068:CDS
GGCATTAGAGGTCTGTATAG+GGG 0.588807 6.3:+83853372 MS.gene75068:CDS
TGGCAGGGTCACTCTTTGGA+GGG 0.591113 6.3:+83853083 MS.gene75068:CDS
AGAGCCGAGTTTCATACCAC+TGG 0.591866 6.3:+83852794 MS.gene75068:CDS
CAGTCCAGTGGTATGAAACT+CGG 0.592316 6.3:-83852798 None:intergenic
GCTAAAGAACTTCTTGCCGG+TGG 0.597460 6.3:+83851117 MS.gene75068:CDS
AATAATGAACAAGTTTATCG+AGG 0.610108 6.3:+83853312 MS.gene75068:CDS
GATACTCAGCCCTGAAAACA+GGG 0.610200 6.3:-83853854 None:intergenic
GGGAATATTCCAAAGAGTGT+CGG 0.616862 6.3:-83853508 None:intergenic
ATCATAAACTGTGAAGCCAA+TGG 0.621650 6.3:-83853996 None:intergenic
CTGACCCAATAAACCGGCCA+CGG 0.624628 6.3:-83853626 None:intergenic
GAAATGGAGCAAGTGTGGCA+AGG 0.627350 6.3:+83852957 MS.gene75068:CDS
TGACCCTGCCATAAGGTCAA+GGG 0.628759 6.3:-83853071 None:intergenic
GGATTACAAGAAAAGCATCA+TGG 0.645124 6.3:+83853585 MS.gene75068:CDS
TTTGGAATATTCCCATACGC+GGG 0.647148 6.3:+83853517 MS.gene75068:CDS
GAAACTGACATGTGGATCCG+TGG 0.653744 6.3:+83853609 MS.gene75068:CDS
TTAGTCCGAGTTAAGTCCTG+CGG 0.671738 6.3:-83853127 None:intergenic
ATCCTCTTGAAGTTGTCAGG+AGG 0.680873 6.3:+83853659 MS.gene75068:CDS
ATCATGGCGAAACTGACATG+TGG 0.686909 6.3:+83853601 MS.gene75068:CDS
TGACTAGGTTCAAAACCTTG+CGG 0.687829 6.3:+83853759 MS.gene75068:intron
CCTGCCATAAGGTCAAGGGT+AGG 0.694528 6.3:-83853067 None:intergenic
AAATGATCCAGACAGTCCAG+TGG 0.715090 6.3:-83852810 None:intergenic
AATGAAGCGACATGTACCTG+AGG 0.721078 6.3:+83853564 MS.gene75068:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCAAAAACTTATAAAGA+GGG + chr6.3:83853348-83853367 MS.gene75068:CDS 15.0%
!!! TTTTTCAAAAACTTATAAAG+AGG + chr6.3:83853347-83853366 MS.gene75068:CDS 15.0%
!! ACATGTATTGTAATAATTTC+AGG + chr6.3:83852436-83852455 MS.gene75068:intron 20.0%
!! TTGAATCATTGAAATTCAAA+GGG + chr6.3:83851401-83851420 MS.gene75068:intron 20.0%
!! TTTGAATTTCTTAAAGAAGA+CGG + chr6.3:83851440-83851459 MS.gene75068:intron 20.0%
!!! AAAAGTTTTGTTATACAGAA+CGG - chr6.3:83853934-83853953 None:intergenic 20.0%
!!! TTTTTTTGTTTTTGCAATTC+AGG + chr6.3:83853958-83853977 MS.gene75068:intron 20.0%
! AATAATGAACAAGTTTATCG+AGG + chr6.3:83853312-83853331 MS.gene75068:CDS 25.0%
! ACAAAATACAACCAAAATCA+TGG - chr6.3:83853206-83853225 None:intergenic 25.0%
! AGTATCAATTACATAAAGGT+GGG + chr6.3:83853871-83853890 MS.gene75068:intron 25.0%
! ATAATGAACAAGTTTATCGA+GGG + chr6.3:83853313-83853332 MS.gene75068:CDS 25.0%
! ATATCATTATTATCAGTGTC+CGG - chr6.3:83851241-83851260 None:intergenic 25.0%
! CTTGAATCATTGAAATTCAA+AGG + chr6.3:83851400-83851419 MS.gene75068:intron 25.0%
! GTAATCATATTTGTCAATGT+CGG + chr6.3:83852362-83852381 MS.gene75068:intron 25.0%
! TAACCTTATGTATGTAACTA+AGG - chr6.3:83851852-83851871 None:intergenic 25.0%
! TAATCATATTTGTCAATGTC+GGG + chr6.3:83852363-83852382 MS.gene75068:intron 25.0%
! TAGAACTCTTATGTTAATCA+TGG + chr6.3:83853477-83853496 MS.gene75068:intron 25.0%
!! ATAATTTCAGGTAAACATCT+AGG + chr6.3:83852448-83852467 MS.gene75068:intron 25.0%
!! TCAATTCTTATCCATGATTT+TGG + chr6.3:83853192-83853211 MS.gene75068:intron 25.0%
!! TTTGAAATTCTACTTCTATG+AGG + chr6.3:83853540-83853559 MS.gene75068:CDS 25.0%
!!! AAGTTTTTGAAAAACAGTCA+CGG - chr6.3:83853341-83853360 None:intergenic 25.0%
!!! AGGGAGGATTTTATTTATAA+AGG + chr6.3:83851797-83851816 MS.gene75068:intron 25.0%
!!! CATTGACACGTTTTTTTTTT+TGG - chr6.3:83851715-83851734 None:intergenic 25.0%
!!! CGTTTTTTTTTTTGGTTACA+AGG - chr6.3:83851707-83851726 None:intergenic 25.0%
AAGACTAACTAATGTGATCT+TGG + chr6.3:83851574-83851593 MS.gene75068:intron 30.0%
AGACACTATCATTACTTTAC+TGG - chr6.3:83852612-83852631 None:intergenic 30.0%
AGTGAAGAACGATAGTTATA+AGG - chr6.3:83852524-83852543 None:intergenic 30.0%
ATCAGCTAGTTAGTTAGTTA+CGG + chr6.3:83852144-83852163 MS.gene75068:intron 30.0%
ATGAAAAACATGAAAACTGC+TGG - chr6.3:83852555-83852574 None:intergenic 30.0%
ATTATTACTAGTGTGAGTGT+CGG - chr6.3:83851908-83851927 None:intergenic 30.0%
ATTCATGACAGTATTACAGA+AGG + chr6.3:83851777-83851796 MS.gene75068:intron 30.0%
CATATATGATTTACGCGTAT+CGG - chr6.3:83852325-83852344 None:intergenic 30.0%
CATTTAAATCTGCTTGTGTT+TGG + chr6.3:83853405-83853424 MS.gene75068:intron 30.0%
CTACCTTAGTTACATACATA+AGG + chr6.3:83851846-83851865 MS.gene75068:intron 30.0%
GAGTATCAATTACATAAAGG+TGG + chr6.3:83853870-83853889 MS.gene75068:intron 30.0%
GATACAAATTTAACCTGACT+AGG + chr6.3:83853744-83853763 MS.gene75068:intron 30.0%
GCTGAGTATCAATTACATAA+AGG + chr6.3:83853867-83853886 MS.gene75068:CDS 30.0%
GTGAAGAACGATAGTTATAA+GGG - chr6.3:83852523-83852542 None:intergenic 30.0%
TAGTTACATTTGCAGACATT+AGG + chr6.3:83851525-83851544 MS.gene75068:intron 30.0%
TCAAAAACTTATAAAGAGGG+TGG + chr6.3:83853351-83853370 MS.gene75068:CDS 30.0%
TTCATGACAGTATTACAGAA+GGG + chr6.3:83851778-83851797 MS.gene75068:intron 30.0%
!! GCTTTTTCTAATGTTTGGAA+AGG + chr6.3:83853043-83853062 MS.gene75068:CDS 30.0%
!! TGCAAGCTTTTTCTAATGTT+TGG + chr6.3:83853038-83853057 MS.gene75068:CDS 30.0%
AAACATCTAGGTAGTGTGTA+TGG + chr6.3:83852460-83852479 MS.gene75068:intron 35.0%
AAGCTTGCACAATCAATCTT+CGG - chr6.3:83853027-83853046 None:intergenic 35.0%
ACCAATGTGAAAATTTGCAG+GGG + chr6.3:83852935-83852954 MS.gene75068:intron 35.0%
ACGTCTCAAAATTCTCTTTC+AGG + chr6.3:83851170-83851189 MS.gene75068:CDS 35.0%
AGAAGTTCTTTAGCAAAGAG+AGG - chr6.3:83851111-83851130 None:intergenic 35.0%
AGCACACATACACTAAGAAT+GGG + chr6.3:83852221-83852240 MS.gene75068:intron 35.0%
ATAAAGTGAAGACCTGCAAA+AGG - chr6.3:83852989-83853008 None:intergenic 35.0%
ATCATAAACTGTGAAGCCAA+TGG - chr6.3:83853999-83854018 None:intergenic 35.0%
CAAACACTAACACAACACAT+CGG + chr6.3:83851622-83851641 MS.gene75068:intron 35.0%
CACCAATGTGAAAATTTGCA+GGG + chr6.3:83852934-83852953 MS.gene75068:intron 35.0%
CTTCGACACTAGTATTATAG+AGG + chr6.3:83852049-83852068 MS.gene75068:intron 35.0%
GAAGTTCTTTAGCAAAGAGA+GGG - chr6.3:83851110-83851129 None:intergenic 35.0%
GACACTAGTATTATAGAGGA+GGG + chr6.3:83852053-83852072 MS.gene75068:intron 35.0%
TAGCACACATACACTAAGAA+TGG + chr6.3:83852220-83852239 MS.gene75068:intron 35.0%
TCACCAATGTGAAAATTTGC+AGG + chr6.3:83852933-83852952 MS.gene75068:intron 35.0%
TCTCATAGTCCCTTTCAATT+CGG - chr6.3:83853797-83853816 None:intergenic 35.0%
TCTTCACTTTATGTCCTATG+AGG + chr6.3:83852997-83853016 MS.gene75068:CDS 35.0%
TGTCGGTACTTGACATAAAA+AGG - chr6.3:83851212-83851231 None:intergenic 35.0%
TTTGCTAAAGAACTTCTTGC+CGG + chr6.3:83851114-83851133 MS.gene75068:CDS 35.0%
! ATGCTTTTCTTGTAATCCTC+AGG - chr6.3:83853583-83853602 None:intergenic 35.0%
! CAACCAAGTTGAATGTTTCT+GGG + chr6.3:83853283-83853302 MS.gene75068:CDS 35.0%
! CTTTTGAGCAATCAAAACCA+TGG - chr6.3:83853821-83853840 None:intergenic 35.0%
!! CATCGGCACTGATAATAATT+TGG + chr6.3:83851639-83851658 MS.gene75068:intron 35.0%
!! GGATTACAAGAAAAGCATCA+TGG + chr6.3:83853585-83853604 MS.gene75068:CDS 35.0%
!!! AGAATTTTGAGACGTTCAAG+AGG - chr6.3:83851165-83851184 None:intergenic 35.0%
!!! GTTTTGATTGCTCAAAAGCA+AGG + chr6.3:83853823-83853842 MS.gene75068:CDS 35.0%
!!! TTTTGTTGTTGTGTCACTAG+TGG + chr6.3:83852675-83852694 MS.gene75068:intron 35.0%
AAAAAAGAAGCTCCGTAGCA+CGG - chr6.3:83851298-83851317 None:intergenic 40.0%
AGAATTTCAAACCCGCGTAT+GGG - chr6.3:83853531-83853550 None:intergenic 40.0%
AGTACCATCAAGAGATGAAG+TGG + chr6.3:83854039-83854058 MS.gene75068:CDS 40.0%
ATGACAGTATTACAGAAGGG+AGG + chr6.3:83851781-83851800 MS.gene75068:intron 40.0%
CAATCTTCGGTATTCCTCAT+AGG - chr6.3:83853014-83853033 None:intergenic 40.0%
CATATCCTCTTGAAGTTGTC+AGG + chr6.3:83853656-83853675 MS.gene75068:CDS 40.0%
CATATTTGTCAATGTCGGGT+TGG + chr6.3:83852367-83852386 MS.gene75068:intron 40.0%
CATCCCAGAAACATTCAACT+TGG - chr6.3:83853289-83853308 None:intergenic 40.0%
CCAGAAACATTCAACTTGGT+TGG - chr6.3:83853285-83853304 None:intergenic 40.0%
CGACACTAGTATTATAGAGG+AGG + chr6.3:83852052-83852071 MS.gene75068:intron 40.0%
CTGAAGAAGCCGAATTGAAA+GGG + chr6.3:83853785-83853804 MS.gene75068:CDS 40.0%
GAATGTCTGACCCAATAAAC+CGG - chr6.3:83853635-83853654 None:intergenic 40.0%
GTAGAATCCCAAAAGACCTT+CGG - chr6.3:83852852-83852871 None:intergenic 40.0%
GTGAAAATTTGCAGGGGAAA+TGG + chr6.3:83852941-83852960 MS.gene75068:intron 40.0%
GTTAGTTAGTTACGGACATG+AGG + chr6.3:83852152-83852171 MS.gene75068:intron 40.0%
TACAGGTAGATGGCAATCTT+AGG + chr6.3:83852869-83852888 MS.gene75068:intron 40.0%
TAGAATTTCAAACCCGCGTA+TGG - chr6.3:83853532-83853551 None:intergenic 40.0%
TAGTCCACTTCATCTCTTGA+TGG - chr6.3:83854046-83854065 None:intergenic 40.0%
TATAAAGAGGGTGGCATTAG+AGG + chr6.3:83853360-83853379 MS.gene75068:CDS 40.0%
TATAGACCCTACCTACCATA+GGG - chr6.3:83851553-83851572 None:intergenic 40.0%
TCTGAAGAAGCCGAATTGAA+AGG + chr6.3:83853784-83853803 MS.gene75068:CDS 40.0%
TGACTAGGTTCAAAACCTTG+CGG + chr6.3:83853759-83853778 MS.gene75068:intron 40.0%
TGGCATTAGAGGTCTGTATA+GGG + chr6.3:83853371-83853390 MS.gene75068:CDS 40.0%
TTAGTTACGGACATGAGGTA+AGG + chr6.3:83852157-83852176 MS.gene75068:intron 40.0%
! CCAACCAAGTTGAATGTTTC+TGG + chr6.3:83853282-83853301 MS.gene75068:CDS 40.0%
! GCAAGGATTATTCCTTTTGC+AGG + chr6.3:83852974-83852993 MS.gene75068:CDS 40.0%
! GGGAATATTCCAAAGAGTGT+CGG - chr6.3:83853511-83853530 None:intergenic 40.0%
! TCCCCTGCAAATTTTCACAT+TGG - chr6.3:83852939-83852958 None:intergenic 40.0%
! TCGGACTAAGTTAGCTTATC+AGG + chr6.3:83853141-83853160 MS.gene75068:CDS 40.0%
! TGATAGTGTCTAAGATTGCC+CGG + chr6.3:83852621-83852640 MS.gene75068:intron 40.0%
! TGCAGGTAACGTGCTTTATT+TGG + chr6.3:83853686-83853705 MS.gene75068:intron 40.0%
! TTTGGAATATTCCCATACGC+GGG + chr6.3:83853517-83853536 MS.gene75068:CDS 40.0%
! TTTGGGATTCTACAGGTAGA+TGG + chr6.3:83852859-83852878 MS.gene75068:intron 40.0%
!! AACAGTTTTAGCAAAGCCAC+CGG - chr6.3:83851136-83851155 None:intergenic 40.0%
!! AATTCAGGTTGTTCCATCTG+CGG + chr6.3:83853973-83853992 MS.gene75068:intron 40.0%
!! CAAGTTGAATGTTTCTGGGA+TGG + chr6.3:83853287-83853306 MS.gene75068:CDS 40.0%
!! CTTTGGAATATTCCCATACG+CGG + chr6.3:83853516-83853535 MS.gene75068:CDS 40.0%
!! GGTAACGTGCTTTATTTGGT+CGG + chr6.3:83853690-83853709 MS.gene75068:intron 40.0%
!! TGTGCTTTTTACTTATCCGC+AGG + chr6.3:83853111-83853130 MS.gene75068:CDS 40.0%
!!! AAGGTCTTTTGGGATTCTAC+AGG + chr6.3:83852852-83852871 MS.gene75068:CDS 40.0%
!!! GGAGGGCAACGTTTTTTTAA+AGG + chr6.3:83852070-83852089 MS.gene75068:intron 40.0%
AAATGATCCAGACAGTCCAG+TGG - chr6.3:83852813-83852832 None:intergenic 45.0%
AACGATTATCTCCCTACACC+CGG - chr6.3:83852643-83852662 None:intergenic 45.0%
AAGAATGGGCACGACATTGA+CGG + chr6.3:83852235-83852254 MS.gene75068:intron 45.0%
AATGAAGCGACATGTACCTG+AGG + chr6.3:83853564-83853583 MS.gene75068:CDS 45.0%
ACCAAGCACAGACACATACA+CGG + chr6.3:83851879-83851898 MS.gene75068:intron 45.0%
ATCATGGCGAAACTGACATG+TGG + chr6.3:83853601-83853620 MS.gene75068:CDS 45.0%
ATCCTCTTGAAGTTGTCAGG+AGG + chr6.3:83853659-83853678 MS.gene75068:CDS 45.0%
CAGTCCAGTGGTATGAAACT+CGG - chr6.3:83852801-83852820 None:intergenic 45.0%
CTTATCCGCAGGACTTAACT+CGG + chr6.3:83853122-83853141 MS.gene75068:CDS 45.0%
GAAAGGGACTATGAGATCCA+TGG + chr6.3:83853801-83853820 MS.gene75068:CDS 45.0%
GACATTAGGTACCCTATGGT+AGG + chr6.3:83851539-83851558 MS.gene75068:intron 45.0%
GATACTCAGCCCTGAAAACA+GGG - chr6.3:83853857-83853876 None:intergenic 45.0%
GGCATTAGAGGTCTGTATAG+GGG + chr6.3:83853372-83853391 MS.gene75068:CDS 45.0%
GGTAGTGTGTATGGAAGTGA+CGG + chr6.3:83852469-83852488 MS.gene75068:intron 45.0%
GGTCGGCTGGAAATTATAGA+TGG + chr6.3:83853707-83853726 MS.gene75068:intron 45.0%
GTATAGACCCTACCTACCAT+AGG - chr6.3:83851554-83851573 None:intergenic 45.0%
GTGGCATTAGAGGTCTGTAT+AGG + chr6.3:83853370-83853389 MS.gene75068:CDS 45.0%
TAGGTACCCTATGGTAGGTA+GGG + chr6.3:83851544-83851563 MS.gene75068:intron 45.0%
TGATACTCAGCCCTGAAAAC+AGG - chr6.3:83853858-83853877 None:intergenic 45.0%
TGATTGCTCAAAAGCAAGGC+TGG + chr6.3:83853827-83853846 MS.gene75068:CDS 45.0%
TGCAGACATTAGGTACCCTA+TGG + chr6.3:83851535-83851554 MS.gene75068:intron 45.0%
TGTAGCACAGACACTTCTAG+TGG - chr6.3:83852002-83852021 None:intergenic 45.0%
TTAGGTACCCTATGGTAGGT+AGG + chr6.3:83851543-83851562 MS.gene75068:intron 45.0%
TTAGTCCGAGTTAAGTCCTG+CGG - chr6.3:83853130-83853149 None:intergenic 45.0%
TTTCATACCACTGGACTGTC+TGG + chr6.3:83852803-83852822 MS.gene75068:CDS 45.0%
! ATGTCTTCCATGGGAAGTGT+CGG + chr6.3:83852409-83852428 MS.gene75068:intron 45.0%
! CATCTCTTGATGGTACTCTC+AGG - chr6.3:83854036-83854055 None:intergenic 45.0%
! GATAGTGTCTAAGATTGCCC+GGG + chr6.3:83852622-83852641 MS.gene75068:intron 45.0%
!! AATGTCGGGTTGGTACATGT+CGG + chr6.3:83852377-83852396 MS.gene75068:intron 45.0%
!! TCAAGTACCGACACAAACAC+CGG + chr6.3:83851219-83851238 MS.gene75068:intron 45.0%
!!! GCAAGGTTTTGAACCTAGTC+AGG - chr6.3:83853760-83853779 None:intergenic 45.0%
AATCAGACGTGTCCGTGCTA+CGG + chr6.3:83851283-83851302 MS.gene75068:intron 50.0%
ACGATTATCTCCCTACACCC+GGG - chr6.3:83852642-83852661 None:intergenic 50.0%
ACTCCGGACATGTCTTCCAT+GGG + chr6.3:83852400-83852419 MS.gene75068:intron 50.0%
AGAGCCGAGTTTCATACCAC+TGG + chr6.3:83852794-83852813 MS.gene75068:CDS 50.0%
AGAGGTCTGTATAGGGGAGT+AGG + chr6.3:83853378-83853397 MS.gene75068:CDS 50.0%
AGCAGAATTGCCAAGACCGA+AGG + chr6.3:83852833-83852852 MS.gene75068:CDS 50.0%
AGGTCCTACCCTTGACCTTA+TGG + chr6.3:83853063-83853082 MS.gene75068:CDS 50.0%
AGTTGTCAGGAGGCAAATGC+AGG + chr6.3:83853669-83853688 MS.gene75068:CDS 50.0%
CAAAGAGTGACCCTGCCATA+AGG - chr6.3:83853081-83853100 None:intergenic 50.0%
CTACCCTTGACCTTATGGCA+GGG + chr6.3:83853068-83853087 MS.gene75068:CDS 50.0%
CTTATGGCAGGGTCACTCTT+TGG + chr6.3:83853079-83853098 MS.gene75068:CDS 50.0%
CTTCCCATGGAAGACATGTC+CGG - chr6.3:83852406-83852425 None:intergenic 50.0%
GAAACTGACATGTGGATCCG+TGG + chr6.3:83853609-83853628 MS.gene75068:CDS 50.0%
GAAATGGAGCAAGTGTGGCA+AGG + chr6.3:83852957-83852976 MS.gene75068:CDS 50.0%
GAGAGGGATGTGATCGAGTA+TGG - chr6.3:83851094-83851113 None:intergenic 50.0%
GCTAAAGAACTTCTTGCCGG+TGG + chr6.3:83851117-83851136 MS.gene75068:CDS 50.0%
GGGTGTAGGGAGATAATCGT+TGG + chr6.3:83852642-83852661 MS.gene75068:intron 50.0%
GGTATGAAACTCGGCTCTTC+TGG - chr6.3:83852792-83852811 None:intergenic 50.0%
TCAGTGTCCGGTGTTTGTGT+CGG - chr6.3:83851229-83851248 None:intergenic 50.0%
TCCCAAAAGACCTTCGGTCT+TGG - chr6.3:83852846-83852865 None:intergenic 50.0%
TCCGTGTATGTGTCTGTGCT+TGG - chr6.3:83851883-83851902 None:intergenic 50.0%
TGACCCTGCCATAAGGTCAA+GGG - chr6.3:83853074-83853093 None:intergenic 50.0%
TGCCTCCTGACAACTTCAAG+AGG - chr6.3:83853664-83853683 None:intergenic 50.0%
! GCCAAGACCGAAGGTCTTTT+GGG + chr6.3:83852842-83852861 MS.gene75068:CDS 50.0%
! GCTGGAAGACCCTGTTTTCA+GGG + chr6.3:83853845-83853864 MS.gene75068:CDS 50.0%
! TCTAAGATTGCCCGGGTGTA+GGG + chr6.3:83852629-83852648 MS.gene75068:intron 50.0%
! TGCCAAGACCGAAGGTCTTT+TGG + chr6.3:83852841-83852860 MS.gene75068:CDS 50.0%
!! ACGTGCTTTATTTGGTCGGC+TGG + chr6.3:83853694-83853713 MS.gene75068:intron 50.0%
AATGGGCACGACATTGACGG+TGG + chr6.3:83852238-83852257 MS.gene75068:intron 55.0%
ATGGCAGGGTCACTCTTTGG+AGG + chr6.3:83853082-83853101 MS.gene75068:CDS 55.0%
CAGGGGAAATGGAGCAAGTG+TGG + chr6.3:83852952-83852971 MS.gene75068:intron 55.0%
CCTACCCTTGACCTTATGGC+AGG + chr6.3:83853067-83853086 MS.gene75068:CDS 55.0%
CCTGCCATAAGGTCAAGGGT+AGG - chr6.3:83853070-83853089 None:intergenic 55.0%
CTGACCCAATAAACCGGCCA+CGG - chr6.3:83853629-83853648 None:intergenic 55.0%
GACTCCGGACATGTCTTCCA+TGG + chr6.3:83852399-83852418 MS.gene75068:intron 55.0%
GTGACCCTGCCATAAGGTCA+AGG - chr6.3:83853075-83853094 None:intergenic 55.0%
GTTGTTCCATCTGCGGCCAT+TGG + chr6.3:83853980-83853999 MS.gene75068:CDS 55.0%
TGGCAGGGTCACTCTTTGGA+GGG + chr6.3:83853083-83853102 MS.gene75068:CDS 55.0%
! GGATCCGTGGCCGGTTTATT+GGG + chr6.3:83853622-83853641 MS.gene75068:CDS 55.0%
! GGCTGGAAGACCCTGTTTTC+AGG + chr6.3:83853844-83853863 MS.gene75068:CDS 55.0%
! GGTTGGTACATGTCGGACTC+CGG + chr6.3:83852384-83852403 MS.gene75068:intron 55.0%
! GTCTAAGATTGCCCGGGTGT+AGG + chr6.3:83852628-83852647 MS.gene75068:intron 55.0%
! TGGATCCGTGGCCGGTTTAT+TGG + chr6.3:83853621-83853640 MS.gene75068:CDS 55.0%
!! TCCAAAGAGTGTCGGAGCTG+TGG - chr6.3:83853503-83853522 None:intergenic 55.0%
!! TGTAGCACCGACACTTCCCA+TGG - chr6.3:83852419-83852438 None:intergenic 55.0%
CGGCTTCTTCAGATGCCGCA+AGG - chr6.3:83853777-83853796 None:intergenic 60.0%
CTGACATGTGGATCCGTGGC+CGG + chr6.3:83853613-83853632 MS.gene75068:CDS 60.0%
GCCACAGCTCCGACACTCTT+TGG + chr6.3:83853499-83853518 MS.gene75068:intron 60.0%
GTGAAGCCAATGGCCGCAGA+TGG - chr6.3:83853989-83854008 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 83851072 83854066 83851072 ID=MS.gene75068
chr6.3 mRNA 83851072 83854066 83851072 ID=MS.gene75068.t1;Parent=MS.gene75068
chr6.3 exon 83851072 83851191 83851072 ID=MS.gene75068.t1.exon1;Parent=MS.gene75068.t1
chr6.3 CDS 83851072 83851191 83851072 ID=cds.MS.gene75068.t1;Parent=MS.gene75068.t1
chr6.3 exon 83852788 83852873 83852788 ID=MS.gene75068.t1.exon2;Parent=MS.gene75068.t1
chr6.3 CDS 83852788 83852873 83852788 ID=cds.MS.gene75068.t1;Parent=MS.gene75068.t1
chr6.3 exon 83852955 83853162 83852955 ID=MS.gene75068.t1.exon3;Parent=MS.gene75068.t1
chr6.3 CDS 83852955 83853162 83852955 ID=cds.MS.gene75068.t1;Parent=MS.gene75068.t1
chr6.3 exon 83853273 83853399 83853273 ID=MS.gene75068.t1.exon4;Parent=MS.gene75068.t1
chr6.3 CDS 83853273 83853399 83853273 ID=cds.MS.gene75068.t1;Parent=MS.gene75068.t1
chr6.3 exon 83853506 83853690 83853506 ID=MS.gene75068.t1.exon5;Parent=MS.gene75068.t1
chr6.3 CDS 83853506 83853690 83853506 ID=cds.MS.gene75068.t1;Parent=MS.gene75068.t1
chr6.3 exon 83853766 83853888 83853766 ID=MS.gene75068.t1.exon6;Parent=MS.gene75068.t1
chr6.3 CDS 83853766 83853888 83853766 ID=cds.MS.gene75068.t1;Parent=MS.gene75068.t1
chr6.3 exon 83853980 83854066 83853980 ID=MS.gene75068.t1.exon7;Parent=MS.gene75068.t1
chr6.3 CDS 83853980 83854066 83853980 ID=cds.MS.gene75068.t1;Parent=MS.gene75068.t1
Gene Sequence

>MS.gene75068

ATGGCAGCATCATCAGAATCCATACTCGATCACATCCCTCTCTTTGCTAAAGAACTTCTTGCCGGTGGCTTTGCTAAAACTGTTGTTGCTCCTCTTGAACGTCTCAAAATTCTCTTTCAGGTACTTTCTTTAATTCTCCTTTTTATGTCAAGTACCGACACAAACACCGGACACTGATAATAATGATATGAAAAAAATGACATAATTCTTTAATCAGACGTGTCCGTGCTACGGAGCTTCTTTTTTCTTTTTTTATGTTTCTTCTTTGCTTTTTATTTTCGAAATGCATTTGTATGCTTTGATTTCATGCGTGATCTGTTTTTTGAATCTTGAATCATTGAAATTCAAAGGGAAATCTTATTTAGTCTTTTGAATTTCTTAAAGAAGACGGTAACTATTTATGAAGTTTAACTGCTTGAATTAACTCTAACTAACTCATGTCAATCAGTTAGTTAGTTACATTTGCAGACATTAGGTACCCTATGGTAGGTAGGGTCTATACAAGACTAACTAATGTGATCTTGGATACTATGAAGTGCAAGTGCAAATGCAAACACTAACACAACACATCGGCACTGATAATAATTTGGAAAAATGAATTAATTAAATGTAATCACATGTGTCAATGTTTGCCTTGTAACCAAAAAAAAAAACGTGTCAATGTTTGACACTGACACATGATTAATAAAAATCTTAGTATTTTGCATTCATGACAGTATTACAGAAGGGAGGATTTTATTTATAAAGGTAGCTGATGTAGTTTAACTGCTTAAACTACCTTAGTTACATACATAAGGTTATACTTATACCAAGCACAGACACATACACGGATGCCGACACTCACACTAGTAATAATTTGAGAAAATTAATTGAATGTAGTTACTTGTGTCGATGACAGTGACGCATGTCGAACACAGAACACATCTTCCACTAGAAGTGTCTGTGCTACATGTAATGTAATAATCACGAGTCTTTTTCTTCGACACTAGTATTATAGAGGAGGGCAACGTTTTTTTAAAGGCAGCTGATGCACTTAAGTACTGAAACTAACTCTAATAATTAACTCACTTGAATCAGCTAGTTAGTTAGTTACGGACATGAGGTAAGGTCTATATACAAGACTAGCTACTGTGATGTTGAATACTATGTAGCACACATACACTAAGAATGGGCACGACATTGACGGTGGCACGTCGACAACAATAATAATTTGAGAAAATGAGTTTAATCACATTTGTCAATGTCTGACACCGATACGCGTAAATCATATATGAAGACAATAAATTAATTGTAATCATATTTGTCAATGTCGGGTTGGTACATGTCGGACTCCGGACATGTCTTCCATGGGAAGTGTCGGTGCTACATGTATTGTAATAATTTCAGGTAAACATCTAGGTAGTGTGTATGGAAGTGACGGTGCTACATGTAATGTAATCATATTCTTTCCCTTATAACTATCGTTCTTCACTTGTCACTTCCAGCAGTTTTCATGTTTTTCATTTTTTCTTGTTCTTATTTTTCTGATATGCATACACCAGTAAAGTAATGATAGTGTCTAAGATTGCCCGGGTGTAGGGAGATAATCGTTGGAAACATGAAATTTTGTTGTTGTGTCACTAGTGGATGACTTTTGTTAAACTAGATTAGATGATCATAGATTGATAAATCACTATCATTATTTGCAGATTGATAAATCACTATCATTATTTGCAGACCAGAAGAGCCGAGTTTCATACCACTGGACTGTCTGGATCATTTAGCAGAATTGCCAAGACCGAAGGTCTTTTGGGATTCTACAGGTAGATGGCAATCTTAGGTGCTTTATCTTCTATCAATTAAATTAAATAATTAATCTGAATCACCAATGTGAAAATTTGCAGGGGAAATGGAGCAAGTGTGGCAAGGATTATTCCTTTTGCAGGTCTTCACTTTATGTCCTATGAGGAATACCGAAGATTGATTGTGCAAGCTTTTTCTAATGTTTGGAAAGGTCCTACCCTTGACCTTATGGCAGGGTCACTCTTTGGAGGGACAGCTGTGCTTTTTACTTATCCGCAGGACTTAACTCGGACTAAGTTAGCTTATCAGGTAAAGTAAATTTTTCATCAGAAATCAAATCAATTCTTATCCATGATTTTGGTTGTATTTTGTGATTACATGAAGTGTTAACTACACTGCATGTGAAATTGAAACTGCAGATTGTTAGTCCAACCAAGTTGAATGTTTCTGGGATGGTAAATAATGAACAAGTTTATCGAGGGATCCGTGACTGTTTTTCAAAAACTTATAAAGAGGGTGGCATTAGAGGTCTGTATAGGGGAGTAGGTATGCATTTAAATCTGCTTGTGTTTGGAACTGTTAATTCAGCAGCAGTATTGTATTTCACTGCTGTGTTGTTATTGTAGAACTCTTATGTTAATCATGGCCACAGCTCCGACACTCTTTGGAATATTCCCATACGCGGGTTTGAAATTCTACTTCTATGAGGAAATGAAGCGACATGTACCTGAGGATTACAAGAAAAGCATCATGGCGAAACTGACATGTGGATCCGTGGCCGGTTTATTGGGTCAGACATTCACATATCCTCTTGAAGTTGTCAGGAGGCAAATGCAGGTAACGTGCTTTATTTGGTCGGCTGGAAATTATAGATGGATATTGAGTAACATGATACAAATTTAACCTGACTAGGTTCAAAACCTTGCGGCATCTGAAGAAGCCGAATTGAAAGGGACTATGAGATCCATGGTTTTGATTGCTCAAAAGCAAGGCTGGAAGACCCTGTTTTCAGGGCTGAGTATCAATTACATAAAGGTGGGAAAATCATAGAACATTAAAGTAATTCACACTCTAGAACCGTTCTGTATAACAAAACTTTTTGTTTTTTTTTGTTTTTGCAATTCAGGTTGTTCCATCTGCGGCCATTGGCTTCACAGTTTATGATACTATGAAATCATACCTGAGAGTACCATCAAGAGATGAAGTGGACTAA
Protein sequence

>MS.gene75068.t1

MAASSESILDHIPLFAKELLAGGFAKTVVAPLERLKILFQTRRAEFHTTGLSGSFSRIAKTEGLLGFYRGNGASVARIIPFAGLHFMSYEEYRRLIVQAFSNVWKGPTLDLMAGSLFGGTAVLFTYPQDLTRTKLAYQIVSPTKLNVSGMVNNEQVYRGIRDCFSKTYKEGGIRGLYRGVAPTLFGIFPYAGLKFYFYEEMKRHVPEDYKKSIMAKLTCGSVAGLLGQTFTYPLEVVRRQMQVQNLAASEEAELKGTMRSMVLIAQKQGWKTLFSGLSINYIKVVPSAAIGFTVYDTMKSYLRVPSRDEVD