Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76710.t1 | KEH29195.1 | 87.9 | 116 | 14 | 0 | 1 | 116 | 58 | 173 | 6.50E-48 | 199.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76710.t1 | A0AT31 | 77.0 | 113 | 26 | 0 | 3 | 115 | 4 | 116 | 2.0e-42 | 172.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76710.t1 | A0A072UTH7 | 87.9 | 116 | 14 | 0 | 1 | 116 | 58 | 173 | 4.7e-48 | 199.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053209 | MS.gene76710 | 0.866788 | 2.21E-65 | -1.69E-46 |
| MS.gene056410 | MS.gene76710 | 0.802405 | 5.70E-49 | -1.69E-46 |
| MS.gene059129 | MS.gene76710 | 0.802074 | 6.66E-49 | -1.69E-46 |
| MS.gene059974 | MS.gene76710 | 0.856112 | 4.01E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene76710 | MS.gene82222 | PPI |
| MS.gene48831 | MS.gene76710 | PPI |
| MS.gene53305 | MS.gene76710 | PPI |
| MS.gene76710 | MS.gene48796 | PPI |
| MS.gene76710 | MS.gene53309 | PPI |
| MS.gene82256 | MS.gene76710 | PPI |
| MS.gene82221 | MS.gene76710 | PPI |
| MS.gene76710 | MS.gene48829 | PPI |
| MS.gene76710 | MS.gene76745 | PPI |
| MS.gene76710 | MS.gene76714 | PPI |
| MS.gene53311 | MS.gene76710 | PPI |
| MS.gene76713 | MS.gene76710 | PPI |
| MS.gene77025 | MS.gene76710 | PPI |
| MS.gene48835 | MS.gene76710 | PPI |
| MS.gene76705 | MS.gene76710 | PPI |
| MS.gene76710 | MS.gene48797 | PPI |
| MS.gene76710 | MS.gene82221 | PPI |
| MS.gene53312 | MS.gene76710 | PPI |
| MS.gene77022 | MS.gene76710 | PPI |
| MS.gene76710 | MS.gene82219 | PPI |
| MS.gene48828 | MS.gene76710 | PPI |
| MS.gene76710 | MS.gene76747 | PPI |
| MS.gene76710 | MS.gene82253 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76710.t1 | MTR_4g428370 | 87.931 | 116 | 14 | 0 | 1 | 116 | 58 | 173 | 1.25e-69 | 206 |
| MS.gene76710.t1 | MTR_4g027840 | 86.957 | 115 | 15 | 0 | 1 | 115 | 1 | 115 | 1.35e-66 | 197 |
| MS.gene76710.t1 | MTR_4g027800 | 86.207 | 116 | 16 | 0 | 1 | 116 | 1 | 116 | 1.72e-66 | 196 |
| MS.gene76710.t1 | MTR_4g029390 | 66.667 | 117 | 36 | 2 | 1 | 115 | 1 | 116 | 1.03e-47 | 149 |
| MS.gene76710.t1 | MTR_4g029350 | 66.667 | 117 | 36 | 2 | 1 | 115 | 1 | 116 | 1.24e-46 | 146 |
| MS.gene76710.t1 | MTR_4g028190 | 48.246 | 114 | 57 | 2 | 1 | 112 | 5 | 118 | 5.72e-31 | 107 |
| MS.gene76710.t1 | MTR_4g029210 | 48.214 | 112 | 58 | 0 | 1 | 112 | 1 | 112 | 2.13e-30 | 105 |
| MS.gene76710.t1 | MTR_7g072930 | 47.321 | 112 | 56 | 2 | 4 | 113 | 7 | 117 | 1.07e-28 | 102 |
| MS.gene76710.t1 | MTR_4g028180 | 43.220 | 118 | 60 | 3 | 5 | 115 | 9 | 126 | 2.91e-27 | 97.4 |
| MS.gene76710.t1 | MTR_7g072830 | 40.351 | 114 | 66 | 1 | 4 | 115 | 7 | 120 | 4.63e-27 | 97.1 |
| MS.gene76710.t1 | MTR_7g073150 | 45.690 | 116 | 58 | 4 | 2 | 113 | 3 | 117 | 4.94e-27 | 97.1 |
| MS.gene76710.t1 | MTR_7g072730 | 41.071 | 112 | 64 | 2 | 4 | 113 | 7 | 118 | 6.40e-26 | 95.5 |
| MS.gene76710.t1 | MTR_4g028310 | 40.336 | 119 | 68 | 1 | 1 | 116 | 1 | 119 | 5.60e-25 | 92.0 |
| MS.gene76710.t1 | MTR_7g073170 | 41.593 | 113 | 62 | 3 | 4 | 114 | 7 | 117 | 5.81e-25 | 91.7 |
| MS.gene76710.t1 | MTR_2g062600 | 41.739 | 115 | 65 | 2 | 1 | 114 | 1 | 114 | 1.17e-24 | 90.5 |
| MS.gene76710.t1 | MTR_7g073120 | 37.168 | 113 | 69 | 1 | 1 | 111 | 5 | 117 | 3.61e-21 | 82.8 |
| MS.gene76710.t1 | MTR_4g028250 | 39.831 | 118 | 68 | 2 | 1 | 115 | 1 | 118 | 6.59e-21 | 81.3 |
| MS.gene76710.t1 | MTR_7g072900 | 43.750 | 112 | 58 | 4 | 4 | 112 | 7 | 116 | 1.22e-20 | 80.5 |
| MS.gene76710.t1 | MTR_7g072993 | 42.857 | 112 | 59 | 3 | 4 | 111 | 7 | 117 | 8.94e-20 | 79.0 |
| MS.gene76710.t1 | MTR_3g467100 | 36.607 | 112 | 70 | 1 | 5 | 115 | 3 | 114 | 4.05e-19 | 76.6 |
| MS.gene76710.t1 | MTR_4g028360 | 36.283 | 113 | 69 | 3 | 4 | 114 | 1 | 112 | 1.23e-17 | 73.2 |
| MS.gene76710.t1 | MTR_7g072960 | 39.252 | 107 | 49 | 3 | 9 | 113 | 11 | 103 | 1.90e-17 | 72.8 |
| MS.gene76710.t1 | MTR_7g451500 | 38.043 | 92 | 56 | 1 | 24 | 114 | 7 | 98 | 3.34e-17 | 71.2 |
| MS.gene76710.t1 | MTR_7g072980 | 37.500 | 112 | 66 | 3 | 4 | 112 | 7 | 117 | 8.24e-17 | 71.2 |
| MS.gene76710.t1 | MTR_7g073130 | 38.739 | 111 | 64 | 4 | 4 | 112 | 7 | 115 | 1.08e-16 | 70.5 |
| MS.gene76710.t1 | MTR_1g040335 | 41.071 | 112 | 63 | 2 | 5 | 114 | 8 | 118 | 2.76e-16 | 69.3 |
| MS.gene76710.t1 | MTR_7g073060 | 38.053 | 113 | 67 | 3 | 4 | 114 | 7 | 118 | 7.07e-16 | 68.2 |
| MS.gene76710.t1 | MTR_7g073100 | 37.168 | 113 | 64 | 2 | 4 | 112 | 7 | 116 | 7.86e-16 | 68.9 |
| MS.gene76710.t1 | MTR_7g072810 | 39.091 | 110 | 59 | 3 | 4 | 111 | 7 | 110 | 5.80e-15 | 66.2 |
| MS.gene76710.t1 | MTR_3g056640 | 34.821 | 112 | 67 | 3 | 9 | 115 | 12 | 122 | 1.57e-14 | 65.1 |
| MS.gene76710.t1 | MTR_3g046515 | 34.821 | 112 | 67 | 4 | 3 | 110 | 10 | 119 | 9.26e-14 | 62.8 |
| MS.gene76710.t1 | MTR_3g046530 | 30.769 | 117 | 75 | 3 | 4 | 115 | 7 | 122 | 2.36e-13 | 62.0 |
| MS.gene76710.t1 | MTR_7g073030 | 35.455 | 110 | 68 | 3 | 4 | 111 | 7 | 115 | 2.96e-13 | 62.4 |
| MS.gene76710.t1 | MTR_3g464130 | 37.778 | 90 | 51 | 3 | 25 | 110 | 18 | 106 | 6.57e-13 | 60.5 |
| MS.gene76710.t1 | MTR_3g046350 | 31.373 | 102 | 64 | 3 | 10 | 106 | 13 | 113 | 1.66e-12 | 59.7 |
| MS.gene76710.t1 | MTR_3g046540 | 33.043 | 115 | 67 | 5 | 4 | 110 | 7 | 119 | 1.94e-12 | 59.7 |
| MS.gene76710.t1 | MTR_3g464110 | 40.244 | 82 | 44 | 3 | 33 | 110 | 92 | 172 | 2.54e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76710.t1 | AT5G59320 | 60.185 | 108 | 41 | 1 | 8 | 115 | 10 | 115 | 2.33e-39 | 127 |
| MS.gene76710.t1 | AT2G38540 | 63.793 | 116 | 36 | 6 | 4 | 115 | 5 | 118 | 2.80e-38 | 125 |
| MS.gene76710.t1 | AT5G59310 | 60.185 | 108 | 38 | 2 | 8 | 115 | 10 | 112 | 1.21e-37 | 123 |
| MS.gene76710.t1 | AT2G38530 | 61.739 | 115 | 40 | 4 | 4 | 115 | 5 | 118 | 1.46e-34 | 115 |
| MS.gene76710.t1 | AT3G51600 | 53.913 | 115 | 49 | 4 | 4 | 115 | 5 | 118 | 2.66e-30 | 105 |
| MS.gene76710.t1 | AT3G51590 | 47.273 | 110 | 55 | 3 | 6 | 114 | 8 | 115 | 7.40e-29 | 101 |
| MS.gene76710.t1 | AT2G15050 | 45.763 | 118 | 58 | 4 | 4 | 115 | 5 | 122 | 3.22e-28 | 100 |
| MS.gene76710.t1 | AT2G15050 | 45.690 | 116 | 57 | 4 | 4 | 113 | 5 | 120 | 8.99e-27 | 96.3 |
| MS.gene76710.t1 | AT2G15050 | 45.872 | 109 | 55 | 3 | 4 | 108 | 5 | 113 | 4.03e-26 | 94.4 |
| MS.gene76710.t1 | AT5G01870 | 44.737 | 114 | 55 | 4 | 9 | 115 | 4 | 116 | 7.80e-26 | 93.6 |
| MS.gene76710.t1 | AT4G33355 | 48.387 | 93 | 48 | 0 | 23 | 115 | 27 | 119 | 3.43e-24 | 89.7 |
| MS.gene76710.t1 | AT4G33355 | 49.438 | 89 | 45 | 0 | 23 | 111 | 27 | 115 | 1.37e-23 | 88.2 |
| MS.gene76710.t1 | AT3G08770 | 46.392 | 97 | 48 | 3 | 22 | 115 | 18 | 113 | 1.16e-21 | 83.2 |
| MS.gene76710.t1 | AT3G08770 | 46.316 | 95 | 47 | 3 | 22 | 113 | 18 | 111 | 9.52e-21 | 80.9 |
| MS.gene76710.t1 | AT2G18370 | 36.364 | 110 | 68 | 2 | 6 | 114 | 7 | 115 | 7.44e-19 | 75.9 |
| MS.gene76710.t1 | AT2G15325 | 35.165 | 91 | 56 | 2 | 27 | 115 | 31 | 120 | 2.26e-12 | 59.3 |
Find 31 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTGCTGCTGGTGCTATTTC+TGG | 0.136175 | 4.1:+71206272 | MS.gene76710:CDS |
| CCATGCATTGCATATCTTAG+AGG | 0.320070 | 4.1:+71206149 | MS.gene76710:CDS |
| AATGTTAACACCGCATTTGC+CGG | 0.346833 | 4.1:-71206324 | None:intergenic |
| CCATCTCCAGCATGCTGTGC+TGG | 0.375274 | 4.1:+71206182 | MS.gene76710:CDS |
| AACACAGCTGCTGGTCTTCC+CGG | 0.392611 | 4.1:+71206305 | MS.gene76710:CDS |
| TTGCAAGCAGCCTGACGGTC+AGG | 0.421711 | 4.1:-71206239 | None:intergenic |
| ATCTCATGTGGAACAGTAAC+TGG | 0.426922 | 4.1:+71206116 | MS.gene76710:CDS |
| GGTAGAGGTACTGATCTTGT+AGG | 0.430936 | 4.1:-71206348 | None:intergenic |
| CACCACCACGCCTGACCGTC+AGG | 0.443141 | 4.1:+71206229 | MS.gene76710:CDS |
| ACGGTCAGGCGTGGTGGTGG+CGG | 0.445777 | 4.1:-71206225 | None:intergenic |
| GTCAGGCGTGGTGGTGGCGG+CGG | 0.446691 | 4.1:-71206222 | None:intergenic |
| TTCACTCCAGCACAGCATGC+TGG | 0.485039 | 4.1:-71206188 | None:intergenic |
| AACTGCTTGAAAAGTGCTGC+TGG | 0.486030 | 4.1:+71206260 | MS.gene76710:CDS |
| ATGTTAACACCGCATTTGCC+GGG | 0.493990 | 4.1:-71206323 | None:intergenic |
| GCATATCTTAGAGGTGGTGC+TGG | 0.504129 | 4.1:+71206158 | MS.gene76710:CDS |
| CTGACGGTCAGGCGTGGTGG+TGG | 0.517462 | 4.1:-71206228 | None:intergenic |
| GTGCATGATCGTTGCACCTA+TGG | 0.527274 | 4.1:+71206082 | MS.gene76710:CDS |
| CCAGCACAGCATGCTGGAGA+TGG | 0.550113 | 4.1:-71206182 | None:intergenic |
| TTGAATGCTAACACAGCTGC+TGG | 0.551046 | 4.1:+71206296 | MS.gene76710:CDS |
| GTAGAGGTACTGATCTTGTA+GGG | 0.565695 | 4.1:-71206347 | None:intergenic |
| AGCAGCCTGACGGTCAGGCG+TGG | 0.565783 | 4.1:-71206234 | None:intergenic |
| TGGTTATATTTACAGCATCA+GGG | 0.568642 | 4.1:+71206825 | MS.gene76710:intron |
| AGCCTGACGGTCAGGCGTGG+TGG | 0.576862 | 4.1:-71206231 | None:intergenic |
| GAGATTGCAGCATCTGCCAT+AGG | 0.577723 | 4.1:-71206098 | None:intergenic |
| AAGAACTTACGTAGCACAGT+TGG | 0.585095 | 4.1:-71206369 | None:intergenic |
| CCTCTAAGATATGCAATGCA+TGG | 0.588805 | 4.1:-71206149 | None:intergenic |
| GCTGGTCTTCCCGGCAAATG+CGG | 0.592170 | 4.1:+71206314 | MS.gene76710:CDS |
| TTACGTAGCACAGTTGGTAG+AGG | 0.599761 | 4.1:-71206363 | None:intergenic |
| TGCATTGCATATCTTAGAGG+TGG | 0.623803 | 4.1:+71206152 | MS.gene76710:CDS |
| AGCAGTTGCAAGCAGCCTGA+CGG | 0.624471 | 4.1:-71206244 | None:intergenic |
| GCAGATGCTGCAATCTCATG+TGG | 0.654132 | 4.1:+71206104 | MS.gene76710:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTGGTTATTTATTTTT+TGG | + | chr4.1:71206731-71206750 | MS.gene76710:intron | 10.0% |
| !!! | AAACATTTTTGTGAATTTTT+TGG | + | chr4.1:71206650-71206669 | MS.gene76710:intron | 15.0% |
| !!! | TTATTTTTTGGTGTTATTAT+TGG | + | chr4.1:71206743-71206762 | MS.gene76710:intron | 15.0% |
| !!! | TTTTTCTTACTTCTAAAATT+TGG | - | chr4.1:71206588-71206607 | None:intergenic | 15.0% |
| !!! | GACTAGTTTTTTTTTTTGTT+TGG | + | chr4.1:71206805-71206824 | MS.gene76710:intron | 20.0% |
| !!! | TTATTGGTTAATATTCAAGT+CGG | + | chr4.1:71206759-71206778 | MS.gene76710:intron | 20.0% |
| GAAAAATATAGCAAGAGTGA+AGG | - | chr4.1:71206405-71206424 | None:intergenic | 30.0% | |
| TGGTTATATTTACAGCATCA+GGG | + | chr4.1:71206825-71206844 | MS.gene76710:intron | 30.0% | |
| TTAAGAAACGCTGTATAAAG+AGG | + | chr4.1:71206436-71206455 | MS.gene76710:intron | 30.0% | |
| TTGGTTATATTTACAGCATC+AGG | + | chr4.1:71206824-71206843 | MS.gene76710:intron | 30.0% | |
| CTGATCTACTGCTTCTTTAT+TGG | + | chr4.1:71206716-71206735 | MS.gene76710:intron | 35.0% | |
| AAGAACTTACGTAGCACAGT+TGG | - | chr4.1:71206372-71206391 | None:intergenic | 40.0% | |
| AATGTTAACACCGCATTTGC+CGG | - | chr4.1:71206327-71206346 | None:intergenic | 40.0% | |
| ATCTCATGTGGAACAGTAAC+TGG | + | chr4.1:71206116-71206135 | MS.gene76710:CDS | 40.0% | |
| CCATGCATTGCATATCTTAG+AGG | + | chr4.1:71206149-71206168 | MS.gene76710:CDS | 40.0% | |
| TATTCAAGTCGGTCGAAAAG+TGG | + | chr4.1:71206770-71206789 | MS.gene76710:intron | 40.0% | |
| TGCATTGCATATCTTAGAGG+TGG | + | chr4.1:71206152-71206171 | MS.gene76710:CDS | 40.0% | |
| !! | CCTCTAAGATATGCAATGCA+TGG | - | chr4.1:71206152-71206171 | None:intergenic | 40.0% |
| !! | GTAGAGGTACTGATCTTGTA+GGG | - | chr4.1:71206350-71206369 | None:intergenic | 40.0% |
| ATGTTAACACCGCATTTGCC+GGG | - | chr4.1:71206326-71206345 | None:intergenic | 45.0% | |
| TTGAATGCTAACACAGCTGC+TGG | + | chr4.1:71206296-71206315 | MS.gene76710:CDS | 45.0% | |
| !! | AACTGCTTGAAAAGTGCTGC+TGG | + | chr4.1:71206260-71206279 | MS.gene76710:CDS | 45.0% |
| !! | GGTAGAGGTACTGATCTTGT+AGG | - | chr4.1:71206351-71206370 | None:intergenic | 45.0% |
| !! | TTACGTAGCACAGTTGGTAG+AGG | - | chr4.1:71206366-71206385 | None:intergenic | 45.0% |
| !!! | AAAAATTATAATATTTTTAG+AGG | + | chr4.1:71206603-71206622 | MS.gene76710:intron | 5.0% |
| !!! | ACTTTATAATAATTTTTTTT+AGG | + | chr4.1:71206678-71206697 | MS.gene76710:intron | 5.0% |
| GAGATTGCAGCATCTGCCAT+AGG | - | chr4.1:71206101-71206120 | None:intergenic | 50.0% | |
| GCAGATGCTGCAATCTCATG+TGG | + | chr4.1:71206104-71206123 | MS.gene76710:CDS | 50.0% | |
| GCATATCTTAGAGGTGGTGC+TGG | + | chr4.1:71206158-71206177 | MS.gene76710:CDS | 50.0% | |
| GTGCATGATCGTTGCACCTA+TGG | + | chr4.1:71206082-71206101 | MS.gene76710:CDS | 50.0% | |
| !! | AGTGCTGCTGGTGCTATTTC+TGG | + | chr4.1:71206272-71206291 | MS.gene76710:CDS | 50.0% |
| AGCAGTTGCAAGCAGCCTGA+CGG | - | chr4.1:71206247-71206266 | None:intergenic | 55.0% | |
| TTCACTCCAGCACAGCATGC+TGG | - | chr4.1:71206191-71206210 | None:intergenic | 55.0% | |
| !! | AACACAGCTGCTGGTCTTCC+CGG | + | chr4.1:71206305-71206324 | MS.gene76710:CDS | 55.0% |
| CCAGCACAGCATGCTGGAGA+TGG | - | chr4.1:71206185-71206204 | None:intergenic | 60.0% | |
| CCATCTCCAGCATGCTGTGC+TGG | + | chr4.1:71206182-71206201 | MS.gene76710:CDS | 60.0% | |
| TTGCAAGCAGCCTGACGGTC+AGG | - | chr4.1:71206242-71206261 | None:intergenic | 60.0% | |
| !! | GCTGGTCTTCCCGGCAAATG+CGG | + | chr4.1:71206314-71206333 | MS.gene76710:CDS | 60.0% |
| AGCAGCCTGACGGTCAGGCG+TGG | - | chr4.1:71206237-71206256 | None:intergenic | 70.0% | |
| AGCCTGACGGTCAGGCGTGG+TGG | - | chr4.1:71206234-71206253 | None:intergenic | 70.0% | |
| CACCACCACGCCTGACCGTC+AGG | + | chr4.1:71206229-71206248 | MS.gene76710:CDS | 70.0% | |
| CTGACGGTCAGGCGTGGTGG+TGG | - | chr4.1:71206231-71206250 | None:intergenic | 70.0% | |
| !! | ACGGTCAGGCGTGGTGGTGG+CGG | - | chr4.1:71206228-71206247 | None:intergenic | 70.0% |
| !! | GTCAGGCGTGGTGGTGGCGG+CGG | - | chr4.1:71206225-71206244 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 71206044 | 71206852 | 71206044 | ID=MS.gene76710 |
| chr4.1 | mRNA | 71206044 | 71206852 | 71206044 | ID=MS.gene76710.t1;Parent=MS.gene76710 |
| chr4.1 | exon | 71206044 | 71206381 | 71206044 | ID=MS.gene76710.t1.exon1;Parent=MS.gene76710.t1 |
| chr4.1 | CDS | 71206044 | 71206381 | 71206044 | ID=cds.MS.gene76710.t1;Parent=MS.gene76710.t1 |
| chr4.1 | exon | 71206840 | 71206852 | 71206840 | ID=MS.gene76710.t1.exon2;Parent=MS.gene76710.t1 |
| chr4.1 | CDS | 71206840 | 71206852 | 71206840 | ID=cds.MS.gene76710.t1;Parent=MS.gene76710.t1 |
| Gene Sequence |
| Protein sequence |
