Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82256.t1 | XP_003605349.1 | 90.7 | 118 | 10 | 1 | 1 | 118 | 1 | 117 | 1.80E-50 | 208.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82256.t1 | A0AT28 | 66.1 | 118 | 40 | 0 | 1 | 118 | 1 | 118 | 2.6e-37 | 156.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82256.t1 | G7JJK0 | 90.7 | 118 | 10 | 1 | 1 | 118 | 1 | 117 | 1.3e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049151 | MS.gene82256 | 0.801083 | 1.06E-48 | -1.69E-46 |
| MS.gene049260 | MS.gene82256 | 0.860921 | 1.47E-63 | -1.69E-46 |
| MS.gene049346 | MS.gene82256 | 0.825163 | 5.45E-54 | -1.69E-46 |
| MS.gene049491 | MS.gene82256 | 0.82896 | 6.72E-55 | -1.69E-46 |
| MS.gene049614 | MS.gene82256 | 0.805357 | 1.39E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene82256 | 0.870458 | 1.44E-66 | -1.69E-46 |
| MS.gene050076 | MS.gene82256 | 0.821229 | 4.51E-53 | -1.69E-46 |
| MS.gene050099 | MS.gene82256 | 0.834692 | 2.59E-56 | -1.69E-46 |
| MS.gene050128 | MS.gene82256 | 0.878397 | 2.90E-69 | -1.69E-46 |
| MS.gene050301 | MS.gene82256 | 0.865239 | 6.82E-65 | -1.69E-46 |
| MS.gene050397 | MS.gene82256 | 0.817786 | 2.75E-52 | -1.69E-46 |
| MS.gene050404 | MS.gene82256 | 0.808442 | 3.09E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene82256 | 0.816769 | 4.66E-52 | -1.69E-46 |
| MS.gene050767 | MS.gene82256 | 0.808534 | 2.95E-50 | -1.69E-46 |
| MS.gene050770 | MS.gene82256 | 0.809558 | 1.78E-50 | -1.69E-46 |
| MS.gene05101 | MS.gene82256 | 0.847236 | 1.31E-59 | -1.69E-46 |
| MS.gene051471 | MS.gene82256 | 0.845733 | 3.38E-59 | -1.69E-46 |
| MS.gene051789 | MS.gene82256 | 0.813058 | 3.10E-51 | -1.69E-46 |
| MS.gene052078 | MS.gene82256 | 0.851 | 1.18E-60 | -1.69E-46 |
| MS.gene052178 | MS.gene82256 | 0.913743 | 4.32E-84 | -1.69E-46 |
| MS.gene052188 | MS.gene82256 | 0.807561 | 4.76E-50 | -1.69E-46 |
| MS.gene052287 | MS.gene82256 | 0.863076 | 3.22E-64 | -1.69E-46 |
| MS.gene052310 | MS.gene82256 | 0.816497 | 5.36E-52 | -1.69E-46 |
| MS.gene052326 | MS.gene82256 | 0.820495 | 6.66E-53 | -1.69E-46 |
| MS.gene052340 | MS.gene82256 | 0.842457 | 2.56E-58 | -1.69E-46 |
| MS.gene053073 | MS.gene82256 | 0.808197 | 3.49E-50 | -1.69E-46 |
| MS.gene053074 | MS.gene82256 | 0.801094 | 1.06E-48 | -1.69E-46 |
| MS.gene053204 | MS.gene82256 | 0.801003 | 1.11E-48 | -1.69E-46 |
| MS.gene053209 | MS.gene82256 | 0.849777 | 2.60E-60 | -1.69E-46 |
| MS.gene053557 | MS.gene82256 | 0.871213 | 8.11E-67 | -1.69E-46 |
| MS.gene053836 | MS.gene82256 | 0.844107 | 9.28E-59 | -1.69E-46 |
| MS.gene054010 | MS.gene82256 | 0.809705 | 1.66E-50 | -1.69E-46 |
| MS.gene054011 | MS.gene82256 | 0.824228 | 9.04E-54 | -1.69E-46 |
| MS.gene054012 | MS.gene82256 | 0.818867 | 1.57E-52 | -1.69E-46 |
| MS.gene054013 | MS.gene82256 | 0.818152 | 2.28E-52 | -1.69E-46 |
| MS.gene054184 | MS.gene82256 | 0.812094 | 5.04E-51 | -1.69E-46 |
| MS.gene054185 | MS.gene82256 | 0.814695 | 1.35E-51 | -1.69E-46 |
| MS.gene054214 | MS.gene82256 | 0.87094 | 9.98E-67 | -1.69E-46 |
| MS.gene054376 | MS.gene82256 | 0.814499 | 1.49E-51 | -1.69E-46 |
| MS.gene054618 | MS.gene82256 | 0.816334 | 5.83E-52 | -1.69E-46 |
| MS.gene055133 | MS.gene82256 | 0.823604 | 1.27E-53 | -1.69E-46 |
| MS.gene055254 | MS.gene82256 | 0.831583 | 1.54E-55 | -1.69E-46 |
| MS.gene055277 | MS.gene82256 | 0.887763 | 1.07E-72 | -1.69E-46 |
| MS.gene055303 | MS.gene82256 | 0.812682 | 3.75E-51 | -1.69E-46 |
| MS.gene055390 | MS.gene82256 | 0.856637 | 2.81E-62 | -1.69E-46 |
| MS.gene055422 | MS.gene82256 | 0.862215 | 5.93E-64 | -1.69E-46 |
| MS.gene055428 | MS.gene82256 | 0.839021 | 2.03E-57 | -1.69E-46 |
| MS.gene055604 | MS.gene82256 | 0.854924 | 8.90E-62 | -1.69E-46 |
| MS.gene056069 | MS.gene82256 | -0.806157 | 9.43E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene82256 | 0.82917 | 5.98E-55 | -1.69E-46 |
| MS.gene056314 | MS.gene82256 | 0.822786 | 1.97E-53 | -1.69E-46 |
| MS.gene056410 | MS.gene82256 | 0.806418 | 8.31E-50 | -1.69E-46 |
| MS.gene056609 | MS.gene82256 | 0.80695 | 6.41E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene82256 | 0.853389 | 2.47E-61 | -1.69E-46 |
| MS.gene056772 | MS.gene82256 | 0.852623 | 4.09E-61 | -1.69E-46 |
| MS.gene056931 | MS.gene82256 | 0.8175 | 3.19E-52 | -1.69E-46 |
| MS.gene056933 | MS.gene82256 | 0.815487 | 9.01E-52 | -1.69E-46 |
| MS.gene05702 | MS.gene82256 | 0.829043 | 6.42E-55 | -1.69E-46 |
| MS.gene057121 | MS.gene82256 | 0.803996 | 2.67E-49 | -1.69E-46 |
| MS.gene05733 | MS.gene82256 | 0.822661 | 2.10E-53 | -1.69E-46 |
| MS.gene057439 | MS.gene82256 | 0.821986 | 3.02E-53 | -1.69E-46 |
| MS.gene057448 | MS.gene82256 | 0.815512 | 8.90E-52 | -1.69E-46 |
| MS.gene057449 | MS.gene82256 | 0.813673 | 2.27E-51 | -1.69E-46 |
| MS.gene057450 | MS.gene82256 | 0.829035 | 6.45E-55 | -1.69E-46 |
| MS.gene057794 | MS.gene82256 | 0.817942 | 2.54E-52 | -1.69E-46 |
| MS.gene057796 | MS.gene82256 | 0.828383 | 9.27E-55 | -1.69E-46 |
| MS.gene058002 | MS.gene82256 | 0.848477 | 5.98E-60 | -1.69E-46 |
| MS.gene058082 | MS.gene82256 | 0.806829 | 6.80E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene82256 | 0.859081 | 5.29E-63 | -1.69E-46 |
| MS.gene058142 | MS.gene82256 | 0.800498 | 1.40E-48 | -1.69E-46 |
| MS.gene058293 | MS.gene82256 | 0.806267 | 8.94E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene82256 | 0.82845 | 8.93E-55 | -1.69E-46 |
| MS.gene058845 | MS.gene82256 | 0.809312 | 2.01E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene82256 | 0.817373 | 3.41E-52 | -1.69E-46 |
| MS.gene059228 | MS.gene82256 | 0.86849 | 6.28E-66 | -1.69E-46 |
| MS.gene059294 | MS.gene82256 | 0.830537 | 2.78E-55 | -1.69E-46 |
| MS.gene059326 | MS.gene82256 | 0.801717 | 7.89E-49 | -1.69E-46 |
| MS.gene059518 | MS.gene82256 | 0.856282 | 3.58E-62 | -1.69E-46 |
| MS.gene059597 | MS.gene82256 | 0.813232 | 2.84E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene82256 | 0.810427 | 1.16E-50 | -1.69E-46 |
| MS.gene059692 | MS.gene82256 | 0.838548 | 2.69E-57 | -1.69E-46 |
| MS.gene059695 | MS.gene82256 | 0.831527 | 1.59E-55 | -1.69E-46 |
| MS.gene059708 | MS.gene82256 | 0.811319 | 7.43E-51 | -1.69E-46 |
| MS.gene05984 | MS.gene82256 | 0.842362 | 2.71E-58 | -1.69E-46 |
| MS.gene059894 | MS.gene82256 | 0.888863 | 4.05E-73 | -1.69E-46 |
| MS.gene059974 | MS.gene82256 | 0.881921 | 1.61E-70 | -1.69E-46 |
| MS.gene060005 | MS.gene82256 | 0.85591 | 4.59E-62 | -1.69E-46 |
| MS.gene06037 | MS.gene82256 | 0.87239 | 3.30E-67 | -1.69E-46 |
| MS.gene060407 | MS.gene82256 | 0.804841 | 1.78E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene82256 | 0.861064 | 1.33E-63 | -1.69E-46 |
| MS.gene060559 | MS.gene82256 | 0.815089 | 1.11E-51 | -1.69E-46 |
| MS.gene060800 | MS.gene82256 | 0.816621 | 5.03E-52 | -1.69E-46 |
| MS.gene061049 | MS.gene82256 | 0.885264 | 9.45E-72 | -1.69E-46 |
| MS.gene061112 | MS.gene82256 | 0.855415 | 6.41E-62 | -1.69E-46 |
| MS.gene061113 | MS.gene82256 | 0.802197 | 6.29E-49 | -1.69E-46 |
| MS.gene061265 | MS.gene82256 | 0.848346 | 6.50E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene82256 | MS.gene82219 | PPI |
| MS.gene82256 | MS.gene76747 | PPI |
| MS.gene48831 | MS.gene82256 | PPI |
| MS.gene53305 | MS.gene82256 | PPI |
| MS.gene82256 | MS.gene76710 | PPI |
| MS.gene82256 | MS.gene77022 | PPI |
| MS.gene82256 | MS.gene82221 | PPI |
| MS.gene82256 | MS.gene48796 | PPI |
| MS.gene53311 | MS.gene82256 | PPI |
| MS.gene82256 | MS.gene82253 | PPI |
| MS.gene76713 | MS.gene82256 | PPI |
| MS.gene82256 | MS.gene48829 | PPI |
| MS.gene82256 | MS.gene76745 | PPI |
| MS.gene77025 | MS.gene82256 | PPI |
| MS.gene82256 | MS.gene82222 | PPI |
| MS.gene48835 | MS.gene82256 | PPI |
| MS.gene82256 | MS.gene53309 | PPI |
| MS.gene76705 | MS.gene82256 | PPI |
| MS.gene82256 | MS.gene76714 | PPI |
| MS.gene53312 | MS.gene82256 | PPI |
| MS.gene77022 | MS.gene82256 | PPI |
| MS.gene48828 | MS.gene82256 | PPI |
| MS.gene82256 | MS.gene48797 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82256.t1 | MTR_4g029390 | 90.678 | 118 | 10 | 1 | 1 | 118 | 1 | 117 | 2.35e-72 | 211 |
| MS.gene82256.t1 | MTR_4g029350 | 81.356 | 118 | 21 | 1 | 1 | 118 | 1 | 117 | 4.07e-65 | 193 |
| MS.gene82256.t1 | MTR_4g428370 | 70.085 | 117 | 33 | 1 | 1 | 117 | 58 | 172 | 3.11e-51 | 160 |
| MS.gene82256.t1 | MTR_4g027840 | 65.812 | 117 | 38 | 1 | 1 | 117 | 1 | 115 | 1.83e-48 | 151 |
| MS.gene82256.t1 | MTR_4g027800 | 64.957 | 117 | 39 | 1 | 1 | 117 | 1 | 115 | 2.57e-48 | 150 |
| MS.gene82256.t1 | MTR_4g029210 | 49.558 | 113 | 55 | 2 | 1 | 113 | 1 | 111 | 2.12e-28 | 100 |
| MS.gene82256.t1 | MTR_4g028180 | 48.246 | 114 | 51 | 4 | 11 | 117 | 14 | 126 | 1.35e-25 | 93.6 |
| MS.gene82256.t1 | MTR_2g062600 | 39.316 | 117 | 67 | 3 | 1 | 116 | 1 | 114 | 1.01e-23 | 88.2 |
| MS.gene82256.t1 | MTR_7g072930 | 44.860 | 107 | 55 | 3 | 11 | 115 | 13 | 117 | 1.49e-23 | 89.0 |
| MS.gene82256.t1 | MTR_7g072830 | 35.294 | 119 | 71 | 2 | 4 | 116 | 1 | 119 | 5.68e-22 | 84.0 |
| MS.gene82256.t1 | MTR_7g073170 | 40.367 | 109 | 62 | 3 | 11 | 118 | 13 | 119 | 8.54e-22 | 83.6 |
| MS.gene82256.t1 | MTR_7g073150 | 43.860 | 114 | 59 | 4 | 4 | 115 | 7 | 117 | 1.45e-21 | 83.2 |
| MS.gene82256.t1 | MTR_4g028190 | 37.815 | 119 | 72 | 2 | 1 | 118 | 5 | 122 | 5.55e-21 | 81.6 |
| MS.gene82256.t1 | MTR_3g467100 | 33.628 | 113 | 74 | 1 | 5 | 117 | 3 | 114 | 2.01e-20 | 79.7 |
| MS.gene82256.t1 | MTR_7g072730 | 34.746 | 118 | 71 | 2 | 4 | 115 | 1 | 118 | 3.01e-20 | 80.9 |
| MS.gene82256.t1 | MTR_4g028250 | 35.593 | 118 | 75 | 1 | 1 | 117 | 1 | 118 | 2.89e-19 | 77.0 |
| MS.gene82256.t1 | MTR_7g073120 | 35.345 | 116 | 71 | 3 | 1 | 114 | 5 | 118 | 1.46e-16 | 70.9 |
| MS.gene82256.t1 | MTR_7g072993 | 37.069 | 116 | 70 | 1 | 2 | 114 | 3 | 118 | 3.13e-16 | 69.7 |
| MS.gene82256.t1 | MTR_4g028310 | 32.500 | 120 | 76 | 3 | 1 | 117 | 1 | 118 | 1.36e-15 | 68.2 |
| MS.gene82256.t1 | MTR_3g046350 | 33.645 | 107 | 64 | 4 | 12 | 115 | 15 | 117 | 3.44e-15 | 66.6 |
| MS.gene82256.t1 | MTR_3g056640 | 31.967 | 122 | 77 | 4 | 1 | 117 | 2 | 122 | 5.29e-15 | 66.2 |
| MS.gene82256.t1 | MTR_7g072980 | 32.174 | 115 | 76 | 1 | 2 | 114 | 3 | 117 | 7.22e-15 | 66.2 |
| MS.gene82256.t1 | MTR_7g073130 | 34.211 | 114 | 73 | 2 | 2 | 114 | 3 | 115 | 1.63e-14 | 65.1 |
| MS.gene82256.t1 | MTR_3g046530 | 29.915 | 117 | 78 | 3 | 4 | 117 | 7 | 122 | 2.39e-14 | 64.7 |
| MS.gene82256.t1 | MTR_7g451500 | 33.696 | 92 | 60 | 1 | 26 | 116 | 7 | 98 | 2.94e-14 | 63.5 |
| MS.gene82256.t1 | MTR_7g072900 | 35.088 | 114 | 73 | 1 | 2 | 114 | 3 | 116 | 3.35e-14 | 63.9 |
| MS.gene82256.t1 | MTR_3g046515 | 30.435 | 115 | 76 | 3 | 4 | 115 | 7 | 120 | 4.45e-14 | 63.9 |
| MS.gene82256.t1 | MTR_3g046540 | 29.915 | 117 | 78 | 3 | 4 | 117 | 7 | 122 | 6.29e-14 | 63.5 |
| MS.gene82256.t1 | MTR_1g040335 | 34.454 | 119 | 71 | 3 | 4 | 116 | 1 | 118 | 6.90e-14 | 63.2 |
| MS.gene82256.t1 | MTR_7g072960 | 38.596 | 114 | 51 | 5 | 4 | 115 | 7 | 103 | 7.79e-14 | 63.5 |
| MS.gene82256.t1 | MTR_3g464130 | 35.556 | 90 | 55 | 2 | 27 | 114 | 18 | 106 | 2.89e-13 | 61.2 |
| MS.gene82256.t1 | MTR_3g464110 | 37.805 | 82 | 48 | 2 | 36 | 115 | 93 | 173 | 1.85e-12 | 60.8 |
| MS.gene82256.t1 | MTR_7g072810 | 36.449 | 107 | 59 | 3 | 11 | 115 | 13 | 112 | 1.95e-12 | 59.7 |
| MS.gene82256.t1 | MTR_4g028360 | 33.913 | 115 | 71 | 4 | 4 | 116 | 1 | 112 | 4.41e-12 | 58.9 |
| MS.gene82256.t1 | MTR_7g073060 | 32.759 | 116 | 77 | 1 | 2 | 116 | 3 | 118 | 1.85e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82256.t1 | AT5G59320 | 47.273 | 110 | 57 | 1 | 8 | 117 | 7 | 115 | 1.45e-28 | 100 |
| MS.gene82256.t1 | AT5G59310 | 46.364 | 110 | 55 | 2 | 8 | 117 | 7 | 112 | 1.43e-26 | 95.5 |
| MS.gene82256.t1 | AT3G51590 | 45.370 | 108 | 56 | 1 | 12 | 116 | 8 | 115 | 2.22e-25 | 92.8 |
| MS.gene82256.t1 | AT2G38540 | 48.276 | 116 | 56 | 4 | 4 | 117 | 5 | 118 | 3.31e-25 | 92.4 |
| MS.gene82256.t1 | AT3G51600 | 49.074 | 108 | 52 | 3 | 12 | 117 | 12 | 118 | 2.18e-24 | 90.1 |
| MS.gene82256.t1 | AT2G38530 | 46.087 | 115 | 60 | 2 | 4 | 117 | 5 | 118 | 3.92e-24 | 89.4 |
| MS.gene82256.t1 | AT5G01870 | 43.590 | 117 | 61 | 4 | 4 | 117 | 2 | 116 | 2.93e-23 | 87.4 |
| MS.gene82256.t1 | AT4G33355 | 43.519 | 108 | 57 | 1 | 13 | 116 | 11 | 118 | 3.21e-23 | 87.4 |
| MS.gene82256.t1 | AT4G33355 | 42.453 | 106 | 57 | 1 | 13 | 114 | 11 | 116 | 5.86e-22 | 84.0 |
| MS.gene82256.t1 | AT3G08770 | 44.330 | 97 | 50 | 3 | 24 | 117 | 18 | 113 | 1.63e-20 | 80.1 |
| MS.gene82256.t1 | AT3G08770 | 44.211 | 95 | 49 | 3 | 24 | 115 | 18 | 111 | 1.83e-19 | 77.4 |
| MS.gene82256.t1 | AT2G15050 | 39.286 | 112 | 62 | 4 | 12 | 117 | 11 | 122 | 3.90e-19 | 77.0 |
| MS.gene82256.t1 | AT2G15050 | 39.091 | 110 | 61 | 4 | 12 | 115 | 11 | 120 | 9.43e-18 | 73.6 |
| MS.gene82256.t1 | AT2G15050 | 39.806 | 103 | 58 | 3 | 12 | 110 | 11 | 113 | 2.99e-17 | 72.0 |
| MS.gene82256.t1 | AT2G18370 | 35.789 | 95 | 59 | 2 | 23 | 116 | 22 | 115 | 9.33e-16 | 68.2 |
Find 35 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACTTCTACTAACTGTAAT+AGG | 0.249879 | 4.2:+74770948 | MS.gene82256:CDS |
| CTTGCGGGCGGGGCCGTTGT+TGG | 0.344393 | 4.2:-74770816 | None:intergenic |
| GCAGTTGCAAGCAGTCTTGC+GGG | 0.361401 | 4.2:-74770831 | None:intergenic |
| TGCAAGCAGTCTTGCGGGCG+GGG | 0.373033 | 4.2:-74770826 | None:intergenic |
| CCATGCATTACTTATCTTCA+AGG | 0.382464 | 4.2:+74770737 | MS.gene82256:CDS |
| AGTAGAAGTGCTAAACTTGT+AGG | 0.409303 | 4.2:-74770936 | None:intergenic |
| TTGCAAGCAGTCTTGCGGGC+GGG | 0.409587 | 4.2:-74770827 | None:intergenic |
| ACTTATCTTCAAGGTGGTGC+TGG | 0.410647 | 4.2:+74770746 | MS.gene82256:CDS |
| GTGACTGCTGCTTCTGCCAT+TGG | 0.422408 | 4.2:-74770686 | None:intergenic |
| GATAAGTAATGCATGGAGTA+AGG | 0.447792 | 4.2:-74770730 | None:intergenic |
| GCGGGCGGGGCCGTTGTTGG+CGG | 0.458612 | 4.2:-74770813 | None:intergenic |
| TGCAATTATGTGCATGGTGG+TGG | 0.466945 | 4.2:+74770655 | MS.gene82256:CDS |
| AACTGCTTGAAAAGTGCTGC+TGG | 0.487551 | 4.2:+74770848 | MS.gene82256:CDS |
| ATGTTAACACCACATTTGCC+AGG | 0.490963 | 4.2:-74770911 | None:intergenic |
| GCTGCTCTCCCTGGCAAATG+TGG | 0.495611 | 4.2:+74770902 | MS.gene82256:CDS |
| AGCAGTTGCAAGCAGTCTTG+CGG | 0.506600 | 4.2:-74770832 | None:intergenic |
| GTTGTTATTCAAACGAGCAA+TGG | 0.508618 | 4.2:-74770873 | None:intergenic |
| AACCAAGCTGCTGCTCTCCC+TGG | 0.514408 | 4.2:+74770893 | MS.gene82256:CDS |
| GGTGGAGTGAGGAGACTTAA+CGG | 0.519956 | 4.2:+74770788 | MS.gene82256:CDS |
| TGCCAGGGAGAGCAGCAGCT+TGG | 0.525683 | 4.2:-74770895 | None:intergenic |
| TTTGGCTGCAATTATGTGCA+TGG | 0.538143 | 4.2:+74770649 | MS.gene82256:CDS |
| GGTCCATCTGCAGCATGCTG+TGG | 0.539612 | 4.2:+74770767 | MS.gene82256:CDS |
| GTTGCAAGCAGTCTTGCGGG+CGG | 0.541246 | 4.2:-74770828 | None:intergenic |
| CCATCTGCAGCATGCTGTGG+TGG | 0.550747 | 4.2:+74770770 | MS.gene82256:CDS |
| GTAGAAGTGCTAAACTTGTA+GGG | 0.551668 | 4.2:-74770935 | None:intergenic |
| GTCACATGTGGACAAGTAGT+AGG | 0.553840 | 4.2:+74770704 | MS.gene82256:CDS |
| CCACCACAGCATGCTGCAGA+TGG | 0.580113 | 4.2:-74770770 | None:intergenic |
| GGTGGTGGTTACTGCACCAA+TGG | 0.601083 | 4.2:+74770670 | MS.gene82256:CDS |
| CTTAACGGTGCCGCCAACAA+CGG | 0.605014 | 4.2:+74770803 | MS.gene82256:CDS |
| CCTTGAAGATAAGTAATGCA+TGG | 0.607580 | 4.2:-74770737 | None:intergenic |
| GCAGAAGCAGCAGTCACATG+TGG | 0.630368 | 4.2:+74770692 | MS.gene82256:CDS |
| TGTTAACACCACATTTGCCA+GGG | 0.642773 | 4.2:-74770910 | None:intergenic |
| CAGCATGCTGTGGTGGAGTG+AGG | 0.648040 | 4.2:+74770777 | MS.gene82256:CDS |
| TGCATTACTTATCTTCAAGG+TGG | 0.657751 | 4.2:+74770740 | MS.gene82256:CDS |
| GGCTGCAATTATGTGCATGG+TGG | 0.657959 | 4.2:+74770652 | MS.gene82256:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTTATTTTTTTTTAAAA+TGG | + | chr4.2:74771320-74771339 | MS.gene82256:intron | 0.0% |
| !! | ATTTCTCATATACGTAATTA+AGG | - | chr4.2:74770992-74771011 | None:intergenic | 20.0% |
| !! | GGCAAATTTAATAAGTTATT+CGG | - | chr4.2:74771281-74771300 | None:intergenic | 20.0% |
| ! | ACACACACATACATATATAT+AGG | - | chr4.2:74771184-74771203 | None:intergenic | 25.0% |
| ! | ATTTGCCTAAAAAAATGAAG+TGG | + | chr4.2:74771294-74771313 | MS.gene82256:intron | 25.0% |
| ! | CACACACATACATATATATA+GGG | - | chr4.2:74771183-74771202 | None:intergenic | 25.0% |
| ! | TGAGACATTGAAATGATAAT+TGG | + | chr4.2:74771440-74771459 | MS.gene82256:intron | 25.0% |
| !! | TCTCTAAGAATACTCTTTAA+CGG | + | chr4.2:74771022-74771041 | MS.gene82256:intron | 25.0% |
| !!! | AATCTTTAAAGAGTACCATT+AGG | + | chr4.2:74771147-74771166 | MS.gene82256:intron | 25.0% |
| !!! | ATCTTTAAAGAGTACCATTA+GGG | + | chr4.2:74771148-74771167 | MS.gene82256:intron | 25.0% |
| !!! | TTGTTCCACTTCATTTTTTT+AGG | - | chr4.2:74771302-74771321 | None:intergenic | 25.0% |
| AACTATCTATATGCTCATGA+AGG | - | chr4.2:74771696-74771715 | None:intergenic | 30.0% | |
| ACTATCTATATGCTCATGAA+GGG | - | chr4.2:74771695-74771714 | None:intergenic | 30.0% | |
| ATTGAAATGATAATTGGAGG+AGG | + | chr4.2:74771446-74771465 | MS.gene82256:intron | 30.0% | |
| GACATTGAAATGATAATTGG+AGG | + | chr4.2:74771443-74771462 | MS.gene82256:intron | 30.0% | |
| ! | TTGAGTGACTTATGCATTTT+TGG | + | chr4.2:74771562-74771581 | MS.gene82256:intron | 30.0% |
| ! | TTTTTGGACAGTCATCAAAA+TGG | + | chr4.2:74771578-74771597 | MS.gene82256:intron | 30.0% |
| !! | TATTTTTGTTCCACTTCTTC+AGG | - | chr4.2:74771258-74771277 | None:intergenic | 30.0% |
| CCATGCATTACTTATCTTCA+AGG | + | chr4.2:74770737-74770756 | MS.gene82256:CDS | 35.0% | |
| CCTTGAAGATAAGTAATGCA+TGG | - | chr4.2:74770740-74770759 | None:intergenic | 35.0% | |
| GATAAGTAATGCATGGAGTA+AGG | - | chr4.2:74770733-74770752 | None:intergenic | 35.0% | |
| GCACTTCTACTAACTGTAAT+AGG | + | chr4.2:74770948-74770967 | MS.gene82256:CDS | 35.0% | |
| TGCATTACTTATCTTCAAGG+TGG | + | chr4.2:74770740-74770759 | MS.gene82256:CDS | 35.0% | |
| ! | AAGCATGAGAGTTGCATTTT+TGG | + | chr4.2:74770631-74770650 | MS.gene82256:CDS | 35.0% |
| ! | GTCTCACTCTCACTTAATTT+TGG | - | chr4.2:74771426-74771445 | None:intergenic | 35.0% |
| ! | GTTGTTATTCAAACGAGCAA+TGG | - | chr4.2:74770876-74770895 | None:intergenic | 35.0% |
| !! | AGTAGAAGTGCTAAACTTGT+AGG | - | chr4.2:74770939-74770958 | None:intergenic | 35.0% |
| !! | GTAGAAGTGCTAAACTTGTA+GGG | - | chr4.2:74770938-74770957 | None:intergenic | 35.0% |
| !!! | TCATCATCTTTTTAGCTTGG+AGG | - | chr4.2:74771403-74771422 | None:intergenic | 35.0% |
| !!! | TGGTCATCATCTTTTTAGCT+TGG | - | chr4.2:74771406-74771425 | None:intergenic | 35.0% |
| ATCTCAAGTACCTGAAGAAG+TGG | + | chr4.2:74771245-74771264 | MS.gene82256:intron | 40.0% | |
| ATGTTAACACCACATTTGCC+AGG | - | chr4.2:74770914-74770933 | None:intergenic | 40.0% | |
| TGTTAACACCACATTTGCCA+GGG | - | chr4.2:74770913-74770932 | None:intergenic | 40.0% | |
| TTTGGCTGCAATTATGTGCA+TGG | + | chr4.2:74770649-74770668 | MS.gene82256:CDS | 40.0% | |
| ! | CTTTAACGGTCTAAAGTAGC+AGG | + | chr4.2:74771036-74771055 | MS.gene82256:intron | 40.0% |
| ! | GTTTTCTGTATTGCAGCATC+AGG | + | chr4.2:74771773-74771792 | MS.gene82256:intron | 40.0% |
| !! | TAGGGTTTTGTGTTCCCTAA+TGG | - | chr4.2:74771165-74771184 | None:intergenic | 40.0% |
| ACTTATCTTCAAGGTGGTGC+TGG | + | chr4.2:74770746-74770765 | MS.gene82256:CDS | 45.0% | |
| GTCACATGTGGACAAGTAGT+AGG | + | chr4.2:74770704-74770723 | MS.gene82256:CDS | 45.0% | |
| TGCAATTATGTGCATGGTGG+TGG | + | chr4.2:74770655-74770674 | MS.gene82256:CDS | 45.0% | |
| !! | AACTGCTTGAAAAGTGCTGC+TGG | + | chr4.2:74770848-74770867 | MS.gene82256:CDS | 45.0% |
| !!! | TTTATATTTTATAAAATAAG+TGG | + | chr4.2:74771530-74771549 | MS.gene82256:intron | 5.0% |
| AGCAGTTGCAAGCAGTCTTG+CGG | - | chr4.2:74770835-74770854 | None:intergenic | 50.0% | |
| GGCTGCAATTATGTGCATGG+TGG | + | chr4.2:74770652-74770671 | MS.gene82256:CDS | 50.0% | |
| GGTGGAGTGAGGAGACTTAA+CGG | + | chr4.2:74770788-74770807 | MS.gene82256:CDS | 50.0% | |
| GCAGAAGCAGCAGTCACATG+TGG | + | chr4.2:74770692-74770711 | MS.gene82256:CDS | 55.0% | |
| GCAGTTGCAAGCAGTCTTGC+GGG | - | chr4.2:74770834-74770853 | None:intergenic | 55.0% | |
| ! | GGTGGTGGTTACTGCACCAA+TGG | + | chr4.2:74770670-74770689 | MS.gene82256:CDS | 55.0% |
| ! | GTGACTGCTGCTTCTGCCAT+TGG | - | chr4.2:74770689-74770708 | None:intergenic | 55.0% |
| !! | CTTAACGGTGCCGCCAACAA+CGG | + | chr4.2:74770803-74770822 | MS.gene82256:CDS | 55.0% |
| AACCAAGCTGCTGCTCTCCC+TGG | + | chr4.2:74770893-74770912 | MS.gene82256:CDS | 60.0% | |
| CCACCACAGCATGCTGCAGA+TGG | - | chr4.2:74770773-74770792 | None:intergenic | 60.0% | |
| CCATCTGCAGCATGCTGTGG+TGG | + | chr4.2:74770770-74770789 | MS.gene82256:CDS | 60.0% | |
| GCTGCTCTCCCTGGCAAATG+TGG | + | chr4.2:74770902-74770921 | MS.gene82256:CDS | 60.0% | |
| GGTCCATCTGCAGCATGCTG+TGG | + | chr4.2:74770767-74770786 | MS.gene82256:CDS | 60.0% | |
| GTTGCAAGCAGTCTTGCGGG+CGG | - | chr4.2:74770831-74770850 | None:intergenic | 60.0% | |
| TTGCAAGCAGTCTTGCGGGC+GGG | - | chr4.2:74770830-74770849 | None:intergenic | 60.0% | |
| ! | CAGCATGCTGTGGTGGAGTG+AGG | + | chr4.2:74770777-74770796 | MS.gene82256:CDS | 60.0% |
| TGCAAGCAGTCTTGCGGGCG+GGG | - | chr4.2:74770829-74770848 | None:intergenic | 65.0% | |
| TGCCAGGGAGAGCAGCAGCT+TGG | - | chr4.2:74770898-74770917 | None:intergenic | 65.0% | |
| CTTGCGGGCGGGGCCGTTGT+TGG | - | chr4.2:74770819-74770838 | None:intergenic | 75.0% | |
| ! | GCGGGCGGGGCCGTTGTTGG+CGG | - | chr4.2:74770816-74770835 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 74770626 | 74771801 | 74770626 | ID=MS.gene82256 |
| chr4.2 | mRNA | 74770626 | 74771801 | 74770626 | ID=MS.gene82256.t1;Parent=MS.gene82256 |
| chr4.2 | exon | 74770626 | 74770969 | 74770626 | ID=MS.gene82256.t1.exon1;Parent=MS.gene82256.t1 |
| chr4.2 | CDS | 74770626 | 74770969 | 74770626 | ID=cds.MS.gene82256.t1;Parent=MS.gene82256.t1 |
| chr4.2 | exon | 74771789 | 74771801 | 74771789 | ID=MS.gene82256.t1.exon2;Parent=MS.gene82256.t1 |
| chr4.2 | CDS | 74771789 | 74771801 | 74771789 | ID=cds.MS.gene82256.t1;Parent=MS.gene82256.t1 |
| Gene Sequence |
| Protein sequence |
