Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48796.t1 | XP_003605349.1 | 91.5 | 118 | 9 | 1 | 1 | 118 | 1 | 117 | 1.10E-50 | 209.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48796.t1 | A0AT28 | 65.3 | 118 | 41 | 0 | 1 | 118 | 1 | 118 | 5.8e-37 | 154.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48796.t1 | G7JJK0 | 91.5 | 118 | 9 | 1 | 1 | 118 | 1 | 117 | 7.8e-51 | 209.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049151 | MS.gene48796 | 0.804478 | 2.12E-49 | -1.69E-46 |
| MS.gene049260 | MS.gene48796 | 0.861324 | 1.11E-63 | -1.69E-46 |
| MS.gene049346 | MS.gene48796 | 0.827544 | 1.48E-54 | -1.69E-46 |
| MS.gene049491 | MS.gene48796 | 0.834674 | 2.62E-56 | -1.69E-46 |
| MS.gene049599 | MS.gene48796 | -0.803509 | 3.37E-49 | -1.69E-46 |
| MS.gene049614 | MS.gene48796 | 0.811871 | 5.64E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene48796 | 0.881097 | 3.18E-70 | -1.69E-46 |
| MS.gene050076 | MS.gene48796 | 0.823951 | 1.05E-53 | -1.69E-46 |
| MS.gene050099 | MS.gene48796 | 0.846404 | 2.22E-59 | -1.69E-46 |
| MS.gene050128 | MS.gene48796 | 0.882918 | 6.96E-71 | -1.69E-46 |
| MS.gene050301 | MS.gene48796 | 0.870127 | 1.85E-66 | -1.69E-46 |
| MS.gene050397 | MS.gene48796 | 0.830547 | 2.76E-55 | -1.69E-46 |
| MS.gene050404 | MS.gene48796 | 0.831574 | 1.55E-55 | -1.69E-46 |
| MS.gene050406 | MS.gene48796 | 0.808715 | 2.70E-50 | -1.69E-46 |
| MS.gene050432 | MS.gene48796 | 0.817394 | 3.37E-52 | -1.69E-46 |
| MS.gene050529 | MS.gene48796 | 0.805378 | 1.37E-49 | -1.69E-46 |
| MS.gene050767 | MS.gene48796 | 0.805828 | 1.11E-49 | -1.69E-46 |
| MS.gene050770 | MS.gene48796 | 0.806636 | 7.47E-50 | -1.69E-46 |
| MS.gene05101 | MS.gene48796 | 0.855504 | 6.04E-62 | -1.69E-46 |
| MS.gene051471 | MS.gene48796 | 0.853578 | 2.18E-61 | -1.69E-46 |
| MS.gene051662 | MS.gene48796 | 0.806592 | 7.63E-50 | -1.69E-46 |
| MS.gene051663 | MS.gene48796 | 0.809543 | 1.80E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene48796 | 0.815735 | 7.94E-52 | -1.69E-46 |
| MS.gene051860 | MS.gene48796 | 0.802609 | 5.17E-49 | -1.69E-46 |
| MS.gene052078 | MS.gene48796 | 0.855191 | 7.44E-62 | -1.69E-46 |
| MS.gene052178 | MS.gene48796 | 0.91629 | 2.13E-85 | -1.69E-46 |
| MS.gene052188 | MS.gene48796 | 0.81462 | 1.40E-51 | -1.69E-46 |
| MS.gene052207 | MS.gene48796 | 0.803888 | 2.81E-49 | -1.69E-46 |
| MS.gene052287 | MS.gene48796 | 0.860124 | 2.57E-63 | -1.69E-46 |
| MS.gene052290 | MS.gene48796 | 0.808646 | 2.80E-50 | -1.69E-46 |
| MS.gene052310 | MS.gene48796 | 0.816528 | 5.28E-52 | -1.69E-46 |
| MS.gene052326 | MS.gene48796 | 0.824506 | 7.78E-54 | -1.69E-46 |
| MS.gene052340 | MS.gene48796 | 0.852491 | 4.46E-61 | -1.69E-46 |
| MS.gene053073 | MS.gene48796 | 0.830942 | 2.21E-55 | -1.69E-46 |
| MS.gene053209 | MS.gene48796 | 0.854856 | 9.31E-62 | -1.69E-46 |
| MS.gene053557 | MS.gene48796 | 0.878911 | 1.91E-69 | -1.69E-46 |
| MS.gene053836 | MS.gene48796 | 0.854158 | 1.48E-61 | -1.69E-46 |
| MS.gene053907 | MS.gene48796 | 0.80905 | 2.29E-50 | -1.69E-46 |
| MS.gene054010 | MS.gene48796 | 0.806104 | 9.67E-50 | -1.69E-46 |
| MS.gene054011 | MS.gene48796 | 0.825976 | 3.49E-54 | -1.69E-46 |
| MS.gene054012 | MS.gene48796 | 0.81989 | 9.16E-53 | -1.69E-46 |
| MS.gene054013 | MS.gene48796 | 0.818289 | 2.12E-52 | -1.69E-46 |
| MS.gene054184 | MS.gene48796 | 0.816154 | 6.40E-52 | -1.69E-46 |
| MS.gene054185 | MS.gene48796 | 0.819212 | 1.31E-52 | -1.69E-46 |
| MS.gene054214 | MS.gene48796 | 0.858395 | 8.48E-63 | -1.69E-46 |
| MS.gene054376 | MS.gene48796 | 0.814776 | 1.30E-51 | -1.69E-46 |
| MS.gene054473 | MS.gene48796 | 0.802559 | 5.30E-49 | -1.69E-46 |
| MS.gene054618 | MS.gene48796 | 0.818997 | 1.46E-52 | -1.69E-46 |
| MS.gene055116 | MS.gene48796 | 0.800701 | 1.27E-48 | -1.69E-46 |
| MS.gene055133 | MS.gene48796 | 0.838294 | 3.13E-57 | -1.69E-46 |
| MS.gene055207 | MS.gene48796 | 0.809766 | 1.61E-50 | -1.69E-46 |
| MS.gene055254 | MS.gene48796 | 0.842932 | 1.91E-58 | -1.69E-46 |
| MS.gene055277 | MS.gene48796 | 0.889461 | 2.37E-73 | -1.69E-46 |
| MS.gene055303 | MS.gene48796 | 0.803055 | 4.18E-49 | -1.69E-46 |
| MS.gene055390 | MS.gene48796 | 0.853317 | 2.59E-61 | -1.69E-46 |
| MS.gene055422 | MS.gene48796 | 0.867828 | 1.03E-65 | -1.69E-46 |
| MS.gene055428 | MS.gene48796 | 0.848946 | 4.43E-60 | -1.69E-46 |
| MS.gene055604 | MS.gene48796 | 0.856012 | 4.29E-62 | -1.69E-46 |
| MS.gene055712 | MS.gene48796 | 0.800019 | 1.75E-48 | -1.69E-46 |
| MS.gene056069 | MS.gene48796 | -0.822017 | 2.97E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene48796 | 0.836509 | 8.98E-57 | -1.69E-46 |
| MS.gene056314 | MS.gene48796 | 0.836745 | 7.82E-57 | -1.69E-46 |
| MS.gene056410 | MS.gene48796 | 0.809728 | 1.64E-50 | -1.69E-46 |
| MS.gene056609 | MS.gene48796 | 0.811598 | 6.46E-51 | -1.69E-46 |
| MS.gene056612 | MS.gene48796 | 0.858286 | 9.14E-63 | -1.69E-46 |
| MS.gene056772 | MS.gene48796 | 0.848422 | 6.19E-60 | -1.69E-46 |
| MS.gene056931 | MS.gene48796 | 0.804194 | 2.43E-49 | -1.69E-46 |
| MS.gene056933 | MS.gene48796 | 0.803959 | 2.72E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene48796 | 0.850485 | 1.65E-60 | -1.69E-46 |
| MS.gene057121 | MS.gene48796 | 0.80553 | 1.28E-49 | -1.69E-46 |
| MS.gene05733 | MS.gene48796 | 0.824026 | 1.01E-53 | -1.69E-46 |
| MS.gene057439 | MS.gene48796 | 0.840566 | 8.05E-58 | -1.69E-46 |
| MS.gene057448 | MS.gene48796 | 0.818154 | 2.27E-52 | -1.69E-46 |
| MS.gene057449 | MS.gene48796 | 0.811688 | 6.18E-51 | -1.69E-46 |
| MS.gene057450 | MS.gene48796 | 0.832113 | 1.14E-55 | -1.69E-46 |
| MS.gene057754 | MS.gene48796 | 0.813772 | 2.16E-51 | -1.69E-46 |
| MS.gene057794 | MS.gene48796 | 0.825149 | 5.49E-54 | -1.69E-46 |
| MS.gene057796 | MS.gene48796 | 0.836365 | 9.77E-57 | -1.69E-46 |
| MS.gene058002 | MS.gene48796 | 0.851673 | 7.61E-61 | -1.69E-46 |
| MS.gene058082 | MS.gene48796 | 0.830374 | 3.04E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene48796 | 0.862692 | 4.23E-64 | -1.69E-46 |
| MS.gene058142 | MS.gene48796 | 0.811531 | 6.68E-51 | -1.69E-46 |
| MS.gene058236 | MS.gene48796 | 0.806386 | 8.44E-50 | -1.69E-46 |
| MS.gene058276 | MS.gene48796 | 0.804828 | 1.79E-49 | -1.69E-46 |
| MS.gene058293 | MS.gene48796 | 0.806267 | 8.94E-50 | -1.69E-46 |
| MS.gene058365 | MS.gene48796 | 0.801916 | 7.18E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene48796 | 0.827973 | 1.16E-54 | -1.69E-46 |
| MS.gene058845 | MS.gene48796 | 0.818926 | 1.52E-52 | -1.69E-46 |
| MS.gene05907 | MS.gene48796 | 0.826658 | 2.40E-54 | -1.69E-46 |
| MS.gene059228 | MS.gene48796 | 0.870731 | 1.17E-66 | -1.69E-46 |
| MS.gene059294 | MS.gene48796 | 0.834437 | 3.00E-56 | -1.69E-46 |
| MS.gene059326 | MS.gene48796 | 0.804635 | 1.97E-49 | -1.69E-46 |
| MS.gene059518 | MS.gene48796 | 0.865803 | 4.53E-65 | -1.69E-46 |
| MS.gene059597 | MS.gene48796 | 0.822915 | 1.83E-53 | -1.69E-46 |
| MS.gene059688 | MS.gene48796 | 0.819393 | 1.19E-52 | -1.69E-46 |
| MS.gene059692 | MS.gene48796 | 0.846755 | 1.78E-59 | -1.69E-46 |
| MS.gene059695 | MS.gene48796 | 0.839892 | 1.21E-57 | -1.69E-46 |
| MS.gene059708 | MS.gene48796 | 0.820916 | 5.33E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene48796 | 0.847795 | 9.22E-60 | -1.69E-46 |
| MS.gene059894 | MS.gene48796 | 0.899428 | 2.00E-77 | -1.69E-46 |
| MS.gene059899 | MS.gene48796 | 0.800902 | 1.16E-48 | -1.69E-46 |
| MS.gene059974 | MS.gene48796 | 0.880362 | 5.85E-70 | -1.69E-46 |
| MS.gene060005 | MS.gene48796 | 0.854073 | 1.57E-61 | -1.69E-46 |
| MS.gene06037 | MS.gene48796 | 0.878646 | 2.37E-69 | -1.69E-46 |
| MS.gene060407 | MS.gene48796 | 0.806476 | 8.07E-50 | -1.69E-46 |
| MS.gene060476 | MS.gene48796 | 0.876854 | 1.00E-68 | -1.69E-46 |
| MS.gene060559 | MS.gene48796 | 0.80551 | 1.29E-49 | -1.69E-46 |
| MS.gene060800 | MS.gene48796 | 0.807864 | 4.10E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene48796 | 0.894821 | 1.72E-75 | -1.69E-46 |
| MS.gene061051 | MS.gene48796 | -0.803267 | 3.78E-49 | -1.69E-46 |
| MS.gene061112 | MS.gene48796 | 0.862168 | 6.13E-64 | -1.69E-46 |
| MS.gene061113 | MS.gene48796 | 0.814584 | 1.43E-51 | -1.69E-46 |
| MS.gene061265 | MS.gene48796 | 0.854186 | 1.46E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene76710 | MS.gene48796 | PPI |
| MS.gene48796 | MS.gene82219 | PPI |
| MS.gene48796 | MS.gene76747 | PPI |
| MS.gene48796 | MS.gene48797 | PPI |
| MS.gene76747 | MS.gene48796 | PPI |
| MS.gene48831 | MS.gene48796 | PPI |
| MS.gene53305 | MS.gene48796 | PPI |
| MS.gene48796 | MS.gene82253 | PPI |
| MS.gene48796 | MS.gene48829 | PPI |
| MS.gene82256 | MS.gene48796 | PPI |
| MS.gene82221 | MS.gene48796 | PPI |
| MS.gene48796 | MS.gene76745 | PPI |
| MS.gene48796 | MS.gene82222 | PPI |
| MS.gene53311 | MS.gene48796 | PPI |
| MS.gene76713 | MS.gene48796 | PPI |
| MS.gene48796 | MS.gene53309 | PPI |
| MS.gene77025 | MS.gene48796 | PPI |
| MS.gene48835 | MS.gene48796 | PPI |
| MS.gene48796 | MS.gene76714 | PPI |
| MS.gene82253 | MS.gene48796 | PPI |
| MS.gene77022 | MS.gene48796 | PPI |
| MS.gene48828 | MS.gene48796 | PPI |
| MS.gene76705 | MS.gene48796 | PPI |
| MS.gene53312 | MS.gene48796 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48796.t1 | MTR_4g029390 | 91.525 | 118 | 9 | 1 | 1 | 118 | 1 | 117 | 9.45e-73 | 212 |
| MS.gene48796.t1 | MTR_4g029350 | 82.203 | 118 | 20 | 1 | 1 | 118 | 1 | 117 | 1.50e-65 | 194 |
| MS.gene48796.t1 | MTR_4g428370 | 70.940 | 117 | 32 | 1 | 1 | 117 | 58 | 172 | 1.78e-51 | 160 |
| MS.gene48796.t1 | MTR_4g027840 | 66.667 | 117 | 37 | 1 | 1 | 117 | 1 | 115 | 1.22e-48 | 152 |
| MS.gene48796.t1 | MTR_4g027800 | 65.812 | 117 | 38 | 1 | 1 | 117 | 1 | 115 | 1.52e-48 | 151 |
| MS.gene48796.t1 | MTR_4g029210 | 50.442 | 113 | 54 | 2 | 1 | 113 | 1 | 111 | 1.10e-28 | 100 |
| MS.gene48796.t1 | MTR_4g028180 | 48.246 | 114 | 51 | 4 | 11 | 117 | 14 | 126 | 1.94e-25 | 93.2 |
| MS.gene48796.t1 | MTR_2g062600 | 40.171 | 117 | 66 | 3 | 1 | 116 | 1 | 114 | 4.88e-24 | 89.0 |
| MS.gene48796.t1 | MTR_7g072930 | 43.103 | 116 | 63 | 2 | 2 | 115 | 3 | 117 | 6.12e-24 | 89.7 |
| MS.gene48796.t1 | MTR_7g073170 | 40.367 | 109 | 62 | 3 | 11 | 118 | 13 | 119 | 4.80e-22 | 84.3 |
| MS.gene48796.t1 | MTR_7g072830 | 34.454 | 119 | 72 | 2 | 4 | 116 | 1 | 119 | 5.55e-22 | 84.0 |
| MS.gene48796.t1 | MTR_3g467100 | 35.398 | 113 | 72 | 1 | 5 | 117 | 3 | 114 | 2.34e-21 | 82.4 |
| MS.gene48796.t1 | MTR_7g073150 | 41.379 | 116 | 65 | 2 | 2 | 115 | 3 | 117 | 2.57e-21 | 82.8 |
| MS.gene48796.t1 | MTR_4g028190 | 36.975 | 119 | 73 | 2 | 1 | 118 | 5 | 122 | 8.11e-21 | 81.3 |
| MS.gene48796.t1 | MTR_4g028250 | 37.288 | 118 | 73 | 1 | 1 | 117 | 1 | 118 | 2.87e-20 | 79.7 |
| MS.gene48796.t1 | MTR_7g072730 | 34.483 | 116 | 74 | 1 | 2 | 115 | 3 | 118 | 3.36e-20 | 80.9 |
| MS.gene48796.t1 | MTR_7g072993 | 37.069 | 116 | 70 | 1 | 2 | 114 | 3 | 118 | 8.69e-17 | 71.2 |
| MS.gene48796.t1 | MTR_7g073120 | 34.483 | 116 | 73 | 2 | 2 | 114 | 3 | 118 | 1.23e-16 | 71.2 |
| MS.gene48796.t1 | MTR_4g028310 | 32.500 | 120 | 76 | 3 | 1 | 117 | 1 | 118 | 1.04e-15 | 68.6 |
| MS.gene48796.t1 | MTR_7g072980 | 32.174 | 115 | 76 | 1 | 2 | 114 | 3 | 117 | 1.81e-15 | 67.8 |
| MS.gene48796.t1 | MTR_3g056640 | 31.967 | 122 | 77 | 4 | 1 | 117 | 2 | 122 | 2.93e-15 | 67.0 |
| MS.gene48796.t1 | MTR_7g073130 | 34.211 | 114 | 73 | 2 | 2 | 114 | 3 | 115 | 4.08e-15 | 66.6 |
| MS.gene48796.t1 | MTR_7g072900 | 35.088 | 114 | 73 | 1 | 2 | 114 | 3 | 116 | 8.16e-15 | 65.5 |
| MS.gene48796.t1 | MTR_1g040335 | 35.897 | 117 | 72 | 2 | 2 | 116 | 3 | 118 | 1.51e-14 | 65.1 |
| MS.gene48796.t1 | MTR_7g451500 | 33.981 | 103 | 62 | 2 | 15 | 116 | 1 | 98 | 1.59e-14 | 64.3 |
| MS.gene48796.t1 | MTR_3g046530 | 29.060 | 117 | 79 | 3 | 4 | 117 | 7 | 122 | 4.01e-14 | 63.9 |
| MS.gene48796.t1 | MTR_3g046350 | 32.143 | 112 | 68 | 5 | 8 | 115 | 10 | 117 | 4.20e-14 | 63.9 |
| MS.gene48796.t1 | MTR_7g072960 | 37.391 | 115 | 55 | 3 | 3 | 115 | 4 | 103 | 4.27e-14 | 64.3 |
| MS.gene48796.t1 | MTR_3g046515 | 29.565 | 115 | 77 | 3 | 4 | 115 | 7 | 120 | 7.14e-14 | 63.2 |
| MS.gene48796.t1 | MTR_3g046540 | 29.060 | 117 | 79 | 3 | 4 | 117 | 7 | 122 | 1.04e-13 | 62.8 |
| MS.gene48796.t1 | MTR_3g464130 | 35.556 | 90 | 55 | 2 | 27 | 114 | 18 | 106 | 2.68e-13 | 61.6 |
| MS.gene48796.t1 | MTR_7g072810 | 36.449 | 107 | 59 | 3 | 11 | 115 | 13 | 112 | 1.01e-12 | 60.5 |
| MS.gene48796.t1 | MTR_3g464110 | 37.805 | 82 | 48 | 2 | 36 | 115 | 93 | 173 | 1.56e-12 | 60.8 |
| MS.gene48796.t1 | MTR_7g073060 | 32.759 | 116 | 77 | 1 | 2 | 116 | 3 | 118 | 3.48e-12 | 58.9 |
| MS.gene48796.t1 | MTR_4g028360 | 32.174 | 115 | 73 | 4 | 4 | 116 | 1 | 112 | 4.12e-11 | 56.2 |
| MS.gene48796.t1 | MTR_7g073100 | 30.702 | 114 | 78 | 1 | 2 | 114 | 3 | 116 | 8.99e-11 | 55.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene48796.t1 | AT5G59320 | 46.364 | 110 | 57 | 1 | 8 | 117 | 8 | 115 | 2.07e-28 | 100 |
| MS.gene48796.t1 | AT5G59310 | 45.455 | 110 | 55 | 2 | 8 | 117 | 8 | 112 | 6.74e-27 | 96.3 |
| MS.gene48796.t1 | AT2G38540 | 49.074 | 108 | 52 | 3 | 12 | 117 | 12 | 118 | 6.02e-25 | 91.7 |
| MS.gene48796.t1 | AT3G51590 | 43.478 | 115 | 61 | 2 | 6 | 116 | 1 | 115 | 7.53e-25 | 91.3 |
| MS.gene48796.t1 | AT2G38530 | 47.664 | 107 | 55 | 1 | 12 | 117 | 12 | 118 | 3.47e-24 | 89.7 |
| MS.gene48796.t1 | AT3G51600 | 48.148 | 108 | 53 | 3 | 12 | 117 | 12 | 118 | 4.56e-24 | 89.4 |
| MS.gene48796.t1 | AT5G01870 | 44.545 | 110 | 55 | 4 | 13 | 117 | 8 | 116 | 1.70e-23 | 87.8 |
| MS.gene48796.t1 | AT4G33355 | 42.593 | 108 | 58 | 1 | 13 | 116 | 11 | 118 | 6.44e-23 | 86.7 |
| MS.gene48796.t1 | AT4G33355 | 41.509 | 106 | 58 | 1 | 13 | 114 | 11 | 116 | 1.13e-21 | 83.2 |
| MS.gene48796.t1 | AT3G08770 | 44.330 | 97 | 50 | 3 | 24 | 117 | 18 | 113 | 1.30e-20 | 80.5 |
| MS.gene48796.t1 | AT3G08770 | 44.211 | 95 | 49 | 3 | 24 | 115 | 18 | 111 | 1.44e-19 | 77.8 |
| MS.gene48796.t1 | AT2G15050 | 38.393 | 112 | 63 | 4 | 12 | 117 | 11 | 122 | 6.14e-19 | 76.3 |
| MS.gene48796.t1 | AT2G15050 | 38.182 | 110 | 62 | 4 | 12 | 115 | 11 | 120 | 1.52e-17 | 72.8 |
| MS.gene48796.t1 | AT2G15050 | 38.835 | 103 | 59 | 3 | 12 | 110 | 11 | 113 | 5.08e-17 | 71.2 |
| MS.gene48796.t1 | AT2G18370 | 35.789 | 95 | 59 | 2 | 23 | 116 | 22 | 115 | 8.75e-16 | 68.2 |
Find 36 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACTTCTACTAACTGTAAT+AGG | 0.249879 | 4.4:+73515000 | MS.gene48796:CDS |
| CTTGCGGGCGGGGCCGTTGT+TGG | 0.344393 | 4.4:-73514868 | None:intergenic |
| GCAGTTGCAAGCAGTCTTGC+GGG | 0.361401 | 4.4:-73514883 | None:intergenic |
| TGCAAGCAGTCTTGCGGGCG+GGG | 0.373033 | 4.4:-73514878 | None:intergenic |
| CCATGCATTACTTATCTTCA+AGG | 0.382464 | 4.4:+73514789 | MS.gene48796:CDS |
| AGTAGAAGTGCTAAACTTGT+AGG | 0.409303 | 4.4:-73514988 | None:intergenic |
| TTGCAAGCAGTCTTGCGGGC+GGG | 0.409587 | 4.4:-73514879 | None:intergenic |
| ACTTATCTTCAAGGTGGTGC+TGG | 0.410647 | 4.4:+73514798 | MS.gene48796:CDS |
| GTGACTGCTGCCTCTGCCAT+TGG | 0.435322 | 4.4:-73514738 | None:intergenic |
| GATAAGTAATGCATGGAGTA+AGG | 0.447792 | 4.4:-73514782 | None:intergenic |
| GCGGGCGGGGCCGTTGTTGG+CGG | 0.458612 | 4.4:-73514865 | None:intergenic |
| AACTGCTTGAAAAGTGCTGC+TGG | 0.487551 | 4.4:+73514900 | MS.gene48796:CDS |
| AAGCATGAGAGTTGCATTTG+TGG | 0.488751 | 4.4:+73514683 | MS.gene48796:CDS |
| ATGTTAACACCACATTTGCC+AGG | 0.490963 | 4.4:-73514963 | None:intergenic |
| GCTGCTCTCCCTGGCAAATG+TGG | 0.495611 | 4.4:+73514954 | MS.gene48796:CDS |
| AGCAGTTGCAAGCAGTCTTG+CGG | 0.506600 | 4.4:-73514884 | None:intergenic |
| GTTGTTATTCAAACGAGCAA+TGG | 0.508618 | 4.4:-73514925 | None:intergenic |
| AACCAAGCTGCTGCTCTCCC+TGG | 0.514408 | 4.4:+73514945 | MS.gene48796:CDS |
| GGTGGAGTGAGGAGACTTAA+CGG | 0.519956 | 4.4:+73514840 | MS.gene48796:CDS |
| TGCCAGGGAGAGCAGCAGCT+TGG | 0.525683 | 4.4:-73514947 | None:intergenic |
| GGTCCATCTGCAGCATGCTG+TGG | 0.539612 | 4.4:+73514819 | MS.gene48796:CDS |
| GTTGCAAGCAGTCTTGCGGG+CGG | 0.541246 | 4.4:-73514880 | None:intergenic |
| CCATCTGCAGCATGCTGTGG+TGG | 0.550747 | 4.4:+73514822 | MS.gene48796:CDS |
| GTAGAAGTGCTAAACTTGTA+GGG | 0.551668 | 4.4:-73514987 | None:intergenic |
| GTCACATGTGGACAAGTAGT+AGG | 0.553840 | 4.4:+73514756 | MS.gene48796:CDS |
| GGTTAGTGCACCAATGGCAG+AGG | 0.566893 | 4.4:+73514728 | MS.gene48796:CDS |
| CCACCACAGCATGCTGCAGA+TGG | 0.580113 | 4.4:-73514822 | None:intergenic |
| TGCAATTATGTGCATGATGG+TGG | 0.600070 | 4.4:+73514707 | MS.gene48796:CDS |
| CTTAACGGTGCCGCCAACAA+CGG | 0.605014 | 4.4:+73514855 | MS.gene48796:CDS |
| CCTTGAAGATAAGTAATGCA+TGG | 0.607580 | 4.4:-73514789 | None:intergenic |
| GATGGTGGTTAGTGCACCAA+TGG | 0.623706 | 4.4:+73514722 | MS.gene48796:CDS |
| GGCTGCAATTATGTGCATGA+TGG | 0.625253 | 4.4:+73514704 | MS.gene48796:CDS |
| GCAGAGGCAGCAGTCACATG+TGG | 0.630368 | 4.4:+73514744 | MS.gene48796:CDS |
| TGTTAACACCACATTTGCCA+GGG | 0.642773 | 4.4:-73514962 | None:intergenic |
| CAGCATGCTGTGGTGGAGTG+AGG | 0.648040 | 4.4:+73514829 | MS.gene48796:CDS |
| TGCATTACTTATCTTCAAGG+TGG | 0.657751 | 4.4:+73514792 | MS.gene48796:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATATATATATATATAT+AGG | - | chr4.4:73515255-73515236 | None:intergenic | 0.0% |
| !! | TATATATATATATATATATA+GGG | - | chr4.4:73515254-73515235 | None:intergenic | 0.0% |
| !! | ATTTCTCATATACGTAATTA+AGG | - | chr4.4:73515044-73515063 | None:intergenic | 20.0% |
| !! | GGCAAATTTAATAAGTTATT+CGG | - | chr4.4:73515409-73515428 | None:intergenic | 20.0% |
| ! | ATTTGCCTAAAAAAATGAAG+TGG | + | chr4.4:73515422-73515441 | MS.gene48796:intron | 25.0% |
| ! | TGAGACATTGAAATGATAAT+TGG | + | chr4.4:73515565-73515584 | MS.gene48796:intron | 25.0% |
| !! | TCTCTAAGAATACTCTTTAA+CGG | + | chr4.4:73515074-73515093 | MS.gene48796:intron | 25.0% |
| !!! | AATCTTTAAAGAGTACCATT+AGG | + | chr4.4:73515199-73515218 | MS.gene48796:intron | 25.0% |
| !!! | ATCTTTAAAGAGTACCATTA+GGG | + | chr4.4:73515200-73515219 | MS.gene48796:intron | 25.0% |
| !!! | TTGTTCCACTTCATTTTTTT+AGG | - | chr4.4:73515430-73515449 | None:intergenic | 25.0% |
| AACTATCTATATGCTCATGA+AGG | - | chr4.4:73515792-73515811 | None:intergenic | 30.0% | |
| ACTATCTATATGCTCATGAA+GGG | - | chr4.4:73515791-73515810 | None:intergenic | 30.0% | |
| ATTGAAATGATAATTGGAGG+AGG | + | chr4.4:73515571-73515590 | MS.gene48796:intron | 30.0% | |
| GACATTGAAATGATAATTGG+AGG | + | chr4.4:73515568-73515587 | MS.gene48796:intron | 30.0% | |
| ! | ATGAGTGACTTATGCATTTT+TGG | + | chr4.4:73515660-73515679 | MS.gene48796:intron | 30.0% |
| ! | TTTTTGGACAGTCATCAAAA+TGG | + | chr4.4:73515676-73515695 | MS.gene48796:intron | 30.0% |
| !! | TATTTTTGTTCCACTTCTTC+AGG | - | chr4.4:73515386-73515405 | None:intergenic | 30.0% |
| CCATGCATTACTTATCTTCA+AGG | + | chr4.4:73514789-73514808 | MS.gene48796:CDS | 35.0% | |
| CCTTGAAGATAAGTAATGCA+TGG | - | chr4.4:73514792-73514811 | None:intergenic | 35.0% | |
| GATAAGTAATGCATGGAGTA+AGG | - | chr4.4:73514785-73514804 | None:intergenic | 35.0% | |
| GCACTTCTACTAACTGTAAT+AGG | + | chr4.4:73515000-73515019 | MS.gene48796:CDS | 35.0% | |
| TGCATTACTTATCTTCAAGG+TGG | + | chr4.4:73514792-73514811 | MS.gene48796:CDS | 35.0% | |
| ! | GTCTCACTCTCACTTAATTT+TGG | - | chr4.4:73515551-73515570 | None:intergenic | 35.0% |
| ! | GTTGTTATTCAAACGAGCAA+TGG | - | chr4.4:73514928-73514947 | None:intergenic | 35.0% |
| !! | AGTAGAAGTGCTAAACTTGT+AGG | - | chr4.4:73514991-73515010 | None:intergenic | 35.0% |
| !! | GTAGAAGTGCTAAACTTGTA+GGG | - | chr4.4:73514990-73515009 | None:intergenic | 35.0% |
| !!! | TCATCATCTTTTTAGCTTGG+AGG | - | chr4.4:73515528-73515547 | None:intergenic | 35.0% |
| !!! | TGGTCATCATCTTTTTAGCT+TGG | - | chr4.4:73515531-73515550 | None:intergenic | 35.0% |
| AAGCATGAGAGTTGCATTTG+TGG | + | chr4.4:73514683-73514702 | MS.gene48796:CDS | 40.0% | |
| ATCTCAAGTACCTGAAGAAG+TGG | + | chr4.4:73515373-73515392 | MS.gene48796:intron | 40.0% | |
| ATGTTAACACCACATTTGCC+AGG | - | chr4.4:73514966-73514985 | None:intergenic | 40.0% | |
| TGCAATTATGTGCATGATGG+TGG | + | chr4.4:73514707-73514726 | MS.gene48796:CDS | 40.0% | |
| TGTTAACACCACATTTGCCA+GGG | - | chr4.4:73514965-73514984 | None:intergenic | 40.0% | |
| ! | CTTTAACGGTCTAAAGTAGC+AGG | + | chr4.4:73515088-73515107 | MS.gene48796:intron | 40.0% |
| ! | GTTTTCTGTATTGCAGCATC+AGG | + | chr4.4:73515869-73515888 | MS.gene48796:intron | 40.0% |
| !! | TAGGGTTTTGTGTTCCCTAA+TGG | - | chr4.4:73515217-73515236 | None:intergenic | 40.0% |
| ACTTATCTTCAAGGTGGTGC+TGG | + | chr4.4:73514798-73514817 | MS.gene48796:CDS | 45.0% | |
| GGCTGCAATTATGTGCATGA+TGG | + | chr4.4:73514704-73514723 | MS.gene48796:CDS | 45.0% | |
| GTCACATGTGGACAAGTAGT+AGG | + | chr4.4:73514756-73514775 | MS.gene48796:CDS | 45.0% | |
| !! | AACTGCTTGAAAAGTGCTGC+TGG | + | chr4.4:73514900-73514919 | MS.gene48796:CDS | 45.0% |
| !!! | ACAAATTTTTTTTTTTAAAA+TGG | + | chr4.4:73515446-73515465 | MS.gene48796:intron | 5.0% |
| AGCAGTTGCAAGCAGTCTTG+CGG | - | chr4.4:73514887-73514906 | None:intergenic | 50.0% | |
| GGTGGAGTGAGGAGACTTAA+CGG | + | chr4.4:73514840-73514859 | MS.gene48796:CDS | 50.0% | |
| ! | GATGGTGGTTAGTGCACCAA+TGG | + | chr4.4:73514722-73514741 | MS.gene48796:CDS | 50.0% |
| GCAGTTGCAAGCAGTCTTGC+GGG | - | chr4.4:73514886-73514905 | None:intergenic | 55.0% | |
| GGTTAGTGCACCAATGGCAG+AGG | + | chr4.4:73514728-73514747 | MS.gene48796:CDS | 55.0% | |
| !! | CTTAACGGTGCCGCCAACAA+CGG | + | chr4.4:73514855-73514874 | MS.gene48796:CDS | 55.0% |
| AACCAAGCTGCTGCTCTCCC+TGG | + | chr4.4:73514945-73514964 | MS.gene48796:CDS | 60.0% | |
| CCACCACAGCATGCTGCAGA+TGG | - | chr4.4:73514825-73514844 | None:intergenic | 60.0% | |
| CCATCTGCAGCATGCTGTGG+TGG | + | chr4.4:73514822-73514841 | MS.gene48796:CDS | 60.0% | |
| GCAGAGGCAGCAGTCACATG+TGG | + | chr4.4:73514744-73514763 | MS.gene48796:CDS | 60.0% | |
| GCTGCTCTCCCTGGCAAATG+TGG | + | chr4.4:73514954-73514973 | MS.gene48796:CDS | 60.0% | |
| GGTCCATCTGCAGCATGCTG+TGG | + | chr4.4:73514819-73514838 | MS.gene48796:CDS | 60.0% | |
| GTGACTGCTGCCTCTGCCAT+TGG | - | chr4.4:73514741-73514760 | None:intergenic | 60.0% | |
| GTTGCAAGCAGTCTTGCGGG+CGG | - | chr4.4:73514883-73514902 | None:intergenic | 60.0% | |
| TTGCAAGCAGTCTTGCGGGC+GGG | - | chr4.4:73514882-73514901 | None:intergenic | 60.0% | |
| ! | CAGCATGCTGTGGTGGAGTG+AGG | + | chr4.4:73514829-73514848 | MS.gene48796:CDS | 60.0% |
| TGCAAGCAGTCTTGCGGGCG+GGG | - | chr4.4:73514881-73514900 | None:intergenic | 65.0% | |
| TGCCAGGGAGAGCAGCAGCT+TGG | - | chr4.4:73514950-73514969 | None:intergenic | 65.0% | |
| CTTGCGGGCGGGGCCGTTGT+TGG | - | chr4.4:73514871-73514890 | None:intergenic | 75.0% | |
| ! | GCGGGCGGGGCCGTTGTTGG+CGG | - | chr4.4:73514868-73514887 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 73514678 | 73515897 | 73514678 | ID=MS.gene48796 |
| chr4.4 | mRNA | 73514678 | 73515897 | 73514678 | ID=MS.gene48796.t1;Parent=MS.gene48796 |
| chr4.4 | exon | 73514678 | 73515021 | 73514678 | ID=MS.gene48796.t1.exon1;Parent=MS.gene48796.t1 |
| chr4.4 | CDS | 73514678 | 73515021 | 73514678 | ID=cds.MS.gene48796.t1;Parent=MS.gene48796.t1 |
| chr4.4 | exon | 73515885 | 73515897 | 73515885 | ID=MS.gene48796.t1.exon2;Parent=MS.gene48796.t1 |
| chr4.4 | CDS | 73515885 | 73515897 | 73515885 | ID=cds.MS.gene48796.t1;Parent=MS.gene48796.t1 |
| Gene Sequence |
| Protein sequence |
