AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48797


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48797.t1 MTR_4g029350 93.162 117 8 0 1 117 1 117 7.91e-75 217
MS.gene48797.t1 MTR_4g029390 83.761 117 19 0 1 117 1 117 3.27e-66 196
MS.gene48797.t1 MTR_4g428370 64.103 117 39 2 1 116 58 172 1.86e-44 142
MS.gene48797.t1 MTR_4g027840 61.538 117 42 2 1 116 1 115 1.48e-43 139
MS.gene48797.t1 MTR_4g027800 60.684 117 43 2 1 116 1 115 2.31e-43 138
MS.gene48797.t1 MTR_4g029210 50.000 112 55 1 1 112 1 111 2.80e-31 107
MS.gene48797.t1 MTR_4g028180 45.299 117 55 4 8 116 11 126 1.45e-25 93.2
MS.gene48797.t1 MTR_4g028190 39.831 118 70 1 1 117 5 122 2.53e-24 90.1
MS.gene48797.t1 MTR_7g072930 40.187 107 61 2 10 114 12 117 2.54e-21 83.2
MS.gene48797.t1 MTR_3g467100 34.821 112 73 0 5 116 3 114 5.57e-20 78.6
MS.gene48797.t1 MTR_7g072830 35.043 117 69 3 7 116 4 120 3.73e-19 76.6
MS.gene48797.t1 MTR_7g073150 39.655 116 66 3 2 114 3 117 5.57e-19 76.6
MS.gene48797.t1 MTR_2g062600 34.906 106 67 2 11 115 10 114 4.10e-18 73.9
MS.gene48797.t1 MTR_7g073170 36.937 111 64 5 10 117 12 119 9.17e-18 73.2
MS.gene48797.t1 MTR_3g056640 33.621 116 74 2 3 116 8 122 1.40e-17 72.8
MS.gene48797.t1 MTR_7g072730 34.188 117 72 4 2 114 3 118 3.48e-17 72.8
MS.gene48797.t1 MTR_3g046515 31.579 114 75 2 3 114 8 120 3.09e-16 69.3
MS.gene48797.t1 MTR_4g028250 33.898 118 76 2 1 116 1 118 4.44e-16 68.9
MS.gene48797.t1 MTR_3g046350 34.513 113 68 3 4 114 9 117 5.09e-16 68.6
MS.gene48797.t1 MTR_3g046530 31.034 116 77 2 3 116 8 122 6.97e-16 68.6
MS.gene48797.t1 MTR_4g028310 35.833 120 71 3 1 116 1 118 7.86e-16 68.6
MS.gene48797.t1 MTR_3g046540 31.034 116 77 2 3 116 8 122 1.13e-15 67.8
MS.gene48797.t1 MTR_3g464130 34.951 103 64 2 13 113 5 106 4.24e-15 65.9
MS.gene48797.t1 MTR_4g028360 32.456 114 73 3 4 115 1 112 2.35e-14 64.7
MS.gene48797.t1 MTR_7g451500 33.684 95 62 1 22 115 4 98 2.67e-14 63.9
MS.gene48797.t1 MTR_3g464110 40.964 83 46 2 34 114 92 173 5.52e-14 64.7
MS.gene48797.t1 MTR_7g073120 33.898 118 73 3 1 113 1 118 6.43e-14 64.3
MS.gene48797.t1 MTR_7g072993 35.345 116 71 2 2 113 3 118 2.11e-13 62.4
MS.gene48797.t1 MTR_7g073130 32.456 114 74 3 2 113 3 115 8.86e-13 60.5
MS.gene48797.t1 MTR_7g072900 38.053 113 64 5 4 113 7 116 3.71e-12 58.9
MS.gene48797.t1 MTR_1g040335 38.333 120 67 5 1 115 1 118 4.71e-12 58.5
MS.gene48797.t1 MTR_7g072960 34.783 115 53 4 4 114 7 103 5.75e-12 58.5
MS.gene48797.t1 MTR_7g072980 31.304 115 76 2 2 113 3 117 2.28e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48797.t1 AT5G59320 50.000 110 52 1 7 116 9 115 4.73e-29 102
MS.gene48797.t1 AT5G59310 52.475 101 45 1 16 116 15 112 1.98e-27 97.8
MS.gene48797.t1 AT3G51590 45.872 109 53 3 12 115 8 115 1.31e-24 90.9
MS.gene48797.t1 AT2G38540 44.538 119 62 4 1 116 1 118 2.13e-23 87.8
MS.gene48797.t1 AT4G33355 44.348 115 57 1 1 115 11 118 5.77e-23 86.7
MS.gene48797.t1 AT4G33355 43.363 113 57 1 1 113 11 116 9.23e-22 83.6
MS.gene48797.t1 AT5G01870 39.655 116 66 3 4 116 2 116 1.08e-21 83.2
MS.gene48797.t1 AT3G51600 40.678 118 68 2 1 116 1 118 3.38e-21 82.0
MS.gene48797.t1 AT2G15050 40.179 112 60 4 12 116 11 122 9.86e-21 80.9
MS.gene48797.t1 AT2G38530 44.348 115 57 3 9 116 4 118 1.31e-20 80.5
MS.gene48797.t1 AT2G15050 40.909 110 58 4 12 114 11 120 8.95e-20 78.6
MS.gene48797.t1 AT2G15050 41.748 103 55 3 12 109 11 113 2.83e-19 77.0
MS.gene48797.t1 AT3G08770 43.158 95 50 3 25 116 20 113 7.29e-19 75.9
MS.gene48797.t1 AT3G08770 43.011 93 49 3 25 114 20 111 8.11e-18 73.2
MS.gene48797.t1 AT2G18370 31.034 116 78 2 1 115 1 115 6.58e-15 65.9

Find 34 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAGAAGTGCTAAACTTAT+AGG 0.238471 4.4:-73535129 None:intergenic
GCACTTCTACCAACTGTAAT+AGG 0.262122 4.4:+73535141 MS.gene48797:CDS
GATAAGTAATGCATGGAATA+AGG 0.317466 4.4:-73534923 None:intergenic
CATGTGGCTGCCTCTGCTAT+TGG 0.339290 4.4:-73534882 None:intergenic
CCATCTCCACAGTGCTGTGC+TGG 0.355566 4.4:+73534963 MS.gene48797:CDS
CCATGCATTACTTATCTTCA+AGG 0.366651 4.4:+73534930 MS.gene48797:CDS
TTGCAAGCAGCCTTGCGAGC+CGG 0.406399 4.4:-73535020 None:intergenic
CAACACTGGCCCGGCTCGCA+AGG 0.417244 4.4:+73535010 MS.gene48797:CDS
CACAGTGCTGTGCTGGAGTA+AGG 0.420837 4.4:+73534970 MS.gene48797:CDS
TGCAAGCAGCCTTGCGAGCC+GGG 0.430405 4.4:-73535019 None:intergenic
GTAGAAGTGCTAAACTTATA+GGG 0.434237 4.4:-73535128 None:intergenic
TGCAATTATGTGCATGGTGG+TGG 0.439721 4.4:+73534851 MS.gene48797:CDS
CTTGCGAGCCGGGCCAGTGT+TGG 0.451576 4.4:-73535009 None:intergenic
CGCCGCCGCCAACACTGGCC+CGG 0.466490 4.4:+73535001 MS.gene48797:CDS
AGCCGGGCCAGTGTTGGCGG+CGG 0.471641 4.4:-73535003 None:intergenic
ACTTATCTTCAAGGAGGTGC+TGG 0.481218 4.4:+73534939 MS.gene48797:CDS
GCGAGCCGGGCCAGTGTTGG+CGG 0.487316 4.4:-73535006 None:intergenic
GCTGCTCTCCCTGGCAAATG+TGG 0.495611 4.4:+73535095 MS.gene48797:CDS
CTTAACGCCGCCGCCAACAC+TGG 0.495662 4.4:+73534996 MS.gene48797:CDS
CCAGCACAGCACTGTGGAGA+TGG 0.501400 4.4:-73534963 None:intergenic
AACCAAGCTGCTGCTCTCCC+TGG 0.514408 4.4:+73535086 MS.gene48797:CDS
TGCCAGGGAGAGCAGCAGCT+TGG 0.525683 4.4:-73535088 None:intergenic
GTTGTTATTCAAACGAGCAA+TGG 0.551011 4.4:-73535066 None:intergenic
AACTGCTTGAAAAGTGCTGC+TGG 0.566956 4.4:+73535041 MS.gene48797:CDS
ATCAACTAGTTGACCGCATG+TGG 0.580514 4.4:-73534898 None:intergenic
CCTTGAAGATAAGTAATGCA+TGG 0.589008 4.4:-73534930 None:intergenic
ACAAACATACCTATTACAGT+TGG 0.595621 4.4:-73535150 None:intergenic
TGTGGTTGCAATTATGTGCA+TGG 0.608819 4.4:+73534845 MS.gene48797:CDS
GGTTAGTGCACCAATAGCAG+AGG 0.668844 4.4:+73534872 MS.gene48797:CDS
TGCATTACTTATCTTCAAGG+AGG 0.675764 4.4:+73534933 MS.gene48797:CDS
AAGCTTGAGAGTTACAATTG+TGG 0.688534 4.4:+73534827 MS.gene48797:CDS
CTTACTCCAGCACAGCACTG+TGG 0.718135 4.4:-73534969 None:intergenic
GGTTGCAATTATGTGCATGG+TGG 0.720687 4.4:+73534848 MS.gene48797:CDS
ATAGCAGAGGCAGCCACATG+CGG 0.724717 4.4:+73534885 MS.gene48797:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGTCATTTATTTATTTTTTT+TGG + chr4.4:73535446-73535465 MS.gene48797:intron 10.0%
!! AATTAGGTATTATTATAACA+GGG + chr4.4:73535495-73535514 MS.gene48797:intron 15.0%
!! ATTGAAATTGAAATGATAAT+TGG + chr4.4:73535408-73535427 MS.gene48797:intron 15.0%
!! TAATTAGGTATTATTATAAC+AGG + chr4.4:73535494-73535513 MS.gene48797:intron 15.0%
!!! GGGTTTTTTTTATAACTAAA+AGG + chr4.4:73535515-73535534 MS.gene48797:intron 20.0%
!!! TTGAAGCTTTATAAAATAAG+TGG + chr4.4:73535553-73535572 MS.gene48797:intron 20.0%
! AAAGAACTATCTATATGCTT+AGG - chr4.4:73535722-73535741 None:intergenic 25.0%
! GAAATTGAAATGATAATTGG+AGG + chr4.4:73535411-73535430 MS.gene48797:intron 25.0%
! TTCTCATTCCATTGTAAATA+AGG - chr4.4:73535309-73535328 None:intergenic 25.0%
!! ATGAGTGATTTATGCAATTT+TGG + chr4.4:73535585-73535604 MS.gene48797:intron 25.0%
!! GATTCAAACCTTATTTACAA+TGG + chr4.4:73535298-73535317 MS.gene48797:intron 25.0%
!! TTCAATGTCTCATTCAATTT+TGG - chr4.4:73535394-73535413 None:intergenic 25.0%
!!! GTACATGTGTCATTTTAATT+AGG + chr4.4:73535479-73535498 MS.gene48797:intron 25.0%
AACTATCTATATGCTTAGGA+AGG - chr4.4:73535718-73535737 None:intergenic 30.0%
ACAAACATACCTATTACAGT+TGG - chr4.4:73535153-73535172 None:intergenic 30.0%
ACTATCTATATGCTTAGGAA+GGG - chr4.4:73535717-73535736 None:intergenic 30.0%
ACTGTTAGTTGAAAACTTCT+AGG + chr4.4:73535276-73535295 MS.gene48797:intron 30.0%
ATTGAAATGATAATTGGAGG+AGG + chr4.4:73535414-73535433 MS.gene48797:intron 30.0%
GATAAGTAATGCATGGAATA+AGG - chr4.4:73534926-73534945 None:intergenic 30.0%
!! GTAGAAGTGCTAAACTTATA+GGG - chr4.4:73535131-73535150 None:intergenic 30.0%
AAGCTTGAGAGTTACAATTG+TGG + chr4.4:73534827-73534846 MS.gene48797:CDS 35.0%
CCATGCATTACTTATCTTCA+AGG + chr4.4:73534930-73534949 MS.gene48797:CDS 35.0%
CCTTGAAGATAAGTAATGCA+TGG - chr4.4:73534933-73534952 None:intergenic 35.0%
TGCATTACTTATCTTCAAGG+AGG + chr4.4:73534933-73534952 MS.gene48797:CDS 35.0%
! GTTGTTATTCAAACGAGCAA+TGG - chr4.4:73535069-73535088 None:intergenic 35.0%
! TTTTAACACCACATTTGCCA+GGG - chr4.4:73535106-73535125 None:intergenic 35.0%
! TTTTGGTCATCATCTTAGCT+TGG - chr4.4:73535377-73535396 None:intergenic 35.0%
!! ATTTTAACACCACATTTGCC+AGG - chr4.4:73535107-73535126 None:intergenic 35.0%
!! GGTAGAAGTGCTAAACTTAT+AGG - chr4.4:73535132-73535151 None:intergenic 35.0%
GCACTTCTACCAACTGTAAT+AGG + chr4.4:73535141-73535160 MS.gene48797:CDS 40.0%
TGTGGTTGCAATTATGTGCA+TGG + chr4.4:73534845-73534864 MS.gene48797:CDS 40.0%
ACTTGATGCTGCAATACAGC+AGG - chr4.4:73535802-73535821 None:intergenic 45.0%
ATCAACTAGTTGACCGCATG+TGG - chr4.4:73534901-73534920 None:intergenic 45.0%
GGTTGCAATTATGTGCATGG+TGG + chr4.4:73534848-73534867 MS.gene48797:CDS 45.0%
TGCAATTATGTGCATGGTGG+TGG + chr4.4:73534851-73534870 MS.gene48797:CDS 45.0%
! ACTTATCTTCAAGGAGGTGC+TGG + chr4.4:73534939-73534958 MS.gene48797:CDS 45.0%
! GGTCATCATCTTAGCTTGGA+GGG - chr4.4:73535373-73535392 None:intergenic 45.0%
! TGGTCATCATCTTAGCTTGG+AGG - chr4.4:73535374-73535393 None:intergenic 45.0%
!! AACTGCTTGAAAAGTGCTGC+TGG + chr4.4:73535041-73535060 MS.gene48797:CDS 45.0%
GGTTAGTGCACCAATAGCAG+AGG + chr4.4:73534872-73534891 MS.gene48797:CDS 50.0%
ATAGCAGAGGCAGCCACATG+CGG + chr4.4:73534885-73534904 MS.gene48797:CDS 55.0%
CACAGTGCTGTGCTGGAGTA+AGG + chr4.4:73534970-73534989 MS.gene48797:CDS 55.0%
CATGTGGCTGCCTCTGCTAT+TGG - chr4.4:73534885-73534904 None:intergenic 55.0%
CTTACTCCAGCACAGCACTG+TGG - chr4.4:73534972-73534991 None:intergenic 55.0%
AACCAAGCTGCTGCTCTCCC+TGG + chr4.4:73535086-73535105 MS.gene48797:CDS 60.0%
CCAGCACAGCACTGTGGAGA+TGG - chr4.4:73534966-73534985 None:intergenic 60.0%
CCATCTCCACAGTGCTGTGC+TGG + chr4.4:73534963-73534982 MS.gene48797:CDS 60.0%
GCTGCTCTCCCTGGCAAATG+TGG + chr4.4:73535095-73535114 MS.gene48797:CDS 60.0%
TTGCAAGCAGCCTTGCGAGC+CGG - chr4.4:73535023-73535042 None:intergenic 60.0%
CTTAACGCCGCCGCCAACAC+TGG + chr4.4:73534996-73535015 MS.gene48797:CDS 65.0%
TGCAAGCAGCCTTGCGAGCC+GGG - chr4.4:73535022-73535041 None:intergenic 65.0%
TGCCAGGGAGAGCAGCAGCT+TGG - chr4.4:73535091-73535110 None:intergenic 65.0%
CTTGCGAGCCGGGCCAGTGT+TGG - chr4.4:73535012-73535031 None:intergenic 70.0%
! CAACACTGGCCCGGCTCGCA+AGG + chr4.4:73535010-73535029 MS.gene48797:CDS 70.0%
! GCGAGCCGGGCCAGTGTTGG+CGG - chr4.4:73535009-73535028 None:intergenic 75.0%
!! AGCCGGGCCAGTGTTGGCGG+CGG - chr4.4:73535006-73535025 None:intergenic 75.0%
! CGCCGCCGCCAACACTGGCC+CGG + chr4.4:73535001-73535020 MS.gene48797:CDS 80.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 73534822 73535826 73534822 ID=MS.gene48797
chr4.4 mRNA 73534822 73535826 73534822 ID=MS.gene48797.t1;Parent=MS.gene48797
chr4.4 exon 73534822 73535162 73534822 ID=MS.gene48797.t1.exon1;Parent=MS.gene48797.t1
chr4.4 CDS 73534822 73535162 73534822 ID=cds.MS.gene48797.t1;Parent=MS.gene48797.t1
chr4.4 exon 73535814 73535826 73535814 ID=MS.gene48797.t1.exon2;Parent=MS.gene48797.t1
chr4.4 CDS 73535814 73535826 73535814 ID=cds.MS.gene48797.t1;Parent=MS.gene48797.t1
Gene Sequence

>MS.gene48797

ATGGCAAGCTTGAGAGTTACAATTGTGGTTGCAATTATGTGCATGGTGGTGGTTAGTGCACCAATAGCAGAGGCAGCCACATGCGGTCAACTAGTTGATAGCCTTATTCCATGCATTACTTATCTTCAAGGAGGTGCTGGTCCATCTCCACAGTGCTGTGCTGGAGTAAGGAAACTTAACGCCGCCGCCAACACTGGCCCGGCTCGCAAGGCTGCTTGCAACTGCTTGAAAAGTGCTGCTGGTACCATTGCTCGTTTGAATAACAACCAAGCTGCTGCTCTCCCTGGCAAATGTGGTGTTAAAATTCCCTATAAGTTTAGCACTTCTACCAACTGTAATAGGTATGTTTGTTTTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAACTCTTTTCATGCATCTTCTTGAACTGTTAGTTGAAAACTTCTAGGATTCAAACCTTATTTACAATGGAATGAGAAAAATTATTTGTTTCATATCATATTCTGAAATTGAAGATGTGCCCTCCAAGCTAAGATGATGACCAAAATTGAATGAGACATTGAAATTGAAATGATAATTGGAGGAGGTCAAATATGTGTCATTTATTTATTTTTTTTGGTATAATATATGTACATGTGTCATTTTAATTAGGTATTATTATAACAGGGTTTTTTTTATAACTAAAAGGTATGAAAAAAAAGAGTTGAAGCTTTATAAAATAAGTGGAAACAAATTATGAGTGATTTATGCAATTTTGGACAGTCATCAAAATGAAAGTAATACTATAAAATTAATAGATAAAATTATAAAATAATCTCAATTTTCATTTGACTTGTGCTTTATTTTTTATTTCATATATATTAGCCCTTCCTAAGCATATAGATAGTTCTTTTATTTTTCTCTCTCATATTTTAAATTGAAAATCATATATAACTTAATTTGCTTGTTTCCTGCTGTATTGCAGCATCAAGTTCTAA

Protein sequence

>MS.gene48797.t1

MASLRVTIVVAIMCMVVVSAPIAEAATCGQLVDSLIPCITYLQGGAGPSPQCCAGVRKLNAAANTGPARKAACNCLKSAAGTIARLNNNQAAALPGKCGVKIPYKFSTSTNCNSIKF