Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77022.t1 | XP_003605349.1 | 90.7 | 118 | 10 | 1 | 1 | 118 | 1 | 117 | 1.80E-50 | 208.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77022.t1 | A0AT28 | 66.1 | 118 | 40 | 0 | 1 | 118 | 1 | 118 | 2.6e-37 | 156.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77022.t1 | G7JJK0 | 90.7 | 118 | 10 | 1 | 1 | 118 | 1 | 117 | 1.3e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene77022 | 0.820461 | 6.78E-53 | -1.69E-46 |
| MS.gene049151 | MS.gene77022 | 0.806615 | 7.55E-50 | -1.69E-46 |
| MS.gene049153 | MS.gene77022 | 0.808674 | 2.76E-50 | -1.69E-46 |
| MS.gene049260 | MS.gene77022 | 0.85299 | 3.21E-61 | -1.69E-46 |
| MS.gene049346 | MS.gene77022 | 0.828676 | 7.88E-55 | -1.69E-46 |
| MS.gene049758 | MS.gene77022 | 0.86156 | 9.42E-64 | -1.69E-46 |
| MS.gene050128 | MS.gene77022 | 0.873887 | 1.04E-67 | -1.69E-46 |
| MS.gene050301 | MS.gene77022 | 0.865284 | 6.60E-65 | -1.69E-46 |
| MS.gene050432 | MS.gene77022 | 0.80118 | 1.02E-48 | -1.69E-46 |
| MS.gene05101 | MS.gene77022 | 0.813841 | 2.09E-51 | -1.69E-46 |
| MS.gene051471 | MS.gene77022 | 0.817949 | 2.53E-52 | -1.69E-46 |
| MS.gene051789 | MS.gene77022 | 0.821862 | 3.22E-53 | -1.69E-46 |
| MS.gene051860 | MS.gene77022 | 0.801499 | 8.75E-49 | -1.69E-46 |
| MS.gene052078 | MS.gene77022 | 0.813151 | 2.96E-51 | -1.69E-46 |
| MS.gene052178 | MS.gene77022 | 0.888202 | 7.28E-73 | -1.69E-46 |
| MS.gene052188 | MS.gene77022 | 0.803486 | 3.41E-49 | -1.69E-46 |
| MS.gene052253 | MS.gene77022 | 0.803442 | 3.48E-49 | -1.69E-46 |
| MS.gene052287 | MS.gene77022 | 0.822314 | 2.53E-53 | -1.69E-46 |
| MS.gene052340 | MS.gene77022 | 0.833224 | 6.03E-56 | -1.69E-46 |
| MS.gene053073 | MS.gene77022 | 0.804641 | 1.96E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene77022 | 0.812471 | 4.17E-51 | -1.69E-46 |
| MS.gene053204 | MS.gene77022 | 0.827759 | 1.31E-54 | -1.69E-46 |
| MS.gene053209 | MS.gene77022 | 0.834793 | 2.44E-56 | -1.69E-46 |
| MS.gene053557 | MS.gene77022 | 0.856537 | 3.01E-62 | -1.69E-46 |
| MS.gene053836 | MS.gene77022 | 0.845201 | 4.71E-59 | -1.69E-46 |
| MS.gene053928 | MS.gene77022 | 0.81125 | 7.69E-51 | -1.69E-46 |
| MS.gene054010 | MS.gene77022 | 0.8236 | 1.27E-53 | -1.69E-46 |
| MS.gene054011 | MS.gene77022 | 0.838127 | 3.46E-57 | -1.69E-46 |
| MS.gene054012 | MS.gene77022 | 0.831856 | 1.32E-55 | -1.69E-46 |
| MS.gene054013 | MS.gene77022 | 0.830857 | 2.32E-55 | -1.69E-46 |
| MS.gene054184 | MS.gene77022 | 0.805072 | 1.59E-49 | -1.69E-46 |
| MS.gene054185 | MS.gene77022 | 0.810029 | 1.41E-50 | -1.69E-46 |
| MS.gene054214 | MS.gene77022 | 0.868134 | 8.18E-66 | -1.69E-46 |
| MS.gene054618 | MS.gene77022 | 0.811547 | 6.63E-51 | -1.69E-46 |
| MS.gene055277 | MS.gene77022 | 0.862551 | 4.68E-64 | -1.69E-46 |
| MS.gene055303 | MS.gene77022 | 0.804289 | 2.32E-49 | -1.69E-46 |
| MS.gene055390 | MS.gene77022 | 0.838621 | 2.58E-57 | -1.69E-46 |
| MS.gene055422 | MS.gene77022 | 0.842738 | 2.15E-58 | -1.69E-46 |
| MS.gene055428 | MS.gene77022 | 0.818292 | 2.11E-52 | -1.69E-46 |
| MS.gene055604 | MS.gene77022 | 0.860967 | 1.43E-63 | -1.69E-46 |
| MS.gene056069 | MS.gene77022 | -0.805484 | 1.31E-49 | -1.69E-46 |
| MS.gene056191 | MS.gene77022 | 0.829046 | 6.41E-55 | -1.69E-46 |
| MS.gene056612 | MS.gene77022 | 0.834121 | 3.60E-56 | -1.69E-46 |
| MS.gene056772 | MS.gene77022 | 0.832901 | 7.25E-56 | -1.69E-46 |
| MS.gene056902 | MS.gene77022 | 0.814573 | 1.44E-51 | -1.69E-46 |
| MS.gene056931 | MS.gene77022 | 0.806122 | 9.59E-50 | -1.69E-46 |
| MS.gene056933 | MS.gene77022 | 0.801793 | 7.61E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene77022 | 0.820028 | 8.51E-53 | -1.69E-46 |
| MS.gene057121 | MS.gene77022 | 0.807405 | 5.14E-50 | -1.69E-46 |
| MS.gene05733 | MS.gene77022 | 0.800874 | 1.17E-48 | -1.69E-46 |
| MS.gene057448 | MS.gene77022 | 0.819388 | 1.19E-52 | -1.69E-46 |
| MS.gene057449 | MS.gene77022 | 0.812342 | 4.45E-51 | -1.69E-46 |
| MS.gene057450 | MS.gene77022 | 0.82707 | 1.92E-54 | -1.69E-46 |
| MS.gene057469 | MS.gene77022 | 0.816292 | 5.96E-52 | -1.69E-46 |
| MS.gene057752 | MS.gene77022 | 0.808244 | 3.41E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene77022 | 0.854337 | 1.32E-61 | -1.69E-46 |
| MS.gene05811 | MS.gene77022 | 0.851444 | 8.84E-61 | -1.69E-46 |
| MS.gene058142 | MS.gene77022 | 0.812482 | 4.15E-51 | -1.69E-46 |
| MS.gene058365 | MS.gene77022 | 0.802859 | 4.59E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene77022 | 0.817023 | 4.09E-52 | -1.69E-46 |
| MS.gene059228 | MS.gene77022 | 0.842141 | 3.10E-58 | -1.69E-46 |
| MS.gene059294 | MS.gene77022 | 0.806986 | 6.30E-50 | -1.69E-46 |
| MS.gene059518 | MS.gene77022 | 0.845868 | 3.11E-59 | -1.69E-46 |
| MS.gene059597 | MS.gene77022 | 0.804738 | 1.87E-49 | -1.69E-46 |
| MS.gene059688 | MS.gene77022 | 0.815809 | 7.64E-52 | -1.69E-46 |
| MS.gene059692 | MS.gene77022 | 0.839232 | 1.79E-57 | -1.69E-46 |
| MS.gene059695 | MS.gene77022 | 0.835296 | 1.82E-56 | -1.69E-46 |
| MS.gene05984 | MS.gene77022 | 0.832476 | 9.25E-56 | -1.69E-46 |
| MS.gene059894 | MS.gene77022 | 0.856939 | 2.29E-62 | -1.69E-46 |
| MS.gene059974 | MS.gene77022 | 0.858026 | 1.09E-62 | -1.69E-46 |
| MS.gene060005 | MS.gene77022 | 0.832069 | 1.17E-55 | -1.69E-46 |
| MS.gene06037 | MS.gene77022 | 0.857574 | 1.49E-62 | -1.69E-46 |
| MS.gene060407 | MS.gene77022 | 0.802672 | 5.02E-49 | -1.69E-46 |
| MS.gene060476 | MS.gene77022 | 0.836111 | 1.13E-56 | -1.69E-46 |
| MS.gene060495 | MS.gene77022 | 0.801342 | 9.42E-49 | -1.69E-46 |
| MS.gene060559 | MS.gene77022 | 0.802961 | 4.38E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene77022 | 0.884221 | 2.31E-71 | -1.69E-46 |
| MS.gene061112 | MS.gene77022 | 0.831493 | 1.62E-55 | -1.69E-46 |
| MS.gene061265 | MS.gene77022 | 0.827514 | 1.50E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene53311 | MS.gene77022 | PPI |
| MS.gene77022 | MS.gene76714 | PPI |
| MS.gene53305 | MS.gene77022 | PPI |
| MS.gene82256 | MS.gene77022 | PPI |
| MS.gene48835 | MS.gene77022 | PPI |
| MS.gene77022 | MS.gene48797 | PPI |
| MS.gene77022 | MS.gene82219 | PPI |
| MS.gene76713 | MS.gene77022 | PPI |
| MS.gene77022 | MS.gene76747 | PPI |
| MS.gene77025 | MS.gene77022 | PPI |
| MS.gene53312 | MS.gene77022 | PPI |
| MS.gene77022 | MS.gene82221 | PPI |
| MS.gene48828 | MS.gene77022 | PPI |
| MS.gene77022 | MS.gene48796 | PPI |
| MS.gene76705 | MS.gene77022 | PPI |
| MS.gene77022 | MS.gene82253 | PPI |
| MS.gene77022 | MS.gene76710 | PPI |
| MS.gene77022 | MS.gene48829 | PPI |
| MS.gene77022 | MS.gene76745 | PPI |
| MS.gene48831 | MS.gene77022 | PPI |
| MS.gene77022 | MS.gene82256 | PPI |
| MS.gene77022 | MS.gene82222 | PPI |
| MS.gene77022 | MS.gene53309 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77022.t1 | MTR_4g029390 | 90.678 | 118 | 10 | 1 | 1 | 118 | 1 | 117 | 2.35e-72 | 211 |
| MS.gene77022.t1 | MTR_4g029350 | 81.356 | 118 | 21 | 1 | 1 | 118 | 1 | 117 | 4.07e-65 | 193 |
| MS.gene77022.t1 | MTR_4g428370 | 70.085 | 117 | 33 | 1 | 1 | 117 | 58 | 172 | 3.11e-51 | 160 |
| MS.gene77022.t1 | MTR_4g027840 | 65.812 | 117 | 38 | 1 | 1 | 117 | 1 | 115 | 1.83e-48 | 151 |
| MS.gene77022.t1 | MTR_4g027800 | 64.957 | 117 | 39 | 1 | 1 | 117 | 1 | 115 | 2.57e-48 | 150 |
| MS.gene77022.t1 | MTR_4g029210 | 49.558 | 113 | 55 | 2 | 1 | 113 | 1 | 111 | 2.12e-28 | 100 |
| MS.gene77022.t1 | MTR_4g028180 | 48.246 | 114 | 51 | 4 | 11 | 117 | 14 | 126 | 1.35e-25 | 93.6 |
| MS.gene77022.t1 | MTR_2g062600 | 39.316 | 117 | 67 | 3 | 1 | 116 | 1 | 114 | 1.01e-23 | 88.2 |
| MS.gene77022.t1 | MTR_7g072930 | 44.860 | 107 | 55 | 3 | 11 | 115 | 13 | 117 | 1.49e-23 | 89.0 |
| MS.gene77022.t1 | MTR_7g072830 | 35.294 | 119 | 71 | 2 | 4 | 116 | 1 | 119 | 5.68e-22 | 84.0 |
| MS.gene77022.t1 | MTR_7g073170 | 40.367 | 109 | 62 | 3 | 11 | 118 | 13 | 119 | 8.54e-22 | 83.6 |
| MS.gene77022.t1 | MTR_7g073150 | 43.860 | 114 | 59 | 4 | 4 | 115 | 7 | 117 | 1.45e-21 | 83.2 |
| MS.gene77022.t1 | MTR_4g028190 | 37.815 | 119 | 72 | 2 | 1 | 118 | 5 | 122 | 5.55e-21 | 81.6 |
| MS.gene77022.t1 | MTR_3g467100 | 33.628 | 113 | 74 | 1 | 5 | 117 | 3 | 114 | 2.01e-20 | 79.7 |
| MS.gene77022.t1 | MTR_7g072730 | 34.746 | 118 | 71 | 2 | 4 | 115 | 1 | 118 | 3.01e-20 | 80.9 |
| MS.gene77022.t1 | MTR_4g028250 | 35.593 | 118 | 75 | 1 | 1 | 117 | 1 | 118 | 2.89e-19 | 77.0 |
| MS.gene77022.t1 | MTR_7g073120 | 35.345 | 116 | 71 | 3 | 1 | 114 | 5 | 118 | 1.46e-16 | 70.9 |
| MS.gene77022.t1 | MTR_7g072993 | 37.069 | 116 | 70 | 1 | 2 | 114 | 3 | 118 | 3.13e-16 | 69.7 |
| MS.gene77022.t1 | MTR_4g028310 | 32.500 | 120 | 76 | 3 | 1 | 117 | 1 | 118 | 1.36e-15 | 68.2 |
| MS.gene77022.t1 | MTR_3g046350 | 33.645 | 107 | 64 | 4 | 12 | 115 | 15 | 117 | 3.44e-15 | 66.6 |
| MS.gene77022.t1 | MTR_3g056640 | 31.967 | 122 | 77 | 4 | 1 | 117 | 2 | 122 | 5.29e-15 | 66.2 |
| MS.gene77022.t1 | MTR_7g072980 | 32.174 | 115 | 76 | 1 | 2 | 114 | 3 | 117 | 7.22e-15 | 66.2 |
| MS.gene77022.t1 | MTR_7g073130 | 34.211 | 114 | 73 | 2 | 2 | 114 | 3 | 115 | 1.63e-14 | 65.1 |
| MS.gene77022.t1 | MTR_3g046530 | 29.915 | 117 | 78 | 3 | 4 | 117 | 7 | 122 | 2.39e-14 | 64.7 |
| MS.gene77022.t1 | MTR_7g451500 | 33.696 | 92 | 60 | 1 | 26 | 116 | 7 | 98 | 2.94e-14 | 63.5 |
| MS.gene77022.t1 | MTR_7g072900 | 35.088 | 114 | 73 | 1 | 2 | 114 | 3 | 116 | 3.35e-14 | 63.9 |
| MS.gene77022.t1 | MTR_3g046515 | 30.435 | 115 | 76 | 3 | 4 | 115 | 7 | 120 | 4.45e-14 | 63.9 |
| MS.gene77022.t1 | MTR_3g046540 | 29.915 | 117 | 78 | 3 | 4 | 117 | 7 | 122 | 6.29e-14 | 63.5 |
| MS.gene77022.t1 | MTR_1g040335 | 34.454 | 119 | 71 | 3 | 4 | 116 | 1 | 118 | 6.90e-14 | 63.2 |
| MS.gene77022.t1 | MTR_7g072960 | 38.596 | 114 | 51 | 5 | 4 | 115 | 7 | 103 | 7.79e-14 | 63.5 |
| MS.gene77022.t1 | MTR_3g464130 | 35.556 | 90 | 55 | 2 | 27 | 114 | 18 | 106 | 2.89e-13 | 61.2 |
| MS.gene77022.t1 | MTR_3g464110 | 37.805 | 82 | 48 | 2 | 36 | 115 | 93 | 173 | 1.85e-12 | 60.8 |
| MS.gene77022.t1 | MTR_7g072810 | 36.449 | 107 | 59 | 3 | 11 | 115 | 13 | 112 | 1.95e-12 | 59.7 |
| MS.gene77022.t1 | MTR_4g028360 | 33.913 | 115 | 71 | 4 | 4 | 116 | 1 | 112 | 4.41e-12 | 58.9 |
| MS.gene77022.t1 | MTR_7g073060 | 32.759 | 116 | 77 | 1 | 2 | 116 | 3 | 118 | 1.85e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77022.t1 | AT5G59320 | 47.273 | 110 | 57 | 1 | 8 | 117 | 7 | 115 | 1.45e-28 | 100 |
| MS.gene77022.t1 | AT5G59310 | 46.364 | 110 | 55 | 2 | 8 | 117 | 7 | 112 | 1.43e-26 | 95.5 |
| MS.gene77022.t1 | AT3G51590 | 45.370 | 108 | 56 | 1 | 12 | 116 | 8 | 115 | 2.22e-25 | 92.8 |
| MS.gene77022.t1 | AT2G38540 | 48.276 | 116 | 56 | 4 | 4 | 117 | 5 | 118 | 3.31e-25 | 92.4 |
| MS.gene77022.t1 | AT3G51600 | 49.074 | 108 | 52 | 3 | 12 | 117 | 12 | 118 | 2.18e-24 | 90.1 |
| MS.gene77022.t1 | AT2G38530 | 46.087 | 115 | 60 | 2 | 4 | 117 | 5 | 118 | 3.92e-24 | 89.4 |
| MS.gene77022.t1 | AT5G01870 | 43.590 | 117 | 61 | 4 | 4 | 117 | 2 | 116 | 2.93e-23 | 87.4 |
| MS.gene77022.t1 | AT4G33355 | 43.519 | 108 | 57 | 1 | 13 | 116 | 11 | 118 | 3.21e-23 | 87.4 |
| MS.gene77022.t1 | AT4G33355 | 42.453 | 106 | 57 | 1 | 13 | 114 | 11 | 116 | 5.86e-22 | 84.0 |
| MS.gene77022.t1 | AT3G08770 | 44.330 | 97 | 50 | 3 | 24 | 117 | 18 | 113 | 1.63e-20 | 80.1 |
| MS.gene77022.t1 | AT3G08770 | 44.211 | 95 | 49 | 3 | 24 | 115 | 18 | 111 | 1.83e-19 | 77.4 |
| MS.gene77022.t1 | AT2G15050 | 39.286 | 112 | 62 | 4 | 12 | 117 | 11 | 122 | 3.90e-19 | 77.0 |
| MS.gene77022.t1 | AT2G15050 | 39.091 | 110 | 61 | 4 | 12 | 115 | 11 | 120 | 9.43e-18 | 73.6 |
| MS.gene77022.t1 | AT2G15050 | 39.806 | 103 | 58 | 3 | 12 | 110 | 11 | 113 | 2.99e-17 | 72.0 |
| MS.gene77022.t1 | AT2G18370 | 35.789 | 95 | 59 | 2 | 23 | 116 | 22 | 115 | 9.33e-16 | 68.2 |
Find 35 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCACTTCTACTAACTGTAAT+AGG | 0.249879 | 4.3:+70372504 | MS.gene77022:CDS |
| GCAGTTGCAAGCAGTCTTGC+GGG | 0.361401 | 4.3:-70372387 | None:intergenic |
| CTTGCGGGCGGGGCCGTTGT+TGG | 0.370391 | 4.3:-70372372 | None:intergenic |
| TGCAAGCAGTCTTGCGGGCG+GGG | 0.373033 | 4.3:-70372382 | None:intergenic |
| CCATGCATTACTTATCTTCA+AGG | 0.382464 | 4.3:+70372293 | MS.gene77022:CDS |
| AGTAGAAGTGCTAAACTTGT+AGG | 0.409303 | 4.3:-70372492 | None:intergenic |
| TTGCAAGCAGTCTTGCGGGC+GGG | 0.409587 | 4.3:-70372383 | None:intergenic |
| ACTTATCTTCAAGGTGGTGC+TGG | 0.410647 | 4.3:+70372302 | MS.gene77022:CDS |
| GTGACTGCTGCCTCTGCCAT+TGG | 0.435322 | 4.3:-70372242 | None:intergenic |
| GATAAGTAATGCATGGAGTA+AGG | 0.447792 | 4.3:-70372286 | None:intergenic |
| TGCAATTATGTGCATGGTGG+TGG | 0.466945 | 4.3:+70372211 | MS.gene77022:CDS |
| AACTGCTTGAAAAGTGCTGC+TGG | 0.487551 | 4.3:+70372404 | MS.gene77022:CDS |
| ATGTTAACACCACATTTGCC+AGG | 0.490963 | 4.3:-70372467 | None:intergenic |
| GCTGCTCTCCCTGGCAAATG+TGG | 0.495611 | 4.3:+70372458 | MS.gene77022:CDS |
| AGCAGTTGCAAGCAGTCTTG+CGG | 0.506600 | 4.3:-70372388 | None:intergenic |
| GTTGTTATTCAAACGAGCAA+TGG | 0.508618 | 4.3:-70372429 | None:intergenic |
| AACCAAGCTGCTGCTCTCCC+TGG | 0.514408 | 4.3:+70372449 | MS.gene77022:CDS |
| GGTGGAGTGAGGAGACTTAA+CGG | 0.519956 | 4.3:+70372344 | MS.gene77022:CDS |
| TGCCAGGGAGAGCAGCAGCT+TGG | 0.525683 | 4.3:-70372451 | None:intergenic |
| TTTGGCTGCAATTATGTGCA+TGG | 0.538143 | 4.3:+70372205 | MS.gene77022:CDS |
| GGTCCATCTGCAGCATGCTG+TGG | 0.539612 | 4.3:+70372323 | MS.gene77022:CDS |
| GTTGCAAGCAGTCTTGCGGG+CGG | 0.541246 | 4.3:-70372384 | None:intergenic |
| CCATCTGCAGCATGCTGTGG+TGG | 0.550747 | 4.3:+70372326 | MS.gene77022:CDS |
| GTAGAAGTGCTAAACTTGTA+GGG | 0.551668 | 4.3:-70372491 | None:intergenic |
| GTCACATGTGGACAAGTAGT+AGG | 0.553840 | 4.3:+70372260 | MS.gene77022:CDS |
| GGTTACTGCACCAATGGCAG+AGG | 0.568355 | 4.3:+70372232 | MS.gene77022:CDS |
| CCACCACAGCATGCTGCAGA+TGG | 0.580113 | 4.3:-70372326 | None:intergenic |
| GGTGGTGGTTACTGCACCAA+TGG | 0.601083 | 4.3:+70372226 | MS.gene77022:CDS |
| CCTTGAAGATAAGTAATGCA+TGG | 0.607580 | 4.3:-70372293 | None:intergenic |
| GCAGAGGCAGCAGTCACATG+TGG | 0.630368 | 4.3:+70372248 | MS.gene77022:CDS |
| CTTAACGGTGCTGCCAACAA+CGG | 0.636452 | 4.3:+70372359 | MS.gene77022:CDS |
| TGTTAACACCACATTTGCCA+GGG | 0.642773 | 4.3:-70372466 | None:intergenic |
| CAGCATGCTGTGGTGGAGTG+AGG | 0.648040 | 4.3:+70372333 | MS.gene77022:CDS |
| TGCATTACTTATCTTCAAGG+TGG | 0.657751 | 4.3:+70372296 | MS.gene77022:CDS |
| GGCTGCAATTATGTGCATGG+TGG | 0.657959 | 4.3:+70372208 | MS.gene77022:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTTTTTTTTTTAAAA+TGG | + | chr4.3:70372875-70372894 | MS.gene77022:intron | 0.0% |
| !! | GGCAAATTTAATAAGTTATT+CGG | - | chr4.3:70372835-70372854 | None:intergenic | 20.0% |
| ! | ACACACACATACATATATAT+AGG | - | chr4.3:70372740-70372759 | None:intergenic | 25.0% |
| ! | ATTTGCCTAAAAAAATGAAG+TGG | + | chr4.3:70372848-70372867 | MS.gene77022:intron | 25.0% |
| ! | CACACACATACATATATATA+GGG | - | chr4.3:70372739-70372758 | None:intergenic | 25.0% |
| ! | TGAGACATTGAAATGATAAT+TGG | + | chr4.3:70372994-70373013 | MS.gene77022:intron | 25.0% |
| !! | TCTCTAAGAATACTCTTTAA+CGG | + | chr4.3:70372578-70372597 | MS.gene77022:intron | 25.0% |
| !!! | AATCTTTAAAGAGTACCATT+AGG | + | chr4.3:70372703-70372722 | MS.gene77022:intron | 25.0% |
| !!! | ATCTTTAAAGAGTACCATTA+GGG | + | chr4.3:70372704-70372723 | MS.gene77022:intron | 25.0% |
| !!! | TTGTTCCACTTCATTTTTTT+AGG | - | chr4.3:70372856-70372875 | None:intergenic | 25.0% |
| ACTATCTATATGCTCATGAA+AGG | - | chr4.3:70373248-70373267 | None:intergenic | 30.0% | |
| ATTGAAATGATAATTGGAGG+AGG | + | chr4.3:70373000-70373019 | MS.gene77022:intron | 30.0% | |
| GACATTGAAATGATAATTGG+AGG | + | chr4.3:70372997-70373016 | MS.gene77022:intron | 30.0% | |
| ! | TTGAGTGACTTATGCATTTT+TGG | + | chr4.3:70373116-70373135 | MS.gene77022:intron | 30.0% |
| !! | TATTTTTGTTCCACTTCTTC+AGG | - | chr4.3:70372812-70372831 | None:intergenic | 30.0% |
| CCATGCATTACTTATCTTCA+AGG | + | chr4.3:70372293-70372312 | MS.gene77022:CDS | 35.0% | |
| CCTTGAAGATAAGTAATGCA+TGG | - | chr4.3:70372296-70372315 | None:intergenic | 35.0% | |
| GATAAGTAATGCATGGAGTA+AGG | - | chr4.3:70372289-70372308 | None:intergenic | 35.0% | |
| GCACTTCTACTAACTGTAAT+AGG | + | chr4.3:70372504-70372523 | MS.gene77022:CDS | 35.0% | |
| TGCATTACTTATCTTCAAGG+TGG | + | chr4.3:70372296-70372315 | MS.gene77022:CDS | 35.0% | |
| ! | AAGCATGAGAGTTGCATTTT+TGG | + | chr4.3:70372187-70372206 | MS.gene77022:CDS | 35.0% |
| ! | GTCTCACTCTCACTTAATTT+TGG | - | chr4.3:70372980-70372999 | None:intergenic | 35.0% |
| ! | GTTGTTATTCAAACGAGCAA+TGG | - | chr4.3:70372432-70372451 | None:intergenic | 35.0% |
| ! | TTTTTGGACACTCATCAAGA+TGG | + | chr4.3:70373132-70373151 | MS.gene77022:intron | 35.0% |
| !! | AGTAGAAGTGCTAAACTTGT+AGG | - | chr4.3:70372495-70372514 | None:intergenic | 35.0% |
| !! | GTAGAAGTGCTAAACTTGTA+GGG | - | chr4.3:70372494-70372513 | None:intergenic | 35.0% |
| !!! | TCATCATCTTTTTAGCTTGG+AGG | - | chr4.3:70372957-70372976 | None:intergenic | 35.0% |
| !!! | TGGTCATCATCTTTTTAGCT+TGG | - | chr4.3:70372960-70372979 | None:intergenic | 35.0% |
| ATGTTAACACCACATTTGCC+AGG | - | chr4.3:70372470-70372489 | None:intergenic | 40.0% | |
| TGTTAACACCACATTTGCCA+GGG | - | chr4.3:70372469-70372488 | None:intergenic | 40.0% | |
| TTTGGCTGCAATTATGTGCA+TGG | + | chr4.3:70372205-70372224 | MS.gene77022:CDS | 40.0% | |
| ! | CTTTAACGGTCTAAAGTAGC+AGG | + | chr4.3:70372592-70372611 | MS.gene77022:intron | 40.0% |
| ! | GTTTTCTGTATTGCAGCATC+AGG | + | chr4.3:70373326-70373345 | MS.gene77022:intron | 40.0% |
| !! | TAGGGTTTTGTGTTCCCTAA+TGG | - | chr4.3:70372721-70372740 | None:intergenic | 40.0% |
| ACTTATCTTCAAGGTGGTGC+TGG | + | chr4.3:70372302-70372321 | MS.gene77022:CDS | 45.0% | |
| ATCTCAAGTGCCTGAAGAAG+TGG | + | chr4.3:70372799-70372818 | MS.gene77022:intron | 45.0% | |
| GTCACATGTGGACAAGTAGT+AGG | + | chr4.3:70372260-70372279 | MS.gene77022:CDS | 45.0% | |
| TGCAATTATGTGCATGGTGG+TGG | + | chr4.3:70372211-70372230 | MS.gene77022:CDS | 45.0% | |
| !! | AACTGCTTGAAAAGTGCTGC+TGG | + | chr4.3:70372404-70372423 | MS.gene77022:CDS | 45.0% |
| !!! | TTTATATTTTATAAAATAAG+TGG | + | chr4.3:70373084-70373103 | MS.gene77022:intron | 5.0% |
| AGCAGTTGCAAGCAGTCTTG+CGG | - | chr4.3:70372391-70372410 | None:intergenic | 50.0% | |
| GGCTGCAATTATGTGCATGG+TGG | + | chr4.3:70372208-70372227 | MS.gene77022:CDS | 50.0% | |
| GGTGGAGTGAGGAGACTTAA+CGG | + | chr4.3:70372344-70372363 | MS.gene77022:CDS | 50.0% | |
| !! | CTTAACGGTGCTGCCAACAA+CGG | + | chr4.3:70372359-70372378 | MS.gene77022:CDS | 50.0% |
| GCAGTTGCAAGCAGTCTTGC+GGG | - | chr4.3:70372390-70372409 | None:intergenic | 55.0% | |
| GGTTACTGCACCAATGGCAG+AGG | + | chr4.3:70372232-70372251 | MS.gene77022:CDS | 55.0% | |
| ! | GGTGGTGGTTACTGCACCAA+TGG | + | chr4.3:70372226-70372245 | MS.gene77022:CDS | 55.0% |
| AACCAAGCTGCTGCTCTCCC+TGG | + | chr4.3:70372449-70372468 | MS.gene77022:CDS | 60.0% | |
| CCACCACAGCATGCTGCAGA+TGG | - | chr4.3:70372329-70372348 | None:intergenic | 60.0% | |
| CCATCTGCAGCATGCTGTGG+TGG | + | chr4.3:70372326-70372345 | MS.gene77022:CDS | 60.0% | |
| GCAGAGGCAGCAGTCACATG+TGG | + | chr4.3:70372248-70372267 | MS.gene77022:CDS | 60.0% | |
| GCTGCTCTCCCTGGCAAATG+TGG | + | chr4.3:70372458-70372477 | MS.gene77022:CDS | 60.0% | |
| GGTCCATCTGCAGCATGCTG+TGG | + | chr4.3:70372323-70372342 | MS.gene77022:CDS | 60.0% | |
| GTGACTGCTGCCTCTGCCAT+TGG | - | chr4.3:70372245-70372264 | None:intergenic | 60.0% | |
| GTTGCAAGCAGTCTTGCGGG+CGG | - | chr4.3:70372387-70372406 | None:intergenic | 60.0% | |
| TTGCAAGCAGTCTTGCGGGC+GGG | - | chr4.3:70372386-70372405 | None:intergenic | 60.0% | |
| ! | CAGCATGCTGTGGTGGAGTG+AGG | + | chr4.3:70372333-70372352 | MS.gene77022:CDS | 60.0% |
| TGCAAGCAGTCTTGCGGGCG+GGG | - | chr4.3:70372385-70372404 | None:intergenic | 65.0% | |
| TGCCAGGGAGAGCAGCAGCT+TGG | - | chr4.3:70372454-70372473 | None:intergenic | 65.0% | |
| CTTGCGGGCGGGGCCGTTGT+TGG | - | chr4.3:70372375-70372394 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 70372182 | 70373354 | 70372182 | ID=MS.gene77022 |
| chr4.3 | mRNA | 70372182 | 70373354 | 70372182 | ID=MS.gene77022.t1;Parent=MS.gene77022 |
| chr4.3 | exon | 70372182 | 70372525 | 70372182 | ID=MS.gene77022.t1.exon1;Parent=MS.gene77022.t1 |
| chr4.3 | CDS | 70372182 | 70372525 | 70372182 | ID=cds.MS.gene77022.t1;Parent=MS.gene77022.t1 |
| chr4.3 | exon | 70373342 | 70373354 | 70373342 | ID=MS.gene77022.t1.exon2;Parent=MS.gene77022.t1 |
| chr4.3 | CDS | 70373342 | 70373354 | 70373342 | ID=cds.MS.gene77022.t1;Parent=MS.gene77022.t1 |
| Gene Sequence |
| Protein sequence |
