AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76747


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76747.t1 MTR_4g029390 90.678 118 10 1 1 118 1 117 2.35e-72 211
MS.gene76747.t1 MTR_4g029350 81.356 118 21 1 1 118 1 117 4.07e-65 193
MS.gene76747.t1 MTR_4g428370 70.085 117 33 1 1 117 58 172 3.11e-51 160
MS.gene76747.t1 MTR_4g027840 65.812 117 38 1 1 117 1 115 1.83e-48 151
MS.gene76747.t1 MTR_4g027800 64.957 117 39 1 1 117 1 115 2.57e-48 150
MS.gene76747.t1 MTR_4g029210 49.558 113 55 2 1 113 1 111 2.12e-28 100
MS.gene76747.t1 MTR_4g028180 48.246 114 51 4 11 117 14 126 1.35e-25 93.6
MS.gene76747.t1 MTR_2g062600 39.316 117 67 3 1 116 1 114 1.01e-23 88.2
MS.gene76747.t1 MTR_7g072930 44.860 107 55 3 11 115 13 117 1.49e-23 89.0
MS.gene76747.t1 MTR_7g072830 35.294 119 71 2 4 116 1 119 5.68e-22 84.0
MS.gene76747.t1 MTR_7g073170 40.367 109 62 3 11 118 13 119 8.54e-22 83.6
MS.gene76747.t1 MTR_7g073150 43.860 114 59 4 4 115 7 117 1.45e-21 83.2
MS.gene76747.t1 MTR_4g028190 37.815 119 72 2 1 118 5 122 5.55e-21 81.6
MS.gene76747.t1 MTR_3g467100 33.628 113 74 1 5 117 3 114 2.01e-20 79.7
MS.gene76747.t1 MTR_7g072730 34.746 118 71 2 4 115 1 118 3.01e-20 80.9
MS.gene76747.t1 MTR_4g028250 35.593 118 75 1 1 117 1 118 2.89e-19 77.0
MS.gene76747.t1 MTR_7g073120 35.345 116 71 3 1 114 5 118 1.46e-16 70.9
MS.gene76747.t1 MTR_7g072993 37.069 116 70 1 2 114 3 118 3.13e-16 69.7
MS.gene76747.t1 MTR_4g028310 32.500 120 76 3 1 117 1 118 1.36e-15 68.2
MS.gene76747.t1 MTR_3g046350 33.645 107 64 4 12 115 15 117 3.44e-15 66.6
MS.gene76747.t1 MTR_3g056640 31.967 122 77 4 1 117 2 122 5.29e-15 66.2
MS.gene76747.t1 MTR_7g072980 32.174 115 76 1 2 114 3 117 7.22e-15 66.2
MS.gene76747.t1 MTR_7g073130 34.211 114 73 2 2 114 3 115 1.63e-14 65.1
MS.gene76747.t1 MTR_3g046530 29.915 117 78 3 4 117 7 122 2.39e-14 64.7
MS.gene76747.t1 MTR_7g451500 33.696 92 60 1 26 116 7 98 2.94e-14 63.5
MS.gene76747.t1 MTR_7g072900 35.088 114 73 1 2 114 3 116 3.35e-14 63.9
MS.gene76747.t1 MTR_3g046515 30.435 115 76 3 4 115 7 120 4.45e-14 63.9
MS.gene76747.t1 MTR_3g046540 29.915 117 78 3 4 117 7 122 6.29e-14 63.5
MS.gene76747.t1 MTR_1g040335 34.454 119 71 3 4 116 1 118 6.90e-14 63.2
MS.gene76747.t1 MTR_7g072960 38.596 114 51 5 4 115 7 103 7.79e-14 63.5
MS.gene76747.t1 MTR_3g464130 35.556 90 55 2 27 114 18 106 2.89e-13 61.2
MS.gene76747.t1 MTR_3g464110 37.805 82 48 2 36 115 93 173 1.85e-12 60.8
MS.gene76747.t1 MTR_7g072810 36.449 107 59 3 11 115 13 112 1.95e-12 59.7
MS.gene76747.t1 MTR_4g028360 33.913 115 71 4 4 116 1 112 4.41e-12 58.9
MS.gene76747.t1 MTR_7g073060 32.759 116 77 1 2 116 3 118 1.85e-11 57.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76747.t1 AT5G59320 47.273 110 57 1 8 117 7 115 1.45e-28 100
MS.gene76747.t1 AT5G59310 46.364 110 55 2 8 117 7 112 1.43e-26 95.5
MS.gene76747.t1 AT3G51590 45.370 108 56 1 12 116 8 115 2.22e-25 92.8
MS.gene76747.t1 AT2G38540 48.276 116 56 4 4 117 5 118 3.31e-25 92.4
MS.gene76747.t1 AT3G51600 49.074 108 52 3 12 117 12 118 2.18e-24 90.1
MS.gene76747.t1 AT2G38530 46.087 115 60 2 4 117 5 118 3.92e-24 89.4
MS.gene76747.t1 AT5G01870 43.590 117 61 4 4 117 2 116 2.93e-23 87.4
MS.gene76747.t1 AT4G33355 43.519 108 57 1 13 116 11 118 3.21e-23 87.4
MS.gene76747.t1 AT4G33355 42.453 106 57 1 13 114 11 116 5.86e-22 84.0
MS.gene76747.t1 AT3G08770 44.330 97 50 3 24 117 18 113 1.63e-20 80.1
MS.gene76747.t1 AT3G08770 44.211 95 49 3 24 115 18 111 1.83e-19 77.4
MS.gene76747.t1 AT2G15050 39.286 112 62 4 12 117 11 122 3.90e-19 77.0
MS.gene76747.t1 AT2G15050 39.091 110 61 4 12 115 11 120 9.43e-18 73.6
MS.gene76747.t1 AT2G15050 39.806 103 58 3 12 110 11 113 2.99e-17 72.0
MS.gene76747.t1 AT2G18370 35.789 95 59 2 23 116 22 115 9.33e-16 68.2

Find 37 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACTTCTACTAACTGTAAT+AGG 0.249879 4.1:+70674076 MS.gene76747:CDS
CTTGCGGGCGGGGCCGTTGT+TGG 0.344393 4.1:-70673944 None:intergenic
GCAGTTGCAAGCAGTCTTGC+GGG 0.361401 4.1:-70673959 None:intergenic
TGCAAGCAGTCTTGCGGGCG+GGG 0.373033 4.1:-70673954 None:intergenic
CCATGCATTACTTATCTTCA+AGG 0.382464 4.1:+70673865 MS.gene76747:CDS
AGTAGAAGTGCTAAACTTGT+AGG 0.409303 4.1:-70674064 None:intergenic
TTGCAAGCAGTCTTGCGGGC+GGG 0.409587 4.1:-70673955 None:intergenic
ACTTATCTTCAAGGTGGTGC+TGG 0.410647 4.1:+70673874 MS.gene76747:CDS
GTGACTGCTGCTTCTGCCAT+TGG 0.422408 4.1:-70673814 None:intergenic
GCGGGCGGGGCCGTTGTTGG+CGG 0.429798 4.1:-70673941 None:intergenic
GATAAGTAATGCATGGAGTA+AGG 0.447792 4.1:-70673858 None:intergenic
GTGGAGTGAGGAGACTTAAC+GGG 0.457847 4.1:+70673917 MS.gene76747:CDS
TGCAATTATGTGCATGGTGG+TGG 0.466945 4.1:+70673783 MS.gene76747:CDS
GGTGGAGTGAGGAGACTTAA+CGG 0.467248 4.1:+70673916 MS.gene76747:CDS
AACTGCTTGAAAAGTGCTGC+TGG 0.487551 4.1:+70673976 MS.gene76747:CDS
ATGTTAACACCACATTTGCC+AGG 0.490963 4.1:-70674039 None:intergenic
GCTGCTCTCCCTGGCAAATG+TGG 0.495611 4.1:+70674030 MS.gene76747:CDS
AGCAGTTGCAAGCAGTCTTG+CGG 0.506600 4.1:-70673960 None:intergenic
GTTGTTATTCAAACGAGCAA+TGG 0.508618 4.1:-70674001 None:intergenic
AACCAAGCTGCTGCTCTCCC+TGG 0.514408 4.1:+70674021 MS.gene76747:CDS
TGCCAGGGAGAGCAGCAGCT+TGG 0.525683 4.1:-70674023 None:intergenic
TTTGGCTGCAATTATGTGCA+TGG 0.538143 4.1:+70673777 MS.gene76747:CDS
GGTCCATCTGCAGCATGCTG+TGG 0.539612 4.1:+70673895 MS.gene76747:CDS
GTTGCAAGCAGTCTTGCGGG+CGG 0.541246 4.1:-70673956 None:intergenic
CCATCTGCAGCATGCTGTGG+TGG 0.550747 4.1:+70673898 MS.gene76747:CDS
GTAGAAGTGCTAAACTTGTA+GGG 0.551668 4.1:-70674063 None:intergenic
GTCACATGTGGACAAGTAGT+AGG 0.553840 4.1:+70673832 MS.gene76747:CDS
CCACCACAGCATGCTGCAGA+TGG 0.580113 4.1:-70673898 None:intergenic
CTTAACGGGGCCGCCAACAA+CGG 0.595696 4.1:+70673931 MS.gene76747:CDS
GGTGGTGGTTACTGCACCAA+TGG 0.601083 4.1:+70673798 MS.gene76747:CDS
CCTTGAAGATAAGTAATGCA+TGG 0.607580 4.1:-70673865 None:intergenic
GCAGAAGCAGCAGTCACATG+TGG 0.630368 4.1:+70673820 MS.gene76747:CDS
TGTTAACACCACATTTGCCA+GGG 0.642773 4.1:-70674038 None:intergenic
CAGCATGCTGTGGTGGAGTG+AGG 0.648040 4.1:+70673905 MS.gene76747:CDS
TGCATTACTTATCTTCAAGG+TGG 0.657751 4.1:+70673868 MS.gene76747:CDS
GGCTGCAATTATGTGCATGG+TGG 0.657959 4.1:+70673780 MS.gene76747:CDS
TGGAGTGAGGAGACTTAACG+GGG 0.697944 4.1:+70673918 MS.gene76747:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTTTTTTTTTTAAAA+TGG + chr4.1:70674448-70674467 MS.gene76747:intron 0.0%
!!! TTTATATTTTATAAAATAAA+TGG + chr4.1:70674656-70674675 MS.gene76747:intron 0.0%
!! CATATAATAATATTCTCAAA+TGG + chr4.1:70674494-70674513 MS.gene76747:intron 15.0%
!! ATTTCTCATATACGTAATTA+AGG - chr4.1:70674120-70674139 None:intergenic 20.0%
!! GGCAAATTTAATAAGTTATT+CGG - chr4.1:70674409-70674428 None:intergenic 20.0%
! ACACACACATACATATATAT+AGG - chr4.1:70674314-70674333 None:intergenic 25.0%
! ATTTGCCTAAAAAAATGAAG+TGG + chr4.1:70674422-70674441 MS.gene76747:intron 25.0%
! CACACACATACATATATATA+GGG - chr4.1:70674313-70674332 None:intergenic 25.0%
! TGAGACATTGAAATGATAAT+TGG + chr4.1:70674566-70674585 MS.gene76747:intron 25.0%
!! TCTCTAAGAATACTCTTTAA+CGG + chr4.1:70674150-70674169 MS.gene76747:intron 25.0%
!!! AATCTTTAAAGAGTACCATT+AGG + chr4.1:70674277-70674296 MS.gene76747:intron 25.0%
!!! ATCTTTAAAGAGTACCATTA+GGG + chr4.1:70674278-70674297 MS.gene76747:intron 25.0%
!!! TTGTTCCACTTCATTTTTTT+AGG - chr4.1:70674430-70674449 None:intergenic 25.0%
ATTGAAATGATAATTGGAGG+AGG + chr4.1:70674572-70674591 MS.gene76747:intron 30.0%
GACATTGAAATGATAATTGG+AGG + chr4.1:70674569-70674588 MS.gene76747:intron 30.0%
! ATGAGTGACTTATGCATTTT+TGG + chr4.1:70674688-70674707 MS.gene76747:intron 30.0%
! GTCTCACTTTCACTTAATTT+TGG - chr4.1:70674552-70674571 None:intergenic 30.0%
! TTTTTGGACAGTCATCAAAA+TGG + chr4.1:70674704-70674723 MS.gene76747:intron 30.0%
!! TATTTTTGTTCCACTTCTTC+AGG - chr4.1:70674386-70674405 None:intergenic 30.0%
AACTATCTATATGCTCGTGA+AGG - chr4.1:70674821-70674840 None:intergenic 35.0%
ACTATCTATATGCTCGTGAA+GGG - chr4.1:70674820-70674839 None:intergenic 35.0%
CCATGCATTACTTATCTTCA+AGG + chr4.1:70673865-70673884 MS.gene76747:CDS 35.0%
CCTTGAAGATAAGTAATGCA+TGG - chr4.1:70673868-70673887 None:intergenic 35.0%
GATAAGTAATGCATGGAGTA+AGG - chr4.1:70673861-70673880 None:intergenic 35.0%
GCACTTCTACTAACTGTAAT+AGG + chr4.1:70674076-70674095 MS.gene76747:CDS 35.0%
TGCATTACTTATCTTCAAGG+TGG + chr4.1:70673868-70673887 MS.gene76747:CDS 35.0%
TTAACGGTCTAAAAAGTAGC+AGG + chr4.1:70674166-70674185 MS.gene76747:intron 35.0%
! AAGCATGAGAGTTGCATTTT+TGG + chr4.1:70673759-70673778 MS.gene76747:CDS 35.0%
! GTTGTTATTCAAACGAGCAA+TGG - chr4.1:70674004-70674023 None:intergenic 35.0%
!! AGTAGAAGTGCTAAACTTGT+AGG - chr4.1:70674067-70674086 None:intergenic 35.0%
!! GTAGAAGTGCTAAACTTGTA+GGG - chr4.1:70674066-70674085 None:intergenic 35.0%
!!! TCATCATCTTTTTAGCTTGG+AGG - chr4.1:70674529-70674548 None:intergenic 35.0%
!!! TGGTCATCATCTTTTTAGCT+TGG - chr4.1:70674532-70674551 None:intergenic 35.0%
ATCTCAAGTACCTGAAGAAG+TGG + chr4.1:70674373-70674392 MS.gene76747:intron 40.0%
ATGTTAACACCACATTTGCC+AGG - chr4.1:70674042-70674061 None:intergenic 40.0%
TGTTAACACCACATTTGCCA+GGG - chr4.1:70674041-70674060 None:intergenic 40.0%
TTTGGCTGCAATTATGTGCA+TGG + chr4.1:70673777-70673796 MS.gene76747:CDS 40.0%
! GTTTTCTGTATTGCAGCATC+AGG + chr4.1:70674898-70674917 MS.gene76747:intron 40.0%
!! TAGGGTTTTGTGTTCCCTAA+TGG - chr4.1:70674295-70674314 None:intergenic 40.0%
ACTTATCTTCAAGGTGGTGC+TGG + chr4.1:70673874-70673893 MS.gene76747:CDS 45.0%
GTCACATGTGGACAAGTAGT+AGG + chr4.1:70673832-70673851 MS.gene76747:CDS 45.0%
TGCAATTATGTGCATGGTGG+TGG + chr4.1:70673783-70673802 MS.gene76747:CDS 45.0%
!! AACTGCTTGAAAAGTGCTGC+TGG + chr4.1:70673976-70673995 MS.gene76747:CDS 45.0%
AGCAGTTGCAAGCAGTCTTG+CGG - chr4.1:70673963-70673982 None:intergenic 50.0%
GGCTGCAATTATGTGCATGG+TGG + chr4.1:70673780-70673799 MS.gene76747:CDS 50.0%
GGTGGAGTGAGGAGACTTAA+CGG + chr4.1:70673916-70673935 MS.gene76747:CDS 50.0%
GTGGAGTGAGGAGACTTAAC+GGG + chr4.1:70673917-70673936 MS.gene76747:CDS 50.0%
TGGAGTGAGGAGACTTAACG+GGG + chr4.1:70673918-70673937 MS.gene76747:CDS 50.0%
GCAGAAGCAGCAGTCACATG+TGG + chr4.1:70673820-70673839 MS.gene76747:CDS 55.0%
GCAGTTGCAAGCAGTCTTGC+GGG - chr4.1:70673962-70673981 None:intergenic 55.0%
! GGTGGTGGTTACTGCACCAA+TGG + chr4.1:70673798-70673817 MS.gene76747:CDS 55.0%
! GTGACTGCTGCTTCTGCCAT+TGG - chr4.1:70673817-70673836 None:intergenic 55.0%
AACCAAGCTGCTGCTCTCCC+TGG + chr4.1:70674021-70674040 MS.gene76747:CDS 60.0%
CCACCACAGCATGCTGCAGA+TGG - chr4.1:70673901-70673920 None:intergenic 60.0%
CCATCTGCAGCATGCTGTGG+TGG + chr4.1:70673898-70673917 MS.gene76747:CDS 60.0%
CTTAACGGGGCCGCCAACAA+CGG + chr4.1:70673931-70673950 MS.gene76747:CDS 60.0%
GCTGCTCTCCCTGGCAAATG+TGG + chr4.1:70674030-70674049 MS.gene76747:CDS 60.0%
GGTCCATCTGCAGCATGCTG+TGG + chr4.1:70673895-70673914 MS.gene76747:CDS 60.0%
GTTGCAAGCAGTCTTGCGGG+CGG - chr4.1:70673959-70673978 None:intergenic 60.0%
TTGCAAGCAGTCTTGCGGGC+GGG - chr4.1:70673958-70673977 None:intergenic 60.0%
! CAGCATGCTGTGGTGGAGTG+AGG + chr4.1:70673905-70673924 MS.gene76747:CDS 60.0%
TGCAAGCAGTCTTGCGGGCG+GGG - chr4.1:70673957-70673976 None:intergenic 65.0%
TGCCAGGGAGAGCAGCAGCT+TGG - chr4.1:70674026-70674045 None:intergenic 65.0%
CTTGCGGGCGGGGCCGTTGT+TGG - chr4.1:70673947-70673966 None:intergenic 75.0%
! GCGGGCGGGGCCGTTGTTGG+CGG - chr4.1:70673944-70673963 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 70673754 70674926 70673754 ID=MS.gene76747
chr4.1 mRNA 70673754 70674926 70673754 ID=MS.gene76747.t1;Parent=MS.gene76747
chr4.1 exon 70673754 70674097 70673754 ID=MS.gene76747.t1.exon1;Parent=MS.gene76747.t1
chr4.1 CDS 70673754 70674097 70673754 ID=cds.MS.gene76747.t1;Parent=MS.gene76747.t1
chr4.1 exon 70674914 70674926 70674914 ID=MS.gene76747.t1.exon2;Parent=MS.gene76747.t1
chr4.1 CDS 70674914 70674926 70674914 ID=cds.MS.gene76747.t1;Parent=MS.gene76747.t1
Gene Sequence

>MS.gene76747

ATGGCAAGCATGAGAGTTGCATTTTTGGCTGCAATTATGTGCATGGTGGTGGTTACTGCACCAATGGCAGAAGCAGCAGTCACATGTGGACAAGTAGTAGGTTTCCTTACTCCATGCATTACTTATCTTCAAGGTGGTGCTGGTCCATCTGCAGCATGCTGTGGTGGAGTGAGGAGACTTAACGGGGCCGCCAACAACGGCCCCGCCCGCAAGACTGCTTGCAACTGCTTGAAAAGTGCTGCTGGTTCCATTGCTCGTTTGAATAACAACCAAGCTGCTGCTCTCCCTGGCAAATGTGGTGTTAACATTCCCTACAAGTTTAGCACTTCTACTAACTGTAATAGGTATGTTTTATATCTCACTCCTTAATTACGTATATGAGAAATGCTAACGAGTTCTCTAAGAATACTCTTTAACGGTCTAAAAAGTAGCAGGTTAATGATCTTGAAAATTTGAAACTTGCATATTCAATGCTTAAAAATTAAAGTGTTTTACATTTTTAGTGAATAATTTTCTAATTGAAAATCTTTAAAGAGTACCATTAGGGAACACAAAACCCTATATATATGTATGTGTGTGTGTTACTCTTGTCATGCATCTTCTTTAATTGTTAAGTGAGATCTCAAGTACCTGAAGAAGTGGAACAAAAATACCGAATAACTTATTAAATTTGCCTAAAAAAATGAAGTGGAACAATTTTTTTTTTTTTTAAAATGGAATGTGAAAAAAAATATTTATTTCATATAATAATATTCTCAAATGGAACATGTGTCCTCCAAGCTAAAAAGATGATGACCAAAATTAAGTGAAAGTGAGACATTGAAATGATAATTGGAGGAGGTCAATTATGTGTCATTTTTTTAATATAATATATGCGTATCATTTTATTTATCAAAACAGATTTTATATTTTATAAAATAAATGGAAACAAATTATGAGTGACTTATGCATTTTTGGACAGTCATCAAAATGGAAGTAATACAATAAAATTAATAAATAAAATTATGATATAATCTCAATTTTCATTTGAATTGTACAAATTTTTTTATTTCAAACATAGTATCCCTTCACGAGCATATAGATAGTTCTTTTATTTCTTTCTTTCTTATTTTAAATTGAATATCATATATAAGTTAATTTGTTTGTTTTCTGTATTGCAGCATCAGGTTCTAA

Protein sequence

>MS.gene76747.t1

MASMRVAFLAAIMCMVVVTAPMAEAAVTCGQVVGFLTPCITYLQGGAGPSAACCGGVRRLNGAANNGPARKTACNCLKSAAGSIARLNNNQAAALPGKCGVNIPYKFSTSTNCNSIRF