AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53305


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53305.t1 MTR_4g028180 96.032 126 4 1 1 125 1 126 6.50e-84 241
MS.gene53305.t1 MTR_7g073170 45.455 121 60 2 4 124 3 117 9.12e-32 109
MS.gene53305.t1 MTR_7g073150 44.167 120 62 2 4 123 3 117 4.34e-29 102
MS.gene53305.t1 MTR_4g428370 46.552 116 56 3 9 124 62 171 1.95e-28 102
MS.gene53305.t1 MTR_4g027800 41.880 117 62 3 9 125 5 115 7.49e-26 94.0
MS.gene53305.t1 MTR_7g072930 42.276 123 65 3 4 125 3 120 2.12e-25 94.0
MS.gene53305.t1 MTR_4g027840 42.735 117 61 3 9 125 5 115 2.33e-25 93.6
MS.gene53305.t1 MTR_4g028190 38.211 123 72 1 1 123 1 119 2.91e-25 92.8
MS.gene53305.t1 MTR_2g062600 38.333 120 67 3 6 124 1 114 3.09e-23 87.4
MS.gene53305.t1 MTR_4g029350 40.984 122 65 3 4 125 2 116 3.71e-23 87.4
MS.gene53305.t1 MTR_1g040335 44.715 123 61 3 4 125 3 119 9.45e-23 86.3
MS.gene53305.t1 MTR_7g072900 42.017 119 64 2 4 122 3 116 1.03e-22 86.3
MS.gene53305.t1 MTR_7g072830 38.211 123 70 3 4 125 3 120 2.26e-22 85.5
MS.gene53305.t1 MTR_7g072993 43.443 122 62 3 4 123 3 119 7.78e-22 84.3
MS.gene53305.t1 MTR_4g029390 40.984 122 65 3 4 125 2 116 1.16e-21 83.6
MS.gene53305.t1 MTR_7g073130 41.600 125 62 4 4 125 3 119 2.99e-21 82.8
MS.gene53305.t1 MTR_4g029210 38.053 113 64 3 9 121 5 111 1.41e-20 80.9
MS.gene53305.t1 MTR_7g072730 36.364 121 71 3 4 123 3 118 1.78e-20 81.6
MS.gene53305.t1 MTR_7g073060 36.066 122 73 2 4 125 3 119 1.35e-19 78.2
MS.gene53305.t1 MTR_7g072980 36.667 120 70 3 4 122 3 117 1.24e-18 76.3
MS.gene53305.t1 MTR_7g073120 37.600 125 70 4 1 123 1 119 1.49e-18 76.6
MS.gene53305.t1 MTR_7g073100 36.667 120 71 2 4 123 3 117 1.85e-18 76.3
MS.gene53305.t1 MTR_7g072960 38.710 124 54 3 6 125 1 106 2.12e-18 75.5
MS.gene53305.t1 MTR_7g072810 39.669 121 61 4 4 123 3 112 7.17e-18 73.9
MS.gene53305.t1 MTR_7g072760 37.097 124 72 3 4 125 3 122 3.01e-16 69.7
MS.gene53305.t1 MTR_7g073030 35.833 120 72 2 4 123 3 117 9.55e-16 69.3
MS.gene53305.t1 MTR_7g451500 29.787 94 62 1 31 124 9 98 1.58e-14 64.7
MS.gene53305.t1 MTR_3g467100 31.200 125 73 5 1 124 1 113 4.64e-13 61.2
MS.gene53305.t1 MTR_3g046515 28.455 123 82 2 2 123 3 120 6.59e-13 60.8
MS.gene53305.t1 MTR_3g056640 31.624 117 74 2 10 125 11 122 7.12e-13 60.8
MS.gene53305.t1 MTR_3g046530 28.000 125 84 2 2 125 3 122 1.19e-12 60.5
MS.gene53305.t1 MTR_3g046540 28.000 125 84 2 2 125 3 122 1.38e-12 60.1
MS.gene53305.t1 MTR_1g040330 37.398 123 70 3 4 125 3 119 1.66e-12 60.1
MS.gene53305.t1 MTR_4g028250 32.773 119 73 4 9 125 5 118 9.29e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53305.t1 AT2G15050 50.833 120 53 3 9 125 6 122 3.31e-32 110
MS.gene53305.t1 AT2G15050 50.847 118 52 3 9 123 6 120 2.86e-31 108
MS.gene53305.t1 AT2G38540 43.590 117 62 2 9 125 6 118 2.19e-29 103
MS.gene53305.t1 AT2G15050 51.351 111 48 3 9 116 6 113 2.25e-29 103
MS.gene53305.t1 AT5G01870 44.348 115 58 4 11 125 8 116 1.40e-28 100
MS.gene53305.t1 AT3G08770 48.515 101 48 3 25 125 17 113 5.23e-27 97.1
MS.gene53305.t1 AT3G08770 49.495 99 46 3 25 123 17 111 2.43e-26 95.5
MS.gene53305.t1 AT5G59320 46.847 111 53 3 15 125 11 115 1.48e-25 93.2
MS.gene53305.t1 AT5G59310 46.364 110 47 4 16 125 15 112 1.43e-24 90.9
MS.gene53305.t1 AT2G38530 42.735 117 63 2 9 125 6 118 8.72e-24 89.0
MS.gene53305.t1 AT3G51600 42.735 117 63 2 9 125 6 118 4.97e-23 87.0
MS.gene53305.t1 AT3G51590 39.316 117 65 3 9 125 6 116 2.33e-22 85.5
MS.gene53305.t1 AT2G18370 35.897 117 68 2 10 124 4 115 3.17e-20 79.7
MS.gene53305.t1 AT4G33355 34.426 122 73 3 4 125 5 119 1.95e-15 67.4
MS.gene53305.t1 AT4G33355 34.454 119 71 3 4 122 5 116 2.41e-14 64.7

Find 33 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTTTGAGCCATAGTATTA+AGG 0.282339 4.3:-71020766 None:intergenic
ATTGTGTTTCTTGTAACTTT+TGG 0.294759 4.3:+71020620 MS.gene53305:CDS
GGCAACTAGGCTTGTTTGTT+TGG 0.295657 4.3:+71020595 MS.gene53305:CDS
TACCTGTTACAGTCGGTATT+AGG 0.307547 4.3:-71020926 None:intergenic
ACCTGTTACAGTCGGTATTA+GGG 0.357260 4.3:-71020925 None:intergenic
GCCCTAATACCGACTGTAAC+AGG 0.378156 4.3:+71020924 MS.gene53305:CDS
GGGTATAAGCTTGTCACAAC+TGG 0.381388 4.3:-71020677 None:intergenic
GAAGACCAGCAGCAAGGTTA+AGG 0.386841 4.3:-71020865 None:intergenic
CATCATGGGTTACTCAACTA+TGG 0.397979 4.3:+71020574 None:intergenic
GTAACAGCTGCTTCAGCCTT+AGG 0.410392 4.3:-71020644 None:intergenic
AGTAAGGAACCTTAATACTA+TGG 0.425103 4.3:+71020757 MS.gene53305:CDS
AACAGCTCTACGATCATTTG+TGG 0.431202 4.3:-71020786 None:intergenic
AGTCGGTATTAGGGCTGATC+TGG 0.443403 4.3:-71020916 None:intergenic
TTAAACCTTAACCTTGCTGC+TGG 0.450896 4.3:+71020860 MS.gene53305:CDS
TGTGTTTCTTACATTATGAC+TGG 0.454062 4.3:+71020701 MS.gene53305:CDS
CACAGTGTTGTAATGGAGTA+AGG 0.467403 4.3:+71020741 MS.gene53305:CDS
GTTAACAGCATTCTTAATGC+AGG 0.496302 4.3:-71020813 None:intergenic
AAGAATGCTGTTAACCAAAG+TGG 0.504107 4.3:+71020821 MS.gene53305:CDS
GTTCCAGCACAGTGTTGTAA+TGG 0.512359 4.3:+71020734 MS.gene53305:CDS
ATGTTAACACCACATTTCCT+TGG 0.522311 4.3:-71020887 None:intergenic
GTATTAGGGCTGATCTGGTA+AGG 0.526182 4.3:-71020911 None:intergenic
TAGTGTAACTGAAACCACTT+TGG 0.541012 4.3:-71020835 None:intergenic
ACTCCATTACAACACTGTGC+TGG 0.541228 4.3:-71020737 None:intergenic
GCTTGTCACAACTGGACCAC+AGG 0.541996 4.3:-71020669 None:intergenic
ACCTTGCTGCTGGTCTTCCA+AGG 0.551966 4.3:+71020870 MS.gene53305:CDS
GTTACTCAACTATGGCAACT+AGG 0.565651 4.3:+71020582 MS.gene53305:CDS
GTTTCTTACATTATGACTGG+TGG 0.579604 4.3:+71020704 MS.gene53305:CDS
GCTGGTCTTCCAAGGAAATG+TGG 0.584199 4.3:+71020878 MS.gene53305:CDS
GAAGCAGCTGTTACATCCTG+TGG 0.593294 4.3:+71020653 MS.gene53305:CDS
TCCTTGGAAGACCAGCAGCA+AGG 0.622205 4.3:-71020871 None:intergenic
TAAGTAGTACCTGTTACAGT+CGG 0.645331 4.3:-71020933 None:intergenic
GTCATAATGTAAGAAACACA+AGG 0.653065 4.3:-71020698 None:intergenic
TCATAATGTAAGAAACACAA+GGG 0.708823 4.3:-71020697 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATACAGATATATACTAA+AGG - chr4.3:71020984-71021003 None:intergenic 15.0%
!! AAATACAGATATATACTAAA+GGG - chr4.3:71020983-71021002 None:intergenic 15.0%
!!! GTAACAAACTTTATTTTTTA+TGG + chr4.3:71021263-71021282 MS.gene53305:intron 15.0%
! TCATAATGTAAGAAACACAA+GGG - chr4.3:71020700-71020719 None:intergenic 25.0%
!! ATTGTGTTTCTTGTAACTTT+TGG + chr4.3:71020620-71020639 MS.gene53305:CDS 25.0%
AAGTGTGACAAGATTTAAAG+TGG + chr4.3:71021193-71021212 MS.gene53305:intron 30.0%
AATCTTGTCACACTTTAGTA+TGG - chr4.3:71021188-71021207 None:intergenic 30.0%
AGTAAGGAACCTTAATACTA+TGG + chr4.3:71020757-71020776 MS.gene53305:CDS 30.0%
GTCATAATGTAAGAAACACA+AGG - chr4.3:71020701-71020720 None:intergenic 30.0%
TATCATGTATGATCGAGATT+GGG + chr4.3:71021097-71021116 MS.gene53305:intron 30.0%
TGTGTTTCTTACATTATGAC+TGG + chr4.3:71020701-71020720 MS.gene53305:CDS 30.0%
TGTTTCCTATATATGTCTGA+CGG + chr4.3:71021290-71021309 MS.gene53305:intron 30.0%
AAAGACCGTCAGACATATAT+AGG - chr4.3:71021298-71021317 None:intergenic 35.0%
AAGAATGCTGTTAACCAAAG+TGG + chr4.3:71020821-71020840 MS.gene53305:CDS 35.0%
ATGTTAACACCACATTTCCT+TGG - chr4.3:71020890-71020909 None:intergenic 35.0%
GTATCATGTATGATCGAGAT+TGG + chr4.3:71021096-71021115 MS.gene53305:intron 35.0%
GTTAACAGCATTCTTAATGC+AGG - chr4.3:71020816-71020835 None:intergenic 35.0%
GTTTCTTACATTATGACTGG+TGG + chr4.3:71020704-71020723 MS.gene53305:CDS 35.0%
TAAGTAGTACCTGTTACAGT+CGG - chr4.3:71020936-71020955 None:intergenic 35.0%
TAGTGTAACTGAAACCACTT+TGG - chr4.3:71020838-71020857 None:intergenic 35.0%
TGGTTTGAGCCATAGTATTA+AGG - chr4.3:71020769-71020788 None:intergenic 35.0%
! GTGACAAGATTTAAAGTGGT+TGG + chr4.3:71021197-71021216 MS.gene53305:intron 35.0%
! TCTTGTAACTTTTGGTCCTA+AGG + chr4.3:71020628-71020647 MS.gene53305:CDS 35.0%
AACAGCTCTACGATCATTTG+TGG - chr4.3:71020789-71020808 None:intergenic 40.0%
ACCTGTTACAGTCGGTATTA+GGG - chr4.3:71020928-71020947 None:intergenic 40.0%
GATACGATCGAGAATGAGTT+GGG - chr4.3:71021081-71021100 None:intergenic 40.0%
GTTACTCAACTATGGCAACT+AGG + chr4.3:71020582-71020601 MS.gene53305:CDS 40.0%
TACCTGTTACAGTCGGTATT+AGG - chr4.3:71020929-71020948 None:intergenic 40.0%
TGATACGATCGAGAATGAGT+TGG - chr4.3:71021082-71021101 None:intergenic 40.0%
TTAAACCTTAACCTTGCTGC+TGG + chr4.3:71020860-71020879 MS.gene53305:CDS 40.0%
! CACAGTGTTGTAATGGAGTA+AGG + chr4.3:71020741-71020760 MS.gene53305:CDS 40.0%
ACTCCATTACAACACTGTGC+TGG - chr4.3:71020740-71020759 None:intergenic 45.0%
GGGTATAAGCTTGTCACAAC+TGG - chr4.3:71020680-71020699 None:intergenic 45.0%
GTATTAGGGCTGATCTGGTA+AGG - chr4.3:71020914-71020933 None:intergenic 45.0%
GTTCCAGCACAGTGTTGTAA+TGG + chr4.3:71020734-71020753 MS.gene53305:CDS 45.0%
TCATATGTAGCCCTGCAGAT+CGG + chr4.3:71021055-71021074 MS.gene53305:intron 45.0%
! GGCAACTAGGCTTGTTTGTT+TGG + chr4.3:71020595-71020614 MS.gene53305:CDS 45.0%
AGTCGGTATTAGGGCTGATC+TGG - chr4.3:71020919-71020938 None:intergenic 50.0%
GAAGACCAGCAGCAAGGTTA+AGG - chr4.3:71020868-71020887 None:intergenic 50.0%
GAAGCAGCTGTTACATCCTG+TGG + chr4.3:71020653-71020672 MS.gene53305:CDS 50.0%
GTAACAGCTGCTTCAGCCTT+AGG - chr4.3:71020647-71020666 None:intergenic 50.0%
! GCCCTAATACCGACTGTAAC+AGG + chr4.3:71020924-71020943 MS.gene53305:CDS 50.0%
!! GCTGGTCTTCCAAGGAAATG+TGG + chr4.3:71020878-71020897 MS.gene53305:CDS 50.0%
GCTTGTCACAACTGGACCAC+AGG - chr4.3:71020672-71020691 None:intergenic 55.0%
TCCTTGGAAGACCAGCAGCA+AGG - chr4.3:71020874-71020893 None:intergenic 55.0%
! AATGAGTTGGGCCGATCTGC+AGG - chr4.3:71021069-71021088 None:intergenic 55.0%
! ATGAGTTGGGCCGATCTGCA+GGG - chr4.3:71021068-71021087 None:intergenic 55.0%
!! ACCTTGCTGCTGGTCTTCCA+AGG + chr4.3:71020870-71020889 MS.gene53305:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 71020578 71021346 71020578 ID=MS.gene53305
chr4.3 mRNA 71020578 71021346 71020578 ID=MS.gene53305.t1;Parent=MS.gene53305
chr4.3 exon 71020578 71020945 71020578 ID=MS.gene53305.t1.exon1;Parent=MS.gene53305.t1
chr4.3 CDS 71020578 71020945 71020578 ID=cds.MS.gene53305.t1;Parent=MS.gene53305.t1
chr4.3 exon 71021337 71021346 71021337 ID=MS.gene53305.t1.exon2;Parent=MS.gene53305.t1
chr4.3 CDS 71021337 71021346 71021337 ID=cds.MS.gene53305.t1;Parent=MS.gene53305.t1
Gene Sequence

>MS.gene53305

ATGGGTTACTCAACTATGGCAACTAGGCTTGTTTGTTTGGCAATTGTGTTTCTTGTAACTTTTGGTCCTAAGGCTGAAGCAGCTGTTACATCCTGTGGTCCAGTTGTGACAAGCTTATACCCTTGTGTTTCTTACATTATGACTGGTGGAAACACTGTTCCAGCACAGTGTTGTAATGGAGTAAGGAACCTTAATACTATGGCTCAAACCACAAATGATCGTAGAGCTGTTTGTACCTGCATTAAGAATGCTGTTAACCAAAGTGGTTTCAGTTACACTAATTTAAACCTTAACCTTGCTGCTGGTCTTCCAAGGAAATGTGGTGTTAACATTCCTTACCAGATCAGCCCTAATACCGACTGTAACAGGTACTACTTATAATATTCACATATGTAATGTTAACCCTTTAGTATATATCTGTATTTTCTCACAAAAATAATTGCTTTTCTAATTTTCTCTAAAAAAATGCTACTTAAATCATATGTAGCCCTGCAGATCGGCCCAACTCATTCTCGATCGTATCATGTATGATCGAGATTGGGCTAGATCGAGATGTAGAGTTGTAGATAAGTCATTTTACTTTAATGATATGTTATGATATAAGATACCATACTAAAGTGTGACAAGATTTAAAGTGGTTGGAGTATTACAAGTTAAAAATGAGTTATTGATTTTTTTTTTTCATGTAACAAACTTTATTTTTTATGGAACATGTTTCCTATATATGTCTGACGGTCTTTTTTTGTTGTTTTGATGCAGTGTGCACTGA

Protein sequence

>MS.gene53305.t1

MGYSTMATRLVCLAIVFLVTFGPKAEAAVTSCGPVVTSLYPCVSYIMTGGNTVPAQCCNGVRNLNTMAQTTNDRRAVCTCIKNAVNQSGFSYTNLNLNLAAGLPRKCGVNIPYQISPNTDCNSVH