AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48835


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48835.t1 MTR_4g028180 96.032 126 4 1 1 125 1 126 6.50e-84 241
MS.gene48835.t1 MTR_7g073170 45.455 121 60 2 4 124 3 117 9.12e-32 109
MS.gene48835.t1 MTR_7g073150 44.167 120 62 2 4 123 3 117 4.34e-29 102
MS.gene48835.t1 MTR_4g428370 46.552 116 56 3 9 124 62 171 1.95e-28 102
MS.gene48835.t1 MTR_4g027800 41.880 117 62 3 9 125 5 115 7.49e-26 94.0
MS.gene48835.t1 MTR_7g072930 42.276 123 65 3 4 125 3 120 2.12e-25 94.0
MS.gene48835.t1 MTR_4g027840 42.735 117 61 3 9 125 5 115 2.33e-25 93.6
MS.gene48835.t1 MTR_4g028190 38.211 123 72 1 1 123 1 119 2.91e-25 92.8
MS.gene48835.t1 MTR_2g062600 38.333 120 67 3 6 124 1 114 3.09e-23 87.4
MS.gene48835.t1 MTR_4g029350 40.984 122 65 3 4 125 2 116 3.71e-23 87.4
MS.gene48835.t1 MTR_1g040335 44.715 123 61 3 4 125 3 119 9.45e-23 86.3
MS.gene48835.t1 MTR_7g072900 42.017 119 64 2 4 122 3 116 1.03e-22 86.3
MS.gene48835.t1 MTR_7g072830 38.211 123 70 3 4 125 3 120 2.26e-22 85.5
MS.gene48835.t1 MTR_7g072993 43.443 122 62 3 4 123 3 119 7.78e-22 84.3
MS.gene48835.t1 MTR_4g029390 40.984 122 65 3 4 125 2 116 1.16e-21 83.6
MS.gene48835.t1 MTR_7g073130 41.600 125 62 4 4 125 3 119 2.99e-21 82.8
MS.gene48835.t1 MTR_4g029210 38.053 113 64 3 9 121 5 111 1.41e-20 80.9
MS.gene48835.t1 MTR_7g072730 36.364 121 71 3 4 123 3 118 1.78e-20 81.6
MS.gene48835.t1 MTR_7g073060 36.066 122 73 2 4 125 3 119 1.35e-19 78.2
MS.gene48835.t1 MTR_7g072980 36.667 120 70 3 4 122 3 117 1.24e-18 76.3
MS.gene48835.t1 MTR_7g073120 37.600 125 70 4 1 123 1 119 1.49e-18 76.6
MS.gene48835.t1 MTR_7g073100 36.667 120 71 2 4 123 3 117 1.85e-18 76.3
MS.gene48835.t1 MTR_7g072960 38.710 124 54 3 6 125 1 106 2.12e-18 75.5
MS.gene48835.t1 MTR_7g072810 39.669 121 61 4 4 123 3 112 7.17e-18 73.9
MS.gene48835.t1 MTR_7g072760 37.097 124 72 3 4 125 3 122 3.01e-16 69.7
MS.gene48835.t1 MTR_7g073030 35.833 120 72 2 4 123 3 117 9.55e-16 69.3
MS.gene48835.t1 MTR_7g451500 29.787 94 62 1 31 124 9 98 1.58e-14 64.7
MS.gene48835.t1 MTR_3g467100 31.200 125 73 5 1 124 1 113 4.64e-13 61.2
MS.gene48835.t1 MTR_3g046515 28.455 123 82 2 2 123 3 120 6.59e-13 60.8
MS.gene48835.t1 MTR_3g056640 31.624 117 74 2 10 125 11 122 7.12e-13 60.8
MS.gene48835.t1 MTR_3g046530 28.000 125 84 2 2 125 3 122 1.19e-12 60.5
MS.gene48835.t1 MTR_3g046540 28.000 125 84 2 2 125 3 122 1.38e-12 60.1
MS.gene48835.t1 MTR_1g040330 37.398 123 70 3 4 125 3 119 1.66e-12 60.1
MS.gene48835.t1 MTR_4g028250 32.773 119 73 4 9 125 5 118 9.29e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48835.t1 AT2G15050 50.833 120 53 3 9 125 6 122 3.31e-32 110
MS.gene48835.t1 AT2G15050 50.847 118 52 3 9 123 6 120 2.86e-31 108
MS.gene48835.t1 AT2G38540 43.590 117 62 2 9 125 6 118 2.19e-29 103
MS.gene48835.t1 AT2G15050 51.351 111 48 3 9 116 6 113 2.25e-29 103
MS.gene48835.t1 AT5G01870 44.348 115 58 4 11 125 8 116 1.40e-28 100
MS.gene48835.t1 AT3G08770 48.515 101 48 3 25 125 17 113 5.23e-27 97.1
MS.gene48835.t1 AT3G08770 49.495 99 46 3 25 123 17 111 2.43e-26 95.5
MS.gene48835.t1 AT5G59320 46.847 111 53 3 15 125 11 115 1.48e-25 93.2
MS.gene48835.t1 AT5G59310 46.364 110 47 4 16 125 15 112 1.43e-24 90.9
MS.gene48835.t1 AT2G38530 42.735 117 63 2 9 125 6 118 8.72e-24 89.0
MS.gene48835.t1 AT3G51600 42.735 117 63 2 9 125 6 118 4.97e-23 87.0
MS.gene48835.t1 AT3G51590 39.316 117 65 3 9 125 6 116 2.33e-22 85.5
MS.gene48835.t1 AT2G18370 35.897 117 68 2 10 124 4 115 3.17e-20 79.7
MS.gene48835.t1 AT4G33355 34.426 122 73 3 4 125 5 119 1.95e-15 67.4
MS.gene48835.t1 AT4G33355 34.454 119 71 3 4 122 5 116 2.41e-14 64.7

Find 33 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTTTGAGCCATAGTATTA+AGG 0.282339 4.4:-74221897 None:intergenic
ATTGTGTTTCTTGTAACTTT+TGG 0.294759 4.4:+74221751 MS.gene48835:CDS
GGCAACTAGGCTTGTTTGTT+TGG 0.295657 4.4:+74221726 MS.gene48835:CDS
TACCTGTTACAGTCGGTATT+AGG 0.307547 4.4:-74222057 None:intergenic
ACCTGTTACAGTCGGTATTA+GGG 0.357260 4.4:-74222056 None:intergenic
GCCCTAATACCGACTGTAAC+AGG 0.378156 4.4:+74222055 MS.gene48835:CDS
GGGTATAAGCTTGTCACAAC+TGG 0.381388 4.4:-74221808 None:intergenic
GAAGACCAGCAGCAAGGTTA+AGG 0.386841 4.4:-74221996 None:intergenic
CATCATGGGTTACTCAACTA+TGG 0.397979 4.4:+74221705 None:intergenic
GTAACAGCTGCTTCAGCCTT+AGG 0.410392 4.4:-74221775 None:intergenic
AGTAAGGAACCTTAATACTA+TGG 0.425103 4.4:+74221888 MS.gene48835:CDS
AACAGCTCTACGATCATTTG+TGG 0.431202 4.4:-74221917 None:intergenic
AGTCGGTATTAGGGCTGATC+TGG 0.443403 4.4:-74222047 None:intergenic
TTAAACCTTAACCTTGCTGC+TGG 0.450896 4.4:+74221991 MS.gene48835:CDS
TGTGTTTCTTACATTATGAC+TGG 0.454062 4.4:+74221832 MS.gene48835:CDS
CACAGTGTTGTAATGGAGTA+AGG 0.461553 4.4:+74221872 MS.gene48835:CDS
GTTAACAGCATTCTTAATGC+AGG 0.496302 4.4:-74221944 None:intergenic
AAGAATGCTGTTAACCAAAG+TGG 0.504107 4.4:+74221952 MS.gene48835:CDS
ATGTTAACACCACATTTCCT+TGG 0.522311 4.4:-74222018 None:intergenic
GTATTAGGGCTGATCTGGTA+AGG 0.526182 4.4:-74222042 None:intergenic
TAGTGTAACTGAAACCACTT+TGG 0.541012 4.4:-74221966 None:intergenic
GTGCCTGCACAGTGTTGTAA+TGG 0.541888 4.4:+74221865 MS.gene48835:CDS
GCTTGTCACAACTGGACCAC+AGG 0.541996 4.4:-74221800 None:intergenic
ACCTTGCTGCTGGTCTTCCA+AGG 0.551966 4.4:+74222001 MS.gene48835:CDS
GTTACTCAACTATGGCAACT+AGG 0.565651 4.4:+74221713 MS.gene48835:CDS
GTTTCTTACATTATGACTGG+TGG 0.579604 4.4:+74221835 MS.gene48835:CDS
GCTGGTCTTCCAAGGAAATG+TGG 0.584199 4.4:+74222009 MS.gene48835:CDS
GAAGCAGCTGTTACATCCTG+TGG 0.593294 4.4:+74221784 MS.gene48835:CDS
ACTCCATTACAACACTGTGC+AGG 0.594186 4.4:-74221868 None:intergenic
TCCTTGGAAGACCAGCAGCA+AGG 0.622205 4.4:-74222002 None:intergenic
TAAGTAGTACCTGTTACAGT+CGG 0.645331 4.4:-74222064 None:intergenic
GTCATAATGTAAGAAACACA+AGG 0.653065 4.4:-74221829 None:intergenic
TCATAATGTAAGAAACACAA+GGG 0.708823 4.4:-74221828 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATACAGATATATACTAA+AGG - chr4.4:74222115-74222134 None:intergenic 15.0%
!! AAATACAGATATATACTAAA+GGG - chr4.4:74222114-74222133 None:intergenic 15.0%
!!! GTAACAAACTTTATTTTTTA+TGG + chr4.4:74222392-74222411 MS.gene48835:intron 15.0%
! TCATAATGTAAGAAACACAA+GGG - chr4.4:74221831-74221850 None:intergenic 25.0%
!! ATTGTGTTTCTTGTAACTTT+TGG + chr4.4:74221751-74221770 MS.gene48835:CDS 25.0%
AAGTGTGACAAGATTTAAAG+TGG + chr4.4:74222323-74222342 MS.gene48835:intron 30.0%
AATCTTGTCACACTTTAGTA+TGG - chr4.4:74222318-74222337 None:intergenic 30.0%
AGTAAGGAACCTTAATACTA+TGG + chr4.4:74221888-74221907 MS.gene48835:CDS 30.0%
GTCATAATGTAAGAAACACA+AGG - chr4.4:74221832-74221851 None:intergenic 30.0%
TATCATGTATGATCGAGATT+GGG + chr4.4:74222227-74222246 MS.gene48835:intron 30.0%
TGTGTTTCTTACATTATGAC+TGG + chr4.4:74221832-74221851 MS.gene48835:CDS 30.0%
TGTTTCCTATATATGTCTGA+CGG + chr4.4:74222419-74222438 MS.gene48835:intron 30.0%
AAAGACCGTCAGACATATAT+AGG - chr4.4:74222427-74222446 None:intergenic 35.0%
AAGAATGCTGTTAACCAAAG+TGG + chr4.4:74221952-74221971 MS.gene48835:CDS 35.0%
ATGTTAACACCACATTTCCT+TGG - chr4.4:74222021-74222040 None:intergenic 35.0%
GTATCATGTATGATCGAGAT+TGG + chr4.4:74222226-74222245 MS.gene48835:intron 35.0%
GTTAACAGCATTCTTAATGC+AGG - chr4.4:74221947-74221966 None:intergenic 35.0%
GTTTCTTACATTATGACTGG+TGG + chr4.4:74221835-74221854 MS.gene48835:CDS 35.0%
TAAGTAGTACCTGTTACAGT+CGG - chr4.4:74222067-74222086 None:intergenic 35.0%
TAGTGTAACTGAAACCACTT+TGG - chr4.4:74221969-74221988 None:intergenic 35.0%
TGGTTTGAGCCATAGTATTA+AGG - chr4.4:74221900-74221919 None:intergenic 35.0%
! GTGACAAGATTTAAAGTGGT+TGG + chr4.4:74222327-74222346 MS.gene48835:intron 35.0%
! TCTTGTAACTTTTGGTCCTA+AGG + chr4.4:74221759-74221778 MS.gene48835:CDS 35.0%
AACAGCTCTACGATCATTTG+TGG - chr4.4:74221920-74221939 None:intergenic 40.0%
ACCTGTTACAGTCGGTATTA+GGG - chr4.4:74222059-74222078 None:intergenic 40.0%
GATACGATCGAGAATGAGTT+GGG - chr4.4:74222211-74222230 None:intergenic 40.0%
GTTACTCAACTATGGCAACT+AGG + chr4.4:74221713-74221732 MS.gene48835:CDS 40.0%
TACCTGTTACAGTCGGTATT+AGG - chr4.4:74222060-74222079 None:intergenic 40.0%
TGATACGATCGAGAATGAGT+TGG - chr4.4:74222212-74222231 None:intergenic 40.0%
TTAAACCTTAACCTTGCTGC+TGG + chr4.4:74221991-74222010 MS.gene48835:CDS 40.0%
! CACAGTGTTGTAATGGAGTA+AGG + chr4.4:74221872-74221891 MS.gene48835:CDS 40.0%
ACTCCATTACAACACTGTGC+AGG - chr4.4:74221871-74221890 None:intergenic 45.0%
GGGTATAAGCTTGTCACAAC+TGG - chr4.4:74221811-74221830 None:intergenic 45.0%
GTATTAGGGCTGATCTGGTA+AGG - chr4.4:74222045-74222064 None:intergenic 45.0%
TCATATGTAGCCCTGCAGAT+CGG + chr4.4:74222185-74222204 MS.gene48835:intron 45.0%
! GGCAACTAGGCTTGTTTGTT+TGG + chr4.4:74221726-74221745 MS.gene48835:CDS 45.0%
AGTCGGTATTAGGGCTGATC+TGG - chr4.4:74222050-74222069 None:intergenic 50.0%
GAAGACCAGCAGCAAGGTTA+AGG - chr4.4:74221999-74222018 None:intergenic 50.0%
GAAGCAGCTGTTACATCCTG+TGG + chr4.4:74221784-74221803 MS.gene48835:CDS 50.0%
GTAACAGCTGCTTCAGCCTT+AGG - chr4.4:74221778-74221797 None:intergenic 50.0%
GTGCCTGCACAGTGTTGTAA+TGG + chr4.4:74221865-74221884 MS.gene48835:CDS 50.0%
! GCCCTAATACCGACTGTAAC+AGG + chr4.4:74222055-74222074 MS.gene48835:CDS 50.0%
!! GCTGGTCTTCCAAGGAAATG+TGG + chr4.4:74222009-74222028 MS.gene48835:CDS 50.0%
GCTTGTCACAACTGGACCAC+AGG - chr4.4:74221803-74221822 None:intergenic 55.0%
TCCTTGGAAGACCAGCAGCA+AGG - chr4.4:74222005-74222024 None:intergenic 55.0%
! AATGAGTTGGGCCGATCTGC+AGG - chr4.4:74222199-74222218 None:intergenic 55.0%
! ATGAGTTGGGCCGATCTGCA+GGG - chr4.4:74222198-74222217 None:intergenic 55.0%
!! ACCTTGCTGCTGGTCTTCCA+AGG + chr4.4:74222001-74222020 MS.gene48835:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 74221709 74222475 74221709 ID=MS.gene48835
chr4.4 mRNA 74221709 74222475 74221709 ID=MS.gene48835.t1;Parent=MS.gene48835
chr4.4 exon 74221709 74222076 74221709 ID=MS.gene48835.t1.exon1;Parent=MS.gene48835.t1
chr4.4 CDS 74221709 74222076 74221709 ID=cds.MS.gene48835.t1;Parent=MS.gene48835.t1
chr4.4 exon 74222466 74222475 74222466 ID=MS.gene48835.t1.exon2;Parent=MS.gene48835.t1
chr4.4 CDS 74222466 74222475 74222466 ID=cds.MS.gene48835.t1;Parent=MS.gene48835.t1
Gene Sequence

>MS.gene48835

ATGGGTTACTCAACTATGGCAACTAGGCTTGTTTGTTTGGCAATTGTGTTTCTTGTAACTTTTGGTCCTAAGGCTGAAGCAGCTGTTACATCCTGTGGTCCAGTTGTGACAAGCTTATACCCTTGTGTTTCTTACATTATGACTGGTGGAAACACTGTGCCTGCACAGTGTTGTAATGGAGTAAGGAACCTTAATACTATGGCTCAAACCACAAATGATCGTAGAGCTGTTTGTACCTGCATTAAGAATGCTGTTAACCAAAGTGGTTTCAGTTACACTAATTTAAACCTTAACCTTGCTGCTGGTCTTCCAAGGAAATGTGGTGTTAACATTCCTTACCAGATCAGCCCTAATACCGACTGTAACAGGTACTACTTATAATATTCACATATGTAATGTTAACCCTTTAGTATATATCTGTATTTTCTCACAAAAATAATTGCTTTTCTAATTTTCTCTAAAAAATGCTACTTAAATCATATGTAGCCCTGCAGATCGGCCCAACTCATTCTCGATCGTATCATGTATGATCGAGATTGGGCTAGATCGAGATGTAGAGTTGTAGATAAGTCATTTTACTTTAATGATATGTTATGATATAAGATACCATACTAAAGTGTGACAAGATTTAAAGTGGTTGGAGTACTACAAGTTAAAAATGAGTTATTGATTTTTTTTTTCATGTAACAAACTTTATTTTTTATGGAACATGTTTCCTATATATGTCTGACGGTCTTTTTTTGTTGTTTTGATGCAGTGTGCACTGA

Protein sequence

>MS.gene48835.t1

MGYSTMATRLVCLAIVFLVTFGPKAEAAVTSCGPVVTSLYPCVSYIMTGGNTVPAQCCNGVRNLNTMAQTTNDRRAVCTCIKNAVNQSGFSYTNLNLNLAAGLPRKCGVNIPYQISPNTDCNSVH