Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77025.t1 | XP_003605345.1 | 93.2 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 5.70E-52 | 213.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77025.t1 | A0AT33 | 70.9 | 110 | 30 | 2 | 9 | 117 | 2 | 110 | 1.7e-36 | 153.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77025.t1 | G7JJJ6 | 93.2 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 4.1e-52 | 213.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene77025 | 0.839194 | 1.83E-57 | -1.69E-46 |
| MS.gene049222 | MS.gene77025 | 0.808191 | 3.50E-50 | -1.69E-46 |
| MS.gene049285 | MS.gene77025 | 0.810049 | 1.40E-50 | -1.69E-46 |
| MS.gene049320 | MS.gene77025 | 0.810512 | 1.11E-50 | -1.69E-46 |
| MS.gene049373 | MS.gene77025 | 0.868794 | 5.01E-66 | -1.69E-46 |
| MS.gene049609 | MS.gene77025 | 0.861796 | 7.98E-64 | -1.69E-46 |
| MS.gene049633 | MS.gene77025 | 0.837089 | 6.39E-57 | -1.69E-46 |
| MS.gene049805 | MS.gene77025 | 0.807895 | 4.04E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene77025 | 0.827654 | 1.39E-54 | -1.69E-46 |
| MS.gene050375 | MS.gene77025 | 0.810361 | 1.20E-50 | -1.69E-46 |
| MS.gene050577 | MS.gene77025 | 0.809322 | 2.00E-50 | -1.69E-46 |
| MS.gene050593 | MS.gene77025 | 0.823976 | 1.04E-53 | -1.69E-46 |
| MS.gene050612 | MS.gene77025 | 0.800821 | 1.20E-48 | -1.69E-46 |
| MS.gene050699 | MS.gene77025 | 0.83326 | 5.90E-56 | -1.69E-46 |
| MS.gene050747 | MS.gene77025 | 0.845443 | 4.05E-59 | -1.69E-46 |
| MS.gene050772 | MS.gene77025 | 0.839053 | 1.99E-57 | -1.69E-46 |
| MS.gene05080 | MS.gene77025 | 0.819758 | 9.81E-53 | -1.69E-46 |
| MS.gene05081 | MS.gene77025 | 0.864574 | 1.10E-64 | -1.69E-46 |
| MS.gene050868 | MS.gene77025 | 0.824994 | 5.97E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene77025 | 0.823189 | 1.58E-53 | -1.69E-46 |
| MS.gene050950 | MS.gene77025 | 0.843946 | 1.02E-58 | -1.69E-46 |
| MS.gene050986 | MS.gene77025 | 0.814415 | 1.56E-51 | -1.69E-46 |
| MS.gene051525 | MS.gene77025 | 0.830134 | 3.48E-55 | -1.69E-46 |
| MS.gene051838 | MS.gene77025 | 0.885214 | 9.86E-72 | -1.69E-46 |
| MS.gene051941 | MS.gene77025 | 0.815076 | 1.11E-51 | -1.69E-46 |
| MS.gene052134 | MS.gene77025 | 0.834295 | 3.26E-56 | -1.69E-46 |
| MS.gene052135 | MS.gene77025 | 0.816935 | 4.28E-52 | -1.69E-46 |
| MS.gene052136 | MS.gene77025 | 0.825563 | 4.38E-54 | -1.69E-46 |
| MS.gene052164 | MS.gene77025 | 0.813467 | 2.52E-51 | -1.69E-46 |
| MS.gene052318 | MS.gene77025 | 0.803572 | 3.27E-49 | -1.69E-46 |
| MS.gene052346 | MS.gene77025 | 0.83572 | 1.42E-56 | -1.69E-46 |
| MS.gene052414 | MS.gene77025 | 0.8209 | 5.37E-53 | -1.69E-46 |
| MS.gene052481 | MS.gene77025 | 0.813565 | 2.40E-51 | -1.69E-46 |
| MS.gene052501 | MS.gene77025 | 0.849264 | 3.62E-60 | -1.69E-46 |
| MS.gene052503 | MS.gene77025 | 0.861352 | 1.09E-63 | -1.69E-46 |
| MS.gene052505 | MS.gene77025 | 0.837108 | 6.31E-57 | -1.69E-46 |
| MS.gene052615 | MS.gene77025 | 0.853031 | 3.13E-61 | -1.69E-46 |
| MS.gene052823 | MS.gene77025 | 0.850979 | 1.20E-60 | -1.69E-46 |
| MS.gene052825 | MS.gene77025 | 0.851131 | 1.08E-60 | -1.69E-46 |
| MS.gene052870 | MS.gene77025 | 0.813362 | 2.66E-51 | -1.69E-46 |
| MS.gene052872 | MS.gene77025 | 0.824695 | 7.02E-54 | -1.69E-46 |
| MS.gene052966 | MS.gene77025 | 0.843545 | 1.31E-58 | -1.69E-46 |
| MS.gene052982 | MS.gene77025 | 0.856497 | 3.09E-62 | -1.69E-46 |
| MS.gene053203 | MS.gene77025 | 0.84993 | 2.36E-60 | -1.69E-46 |
| MS.gene053207 | MS.gene77025 | 0.847154 | 1.38E-59 | -1.69E-46 |
| MS.gene053213 | MS.gene77025 | 0.824302 | 8.69E-54 | -1.69E-46 |
| MS.gene053286 | MS.gene77025 | 0.817433 | 3.31E-52 | -1.69E-46 |
| MS.gene053436 | MS.gene77025 | 0.837987 | 3.76E-57 | -1.69E-46 |
| MS.gene053479 | MS.gene77025 | 0.834164 | 3.51E-56 | -1.69E-46 |
| MS.gene053480 | MS.gene77025 | 0.838838 | 2.27E-57 | -1.69E-46 |
| MS.gene053574 | MS.gene77025 | 0.829737 | 4.35E-55 | -1.69E-46 |
| MS.gene053824 | MS.gene77025 | 0.857684 | 1.38E-62 | -1.69E-46 |
| MS.gene053997 | MS.gene77025 | 0.803884 | 2.82E-49 | -1.69E-46 |
| MS.gene054107 | MS.gene77025 | 0.82105 | 4.96E-53 | -1.69E-46 |
| MS.gene054343 | MS.gene77025 | 0.818217 | 2.20E-52 | -1.69E-46 |
| MS.gene054344 | MS.gene77025 | 0.816028 | 6.83E-52 | -1.69E-46 |
| MS.gene054408 | MS.gene77025 | 0.822433 | 2.38E-53 | -1.69E-46 |
| MS.gene054600 | MS.gene77025 | 0.849328 | 3.47E-60 | -1.69E-46 |
| MS.gene05470 | MS.gene77025 | 0.822495 | 2.30E-53 | -1.69E-46 |
| MS.gene054927 | MS.gene77025 | 0.83939 | 1.63E-57 | -1.69E-46 |
| MS.gene05494 | MS.gene77025 | 0.814223 | 1.72E-51 | -1.69E-46 |
| MS.gene055206 | MS.gene77025 | 0.830163 | 3.43E-55 | -1.69E-46 |
| MS.gene055289 | MS.gene77025 | 0.842775 | 2.10E-58 | -1.69E-46 |
| MS.gene055362 | MS.gene77025 | 0.830945 | 2.21E-55 | -1.69E-46 |
| MS.gene055502 | MS.gene77025 | 0.817906 | 2.59E-52 | -1.69E-46 |
| MS.gene055577 | MS.gene77025 | 0.826262 | 2.99E-54 | -1.69E-46 |
| MS.gene055722 | MS.gene77025 | 0.831526 | 1.59E-55 | -1.69E-46 |
| MS.gene055741 | MS.gene77025 | 0.811359 | 7.28E-51 | -1.69E-46 |
| MS.gene055744 | MS.gene77025 | 0.805162 | 1.52E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene77025 | 0.852133 | 5.64E-61 | -1.69E-46 |
| MS.gene055941 | MS.gene77025 | 0.812371 | 4.39E-51 | -1.69E-46 |
| MS.gene056166 | MS.gene77025 | 0.836148 | 1.11E-56 | -1.69E-46 |
| MS.gene056446 | MS.gene77025 | 0.811402 | 7.13E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene77025 | 0.834628 | 2.68E-56 | -1.69E-46 |
| MS.gene056510 | MS.gene77025 | 0.802319 | 5.94E-49 | -1.69E-46 |
| MS.gene056877 | MS.gene77025 | 0.858107 | 1.03E-62 | -1.69E-46 |
| MS.gene057048 | MS.gene77025 | 0.807582 | 4.71E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene77025 | 0.827007 | 1.98E-54 | -1.69E-46 |
| MS.gene057086 | MS.gene77025 | 0.801814 | 7.54E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene77025 | 0.82043 | 6.89E-53 | -1.69E-46 |
| MS.gene057196 | MS.gene77025 | 0.84184 | 3.72E-58 | -1.69E-46 |
| MS.gene057331 | MS.gene77025 | 0.831656 | 1.48E-55 | -1.69E-46 |
| MS.gene057332 | MS.gene77025 | 0.829585 | 4.74E-55 | -1.69E-46 |
| MS.gene057416 | MS.gene77025 | 0.813893 | 2.03E-51 | -1.69E-46 |
| MS.gene057658 | MS.gene77025 | 0.83901 | 2.05E-57 | -1.69E-46 |
| MS.gene057856 | MS.gene77025 | 0.829813 | 4.17E-55 | -1.69E-46 |
| MS.gene058338 | MS.gene77025 | 0.829012 | 6.53E-55 | -1.69E-46 |
| MS.gene05837 | MS.gene77025 | 0.847928 | 8.48E-60 | -1.69E-46 |
| MS.gene058418 | MS.gene77025 | -0.801971 | 7.00E-49 | -1.69E-46 |
| MS.gene058419 | MS.gene77025 | 0.816151 | 6.41E-52 | -1.69E-46 |
| MS.gene058444 | MS.gene77025 | 0.848458 | 6.05E-60 | -1.69E-46 |
| MS.gene058544 | MS.gene77025 | 0.857305 | 1.79E-62 | -1.69E-46 |
| MS.gene058700 | MS.gene77025 | 0.821466 | 3.98E-53 | -1.69E-46 |
| MS.gene058773 | MS.gene77025 | 0.80497 | 1.67E-49 | -1.69E-46 |
| MS.gene058838 | MS.gene77025 | 0.863068 | 3.24E-64 | -1.69E-46 |
| MS.gene059022 | MS.gene77025 | 0.82152 | 3.86E-53 | -1.69E-46 |
| MS.gene059023 | MS.gene77025 | 0.873337 | 1.59E-67 | -1.69E-46 |
| MS.gene059129 | MS.gene77025 | 0.815971 | 7.03E-52 | -1.69E-46 |
| MS.gene05914 | MS.gene77025 | 0.80791 | 4.01E-50 | -1.69E-46 |
| MS.gene059303 | MS.gene77025 | 0.816442 | 5.52E-52 | -1.69E-46 |
| MS.gene059512 | MS.gene77025 | 0.809637 | 1.71E-50 | -1.69E-46 |
| MS.gene059667 | MS.gene77025 | 0.814008 | 1.92E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene77025 | 0.803974 | 2.70E-49 | -1.69E-46 |
| MS.gene059718 | MS.gene77025 | 0.829506 | 4.96E-55 | -1.69E-46 |
| MS.gene059756 | MS.gene77025 | 0.803304 | 3.72E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene77025 | 0.824235 | 9.01E-54 | -1.69E-46 |
| MS.gene060087 | MS.gene77025 | -0.801394 | 9.19E-49 | -1.69E-46 |
| MS.gene060469 | MS.gene77025 | 0.819651 | 1.04E-52 | -1.69E-46 |
| MS.gene060485 | MS.gene77025 | 0.8389 | 2.18E-57 | -1.69E-46 |
| MS.gene060762 | MS.gene77025 | 0.822883 | 1.87E-53 | -1.69E-46 |
| MS.gene06077 | MS.gene77025 | 0.830801 | 2.39E-55 | -1.69E-46 |
| MS.gene060835 | MS.gene77025 | 0.841372 | 4.94E-58 | -1.69E-46 |
| MS.gene06092 | MS.gene77025 | 0.847587 | 1.05E-59 | -1.69E-46 |
| MS.gene060982 | MS.gene77025 | 0.821898 | 3.16E-53 | -1.69E-46 |
| MS.gene061150 | MS.gene77025 | 0.822666 | 2.10E-53 | -1.69E-46 |
| MS.gene061154 | MS.gene77025 | 0.802823 | 4.67E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene77025 | MS.gene48835 | PPI |
| MS.gene76713 | MS.gene77025 | PPI |
| MS.gene77025 | MS.gene76714 | PPI |
| MS.gene77025 | MS.gene53305 | PPI |
| MS.gene77025 | MS.gene48797 | PPI |
| MS.gene77025 | MS.gene76713 | PPI |
| MS.gene77025 | MS.gene76747 | PPI |
| MS.gene77025 | MS.gene76705 | PPI |
| MS.gene77025 | MS.gene82219 | PPI |
| MS.gene77025 | MS.gene48831 | PPI |
| MS.gene77025 | MS.gene53311 | PPI |
| MS.gene77025 | MS.gene82253 | PPI |
| MS.gene77025 | MS.gene76710 | PPI |
| MS.gene53312 | MS.gene77025 | PPI |
| MS.gene77025 | MS.gene77022 | PPI |
| MS.gene77025 | MS.gene82221 | PPI |
| MS.gene77025 | MS.gene82256 | PPI |
| MS.gene77025 | MS.gene48796 | PPI |
| MS.gene77025 | MS.gene48828 | PPI |
| MS.gene77025 | MS.gene48829 | PPI |
| MS.gene77025 | MS.gene53312 | PPI |
| MS.gene77025 | MS.gene76745 | PPI |
| MS.gene77025 | MS.gene82222 | PPI |
| MS.gene77025 | MS.gene53309 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77025.t1 | MTR_4g029350 | 93.162 | 117 | 8 | 0 | 1 | 117 | 1 | 117 | 7.91e-75 | 217 |
| MS.gene77025.t1 | MTR_4g029390 | 83.761 | 117 | 19 | 0 | 1 | 117 | 1 | 117 | 3.27e-66 | 196 |
| MS.gene77025.t1 | MTR_4g428370 | 64.103 | 117 | 39 | 2 | 1 | 116 | 58 | 172 | 1.86e-44 | 142 |
| MS.gene77025.t1 | MTR_4g027840 | 61.538 | 117 | 42 | 2 | 1 | 116 | 1 | 115 | 1.48e-43 | 139 |
| MS.gene77025.t1 | MTR_4g027800 | 60.684 | 117 | 43 | 2 | 1 | 116 | 1 | 115 | 2.31e-43 | 138 |
| MS.gene77025.t1 | MTR_4g029210 | 50.000 | 112 | 55 | 1 | 1 | 112 | 1 | 111 | 2.80e-31 | 107 |
| MS.gene77025.t1 | MTR_4g028180 | 45.299 | 117 | 55 | 4 | 8 | 116 | 11 | 126 | 1.45e-25 | 93.2 |
| MS.gene77025.t1 | MTR_4g028190 | 39.831 | 118 | 70 | 1 | 1 | 117 | 5 | 122 | 2.53e-24 | 90.1 |
| MS.gene77025.t1 | MTR_7g072930 | 40.187 | 107 | 61 | 2 | 10 | 114 | 12 | 117 | 2.54e-21 | 83.2 |
| MS.gene77025.t1 | MTR_3g467100 | 34.821 | 112 | 73 | 0 | 5 | 116 | 3 | 114 | 5.57e-20 | 78.6 |
| MS.gene77025.t1 | MTR_7g072830 | 35.043 | 117 | 69 | 3 | 7 | 116 | 4 | 120 | 3.73e-19 | 76.6 |
| MS.gene77025.t1 | MTR_7g073150 | 39.655 | 116 | 66 | 3 | 2 | 114 | 3 | 117 | 5.57e-19 | 76.6 |
| MS.gene77025.t1 | MTR_2g062600 | 34.906 | 106 | 67 | 2 | 11 | 115 | 10 | 114 | 4.10e-18 | 73.9 |
| MS.gene77025.t1 | MTR_7g073170 | 36.937 | 111 | 64 | 5 | 10 | 117 | 12 | 119 | 9.17e-18 | 73.2 |
| MS.gene77025.t1 | MTR_3g056640 | 33.621 | 116 | 74 | 2 | 3 | 116 | 8 | 122 | 1.40e-17 | 72.8 |
| MS.gene77025.t1 | MTR_7g072730 | 34.188 | 117 | 72 | 4 | 2 | 114 | 3 | 118 | 3.48e-17 | 72.8 |
| MS.gene77025.t1 | MTR_3g046515 | 31.579 | 114 | 75 | 2 | 3 | 114 | 8 | 120 | 3.09e-16 | 69.3 |
| MS.gene77025.t1 | MTR_4g028250 | 33.898 | 118 | 76 | 2 | 1 | 116 | 1 | 118 | 4.44e-16 | 68.9 |
| MS.gene77025.t1 | MTR_3g046350 | 34.513 | 113 | 68 | 3 | 4 | 114 | 9 | 117 | 5.09e-16 | 68.6 |
| MS.gene77025.t1 | MTR_3g046530 | 31.034 | 116 | 77 | 2 | 3 | 116 | 8 | 122 | 6.97e-16 | 68.6 |
| MS.gene77025.t1 | MTR_4g028310 | 35.833 | 120 | 71 | 3 | 1 | 116 | 1 | 118 | 7.86e-16 | 68.6 |
| MS.gene77025.t1 | MTR_3g046540 | 31.034 | 116 | 77 | 2 | 3 | 116 | 8 | 122 | 1.13e-15 | 67.8 |
| MS.gene77025.t1 | MTR_3g464130 | 34.951 | 103 | 64 | 2 | 13 | 113 | 5 | 106 | 4.24e-15 | 65.9 |
| MS.gene77025.t1 | MTR_4g028360 | 32.456 | 114 | 73 | 3 | 4 | 115 | 1 | 112 | 2.35e-14 | 64.7 |
| MS.gene77025.t1 | MTR_7g451500 | 33.684 | 95 | 62 | 1 | 22 | 115 | 4 | 98 | 2.67e-14 | 63.9 |
| MS.gene77025.t1 | MTR_3g464110 | 40.964 | 83 | 46 | 2 | 34 | 114 | 92 | 173 | 5.52e-14 | 64.7 |
| MS.gene77025.t1 | MTR_7g073120 | 33.898 | 118 | 73 | 3 | 1 | 113 | 1 | 118 | 6.43e-14 | 64.3 |
| MS.gene77025.t1 | MTR_7g072993 | 35.345 | 116 | 71 | 2 | 2 | 113 | 3 | 118 | 2.11e-13 | 62.4 |
| MS.gene77025.t1 | MTR_7g073130 | 32.456 | 114 | 74 | 3 | 2 | 113 | 3 | 115 | 8.86e-13 | 60.5 |
| MS.gene77025.t1 | MTR_7g072900 | 38.053 | 113 | 64 | 5 | 4 | 113 | 7 | 116 | 3.71e-12 | 58.9 |
| MS.gene77025.t1 | MTR_1g040335 | 38.333 | 120 | 67 | 5 | 1 | 115 | 1 | 118 | 4.71e-12 | 58.5 |
| MS.gene77025.t1 | MTR_7g072960 | 34.783 | 115 | 53 | 4 | 4 | 114 | 7 | 103 | 5.75e-12 | 58.5 |
| MS.gene77025.t1 | MTR_7g072980 | 31.304 | 115 | 76 | 2 | 2 | 113 | 3 | 117 | 2.28e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77025.t1 | AT5G59320 | 50.000 | 110 | 52 | 1 | 7 | 116 | 9 | 115 | 4.73e-29 | 102 |
| MS.gene77025.t1 | AT5G59310 | 52.475 | 101 | 45 | 1 | 16 | 116 | 15 | 112 | 1.98e-27 | 97.8 |
| MS.gene77025.t1 | AT3G51590 | 45.872 | 109 | 53 | 3 | 12 | 115 | 8 | 115 | 1.31e-24 | 90.9 |
| MS.gene77025.t1 | AT2G38540 | 44.538 | 119 | 62 | 4 | 1 | 116 | 1 | 118 | 2.13e-23 | 87.8 |
| MS.gene77025.t1 | AT4G33355 | 44.348 | 115 | 57 | 1 | 1 | 115 | 11 | 118 | 5.77e-23 | 86.7 |
| MS.gene77025.t1 | AT4G33355 | 43.363 | 113 | 57 | 1 | 1 | 113 | 11 | 116 | 9.23e-22 | 83.6 |
| MS.gene77025.t1 | AT5G01870 | 39.655 | 116 | 66 | 3 | 4 | 116 | 2 | 116 | 1.08e-21 | 83.2 |
| MS.gene77025.t1 | AT3G51600 | 40.678 | 118 | 68 | 2 | 1 | 116 | 1 | 118 | 3.38e-21 | 82.0 |
| MS.gene77025.t1 | AT2G15050 | 40.179 | 112 | 60 | 4 | 12 | 116 | 11 | 122 | 9.86e-21 | 80.9 |
| MS.gene77025.t1 | AT2G38530 | 44.348 | 115 | 57 | 3 | 9 | 116 | 4 | 118 | 1.31e-20 | 80.5 |
| MS.gene77025.t1 | AT2G15050 | 40.909 | 110 | 58 | 4 | 12 | 114 | 11 | 120 | 8.95e-20 | 78.6 |
| MS.gene77025.t1 | AT2G15050 | 41.748 | 103 | 55 | 3 | 12 | 109 | 11 | 113 | 2.83e-19 | 77.0 |
| MS.gene77025.t1 | AT3G08770 | 43.158 | 95 | 50 | 3 | 25 | 116 | 20 | 113 | 7.29e-19 | 75.9 |
| MS.gene77025.t1 | AT3G08770 | 43.011 | 93 | 49 | 3 | 25 | 114 | 20 | 111 | 8.11e-18 | 73.2 |
| MS.gene77025.t1 | AT2G18370 | 31.034 | 116 | 78 | 2 | 1 | 115 | 1 | 115 | 6.58e-15 | 65.9 |
Find 34 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTAGAAGTGCTAAACTTAT+AGG | 0.238471 | 4.3:-70399224 | None:intergenic |
| GCACTTCTACCAACTGTAAT+AGG | 0.262122 | 4.3:+70399236 | MS.gene77025:CDS |
| GATAAGTAATGCATGGAATA+AGG | 0.317466 | 4.3:-70399018 | None:intergenic |
| CATGTGGCTGCCTCTGCTAT+TGG | 0.339290 | 4.3:-70398977 | None:intergenic |
| CCATCTCCACAGTGCTGTGC+TGG | 0.355566 | 4.3:+70399058 | MS.gene77025:CDS |
| CCATGCATTACTTATCTTCA+AGG | 0.366651 | 4.3:+70399025 | MS.gene77025:CDS |
| TTGCAAGCAGCCTTGCGAGC+CGG | 0.406399 | 4.3:-70399115 | None:intergenic |
| CAACACTGGCCCGGCTCGCA+AGG | 0.417244 | 4.3:+70399105 | MS.gene77025:CDS |
| CACAGTGCTGTGCTGGAGTA+AGG | 0.420837 | 4.3:+70399065 | MS.gene77025:CDS |
| TGCAAGCAGCCTTGCGAGCC+GGG | 0.430405 | 4.3:-70399114 | None:intergenic |
| GTAGAAGTGCTAAACTTATA+GGG | 0.434237 | 4.3:-70399223 | None:intergenic |
| TGCAATTATGTGCATGGTGG+TGG | 0.439721 | 4.3:+70398946 | MS.gene77025:CDS |
| CTTGCGAGCCGGGCCAGTGT+TGG | 0.451576 | 4.3:-70399104 | None:intergenic |
| CGCCGCCGCCAACACTGGCC+CGG | 0.466490 | 4.3:+70399096 | MS.gene77025:CDS |
| AGCCGGGCCAGTGTTGGCGG+CGG | 0.471641 | 4.3:-70399098 | None:intergenic |
| ACTTATCTTCAAGGAGGTGC+TGG | 0.481218 | 4.3:+70399034 | MS.gene77025:CDS |
| GCGAGCCGGGCCAGTGTTGG+CGG | 0.487316 | 4.3:-70399101 | None:intergenic |
| GCTGCTCTCCCTGGCAAATG+TGG | 0.495611 | 4.3:+70399190 | MS.gene77025:CDS |
| CTTAACGCCGCCGCCAACAC+TGG | 0.495662 | 4.3:+70399091 | MS.gene77025:CDS |
| CCAGCACAGCACTGTGGAGA+TGG | 0.501400 | 4.3:-70399058 | None:intergenic |
| AACCAAGCTGCTGCTCTCCC+TGG | 0.514408 | 4.3:+70399181 | MS.gene77025:CDS |
| TGCCAGGGAGAGCAGCAGCT+TGG | 0.525683 | 4.3:-70399183 | None:intergenic |
| GTTGTTATTCAAACGAGCAA+TGG | 0.551011 | 4.3:-70399161 | None:intergenic |
| AACTGCTTGAAAAGTGCTGC+TGG | 0.566956 | 4.3:+70399136 | MS.gene77025:CDS |
| ATCAACTAGTTGACCGCATG+TGG | 0.580514 | 4.3:-70398993 | None:intergenic |
| CCTTGAAGATAAGTAATGCA+TGG | 0.589008 | 4.3:-70399025 | None:intergenic |
| TGTGGTTGCAATTATGTGCA+TGG | 0.608819 | 4.3:+70398940 | MS.gene77025:CDS |
| TAAAACATACCTATTACAGT+TGG | 0.611499 | 4.3:-70399245 | None:intergenic |
| GGTTAGTGCACCAATAGCAG+AGG | 0.668844 | 4.3:+70398967 | MS.gene77025:CDS |
| TGCATTACTTATCTTCAAGG+AGG | 0.675764 | 4.3:+70399028 | MS.gene77025:CDS |
| AAGCTTGAGAGTTACAATTG+TGG | 0.688534 | 4.3:+70398922 | MS.gene77025:CDS |
| CTTACTCCAGCACAGCACTG+TGG | 0.718135 | 4.3:-70399064 | None:intergenic |
| GGTTGCAATTATGTGCATGG+TGG | 0.720687 | 4.3:+70398943 | MS.gene77025:CDS |
| ATAGCAGAGGCAGCCACATG+CGG | 0.724717 | 4.3:+70398980 | MS.gene77025:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGAGTTATATATATAATTA+GGG | - | chr4.3:70399280-70399299 | None:intergenic | 10.0% |
| !! | TAAGAGTTATATATATAATT+AGG | - | chr4.3:70399281-70399300 | None:intergenic | 10.0% |
| !! | AAACAATACCTTAAAAAAAA+AGG | + | chr4.3:70399335-70399354 | MS.gene77025:intron | 15.0% |
| !! | AATTAGGTATTATTATAACA+GGG | + | chr4.3:70399580-70399599 | MS.gene77025:intron | 15.0% |
| !! | ATTGAAATTGAAATGATAAT+TGG | + | chr4.3:70399498-70399517 | MS.gene77025:intron | 15.0% |
| !! | TAATTAGGTATTATTATAAC+AGG | + | chr4.3:70399579-70399598 | MS.gene77025:intron | 15.0% |
| !! | TATAACTCTTAAAAATTTCT+AGG | + | chr4.3:70399290-70399309 | MS.gene77025:intron | 15.0% |
| !!! | ATATGTGTCATTTATTTTTT+TGG | + | chr4.3:70399531-70399550 | MS.gene77025:intron | 15.0% |
| !!! | TCATTTTACCTTTTTTTTTA+AGG | - | chr4.3:70399346-70399365 | None:intergenic | 15.0% |
| !! | AGCAGAAAATCAATTTATAA+TGG | + | chr4.3:70399387-70399406 | MS.gene77025:intron | 20.0% |
| !! | TATTGTTTATATTTGTTTCC+AGG | - | chr4.3:70399323-70399342 | None:intergenic | 20.0% |
| !!! | TTGAAGCTTTATAAAATAAG+TGG | + | chr4.3:70399638-70399657 | MS.gene77025:intron | 20.0% |
| ! | GAAATTGAAATGATAATTGG+AGG | + | chr4.3:70399501-70399520 | MS.gene77025:intron | 25.0% |
| ! | TAAAACATACCTATTACAGT+TGG | - | chr4.3:70399248-70399267 | None:intergenic | 25.0% |
| !! | TAGCATTACTTTCATTTTGA+TGG | - | chr4.3:70399700-70399719 | None:intergenic | 25.0% |
| !! | TTTTTGTCATCATCTTAGTT+TGG | - | chr4.3:70399467-70399486 | None:intergenic | 25.0% |
| !!! | ATGATTGACTTATGCAATTT+TGG | + | chr4.3:70399670-70399689 | MS.gene77025:intron | 25.0% |
| !!! | CAGGGTTTTTTTTAACTAAA+AGG | + | chr4.3:70399598-70399617 | MS.gene77025:intron | 25.0% |
| !!! | GTACATGTGTCATTTTAATT+AGG | + | chr4.3:70399564-70399583 | MS.gene77025:intron | 25.0% |
| AAAGAAACATCTATATGCTC+AGG | - | chr4.3:70399836-70399855 | None:intergenic | 30.0% | |
| ATTGAAATGATAATTGGAGG+AGG | + | chr4.3:70399504-70399523 | MS.gene77025:intron | 30.0% | |
| GATAAGTAATGCATGGAATA+AGG | - | chr4.3:70399021-70399040 | None:intergenic | 30.0% | |
| !! | AAATTTCTAGGATTCAAACC+TGG | + | chr4.3:70399302-70399321 | MS.gene77025:intron | 30.0% |
| !! | GTAGAAGTGCTAAACTTATA+GGG | - | chr4.3:70399226-70399245 | None:intergenic | 30.0% |
| AAACATCTATATGCTCAGGA+AGG | - | chr4.3:70399832-70399851 | None:intergenic | 35.0% | |
| AACATCTATATGCTCAGGAA+GGG | - | chr4.3:70399831-70399850 | None:intergenic | 35.0% | |
| AAGCTTGAGAGTTACAATTG+TGG | + | chr4.3:70398922-70398941 | MS.gene77025:CDS | 35.0% | |
| CCATGCATTACTTATCTTCA+AGG | + | chr4.3:70399025-70399044 | MS.gene77025:CDS | 35.0% | |
| CCTTGAAGATAAGTAATGCA+TGG | - | chr4.3:70399028-70399047 | None:intergenic | 35.0% | |
| TGCATTACTTATCTTCAAGG+AGG | + | chr4.3:70399028-70399047 | MS.gene77025:CDS | 35.0% | |
| ! | GTTGTTATTCAAACGAGCAA+TGG | - | chr4.3:70399164-70399183 | None:intergenic | 35.0% |
| ! | TTGTCATCATCTTAGTTTGG+AGG | - | chr4.3:70399464-70399483 | None:intergenic | 35.0% |
| ! | TTTTAACACCACATTTGCCA+GGG | - | chr4.3:70399201-70399220 | None:intergenic | 35.0% |
| !! | ATTTTAACACCACATTTGCC+AGG | - | chr4.3:70399202-70399221 | None:intergenic | 35.0% |
| !! | GGTAGAAGTGCTAAACTTAT+AGG | - | chr4.3:70399227-70399246 | None:intergenic | 35.0% |
| GCACTTCTACCAACTGTAAT+AGG | + | chr4.3:70399236-70399255 | MS.gene77025:CDS | 40.0% | |
| TGTGGTTGCAATTATGTGCA+TGG | + | chr4.3:70398940-70398959 | MS.gene77025:CDS | 40.0% | |
| ACTTGATGCTGCAATACAGC+AGG | - | chr4.3:70399916-70399935 | None:intergenic | 45.0% | |
| ATCAACTAGTTGACCGCATG+TGG | - | chr4.3:70398996-70399015 | None:intergenic | 45.0% | |
| GGTTGCAATTATGTGCATGG+TGG | + | chr4.3:70398943-70398962 | MS.gene77025:CDS | 45.0% | |
| TGCAATTATGTGCATGGTGG+TGG | + | chr4.3:70398946-70398965 | MS.gene77025:CDS | 45.0% | |
| ! | ACTTATCTTCAAGGAGGTGC+TGG | + | chr4.3:70399034-70399053 | MS.gene77025:CDS | 45.0% |
| !! | AACTGCTTGAAAAGTGCTGC+TGG | + | chr4.3:70399136-70399155 | MS.gene77025:CDS | 45.0% |
| GGTTAGTGCACCAATAGCAG+AGG | + | chr4.3:70398967-70398986 | MS.gene77025:CDS | 50.0% | |
| ATAGCAGAGGCAGCCACATG+CGG | + | chr4.3:70398980-70398999 | MS.gene77025:CDS | 55.0% | |
| CACAGTGCTGTGCTGGAGTA+AGG | + | chr4.3:70399065-70399084 | MS.gene77025:CDS | 55.0% | |
| CATGTGGCTGCCTCTGCTAT+TGG | - | chr4.3:70398980-70398999 | None:intergenic | 55.0% | |
| CTTACTCCAGCACAGCACTG+TGG | - | chr4.3:70399067-70399086 | None:intergenic | 55.0% | |
| AACCAAGCTGCTGCTCTCCC+TGG | + | chr4.3:70399181-70399200 | MS.gene77025:CDS | 60.0% | |
| CCAGCACAGCACTGTGGAGA+TGG | - | chr4.3:70399061-70399080 | None:intergenic | 60.0% | |
| CCATCTCCACAGTGCTGTGC+TGG | + | chr4.3:70399058-70399077 | MS.gene77025:CDS | 60.0% | |
| GCTGCTCTCCCTGGCAAATG+TGG | + | chr4.3:70399190-70399209 | MS.gene77025:CDS | 60.0% | |
| TTGCAAGCAGCCTTGCGAGC+CGG | - | chr4.3:70399118-70399137 | None:intergenic | 60.0% | |
| CTTAACGCCGCCGCCAACAC+TGG | + | chr4.3:70399091-70399110 | MS.gene77025:CDS | 65.0% | |
| TGCAAGCAGCCTTGCGAGCC+GGG | - | chr4.3:70399117-70399136 | None:intergenic | 65.0% | |
| TGCCAGGGAGAGCAGCAGCT+TGG | - | chr4.3:70399186-70399205 | None:intergenic | 65.0% | |
| CTTGCGAGCCGGGCCAGTGT+TGG | - | chr4.3:70399107-70399126 | None:intergenic | 70.0% | |
| ! | CAACACTGGCCCGGCTCGCA+AGG | + | chr4.3:70399105-70399124 | MS.gene77025:CDS | 70.0% |
| ! | GCGAGCCGGGCCAGTGTTGG+CGG | - | chr4.3:70399104-70399123 | None:intergenic | 75.0% |
| !! | AGCCGGGCCAGTGTTGGCGG+CGG | - | chr4.3:70399101-70399120 | None:intergenic | 75.0% |
| ! | CGCCGCCGCCAACACTGGCC+CGG | + | chr4.3:70399096-70399115 | MS.gene77025:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 70398917 | 70399940 | 70398917 | ID=MS.gene77025 |
| chr4.3 | mRNA | 70398917 | 70399940 | 70398917 | ID=MS.gene77025.t1;Parent=MS.gene77025 |
| chr4.3 | exon | 70398917 | 70399257 | 70398917 | ID=MS.gene77025.t1.exon1;Parent=MS.gene77025.t1 |
| chr4.3 | CDS | 70398917 | 70399257 | 70398917 | ID=cds.MS.gene77025.t1;Parent=MS.gene77025.t1 |
| chr4.3 | exon | 70399928 | 70399940 | 70399928 | ID=MS.gene77025.t1.exon2;Parent=MS.gene77025.t1 |
| chr4.3 | CDS | 70399928 | 70399940 | 70399928 | ID=cds.MS.gene77025.t1;Parent=MS.gene77025.t1 |
| Gene Sequence |
| Protein sequence |
