AlfalfaGEDB | MS.gene82222

M. sativa cultivar XinJiangDaYe / MS.gene82222

KEGG: -
GO: GO:0003674 GO:0005488 GO:0006810 GO:0006869 GO:0008150 GO:0008289 GO:0051179 GO:0051234 GO:0071702
PFAM: PF00234.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82222.t1	XP_003605266.1	87.6	113	14	0	1	113	1	113	1.60E-46	195.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82222.t1	A0AT31	76.6	111	26	0	3	113	4	114	1.5e-40	166.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82222.t1	G7JI87	87.6	113	14	0	1	113	1	113	1.2e-46	195.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049260	MS.gene82222	0.827667	1.38E-54	-1.69E-46
MS.gene049758	MS.gene82222	0.843634	1.24E-58	-1.69E-46
MS.gene050128	MS.gene82222	0.839099	1.94E-57	-1.69E-46
MS.gene050301	MS.gene82222	0.807431	5.07E-50	-1.69E-46
MS.gene050406	MS.gene82222	0.805001	1.65E-49	-1.69E-46
MS.gene050642	MS.gene82222	0.812258	4.64E-51	-1.69E-46
MS.gene051983	MS.gene82222	0.810097	1.37E-50	-1.69E-46
MS.gene052077	MS.gene82222	0.812747	3.63E-51	-1.69E-46
MS.gene052178	MS.gene82222	0.841964	3.45E-58	-1.69E-46
MS.gene052340	MS.gene82222	0.828831	7.22E-55	-1.69E-46
MS.gene053073	MS.gene82222	0.8157	8.08E-52	-1.69E-46
MS.gene053209	MS.gene82222	0.834836	2.38E-56	-1.69E-46
MS.gene053557	MS.gene82222	0.839444	1.58E-57	-1.69E-46
MS.gene053836	MS.gene82222	0.804413	2.19E-49	-1.69E-46
MS.gene053928	MS.gene82222	0.824942	6.14E-54	-1.69E-46
MS.gene055604	MS.gene82222	0.801822	7.51E-49	-1.69E-46
MS.gene056612	MS.gene82222	0.809555	1.79E-50	-1.69E-46
MS.gene057396	MS.gene82222	0.801672	8.06E-49	-1.69E-46
MS.gene057690	MS.gene82222	0.81339	2.62E-51	-1.69E-46
MS.gene057752	MS.gene82222	0.818313	2.09E-52	-1.69E-46
MS.gene057754	MS.gene82222	0.803507	3.37E-49	-1.69E-46
MS.gene058002	MS.gene82222	0.816234	6.14E-52	-1.69E-46
MS.gene05811	MS.gene82222	0.809728	1.64E-50	-1.69E-46
MS.gene058142	MS.gene82222	0.811327	7.40E-51	-1.69E-46
MS.gene058276	MS.gene82222	0.815371	9.57E-52	-1.69E-46
MS.gene059518	MS.gene82222	0.819875	9.23E-53	-1.69E-46
MS.gene05984	MS.gene82222	0.800167	1.64E-48	-1.69E-46
MS.gene059894	MS.gene82222	0.848433	6.15E-60	-1.69E-46
MS.gene059899	MS.gene82222	0.809969	1.45E-50	-1.69E-46
MS.gene06037	MS.gene82222	0.812347	4.44E-51	-1.69E-46
MS.gene061049	MS.gene82222	0.841607	4.29E-58	-1.69E-46
MS.gene061112	MS.gene82222	0.80822	3.45E-50	-1.69E-46
MS.gene061265	MS.gene82222	0.810081	1.38E-50	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene76705	MS.gene82222	PPI
MS.gene76710	MS.gene82222	PPI
MS.gene53309	MS.gene82222	PPI
MS.gene48831	MS.gene82222	PPI
MS.gene82222	MS.gene82219	PPI
MS.gene53311	MS.gene82222	PPI
MS.gene48796	MS.gene82222	PPI
MS.gene76713	MS.gene82222	PPI
MS.gene76747	MS.gene82222	PPI
MS.gene53305	MS.gene82222	PPI
MS.gene82256	MS.gene82222	PPI
MS.gene48835	MS.gene82222	PPI
MS.gene82221	MS.gene82222	PPI
MS.gene48829	MS.gene82222	PPI
MS.gene82253	MS.gene82222	PPI
MS.gene82222	MS.gene53309	PPI
MS.gene76714	MS.gene82222	PPI
MS.gene82222	MS.gene76714	PPI
MS.gene48797	MS.gene82222	PPI
MS.gene77025	MS.gene82222	PPI
MS.gene76745	MS.gene82222	PPI
MS.gene53312	MS.gene82222	PPI
MS.gene77022	MS.gene82222	PPI
MS.gene48828	MS.gene82222	PPI
MS.gene82219	MS.gene82222	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82222.t1	MTR_4g027800	87.611	113	14	0	1	113	1	113	2.62e-66	196
MS.gene82222.t1	MTR_4g027840	87.611	113	14	0	1	113	1	113	1.20e-65	195
MS.gene82222.t1	MTR_4g428370	82.301	113	20	0	1	113	58	170	3.88e-63	190
MS.gene82222.t1	MTR_4g029390	62.712	118	41	2	1	116	1	117	1.63e-45	143
MS.gene82222.t1	MTR_4g029350	62.712	118	41	2	1	116	1	117	1.69e-44	140
MS.gene82222.t1	MTR_7g072930	49.573	117	56	2	4	118	7	122	1.55e-34	117
MS.gene82222.t1	MTR_4g029210	50.893	112	55	0	1	112	1	112	3.45e-33	112
MS.gene82222.t1	MTR_4g028190	47.458	118	60	2	1	116	5	122	9.33e-32	108
MS.gene82222.t1	MTR_7g073150	44.444	117	62	2	4	118	7	122	2.27e-29	103
MS.gene82222.t1	MTR_7g072730	40.171	117	68	2	4	118	7	123	1.11e-27	99.8
MS.gene82222.t1	MTR_7g073170	42.609	115	62	3	4	116	7	119	1.13e-26	95.9
MS.gene82222.t1	MTR_7g072830	40.000	110	64	1	4	111	7	116	5.24e-26	94.4
MS.gene82222.t1	MTR_4g028310	41.739	115	64	1	1	112	1	115	6.50e-25	92.0
MS.gene82222.t1	MTR_4g028180	42.982	114	58	3	5	111	9	122	2.29e-24	90.5
MS.gene82222.t1	MTR_2g062600	42.478	113	63	2	1	112	1	112	1.15e-23	88.2
MS.gene82222.t1	MTR_7g072960	42.857	112	48	3	9	118	11	108	1.36e-23	88.6
MS.gene82222.t1	MTR_7g073120	37.607	117	71	1	1	115	5	121	1.59e-23	89.0
MS.gene82222.t1	MTR_7g072993	41.739	115	64	1	4	115	7	121	1.57e-22	85.9
MS.gene82222.t1	MTR_7g072900	43.243	111	60	2	4	112	7	116	1.13e-21	83.2
MS.gene82222.t1	MTR_4g028250	42.105	114	63	2	1	111	1	114	6.34e-21	81.3
MS.gene82222.t1	MTR_7g073130	37.931	116	70	2	4	118	7	121	3.77e-20	79.7
MS.gene82222.t1	MTR_7g072980	36.842	114	70	1	4	115	7	120	6.32e-20	79.3
MS.gene82222.t1	MTR_7g073100	38.793	116	64	2	4	115	7	119	1.31e-18	76.3
MS.gene82222.t1	MTR_3g467100	37.037	108	67	1	5	111	3	110	2.43e-18	74.7
MS.gene82222.t1	MTR_7g451500	38.202	89	54	1	24	111	7	95	2.84e-17	71.2
MS.gene82222.t1	MTR_1g040335	42.727	110	60	2	5	112	8	116	2.89e-17	72.0
MS.gene82222.t1	MTR_4g028360	35.088	114	71	3	4	115	1	113	2.68e-16	69.7
MS.gene82222.t1	MTR_7g072810	40.870	115	58	5	4	115	7	114	3.30e-16	69.3
MS.gene82222.t1	MTR_7g073060	36.364	110	69	1	4	112	7	116	7.88e-16	68.2
MS.gene82222.t1	MTR_7g073030	33.628	113	74	1	4	115	7	119	1.81e-15	68.2
MS.gene82222.t1	MTR_3g056640	36.364	110	64	3	9	113	12	120	1.07e-13	62.8
MS.gene82222.t1	MTR_3g046515	32.456	114	69	4	4	110	7	119	9.82e-13	60.5
MS.gene82222.t1	MTR_3g464130	36.449	107	60	4	11	110	1	106	2.05e-12	59.3
MS.gene82222.t1	MTR_3g046530	31.304	115	73	3	4	113	7	120	2.29e-12	59.3
MS.gene82222.t1	MTR_3g046540	32.456	114	69	4	4	110	7	119	3.80e-12	58.9
MS.gene82222.t1	MTR_7g072987	48.529	68	32	2	25	90	57	123	1.37e-11	58.2
MS.gene82222.t1	MTR_3g464110	40.244	82	44	3	33	110	92	172	5.22e-11	57.0
MS.gene82222.t1	MTR_1g040330	35.455	110	68	2	5	112	8	116	6.67e-11	55.5
MS.gene82222.t1	MTR_7g072720	29.730	111	76	2	6	114	10	120	6.72e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82222.t1	AT5G59320	60.577	104	39	1	8	111	10	111	7.35e-37	121
MS.gene82222.t1	AT2G38540	63.158	114	36	6	4	113	5	116	2.95e-36	120
MS.gene82222.t1	AT5G59310	60.194	103	38	1	11	113	11	110	5.93e-36	119
MS.gene82222.t1	AT2G38530	59.292	113	42	4	4	113	5	116	9.83e-32	108
MS.gene82222.t1	AT3G51600	53.097	113	49	4	4	113	5	116	4.49e-29	102
MS.gene82222.t1	AT2G15050	44.915	118	59	4	4	115	5	122	8.80e-28	99.0
MS.gene82222.t1	AT3G51590	46.296	108	56	2	5	111	6	112	2.14e-26	95.5
MS.gene82222.t1	AT2G15050	44.828	116	58	4	4	113	5	120	2.40e-26	95.1
MS.gene82222.t1	AT2G15050	44.954	109	56	3	4	108	5	113	1.57e-25	92.8
MS.gene82222.t1	AT4G33355	50.562	89	44	0	23	111	27	115	1.37e-24	90.5
MS.gene82222.t1	AT4G33355	50.562	89	44	0	23	111	27	115	1.53e-24	90.5
MS.gene82222.t1	AT5G01870	44.545	110	53	4	9	111	4	112	2.55e-23	87.4
MS.gene82222.t1	AT3G08770	45.263	95	48	3	22	113	18	111	8.31e-19	75.9
MS.gene82222.t1	AT3G08770	45.263	95	48	3	22	113	18	111	1.16e-18	75.5
MS.gene82222.t1	AT2G18370	37.391	115	69	3	1	113	1	114	9.83e-18	73.2
MS.gene82222.t1	AT2G15325	33.708	89	56	2	27	113	31	118	2.13e-11	57.0

Find 30 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTGCTGCTGGTGCTATTTC+TGG	0.136175	4.2:-75477345	MS.gene82222:CDS
GGCGCAGCTTCTTCTCTTCC+TGG	0.339122	4.2:-75477312	MS.gene82222:CDS
CCATGCATTGGATATCTTAG+AGG	0.354250	4.2:-75477468	MS.gene82222:CDS
GCTATTTCTGGTTTGAATAC+AGG	0.359485	4.2:-75477333	MS.gene82222:CDS
CCATCTCCAGCATGCTGTGC+TGG	0.375274	4.2:-75477435	MS.gene82222:CDS
GAAGGTAGCATGTGTTGTTT+TGG	0.393365	4.2:-75477562	MS.gene82222:CDS
TTGCAAGCAGCCTGACGGTC+AGG	0.428772	4.2:+75477378	None:intergenic
CACTACCACACCTGACCGTC+AGG	0.433614	4.2:-75477388	MS.gene82222:CDS
GTGCATGATTGTTGCACCTA+TGG	0.467928	4.2:-75477535	MS.gene82222:CDS
GGTGCTCTTTCTCCATGCAT+TGG	0.474474	4.2:-75477480	MS.gene82222:CDS
CTGACGGTCAGGTGTGGTAG+TGG	0.482598	4.2:+75477389	None:intergenic
TTCACTCCAGCACAGCATGC+TGG	0.485039	4.2:+75477429	None:intergenic
AACTGCTTGAAAAGTGCTGC+TGG	0.486030	4.2:-75477357	MS.gene82222:CDS
GGATATCTTAGAGGTGGTGC+TGG	0.504129	4.2:-75477459	MS.gene82222:CDS
GTGGAGGTACTGATCTTGTA+AGG	0.508470	4.2:+75477270	None:intergenic
GTCAGGTGTGGTAGTGGCGG+CGG	0.531452	4.2:+75477395	None:intergenic
TCTTCTCTTCCTGGCAAATG+TGG	0.544086	4.2:-75477303	MS.gene82222:CDS
CCAGCACAGCATGCTGGAGA+TGG	0.550113	4.2:+75477435	None:intergenic
AACATACGTAGCACAGTTGG+TGG	0.554041	4.2:+75477251	None:intergenic
ATGTTAACACCACATTTGCC+AGG	0.555990	4.2:+75477294	None:intergenic
GAGATTGCAGCATCTGCCAT+AGG	0.577723	4.2:+75477519	None:intergenic
CCTCTAAGATATCCAATGCA+TGG	0.598915	4.2:+75477468	None:intergenic
ATAAACATACGTAGCACAGT+TGG	0.615719	4.2:+75477248	None:intergenic
AGCAGCCTGACGGTCAGGTG+TGG	0.616597	4.2:+75477383	None:intergenic
TGCATTGGATATCTTAGAGG+TGG	0.623803	4.2:-75477465	MS.gene82222:CDS
AGCAGTTGCAAGCAGCCTGA+CGG	0.624471	4.2:+75477373	None:intergenic
ACGGTCAGGTGTGGTAGTGG+CGG	0.626810	4.2:+75477392	None:intergenic
ATACGTAGCACAGTTGGTGG+AGG	0.639269	4.2:+75477254	None:intergenic
GCAGATGCTGCAATCTCATG+TGG	0.654132	4.2:-75477513	MS.gene82222:CDS
ATCTCATGTGGACAAGTGAG+TGG	0.724614	4.2:-75477501	MS.gene82222:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATAAACATACGTAGCACAGT+TGG	+	chr4.2:75477567-75477586	None:intergenic	35.0%
!!	GCTATTTCTGGTTTGAATAC+AGG	-	chr4.2:75477479-75477498	MS.gene82222:CDS	35.0%
	ATGTTAACACCACATTTGCC+AGG	+	chr4.2:75477521-75477540	None:intergenic	40.0%
	CCATGCATTGGATATCTTAG+AGG	-	chr4.2:75477344-75477363	MS.gene82222:CDS	40.0%
	TGCATTGGATATCTTAGAGG+TGG	-	chr4.2:75477347-75477366	MS.gene82222:CDS	40.0%
!!	CCTCTAAGATATCCAATGCA+TGG	+	chr4.2:75477347-75477366	None:intergenic	40.0%
!!!	GAAGGTAGCATGTGTTGTTT+TGG	-	chr4.2:75477250-75477269	MS.gene82222:CDS	40.0%
	AACATACGTAGCACAGTTGG+TGG	+	chr4.2:75477564-75477583	None:intergenic	45.0%
	ATCTCATGTGGACAAGTGAG+TGG	-	chr4.2:75477311-75477330	MS.gene82222:CDS	45.0%
	GTGCATGATTGTTGCACCTA+TGG	-	chr4.2:75477277-75477296	MS.gene82222:CDS	45.0%
	TCTTCTCTTCCTGGCAAATG+TGG	-	chr4.2:75477509-75477528	MS.gene82222:CDS	45.0%
!!	AACTGCTTGAAAAGTGCTGC+TGG	-	chr4.2:75477455-75477474	MS.gene82222:CDS	45.0%
!!	GTGGAGGTACTGATCTTGTA+AGG	+	chr4.2:75477545-75477564	None:intergenic	45.0%
	GAGATTGCAGCATCTGCCAT+AGG	+	chr4.2:75477296-75477315	None:intergenic	50.0%
	GCAGATGCTGCAATCTCATG+TGG	-	chr4.2:75477299-75477318	MS.gene82222:CDS	50.0%
	GGATATCTTAGAGGTGGTGC+TGG	-	chr4.2:75477353-75477372	MS.gene82222:CDS	50.0%
	GGTGCTCTTTCTCCATGCAT+TGG	-	chr4.2:75477332-75477351	MS.gene82222:CDS	50.0%
!!	AGTGCTGCTGGTGCTATTTC+TGG	-	chr4.2:75477467-75477486	MS.gene82222:CDS	50.0%
!!	ATACGTAGCACAGTTGGTGG+AGG	+	chr4.2:75477561-75477580	None:intergenic	50.0%
	AGCAGTTGCAAGCAGCCTGA+CGG	+	chr4.2:75477442-75477461	None:intergenic	55.0%
	TTCACTCCAGCACAGCATGC+TGG	+	chr4.2:75477386-75477405	None:intergenic	55.0%
	CACTACCACACCTGACCGTC+AGG	-	chr4.2:75477424-75477443	MS.gene82222:CDS	60.0%
	CCAGCACAGCATGCTGGAGA+TGG	+	chr4.2:75477380-75477399	None:intergenic	60.0%
	CCATCTCCAGCATGCTGTGC+TGG	-	chr4.2:75477377-75477396	MS.gene82222:CDS	60.0%
	CTGACGGTCAGGTGTGGTAG+TGG	+	chr4.2:75477426-75477445	None:intergenic	60.0%
	GGCGCAGCTTCTTCTCTTCC+TGG	-	chr4.2:75477500-75477519	MS.gene82222:CDS	60.0%
	TTGCAAGCAGCCTGACGGTC+AGG	+	chr4.2:75477437-75477456	None:intergenic	60.0%
!!	ACGGTCAGGTGTGGTAGTGG+CGG	+	chr4.2:75477423-75477442	None:intergenic	60.0%
	AGCAGCCTGACGGTCAGGTG+TGG	+	chr4.2:75477432-75477451	None:intergenic	65.0%
!!	GTCAGGTGTGGTAGTGGCGG+CGG	+	chr4.2:75477420-75477439	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	75477239	75477595	75477239	ID=MS.gene82222
chr4.2	mRNA	75477239	75477595	75477239	ID=MS.gene82222.t1;Parent=MS.gene82222
chr4.2	exon	75477239	75477595	75477239	ID=MS.gene82222.t1.exon1;Parent=MS.gene82222.t1
chr4.2	CDS	75477239	75477595	75477239	ID=cds.MS.gene82222.t1;Parent=MS.gene82222.t1

Gene Sequence	>MS.gene82222 ATGGCAAACATGAAGGTAGCATGTGTTGTTTTGGTGATGTGCATGATTGTTGCACCTATGGCAGATGCTGCAATCTCATGTGGACAAGTGAGTGGTGCTCTTTCTCCATGCATTGGATATCTTAGAGGTGGTGCTGGTCCATCTCCAGCATGCTGTGCTGGAGTGAAGAGACTTAACGCCGCCGCCACTACCACACCTGACCGTCAGGCTGCTTGCAACTGCTTGAAAAGTGCTGCTGGTGCTATTTCTGGTTTGAATACAGGCGCAGCTTCTTCTCTTCCTGGCAAATGTGGTGTTAACATTCCTTACAAGATCAGTACCTCCACCAACTGTGCTACGTATGTTTATCTAACTTAA
Protein sequence	>MS.gene82222.t1 MANMKVACVVLVMCMIVAPMADAAISCGQVSGALSPCIGYLRGGAGPSPACCAGVKRLNAAATTTPDRQAACNCLKSAAGAISGLNTGAASSLPGKCGVNIPYKISTSTNCATYVYLT