AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76713


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76713.t1 MTR_4g027840 88.696 115 13 0 1 115 1 115 1.96e-68 202
MS.gene76713.t1 MTR_4g027800 87.931 116 14 0 1 116 1 116 2.13e-68 201
MS.gene76713.t1 MTR_4g428370 84.483 116 18 0 1 116 58 173 1.13e-66 199
MS.gene76713.t1 MTR_4g029390 65.812 117 37 2 1 115 1 116 2.67e-47 148
MS.gene76713.t1 MTR_4g029350 65.812 117 37 2 1 115 1 116 2.94e-46 145
MS.gene76713.t1 MTR_4g029210 50.000 112 56 0 1 112 1 112 2.19e-32 110
MS.gene76713.t1 MTR_7g072930 49.107 112 54 2 4 113 7 117 1.45e-31 109
MS.gene76713.t1 MTR_4g028190 47.368 114 58 2 1 112 5 118 4.75e-30 104
MS.gene76713.t1 MTR_7g073150 46.552 116 57 4 2 113 3 117 2.22e-28 100
MS.gene76713.t1 MTR_7g073170 43.363 113 60 3 4 114 7 117 7.85e-27 96.3
MS.gene76713.t1 MTR_7g072830 39.474 114 67 1 4 115 7 120 2.13e-26 95.1
MS.gene76713.t1 MTR_7g072730 39.286 112 66 2 4 113 7 118 1.54e-25 94.4
MS.gene76713.t1 MTR_4g028310 40.336 119 68 1 1 116 1 119 1.91e-25 93.2
MS.gene76713.t1 MTR_4g028180 40.678 118 63 3 5 115 9 126 9.63e-25 91.3
MS.gene76713.t1 MTR_2g062600 41.739 115 65 2 1 114 1 114 5.01e-24 89.0
MS.gene76713.t1 MTR_7g073120 38.053 113 68 1 1 111 5 117 3.90e-22 85.5
MS.gene76713.t1 MTR_7g072900 43.243 111 60 2 4 112 7 116 1.58e-21 82.8
MS.gene76713.t1 MTR_7g072993 42.342 111 61 1 4 111 7 117 4.56e-21 82.0
MS.gene76713.t1 MTR_4g028250 39.831 118 68 2 1 115 1 118 8.01e-21 80.9
MS.gene76713.t1 MTR_7g072960 41.121 107 47 3 9 113 11 103 1.90e-20 80.5
MS.gene76713.t1 MTR_3g467100 36.607 112 70 1 5 115 3 114 8.59e-20 78.2
MS.gene76713.t1 MTR_7g072980 38.393 112 65 3 4 112 7 117 3.62e-18 74.7
MS.gene76713.t1 MTR_7g451500 38.043 92 56 1 24 114 7 98 5.69e-18 73.2
MS.gene76713.t1 MTR_1g040335 41.964 112 62 2 5 114 8 118 1.70e-17 72.4
MS.gene76713.t1 MTR_7g073130 38.739 111 64 4 4 112 7 115 4.12e-17 71.6
MS.gene76713.t1 MTR_7g073100 38.053 113 63 2 4 112 7 116 1.07e-16 71.2
MS.gene76713.t1 MTR_4g028360 35.398 113 70 3 4 114 1 112 1.68e-16 70.1
MS.gene76713.t1 MTR_7g073060 37.719 114 68 3 4 115 7 119 2.40e-16 69.7
MS.gene76713.t1 MTR_7g072810 39.091 110 59 3 4 111 7 110 5.04e-15 66.2
MS.gene76713.t1 MTR_7g073030 35.455 110 68 3 4 111 7 115 7.57e-14 63.9
MS.gene76713.t1 MTR_3g056640 35.398 113 65 4 9 115 12 122 7.81e-14 63.2
MS.gene76713.t1 MTR_3g046515 34.821 112 67 4 3 110 10 119 3.12e-13 61.6
MS.gene76713.t1 MTR_3g046530 31.356 118 73 4 4 115 7 122 8.76e-13 60.5
MS.gene76713.t1 MTR_3g464130 37.778 90 51 3 25 110 18 106 3.23e-12 58.5
MS.gene76713.t1 MTR_3g046540 33.043 115 67 5 4 110 7 119 6.72e-12 58.2
MS.gene76713.t1 MTR_7g072987 48.529 68 32 2 25 90 57 123 9.99e-12 58.5
MS.gene76713.t1 MTR_1g040330 38.596 114 65 4 5 115 8 119 1.22e-11 57.4
MS.gene76713.t1 MTR_3g464110 38.554 83 48 2 33 113 92 173 3.94e-11 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76713.t1 AT2G38540 62.931 116 37 6 4 115 5 118 6.42e-38 124
MS.gene76713.t1 AT5G59320 58.182 110 44 1 6 115 8 115 9.93e-38 124
MS.gene76713.t1 AT5G59310 59.091 110 40 2 6 115 8 112 5.58e-37 121
MS.gene76713.t1 AT2G38530 59.130 115 43 4 4 115 5 118 4.05e-33 112
MS.gene76713.t1 AT3G51600 52.174 115 51 4 4 115 5 118 7.27e-30 103
MS.gene76713.t1 AT2G15050 44.068 118 60 4 4 115 5 122 2.81e-27 97.4
MS.gene76713.t1 AT3G51590 45.455 110 57 3 6 114 8 115 2.83e-27 97.4
MS.gene76713.t1 AT2G15050 43.966 116 59 4 4 113 5 120 7.44e-26 94.0
MS.gene76713.t1 AT4G33355 49.462 93 47 0 23 115 27 119 2.60e-25 92.4
MS.gene76713.t1 AT2G15050 44.037 109 57 3 4 108 5 113 4.94e-25 91.7
MS.gene76713.t1 AT4G33355 50.562 89 44 0 23 111 27 115 1.16e-24 90.9
MS.gene76713.t1 AT5G01870 43.860 114 56 4 9 115 4 116 1.25e-24 90.5
MS.gene76713.t1 AT3G08770 45.361 97 49 3 22 115 18 113 5.86e-20 78.6
MS.gene76713.t1 AT3G08770 46.237 93 46 3 22 111 18 109 5.76e-19 76.3
MS.gene76713.t1 AT2G18370 36.207 116 71 3 1 114 1 115 6.16e-18 73.6
MS.gene76713.t1 AT2G15325 34.066 91 57 2 27 115 31 120 1.32e-12 60.1

Find 31 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGCTGCTGGTGCTATTTC+TGG 0.136175 4.1:+71166711 MS.gene76713:CDS
GGCGCAGCTTCTTCTCTTCC+TGG 0.339122 4.1:+71166744 MS.gene76713:CDS
CCATGCATTGGATATCTTAG+AGG 0.354250 4.1:+71166588 MS.gene76713:CDS
GCTATTTCTGGTTTGAATAC+AGG 0.359485 4.1:+71166723 MS.gene76713:CDS
CCATCTCCAGCATGCTGTGC+TGG 0.375274 4.1:+71166621 MS.gene76713:CDS
TTGCAAGCAGCCTGACGGTC+AGG 0.428772 4.1:-71166678 None:intergenic
CACTACCACACCTGACCGTC+AGG 0.433614 4.1:+71166668 MS.gene76713:CDS
GTGCATGATTGTTGCACCTA+TGG 0.467928 4.1:+71166521 MS.gene76713:CDS
GGTGCTCTTTCTCCATGCAT+TGG 0.474474 4.1:+71166576 MS.gene76713:CDS
CTGACGGTCAGGTGTGGTAG+TGG 0.482598 4.1:-71166667 None:intergenic
TTCACTCCAGCACAGCATGC+TGG 0.485039 4.1:-71166627 None:intergenic
AACTGCTTGAAAAGTGCTGC+TGG 0.486030 4.1:+71166699 MS.gene76713:CDS
ATGTTAACGCCACATTTGCC+AGG 0.486943 4.1:-71166762 None:intergenic
GGATATCTTAGAGGTGGTGC+TGG 0.504129 4.1:+71166597 MS.gene76713:CDS
GTGGAGGTACTGATCTTGTA+AGG 0.508470 4.1:-71166786 None:intergenic
GTCAGGTGTGGTAGTGGCGG+CGG 0.531452 4.1:-71166661 None:intergenic
CCAGCACAGCATGCTGGAGA+TGG 0.550113 4.1:-71166621 None:intergenic
AACATACGTAGCACAGTTGG+TGG 0.554041 4.1:-71166805 None:intergenic
TTGTTATACTTACAGCATCA+GGG 0.577540 4.1:+71167157 MS.gene76713:intron
GAGATTGCAGCATCTGCCAT+AGG 0.577723 4.1:-71166537 None:intergenic
TCTTCTCTTCCTGGCAAATG+TGG 0.581280 4.1:+71166753 MS.gene76713:CDS
AAACAATATGGCAAGCATGA+AGG 0.586081 4.1:+71166476 None:intergenic
CCTCTAAGATATCCAATGCA+TGG 0.598915 4.1:-71166588 None:intergenic
ATAAACATACGTAGCACAGT+TGG 0.615719 4.1:-71166808 None:intergenic
AGCAGCCTGACGGTCAGGTG+TGG 0.616597 4.1:-71166673 None:intergenic
TGCATTGGATATCTTAGAGG+TGG 0.623803 4.1:+71166591 MS.gene76713:CDS
AGCAGTTGCAAGCAGCCTGA+CGG 0.624471 4.1:-71166683 None:intergenic
ACGGTCAGGTGTGGTAGTGG+CGG 0.626810 4.1:-71166664 None:intergenic
ATACGTAGCACAGTTGGTGG+AGG 0.639269 4.1:-71166802 None:intergenic
GCAGATGCTGCAATCTCATG+TGG 0.654132 4.1:+71166543 MS.gene76713:CDS
ATCTCATGTGGACAAGTGAG+TGG 0.724614 4.1:+71166555 MS.gene76713:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTTTATTAAAAAAATA+TGG - chr4.1:71167029-71167048 None:intergenic 0.0%
!!! TAAATCATTCTTTTAATTTT+AGG + chr4.1:71166951-71166970 MS.gene76713:intron 10.0%
!!! TTATATTATTCAATGTTTTT+GGG + chr4.1:71167048-71167067 MS.gene76713:intron 10.0%
!!! TTTATATTATTCAATGTTTT+TGG + chr4.1:71167047-71167066 MS.gene76713:intron 10.0%
!!! AAAATTTAGTTGTTTAACTT+TGG - chr4.1:71166908-71166927 None:intergenic 15.0%
!!! TATTTTTTACCATTTGCTTA+AGG + chr4.1:71166992-71167011 MS.gene76713:intron 20.0%
! TTAAAACATCCTTAAGCAAA+TGG - chr4.1:71167004-71167023 None:intergenic 25.0%
!! TAGAAAATTAATCACTGACT+AGG + chr4.1:71167124-71167143 MS.gene76713:intron 25.0%
!!! CTACGAATCTACTTTTTTAT+TGG + chr4.1:71167092-71167111 MS.gene76713:intron 25.0%
ACTAGACTACTTGAAAAATG+AGG - chr4.1:71166860-71166879 None:intergenic 30.0%
TTGTTATACTTACAGCATCA+GGG + chr4.1:71167157-71167176 MS.gene76713:intron 30.0%
! TTACCATTTGAGATTGTTGA+GGG + chr4.1:71166928-71166947 MS.gene76713:intron 30.0%
! TTTACCATTTGAGATTGTTG+AGG + chr4.1:71166927-71166946 MS.gene76713:intron 30.0%
! TTTGTTATACTTACAGCATC+AGG + chr4.1:71167156-71167175 MS.gene76713:intron 30.0%
ATAAACATACGTAGCACAGT+TGG - chr4.1:71166811-71166830 None:intergenic 35.0%
TTACCCTCAACAATCTCAAA+TGG - chr4.1:71166934-71166953 None:intergenic 35.0%
!! GCTATTTCTGGTTTGAATAC+AGG + chr4.1:71166723-71166742 MS.gene76713:CDS 35.0%
CCATGCATTGGATATCTTAG+AGG + chr4.1:71166588-71166607 MS.gene76713:CDS 40.0%
TGCATTGGATATCTTAGAGG+TGG + chr4.1:71166591-71166610 MS.gene76713:CDS 40.0%
!! CCTCTAAGATATCCAATGCA+TGG - chr4.1:71166591-71166610 None:intergenic 40.0%
AACATACGTAGCACAGTTGG+TGG - chr4.1:71166808-71166827 None:intergenic 45.0%
ATCTCATGTGGACAAGTGAG+TGG + chr4.1:71166555-71166574 MS.gene76713:CDS 45.0%
ATGTTAACGCCACATTTGCC+AGG - chr4.1:71166765-71166784 None:intergenic 45.0%
GTGCATGATTGTTGCACCTA+TGG + chr4.1:71166521-71166540 MS.gene76713:CDS 45.0%
TCTTCTCTTCCTGGCAAATG+TGG + chr4.1:71166753-71166772 MS.gene76713:CDS 45.0%
!! AACTGCTTGAAAAGTGCTGC+TGG + chr4.1:71166699-71166718 MS.gene76713:CDS 45.0%
!! GTGGAGGTACTGATCTTGTA+AGG - chr4.1:71166789-71166808 None:intergenic 45.0%
GAGATTGCAGCATCTGCCAT+AGG - chr4.1:71166540-71166559 None:intergenic 50.0%
GCAGATGCTGCAATCTCATG+TGG + chr4.1:71166543-71166562 MS.gene76713:CDS 50.0%
GGATATCTTAGAGGTGGTGC+TGG + chr4.1:71166597-71166616 MS.gene76713:CDS 50.0%
GGTGCTCTTTCTCCATGCAT+TGG + chr4.1:71166576-71166595 MS.gene76713:CDS 50.0%
!! AGTGCTGCTGGTGCTATTTC+TGG + chr4.1:71166711-71166730 MS.gene76713:CDS 50.0%
!! ATACGTAGCACAGTTGGTGG+AGG - chr4.1:71166805-71166824 None:intergenic 50.0%
AGCAGTTGCAAGCAGCCTGA+CGG - chr4.1:71166686-71166705 None:intergenic 55.0%
TTCACTCCAGCACAGCATGC+TGG - chr4.1:71166630-71166649 None:intergenic 55.0%
CACTACCACACCTGACCGTC+AGG + chr4.1:71166668-71166687 MS.gene76713:CDS 60.0%
CCAGCACAGCATGCTGGAGA+TGG - chr4.1:71166624-71166643 None:intergenic 60.0%
CCATCTCCAGCATGCTGTGC+TGG + chr4.1:71166621-71166640 MS.gene76713:CDS 60.0%
CTGACGGTCAGGTGTGGTAG+TGG - chr4.1:71166670-71166689 None:intergenic 60.0%
GGCGCAGCTTCTTCTCTTCC+TGG + chr4.1:71166744-71166763 MS.gene76713:CDS 60.0%
TTGCAAGCAGCCTGACGGTC+AGG - chr4.1:71166681-71166700 None:intergenic 60.0%
!! ACGGTCAGGTGTGGTAGTGG+CGG - chr4.1:71166667-71166686 None:intergenic 60.0%
AGCAGCCTGACGGTCAGGTG+TGG - chr4.1:71166676-71166695 None:intergenic 65.0%
!! GTCAGGTGTGGTAGTGGCGG+CGG - chr4.1:71166664-71166683 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 71166483 71167184 71166483 ID=MS.gene76713
chr4.1 mRNA 71166483 71167184 71166483 ID=MS.gene76713.t1;Parent=MS.gene76713
chr4.1 exon 71166483 71166820 71166483 ID=MS.gene76713.t1.exon1;Parent=MS.gene76713.t1
chr4.1 CDS 71166483 71166820 71166483 ID=cds.MS.gene76713.t1;Parent=MS.gene76713.t1
chr4.1 exon 71167172 71167184 71167172 ID=MS.gene76713.t1.exon2;Parent=MS.gene76713.t1
chr4.1 CDS 71167172 71167184 71167172 ID=cds.MS.gene76713.t1;Parent=MS.gene76713.t1
Gene Sequence

>MS.gene76713

ATGGCAAGCATGAAGGTAGCATGTGTTGTTTTGATGATGTGCATGATTGTTGCACCTATGGCAGATGCTGCAATCTCATGTGGACAAGTGAGTGGTGCTCTTTCTCCATGCATTGGATATCTTAGAGGTGGTGCTGGTCCATCTCCAGCATGCTGTGCTGGAGTGAAGAGACTTAACGCCGCCGCCACTACCACACCTGACCGTCAGGCTGCTTGCAACTGCTTGAAAAGTGCTGCTGGTGCTATTTCTGGTTTGAATACAGGCGCAGCTTCTTCTCTTCCTGGCAAATGTGGCGTTAACATTCCTTACAAGATCAGTACCTCCACCAACTGTGCTACGTATGTTTATCTAACTTCAAATTTTCACTTTTTTATCCTCATTTTTCAAGTAGTCTAGTATTATTCATAACATAACAGAAGTAACCAAAGTTAAACAACTAAATTTTTTACCATTTGAGATTGTTGAGGGTAAATCATTCTTTTAATTTTAGGATCACTTTATAGATATGCTATTTTTTACCATTTGCTTAAGGATGTTTTAATACCATATTTTTTTAATAAAATTTTTATATTATTCAATGTTTTTGGGAATTTTTTTGAGATTAAGTCACTACGAATCTACTTTTTTATTGGTCTATAAAATAGAAAATTAATCACTGACTAGGTTTTTTTGTTTTGTTATACTTACAGCATCAGGGCATGA

Protein sequence

>MS.gene76713.t1

MASMKVACVVLMMCMIVAPMADAAISCGQVSGALSPCIGYLRGGAGPSPACCAGVKRLNAAATTTPDRQAACNCLKSAAGAISGLNTGAASSLPGKCGVNIPYKISTSTNCATIRA