AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82253


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82253.t1 MTR_4g029350 93.162 117 8 0 1 117 1 117 1.34e-74 217
MS.gene82253.t1 MTR_4g029390 82.906 117 20 0 1 117 1 117 5.48e-66 195
MS.gene82253.t1 MTR_4g428370 64.103 117 39 2 1 116 58 172 1.97e-44 142
MS.gene82253.t1 MTR_4g027840 61.538 117 42 2 1 116 1 115 1.40e-43 139
MS.gene82253.t1 MTR_4g027800 60.684 117 43 2 1 116 1 115 2.44e-43 138
MS.gene82253.t1 MTR_4g029210 51.456 103 50 0 10 112 9 111 2.02e-31 107
MS.gene82253.t1 MTR_4g028180 45.299 117 55 4 8 116 11 126 1.00e-25 93.6
MS.gene82253.t1 MTR_4g028190 39.831 118 70 1 1 117 5 122 3.01e-24 90.1
MS.gene82253.t1 MTR_7g072930 40.187 107 61 2 10 114 12 117 2.92e-21 83.2
MS.gene82253.t1 MTR_3g467100 34.821 112 73 0 5 116 3 114 9.18e-20 78.2
MS.gene82253.t1 MTR_7g072830 35.043 117 69 3 7 116 4 120 2.81e-19 77.0
MS.gene82253.t1 MTR_7g073150 39.655 116 66 3 2 114 3 117 4.11e-19 77.0
MS.gene82253.t1 MTR_2g062600 35.455 110 68 3 8 115 6 114 4.99e-18 73.9
MS.gene82253.t1 MTR_7g073170 36.937 111 64 5 10 117 12 119 7.47e-18 73.6
MS.gene82253.t1 MTR_3g056640 33.621 116 74 2 3 116 8 122 9.67e-18 73.2
MS.gene82253.t1 MTR_7g072730 33.913 115 69 3 7 114 4 118 3.19e-17 73.2
MS.gene82253.t1 MTR_3g046515 31.579 114 75 2 3 114 8 120 2.21e-16 69.7
MS.gene82253.t1 MTR_3g046350 34.513 113 68 3 4 114 9 117 4.10e-16 68.9
MS.gene82253.t1 MTR_3g046530 31.034 116 77 2 3 116 8 122 5.05e-16 68.9
MS.gene82253.t1 MTR_4g028250 33.051 118 77 2 1 116 1 118 6.76e-16 68.6
MS.gene82253.t1 MTR_3g046540 31.034 116 77 2 3 116 8 122 8.29e-16 68.2
MS.gene82253.t1 MTR_4g028310 35.833 120 71 3 1 116 1 118 1.03e-15 68.6
MS.gene82253.t1 MTR_3g464130 34.951 103 64 2 13 113 5 106 3.31e-15 66.2
MS.gene82253.t1 MTR_4g028360 32.456 114 73 3 4 115 1 112 2.42e-14 64.7
MS.gene82253.t1 MTR_7g451500 33.684 95 62 1 22 115 4 98 2.85e-14 63.5
MS.gene82253.t1 MTR_7g073120 33.898 118 73 3 1 113 1 118 4.47e-14 64.7
MS.gene82253.t1 MTR_3g464110 40.964 83 46 2 34 114 92 173 5.76e-14 64.7
MS.gene82253.t1 MTR_7g072993 35.345 116 71 2 2 113 3 118 1.63e-13 62.8
MS.gene82253.t1 MTR_7g073130 32.456 114 74 3 2 113 3 115 7.23e-13 60.8
MS.gene82253.t1 MTR_7g072900 38.053 113 64 5 4 113 7 116 2.81e-12 58.9
MS.gene82253.t1 MTR_1g040335 38.333 120 67 5 1 115 1 118 3.68e-12 58.9
MS.gene82253.t1 MTR_7g072960 34.783 115 53 4 4 114 7 103 7.27e-12 58.5
MS.gene82253.t1 MTR_7g072980 31.304 115 76 2 2 113 3 117 1.62e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82253.t1 AT5G59320 52.475 101 48 0 16 116 15 115 3.89e-29 102
MS.gene82253.t1 AT5G59310 53.465 101 44 1 16 116 15 112 1.11e-27 98.2
MS.gene82253.t1 AT3G51590 45.872 109 53 3 12 115 8 115 1.17e-24 90.9
MS.gene82253.t1 AT2G38540 45.000 120 60 5 1 116 1 118 2.65e-23 87.4
MS.gene82253.t1 AT4G33355 44.348 115 57 1 1 115 11 118 5.89e-23 86.7
MS.gene82253.t1 AT5G01870 39.655 116 66 3 4 116 2 116 7.81e-22 83.6
MS.gene82253.t1 AT4G33355 43.363 113 57 1 1 113 11 116 9.13e-22 83.6
MS.gene82253.t1 AT3G51600 40.678 118 68 2 1 116 1 118 3.98e-21 81.6
MS.gene82253.t1 AT2G15050 40.179 112 60 4 12 116 11 122 1.36e-20 80.5
MS.gene82253.t1 AT2G38530 43.478 115 58 3 9 116 4 118 1.84e-20 80.1
MS.gene82253.t1 AT2G15050 40.909 110 58 4 12 114 11 120 1.38e-19 78.2
MS.gene82253.t1 AT2G15050 41.748 103 55 3 12 109 11 113 4.27e-19 76.6
MS.gene82253.t1 AT3G08770 43.158 95 50 3 25 116 20 113 7.29e-19 75.9
MS.gene82253.t1 AT3G08770 43.011 93 49 3 25 114 20 111 8.11e-18 73.2
MS.gene82253.t1 AT2G18370 31.897 116 77 2 1 115 1 115 3.60e-15 66.6

Find 33 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTAGAAGTGCTAAACTTAT+AGG 0.238471 4.2:-74808069 None:intergenic
GCACTTCTACCAACTGTAAT+AGG 0.262122 4.2:+74808081 MS.gene82253:CDS
GATAAGTAATGCATGGAATA+AGG 0.317466 4.2:-74807863 None:intergenic
CCATCTCCACAGTGCTGTGC+TGG 0.355566 4.2:+74807903 MS.gene82253:CDS
CATGTGGCTGCCTCTGCTAT+TGG 0.364487 4.2:-74807822 None:intergenic
CCATGCATTACTTATCTTCA+AGG 0.366651 4.2:+74807870 MS.gene82253:CDS
TTGCAAGCAGCCTTGCGAGC+CGG 0.406399 4.2:-74807960 None:intergenic
CAACACTGGCCCGGCTCGCA+AGG 0.417244 4.2:+74807950 MS.gene82253:CDS
CACAGTGCTGTGCTGGAGTA+AGG 0.420837 4.2:+74807910 MS.gene82253:CDS
TGCAAGCAGCCTTGCGAGCC+GGG 0.430405 4.2:-74807959 None:intergenic
GTAGAAGTGCTAAACTTATA+GGG 0.434237 4.2:-74808068 None:intergenic
CTTGCGAGCCGGGCCAGTGT+TGG 0.451576 4.2:-74807949 None:intergenic
CGCCGCCGCCAACACTGGCC+CGG 0.466490 4.2:+74807941 MS.gene82253:CDS
AGCCGGGCCAGTGTTGGCGG+CGG 0.471641 4.2:-74807943 None:intergenic
ACTTATCTTCAAGGAGGTGC+TGG 0.481218 4.2:+74807879 MS.gene82253:CDS
GCGAGCCGGGCCAGTGTTGG+CGG 0.487316 4.2:-74807946 None:intergenic
GCTGCTCTCCCTGGCAAATG+TGG 0.495611 4.2:+74808035 MS.gene82253:CDS
CTTAACGCCGCCGCCAACAC+TGG 0.495662 4.2:+74807936 MS.gene82253:CDS
CCAGCACAGCACTGTGGAGA+TGG 0.501400 4.2:-74807903 None:intergenic
AACCAAGCTGCTGCTCTCCC+TGG 0.514408 4.2:+74808026 MS.gene82253:CDS
TGCCAGGGAGAGCAGCAGCT+TGG 0.525683 4.2:-74808028 None:intergenic
GTTGTTATTCAAACGAGCAA+TGG 0.551011 4.2:-74808006 None:intergenic
AACTGCTTGAAAAGTGCTGC+TGG 0.566956 4.2:+74807981 MS.gene82253:CDS
CCTTGAAGATAAGTAATGCA+TGG 0.589008 4.2:-74807870 None:intergenic
ATCAACTAGTTGACCACATG+TGG 0.592657 4.2:-74807838 None:intergenic
TGTGGTTGCAATTATGTGCA+TGG 0.608819 4.2:+74807785 MS.gene82253:CDS
TAAAACATACCTATTACAGT+TGG 0.611499 4.2:-74808090 None:intergenic
GATTAGTGCACCAATAGCAG+AGG 0.666548 4.2:+74807812 MS.gene82253:CDS
TGCATTACTTATCTTCAAGG+AGG 0.675764 4.2:+74807873 MS.gene82253:CDS
AAGCTTGAGAGTTACAATTG+TGG 0.688534 4.2:+74807767 MS.gene82253:CDS
ATAGCAGAGGCAGCCACATG+TGG 0.707329 4.2:+74807825 MS.gene82253:CDS
CTTACTCCAGCACAGCACTG+TGG 0.718135 4.2:-74807909 None:intergenic
GGTTGCAATTATGTGCATGG+TGG 0.720687 4.2:+74807788 MS.gene82253:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAGTTATATATATAATTA+GGG - chr4.2:74808125-74808144 None:intergenic 10.0%
!! TAAGAGTTATATATATAATT+AGG - chr4.2:74808126-74808145 None:intergenic 10.0%
!! AACAATACCTTAAAAAAAAA+AGG + chr4.2:74808181-74808200 MS.gene82253:intron 15.0%
!! AATTAGGTATTATTATAACA+GGG + chr4.2:74808426-74808445 MS.gene82253:intron 15.0%
!! ATTGAAATTGAAATGATAAT+TGG + chr4.2:74808344-74808363 MS.gene82253:intron 15.0%
!! TAATTAGGTATTATTATAAC+AGG + chr4.2:74808425-74808444 MS.gene82253:intron 15.0%
!! TATAACTCTTAAAAATTTCT+AGG + chr4.2:74808135-74808154 MS.gene82253:intron 15.0%
!!! ATATGTGTCATTTATTTTTT+TGG + chr4.2:74808377-74808396 MS.gene82253:intron 15.0%
!!! CATTTTACCTTTTTTTTTTA+AGG - chr4.2:74808191-74808210 None:intergenic 15.0%
!! AGCAGAAAATCAATTTATAA+TGG + chr4.2:74808233-74808252 MS.gene82253:intron 20.0%
!! TATTGTTTATATTTGTTTCC+AGG - chr4.2:74808168-74808187 None:intergenic 20.0%
!!! TAGTATTACTTTCATTTTGA+TGG - chr4.2:74808549-74808568 None:intergenic 20.0%
!!! TTGAAGCTTTATAAAATAAG+TGG + chr4.2:74808487-74808506 MS.gene82253:intron 20.0%
! GAAATTGAAATGATAATTGG+AGG + chr4.2:74808347-74808366 MS.gene82253:intron 25.0%
! TAAAACATACCTATTACAGT+TGG - chr4.2:74808093-74808112 None:intergenic 25.0%
!! TTTTTGTCATCATCTTAGTT+TGG - chr4.2:74808313-74808332 None:intergenic 25.0%
!!! AGGGTTTTGTTTTAACTAAA+AGG + chr4.2:74808445-74808464 MS.gene82253:intron 25.0%
!!! ATGATTGACTTATGCAATTT+TGG + chr4.2:74808519-74808538 MS.gene82253:intron 25.0%
!!! GTACATGTGTCATTTTAATT+AGG + chr4.2:74808410-74808429 MS.gene82253:intron 25.0%
AAAGAAACATCTATATGCTC+AGG - chr4.2:74808685-74808704 None:intergenic 30.0%
ATTGAAATGATAATTGGAGG+AGG + chr4.2:74808350-74808369 MS.gene82253:intron 30.0%
GATAAGTAATGCATGGAATA+AGG - chr4.2:74807866-74807885 None:intergenic 30.0%
!! AAATTTCTAGGATTCAAACC+TGG + chr4.2:74808147-74808166 MS.gene82253:intron 30.0%
!! GTAGAAGTGCTAAACTTATA+GGG - chr4.2:74808071-74808090 None:intergenic 30.0%
AAACATCTATATGCTCAGGA+AGG - chr4.2:74808681-74808700 None:intergenic 35.0%
AACATCTATATGCTCAGGAA+GGG - chr4.2:74808680-74808699 None:intergenic 35.0%
AAGCTTGAGAGTTACAATTG+TGG + chr4.2:74807767-74807786 MS.gene82253:CDS 35.0%
CCATGCATTACTTATCTTCA+AGG + chr4.2:74807870-74807889 MS.gene82253:CDS 35.0%
CCTTGAAGATAAGTAATGCA+TGG - chr4.2:74807873-74807892 None:intergenic 35.0%
TGCATTACTTATCTTCAAGG+AGG + chr4.2:74807873-74807892 MS.gene82253:CDS 35.0%
! GTTGTTATTCAAACGAGCAA+TGG - chr4.2:74808009-74808028 None:intergenic 35.0%
! TTGTCATCATCTTAGTTTGG+AGG - chr4.2:74808310-74808329 None:intergenic 35.0%
! TTTTAACACCACATTTGCCA+GGG - chr4.2:74808046-74808065 None:intergenic 35.0%
!! ATTTTAACACCACATTTGCC+AGG - chr4.2:74808047-74808066 None:intergenic 35.0%
!! GGTAGAAGTGCTAAACTTAT+AGG - chr4.2:74808072-74808091 None:intergenic 35.0%
ATCAACTAGTTGACCACATG+TGG - chr4.2:74807841-74807860 None:intergenic 40.0%
GCACTTCTACCAACTGTAAT+AGG + chr4.2:74808081-74808100 MS.gene82253:CDS 40.0%
TGTGGTTGCAATTATGTGCA+TGG + chr4.2:74807785-74807804 MS.gene82253:CDS 40.0%
ACTTGATGCTGCAATACAGC+AGG - chr4.2:74808766-74808785 None:intergenic 45.0%
GATTAGTGCACCAATAGCAG+AGG + chr4.2:74807812-74807831 MS.gene82253:CDS 45.0%
GGTTGCAATTATGTGCATGG+TGG + chr4.2:74807788-74807807 MS.gene82253:CDS 45.0%
! ACTTATCTTCAAGGAGGTGC+TGG + chr4.2:74807879-74807898 MS.gene82253:CDS 45.0%
!! AACTGCTTGAAAAGTGCTGC+TGG + chr4.2:74807981-74808000 MS.gene82253:CDS 45.0%
ATAGCAGAGGCAGCCACATG+TGG + chr4.2:74807825-74807844 MS.gene82253:CDS 55.0%
CACAGTGCTGTGCTGGAGTA+AGG + chr4.2:74807910-74807929 MS.gene82253:CDS 55.0%
CATGTGGCTGCCTCTGCTAT+TGG - chr4.2:74807825-74807844 None:intergenic 55.0%
CTTACTCCAGCACAGCACTG+TGG - chr4.2:74807912-74807931 None:intergenic 55.0%
AACCAAGCTGCTGCTCTCCC+TGG + chr4.2:74808026-74808045 MS.gene82253:CDS 60.0%
CCAGCACAGCACTGTGGAGA+TGG - chr4.2:74807906-74807925 None:intergenic 60.0%
CCATCTCCACAGTGCTGTGC+TGG + chr4.2:74807903-74807922 MS.gene82253:CDS 60.0%
GCTGCTCTCCCTGGCAAATG+TGG + chr4.2:74808035-74808054 MS.gene82253:CDS 60.0%
TTGCAAGCAGCCTTGCGAGC+CGG - chr4.2:74807963-74807982 None:intergenic 60.0%
CTTAACGCCGCCGCCAACAC+TGG + chr4.2:74807936-74807955 MS.gene82253:CDS 65.0%
TGCAAGCAGCCTTGCGAGCC+GGG - chr4.2:74807962-74807981 None:intergenic 65.0%
TGCCAGGGAGAGCAGCAGCT+TGG - chr4.2:74808031-74808050 None:intergenic 65.0%
CTTGCGAGCCGGGCCAGTGT+TGG - chr4.2:74807952-74807971 None:intergenic 70.0%
! CAACACTGGCCCGGCTCGCA+AGG + chr4.2:74807950-74807969 MS.gene82253:CDS 70.0%
! GCGAGCCGGGCCAGTGTTGG+CGG - chr4.2:74807949-74807968 None:intergenic 75.0%
!! AGCCGGGCCAGTGTTGGCGG+CGG - chr4.2:74807946-74807965 None:intergenic 75.0%
! CGCCGCCGCCAACACTGGCC+CGG + chr4.2:74807941-74807960 MS.gene82253:CDS 80.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 74807762 74808790 74807762 ID=MS.gene82253
chr4.2 mRNA 74807762 74808790 74807762 ID=MS.gene82253.t1;Parent=MS.gene82253
chr4.2 exon 74807762 74808102 74807762 ID=MS.gene82253.t1.exon1;Parent=MS.gene82253.t1
chr4.2 CDS 74807762 74808102 74807762 ID=cds.MS.gene82253.t1;Parent=MS.gene82253.t1
chr4.2 exon 74808778 74808790 74808778 ID=MS.gene82253.t1.exon2;Parent=MS.gene82253.t1
chr4.2 CDS 74808778 74808790 74808778 ID=cds.MS.gene82253.t1;Parent=MS.gene82253.t1
Gene Sequence

>MS.gene82253

ATGGCAAGCTTGAGAGTTACAATTGTGGTTGCAATTATGTGCATGGTGGTGATTAGTGCACCAATAGCAGAGGCAGCCACATGTGGTCAACTAGTTGATAGCCTTATTCCATGCATTACTTATCTTCAAGGAGGTGCTGGTCCATCTCCACAGTGCTGTGCTGGAGTAAGGAAACTTAACGCCGCCGCCAACACTGGCCCGGCTCGCAAGGCTGCTTGCAACTGCTTGAAAAGTGCTGCTGGTACCATTGCTCGTTTGAATAACAACCAAGCTGCTGCTCTCCCTGGCAAATGTGGTGTTAAAATTCCCTATAAGTTTAGCACTTCTACCAACTGTAATAGGTATGTTTTATATCTCACTCCCTAATTATATATATAACTCTTAAAAATTTCTAGGATTCAAACCTGGAAACAAATATAAACAATACCTTAAAAAAAAAAGGTAAAATGAAATGAAACAAAATGATGTTGAAGCAGAAAATCAATTTATAATGGAATGAGAAAAAAACTTTTATTTCATATCATATTCTGAAACTGAACATGTGTCCTCCAAACTAAGATGATGACAAAAATTAAGTGAGACATTGAAATTGAAATGATAATTGGAGGAGGTCAAATATGTGTCATTTATTTTTTTGGTATAATATATGTACATGTGTCATTTTAATTAGGTATTATTATAACAGGGTTTTGTTTTAACTAAAAGGTATGAAAAAAAAAAAAGAGTTGAAGCTTTATAAAATAAGTGGAAACAAATTATGATTGACTTATGCAATTTTGGACAGCCATCAAAATGAAAGTAATACTATAAAATTAATAAATAAAATTATAAAATAATCTCAATTTTCATTTGACATGTGCTTTATTTTTTTTTTTTGAAGAAACTTGTGCTTTATTTTTTATTCATATATATTAGCCCTTCCTGAGCATATAGATGTTTCTTTTATTTTTTTTCTCTCATATTTTAAATTAAAAATCATATATAACTTAATTTGCTTGTTTCCTGCTGTATTGCAGCATCAAGTTCTAA

Protein sequence

>MS.gene82253.t1

MASLRVTIVVAIMCMVVISAPIAEAATCGQLVDSLIPCITYLQGGAGPSPQCCAGVRKLNAAANTGPARKAACNCLKSAAGTIARLNNNQAAALPGKCGVKIPYKFSTSTNCNSIKF