AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene76705


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76705.t1 MTR_4g028180 95.238 126 5 1 1 125 1 126 8.20e-83 238
MS.gene76705.t1 MTR_7g073170 46.281 121 59 2 4 124 3 117 4.29e-33 112
MS.gene76705.t1 MTR_7g073150 45.000 120 61 2 4 123 3 117 1.22e-30 106
MS.gene76705.t1 MTR_4g428370 46.552 116 56 3 9 124 62 171 7.19e-29 103
MS.gene76705.t1 MTR_7g072930 43.089 123 64 3 4 125 3 120 8.02e-27 97.4
MS.gene76705.t1 MTR_4g028190 39.024 123 71 1 1 123 1 119 1.61e-26 95.9
MS.gene76705.t1 MTR_4g027800 41.880 117 62 3 9 125 5 115 3.00e-26 95.1
MS.gene76705.t1 MTR_4g027840 42.735 117 61 3 9 125 5 115 9.07e-26 94.7
MS.gene76705.t1 MTR_4g029350 41.803 122 64 3 4 125 2 116 2.01e-24 90.5
MS.gene76705.t1 MTR_2g062600 38.333 120 67 3 6 124 1 114 1.64e-23 88.2
MS.gene76705.t1 MTR_1g040335 44.715 123 61 3 4 125 3 119 2.09e-23 87.8
MS.gene76705.t1 MTR_7g072900 42.017 119 64 2 4 122 3 116 3.67e-23 87.4
MS.gene76705.t1 MTR_7g072830 38.211 123 70 3 4 125 3 120 6.02e-23 86.7
MS.gene76705.t1 MTR_4g029390 41.803 122 64 3 4 125 2 116 6.26e-23 86.7
MS.gene76705.t1 MTR_7g072993 43.443 122 62 3 4 123 3 119 1.47e-22 86.3
MS.gene76705.t1 MTR_7g073130 41.600 125 62 4 4 125 3 119 1.28e-21 83.6
MS.gene76705.t1 MTR_4g029210 38.938 113 63 3 9 121 5 111 1.38e-21 83.2
MS.gene76705.t1 MTR_7g072730 36.364 121 71 3 4 123 3 118 2.41e-21 84.0
MS.gene76705.t1 MTR_7g072960 39.516 124 53 3 6 125 1 106 7.21e-20 79.3
MS.gene76705.t1 MTR_7g073060 36.066 122 73 2 4 125 3 119 7.38e-20 79.0
MS.gene76705.t1 MTR_7g072980 36.667 120 70 3 4 122 3 117 2.54e-19 78.2
MS.gene76705.t1 MTR_7g073120 37.600 125 70 4 1 123 1 119 2.93e-19 78.2
MS.gene76705.t1 MTR_7g073100 36.667 120 71 2 4 123 3 117 6.16e-19 77.4
MS.gene76705.t1 MTR_7g072810 39.669 121 61 4 4 123 3 112 1.76e-18 75.5
MS.gene76705.t1 MTR_7g072760 37.097 124 72 3 4 125 3 122 7.24e-17 71.2
MS.gene76705.t1 MTR_7g073030 35.833 120 72 2 4 123 3 117 3.31e-16 70.5
MS.gene76705.t1 MTR_7g451500 29.787 94 62 1 31 124 9 98 4.13e-15 66.2
MS.gene76705.t1 MTR_3g467100 32.000 125 72 5 1 124 1 113 3.13e-14 64.3
MS.gene76705.t1 MTR_1g040330 38.211 123 69 3 4 125 3 119 7.30e-14 63.5
MS.gene76705.t1 MTR_3g056640 31.624 117 74 2 10 125 11 122 3.74e-13 61.6
MS.gene76705.t1 MTR_3g046515 28.455 123 82 2 2 123 3 120 3.78e-13 61.6
MS.gene76705.t1 MTR_3g046530 28.000 125 84 2 2 125 3 122 6.89e-13 60.8
MS.gene76705.t1 MTR_3g046540 28.000 125 84 2 2 125 3 122 8.10e-13 60.8
MS.gene76705.t1 MTR_4g028250 32.773 119 73 4 9 125 5 118 6.07e-11 55.8
MS.gene76705.t1 MTR_3g464130 33.333 93 56 2 31 122 19 106 7.09e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene76705.t1 AT2G15050 50.833 120 53 3 9 125 6 122 8.10e-33 112
MS.gene76705.t1 AT2G15050 50.847 118 52 3 9 123 6 120 7.32e-32 109
MS.gene76705.t1 AT2G15050 51.351 111 48 3 9 116 6 113 5.75e-30 104
MS.gene76705.t1 AT2G38540 43.590 117 62 2 9 125 6 118 1.34e-29 103
MS.gene76705.t1 AT5G01870 44.348 115 58 4 11 125 8 116 1.12e-28 101
MS.gene76705.t1 AT3G08770 48.515 101 48 3 25 125 17 113 3.27e-27 97.4
MS.gene76705.t1 AT3G08770 49.495 99 46 3 25 123 17 111 1.21e-26 96.3
MS.gene76705.t1 AT5G59320 46.847 111 53 3 15 125 11 115 6.14e-26 94.4
MS.gene76705.t1 AT5G59310 46.364 110 47 4 16 125 15 112 4.62e-25 92.0
MS.gene76705.t1 AT2G38530 42.735 117 63 2 9 125 6 118 5.47e-24 89.4
MS.gene76705.t1 AT3G51600 42.735 117 63 2 9 125 6 118 6.66e-23 86.7
MS.gene76705.t1 AT3G51590 39.316 117 65 3 9 125 6 116 1.56e-22 85.9
MS.gene76705.t1 AT2G18370 35.897 117 68 2 10 124 4 115 1.87e-20 80.5
MS.gene76705.t1 AT4G33355 35.246 122 72 3 4 125 5 119 1.23e-16 70.5
MS.gene76705.t1 AT4G33355 35.294 119 70 3 4 122 5 116 1.42e-15 67.8

Find 32 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTTTGAGCCATAGTATTA+AGG 0.282339 4.1:-71291046 None:intergenic
ATTGTGTTTCTTGTAACTTT+TGG 0.294759 4.1:+71290900 MS.gene76705:CDS
GGCAACTAGGCTTGTTTGTT+TGG 0.295657 4.1:+71290875 MS.gene76705:CDS
TACCTGTTACAGTCTGTATT+AGG 0.301828 4.1:-71291206 None:intergenic
ACCTGTTACAGTCTGTATTA+GGG 0.370690 4.1:-71291205 None:intergenic
GCCCTAATACAGACTGTAAC+AGG 0.371378 4.1:+71291204 MS.gene76705:CDS
GAAGACCAGCAGCAAGGTTA+AGG 0.386841 4.1:-71291145 None:intergenic
CATCATGGGTTACTCAACTA+TGG 0.397979 4.1:+71290854 None:intergenic
GTAACAGCTGCTTCAGCCTT+AGG 0.410392 4.1:-71290924 None:intergenic
AGTAAGGAACCTTAATACTA+TGG 0.425103 4.1:+71291037 MS.gene76705:CDS
AACAGCTCTACGATCATTTG+TGG 0.431202 4.1:-71291066 None:intergenic
AGTCTGTATTAGGGCTGATC+TGG 0.446804 4.1:-71291196 None:intergenic
TGTGTTTCTTACATTATGAC+TGG 0.454062 4.1:+71290981 MS.gene76705:CDS
CACAGTGTTGTAATGGAGTA+AGG 0.461553 4.1:+71291021 MS.gene76705:CDS
TTAAACCTTAACCTTGCTGC+TGG 0.479414 4.1:+71291140 MS.gene76705:CDS
GTTAACAGCATTCTTAATGC+AGG 0.496302 4.1:-71291093 None:intergenic
AAGAATGCTGTTAACCAAAG+TGG 0.504107 4.1:+71291101 MS.gene76705:CDS
GTATTAGGGCTGATCTGGTA+AGG 0.522276 4.1:-71291191 None:intergenic
ATGTTAACACCACATTTCCT+TGG 0.522311 4.1:-71291167 None:intergenic
GTTCCTGCACAGTGTTGTAA+TGG 0.544737 4.1:+71291014 MS.gene76705:CDS
ACCTTGCTGCTGGTCTTCCA+AGG 0.551966 4.1:+71291150 MS.gene76705:CDS
TAGTGTAACTGAAACCACTT+TGG 0.564886 4.1:-71291115 None:intergenic
GTTACTCAACTATGGCAACT+AGG 0.565651 4.1:+71290862 MS.gene76705:CDS
GTTTCTTACATTATGACTGG+TGG 0.583866 4.1:+71290984 MS.gene76705:CDS
GCTGGTCTTCCAAGGAAATG+TGG 0.584199 4.1:+71291158 MS.gene76705:CDS
TTTCTTACATTATGACTGGT+GGG 0.587974 4.1:+71290985 MS.gene76705:CDS
ACTCCATTACAACACTGTGC+AGG 0.590449 4.1:-71291017 None:intergenic
GCTTGTCACAACTTGACCAC+AGG 0.613475 4.1:-71290949 None:intergenic
TCCTTGGAAGACCAGCAGCA+AGG 0.622205 4.1:-71291151 None:intergenic
GAAGCAGCTGTTACATCCTG+TGG 0.634952 4.1:+71290933 MS.gene76705:CDS
GTCATAATGTAAGAAACACA+AGG 0.653065 4.1:-71290978 None:intergenic
TCATAATGTAAGAAACACAA+GGG 0.708823 4.1:-71290977 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAATAAAGTTTGTTACA+TGG - chr4.1:71291553-71291572 None:intergenic 15.0%
!!! GTAACAAACTTTATTTTTTA+TGG + chr4.1:71291554-71291573 MS.gene76705:intron 15.0%
!! AGAAAATACATATATACTTG+AGG - chr4.1:71291283-71291302 None:intergenic 20.0%
!! GAAAATACATATATACTTGA+GGG - chr4.1:71291282-71291301 None:intergenic 20.0%
! TCATAATGTAAGAAACACAA+GGG - chr4.1:71290980-71290999 None:intergenic 25.0%
!! ATTGTGTTTCTTGTAACTTT+TGG + chr4.1:71290900-71290919 MS.gene76705:CDS 25.0%
AGTAAGGAACCTTAATACTA+TGG + chr4.1:71291037-71291056 MS.gene76705:CDS 30.0%
GTCATAATGTAAGAAACACA+AGG - chr4.1:71290981-71291000 None:intergenic 30.0%
TAATCTTGTCACACTTAGTA+TGG - chr4.1:71291484-71291503 None:intergenic 30.0%
TATCATGTATGATCGAGATT+GGG + chr4.1:71291393-71291412 MS.gene76705:intron 30.0%
TGTGTTTCTTACATTATGAC+TGG + chr4.1:71290981-71291000 MS.gene76705:CDS 30.0%
TTTCTTACATTATGACTGGT+GGG + chr4.1:71290985-71291004 MS.gene76705:CDS 30.0%
AAGAATGCTGTTAACCAAAG+TGG + chr4.1:71291101-71291120 MS.gene76705:CDS 35.0%
AAGACTCGTCAGACATATAT+AGG - chr4.1:71291589-71291608 None:intergenic 35.0%
ACCTGTTACAGTCTGTATTA+GGG - chr4.1:71291208-71291227 None:intergenic 35.0%
AGTGTGACAAGATTAAAGTG+TGG + chr4.1:71291489-71291508 MS.gene76705:intron 35.0%
ATGTTAACACCACATTTCCT+TGG - chr4.1:71291170-71291189 None:intergenic 35.0%
GTATCATGTATGATCGAGAT+TGG + chr4.1:71291392-71291411 MS.gene76705:intron 35.0%
GTGTGACAAGATTAAAGTGT+GGG + chr4.1:71291490-71291509 MS.gene76705:intron 35.0%
GTTAACAGCATTCTTAATGC+AGG - chr4.1:71291096-71291115 None:intergenic 35.0%
GTTTCTTACATTATGACTGG+TGG + chr4.1:71290984-71291003 MS.gene76705:CDS 35.0%
TACCTGTTACAGTCTGTATT+AGG - chr4.1:71291209-71291228 None:intergenic 35.0%
TAGTGTAACTGAAACCACTT+TGG - chr4.1:71291118-71291137 None:intergenic 35.0%
TGGTTTGAGCCATAGTATTA+AGG - chr4.1:71291049-71291068 None:intergenic 35.0%
! TCTTGTAACTTTTGGTCCTA+AGG + chr4.1:71290908-71290927 MS.gene76705:CDS 35.0%
AACAGCTCTACGATCATTTG+TGG - chr4.1:71291069-71291088 None:intergenic 40.0%
GATACGATCGAGAATGAGTT+GGG - chr4.1:71291377-71291396 None:intergenic 40.0%
GTTACTCAACTATGGCAACT+AGG + chr4.1:71290862-71290881 MS.gene76705:CDS 40.0%
TGATACGATCGAGAATGAGT+TGG - chr4.1:71291378-71291397 None:intergenic 40.0%
TTAAACCTTAACCTTGCTGC+TGG + chr4.1:71291140-71291159 MS.gene76705:CDS 40.0%
! CACAGTGTTGTAATGGAGTA+AGG + chr4.1:71291021-71291040 MS.gene76705:CDS 40.0%
ACTCCATTACAACACTGTGC+AGG - chr4.1:71291020-71291039 None:intergenic 45.0%
AGTCTGTATTAGGGCTGATC+TGG - chr4.1:71291199-71291218 None:intergenic 45.0%
GCCCTAATACAGACTGTAAC+AGG + chr4.1:71291204-71291223 MS.gene76705:CDS 45.0%
GTATTAGGGCTGATCTGGTA+AGG - chr4.1:71291194-71291213 None:intergenic 45.0%
GTTCCTGCACAGTGTTGTAA+TGG + chr4.1:71291014-71291033 MS.gene76705:CDS 45.0%
TCATATGTAGCCCTGCAGAT+CGG + chr4.1:71291351-71291370 MS.gene76705:intron 45.0%
! GGCAACTAGGCTTGTTTGTT+TGG + chr4.1:71290875-71290894 MS.gene76705:CDS 45.0%
GAAGACCAGCAGCAAGGTTA+AGG - chr4.1:71291148-71291167 None:intergenic 50.0%
GAAGCAGCTGTTACATCCTG+TGG + chr4.1:71290933-71290952 MS.gene76705:CDS 50.0%
GCTTGTCACAACTTGACCAC+AGG - chr4.1:71290952-71290971 None:intergenic 50.0%
GTAACAGCTGCTTCAGCCTT+AGG - chr4.1:71290927-71290946 None:intergenic 50.0%
!! GCTGGTCTTCCAAGGAAATG+TGG + chr4.1:71291158-71291177 MS.gene76705:CDS 50.0%
TCCTTGGAAGACCAGCAGCA+AGG - chr4.1:71291154-71291173 None:intergenic 55.0%
! AATGAGTTGGGCCGATCTGC+AGG - chr4.1:71291365-71291384 None:intergenic 55.0%
! ATGAGTTGGGCCGATCTGCA+GGG - chr4.1:71291364-71291383 None:intergenic 55.0%
!! ACCTTGCTGCTGGTCTTCCA+AGG + chr4.1:71291150-71291169 MS.gene76705:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 71290858 71291638 71290858 ID=MS.gene76705
chr4.1 mRNA 71290858 71291638 71290858 ID=MS.gene76705.t1;Parent=MS.gene76705
chr4.1 exon 71290858 71291225 71290858 ID=MS.gene76705.t1.exon1;Parent=MS.gene76705.t1
chr4.1 CDS 71290858 71291225 71290858 ID=cds.MS.gene76705.t1;Parent=MS.gene76705.t1
chr4.1 exon 71291629 71291638 71291629 ID=MS.gene76705.t1.exon2;Parent=MS.gene76705.t1
chr4.1 CDS 71291629 71291638 71291629 ID=cds.MS.gene76705.t1;Parent=MS.gene76705.t1
Gene Sequence

>MS.gene76705

ATGGGTTACTCAACTATGGCAACTAGGCTTGTTTGTTTGGCAATTGTGTTTCTTGTAACTTTTGGTCCTAAGGCTGAAGCAGCTGTTACATCCTGTGGTCAAGTTGTGACAAGCTTATACCCTTGTGTTTCTTACATTATGACTGGTGGGAACACTGTTCCTGCACAGTGTTGTAATGGAGTAAGGAACCTTAATACTATGGCTCAAACCACAAATGATCGTAGAGCTGTTTGTACCTGCATTAAGAATGCTGTTAACCAAAGTGGTTTCAGTTACACTAACTTAAACCTTAACCTTGCTGCTGGTCTTCCAAGGAAATGTGGTGTTAACATTCCTTACCAGATCAGCCCTAATACAGACTGTAACAGGTACTACTTATAATATTTACATATCTATATACACATAACACATGTAATGTAAACCCTCAAGTATATATGTATTTTCTCACAAAAATAATTACTTTTTTAATTTTCTCTAAAAAATGCTACTTAAATCATATGTAGCCCTGCAGATCGGCCCAACTCATTCTCGATCGTATCATGTATGATCGAGATTGGGCTAGATCGAGATGTAGAGCTGTAGATAAGTCATTTTACTTTAATGATATGTTATGATATAAGATACCATACTAAGTGTGACAAGATTAAAGTGTGGGAGTACTACAAGTTAAAAATGAGTTACTGATTTTTTTTCCATGTAACAAACTTTATTTTTTATGGAACATGTTTCCTATATATGTCTGACGAGTCTTTTTTTGTTGTTTTGATGCAGTGTGCACTGA

Protein sequence

>MS.gene76705.t1

MGYSTMATRLVCLAIVFLVTFGPKAEAAVTSCGQVVTSLYPCVSYIMTGGNTVPAQCCNGVRNLNTMAQTTNDRRAVCTCIKNAVNQSGFSYTNLNLNLAAGLPRKCGVNIPYQISPNTDCNSVH