AlfalfaGEDB | MS.gene82219

M. sativa cultivar XinJiangDaYe / MS.gene82219

KEGG: -
GO: GO:0003674 GO:0005488 GO:0006810 GO:0006869 GO:0008150 GO:0008289 GO:0051179 GO:0051234 GO:0071702
PFAM: PF00234.25

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82219.t1	KEH29195.1	87.9	116	14	0	1	116	58	173	6.50E-48	199.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82219.t1	A0AT31	77.0	113	26	0	3	115	4	116	2.0e-42	172.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82219.t1	A0A072UTH7	87.9	116	14	0	1	116	58	173	4.7e-48	199.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050128	MS.gene82219	0.804342	2.26E-49	-1.69E-46
MS.gene050301	MS.gene82219	0.828252	9.97E-55	-1.69E-46
MS.gene050397	MS.gene82219	0.807648	4.56E-50	-1.69E-46
MS.gene050404	MS.gene82219	0.800076	1.71E-48	-1.69E-46
MS.gene052178	MS.gene82219	0.837828	4.13E-57	-1.69E-46
MS.gene052310	MS.gene82219	0.803474	3.43E-49	-1.69E-46
MS.gene052340	MS.gene82219	0.842233	2.93E-58	-1.69E-46
MS.gene053209	MS.gene82219	0.897005	2.14E-76	-1.69E-46
MS.gene053557	MS.gene82219	0.843814	1.11E-58	-1.69E-46
MS.gene053928	MS.gene82219	0.801187	1.01E-48	-1.69E-46
MS.gene055116	MS.gene82219	0.812131	4.95E-51	-1.69E-46
MS.gene055277	MS.gene82219	0.83003	3.69E-55	-1.69E-46
MS.gene055604	MS.gene82219	0.803363	3.61E-49	-1.69E-46
MS.gene056191	MS.gene82219	0.818957	1.49E-52	-1.69E-46
MS.gene056410	MS.gene82219	0.824387	8.30E-54	-1.69E-46
MS.gene056796	MS.gene82219	0.802366	5.80E-49	-1.69E-46
MS.gene05702	MS.gene82219	0.810437	1.15E-50	-1.69E-46
MS.gene057796	MS.gene82219	0.812962	3.26E-51	-1.69E-46
MS.gene058082	MS.gene82219	0.809471	1.86E-50	-1.69E-46
MS.gene05811	MS.gene82219	0.808637	2.81E-50	-1.69E-46
MS.gene059518	MS.gene82219	0.806778	6.97E-50	-1.69E-46
MS.gene059688	MS.gene82219	0.810403	1.17E-50	-1.69E-46
MS.gene059692	MS.gene82219	0.838472	2.82E-57	-1.69E-46
MS.gene059695	MS.gene82219	0.833846	4.22E-56	-1.69E-46
MS.gene059894	MS.gene82219	0.83831	3.10E-57	-1.69E-46
MS.gene059974	MS.gene82219	0.891326	4.40E-74	-1.69E-46
MS.gene06037	MS.gene82219	0.823527	1.32E-53	-1.69E-46
MS.gene061049	MS.gene82219	0.829665	4.53E-55	-1.69E-46
MS.gene061154	MS.gene82219	0.818042	2.41E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene82256	MS.gene82219	PPI
MS.gene48835	MS.gene82219	PPI
MS.gene82221	MS.gene82219	PPI
MS.gene82219	MS.gene53309	PPI
MS.gene53311	MS.gene82219	PPI
MS.gene48796	MS.gene82219	PPI
MS.gene76713	MS.gene82219	PPI
MS.gene82219	MS.gene76714	PPI
MS.gene76714	MS.gene82219	PPI
MS.gene82222	MS.gene82219	PPI
MS.gene48829	MS.gene82219	PPI
MS.gene76745	MS.gene82219	PPI
MS.gene82253	MS.gene82219	PPI
MS.gene77022	MS.gene82219	PPI
MS.gene77025	MS.gene82219	PPI
MS.gene48828	MS.gene82219	PPI
MS.gene76705	MS.gene82219	PPI
MS.gene48797	MS.gene82219	PPI
MS.gene53312	MS.gene82219	PPI
MS.gene76710	MS.gene82219	PPI
MS.gene53309	MS.gene82219	PPI
MS.gene76747	MS.gene82219	PPI
MS.gene48831	MS.gene82219	PPI
MS.gene53305	MS.gene82219	PPI
MS.gene82219	MS.gene82222	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82219.t1	MTR_4g428370	87.931	116	14	0	1	116	58	173	1.25e-69	206
MS.gene82219.t1	MTR_4g027840	86.957	115	15	0	1	115	1	115	1.35e-66	197
MS.gene82219.t1	MTR_4g027800	86.207	116	16	0	1	116	1	116	1.72e-66	196
MS.gene82219.t1	MTR_4g029390	66.667	117	36	2	1	115	1	116	1.03e-47	149
MS.gene82219.t1	MTR_4g029350	66.667	117	36	2	1	115	1	116	1.24e-46	146
MS.gene82219.t1	MTR_4g028190	48.246	114	57	2	1	112	5	118	5.72e-31	107
MS.gene82219.t1	MTR_4g029210	48.214	112	58	0	1	112	1	112	2.13e-30	105
MS.gene82219.t1	MTR_7g072930	47.321	112	56	2	4	113	7	117	1.07e-28	102
MS.gene82219.t1	MTR_4g028180	43.220	118	60	3	5	115	9	126	2.91e-27	97.4
MS.gene82219.t1	MTR_7g072830	40.351	114	66	1	4	115	7	120	4.63e-27	97.1
MS.gene82219.t1	MTR_7g073150	45.690	116	58	4	2	113	3	117	4.94e-27	97.1
MS.gene82219.t1	MTR_7g072730	41.071	112	64	2	4	113	7	118	6.40e-26	95.5
MS.gene82219.t1	MTR_4g028310	40.336	119	68	1	1	116	1	119	5.60e-25	92.0
MS.gene82219.t1	MTR_7g073170	41.593	113	62	3	4	114	7	117	5.81e-25	91.7
MS.gene82219.t1	MTR_2g062600	41.739	115	65	2	1	114	1	114	1.17e-24	90.5
MS.gene82219.t1	MTR_7g073120	37.168	113	69	1	1	111	5	117	3.61e-21	82.8
MS.gene82219.t1	MTR_4g028250	39.831	118	68	2	1	115	1	118	6.59e-21	81.3
MS.gene82219.t1	MTR_7g072900	43.750	112	58	4	4	112	7	116	1.22e-20	80.5
MS.gene82219.t1	MTR_7g072993	42.857	112	59	3	4	111	7	117	8.94e-20	79.0
MS.gene82219.t1	MTR_3g467100	36.607	112	70	1	5	115	3	114	4.05e-19	76.6
MS.gene82219.t1	MTR_4g028360	36.283	113	69	3	4	114	1	112	1.23e-17	73.2
MS.gene82219.t1	MTR_7g072960	39.252	107	49	3	9	113	11	103	1.90e-17	72.8
MS.gene82219.t1	MTR_7g451500	38.043	92	56	1	24	114	7	98	3.34e-17	71.2
MS.gene82219.t1	MTR_7g072980	37.500	112	66	3	4	112	7	117	8.24e-17	71.2
MS.gene82219.t1	MTR_7g073130	38.739	111	64	4	4	112	7	115	1.08e-16	70.5
MS.gene82219.t1	MTR_1g040335	41.071	112	63	2	5	114	8	118	2.76e-16	69.3
MS.gene82219.t1	MTR_7g073060	38.053	113	67	3	4	114	7	118	7.07e-16	68.2
MS.gene82219.t1	MTR_7g073100	37.168	113	64	2	4	112	7	116	7.86e-16	68.9
MS.gene82219.t1	MTR_7g072810	39.091	110	59	3	4	111	7	110	5.80e-15	66.2
MS.gene82219.t1	MTR_3g056640	34.821	112	67	3	9	115	12	122	1.57e-14	65.1
MS.gene82219.t1	MTR_3g046515	34.821	112	67	4	3	110	10	119	9.26e-14	62.8
MS.gene82219.t1	MTR_3g046530	30.769	117	75	3	4	115	7	122	2.36e-13	62.0
MS.gene82219.t1	MTR_7g073030	35.455	110	68	3	4	111	7	115	2.96e-13	62.4
MS.gene82219.t1	MTR_3g464130	37.778	90	51	3	25	110	18	106	6.57e-13	60.5
MS.gene82219.t1	MTR_3g046350	31.373	102	64	3	10	106	13	113	1.66e-12	59.7
MS.gene82219.t1	MTR_3g046540	33.043	115	67	5	4	110	7	119	1.94e-12	59.7
MS.gene82219.t1	MTR_3g464110	40.244	82	44	3	33	110	92	172	2.54e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82219.t1	AT5G59320	60.185	108	41	1	8	115	10	115	2.33e-39	127
MS.gene82219.t1	AT2G38540	63.793	116	36	6	4	115	5	118	2.80e-38	125
MS.gene82219.t1	AT5G59310	60.185	108	38	2	8	115	10	112	1.21e-37	123
MS.gene82219.t1	AT2G38530	61.739	115	40	4	4	115	5	118	1.46e-34	115
MS.gene82219.t1	AT3G51600	53.913	115	49	4	4	115	5	118	2.66e-30	105
MS.gene82219.t1	AT3G51590	47.273	110	55	3	6	114	8	115	7.40e-29	101
MS.gene82219.t1	AT2G15050	45.763	118	58	4	4	115	5	122	3.22e-28	100
MS.gene82219.t1	AT2G15050	45.690	116	57	4	4	113	5	120	8.99e-27	96.3
MS.gene82219.t1	AT2G15050	45.872	109	55	3	4	108	5	113	4.03e-26	94.4
MS.gene82219.t1	AT5G01870	44.737	114	55	4	9	115	4	116	7.80e-26	93.6
MS.gene82219.t1	AT4G33355	48.387	93	48	0	23	115	27	119	3.43e-24	89.7
MS.gene82219.t1	AT4G33355	49.438	89	45	0	23	111	27	115	1.37e-23	88.2
MS.gene82219.t1	AT3G08770	46.392	97	48	3	22	115	18	113	1.16e-21	83.2
MS.gene82219.t1	AT3G08770	46.316	95	47	3	22	113	18	111	9.52e-21	80.9
MS.gene82219.t1	AT2G18370	36.364	110	68	2	6	114	7	115	7.44e-19	75.9
MS.gene82219.t1	AT2G15325	35.165	91	56	2	27	115	31	120	2.26e-12	59.3

Find 31 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTGCTGCTGGTGCTATTTC+TGG	0.136175	4.2:+75519356	MS.gene82219:CDS
CCATGCATTGCATATCTTAG+AGG	0.320070	4.2:+75519233	MS.gene82219:CDS
AATGTTAACACCGCATTTGC+CGG	0.346833	4.2:-75519408	None:intergenic
CCATCTCCAGCATGCTGTGC+TGG	0.375274	4.2:+75519266	MS.gene82219:CDS
AACACAGCTGCTGGTCTTCC+CGG	0.392611	4.2:+75519389	MS.gene82219:CDS
TTGCAAGCAGCCTGACGGTC+AGG	0.421711	4.2:-75519323	None:intergenic
ATCTCATGTGGAACAGTAAC+TGG	0.426922	4.2:+75519200	MS.gene82219:CDS
GGTAGAGGTACTGATCTTGT+AGG	0.430936	4.2:-75519432	None:intergenic
CACCACCACGCCTGACCGTC+AGG	0.443141	4.2:+75519313	MS.gene82219:CDS
ACGGTCAGGCGTGGTGGTGG+CGG	0.445777	4.2:-75519309	None:intergenic
GTCAGGCGTGGTGGTGGCGG+CGG	0.446691	4.2:-75519306	None:intergenic
GTGCATGATTGTTGCACCTA+TGG	0.467928	4.2:+75519166	MS.gene82219:CDS
TTCACTCCAGCACAGCATGC+TGG	0.485039	4.2:-75519272	None:intergenic
AACTGCTTGAAAAGTGCTGC+TGG	0.486030	4.2:+75519344	MS.gene82219:CDS
ATGTTAACACCGCATTTGCC+GGG	0.493990	4.2:-75519407	None:intergenic
GCATATCTTAGAGGTGGTGC+TGG	0.504129	4.2:+75519242	MS.gene82219:CDS
CTGACGGTCAGGCGTGGTGG+TGG	0.517462	4.2:-75519312	None:intergenic
CCAGCACAGCATGCTGGAGA+TGG	0.550113	4.2:-75519266	None:intergenic
TTGAATGCTAACACAGCTGC+TGG	0.551046	4.2:+75519380	MS.gene82219:CDS
GTAGAGGTACTGATCTTGTA+GGG	0.565695	4.2:-75519431	None:intergenic
AGCAGCCTGACGGTCAGGCG+TGG	0.565783	4.2:-75519318	None:intergenic
TGGTTATATTTACAGCATCA+GGG	0.568642	4.2:+75519910	MS.gene82219:intron
AGCCTGACGGTCAGGCGTGG+TGG	0.576862	4.2:-75519315	None:intergenic
GAGATTGCAGCATCTGCCAT+AGG	0.577723	4.2:-75519182	None:intergenic
AAGAACTTACGTAGCACAGT+TGG	0.585095	4.2:-75519453	None:intergenic
CCTCTAAGATATGCAATGCA+TGG	0.588805	4.2:-75519233	None:intergenic
GCTGGTCTTCCCGGCAAATG+CGG	0.592170	4.2:+75519398	MS.gene82219:CDS
TTACGTAGCACAGTTGGTAG+AGG	0.599761	4.2:-75519447	None:intergenic
TGCATTGCATATCTTAGAGG+TGG	0.623803	4.2:+75519236	MS.gene82219:CDS
AGCAGTTGCAAGCAGCCTGA+CGG	0.624471	4.2:-75519328	None:intergenic
GCAGATGCTGCAATCTCATG+TGG	0.654132	4.2:+75519188	MS.gene82219:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATTTTTTTGTGTTATTAT+TGG	+	chr4.2:75519828-75519847	MS.gene82219:intron	10.0%
!!!	AAACATTTTTGTGAATTTTT+TGG	+	chr4.2:75519734-75519753	MS.gene82219:intron	15.0%
!!!	TTTTTCTTACTTCTAAAATT+TGG	-	chr4.2:75519672-75519691	None:intergenic	15.0%
!!!	GACTAGTTTTTTTTTTTGTT+TGG	+	chr4.2:75519890-75519909	MS.gene82219:intron	20.0%
!!!	TTATTGGTTAATATTCAAGT+CGG	+	chr4.2:75519844-75519863	MS.gene82219:intron	20.0%
!	GAAAAATATAGTAAGAGTGA+AGG	-	chr4.2:75519489-75519508	None:intergenic	25.0%
	TGGTTATATTTACAGCATCA+GGG	+	chr4.2:75519910-75519929	MS.gene82219:intron	30.0%
	TTAAGAAACGCTGTATAAAG+AGG	+	chr4.2:75519520-75519539	MS.gene82219:intron	30.0%
	TTGGTTATATTTACAGCATC+AGG	+	chr4.2:75519909-75519928	MS.gene82219:intron	30.0%
	CTGATCTACTGCTTCTTTAT+TGG	+	chr4.2:75519801-75519820	MS.gene82219:intron	35.0%
	AACGCTGTATAAAGAGGTCT+AGG	+	chr4.2:75519526-75519545	MS.gene82219:intron	40.0%
	AAGAACTTACGTAGCACAGT+TGG	-	chr4.2:75519456-75519475	None:intergenic	40.0%
	AATGTTAACACCGCATTTGC+CGG	-	chr4.2:75519411-75519430	None:intergenic	40.0%
	ATCTCATGTGGAACAGTAAC+TGG	+	chr4.2:75519200-75519219	MS.gene82219:CDS	40.0%
	CCATGCATTGCATATCTTAG+AGG	+	chr4.2:75519233-75519252	MS.gene82219:CDS	40.0%
	TATTCAAGTCGGTCGAAAAG+TGG	+	chr4.2:75519855-75519874	MS.gene82219:intron	40.0%
	TGCATTGCATATCTTAGAGG+TGG	+	chr4.2:75519236-75519255	MS.gene82219:CDS	40.0%
!!	CCTCTAAGATATGCAATGCA+TGG	-	chr4.2:75519236-75519255	None:intergenic	40.0%
!!	GTAGAGGTACTGATCTTGTA+GGG	-	chr4.2:75519434-75519453	None:intergenic	40.0%
	ATGTTAACACCGCATTTGCC+GGG	-	chr4.2:75519410-75519429	None:intergenic	45.0%
	GTGCATGATTGTTGCACCTA+TGG	+	chr4.2:75519166-75519185	MS.gene82219:CDS	45.0%
	TTGAATGCTAACACAGCTGC+TGG	+	chr4.2:75519380-75519399	MS.gene82219:CDS	45.0%
!!	AACTGCTTGAAAAGTGCTGC+TGG	+	chr4.2:75519344-75519363	MS.gene82219:CDS	45.0%
!!	GGTAGAGGTACTGATCTTGT+AGG	-	chr4.2:75519435-75519454	None:intergenic	45.0%
!!	TTACGTAGCACAGTTGGTAG+AGG	-	chr4.2:75519450-75519469	None:intergenic	45.0%
!!!	AAAAATTATAATATTTTTAG+AGG	+	chr4.2:75519687-75519706	MS.gene82219:intron	5.0%
!!!	CTTTATAATAATTTTTTTTT+AGG	+	chr4.2:75519763-75519782	MS.gene82219:intron	5.0%
	GAGATTGCAGCATCTGCCAT+AGG	-	chr4.2:75519185-75519204	None:intergenic	50.0%
	GCAGATGCTGCAATCTCATG+TGG	+	chr4.2:75519188-75519207	MS.gene82219:CDS	50.0%
	GCATATCTTAGAGGTGGTGC+TGG	+	chr4.2:75519242-75519261	MS.gene82219:CDS	50.0%
!!	AGTGCTGCTGGTGCTATTTC+TGG	+	chr4.2:75519356-75519375	MS.gene82219:CDS	50.0%
	AGCAGTTGCAAGCAGCCTGA+CGG	-	chr4.2:75519331-75519350	None:intergenic	55.0%
	TTCACTCCAGCACAGCATGC+TGG	-	chr4.2:75519275-75519294	None:intergenic	55.0%
!!	AACACAGCTGCTGGTCTTCC+CGG	+	chr4.2:75519389-75519408	MS.gene82219:CDS	55.0%
	CCAGCACAGCATGCTGGAGA+TGG	-	chr4.2:75519269-75519288	None:intergenic	60.0%
	CCATCTCCAGCATGCTGTGC+TGG	+	chr4.2:75519266-75519285	MS.gene82219:CDS	60.0%
	TTGCAAGCAGCCTGACGGTC+AGG	-	chr4.2:75519326-75519345	None:intergenic	60.0%
!!	GCTGGTCTTCCCGGCAAATG+CGG	+	chr4.2:75519398-75519417	MS.gene82219:CDS	60.0%
	AGCAGCCTGACGGTCAGGCG+TGG	-	chr4.2:75519321-75519340	None:intergenic	70.0%
	AGCCTGACGGTCAGGCGTGG+TGG	-	chr4.2:75519318-75519337	None:intergenic	70.0%
	CACCACCACGCCTGACCGTC+AGG	+	chr4.2:75519313-75519332	MS.gene82219:CDS	70.0%
	CTGACGGTCAGGCGTGGTGG+TGG	-	chr4.2:75519315-75519334	None:intergenic	70.0%
!!	ACGGTCAGGCGTGGTGGTGG+CGG	-	chr4.2:75519312-75519331	None:intergenic	70.0%
!!	GTCAGGCGTGGTGGTGGCGG+CGG	-	chr4.2:75519309-75519328	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.2	gene	75519128	75519937	75519128	ID=MS.gene82219
chr4.2	mRNA	75519128	75519937	75519128	ID=MS.gene82219.t1;Parent=MS.gene82219
chr4.2	exon	75519128	75519465	75519128	ID=MS.gene82219.t1.exon1;Parent=MS.gene82219.t1
chr4.2	CDS	75519128	75519465	75519128	ID=cds.MS.gene82219.t1;Parent=MS.gene82219.t1
chr4.2	exon	75519925	75519937	75519925	ID=MS.gene82219.t1.exon2;Parent=MS.gene82219.t1
chr4.2	CDS	75519925	75519937	75519925	ID=cds.MS.gene82219.t1;Parent=MS.gene82219.t1

Gene Sequence

>MS.gene82219

ATGGCAAGCATGAAGGTAGCATGTGTTGTTTTGATGATGTGCATGATTGTTGCACCTATGGCAGATGCTGCAATCTCATGTGGAACAGTAACTGGTGCTCTTTCTCCATGCATTGCATATCTTAGAGGTGGTGCTGGTCCATCTCCAGCATGCTGTGCTGGAGTGAAGAGACTTAACGCCGCCGCCACCACCACGCCTGACCGTCAGGCTGCTTGCAACTGCTTGAAAAGTGCTGCTGGTGCTATTTCTGGTTTGAATGCTAACACAGCTGCTGGTCTTCCCGGCAAATGCGGTGTTAACATTCCCTACAAGATCAGTACCTCTACCAACTGTGCTACGTAAGTTCTTCTAATTTTTACCTTCACTCTTACTATATTTTTCATGTCAAATATTTAAGAAACGCTGTATAAAGAGGTCTAGGTTGCAAATCTTGACTATTAAAATAACACACTATTAACTGTTTAGAAAAAAAATGAAGCAACTCTATTTAAGCATCACAATAATAAACATACAAAAATTATTAACATAAAAGACTAATTTGCCAAATTTTAGAAGTAAGAAAAATTATAATATTTTTAGAGGATATTTTTATATATTTTTTTAAATAAACATTTTTGTGAATTTTTTGGAATTTACTTTATAATAATTTTTTTTTAGGATTAATTAGTCGCTTCTGATCTACTGCTTCTTTATTGGTTATTTATTTTTTTGTGTTATTATTGGTTAATATTCAAGTCGGTCGAAAAGTGGAATTTAATTATTGACTAGTTTTTTTTTTTGTTTGGTTATATTTACAGCATCAGGGCTTGA

Protein sequence

>MS.gene82219.t1

MASMKVACVVLMMCMIVAPMADAAISCGTVTGALSPCIAYLRGGAGPSPACCAGVKRLNAAATTTPDRQAACNCLKSAAGAISGLNANTAAGLPGKCGVNIPYKISTSTNCATIRA