AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82221


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82221.t1 MTR_4g027800 86.087 115 16 0 3 117 2 116 1.19e-66 197
MS.gene82221.t1 MTR_4g027840 86.842 114 15 0 3 116 2 115 1.41e-66 197
MS.gene82221.t1 MTR_4g428370 82.609 115 20 0 3 117 59 173 1.59e-64 193
MS.gene82221.t1 MTR_4g029390 64.655 116 38 2 3 116 2 116 2.08e-46 145
MS.gene82221.t1 MTR_4g029350 64.655 116 38 2 3 116 2 116 2.82e-45 142
MS.gene82221.t1 MTR_4g029210 50.000 110 55 0 4 113 3 112 7.01e-32 108
MS.gene82221.t1 MTR_7g072930 49.153 118 55 3 1 114 1 117 1.57e-31 109
MS.gene82221.t1 MTR_4g028190 48.305 118 56 3 1 113 1 118 1.71e-30 105
MS.gene82221.t1 MTR_7g073150 47.458 118 57 4 1 114 1 117 2.90e-29 102
MS.gene82221.t1 MTR_7g072830 40.833 120 67 2 1 116 1 120 1.70e-27 98.2
MS.gene82221.t1 MTR_7g073170 44.538 119 60 4 1 115 1 117 4.10e-27 97.1
MS.gene82221.t1 MTR_7g072730 40.678 118 66 3 1 114 1 118 8.60e-26 95.1
MS.gene82221.t1 MTR_4g028180 40.678 118 63 3 6 116 9 126 5.79e-25 91.7
MS.gene82221.t1 MTR_4g028310 38.983 118 69 1 3 117 2 119 2.57e-24 90.5
MS.gene82221.t1 MTR_2g062600 42.241 116 64 3 1 115 1 114 6.67e-24 88.6
MS.gene82221.t1 MTR_7g073120 40.171 117 65 2 1 112 1 117 8.06e-23 87.0
MS.gene82221.t1 MTR_7g072900 46.610 118 56 5 1 113 1 116 1.29e-22 85.5
MS.gene82221.t1 MTR_7g072993 45.763 118 57 4 1 112 1 117 2.04e-22 85.5
MS.gene82221.t1 MTR_7g072960 41.880 117 51 4 1 114 1 103 6.44e-21 81.6
MS.gene82221.t1 MTR_4g028250 39.316 117 68 2 3 116 2 118 5.03e-20 79.0
MS.gene82221.t1 MTR_3g467100 36.607 112 70 1 6 116 3 114 7.08e-20 78.6
MS.gene82221.t1 MTR_7g072980 40.678 118 64 4 1 113 1 117 2.05e-19 78.2
MS.gene82221.t1 MTR_1g040335 43.697 119 62 3 1 115 1 118 7.60e-19 75.9
MS.gene82221.t1 MTR_7g073130 41.026 117 63 5 1 113 1 115 1.39e-18 75.5
MS.gene82221.t1 MTR_7g073100 40.336 119 62 3 1 113 1 116 4.47e-18 74.7
MS.gene82221.t1 MTR_7g073060 40.000 120 67 4 1 116 1 119 1.26e-17 72.8
MS.gene82221.t1 MTR_7g451500 36.957 92 57 1 25 115 7 98 5.16e-17 70.9
MS.gene82221.t1 MTR_4g028360 35.398 113 70 3 5 115 1 112 1.72e-16 70.1
MS.gene82221.t1 MTR_7g072810 41.379 116 58 4 1 112 1 110 3.06e-16 69.3
MS.gene82221.t1 MTR_7g073030 37.069 116 68 4 1 112 1 115 1.42e-14 65.9
MS.gene82221.t1 MTR_3g056640 35.398 113 65 4 10 116 12 122 5.90e-14 63.5
MS.gene82221.t1 MTR_3g046515 34.821 112 67 4 4 111 10 119 3.08e-13 61.6
MS.gene82221.t1 MTR_1g040330 40.496 121 65 5 1 116 1 119 5.87e-13 60.8
MS.gene82221.t1 MTR_3g046530 31.356 118 73 4 5 116 7 122 7.45e-13 60.8
MS.gene82221.t1 MTR_3g464130 37.778 90 51 3 26 111 18 106 2.60e-12 58.9
MS.gene82221.t1 MTR_3g046540 33.043 115 67 5 5 111 7 119 5.02e-12 58.5
MS.gene82221.t1 MTR_7g072760 31.967 122 77 3 1 116 1 122 6.85e-12 58.2
MS.gene82221.t1 MTR_3g464110 40.244 82 44 3 34 111 92 172 3.03e-11 57.4
MS.gene82221.t1 MTR_7g072987 47.059 68 33 2 26 91 57 123 7.95e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82221.t1 AT2G38540 61.667 120 40 6 1 116 1 118 4.35e-38 124
MS.gene82221.t1 AT5G59320 56.410 117 48 2 1 116 1 115 4.74e-38 124
MS.gene82221.t1 AT5G59310 56.897 116 46 2 1 116 1 112 4.92e-37 122
MS.gene82221.t1 AT2G38530 59.664 119 44 4 1 116 1 118 1.11e-34 116
MS.gene82221.t1 AT3G51600 51.261 119 54 4 1 116 1 118 4.09e-30 104
MS.gene82221.t1 AT3G51590 44.828 116 62 2 1 115 1 115 7.83e-28 99.0
MS.gene82221.t1 AT2G15050 43.443 122 63 4 1 116 1 122 1.38e-27 98.6
MS.gene82221.t1 AT2G15050 43.333 120 62 4 1 114 1 120 3.84e-26 94.7
MS.gene82221.t1 AT4G33355 42.982 114 64 1 3 116 7 119 1.30e-25 93.2
MS.gene82221.t1 AT2G15050 43.363 113 60 3 1 109 1 113 2.50e-25 92.4
MS.gene82221.t1 AT5G01870 43.860 114 56 4 10 116 4 116 4.83e-25 91.7
MS.gene82221.t1 AT4G33355 43.636 110 61 1 3 112 7 115 5.29e-25 91.7
MS.gene82221.t1 AT3G08770 45.361 97 49 3 23 116 18 113 3.72e-20 79.3
MS.gene82221.t1 AT3G08770 46.237 93 46 3 23 112 18 109 3.46e-19 77.0
MS.gene82221.t1 AT2G18370 36.364 110 68 2 7 115 7 115 5.53e-18 73.6
MS.gene82221.t1 AT2G15325 29.752 121 79 3 1 116 1 120 9.88e-13 60.5

Find 32 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGCTGCTGGTGCTATTTC+TGG 0.136175 4.2:+75490141 MS.gene82221:CDS
CCATGCATTGCATATCTTAG+AGG 0.320070 4.2:+75490018 MS.gene82221:CDS
GGCGCAGCTTCTTCTCTTCC+TGG 0.339122 4.2:+75490174 MS.gene82221:CDS
GCTATTTCTGGTTTGAATAC+AGG 0.359485 4.2:+75490153 MS.gene82221:CDS
TTGCAAGCAGCCTGGCGGTC+AGG 0.369068 4.2:-75490108 None:intergenic
CCATCTCCAGCATGCTGTGC+TGG 0.375274 4.2:+75490051 MS.gene82221:CDS
GTCAGGCGTGGTGGTGGCGG+CGG 0.430334 4.2:-75490091 None:intergenic
GCGGTCAGGCGTGGTGGTGG+CGG 0.445777 4.2:-75490094 None:intergenic
CTGGCGGTCAGGCGTGGTGG+TGG 0.447269 4.2:-75490097 None:intergenic
CACCACCACGCCTGACCGCC+AGG 0.460254 4.2:+75490098 MS.gene82221:CDS
GTGCATGATTGTTGCACCTA+TGG 0.467928 4.2:+75489951 MS.gene82221:CDS
AGCAGCCTGGCGGTCAGGCG+TGG 0.477818 4.2:-75490103 None:intergenic
TTCACTCCAGCACAGCATGC+TGG 0.485039 4.2:-75490057 None:intergenic
AACTGCTTGAAAAGTGCTGC+TGG 0.486030 4.2:+75490129 MS.gene82221:CDS
TCAAGCAGTTGCAAGCAGCC+TGG 0.486116 4.2:-75490116 None:intergenic
ATGTTAACGCCACATTTGCC+AGG 0.486943 4.2:-75490192 None:intergenic
GCATATCTTAGAGGTGGTGC+TGG 0.504129 4.2:+75490027 MS.gene82221:CDS
GTGGAGGTACTGATCTTGTA+AGG 0.508470 4.2:-75490216 None:intergenic
TTGTCATGCTTACAGCATCA+GGG 0.542932 4.2:+75490618 MS.gene82221:intron
CCAGCACAGCATGCTGGAGA+TGG 0.550113 4.2:-75490051 None:intergenic
AACATACGTAGCACAGTTGG+TGG 0.554041 4.2:-75490235 None:intergenic
AGCCTGGCGGTCAGGCGTGG+TGG 0.562191 4.2:-75490100 None:intergenic
AACAATGGCAAGCAGCATGA+AGG 0.575179 4.2:+75489906 None:intergenic
GAGATTGCAGCATCTGCCAT+AGG 0.577723 4.2:-75489967 None:intergenic
TCTTCTCTTCCTGGCAAATG+TGG 0.581280 4.2:+75490183 MS.gene82221:CDS
CCTCTAAGATATGCAATGCA+TGG 0.588805 4.2:-75490018 None:intergenic
ATAAACATACGTAGCACAGT+TGG 0.615719 4.2:-75490238 None:intergenic
TGCATTGCATATCTTAGAGG+TGG 0.623803 4.2:+75490021 MS.gene82221:CDS
ATACGTAGCACAGTTGGTGG+AGG 0.639269 4.2:-75490232 None:intergenic
AGCAGTTGCAAGCAGCCTGG+CGG 0.651296 4.2:-75490113 None:intergenic
GCAGATGCTGCAATCTCATG+TGG 0.654132 4.2:+75489973 MS.gene82221:CDS
ATCTCATGTGGACAAGTGAG+TGG 0.724614 4.2:+75489985 MS.gene82221:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATCATTCTTTTAATTTT+AGG + chr4.2:75490378-75490397 MS.gene82221:intron 10.0%
!!! TTATATTATTCAATGTTTTT+GGG + chr4.2:75490507-75490526 MS.gene82221:intron 10.0%
!!! TTTATATTATTCAATGTTTT+TGG + chr4.2:75490506-75490525 MS.gene82221:intron 10.0%
!!! AAAATTTAGTTGTTTAACTT+TGG - chr4.2:75490335-75490354 None:intergenic 15.0%
!!! TATAAATTCATTTTTGCTGA+AGG + chr4.2:75490451-75490470 MS.gene82221:intron 20.0%
!! TAGAAAATTAATCACTGACT+AGG + chr4.2:75490583-75490602 MS.gene82221:intron 25.0%
!!! CTACGAATCTACTTTTTTAT+TGG + chr4.2:75490551-75490570 MS.gene82221:intron 25.0%
!!! TGGGAATTTTTTTGAGATTA+AGG + chr4.2:75490526-75490545 MS.gene82221:intron 25.0%
!!! TTTTATCCTCATTTTTCAAG+TGG + chr4.2:75490281-75490300 MS.gene82221:intron 25.0%
! TTACCATTTGAGATTGTTGA+GGG + chr4.2:75490355-75490374 MS.gene82221:intron 30.0%
! TTTACCATTTGAGATTGTTG+AGG + chr4.2:75490354-75490373 MS.gene82221:intron 30.0%
ACTAGACCACTTGAAAAATG+AGG - chr4.2:75490290-75490309 None:intergenic 35.0%
ATAAACATACGTAGCACAGT+TGG - chr4.2:75490241-75490260 None:intergenic 35.0%
TTACCCTCAACAATCTCAAA+TGG - chr4.2:75490361-75490380 None:intergenic 35.0%
!! GCTATTTCTGGTTTGAATAC+AGG + chr4.2:75490153-75490172 MS.gene82221:CDS 35.0%
CCATGCATTGCATATCTTAG+AGG + chr4.2:75490018-75490037 MS.gene82221:CDS 40.0%
TGCATTGCATATCTTAGAGG+TGG + chr4.2:75490021-75490040 MS.gene82221:CDS 40.0%
TTGTCATGCTTACAGCATCA+GGG + chr4.2:75490618-75490637 MS.gene82221:intron 40.0%
TTTGTCATGCTTACAGCATC+AGG + chr4.2:75490617-75490636 MS.gene82221:intron 40.0%
!! CCTCTAAGATATGCAATGCA+TGG - chr4.2:75490021-75490040 None:intergenic 40.0%
AACATACGTAGCACAGTTGG+TGG - chr4.2:75490238-75490257 None:intergenic 45.0%
ATCTCATGTGGACAAGTGAG+TGG + chr4.2:75489985-75490004 MS.gene82221:CDS 45.0%
ATGTTAACGCCACATTTGCC+AGG - chr4.2:75490195-75490214 None:intergenic 45.0%
GTGCATGATTGTTGCACCTA+TGG + chr4.2:75489951-75489970 MS.gene82221:CDS 45.0%
TCTTCTCTTCCTGGCAAATG+TGG + chr4.2:75490183-75490202 MS.gene82221:CDS 45.0%
!! AACTGCTTGAAAAGTGCTGC+TGG + chr4.2:75490129-75490148 MS.gene82221:CDS 45.0%
!! GTGGAGGTACTGATCTTGTA+AGG - chr4.2:75490219-75490238 None:intergenic 45.0%
GAGATTGCAGCATCTGCCAT+AGG - chr4.2:75489970-75489989 None:intergenic 50.0%
GCAGATGCTGCAATCTCATG+TGG + chr4.2:75489973-75489992 MS.gene82221:CDS 50.0%
GCATATCTTAGAGGTGGTGC+TGG + chr4.2:75490027-75490046 MS.gene82221:CDS 50.0%
!! AGTGCTGCTGGTGCTATTTC+TGG + chr4.2:75490141-75490160 MS.gene82221:CDS 50.0%
!! ATACGTAGCACAGTTGGTGG+AGG - chr4.2:75490235-75490254 None:intergenic 50.0%
TCAAGCAGTTGCAAGCAGCC+TGG - chr4.2:75490119-75490138 None:intergenic 55.0%
TTCACTCCAGCACAGCATGC+TGG - chr4.2:75490060-75490079 None:intergenic 55.0%
AGCAGTTGCAAGCAGCCTGG+CGG - chr4.2:75490116-75490135 None:intergenic 60.0%
CCAGCACAGCATGCTGGAGA+TGG - chr4.2:75490054-75490073 None:intergenic 60.0%
CCATCTCCAGCATGCTGTGC+TGG + chr4.2:75490051-75490070 MS.gene82221:CDS 60.0%
GGCGCAGCTTCTTCTCTTCC+TGG + chr4.2:75490174-75490193 MS.gene82221:CDS 60.0%
TTGCAAGCAGCCTGGCGGTC+AGG - chr4.2:75490111-75490130 None:intergenic 65.0%
AGCAGCCTGGCGGTCAGGCG+TGG - chr4.2:75490106-75490125 None:intergenic 75.0%
AGCCTGGCGGTCAGGCGTGG+TGG - chr4.2:75490103-75490122 None:intergenic 75.0%
CACCACCACGCCTGACCGCC+AGG + chr4.2:75490098-75490117 MS.gene82221:CDS 75.0%
CTGGCGGTCAGGCGTGGTGG+TGG - chr4.2:75490100-75490119 None:intergenic 75.0%
!! GCGGTCAGGCGTGGTGGTGG+CGG - chr4.2:75490097-75490116 None:intergenic 75.0%
!! GTCAGGCGTGGTGGTGGCGG+CGG - chr4.2:75490094-75490113 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 75489910 75490645 75489910 ID=MS.gene82221
chr4.2 mRNA 75489910 75490645 75489910 ID=MS.gene82221.t1;Parent=MS.gene82221
chr4.2 exon 75489910 75490250 75489910 ID=MS.gene82221.t1.exon1;Parent=MS.gene82221.t1
chr4.2 CDS 75489910 75490250 75489910 ID=cds.MS.gene82221.t1;Parent=MS.gene82221.t1
chr4.2 exon 75490633 75490645 75490633 ID=MS.gene82221.t1.exon2;Parent=MS.gene82221.t1
chr4.2 CDS 75490633 75490645 75490633 ID=cds.MS.gene82221.t1;Parent=MS.gene82221.t1
Gene Sequence

>MS.gene82221

ATGGCAAGCAGCATGAAGGTAGCATGTGTTGTTTTGATGATGTGCATGATTGTTGCACCTATGGCAGATGCTGCAATCTCATGTGGACAAGTGAGTGGTGCTCTTTCTCCATGCATTGCATATCTTAGAGGTGGTGCTGGTCCATCTCCAGCATGCTGTGCTGGAGTGAAGAGACTTAACGCCGCCGCCACCACCACGCCTGACCGCCAGGCTGCTTGCAACTGCTTGAAAAGTGCTGCTGGTGCTATTTCTGGTTTGAATACAGGCGCAGCTTCTTCTCTTCCTGGCAAATGTGGCGTTAACATTCCTTACAAGATCAGTACCTCCACCAACTGTGCTACGTATGTTTATCTAACTTCAAACTTTCACTTTTTTATCCTCATTTTTCAAGTGGTCTAGTATTCATAACATAACAGAAGTAACCAAAGTTAAACAACTAAATTTTTTACCATTTGAGATTGTTGAGGGTAAATCATTCTTTTAATTTTAGGATCACTTTATAGATATGCTATTTTATTTTCTATAGTACGTAGTAAAATTTTATAAATTCATTTTTGCTGAAGGATGTTTTAATATCATATTTTGTTAATAAAGATTTTATATTATTCAATGTTTTTGGGAATTTTTTTGAGATTAAGGTACTACGAATCTACTTTTTTATTGGTCTATAAAATAGAAAATTAATCACTGACTAGGTTTTTTTTTTTTTTGTCATGCTTACAGCATCAGGGCTTGA

Protein sequence

>MS.gene82221.t1

MASSMKVACVVLMMCMIVAPMADAAISCGQVSGALSPCIAYLRGGAGPSPACCAGVKRLNAAATTTPDRQAACNCLKSAAGAISGLNTGAASSLPGKCGVNIPYKISTSTNCATIRA