Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77325.t1 | XP_003623278.1 | 91.4 | 220 | 18 | 1 | 353 | 571 | 1 | 220 | 1.40E-103 | 387.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77325.t1 | G7KRF4 | 91.4 | 220 | 18 | 1 | 353 | 571 | 1 | 220 | 1.0e-103 | 387.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04925 | MS.gene77325 | 0.814982 | 1.17E-51 | -1.69E-46 |
| MS.gene04926 | MS.gene77325 | 0.803402 | 3.55E-49 | -1.69E-46 |
| MS.gene049549 | MS.gene77325 | 0.827823 | 1.26E-54 | -1.69E-46 |
| MS.gene050720 | MS.gene77325 | 0.801231 | 9.93E-49 | -1.69E-46 |
| MS.gene051996 | MS.gene77325 | 0.814534 | 1.47E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene77325 | 0.82426 | 8.89E-54 | -1.69E-46 |
| MS.gene05240 | MS.gene77325 | 0.851036 | 1.15E-60 | -1.69E-46 |
| MS.gene052606 | MS.gene77325 | 0.802523 | 5.39E-49 | -1.69E-46 |
| MS.gene053406 | MS.gene77325 | 0.831967 | 1.24E-55 | -1.69E-46 |
| MS.gene053887 | MS.gene77325 | 0.805557 | 1.26E-49 | -1.69E-46 |
| MS.gene054277 | MS.gene77325 | 0.841425 | 4.79E-58 | -1.69E-46 |
| MS.gene054421 | MS.gene77325 | 0.809357 | 1.97E-50 | -1.69E-46 |
| MS.gene054742 | MS.gene77325 | 0.872812 | 2.39E-67 | -1.69E-46 |
| MS.gene05483 | MS.gene77325 | 0.807693 | 4.46E-50 | -1.69E-46 |
| MS.gene054884 | MS.gene77325 | 0.818544 | 1.85E-52 | -1.69E-46 |
| MS.gene055240 | MS.gene77325 | 0.819415 | 1.18E-52 | -1.69E-46 |
| MS.gene056330 | MS.gene77325 | 0.812469 | 4.17E-51 | -1.69E-46 |
| MS.gene056590 | MS.gene77325 | 0.805175 | 1.52E-49 | -1.69E-46 |
| MS.gene056591 | MS.gene77325 | 0.805894 | 1.07E-49 | -1.69E-46 |
| MS.gene056680 | MS.gene77325 | 0.842678 | 2.23E-58 | -1.69E-46 |
| MS.gene056748 | MS.gene77325 | 0.816468 | 5.45E-52 | -1.69E-46 |
| MS.gene056920 | MS.gene77325 | 0.880554 | 4.99E-70 | -1.69E-46 |
| MS.gene056922 | MS.gene77325 | 0.819081 | 1.40E-52 | -1.69E-46 |
| MS.gene057261 | MS.gene77325 | 0.811284 | 7.56E-51 | -1.69E-46 |
| MS.gene057544 | MS.gene77325 | 0.809708 | 1.66E-50 | -1.69E-46 |
| MS.gene058450 | MS.gene77325 | 0.803044 | 4.21E-49 | -1.69E-46 |
| MS.gene058603 | MS.gene77325 | 0.818714 | 1.70E-52 | -1.69E-46 |
| MS.gene059891 | MS.gene77325 | 0.817436 | 3.30E-52 | -1.69E-46 |
| MS.gene059892 | MS.gene77325 | 0.821643 | 3.62E-53 | -1.69E-46 |
| MS.gene059953 | MS.gene77325 | 0.812105 | 5.01E-51 | -1.69E-46 |
| MS.gene059955 | MS.gene77325 | 0.83315 | 6.29E-56 | -1.69E-46 |
| MS.gene060087 | MS.gene77325 | 0.878856 | 2.00E-69 | -1.69E-46 |
| MS.gene061038 | MS.gene77325 | 0.815302 | 9.91E-52 | -1.69E-46 |
| MS.gene061092 | MS.gene77325 | 0.807966 | 3.90E-50 | -1.69E-46 |
| MS.gene061094 | MS.gene77325 | 0.816694 | 4.85E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77325.t1 | MTR_7g068110 | 91.364 | 220 | 18 | 1 | 353 | 571 | 1 | 220 | 2.56e-140 | 405 |
| MS.gene77325.t1 | MTR_7g068140 | 53.070 | 228 | 93 | 6 | 353 | 571 | 1 | 223 | 1.82e-62 | 205 |
| MS.gene77325.t1 | MTR_3g062340 | 27.742 | 155 | 110 | 1 | 415 | 569 | 1795 | 1947 | 1.89e-11 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 120 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGATTTCTTAAACGAATT+TGG | 0.095942 | 7.2:+35630159 | None:intergenic |
| CACGTACCAATAATTGTTTC+TGG | 0.192780 | 7.2:+35623747 | None:intergenic |
| ATGCGACTCTAAATATTAAA+AGG | 0.195749 | 7.2:+35621943 | None:intergenic |
| TTGTCACAAAAGATTGTTAT+TGG | 0.203311 | 7.2:+35627060 | None:intergenic |
| AATTAGTATACTTGGCTTCT+TGG | 0.241814 | 7.2:+35622118 | None:intergenic |
| CCAAATTCGTTTAAGAAATC+AGG | 0.277660 | 7.2:-35630159 | MS.gene77325:CDS |
| CAGTGTTCTTTCCCTTGGAC+TGG | 0.279150 | 7.2:+35629918 | None:intergenic |
| CAGGTTTCGCAGCTCTTCTT+TGG | 0.288928 | 7.2:+35622146 | None:intergenic |
| GTGTGTTTCTAACCGTCTTC+TGG | 0.296829 | 7.2:+35623803 | None:intergenic |
| ACCCTCAGTGTTCTTTCCCT+TGG | 0.317899 | 7.2:+35629913 | None:intergenic |
| ACACTCGCCTGCACTCTCTC+TGG | 0.323298 | 7.2:+35631756 | None:intergenic |
| CCCTTGGACTGGGCTTTCTC+AGG | 0.327284 | 7.2:+35629929 | None:intergenic |
| GGATATCGATTCTACTGTTC+TGG | 0.336928 | 7.2:-35628761 | MS.gene77325:CDS |
| TAATGTAGTCATCAAGTCTA+AGG | 0.337618 | 7.2:+35622275 | None:intergenic |
| CAAGCCCCAGAAACAATTAT+TGG | 0.339566 | 7.2:-35623753 | MS.gene77325:intron |
| CGATATCCCTTAGCACGTTC+TGG | 0.343279 | 7.2:+35628776 | None:intergenic |
| CCAGTTATTCTTGCCAAAAC+GGG | 0.344534 | 7.2:+35630272 | None:intergenic |
| CTCGATATTATTAATATGTA+TGG | 0.344554 | 7.2:-35622231 | MS.gene77325:CDS |
| GTCCATTCCAAACCCCGTTT+TGG | 0.351451 | 7.2:-35630285 | MS.gene77325:CDS |
| ATTCTTGCCAAAACGGGGTT+TGG | 0.352736 | 7.2:+35630278 | None:intergenic |
| CTATGGACTGCGTTTCTAAT+AGG | 0.356507 | 7.2:-35622005 | MS.gene77325:CDS |
| TTCTTAAACGAATTTGGATT+AGG | 0.357923 | 7.2:+35630165 | None:intergenic |
| TATACTAATTCGAAACTTGT+TGG | 0.366713 | 7.2:-35622105 | MS.gene77325:CDS |
| CTGAGGAGTCGCGCATCATT+AGG | 0.369116 | 7.2:+35622417 | None:intergenic |
| AGTGTTCTTTCCCTTGGACT+GGG | 0.386836 | 7.2:+35629919 | None:intergenic |
| ACGATCTCCGAGCATCTTCT+TGG | 0.391951 | 7.2:-35630674 | MS.gene77325:CDS |
| GTTTCTGACAATGCTGAATC+TGG | 0.396650 | 7.2:-35630590 | MS.gene77325:CDS |
| TCCAGTTATTCTTGCCAAAA+CGG | 0.399692 | 7.2:+35630271 | None:intergenic |
| ACTTGGCTTCTTGGTCAGTC+AGG | 0.405311 | 7.2:+35622127 | None:intergenic |
| ACGTACCAATAATTGTTTCT+GGG | 0.415337 | 7.2:+35623748 | None:intergenic |
| TTCGAAACTTGTTGGAGAGT+TGG | 0.417533 | 7.2:-35622097 | MS.gene77325:CDS |
| GATTGTTATTGGCTTATCAA+TGG | 0.420680 | 7.2:+35627071 | None:intergenic |
| CACTCTCTCTGGGTGAGAGT+GGG | 0.421536 | 7.2:+35631767 | None:intergenic |
| AAGAATATCAAAACTTCTAG+TGG | 0.424809 | 7.2:-35627015 | MS.gene77325:CDS |
| TAATAGGAAGAAGCAAGTTA+AGG | 0.433078 | 7.2:-35621989 | MS.gene77325:CDS |
| GGATCTAATTTCCTTTGAAA+AGG | 0.435139 | 7.2:+35628830 | None:intergenic |
| CGCATCATTAGGAGGGAAAT+CGG | 0.436259 | 7.2:+35622428 | None:intergenic |
| TTCTAGTGGGTCGAAGCACT+TGG | 0.458338 | 7.2:-35627001 | MS.gene77325:CDS |
| GCACTCTCTCTGGGTGAGAG+TGG | 0.461888 | 7.2:+35631766 | None:intergenic |
| TAAGCCTAATTGCTATCAAA+AGG | 0.462267 | 7.2:-35630697 | MS.gene77325:CDS |
| AAGTTTCGAATTAGTATACT+TGG | 0.462570 | 7.2:+35622110 | None:intergenic |
| CTAATGATGCGCGACTCCTC+AGG | 0.467252 | 7.2:-35622416 | MS.gene77325:CDS |
| ATCTCCGAGCATCTTCTTGG+TGG | 0.470040 | 7.2:-35630671 | MS.gene77325:CDS |
| CGTTAGAGATCTTGTTAAAG+AGG | 0.473458 | 7.2:-35626962 | MS.gene77325:CDS |
| TGCGACTCTAAATATTAAAA+GGG | 0.473619 | 7.2:+35621944 | None:intergenic |
| TCCTTAGCAGTGCACTCCTC+AGG | 0.477608 | 7.2:+35630644 | None:intergenic |
| TGCCAAAACGGGGTTTGGAA+TGG | 0.487127 | 7.2:+35630283 | None:intergenic |
| ACAGGTCATAACAACTTTGT+AGG | 0.490462 | 7.2:-35630192 | MS.gene77325:CDS |
| CACTCGCCTGCACTCTCTCT+GGG | 0.491435 | 7.2:+35631757 | None:intergenic |
| TCATGCTTCTCACAAAGGTC+AGG | 0.492769 | 7.2:-35630232 | MS.gene77325:CDS |
| CTCTTTAACAAGATCTCTAA+CGG | 0.492948 | 7.2:+35626963 | None:intergenic |
| GAAACTTGTTGGAGAGTTGG+AGG | 0.505875 | 7.2:-35622094 | MS.gene77325:CDS |
| CGATTCTACTGTTCTGGAGG+TGG | 0.506636 | 7.2:-35628755 | MS.gene77325:CDS |
| ATTAATATGTATGGCTTCAG+AGG | 0.516211 | 7.2:-35622222 | MS.gene77325:CDS |
| CTAAGTGGGTGAGAGCTGAC+AGG | 0.520057 | 7.2:-35630721 | MS.gene77325:CDS |
| TGGATATCCAGAACGTGCTA+AGG | 0.521721 | 7.2:-35628783 | MS.gene77325:CDS |
| AAAACATTACGCCGAGGTGC+AGG | 0.528899 | 7.2:+35622468 | None:intergenic |
| TCGCAAGAGCTTGAGGAAGT+TGG | 0.534058 | 7.2:+35622308 | None:intergenic |
| GCCTGAGGAGTGCACTGCTA+AGG | 0.534788 | 7.2:-35630645 | MS.gene77325:CDS |
| TGGTTAAAGAATCTAGTGAC+AGG | 0.536565 | 7.2:-35627111 | MS.gene77325:intron |
| AGAGACAAACTCACCGATGA+TGG | 0.537336 | 7.2:+35628641 | None:intergenic |
| TGTTAAAGAGGGAAATATAG+TGG | 0.538655 | 7.2:-35626950 | MS.gene77325:intron |
| GTTAGAGATCTTGTTAAAGA+GGG | 0.545850 | 7.2:-35626961 | MS.gene77325:CDS |
| TCTTCTTGGTGGTTTGCCTG+AGG | 0.546727 | 7.2:-35630660 | MS.gene77325:CDS |
| AGCTCCACGTCTTATGTCAA+GGG | 0.547099 | 7.2:+35622344 | None:intergenic |
| CAACACTCGTCCACCATCAT+CGG | 0.549590 | 7.2:-35628654 | MS.gene77325:intron |
| TCCTTGATGCTTTAAAAGAG+AGG | 0.550493 | 7.2:-35630619 | MS.gene77325:CDS |
| TTCTTTGGCAATATCAAGAG+TGG | 0.554959 | 7.2:+35622161 | None:intergenic |
| GAGAGAACGTCTTGCTAAGT+GGG | 0.556564 | 7.2:-35630735 | MS.gene77325:CDS |
| TGCTAAATGTGTAGCTGAGG+AGG | 0.559012 | 7.2:-35630349 | MS.gene77325:intron |
| AGAATATCAAAACTTCTAGT+GGG | 0.559174 | 7.2:-35627014 | MS.gene77325:CDS |
| AAGCTCCACGTCTTATGTCA+AGG | 0.560627 | 7.2:+35622343 | None:intergenic |
| ACCTGAGAAAGCCCAGTCCA+AGG | 0.560655 | 7.2:-35629930 | MS.gene77325:CDS |
| GAAAATAAGCAAAAGTCTGA+AGG | 0.562929 | 7.2:-35630069 | MS.gene77325:intron |
| AAGTTGTTATGACCTGTGCC+TGG | 0.565786 | 7.2:+35630198 | None:intergenic |
| CGTACCAATAATTGTTTCTG+GGG | 0.566692 | 7.2:+35623749 | None:intergenic |
| TAATGATGCGCGACTCCTCA+GGG | 0.567168 | 7.2:-35622415 | MS.gene77325:CDS |
| AGGAGTCGCGCATCATTAGG+AGG | 0.568050 | 7.2:+35622420 | None:intergenic |
| CTCTCACCCAGAGAGAGTGC+AGG | 0.569919 | 7.2:-35631763 | MS.gene77325:CDS |
| TCTTTACAGGTCCTGCACCT+CGG | 0.572942 | 7.2:-35622479 | MS.gene77325:intron |
| CCTGAGAAAGCCCAGTCCAA+GGG | 0.575938 | 7.2:-35629929 | MS.gene77325:CDS |
| AGAGAGAACGTCTTGCTAAG+TGG | 0.578047 | 7.2:-35630736 | MS.gene77325:CDS |
| GCAAAATATGATAATGATGA+GGG | 0.581606 | 7.2:-35630542 | MS.gene77325:intron |
| TTAAAATCACATGTTGCTGA+AGG | 0.586212 | 7.2:-35622255 | MS.gene77325:CDS |
| TTGGCAATATCAAGAGTGGA+GGG | 0.586325 | 7.2:+35622165 | None:intergenic |
| CAGTTATTCTTGCCAAAACG+GGG | 0.587836 | 7.2:+35630273 | None:intergenic |
| ACTTGTTGGAGAGTTGGAGG+AGG | 0.591965 | 7.2:-35622091 | MS.gene77325:CDS |
| TTTGGCAATATCAAGAGTGG+AGG | 0.592538 | 7.2:+35622164 | None:intergenic |
| GAATCGAATTCGGACCTACA+GGG | 0.597674 | 7.2:+35631728 | None:intergenic |
| TAGCGACGAGAAACTAGCTA+TGG | 0.597878 | 7.2:-35622022 | MS.gene77325:CDS |
| TGCAAAATATGATAATGATG+AGG | 0.600556 | 7.2:-35630543 | MS.gene77325:CDS |
| TCATCTCATGCTTCTCACAA+AGG | 0.602502 | 7.2:-35630237 | MS.gene77325:CDS |
| GGATATCCAGAACGTGCTAA+GGG | 0.603246 | 7.2:-35628782 | MS.gene77325:CDS |
| CAAACCACCAAGAAGATGCT+CGG | 0.606964 | 7.2:+35630667 | None:intergenic |
| ATTCGTTTAAGAAATCAGGT+TGG | 0.610447 | 7.2:-35630155 | MS.gene77325:CDS |
| AGTGTGATCGCAAGAGCTTG+AGG | 0.610597 | 7.2:+35622301 | None:intergenic |
| AAGATGTTCATCTCGACCAT+CGG | 0.612616 | 7.2:+35623842 | None:intergenic |
| TTTGGAATGGACAGACAGCA+AGG | 0.626108 | 7.2:+35630296 | None:intergenic |
| TCCAAGGGAAAGAACACTGA+GGG | 0.626616 | 7.2:-35629914 | MS.gene77325:intron |
| ATACTCTCAGCATTGACCAC+AGG | 0.628267 | 7.2:+35630767 | None:intergenic |
| GGTCAGGATGTTAAGAAACC+AGG | 0.629757 | 7.2:-35630216 | MS.gene77325:CDS |
| GGCGAGTGTGACCTCCCTGT+AGG | 0.632534 | 7.2:-35631742 | MS.gene77325:intron |
| TGTATGGCTTCAGAGGTGAA+TGG | 0.635609 | 7.2:-35622215 | MS.gene77325:CDS |
| GGTCATAACAACTTTGTAGG+AGG | 0.635625 | 7.2:-35630189 | MS.gene77325:CDS |
| TATCGATTCTACTGTTCTGG+AGG | 0.636063 | 7.2:-35628758 | MS.gene77325:CDS |
| GATGTTAAGAAACCAGGCAC+AGG | 0.638549 | 7.2:-35630210 | MS.gene77325:CDS |
| GACAAACTCACCGATGATGG+TGG | 0.638983 | 7.2:+35628644 | None:intergenic |
| CTTAGACATAGCATTGCGCA+AGG | 0.647618 | 7.2:-35630795 | MS.gene77325:intron |
| CATCCCCTTGACATAAGACG+TGG | 0.651438 | 7.2:-35622348 | MS.gene77325:CDS |
| ATGTTCATCTCGACCATCGG+TGG | 0.663599 | 7.2:+35623845 | None:intergenic |
| AAATCGGCAACAACAGAGCG+TGG | 0.663909 | 7.2:+35622444 | None:intergenic |
| GCTCCACGTCTTATGTCAAG+GGG | 0.665557 | 7.2:+35622345 | None:intergenic |
| CACAACGAGCTTCAGCGCAG+AGG | 0.671568 | 7.2:-35623783 | MS.gene77325:CDS |
| GGAGTCGCGCATCATTAGGA+GGG | 0.672305 | 7.2:+35622421 | None:intergenic |
| ACAGTAGAAGAACCAGAAGA+CGG | 0.678706 | 7.2:-35623815 | MS.gene77325:CDS |
| ATTGCGCAAGGATAAGCCTG+TGG | 0.709585 | 7.2:-35630783 | MS.gene77325:CDS |
| CGTGGTAAAACATTACGCCG+AGG | 0.728823 | 7.2:+35622462 | None:intergenic |
| TCGAATTCGGACCTACAGGG+AGG | 0.745738 | 7.2:+35631731 | None:intergenic |
| GACGAGCAGAAAATTCCCTG+AGG | 0.767412 | 7.2:+35622400 | None:intergenic |
| GTCCAAGGGAAAGAACACTG+AGG | 0.796885 | 7.2:-35629915 | MS.gene77325:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 35621951 | 35631797 | 35621951 | ID=MS.gene77325 |
| chr7.2 | mRNA | 35621951 | 35631797 | 35621951 | ID=MS.gene77325.t1;Parent=MS.gene77325 |
| chr7.2 | exon | 35631743 | 35631797 | 35631743 | ID=MS.gene77325.t1.exon1;Parent=MS.gene77325.t1 |
| chr7.2 | CDS | 35631743 | 35631797 | 35631743 | ID=cds.MS.gene77325.t1;Parent=MS.gene77325.t1 |
| chr7.2 | exon | 35630543 | 35630812 | 35630543 | ID=MS.gene77325.t1.exon2;Parent=MS.gene77325.t1 |
| chr7.2 | CDS | 35630543 | 35630812 | 35630543 | ID=cds.MS.gene77325.t1;Parent=MS.gene77325.t1 |
| chr7.2 | exon | 35630070 | 35630357 | 35630070 | ID=MS.gene77325.t1.exon3;Parent=MS.gene77325.t1 |
| chr7.2 | CDS | 35630070 | 35630357 | 35630070 | ID=cds.MS.gene77325.t1;Parent=MS.gene77325.t1 |
| chr7.2 | exon | 35629915 | 35629962 | 35629915 | ID=MS.gene77325.t1.exon4;Parent=MS.gene77325.t1 |
| chr7.2 | CDS | 35629915 | 35629962 | 35629915 | ID=cds.MS.gene77325.t1;Parent=MS.gene77325.t1 |
| chr7.2 | exon | 35628655 | 35628869 | 35628655 | ID=MS.gene77325.t1.exon5;Parent=MS.gene77325.t1 |
| chr7.2 | CDS | 35628655 | 35628869 | 35628655 | ID=cds.MS.gene77325.t1;Parent=MS.gene77325.t1 |
| chr7.2 | exon | 35626951 | 35627124 | 35626951 | ID=MS.gene77325.t1.exon6;Parent=MS.gene77325.t1 |
| chr7.2 | CDS | 35626951 | 35627124 | 35626951 | ID=cds.MS.gene77325.t1;Parent=MS.gene77325.t1 |
| chr7.2 | exon | 35623754 | 35623877 | 35623754 | ID=MS.gene77325.t1.exon7;Parent=MS.gene77325.t1 |
| chr7.2 | CDS | 35623754 | 35623877 | 35623754 | ID=cds.MS.gene77325.t1;Parent=MS.gene77325.t1 |
| chr7.2 | exon | 35621951 | 35622492 | 35621951 | ID=MS.gene77325.t1.exon8;Parent=MS.gene77325.t1 |
| chr7.2 | CDS | 35621951 | 35622492 | 35621951 | ID=cds.MS.gene77325.t1;Parent=MS.gene77325.t1 |
| Gene Sequence |
| Protein sequence |
