Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78629.t1 | XP_003590374.1 | 93.8 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 2.90E-53 | 217.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78629.t1 | G7I999 | 93.8 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 2.1e-53 | 217.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049230 | MS.gene78629 | -0.841393 | 4.88E-58 | -1.69E-46 |
| MS.gene049378 | MS.gene78629 | -0.879055 | 1.70E-69 | -1.69E-46 |
| MS.gene04991 | MS.gene78629 | 0.882728 | 8.16E-71 | -1.69E-46 |
| MS.gene050105 | MS.gene78629 | -0.813015 | 3.17E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene78629 | -0.800248 | 1.58E-48 | -1.69E-46 |
| MS.gene050227 | MS.gene78629 | -0.814105 | 1.82E-51 | -1.69E-46 |
| MS.gene050228 | MS.gene78629 | -0.814389 | 1.58E-51 | -1.69E-46 |
| MS.gene050623 | MS.gene78629 | 0.861863 | 7.61E-64 | -1.69E-46 |
| MS.gene051064 | MS.gene78629 | -0.826309 | 2.91E-54 | -1.69E-46 |
| MS.gene051122 | MS.gene78629 | -0.845171 | 4.80E-59 | -1.69E-46 |
| MS.gene051983 | MS.gene78629 | -0.818817 | 1.61E-52 | -1.69E-46 |
| MS.gene052176 | MS.gene78629 | -0.802636 | 5.11E-49 | -1.69E-46 |
| MS.gene052389 | MS.gene78629 | -0.834898 | 2.30E-56 | -1.69E-46 |
| MS.gene052420 | MS.gene78629 | -0.825925 | 3.59E-54 | -1.69E-46 |
| MS.gene052464 | MS.gene78629 | -0.80827 | 3.36E-50 | -1.69E-46 |
| MS.gene052586 | MS.gene78629 | -0.812593 | 3.92E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene78629 | -0.84179 | 3.84E-58 | -1.69E-46 |
| MS.gene054930 | MS.gene78629 | -0.806375 | 8.48E-50 | -1.69E-46 |
| MS.gene055225 | MS.gene78629 | -0.802902 | 4.50E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene78629 | -0.852618 | 4.10E-61 | -1.69E-46 |
| MS.gene05595 | MS.gene78629 | -0.80254 | 5.35E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene78629 | -0.811479 | 6.86E-51 | -1.69E-46 |
| MS.gene057156 | MS.gene78629 | 0.81241 | 4.30E-51 | -1.69E-46 |
| MS.gene05761 | MS.gene78629 | -0.831529 | 1.59E-55 | -1.69E-46 |
| MS.gene058223 | MS.gene78629 | 0.844295 | 8.26E-59 | -1.69E-46 |
| MS.gene05843 | MS.gene78629 | 0.813442 | 2.55E-51 | -1.69E-46 |
| MS.gene060850 | MS.gene78629 | -0.813808 | 2.12E-51 | -1.69E-46 |
| MS.gene060851 | MS.gene78629 | -0.846561 | 2.01E-59 | -1.69E-46 |
| MS.gene060852 | MS.gene78629 | -0.845758 | 3.32E-59 | -1.69E-46 |
| MS.gene060879 | MS.gene78629 | -0.809545 | 1.79E-50 | -1.69E-46 |
| MS.gene060940 | MS.gene78629 | -0.822385 | 2.44E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78629.t1 | MTR_1g059290 | 93.750 | 112 | 7 | 0 | 1 | 112 | 1 | 112 | 4.90e-66 | 195 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78629.t1 | AT2G42310 | 68.932 | 103 | 29 | 1 | 2 | 104 | 14 | 113 | 7.69e-50 | 154 |
| MS.gene78629.t1 | AT3G57785 | 71.000 | 100 | 28 | 1 | 6 | 104 | 14 | 113 | 2.51e-49 | 152 |
Find 44 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCGTCTCCGCGGCGGTTC+AGG | 0.224776 | 1.4:-41796410 | MS.gene78629:CDS |
| TCAAATCAGGCTTAGCATTA+AGG | 0.309891 | 1.4:+41796225 | None:intergenic |
| AGCCCTTGAACGTCTCAAAT+TGG | 0.351342 | 1.4:-41796177 | MS.gene78629:CDS |
| CTGATTTGACTATTGAAACT+TGG | 0.354374 | 1.4:-41796211 | MS.gene78629:CDS |
| TAGGTCTTTCTTCGTGGCCT+GGG | 0.366840 | 1.4:+41796363 | None:intergenic |
| TTAGGTCTTTCTTCGTGGCC+TGG | 0.383907 | 1.4:+41796362 | None:intergenic |
| TGGTTGAAGAGGTAGCCTTT+AGG | 0.396876 | 1.4:+41796344 | None:intergenic |
| CAAGTTTCAATAGTCAAATC+AGG | 0.400055 | 1.4:+41796212 | None:intergenic |
| CTCCGCGGCGGTTCAGGAAC+CGG | 0.401455 | 1.4:-41796404 | MS.gene78629:CDS |
| CACCTGGAGCGAGTGGTGTT+TGG | 0.403944 | 1.4:+41796324 | None:intergenic |
| AATCACGCAAATGGGAAGAT+TGG | 0.415297 | 1.4:-41796301 | MS.gene78629:CDS |
| CTCCAGGTGAATCACGCAAA+TGG | 0.420212 | 1.4:-41796310 | MS.gene78629:CDS |
| CTTCCAATTTGAGACGTTCA+AGG | 0.438923 | 1.4:+41796174 | None:intergenic |
| TCCCATTTGCGTGATTCACC+TGG | 0.448242 | 1.4:+41796308 | None:intergenic |
| ATCACGCAAATGGGAAGATT+GGG | 0.466913 | 1.4:-41796300 | MS.gene78629:CDS |
| CGAGTGGTGTTTGGTTGAAG+AGG | 0.470232 | 1.4:+41796333 | None:intergenic |
| CGTGGCCTGGGCTTGTCCAT+CGG | 0.482648 | 1.4:+41796375 | None:intergenic |
| GCTTGTCCATCGGCTTGCAC+CGG | 0.486213 | 1.4:+41796385 | None:intergenic |
| TCCAGGTGAATCACGCAAAT+GGG | 0.489078 | 1.4:-41796309 | MS.gene78629:CDS |
| TGATTTGACTATTGAAACTT+GGG | 0.489933 | 1.4:-41796210 | MS.gene78629:CDS |
| AACCAAACACCACTCGCTCC+AGG | 0.505049 | 1.4:-41796326 | MS.gene78629:CDS |
| AGCCTTTAGGTCTTTCTTCG+TGG | 0.508189 | 1.4:+41796357 | None:intergenic |
| GCAAGCCGATGGACAAGCCC+AGG | 0.525769 | 1.4:-41796380 | MS.gene78629:CDS |
| GAGGAGACGTTGGCGGAGCA+TGG | 0.529777 | 1.4:+41796439 | None:intergenic |
| TTCCTGAACCGCCGCGGAGA+CGG | 0.531358 | 1.4:+41796408 | None:intergenic |
| TTCCTCACCATCGTTATCCT+CGG | 0.531600 | 1.4:-41796254 | MS.gene78629:CDS |
| TCAAAATCATCTCTATTCAT+CGG | 0.537178 | 1.4:+41796109 | None:intergenic |
| ACCATCGTTATCCTCGGTGT+TGG | 0.550397 | 1.4:-41796248 | MS.gene78629:CDS |
| CAGGAACCGGTGCAAGCCGA+TGG | 0.563436 | 1.4:-41796391 | MS.gene78629:CDS |
| TTCCAATTTGAGACGTTCAA+GGG | 0.567951 | 1.4:+41796175 | None:intergenic |
| GGAGAGAGGTGAGGAGACGT+TGG | 0.578698 | 1.4:+41796429 | None:intergenic |
| GAGAGGTGAGGAGACGTTGG+CGG | 0.579804 | 1.4:+41796432 | None:intergenic |
| GGCCACGAAGAAAGACCTAA+AGG | 0.583150 | 1.4:-41796359 | MS.gene78629:CDS |
| CGCGGAGACGGAGAGAGGTG+AGG | 0.590890 | 1.4:+41796420 | None:intergenic |
| ACCTCTCTCCGTCTCCGCGG+CGG | 0.593678 | 1.4:-41796416 | MS.gene78629:CDS |
| ACCGCCGCGGAGACGGAGAG+AGG | 0.601010 | 1.4:+41796415 | None:intergenic |
| CGTGATTCACCTGGAGCGAG+TGG | 0.616656 | 1.4:+41796317 | None:intergenic |
| AGGAAGCTAGTGATGTAGCA+AGG | 0.627360 | 1.4:+41796272 | None:intergenic |
| CACCGAGGATAACGATGGTG+AGG | 0.629292 | 1.4:+41796252 | None:intergenic |
| CACCGGTTCCTGAACCGCCG+CGG | 0.646325 | 1.4:+41796402 | None:intergenic |
| GGAATCATCAACGACGACAG+CGG | 0.672278 | 1.4:+41796130 | None:intergenic |
| GCCAACACCGAGGATAACGA+TGG | 0.718051 | 1.4:+41796247 | None:intergenic |
| TAGCATTAAGGCCAACACCG+AGG | 0.728768 | 1.4:+41796237 | None:intergenic |
| CTCACCTCTCTCCGTCTCCG+CGG | 0.734494 | 1.4:-41796419 | MS.gene78629:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATTTGACTATTGAAACTT+GGG | - | chr1.4:41796348-41796367 | MS.gene78629:CDS | 25.0% |
| CAAGTTTCAATAGTCAAATC+AGG | + | chr1.4:41796349-41796368 | None:intergenic | 30.0% | |
| ! | CTGATTTGACTATTGAAACT+TGG | - | chr1.4:41796347-41796366 | MS.gene78629:CDS | 30.0% |
| TCAAATCAGGCTTAGCATTA+AGG | + | chr1.4:41796336-41796355 | None:intergenic | 35.0% | |
| ! | TTCCAATTTGAGACGTTCAA+GGG | + | chr1.4:41796386-41796405 | None:intergenic | 35.0% |
| AATCACGCAAATGGGAAGAT+TGG | - | chr1.4:41796257-41796276 | MS.gene78629:CDS | 40.0% | |
| ATCACGCAAATGGGAAGATT+GGG | - | chr1.4:41796258-41796277 | MS.gene78629:CDS | 40.0% | |
| ! | CTTCCAATTTGAGACGTTCA+AGG | + | chr1.4:41796387-41796406 | None:intergenic | 40.0% |
| AGCCCTTGAACGTCTCAAAT+TGG | - | chr1.4:41796381-41796400 | MS.gene78629:CDS | 45.0% | |
| AGCCTTTAGGTCTTTCTTCG+TGG | + | chr1.4:41796204-41796223 | None:intergenic | 45.0% | |
| AGGAAGCTAGTGATGTAGCA+AGG | + | chr1.4:41796289-41796308 | None:intergenic | 45.0% | |
| TCCAGGTGAATCACGCAAAT+GGG | - | chr1.4:41796249-41796268 | MS.gene78629:CDS | 45.0% | |
| TGGTTGAAGAGGTAGCCTTT+AGG | + | chr1.4:41796217-41796236 | None:intergenic | 45.0% | |
| TTCCTCACCATCGTTATCCT+CGG | - | chr1.4:41796304-41796323 | MS.gene78629:CDS | 45.0% | |
| CGAGTGGTGTTTGGTTGAAG+AGG | + | chr1.4:41796228-41796247 | None:intergenic | 50.0% | |
| CTCCAGGTGAATCACGCAAA+TGG | - | chr1.4:41796248-41796267 | MS.gene78629:CDS | 50.0% | |
| GGAATCATCAACGACGACAG+CGG | + | chr1.4:41796431-41796450 | None:intergenic | 50.0% | |
| GGCCACGAAGAAAGACCTAA+AGG | - | chr1.4:41796199-41796218 | MS.gene78629:CDS | 50.0% | |
| TAGCATTAAGGCCAACACCG+AGG | + | chr1.4:41796324-41796343 | None:intergenic | 50.0% | |
| TAGGTCTTTCTTCGTGGCCT+GGG | + | chr1.4:41796198-41796217 | None:intergenic | 50.0% | |
| TCCCATTTGCGTGATTCACC+TGG | + | chr1.4:41796253-41796272 | None:intergenic | 50.0% | |
| TTAGGTCTTTCTTCGTGGCC+TGG | + | chr1.4:41796199-41796218 | None:intergenic | 50.0% | |
| ! | ACCATCGTTATCCTCGGTGT+TGG | - | chr1.4:41796310-41796329 | MS.gene78629:CDS | 50.0% |
| AACCAAACACCACTCGCTCC+AGG | - | chr1.4:41796232-41796251 | MS.gene78629:CDS | 55.0% | |
| CACCGAGGATAACGATGGTG+AGG | + | chr1.4:41796309-41796328 | None:intergenic | 55.0% | |
| GCCAACACCGAGGATAACGA+TGG | + | chr1.4:41796314-41796333 | None:intergenic | 55.0% | |
| CACCTGGAGCGAGTGGTGTT+TGG | + | chr1.4:41796237-41796256 | None:intergenic | 60.0% | |
| CGTGATTCACCTGGAGCGAG+TGG | + | chr1.4:41796244-41796263 | None:intergenic | 60.0% | |
| GAGAGGTGAGGAGACGTTGG+CGG | + | chr1.4:41796129-41796148 | None:intergenic | 60.0% | |
| GGAGAGAGGTGAGGAGACGT+TGG | + | chr1.4:41796132-41796151 | None:intergenic | 60.0% | |
| !! | GCTTGTCCATCGGCTTGCAC+CGG | + | chr1.4:41796176-41796195 | None:intergenic | 60.0% |
| CAGGAACCGGTGCAAGCCGA+TGG | - | chr1.4:41796167-41796186 | MS.gene78629:CDS | 65.0% | |
| CGTGGCCTGGGCTTGTCCAT+CGG | + | chr1.4:41796186-41796205 | None:intergenic | 65.0% | |
| CTCACCTCTCTCCGTCTCCG+CGG | - | chr1.4:41796139-41796158 | MS.gene78629:CDS | 65.0% | |
| GCAAGCCGATGGACAAGCCC+AGG | - | chr1.4:41796178-41796197 | MS.gene78629:CDS | 65.0% | |
| TTCCTGAACCGCCGCGGAGA+CGG | + | chr1.4:41796153-41796172 | None:intergenic | 65.0% | |
| ACCTCTCTCCGTCTCCGCGG+CGG | - | chr1.4:41796142-41796161 | MS.gene78629:CDS | 70.0% | |
| CGCGGAGACGGAGAGAGGTG+AGG | + | chr1.4:41796141-41796160 | None:intergenic | 70.0% | |
| CTCCGCGGCGGTTCAGGAAC+CGG | - | chr1.4:41796154-41796173 | MS.gene78629:CDS | 70.0% | |
| !! | CACCGGTTCCTGAACCGCCG+CGG | + | chr1.4:41796159-41796178 | None:intergenic | 70.0% |
| ACCGCCGCGGAGACGGAGAG+AGG | + | chr1.4:41796146-41796165 | None:intergenic | 75.0% | |
| CTCCGTCTCCGCGGCGGTTC+AGG | - | chr1.4:41796148-41796167 | MS.gene78629:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 41796121 | 41796459 | 41796121 | ID=MS.gene78629 |
| chr1.4 | mRNA | 41796121 | 41796459 | 41796121 | ID=MS.gene78629.t1;Parent=MS.gene78629 |
| chr1.4 | exon | 41796121 | 41796459 | 41796121 | ID=MS.gene78629.t1.exon1;Parent=MS.gene78629.t1 |
| chr1.4 | CDS | 41796121 | 41796459 | 41796121 | ID=cds.MS.gene78629.t1;Parent=MS.gene78629.t1 |
| Gene Sequence |
| Protein sequence |
