AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81300.t1 MTR_5g096150 97.834 277 6 0 1 277 43 319 0.0 567
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81300.t1 AT4G37000 53.214 280 126 2 1 277 41 318 2.32e-101 299

Find 50 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTACGACCTTGCTTCTTC+TGG 0.137652 5.2:+80725986 None:intergenic
ATCAGCTCGAACACGAAATT+TGG 0.213505 5.2:+80724715 None:intergenic
CTCGAAAAGACCTTGTTCTT+TGG 0.238806 5.2:-80724648 MS.gene81300:CDS
CAAAGCAAGTGTTGGGGATA+TGG 0.247429 5.2:-80724414 MS.gene81300:CDS
CCCAACACTTGCTTTGAAAT+CGG 0.292400 5.2:+80724421 None:intergenic
AGAACAAGAATTAGCATTGT+TGG 0.348949 5.2:+80724682 None:intergenic
TCAAGCTTTCCAAGGTTGTT+TGG 0.358482 5.2:-80724283 MS.gene81300:CDS
AACAGTAAAATACTCCTTGA+TGG 0.363131 5.2:+80724231 None:intergenic
GCTACGACCTTGCTTCTTCT+GGG 0.370091 5.2:+80725987 None:intergenic
AAGACTATTGAGGTTGATCT+TGG 0.379733 5.2:-80724307 MS.gene81300:CDS
AGAAGCTGCAAAACAGATAT+TGG 0.387915 5.2:-80724257 MS.gene81300:CDS
CTCTTATAGTATTGAACATC+AGG 0.390571 5.2:+80725853 None:intergenic
TCCTACTTCTCTCTCGGAAC+AGG 0.405515 5.2:+80724378 None:intergenic
CCGATTTCAAAGCAAGTGTT+GGG 0.413297 5.2:-80724421 MS.gene81300:CDS
CCAACACTTGCTTTGAAATC+GGG 0.426823 5.2:+80724422 None:intergenic
TTAGCATTGTTGGACTGCTT+CGG 0.427662 5.2:+80724692 None:intergenic
CCCGATTTCAAAGCAAGTGT+TGG 0.432798 5.2:-80724422 MS.gene81300:CDS
CGATACACATAGGCAGGCTT+TGG 0.432959 5.2:-80724587 MS.gene81300:CDS
GTGACATAAGGTTGAACCTC+TGG 0.461727 5.2:+80724556 None:intergenic
ATCATTTGAACTGTTAAGAT+AGG 0.468826 5.2:+80724741 None:intergenic
CAATACTATAAGAGCAAGAA+TGG 0.480003 5.2:-80725844 MS.gene81300:CDS
ATCAAGCATGAGATTCTTGT+GGG 0.484852 5.2:+80725933 None:intergenic
TTCCTCTCCTACTTCTCTCT+CGG 0.487400 5.2:+80724372 None:intergenic
ACAGATATTGGAAGCCATCA+AGG 0.487475 5.2:-80724245 MS.gene81300:CDS
ATCTTGGTTCAAGCTTTCCA+AGG 0.509846 5.2:-80724291 MS.gene81300:CDS
GTGCATGCTGAGCTACCAAC+AGG 0.516588 5.2:-80724793 MS.gene81300:CDS
TTGTGGGGTGCTGAAACAAA+TGG 0.519655 5.2:+80725949 None:intergenic
ATGAAGGAGAGCGAATAGAC+AGG 0.532992 5.2:-80724465 MS.gene81300:CDS
CATTAGAAACAAGACTATTG+AGG 0.540436 5.2:-80724317 MS.gene81300:CDS
ACAGGGGAATCAGTGTTGCT+TGG 0.543335 5.2:+80725793 None:intergenic
TAAACTCGATACACATAGGC+AGG 0.546034 5.2:-80724593 MS.gene81300:CDS
ATTCAGACAGAAAATGATGA+AGG 0.546561 5.2:-80724481 MS.gene81300:CDS
AAAAGCGAAGAAGTGACATA+AGG 0.548015 5.2:+80724544 None:intergenic
GCTTCTGGACCAAACAACCT+TGG 0.551283 5.2:+80724274 None:intergenic
GCAAGTGTTGGGGATATGGT+TGG 0.554687 5.2:-80724410 MS.gene81300:CDS
ATGCGAACAAATATAGCGGT+TGG 0.559048 5.2:+80724502 None:intergenic
ACATCAGGTGGAAGTGAACA+AGG 0.566315 5.2:+80725868 None:intergenic
ATATCTAATGATCCTCCTGT+TGG 0.577783 5.2:+80724778 None:intergenic
GATCAAGCATGAGATTCTTG+TGG 0.577920 5.2:+80725932 None:intergenic
GCCTGTTCCGAGAGAGAAGT+AGG 0.581979 5.2:-80724379 MS.gene81300:CDS
GGTAATCTGGCCAAAGAACA+AGG 0.591389 5.2:+80724638 None:intergenic
TTCCGAGAGAGAAGTAGGAG+AGG 0.601529 5.2:-80724374 MS.gene81300:CDS
TTATAGTATTGAACATCAGG+TGG 0.606078 5.2:+80725856 None:intergenic
ATGGATACCCAGAAGAAGCA+AGG 0.631772 5.2:-80725994 MS.gene81300:CDS
CGATTTCAAAGCAAGTGTTG+GGG 0.632172 5.2:-80724420 MS.gene81300:CDS
ACACTAAACTCGATACACAT+AGG 0.635620 5.2:-80724597 MS.gene81300:CDS
CATGCTGAGCTACCAACAGG+AGG 0.671949 5.2:-80724790 MS.gene81300:CDS
CTGAATGCGAACAAATATAG+CGG 0.692980 5.2:+80724498 None:intergenic
TCAAGCATGAGATTCTTGTG+GGG 0.732179 5.2:+80725934 None:intergenic
CGGTTGGAGATGCAACACTG+CGG 0.782235 5.2:+80724518 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTGTTATGTTATTTTATT+AGG - chr5.2:80724534-80724553 MS.gene81300:CDS 10.0%
!! ATTATGTTTCTTCAACATAA+GGG + chr5.2:80725057-80725076 None:intergenic 20.0%
!! TATACTCTAACATGAAAATA+TGG + chr5.2:80724486-80724505 None:intergenic 20.0%
!! TCATATAACAAAACAATCAA+TGG + chr5.2:80725270-80725289 None:intergenic 20.0%
!! TTCATGTTAGAGTATATATT+TGG - chr5.2:80724490-80724509 MS.gene81300:CDS 20.0%
!! TTTAAAAGCAAAAAGTAGAA+AGG + chr5.2:80724460-80724479 None:intergenic 20.0%
!!! TAAAAAGTTTTAAGGTAATC+TGG + chr5.2:80725600-80725619 None:intergenic 20.0%
! AAAAGTAGAAAGGAAATTAC+AGG + chr5.2:80724450-80724469 None:intergenic 25.0%
! AAAGTAGAAAGGAAATTACA+GGG + chr5.2:80724449-80724468 None:intergenic 25.0%
! ATCATTTGAACTGTTAAGAT+AGG + chr5.2:80725484-80725503 None:intergenic 25.0%
! ATGCAAATAACCAAATTAAG+TGG + chr5.2:80724706-80724725 None:intergenic 25.0%
! CATTATGTTTCTTCAACATA+AGG + chr5.2:80725058-80725077 None:intergenic 25.0%
! CTATCTAACTATCTACAAAT+AGG - chr5.2:80724981-80725000 MS.gene81300:intron 25.0%
! TATATATTTGGTTGAGAACT+AGG - chr5.2:80724502-80724521 MS.gene81300:CDS 25.0%
! TTCCTTAATTGTGATTGTTT+GGG - chr5.2:80724898-80724917 MS.gene81300:intron 25.0%
! TTTAGAGAACTGAATCAAAT+TGG - chr5.2:80724790-80724809 MS.gene81300:CDS 25.0%
!! TCTCAGATAGATTTTGTAAT+GGG - chr5.2:80725399-80725418 MS.gene81300:intron 25.0%
!! TTACCTTAAAACTTTTTACG+AGG - chr5.2:80725602-80725621 MS.gene81300:intron 25.0%
!!! TATTTTTCCATTAGTTCTCT+AGG - chr5.2:80725197-80725216 MS.gene81300:intron 25.0%
AACAGTAAAATACTCCTTGA+TGG + chr5.2:80725994-80726013 None:intergenic 30.0%
AAGTAGAAAGGAAATTACAG+GGG + chr5.2:80724448-80724467 None:intergenic 30.0%
AGAACAAGAATTAGCATTGT+TGG + chr5.2:80725543-80725562 None:intergenic 30.0%
ATTCAGACAGAAAATGATGA+AGG - chr5.2:80725741-80725760 MS.gene81300:intron 30.0%
CAATACTATAAGAGCAAGAA+TGG - chr5.2:80724378-80724397 MS.gene81300:CDS 30.0%
CATTAGAAACAAGACTATTG+AGG - chr5.2:80725905-80725924 MS.gene81300:CDS 30.0%
GAAGAAACATAATGTTCATG+AGG - chr5.2:80725064-80725083 MS.gene81300:intron 30.0%
GCTTATCTGATAAAAAGAGA+TGG - chr5.2:80725879-80725898 MS.gene81300:CDS 30.0%
GTGATTTCAACCACTTAATT+TGG - chr5.2:80724693-80724712 MS.gene81300:CDS 30.0%
GTTCCTTAATTGTGATTGTT+TGG - chr5.2:80724897-80724916 MS.gene81300:intron 30.0%
TACCCAAACAATCACAATTA+AGG + chr5.2:80724903-80724922 None:intergenic 30.0%
TCAAATAAGCTAATCCAAAC+AGG + chr5.2:80725090-80725109 None:intergenic 30.0%
! CTCTTATAGTATTGAACATC+AGG + chr5.2:80724372-80724391 None:intergenic 30.0%
! GTCTCAGATAGATTTTGTAA+TGG - chr5.2:80725398-80725417 MS.gene81300:intron 30.0%
! TGTCCTCGTAAAAAGTTTTA+AGG + chr5.2:80725608-80725627 None:intergenic 30.0%
! TTATAGTATTGAACATCAGG+TGG + chr5.2:80724369-80724388 None:intergenic 30.0%
AAAAGCGAAGAAGTGACATA+AGG + chr5.2:80725681-80725700 None:intergenic 35.0%
AAGCTATCCTAGAGAACTAA+TGG + chr5.2:80725207-80725226 None:intergenic 35.0%
ACACTAAACTCGATACACAT+AGG - chr5.2:80725625-80725644 MS.gene81300:intron 35.0%
AGAAGCTGCAAAACAGATAT+TGG - chr5.2:80725965-80725984 MS.gene81300:CDS 35.0%
ATATCTAATGATCCTCCTGT+TGG + chr5.2:80725447-80725466 None:intergenic 35.0%
ATCAAGCATGAGATTCTTGT+GGG + chr5.2:80724292-80724311 None:intergenic 35.0%
CAAGTTGTTTATCCAACACA+TGG - chr5.2:80725317-80725336 MS.gene81300:intron 35.0%
CATGTGTTGGATAAACAACT+TGG + chr5.2:80725319-80725338 None:intergenic 35.0%
CTGAATGCGAACAAATATAG+CGG + chr5.2:80725727-80725746 None:intergenic 35.0%
GCTTATTTGAGCTTACATAC+TGG - chr5.2:80725100-80725119 MS.gene81300:intron 35.0%
TTACTAACTACTCACCTTAG+AGG + chr5.2:80724653-80724672 None:intergenic 35.0%
! AAGACTATTGAGGTTGATCT+TGG - chr5.2:80725915-80725934 MS.gene81300:CDS 35.0%
! AATATCTGTTTTGCAGCTTC+TGG + chr5.2:80725966-80725985 None:intergenic 35.0%
! TAGAGTGTCTAAAGTGAGTT+TGG + chr5.2:80724616-80724635 None:intergenic 35.0%
!! AGATTTTGTAATGGGAAGCT+GGG - chr5.2:80725407-80725426 MS.gene81300:intron 35.0%
!! TAGATTTTGTAATGGGAAGC+TGG - chr5.2:80725406-80725425 MS.gene81300:intron 35.0%
ACAGATATTGGAAGCCATCA+AGG - chr5.2:80725977-80725996 MS.gene81300:CDS 40.0%
ACCATAACTAGACCATGTGT+TGG + chr5.2:80725332-80725351 None:intergenic 40.0%
ATCAGCTCGAACACGAAATT+TGG + chr5.2:80725510-80725529 None:intergenic 40.0%
ATCTACCAACCAGTGAATCA+AGG + chr5.2:80724871-80724890 None:intergenic 40.0%
ATCTTGGTTCAAGCTTTCCA+AGG - chr5.2:80725931-80725950 MS.gene81300:CDS 40.0%
ATGCGAACAAATATAGCGGT+TGG + chr5.2:80725723-80725742 None:intergenic 40.0%
CCGATTTCAAAGCAAGTGTT+GGG - chr5.2:80725801-80725820 MS.gene81300:CDS 40.0%
CTCGAAAAGACCTTGTTCTT+TGG - chr5.2:80725574-80725593 MS.gene81300:intron 40.0%
GATCAAGCATGAGATTCTTG+TGG + chr5.2:80724293-80724312 None:intergenic 40.0%
TAAACTCGATACACATAGGC+AGG - chr5.2:80725629-80725648 MS.gene81300:intron 40.0%
TAATGTTCATGAGGCCTGTT+TGG - chr5.2:80725073-80725092 MS.gene81300:intron 40.0%
TCAACCATGTTTCTCCTCTA+AGG - chr5.2:80724636-80724655 MS.gene81300:CDS 40.0%
TCAAGCATGAGATTCTTGTG+GGG + chr5.2:80724291-80724310 None:intergenic 40.0%
TCAAGCTTTCCAAGGTTGTT+TGG - chr5.2:80725939-80725958 MS.gene81300:CDS 40.0%
TCCAACACATGGTCTAGTTA+TGG - chr5.2:80725328-80725347 MS.gene81300:intron 40.0%
TTAGCATTGTTGGACTGCTT+CGG + chr5.2:80725533-80725552 None:intergenic 40.0%
! CAAAGAACAAGGTCTTTTCG+AGG + chr5.2:80725576-80725595 None:intergenic 40.0%
! CGATTTCAAAGCAAGTGTTG+GGG - chr5.2:80725802-80725821 MS.gene81300:CDS 40.0%
! GCAAGGTCGTAGCAAATTTT+TGG - chr5.2:80724245-80724264 MS.gene81300:CDS 40.0%
!! CCAACACTTGCTTTGAAATC+GGG + chr5.2:80725803-80725822 None:intergenic 40.0%
!! CCCAACACTTGCTTTGAAAT+CGG + chr5.2:80725804-80725823 None:intergenic 40.0%
!! GGCTTTGGAAAAAATTCCAG+AGG - chr5.2:80725650-80725669 MS.gene81300:intron 40.0%
ACATCAGGTGGAAGTGAACA+AGG + chr5.2:80724357-80724376 None:intergenic 45.0%
ATGAAGGAGAGCGAATAGAC+AGG - chr5.2:80725757-80725776 MS.gene81300:intron 45.0%
ATGGATACCCAGAAGAAGCA+AGG - chr5.2:80724228-80724247 MS.gene81300:CDS 45.0%
CAACCAGTGAATCAAGGCTT+AGG + chr5.2:80724865-80724884 None:intergenic 45.0%
CCCGATTTCAAAGCAAGTGT+TGG - chr5.2:80725800-80725819 MS.gene81300:CDS 45.0%
CTAAGCCTTGATTCACTGGT+TGG - chr5.2:80724863-80724882 MS.gene81300:intron 45.0%
CTCACCTTAGAGGAGAAACA+TGG + chr5.2:80724643-80724662 None:intergenic 45.0%
GGTAATCTGGCCAAAGAACA+AGG + chr5.2:80725587-80725606 None:intergenic 45.0%
GTGACATAAGGTTGAACCTC+TGG + chr5.2:80725669-80725688 None:intergenic 45.0%
TAGCCTAAGCCTTGATTCAC+TGG - chr5.2:80724859-80724878 MS.gene81300:intron 45.0%
TTCCTCTCCTACTTCTCTCT+CGG + chr5.2:80725853-80725872 None:intergenic 45.0%
!! AGAACAAGGTCTTTTCGAGG+TGG + chr5.2:80725573-80725592 None:intergenic 45.0%
!! CAAAGCAAGTGTTGGGGATA+TGG - chr5.2:80725808-80725827 MS.gene81300:CDS 45.0%
!! TTGTGGGGTGCTGAAACAAA+TGG + chr5.2:80724276-80724295 None:intergenic 45.0%
!!! ATTATTTTAATTACTTATTA+AGG - chr5.2:80724943-80724962 MS.gene81300:intron 5.0%
CGATACACATAGGCAGGCTT+TGG - chr5.2:80725635-80725654 MS.gene81300:intron 50.0%
GCTACGACCTTGCTTCTTCT+GGG + chr5.2:80724238-80724257 None:intergenic 50.0%
GCTTCTGGACCAAACAACCT+TGG + chr5.2:80725951-80725970 None:intergenic 50.0%
TCCTACTTCTCTCTCGGAAC+AGG + chr5.2:80725847-80725866 None:intergenic 50.0%
TGCTACGACCTTGCTTCTTC+TGG + chr5.2:80724239-80724258 None:intergenic 50.0%
TTCCGAGAGAGAAGTAGGAG+AGG - chr5.2:80725848-80725867 MS.gene81300:CDS 50.0%
! ACAGGGGAATCAGTGTTGCT+TGG + chr5.2:80724432-80724451 None:intergenic 50.0%
!! GCAAGTGTTGGGGATATGGT+TGG - chr5.2:80725812-80725831 MS.gene81300:CDS 50.0%
CATGCTGAGCTACCAACAGG+AGG - chr5.2:80725432-80725451 MS.gene81300:intron 55.0%
CGGTTGGAGATGCAACACTG+CGG + chr5.2:80725707-80725726 None:intergenic 55.0%
GCCTGTTCCGAGAGAGAAGT+AGG - chr5.2:80725843-80725862 MS.gene81300:CDS 55.0%
GTGCATGCTGAGCTACCAAC+AGG - chr5.2:80725429-80725448 MS.gene81300:intron 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 80724228 80726016 80724228 ID=MS.gene81300
chr5.2 mRNA 80724228 80726016 80724228 ID=MS.gene81300.t1;Parent=MS.gene81300
chr5.2 exon 80725795 80726016 80725795 ID=MS.gene81300.t1.exon1;Parent=MS.gene81300.t1
chr5.2 CDS 80725795 80726016 80725795 ID=cds.MS.gene81300.t1;Parent=MS.gene81300.t1
chr5.2 exon 80724228 80724839 80724228 ID=MS.gene81300.t1.exon2;Parent=MS.gene81300.t1
chr5.2 CDS 80724228 80724839 80724228 ID=cds.MS.gene81300.t1;Parent=MS.gene81300.t1
Gene Sequence

>MS.gene81300

ATGGATACCCAGAAGAAGCAAGGTCGTAGCAAATTTTTGGAATTTCCATTTGTTTCAGCACCCCACAAGAATCTCATGCTTGATCTTGTTTCAACAGTTGAAGATCGCTTTGAATCTCATCTTCTTCCTTGTTCACTTCCACCTGATGTTCAATACTATAAGAGCAAGAATGGTACTTCTCAAGTTTCATTGCATATTAGACCAAGCAACACTGATTCCCCTGTAATTTCCTTTCTACTTTTTGCTTTTAAAATTCCATATTTTCATGTTAGAGTATATATTTGGTTGAGAACTAGGTACTTAATTTATTGTTATGTTATTTTATTAGGTTAAGTACACTAAATATTTATCATAGATTATGTTAATGTTTTTAATAAATATTTATCCAAACTCACTTTAGACACTCTATCAACCATGTTTCTCCTCTAAGGTGAGTAGTTAGTAAGATTAGAAAAATTAAGAATTGTGATTTCAACCACTTAATTTGGTTATTTGCATAATACATTAGTTTGTTGTTAATAGTTGATATTAGTAACTTTACTCTCTAAAATTAGTTGCTTACTTTAGAGAACTGAATCAAATTGGCAATGTAGATTTTTATTTTCATTTGAATAATTTTCATTTAATATTGTAGCCTAAGCCTTGATTCACTGGTTGGTAGATGTAATAGTTCCTTAATTGTGATTGTTTGGGTATTTTTTTGCATGTTGTTGAGATTATTTTAATTACTTATTAAGGAAATCATTAACTTATCTATCTAACTATCTACAAATAGGCACTGCAGATAGTTATTTTCTTCATAAATGATTTGTTATTTCAGTGAACTCCCTTATGTTGAAGAAACATAATGTTCATGAGGCCTGTTTGGATTAGCTTATTTGAGCTTACATACTGGCATAAGCACTTATGAGACTGTTTGATAGAACTTCTGAAAACAACTTATGACTTGTATATAGCTTGTTTTCAGCTTATTTTTCCATTAGTTCTCTAGGATAGCTTTATGAAAACAGCTCATAGCTTATATAAAAATATTTTGATTCCATTGATTGTTTTGTTATATGATAAGTGCTTATGTTATAAGCGTTTAACCAAGTTGTTTATCCAACACATGGTCTAGTTATGGTTTTATTCTGAATTTGTTGTTCACTGTTCATTTTGTTGTTGTTGTATGTCTCAGATAGATTTTGTAATGGGAAGCTGGGTGCATGCTGAGCTACCAACAGGAGGATCATTAGATATAACATCTCTTTCAGCCTATCTTAACAGTTCAAATGATGCACCAAATTTCGTGTTCGAGCTGATCCGAAGCAGTCCAACAATGCTAATTCTTGTTCTTGATTTGCCACCTCGAAAAGACCTTGTTCTTTGGCCAGATTACCTTAAAACTTTTTACGAGGACACTAAACTCGATACACATAGGCAGGCTTTGGAAAAAATTCCAGAGGTTCAACCTTATGTCACTTCTTCGCTTTTCATCCGCAGTGTTGCATCTCCAACCGCTATATTTGTTCGCATTCAGACAGAAAATGATGAAGGAGAGCGAATAGACAGGATCATCAAGAACAATATTGACCCGATTTCAAAGCAAGTGTTGGGGATATGGTTGGATCATTGCGCCTGTTCCGAGAGAGAAGTAGGAGAGGAAGATAAAGCTTATCTGATAAAAAGAGATGGTGTCATTAGAAACAAGACTATTGAGGTTGATCTTGGTTCAAGCTTTCCAAGGTTGTTTGGTCCAGAAGCTGCAAAACAGATATTGGAAGCCATCAAGGAGTATTTTACTGTTTGA

Protein sequence

>MS.gene81300.t1

MDTQKKQGRSKFLEFPFVSAPHKNLMLDLVSTVEDRFESHLLPCSLPPDVQYYKSKNGTSQVSLHIRPSNTDSPIDFVMGSWVHAELPTGGSLDITSLSAYLNSSNDAPNFVFELIRSSPTMLILVLDLPPRKDLVLWPDYLKTFYEDTKLDTHRQALEKIPEVQPYVTSSLFIRSVASPTAIFVRIQTENDEGERIDRIIKNNIDPISKQVLGIWLDHCACSEREVGEEDKAYLIKRDGVIRNKTIEVDLGSSFPRLFGPEAAKQILEAIKEYFTV