AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81831


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81831.t1 MTR_6g070920 91.824 159 1 1 1 147 72 230 4.45e-105 299
MS.gene81831.t1 MTR_6g070920 87.407 135 5 1 1 123 72 206 5.16e-83 243
MS.gene81831.t1 MTR_3g065330 55.696 158 53 4 1 142 10 166 3.17e-54 169
MS.gene81831.t1 MTR_1g032290 54.717 159 56 4 1 144 1 158 8.86e-54 167
MS.gene81831.t1 MTR_8g105140 54.037 161 58 4 1 146 1 160 2.06e-53 166
MS.gene81831.t1 MTR_5g078020 51.656 151 55 3 8 142 16 164 5.82e-46 148
MS.gene81831.t1 MTR_6g070920 81.319 91 5 1 1 79 72 162 1.46e-45 146
MS.gene81831.t1 MTR_3g065330 55.556 135 43 4 1 119 32 165 4.65e-43 140
MS.gene81831.t1 MTR_8g039690 41.509 159 72 3 2 139 1 159 1.78e-33 116
MS.gene81831.t1 MTR_7g084380 42.331 163 70 4 2 140 1 163 1.80e-32 114
MS.gene81831.t1 MTR_7g084380 40.288 139 59 4 26 140 21 159 2.40e-23 91.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81831.t1 AT1G18300 55.414 157 53 4 1 141 40 195 2.26e-55 172
MS.gene81831.t1 AT1G73540 55.414 157 53 4 1 143 41 194 3.93e-53 167
MS.gene81831.t1 AT1G14860 55.128 156 55 3 1 142 1 155 8.93e-53 165
MS.gene81831.t1 AT2G01670 50.932 161 64 3 1 147 6 165 3.50e-50 159
MS.gene81831.t1 AT1G12880 42.169 166 69 4 2 140 1 166 5.44e-33 115
MS.gene81831.t1 AT3G26690 40.120 167 72 4 2 140 1 167 8.81e-30 107
MS.gene81831.t1 AT3G26690 40.120 167 72 4 2 140 1 167 8.81e-30 107
MS.gene81831.t1 AT3G12600 43.750 160 67 4 2 138 1 160 1.86e-26 98.2
MS.gene81831.t1 AT3G12600 43.243 148 61 4 14 138 4 151 1.81e-22 87.8

Find 33 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTTCTTATGTTCATTTC+TGG 0.092026 6.3:+67489535 None:intergenic
TGCAGAGTAATTTGGGTAAA+TGG 0.215433 6.3:-67489639 MS.gene81831:intron
TGAACATAAGAAGAAGAAAA+TGG 0.253808 6.3:-67489528 MS.gene81831:CDS
ATAGAAGAAGCTGGAGTTAT+TGG 0.256499 6.3:-67489774 MS.gene81831:CDS
AATAACTCCAGCTTCTTCTA+TGG 0.282235 6.3:+67489776 None:intergenic
TTAGCAAGGAGTTGGATAAT+TGG 0.322855 6.3:-67489558 MS.gene81831:CDS
GAAGGAAGCTTTAGATGAAT+TGG 0.336584 6.3:-67489122 MS.gene81831:CDS
ACCTAAAACTTAGATTGAAT+TGG 0.341917 6.3:+67489087 None:intergenic
TGCAAATGTCTACCAGTTCT+AGG 0.360990 6.3:+67490517 None:intergenic
GCTTCCTTCATCCAAGCATA+TGG 0.390570 6.3:+67489136 None:intergenic
AAGAAATATGCCCATATGCT+TGG 0.435806 6.3:-67489147 MS.gene81831:CDS
TGTTTGGTTTCTCCTAGAAC+TGG 0.445974 6.3:-67490529 MS.gene81831:CDS
ATTGGAGAATATGATGTGTT+TGG 0.449206 6.3:-67490545 None:intergenic
GAACATAAGAAGAAGAAAAT+GGG 0.454169 6.3:-67489527 MS.gene81831:intron
GTTCCCTTTGCTTGTTAGCA+AGG 0.472162 6.3:-67489572 MS.gene81831:CDS
TTTGCTTGTTAGCAAGGAGT+TGG 0.481309 6.3:-67489566 MS.gene81831:CDS
AAAGAATGGAACTCAAGAAA+AGG 0.494824 6.3:-67489904 MS.gene81831:CDS
TCTCAAAGCAGCTTGTTCCA+TGG 0.496073 6.3:+67489803 None:intergenic
AGAGAAACCATAGAAGAAGC+TGG 0.505150 6.3:-67489783 MS.gene81831:CDS
AACTCAAGAAAAGGAAATAG+AGG 0.509278 6.3:-67489895 MS.gene81831:CDS
AACTCCTTGCTAACAAGCAA+AGG 0.516565 6.3:+67489568 None:intergenic
CTTCCTTCATCCAAGCATAT+GGG 0.522789 6.3:+67489137 None:intergenic
ACAAGGATGTCGTCAAGTTG+TGG 0.541451 6.3:-67490485 MS.gene81831:CDS
CATGTATCCTTCATGCATAG+TGG 0.542137 6.3:+67489594 None:intergenic
CAAGGATGTCGTCAAGTTGT+GGG 0.550727 6.3:-67490484 MS.gene81831:CDS
ACTCCTTGCTAACAAGCAAA+GGG 0.551729 6.3:+67489569 None:intergenic
ATGCCCATATGCTTGGATGA+AGG 0.557447 6.3:-67489140 MS.gene81831:CDS
ATCCTTCATGCATAGTGGGT+TGG 0.562217 6.3:+67489599 None:intergenic
ATGTATCCTTCATGCATAGT+GGG 0.569535 6.3:+67489595 None:intergenic
CATTTGCAACGTTATGAACA+AGG 0.571762 6.3:-67490502 MS.gene81831:CDS
CGCCAACCCACTATGCATGA+AGG 0.576057 6.3:-67489601 MS.gene81831:CDS
AAGGATGTCGTCAAGTTGTG+GGG 0.655005 6.3:-67490483 MS.gene81831:intron
TGTAATCATGCAGTTGACTG+TGG 0.660929 6.3:-67489179 MS.gene81831:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATAAAAGTTGAATAT+AGG + chr6.3:67490145-67490164 None:intergenic 10.0%
!!! ATTTTTCATGAAATAATATT+TGG - chr6.3:67489931-67489950 MS.gene81831:intron 10.0%
!! TTACAAATGAAAGAAATATT+AGG + chr6.3:67490437-67490456 None:intergenic 15.0%
!!! AAGTTTTGATTTTTAAGATT+TGG - chr6.3:67489462-67489481 MS.gene81831:intron 15.0%
!!! AATTTGAATTTGATTTATGA+GGG - chr6.3:67489635-67489654 MS.gene81831:CDS 15.0%
!!! GAAATCTTTTGAAATTTTTA+AGG - chr6.3:67489418-67489437 MS.gene81831:intron 15.0%
!!! GACAATTTATGAAATTTTTA+AGG - chr6.3:67489261-67489280 MS.gene81831:intron 15.0%
!!! GTTTTTTTTTTTTCTCAATT+GGG - chr6.3:67489351-67489370 MS.gene81831:intron 15.0%
!!! TAATTTGAATTTGATTTATG+AGG - chr6.3:67489634-67489653 MS.gene81831:CDS 15.0%
!!! TGTAATAACTTTTTTAGATT+TGG - chr6.3:67489902-67489921 MS.gene81831:CDS 15.0%
!! ATGATTCAACAATGAATAAA+AGG + chr6.3:67489567-67489586 None:intergenic 20.0%
!! TCTTAATTAAGTAGCATTAT+TGG - chr6.3:67490384-67490403 MS.gene81831:intron 20.0%
!! TGATTCAACAATGAATAAAA+GGG + chr6.3:67489566-67489585 None:intergenic 20.0%
!! TTTATAAATGACACTTGATT+AGG + chr6.3:67490318-67490337 None:intergenic 20.0%
!! TTTATTCATTGTTGAATCAT+TGG - chr6.3:67489567-67489586 MS.gene81831:CDS 20.0%
!!! AAAATTTTGAATTGAATACG+AGG - chr6.3:67489306-67489325 MS.gene81831:intron 20.0%
!!! AAATTTTGAATTGAATACGA+GGG - chr6.3:67489307-67489326 MS.gene81831:intron 20.0%
!!! AAGTTTGGATTTTTATTGTT+TGG - chr6.3:67489381-67489400 MS.gene81831:intron 20.0%
!!! ATATTGTGATTTGTTCTTAT+GGG - chr6.3:67489194-67489213 MS.gene81831:intron 20.0%
!!! ATTTTTAAGGTTTTTTCAGT+TGG - chr6.3:67489431-67489450 MS.gene81831:intron 20.0%
!!! GACAATTTTTGATAACTTTA+AGG - chr6.3:67489329-67489348 MS.gene81831:intron 20.0%
!!! GGTTTTTTTTTTTTCTCAAT+TGG - chr6.3:67489350-67489369 MS.gene81831:intron 20.0%
!!! TTTTAAGGTTTTTCTCAATT+GGG - chr6.3:67489276-67489295 MS.gene81831:intron 20.0%
!!! TTTTAATGTTTTTCTCAAGT+GGG - chr6.3:67489514-67489533 MS.gene81831:intron 20.0%
!!! TTTTTAAGGTTTTTCTCAAT+TGG - chr6.3:67489275-67489294 MS.gene81831:intron 20.0%
!!! TTTTTAAGGTTTTTTCAGTT+GGG - chr6.3:67489432-67489451 MS.gene81831:intron 20.0%
!!! TTTTTAATGTTTTTCTCAAG+TGG - chr6.3:67489513-67489532 MS.gene81831:intron 20.0%
! AGATGAATTGACATTTGAAT+AGG + chr6.3:67490350-67490369 None:intergenic 25.0%
! CCTTATAGATACAAAAAGAA+TGG - chr6.3:67489713-67489732 MS.gene81831:intron 25.0%
! CTTATGGGTTTCTTAAAAAT+TGG - chr6.3:67489209-67489228 MS.gene81831:intron 25.0%
! GAACATAAGAAGAAGAAAAT+GGG - chr6.3:67490104-67490123 MS.gene81831:intron 25.0%
! TGAACATAAGAAGAAGAAAA+TGG - chr6.3:67490103-67490122 MS.gene81831:intron 25.0%
!! CCATTCTTTTTGTATCTATA+AGG + chr6.3:67489716-67489735 None:intergenic 25.0%
!!! AAAAAAGTATTATGTCTGAG+CGG + chr6.3:67490246-67490265 None:intergenic 25.0%
!!! GATATTGTGATTTGTTCTTA+TGG - chr6.3:67489193-67489212 MS.gene81831:intron 25.0%
!!! GTTAATCTAACTTTGTTTTG+TGG - chr6.3:67489666-67489685 MS.gene81831:intron 25.0%
!!! TAACTTTGTTTTGTGGTTTT+TGG - chr6.3:67489673-67489692 MS.gene81831:intron 25.0%
!!! TTTAAGGTTTTTCTCAATTG+GGG - chr6.3:67489277-67489296 MS.gene81831:intron 25.0%
!!! TTTTAAGGTTTTTTCAGTTG+GGG - chr6.3:67489433-67489452 MS.gene81831:intron 25.0%
AAAGAATGGAACTCAAGAAA+AGG - chr6.3:67489727-67489746 MS.gene81831:intron 30.0%
AACTCAAGAAAAGGAAATAG+AGG - chr6.3:67489736-67489755 MS.gene81831:intron 30.0%
CAATTGGGTTTCTTAAAGTT+TGG - chr6.3:67489366-67489385 MS.gene81831:intron 30.0%
CTTCTTCTTATGTTCATTTC+TGG + chr6.3:67490099-67490118 None:intergenic 30.0%
TTCATTGTTGAATCATTGGA+AGG - chr6.3:67489571-67489590 MS.gene81831:CDS 30.0%
! CTTTTGATAAGTGCTCAAAA+GGG - chr6.3:67489761-67489780 MS.gene81831:intron 30.0%
! GTTTTCTTGCAGAGTAATTT+GGG - chr6.3:67489985-67490004 MS.gene81831:intron 30.0%
! TCTTTTGATAAGTGCTCAAA+AGG - chr6.3:67489760-67489779 MS.gene81831:intron 30.0%
! TGTTTTCTTGCAGAGTAATT+TGG - chr6.3:67489984-67490003 MS.gene81831:intron 30.0%
! TTGTTTGGAATTGTAGATGA+TGG - chr6.3:67489396-67489415 MS.gene81831:intron 30.0%
AAGAAATATGCCCATATGCT+TGG - chr6.3:67490484-67490503 MS.gene81831:CDS 35.0%
AATAACTCCAGCTTCTTCTA+TGG + chr6.3:67489858-67489877 None:intergenic 35.0%
ATAGAAGAAGCTGGAGTTAT+TGG - chr6.3:67489857-67489876 MS.gene81831:intron 35.0%
ATGTATCCTTCATGCATAGT+GGG + chr6.3:67490039-67490058 None:intergenic 35.0%
CATTTGCAACGTTATGAACA+AGG - chr6.3:67489129-67489148 MS.gene81831:CDS 35.0%
TGCAGAGTAATTTGGGTAAA+TGG - chr6.3:67489992-67490011 MS.gene81831:intron 35.0%
TTAGCAAGGAGTTGGATAAT+TGG - chr6.3:67490073-67490092 MS.gene81831:intron 35.0%
TTGGGAAAAAGATGAAACCA+TGG - chr6.3:67489811-67489830 MS.gene81831:CDS 35.0%
!! GAAGGAAGCTTTAGATGAAT+TGG - chr6.3:67490509-67490528 MS.gene81831:CDS 35.0%
AACTCCTTGCTAACAAGCAA+AGG + chr6.3:67490066-67490085 None:intergenic 40.0%
ACTCCTTGCTAACAAGCAAA+GGG + chr6.3:67490065-67490084 None:intergenic 40.0%
AGAGAAACCATAGAAGAAGC+TGG - chr6.3:67489848-67489867 MS.gene81831:intron 40.0%
AGTGGAATGCAGTTTCCAAA+AGG - chr6.3:67489785-67489804 MS.gene81831:CDS 40.0%
ATAAGTGCTCAAAAGGGTAG+TGG - chr6.3:67489767-67489786 MS.gene81831:CDS 40.0%
ATGACACTTGATTAGGCTCA+AGG + chr6.3:67490311-67490330 None:intergenic 40.0%
CATGTATCCTTCATGCATAG+TGG + chr6.3:67490040-67490059 None:intergenic 40.0%
CTTCCTTCATCCAAGCATAT+GGG + chr6.3:67490497-67490516 None:intergenic 40.0%
TGCAAATGTCTACCAGTTCT+AGG + chr6.3:67489117-67489136 None:intergenic 40.0%
TGTAATCATGCAGTTGACTG+TGG - chr6.3:67490452-67490471 MS.gene81831:intron 40.0%
! TCTTTTTCCCAACCTCCTTT+TGG + chr6.3:67489803-67489822 None:intergenic 40.0%
! TGTTTGGTTTCTCCTAGAAC+TGG - chr6.3:67489102-67489121 MS.gene81831:CDS 40.0%
! TTTGCTTGTTAGCAAGGAGT+TGG - chr6.3:67490065-67490084 MS.gene81831:intron 40.0%
ATGCCCATATGCTTGGATGA+AGG - chr6.3:67490491-67490510 MS.gene81831:CDS 45.0%
GCAGTTTCCAAAAGGAGGTT+GGG - chr6.3:67489793-67489812 MS.gene81831:CDS 45.0%
GCTTCCTTCATCCAAGCATA+TGG + chr6.3:67490498-67490517 None:intergenic 45.0%
GGAATGCAGTTTCCAAAAGG+AGG - chr6.3:67489788-67489807 MS.gene81831:CDS 45.0%
TGCAGTTTCCAAAAGGAGGT+TGG - chr6.3:67489792-67489811 MS.gene81831:CDS 45.0%
! AAGGATGTCGTCAAGTTGTG+GGG - chr6.3:67489148-67489167 MS.gene81831:CDS 45.0%
! ACAAGGATGTCGTCAAGTTG+TGG - chr6.3:67489146-67489165 MS.gene81831:CDS 45.0%
! CAAGGATGTCGTCAAGTTGT+GGG - chr6.3:67489147-67489166 MS.gene81831:CDS 45.0%
! GTTCCCTTTGCTTGTTAGCA+AGG - chr6.3:67490059-67490078 MS.gene81831:intron 45.0%
! TCTCAAAGCAGCTTGTTCCA+TGG + chr6.3:67489831-67489850 None:intergenic 45.0%
!! ATCCTTCATGCATAGTGGGT+TGG + chr6.3:67490035-67490054 None:intergenic 45.0%
TTATGTCGCAGCGCAGATTG+AGG - chr6.3:67490264-67490283 MS.gene81831:intron 50.0%
CGCCAACCCACTATGCATGA+AGG - chr6.3:67490030-67490049 MS.gene81831:intron 55.0%
TGTCTGAGCGGCTACGATTG+AGG + chr6.3:67490234-67490253 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 67489096 67490557 67489096 ID=MS.gene81831
chr6.3 mRNA 67489096 67490557 67489096 ID=MS.gene81831.t1;Parent=MS.gene81831
chr6.3 exon 67490484 67490557 67490484 ID=MS.gene81831.t1.exon1;Parent=MS.gene81831.t1
chr6.3 CDS 67490484 67490557 67490484 ID=cds.MS.gene81831.t1;Parent=MS.gene81831.t1
chr6.3 exon 67489868 67489947 67489868 ID=MS.gene81831.t1.exon2;Parent=MS.gene81831.t1
chr6.3 CDS 67489868 67489947 67489868 ID=cds.MS.gene81831.t1;Parent=MS.gene81831.t1
chr6.3 exon 67489764 67489831 67489764 ID=MS.gene81831.t1.exon3;Parent=MS.gene81831.t1
chr6.3 CDS 67489764 67489831 67489764 ID=cds.MS.gene81831.t1;Parent=MS.gene81831.t1
chr6.3 exon 67489528 67489656 67489528 ID=MS.gene81831.t1.exon4;Parent=MS.gene81831.t1
chr6.3 CDS 67489528 67489656 67489528 ID=cds.MS.gene81831.t1;Parent=MS.gene81831.t1
chr6.3 exon 67489096 67489188 67489096 ID=MS.gene81831.t1.exon5;Parent=MS.gene81831.t1
chr6.3 CDS 67489096 67489188 67489096 ID=cds.MS.gene81831.t1;Parent=MS.gene81831.t1
Gene Sequence

>MS.gene81831

ATGATGTGTTTGGTTTCTCCTAGAACTGGTAGACATTTGCAACGTTATGAACAAGGATGTCGTCAAGTTGTGGGGTGTGTTTCTGTTTCTCTCTCTTGATATTGTGATTTGTTCTTATGGGTTTCTTAAAAATTGGATTTTTAATGTTTAATATTGAATATAATTGACAATTTATGAAATTTTTAAGGTTTTTCTCAATTGGGGTTTCTTAAAATTTTGAATTGAATACGAGGGACAATTTTTGATAACTTTAAGGTTTTTTTTTTTTCTCAATTGGGTTTCTTAAAGTTTGGATTTTTATTGTTTGGAATTGTAGATGATGGAAATCTTTTGAAATTTTTAAGGTTTTTTCAGTTGGGGTTTTTGAAGTTTTGATTTTTAAGATTTGGAATTGAAGATGATGAACAATTTTTGAAATTTTTAATGTTTTTCTCAAGTGGGTTTTTTTCTAATGATTTTAAAGTATACCCTTTTATTCATTGTTGAATCATTGGAAGGAAAAGTGAAAAGTCAATTTTTTTTTTTTTTTTTGTGAATTTAATTTGAATTTGATTTATGAGGGTTTGAATTGTTAATCTAACTTTGTTTTGTGGTTTTTGGAATTGAACAGATGCATACCTTATAGATACAAAAAGAATGGAACTCAAGAAAAGGAAATAGAGGTTCTTTTGATAAGTGCTCAAAAGGGTAGTGGAATGCAGTTTCCAAAAGGAGGTTGGGAAAAAGATGAAACCATGGAACAAGCTGCTTTGAGAGAAACCATAGAAGAAGCTGGAGTTATTGGAAGTGTTGAAGTAAGTATTTATTGTAATAACTTTTTTAGATTTGGTTTTTCATTTTTCATGAAATAATATTTGGTACTTTGATTCTAACTTTGTGTATTATGATTGTTTTCTTGCAGAGTAATTTGGGTAAATGGTACTATAAGAGCAAACGCCAACCCACTATGCATGAAGGATACATGTTCCCTTTGCTTGTTAGCAAGGAGTTGGATAATTGGCCAGAAATGAACATAAGAAGAAGAAAATGGGTAAGTGTATATGAGACCTATATTCAACTTTTATAATATTCTAATCATGTTTGCGACACGATTCTTGCTATTGCAGAGAATTGCAGTCAAATACAACTTTTGCAACCTCAATCGTAGCCGCTCAGACATAATACTTTTTTATGTCGCAGCGCAGATTGAGGTTGCATACATGATTTTCAAAACCTTGAGCCTAATCAAGTGTCATTTATAAATTATTGTGTCCTATTCAAATGTCAATTCATCTTGCAAATCGCAATATCTTAATTAAGTAGCATTATTGGTTTACTATTATTATTTTTTGTTAGTGTCCTAATATTTCTTTCATTTGTAATCATGCAGTTGACTGTGGATGAAGCAAAAGAAATATGCCCATATGCTTGGATGAAGGAAGCTTTAGATGAATTGGTTAGTAGACAAACCAATTCAATCTAA

Protein sequence

>MS.gene81831.t1

MMCLVSPRTGRHLQRYEQGCRQVVGCIPYRYKKNGTQEKEIEVLLISAQKGNETMEQAALRETIEEAGVIGSVESNLGKWYYKSKRQPTMHEGYMFPLLVSKELDNWPEMNIRRRKWLTVDEAKEICPYAWMKEALDELVSRQTNSI