AlfalfaGEDB | MS.gene050300

M. sativa cultivar XinJiangDaYe / MS.gene050300

KEGG: K10740
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005634 GO:0006139 GO:0006259 GO:0006260 GO:0006281 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0046483 GO:0050896 GO:0051716 GO:0071704 GO:0090304 GO:0097159 GO:0110165 GO:1901360 GO:1901363 GO:1901576
PFAM: PF08661.14

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050300.t1	ABN06078.1	98.1	106	2	0	1	106	54	159	5.70E-51	209.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050300.t1	Q6NLG7	58.5	106	44	0	1	106	1	106	5.1e-32	138.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050300.t1	G7KQW7	98.1	106	2	0	1	106	51	156	4.1e-51	209.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050300	MS.gene056830	0.802267	6.09E-49	-1.69E-46
MS.gene050300	MS.gene058330	-0.802504	5.44E-49	-1.69E-46
MS.gene050300	MS.gene060932	-0.804297	2.31E-49	-1.69E-46
MS.gene050300	MS.gene068350	-0.802832	4.65E-49	-1.69E-46
MS.gene050300	MS.gene072116	-0.810624	1.05E-50	-1.69E-46
MS.gene050300	MS.gene08582	-0.802556	5.30E-49	-1.69E-46
MS.gene050300	MS.gene21103	-0.80678	6.97E-50	-1.69E-46
MS.gene050300	MS.gene21105	-0.80743	5.07E-50	-1.69E-46
MS.gene050300	MS.gene21107	-0.807967	3.90E-50	-1.69E-46
MS.gene050300	MS.gene24696	0.837944	3.85E-57	-1.69E-46
MS.gene050300	MS.gene24731	0.912034	3.09E-83	-1.69E-46
MS.gene050300	MS.gene31650	-0.809775	1.60E-50	-1.69E-46
MS.gene050300	MS.gene33177	-0.809726	1.64E-50	-1.69E-46
MS.gene050300	MS.gene37612	-0.81267	3.77E-51	-1.69E-46
MS.gene050300	MS.gene54045	-0.810724	1.00E-50	-1.69E-46
MS.gene050300	MS.gene54090	-0.80756	4.76E-50	-1.69E-46
MS.gene050300	MS.gene55653	-0.806644	7.44E-50	-1.69E-46
MS.gene050300	MS.gene56803	-0.805608	1.23E-49	-1.69E-46
MS.gene050300	MS.gene56807	0.804249	2.37E-49	-1.69E-46
MS.gene050300	MS.gene57576	-0.808098	3.66E-50	-1.69E-46
MS.gene050300	MS.gene57650	0.808653	2.79E-50	-1.69E-46
MS.gene050300	MS.gene58719	-0.8056	1.23E-49	-1.69E-46
MS.gene050300	MS.gene59540	-0.837963	3.81E-57	-1.69E-46
MS.gene050300	MS.gene60710	-0.810683	1.02E-50	-1.69E-46
MS.gene050300	MS.gene65295	-0.802783	4.76E-49	-1.69E-46
MS.gene050300	MS.gene73078	-0.812286	4.58E-51	-1.69E-46
MS.gene050300	MS.gene74537	-0.803573	3.27E-49	-1.69E-46
MS.gene050300	MS.gene75719	-0.818524	1.87E-52	-1.69E-46
MS.gene050300	MS.gene76314	-0.807206	5.66E-50	-1.69E-46
MS.gene050300	MS.gene77412	-0.800269	1.56E-48	-1.69E-46
MS.gene050300	MS.gene79120	-0.805788	1.13E-49	-1.69E-46
MS.gene050300	MS.gene80631	-0.809474	1.86E-50	-1.69E-46
MS.gene050300	MS.gene81831	-0.801992	6.93E-49	-1.69E-46
MS.gene050300	MS.gene87673	-0.804221	2.40E-49	-1.69E-46
MS.gene050300	MS.gene87922	-0.818555	1.84E-52	-1.69E-46
MS.gene050300	MS.gene88147	-0.809578	1.77E-50	-1.69E-46
MS.gene050300	MS.gene89985	-0.810559	1.09E-50	-1.69E-46
MS.gene050300	MS.gene92775	-0.834977	2.19E-56	-1.69E-46
MS.gene050300	MS.gene94674	-0.801442	8.99E-49	-1.69E-46
MS.gene050300	MS.gene95655	-0.81222	4.73E-51	-1.69E-46
MS.gene050300	MS.gene96973	-0.800911	1.15E-48	-1.69E-46
MS.gene050300	MS.gene98152	0.810472	1.13E-50	-1.69E-46
MS.gene050300	MS.gene99483	-0.802063	6.70E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050300.t1	MTR_7g104870	98.113	106	2	0	1	106	51	156	6.83e-74	216
MS.gene050300.t1	MTR_1g112020	54.206	107	48	1	1	106	1	107	2.60e-38	124
MS.gene050300.t1	MTR_1g112040	55.140	107	47	1	1	106	1	107	9.06e-38	123

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene050300.t1	AT4G18590	58.491	106	44	0	1	106	1	106	2.64e-46	144
MS.gene050300.t1	AT3G52630	55.140	107	47	1	1	106	1	107	7.44e-42	133
MS.gene050300.t1	AT3G52630	55.140	107	47	1	1	106	1	107	7.44e-42	133

Find 22 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACCCTTCTTCCGACATAAA+AGG	0.241109	7.2:+8075880	None:intergenic
ACAAGGACTGCAGGGCTTAA+TGG	0.275580	7.2:+8075920	None:intergenic
GAGAAGCAGTTGATTGTAAA+AGG	0.288767	7.2:-8075797	MS.gene050300:CDS
ACCAACTTTGGTGATGAAAT+TGG	0.344480	7.2:-8075692	MS.gene050300:intron
TTGCAAGCTGACATAGCTTA+TGG	0.355631	7.2:+8075486	None:intergenic
GGTCCATAACTCAGCTTTGA+TGG	0.369681	7.2:+8075712	None:intergenic
ACAATCAACTGCTTCTCATC+TGG	0.376539	7.2:+8075803	None:intergenic
ACCAATTTCATCACCAAAGT+TGG	0.430140	7.2:+8075691	None:intergenic
ACAAAACTTGTAAGAGGAGC+AGG	0.439485	7.2:+8075767	None:intergenic
ACTTCTACAAAACTTGTAAG+AGG	0.459678	7.2:+8075761	None:intergenic
GCTGAGTTATGGACCAACTT+TGG	0.463853	7.2:-8075704	MS.gene050300:CDS
TCTGCATTGACAAGGACTGC+AGG	0.468536	7.2:+8075911	None:intergenic
ACCCTTCTTCCGACATAAAA+GGG	0.468915	7.2:+8075881	None:intergenic
AAGCTATGTCAGCTTGCAAA+TGG	0.472567	7.2:-8075482	MS.gene050300:CDS
TCTGACGCTCTAGCTGTCAT+TGG	0.479697	7.2:-8075833	MS.gene050300:CDS
AGTCCATCAAAGCTGAGTTA+TGG	0.498331	7.2:-8075715	MS.gene050300:CDS
AAGGGTTCGTACAGTGATGC+AGG	0.527340	7.2:-8075864	MS.gene050300:CDS
CTGCATTGACAAGGACTGCA+GGG	0.550141	7.2:+8075912	None:intergenic
CAAAACTTGTAAGAGGAGCA+GGG	0.615820	7.2:+8075768	None:intergenic
AAAACTTGTAAGAGGAGCAG+GGG	0.643901	7.2:+8075769	None:intergenic
GCAACAGTTCTGCATTGACA+AGG	0.657999	7.2:+8075903	None:intergenic
AAACTTGTAAGAGGAGCAGG+GGG	0.714728	7.2:+8075770	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTAATGATATTTATTCTCTA+TGG	-	chr7.2:8075784-8075803	MS.gene050300:CDS	20.0%
!!	TTGAAATTTCACTTATAACA+AGG	+	chr7.2:8075811-8075830	None:intergenic	20.0%
!!!	ACAAGTTTTGTAGAAGTTAT+TGG	-	chr7.2:8075628-8075647	MS.gene050300:intron	25.0%
	ACTTCTACAAAACTTGTAAG+AGG	+	chr7.2:8075625-8075644	None:intergenic	30.0%
	TTCTAAGCATATTAAGCTGT+GGG	-	chr7.2:8075833-8075852	MS.gene050300:CDS	30.0%
	TTTCTAAGCATATTAAGCTG+TGG	-	chr7.2:8075832-8075851	MS.gene050300:CDS	30.0%
!	TTTATTCCCTTTTCTCAAAC+TGG	-	chr7.2:8075722-8075741	MS.gene050300:CDS	30.0%
	ACCAATTTCATCACCAAAGT+TGG	+	chr7.2:8075695-8075714	None:intergenic	35.0%
	ATGACAACCAGTTTGAGAAA+AGG	+	chr7.2:8075732-8075751	None:intergenic	35.0%
	GAGAAGCAGTTGATTGTAAA+AGG	-	chr7.2:8075586-8075605	MS.gene050300:intron	35.0%
	TGACAACCAGTTTGAGAAAA+GGG	+	chr7.2:8075731-8075750	None:intergenic	35.0%
!!	ACCAACTTTGGTGATGAAAT+TGG	-	chr7.2:8075691-8075710	MS.gene050300:intron	35.0%
	AAAACTTGTAAGAGGAGCAG+GGG	+	chr7.2:8075617-8075636	None:intergenic	40.0%
	AACCCTTCTTCCGACATAAA+AGG	+	chr7.2:8075506-8075525	None:intergenic	40.0%
	AAGCTATGTCAGCTTGCAAA+TGG	-	chr7.2:8075901-8075920	MS.gene050300:CDS	40.0%
	ACAAAACTTGTAAGAGGAGC+AGG	+	chr7.2:8075619-8075638	None:intergenic	40.0%
	ACAATCAACTGCTTCTCATC+TGG	+	chr7.2:8075583-8075602	None:intergenic	40.0%
	ACCCTTCTTCCGACATAAAA+GGG	+	chr7.2:8075505-8075524	None:intergenic	40.0%
	AGTCCATCAAAGCTGAGTTA+TGG	-	chr7.2:8075668-8075687	MS.gene050300:intron	40.0%
	CAAAACTTGTAAGAGGAGCA+GGG	+	chr7.2:8075618-8075637	None:intergenic	40.0%
	TTGCAAGCTGACATAGCTTA+TGG	+	chr7.2:8075900-8075919	None:intergenic	40.0%
!	GAACTGTTGCCCTTTTATGT+CGG	-	chr7.2:8075493-8075512	MS.gene050300:CDS	40.0%
	AAACTTGTAAGAGGAGCAGG+GGG	+	chr7.2:8075616-8075635	None:intergenic	45.0%
	GCTGAGTTATGGACCAACTT+TGG	-	chr7.2:8075679-8075698	MS.gene050300:intron	45.0%
!	GCAACAGTTCTGCATTGACA+AGG	+	chr7.2:8075483-8075502	None:intergenic	45.0%
!	GCCCTTTTATGTCGGAAGAA+GGG	-	chr7.2:8075501-8075520	MS.gene050300:CDS	45.0%
!	GGTCCATAACTCAGCTTTGA+TGG	+	chr7.2:8075674-8075693	None:intergenic	45.0%
!	TGCCCTTTTATGTCGGAAGA+AGG	-	chr7.2:8075500-8075519	MS.gene050300:CDS	45.0%
	AAGGGTTCGTACAGTGATGC+AGG	-	chr7.2:8075519-8075538	MS.gene050300:intron	50.0%
	ACAAGGACTGCAGGGCTTAA+TGG	+	chr7.2:8075466-8075485	None:intergenic	50.0%
	TCTGACGCTCTAGCTGTCAT+TGG	-	chr7.2:8075550-8075569	MS.gene050300:intron	50.0%
!	CTGCATTGACAAGGACTGCA+GGG	+	chr7.2:8075474-8075493	None:intergenic	50.0%
!	TCTGCATTGACAAGGACTGC+AGG	+	chr7.2:8075475-8075494	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	8075457	8075948	8075457	ID=MS.gene050300
chr7.2	mRNA	8075457	8075948	8075457	ID=MS.gene050300.t1;Parent=MS.gene050300
chr7.2	exon	8075693	8075948	8075693	ID=MS.gene050300.t1.exon1;Parent=MS.gene050300.t1
chr7.2	CDS	8075693	8075948	8075693	ID=cds.MS.gene050300.t1;Parent=MS.gene050300.t1
chr7.2	exon	8075457	8075521	8075457	ID=MS.gene050300.t1.exon2;Parent=MS.gene050300.t1
chr7.2	CDS	8075457	8075521	8075457	ID=cds.MS.gene050300.t1;Parent=MS.gene050300.t1

Gene Sequence

>MS.gene050300

ATGGATCCATTAAGCCCTGCAGTCCTTGTCAATGCAGAACTGTTGCCCTTTTATGTCGGAAGAAGGGTTCGTACAGTGATGCAGGTTGTGCGATCTGACGCTCTAGCTGTCATTGGAAAATCACCAGATGAGAAGCAGTTGATTGTAAAAGGATCACCCCCTGCTCCTCTTACAAGTTTTGTAGAAGTTATTGGTATTGTTAACAGTGACAAGTCCATCAAAGCTGAGTTATGGACCAACTTTGGTGATGAAATTGGTATTGTTTTTTATTCCCTTTTCTCAAACTGGTTGTCATATTGATTTTGAGACATGACTCTTAAACTTAATGTAATGATATTTATTCTCTATGGTCCTTGTTATAAGTGAAATTTCAAATTTCTAAGCATATTAAGCTGTGGGTTTTGATATTGTTTCAATGCTTTTGCAGATATGCTCAGCTACCATAAGCTATGTCAGCTTGCAAATGGAGAATTTAAACACTTGTTCCTGTGA

Protein sequence

>MS.gene050300.t1

MDPLSPAVLVNAELLPFYVGRRVRTVMQVVRSDALAVIGKSPDEKQLIVKGSPPAPLTSFVEVIGIVNSDKSIKAELWTNFGDEIDMLSYHKLCQLANGEFKHLFL