AlfalfaGEDB | MS.gene82827

M. sativa cultivar XinJiangDaYe / MS.gene82827

KEGG: K02948
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00411.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82827.t1	XP_013450760.1	97.6	205	3	1	23	225	33	237	1.30E-105	392.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82827.t1	Q8VZT8	58.7	167	61	2	64	225	151	314	1.5e-49	197.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82827.t1	I3SP73	97.6	205	3	1	23	225	33	237	9.5e-106	392.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene82827	0.805467	1.32E-49	-1.69E-46
MS.gene049382	MS.gene82827	0.816894	4.37E-52	-1.69E-46
MS.gene050277	MS.gene82827	0.879212	1.50E-69	-1.69E-46
MS.gene050320	MS.gene82827	0.819414	1.18E-52	-1.69E-46
MS.gene051063	MS.gene82827	0.850455	1.68E-60	-1.69E-46
MS.gene051245	MS.gene82827	0.853994	1.65E-61	-1.69E-46
MS.gene05134	MS.gene82827	0.806028	1.00E-49	-1.69E-46
MS.gene051633	MS.gene82827	0.823015	1.74E-53	-1.69E-46
MS.gene051746	MS.gene82827	0.829886	4.00E-55	-1.69E-46
MS.gene051748	MS.gene82827	0.824592	7.43E-54	-1.69E-46
MS.gene051757	MS.gene82827	0.804623	1.98E-49	-1.69E-46
MS.gene051903	MS.gene82827	0.81133	7.39E-51	-1.69E-46
MS.gene052286	MS.gene82827	0.844626	6.73E-59	-1.69E-46
MS.gene052302	MS.gene82827	0.806398	8.39E-50	-1.69E-46
MS.gene052376	MS.gene82827	0.801336	9.45E-49	-1.69E-46
MS.gene053109	MS.gene82827	0.861674	8.69E-64	-1.69E-46
MS.gene055133	MS.gene82827	0.813748	2.19E-51	-1.69E-46
MS.gene055162	MS.gene82827	0.845312	4.39E-59	-1.69E-46
MS.gene056069	MS.gene82827	-0.833243	5.97E-56	-1.69E-46
MS.gene056432	MS.gene82827	0.802627	5.13E-49	-1.69E-46
MS.gene056601	MS.gene82827	0.827869	1.23E-54	-1.69E-46
MS.gene056631	MS.gene82827	0.856156	3.89E-62	-1.69E-46
MS.gene057057	MS.gene82827	0.824913	6.24E-54	-1.69E-46
MS.gene057140	MS.gene82827	0.808091	3.67E-50	-1.69E-46
MS.gene057234	MS.gene82827	0.84711	1.42E-59	-1.69E-46
MS.gene058182	MS.gene82827	0.818366	2.03E-52	-1.69E-46
MS.gene058201	MS.gene82827	0.815658	8.26E-52	-1.69E-46
MS.gene058238	MS.gene82827	0.810488	1.12E-50	-1.69E-46
MS.gene058906	MS.gene82827	0.87325	1.70E-67	-1.69E-46
MS.gene059898	MS.gene82827	0.860004	2.79E-63	-1.69E-46
MS.gene06001	MS.gene82827	0.839845	1.24E-57	-1.69E-46
MS.gene061145	MS.gene82827	0.832222	1.07E-55	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82827.t1	MTR_6g004800	97.561	205	3	1	23	225	33	237	5.66e-146	406
MS.gene82827.t1	MTR_2g103160	89.806	206	18	2	23	225	33	238	8.30e-133	373

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene82827.t1	AT1G31817	59.394	165	59	2	66	225	153	314	3.85e-65	204

Find 49 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGGCAGTCTGCAGCCATTA+TGG	0.258176	6.1:-922258	None:intergenic
AATCAGGGCAAAAGCTTTCT+AGG	0.277751	6.1:+922038	MS.gene82827:CDS
CCCTGATTTCATGTCTTTCA+AGG	0.289130	6.1:-922023	None:intergenic
GGACGGAGATCTAGAGGTTT+AGG	0.305182	6.1:+922091	MS.gene82827:CDS
TGGTGGAAATTCCAGGAAAA+TGG	0.320222	6.1:+921826	MS.gene82827:CDS
GAAGATGAAAAGAGTGTTAT+GGG	0.321530	6.1:+921869	MS.gene82827:CDS
TGGCAGTCTGCAGCCATTAT+GGG	0.322578	6.1:-922257	None:intergenic
AGAAGATGAAAAGAGTGTTA+TGG	0.333476	6.1:+921868	MS.gene82827:CDS
TCCTTGAAAGACATGAAATC+AGG	0.342062	6.1:+922022	MS.gene82827:CDS
AACTAGATACGTCGACTCTT+TGG	0.342449	6.1:-922277	None:intergenic
GAAACTGCAGGATATGTTAG+AGG	0.388568	6.1:+921677	MS.gene82827:intron
CTGAGATTGGTGGAAATTCC+AGG	0.399094	6.1:+921819	MS.gene82827:CDS
GTATCTTCAATGTATACAAT+TGG	0.413544	6.1:-922223	None:intergenic
GTAAAACTTAGTGGCTCTGC+TGG	0.418753	6.1:+921998	MS.gene82827:CDS
TCCCGACCCCGCAGAAAATC+CGG	0.427991	6.1:-921767	None:intergenic
ACAACTCGTCGTCCCCATAA+TGG	0.428444	6.1:+922244	MS.gene82827:CDS
AAAGGAAATGTAAAACTTAG+TGG	0.428546	6.1:+921989	MS.gene82827:CDS
TGTGAATGCAAGTGCTCGTC+CGG	0.438860	6.1:+921748	MS.gene82827:CDS
GTTACAGTGACCGATTCTAA+AGG	0.474490	6.1:+921971	MS.gene82827:CDS
GTGCAAGTTGGGGTTTGAGC+GGG	0.480718	6.1:+921708	MS.gene82827:CDS
CATCATGCTGGAAATGATAC+AGG	0.497731	6.1:-921907	None:intergenic
TGCTGAAGCAACTGCGGAAG+TGG	0.498444	6.1:+922066	MS.gene82827:CDS
AGAATCGGTCACTGTAACAA+AGG	0.511601	6.1:-921966	None:intergenic
GAACAAAATCAGCATCATGC+TGG	0.519192	6.1:-921919	None:intergenic
GAGAATAATGCTGAGATTGG+TGG	0.526522	6.1:+921809	MS.gene82827:CDS
AGTGAGAATAATGCTGAGAT+TGG	0.539267	6.1:+921806	MS.gene82827:CDS
CAAGTTGGGGTTTGAGCGGG+AGG	0.553465	6.1:+921711	MS.gene82827:CDS
GGCAGTCTGCAGCCATTATG+GGG	0.556364	6.1:-922256	None:intergenic
GAAGCAACTGCGGAAGTGGT+TGG	0.563081	6.1:+922070	MS.gene82827:CDS
AGCAATATTGAGCTGGAGGG+AGG	0.568183	6.1:+922171	MS.gene82827:CDS
AAAGGCAAGCAATATTGAGC+TGG	0.578900	6.1:+922164	MS.gene82827:CDS
GTGGTTGGACGGAGATCTAG+AGG	0.582119	6.1:+922085	MS.gene82827:CDS
CCTTGAAAGACATGAAATCA+GGG	0.583578	6.1:+922023	MS.gene82827:CDS
AGTGCAAGTTGGGGTTTGAG+CGG	0.585051	6.1:+921707	MS.gene82827:CDS
GCAATATTGAGCTGGAGGGA+GGG	0.589094	6.1:+922172	MS.gene82827:CDS
CAACTGCGGAAGTGGTTGGA+CGG	0.593288	6.1:+922074	MS.gene82827:CDS
TCTGTTGTCATGAAAGTGAA+CGG	0.595836	6.1:+922121	MS.gene82827:CDS
TGTGAAAAGAAGAGTGAAGA+TGG	0.596358	6.1:+921497	MS.gene82827:CDS
AGGAAAATGGACTTTGTGAG+AGG	0.596525	6.1:+921839	MS.gene82827:CDS
TCACATAACGCAGCAGGCAA+AGG	0.597151	6.1:-921479	None:intergenic
GGCAAGCAATATTGAGCTGG+AGG	0.597786	6.1:+922167	MS.gene82827:CDS
GAATGCATGGTAATAGAGAG+AGG	0.622714	6.1:+921450	None:intergenic
AATGCATGGTAATAGAGAGA+GGG	0.623514	6.1:+921451	None:intergenic
GAGGGATTTACTGATTCTCG+AGG	0.625322	6.1:+922190	MS.gene82827:CDS
GCAAGCAATATTGAGCTGGA+GGG	0.647783	6.1:+922168	MS.gene82827:CDS
AGTGAAGATGAATGAATGCA+TGG	0.656477	6.1:+921437	None:intergenic
GTATGCTGCTGAAGCAACTG+CGG	0.658519	6.1:+922060	MS.gene82827:CDS
ATGCATGGTAATAGAGAGAG+GGG	0.667896	6.1:+921452	MS.gene82827:CDS
GATGAAAAGAGTGTTATGGG+AGG	0.673626	6.1:+921872	MS.gene82827:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTGTTCATATAAAGATG+CGG	+	chr6.1:921933-921952	MS.gene82827:CDS	20.0%
!!!	TTTTACATTTCCTTTAGAAT+CGG	-	chr6.1:921984-922003	None:intergenic	20.0%
!	AAAGGAAATGTAAAACTTAG+TGG	+	chr6.1:921989-922008	MS.gene82827:CDS	25.0%
!	AATCACACAAAACCTTATTA+GGG	-	chr6.1:921528-921547	None:intergenic	25.0%
!	GTATCTTCAATGTATACAAT+TGG	-	chr6.1:922226-922245	None:intergenic	25.0%
!	TAATCACACAAAACCTTATT+AGG	-	chr6.1:921529-921548	None:intergenic	25.0%
!	TTATGCAATGACATGAAATA+TGG	-	chr6.1:921587-921606	None:intergenic	25.0%
	ATCACACAAAACCTTATTAG+GGG	-	chr6.1:921527-921546	None:intergenic	30.0%
!	TTACTCATTTTCGAAGGAAA+AGG	+	chr6.1:922146-922165	MS.gene82827:CDS	30.0%
!!	AGAAGATGAAAAGAGTGTTA+TGG	+	chr6.1:921868-921887	MS.gene82827:CDS	30.0%
!!	GAAGATGAAAAGAGTGTTAT+GGG	+	chr6.1:921869-921888	MS.gene82827:CDS	30.0%
	AGTGAGAATAATGCTGAGAT+TGG	+	chr6.1:921806-921825	MS.gene82827:CDS	35.0%
	CCTTGAAAGACATGAAATCA+GGG	+	chr6.1:922023-922042	MS.gene82827:CDS	35.0%
	TCCTTGAAAGACATGAAATC+AGG	+	chr6.1:922022-922041	MS.gene82827:CDS	35.0%
	TCTGTTGTCATGAAAGTGAA+CGG	+	chr6.1:922121-922140	MS.gene82827:CDS	35.0%
	TGTGAAAAGAAGAGTGAAGA+TGG	+	chr6.1:921497-921516	MS.gene82827:CDS	35.0%
	TTTCATACTCTTGAAACTGC+AGG	+	chr6.1:921665-921684	MS.gene82827:intron	35.0%
!	ACGGATTTACTCATTTTCGA+AGG	+	chr6.1:922140-922159	MS.gene82827:CDS	35.0%
	AAAGGCAAGCAATATTGAGC+TGG	+	chr6.1:922164-922183	MS.gene82827:CDS	40.0%
	AAGATGGTTGACCCCTAATA+AGG	+	chr6.1:921513-921532	MS.gene82827:intron	40.0%
	AATCAGGGCAAAAGCTTTCT+AGG	+	chr6.1:922038-922057	MS.gene82827:CDS	40.0%
	AGAATCGGTCACTGTAACAA+AGG	-	chr6.1:921969-921988	None:intergenic	40.0%
	ATGCATGGTAATAGAGAGAG+GGG	+	chr6.1:921452-921471	MS.gene82827:CDS	40.0%
	CATCATGCTGGAAATGATAC+AGG	-	chr6.1:921910-921929	None:intergenic	40.0%
	CCCTGATTTCATGTCTTTCA+AGG	-	chr6.1:922026-922045	None:intergenic	40.0%
	GAAACTGCAGGATATGTTAG+AGG	+	chr6.1:921677-921696	MS.gene82827:intron	40.0%
	GAACAAAATCAGCATCATGC+TGG	-	chr6.1:921922-921941	None:intergenic	40.0%
	GAGAATAATGCTGAGATTGG+TGG	+	chr6.1:921809-921828	MS.gene82827:CDS	40.0%
	TGGTGGAAATTCCAGGAAAA+TGG	+	chr6.1:921826-921845	MS.gene82827:CDS	40.0%
!	AGGAAAATGGACTTTGTGAG+AGG	+	chr6.1:921839-921858	MS.gene82827:CDS	40.0%
!	GTTACAGTGACCGATTCTAA+AGG	+	chr6.1:921971-921990	MS.gene82827:CDS	40.0%
!	TCTCACAAAGTCCATTTTCC+TGG	-	chr6.1:921840-921859	None:intergenic	40.0%
!	TTCTTTTCACATAACGCAGC+AGG	-	chr6.1:921488-921507	None:intergenic	40.0%
!!	GATGAAAAGAGTGTTATGGG+AGG	+	chr6.1:921872-921891	MS.gene82827:CDS	40.0%
!!!	AGGATTTTCGAGTGCAAGTT+GGG	+	chr6.1:921697-921716	MS.gene82827:CDS	40.0%
	CTGAGATTGGTGGAAATTCC+AGG	+	chr6.1:921819-921838	MS.gene82827:CDS	45.0%
	GCAAGCAATATTGAGCTGGA+GGG	+	chr6.1:922168-922187	MS.gene82827:CDS	45.0%
!	GTAAAACTTAGTGGCTCTGC+TGG	+	chr6.1:921998-922017	MS.gene82827:CDS	45.0%
!!	GAGGGATTTACTGATTCTCG+AGG	+	chr6.1:922190-922209	MS.gene82827:CDS	45.0%
!!!	GAGGATTTTCGAGTGCAAGT+TGG	+	chr6.1:921696-921715	MS.gene82827:CDS	45.0%
!!!	GGATTTTCGAGTGCAAGTTG+GGG	+	chr6.1:921698-921717	MS.gene82827:CDS	45.0%
	ACAACTCGTCGTCCCCATAA+TGG	+	chr6.1:922244-922263	MS.gene82827:CDS	50.0%
	AGCAATATTGAGCTGGAGGG+AGG	+	chr6.1:922171-922190	MS.gene82827:CDS	50.0%
	GCAATATTGAGCTGGAGGGA+GGG	+	chr6.1:922172-922191	MS.gene82827:CDS	50.0%
	GGACGGAGATCTAGAGGTTT+AGG	+	chr6.1:922091-922110	MS.gene82827:CDS	50.0%
	GGCAAGCAATATTGAGCTGG+AGG	+	chr6.1:922167-922186	MS.gene82827:CDS	50.0%
	GTATGCTGCTGAAGCAACTG+CGG	+	chr6.1:922060-922079	MS.gene82827:CDS	50.0%
	TCACATAACGCAGCAGGCAA+AGG	-	chr6.1:921482-921501	None:intergenic	50.0%
	TGGCAGTCTGCAGCCATTAT+GGG	-	chr6.1:922260-922279	None:intergenic	50.0%
	TGTGAATGCAAGTGCTCGTC+CGG	+	chr6.1:921748-921767	MS.gene82827:CDS	50.0%
	TTGGCAGTCTGCAGCCATTA+TGG	-	chr6.1:922261-922280	None:intergenic	50.0%
!	AGTGCAAGTTGGGGTTTGAG+CGG	+	chr6.1:921707-921726	MS.gene82827:CDS	50.0%
	CAACTGCGGAAGTGGTTGGA+CGG	+	chr6.1:922074-922093	MS.gene82827:CDS	55.0%
	GAAGCAACTGCGGAAGTGGT+TGG	+	chr6.1:922070-922089	MS.gene82827:CDS	55.0%
	GGCAGTCTGCAGCCATTATG+GGG	-	chr6.1:922259-922278	None:intergenic	55.0%
	GTGGTTGGACGGAGATCTAG+AGG	+	chr6.1:922085-922104	MS.gene82827:CDS	55.0%
	TGCTGAAGCAACTGCGGAAG+TGG	+	chr6.1:922066-922085	MS.gene82827:CDS	55.0%
!	GTGCAAGTTGGGGTTTGAGC+GGG	+	chr6.1:921708-921727	MS.gene82827:CDS	55.0%
!	GTGCTCGTCCGGATTTTCTG+CGG	+	chr6.1:921759-921778	MS.gene82827:CDS	55.0%
!	TGCTCGTCCGGATTTTCTGC+GGG	+	chr6.1:921760-921779	MS.gene82827:CDS	55.0%
	TCCCGACCCCGCAGAAAATC+CGG	-	chr6.1:921770-921789	None:intergenic	60.0%
!	CAAGTTGGGGTTTGAGCGGG+AGG	+	chr6.1:921711-921730	MS.gene82827:CDS	60.0%
!	GCTCGTCCGGATTTTCTGCG+GGG	+	chr6.1:921761-921780	MS.gene82827:CDS	60.0%
!	GTCCGGATTTTCTGCGGGGT+CGG	+	chr6.1:921765-921784	MS.gene82827:CDS	60.0%
!	TCCGGATTTTCTGCGGGGTC+GGG	+	chr6.1:921766-921785	MS.gene82827:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	921452	922297	921452	ID=MS.gene82827
chr6.1	mRNA	921452	922297	921452	ID=MS.gene82827.t1;Parent=MS.gene82827
chr6.1	exon	921452	921518	921452	ID=MS.gene82827.t1.exon1;Parent=MS.gene82827.t1
chr6.1	CDS	921452	921518	921452	ID=cds.MS.gene82827.t1;Parent=MS.gene82827.t1
chr6.1	exon	921687	922297	921687	ID=MS.gene82827.t1.exon2;Parent=MS.gene82827.t1
chr6.1	CDS	921687	922297	921687	ID=cds.MS.gene82827.t1;Parent=MS.gene82827.t1

Gene Sequence

>MS.gene82827

ATGCATGGTAATAGAGAGAGGGGCATACCTTTGCCTGCTGCGTTATGTGAAAAGAAGAGTGAAGATGGTTGACCCCTAATAAGGTTTTGTGTGATTATAAATTGTAAATTGTGAAACATGTGTTTATTATGACCATATTTCATGTCATTGCATAATTAAATACATATATCATTTTGTTTTTTACTGTTTTTTTCATGTCATCTCAACAATCACTTTCATACTCTTGAAACTGCAGGATATGTTAGAGGATTTTCGAGTGCAAGTTGGGGTTTGAGCGGGAGGAATCAAAGTGAGAATGTGAATGCAAGTGCTCGTCCGGATTTTCTGCGGGGTCGGGAGAATATTAACTTCAAAAGTGAGAATAATGCTGAGATTGGTGGAAATTCCAGGAAAATGGACTTTGTGAGAGGAGCTATAGAAGATGAAAAGAGTGTTATGGGAGGTTATCTATACAACCTGTATCATTTCCAGCATGATGCTGATTTTGTTCATATAAAGATGCGGCGCAACAATACCTTTGTTACAGTGACCGATTCTAAAGGAAATGTAAAACTTAGTGGCTCTGCTGGTTCCTTGAAAGACATGAAATCAGGGCAAAAGCTTTCTAGGTATGCTGCTGAAGCAACTGCGGAAGTGGTTGGACGGAGATCTAGAGGTTTAGGTTTGAAATCTGTTGTCATGAAAGTGAACGGATTTACTCATTTTCGAAGGAAAAGGCAAGCAATATTGAGCTGGAGGGAGGGATTTACTGATTCTCGAGGAGATAAAAATCCAATTGTATACATTGAAGATACAACTCGTCGTCCCCATAATGGCTGCAGACTGCCAAAGAGTCGACGTATCTAG

Protein sequence

>MS.gene82827.t1

MHGNRERGIPLPAALCEKKSEDGYVRGFSSASWGLSGRNQSENVNASARPDFLRGRENINFKSENNAEIGGNSRKMDFVRGAIEDEKSVMGGYLYNLYHFQHDADFVHIKMRRNNTFVTVTDSKGNVKLSGSAGSLKDMKSGQKLSRYAAEATAEVVGRRSRGLGLKSVVMKVNGFTHFRRKRQAILSWREGFTDSRGDKNPIVYIEDTTRRPHNGCRLPKSRRI