AlfalfaGEDB | MS.gene83357

M. sativa cultivar XinJiangDaYe / MS.gene83357

KEGG: K14769
GO: GO:0005575 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016070 GO:0016072 GO:0030684 GO:0032040 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0043170 GO:0044237 GO:0044238 GO:0046483 GO:0071704 GO:0090304 GO:1901360 GO:1990904
PFAM: PF03998.16

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83357.t1	XP_021811126.1	56.6	143	33	3	1	114	1	143	1.80E-29	138.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83357.t1	Q8S1Z1	52.1	140	38	3	1	111	1	140	9.9e-29	127.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83357.t1	A0A1S2XSG9	55.5	137	32	1	1	108	1	137	2.9e-29	137.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene83357	0.803135	4.03E-49	-1.69E-46
MS.gene049272	MS.gene83357	0.824666	7.13E-54	-1.69E-46
MS.gene049501	MS.gene83357	0.815243	1.02E-51	-1.69E-46
MS.gene049669	MS.gene83357	0.803489	3.40E-49	-1.69E-46
MS.gene049850	MS.gene83357	0.826518	2.60E-54	-1.69E-46
MS.gene050226	MS.gene83357	0.812604	3.90E-51	-1.69E-46
MS.gene050227	MS.gene83357	0.814955	1.18E-51	-1.69E-46
MS.gene050228	MS.gene83357	0.816716	4.79E-52	-1.69E-46
MS.gene050442	MS.gene83357	0.832836	7.53E-56	-1.69E-46
MS.gene050719	MS.gene83357	0.804246	2.37E-49	-1.69E-46
MS.gene050721	MS.gene83357	0.807075	6.03E-50	-1.69E-46
MS.gene050759	MS.gene83357	0.809339	1.99E-50	-1.69E-46
MS.gene050844	MS.gene83357	0.804576	2.02E-49	-1.69E-46
MS.gene051210	MS.gene83357	0.810382	1.19E-50	-1.69E-46
MS.gene052012	MS.gene83357	0.827973	1.16E-54	-1.69E-46
MS.gene052384	MS.gene83357	0.955619	2.00E-113	-1.69E-46
MS.gene052519	MS.gene83357	0.806994	6.28E-50	-1.69E-46
MS.gene052582	MS.gene83357	0.804156	2.47E-49	-1.69E-46
MS.gene052584	MS.gene83357	0.801721	7.88E-49	-1.69E-46
MS.gene052896	MS.gene83357	0.808433	3.10E-50	-1.69E-46
MS.gene053008	MS.gene83357	0.810749	9.87E-51	-1.69E-46
MS.gene053226	MS.gene83357	0.808068	3.71E-50	-1.69E-46
MS.gene054291	MS.gene83357	-0.804771	1.84E-49	-1.69E-46
MS.gene054468	MS.gene83357	0.808487	3.02E-50	-1.69E-46
MS.gene055701	MS.gene83357	0.801556	8.52E-49	-1.69E-46
MS.gene055818	MS.gene83357	0.826778	2.25E-54	-1.69E-46
MS.gene056162	MS.gene83357	0.820343	7.21E-53	-1.69E-46
MS.gene056506	MS.gene83357	0.801605	8.32E-49	-1.69E-46
MS.gene057026	MS.gene83357	0.803593	3.24E-49	-1.69E-46
MS.gene058548	MS.gene83357	0.807374	5.21E-50	-1.69E-46
MS.gene058625	MS.gene83357	0.82339	1.42E-53	-1.69E-46
MS.gene058829	MS.gene83357	0.823981	1.03E-53	-1.69E-46
MS.gene060011	MS.gene83357	0.808697	2.73E-50	-1.69E-46
MS.gene060322	MS.gene83357	0.801896	7.25E-49	-1.69E-46
MS.gene06052	MS.gene83357	0.806426	8.27E-50	-1.69E-46
MS.gene060572	MS.gene83357	0.812235	4.70E-51	-1.69E-46
MS.gene060690	MS.gene83357	0.812017	5.24E-51	-1.69E-46
MS.gene060781	MS.gene83357	0.800403	1.46E-48	-1.69E-46
MS.gene060803	MS.gene83357	0.815974	7.02E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83357.t1	MTR_4g094380	53.521	142	37	3	1	113	1	142	4.43e-42	136
MS.gene83357.t1	MTR_4g094380	52.143	140	38	3	1	111	1	140	1.32e-38	129

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83357.t1	AT3G60360	47.794	136	43	3	1	108	1	136	3.40e-33	115

Find 17 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGATTCAGTCTGAGAAAA+AGG	0.320386	7.3:+13690702	MS.gene83357:CDS
ATCTTAAAGTAAAATTCATC+TGG	0.330226	7.3:-13690586	None:intergenic
AGGGTTTCACGGAAACGATT+TGG	0.362984	7.3:+13690363	MS.gene83357:intron
ATTAGAGCTAGACTTGTTGA+TGG	0.367637	7.3:+13690610	MS.gene83357:CDS
GCTTGTCTGTCAGACACTTA+AGG	0.375501	7.3:+13690543	MS.gene83357:intron
AAATCAACTCATGCTTGTTC+TGG	0.381745	7.3:-13690905	None:intergenic
CAGACTGAATCTCTTGAAGA+AGG	0.417743	7.3:-13690693	None:intergenic
GTCTACGGATGATGTTTCTC+AGG	0.432159	7.3:-13690226	None:intergenic
GCACAAAGACTATCTCCTTC+GGG	0.434793	7.3:+13690398	MS.gene83357:CDS
AGCACAAAGACTATCTCCTT+CGG	0.447683	7.3:+13690397	MS.gene83357:CDS
TCGTAACTATTAGGGTTTCA+CGG	0.494684	7.3:+13690352	MS.gene83357:intron
TCTTAAAGTAAAATTCATCT+GGG	0.525519	7.3:-13690585	None:intergenic
TGTGATAAGCTTTAGCCCGA+AGG	0.563386	7.3:-13690413	None:intergenic
AGACTGAATCTCTTGAAGAA+GGG	0.587041	7.3:-13690692	None:intergenic
AATAGCATGAGAATTCAGTT+CGG	0.612076	7.3:-13690855	None:intergenic
GCTCTTTGTAAGTACGTCTA+CGG	0.634635	7.3:-13690241	None:intergenic
TCTACGGATGATGTTTCTCA+GGG	0.657071	7.3:-13690225	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATCTTAAAGTAAAATTCATC+TGG	-	chr7.3:13690589-13690608	None:intergenic	20.0%
!!	TCTTAAAGTAAAATTCATCT+GGG	-	chr7.3:13690588-13690607	None:intergenic	20.0%
!	TTCATGCTTAGTTTCAAATT+TGG	+	chr7.3:13690470-13690489	MS.gene83357:intron	25.0%
!!!	GTTTGTTTTCGTAACTATTA+GGG	+	chr7.3:13690344-13690363	MS.gene83357:intron	25.0%
!!!	TTCTCATGCTATTTTCTATA+CGG	+	chr7.3:13690865-13690884	MS.gene83357:CDS	25.0%
	AATAGCATGAGAATTCAGTT+CGG	-	chr7.3:13690858-13690877	None:intergenic	30.0%
	ACAAACGAAATTGAAGCATA+TGG	-	chr7.3:13690330-13690349	None:intergenic	30.0%
	CATTCACAAGTAAGCAAATT+TGG	-	chr7.3:13690795-13690814	None:intergenic	30.0%
	TAAAGCTTATCACAAAAAGC+AGG	+	chr7.3:13690422-13690441	MS.gene83357:CDS	30.0%
!	TTTTCAGTTTTCTGCAAGAA+GGG	-	chr7.3:13690827-13690846	None:intergenic	30.0%
!!	CGTTTGTTTTCGTAACTATT+AGG	+	chr7.3:13690343-13690362	MS.gene83357:intron	30.0%
!!!	ATGCTATTTTCTATACGGAA+AGG	+	chr7.3:13690870-13690889	MS.gene83357:CDS	30.0%
	AAAGAGAACTAGTTACGGTT+GGG	-	chr7.3:13690270-13690289	None:intergenic	35.0%
	AACGAAATTGAAGCATATGG+AGG	-	chr7.3:13690327-13690346	None:intergenic	35.0%
	ACAAAAAGCAGGACACTTTA+CGG	+	chr7.3:13690433-13690452	MS.gene83357:CDS	35.0%
	AGACTGAATCTCTTGAAGAA+GGG	-	chr7.3:13690695-13690714	None:intergenic	35.0%
	AGAGATTCAGTCTGAGAAAA+AGG	+	chr7.3:13690702-13690721	MS.gene83357:CDS	35.0%
	CTTACTTGTGAATGTCTTGT+TGG	+	chr7.3:13690801-13690820	MS.gene83357:intron	35.0%
	GAGAGAAAGAGAACTAGTTA+CGG	-	chr7.3:13690275-13690294	None:intergenic	35.0%
	TATCCTGACATTCAACATCA+CGG	+	chr7.3:13690646-13690665	MS.gene83357:intron	35.0%
	TCGTAACTATTAGGGTTTCA+CGG	+	chr7.3:13690352-13690371	MS.gene83357:intron	35.0%
!	CTTTTCAGTTTTCTGCAAGA+AGG	-	chr7.3:13690828-13690847	None:intergenic	35.0%
!!	ATTAGAGCTAGACTTGTTGA+TGG	+	chr7.3:13690610-13690629	MS.gene83357:CDS	35.0%
	AATCTCTTGAAGAAGGGTTC+TGG	-	chr7.3:13690689-13690708	None:intergenic	40.0%
	AGCACAAAGACTATCTCCTT+CGG	+	chr7.3:13690397-13690416	MS.gene83357:CDS	40.0%
	ATCCTGACATTCAACATCAC+GGG	+	chr7.3:13690647-13690666	MS.gene83357:intron	40.0%
	CAAAAAGCAGGACACTTTAC+GGG	+	chr7.3:13690434-13690453	MS.gene83357:CDS	40.0%
	CAGACTGAATCTCTTGAAGA+AGG	-	chr7.3:13690696-13690715	None:intergenic	40.0%
	GAAAGAGAACTAGTTACGGT+TGG	-	chr7.3:13690271-13690290	None:intergenic	40.0%
	GATTCAGTCTGAGAAAAAGG+TGG	+	chr7.3:13690705-13690724	MS.gene83357:intron	40.0%
	GCTCTTTGTAAGTACGTCTA+CGG	-	chr7.3:13690244-13690263	None:intergenic	40.0%
	TCTACGGATGATGTTTCTCA+GGG	-	chr7.3:13690228-13690247	None:intergenic	40.0%
	AGGGTTTCACGGAAACGATT+TGG	+	chr7.3:13690363-13690382	MS.gene83357:intron	45.0%
	CAGTCTGAGAAAAAGGTGGA+TGG	+	chr7.3:13690709-13690728	MS.gene83357:intron	45.0%
	GCACAAAGACTATCTCCTTC+GGG	+	chr7.3:13690398-13690417	MS.gene83357:CDS	45.0%
	GCTTGTCTGTCAGACACTTA+AGG	+	chr7.3:13690543-13690562	MS.gene83357:intron	45.0%
	GTCTACGGATGATGTTTCTC+AGG	-	chr7.3:13690229-13690248	None:intergenic	45.0%
	TTCCCGTGATGTTGAATGTC+AGG	-	chr7.3:13690652-13690671	None:intergenic	45.0%
!	TGTGATAAGCTTTAGCCCGA+AGG	-	chr7.3:13690416-13690435	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	13690218	13690919	13690218	ID=MS.gene83357
chr7.3	mRNA	13690218	13690919	13690218	ID=MS.gene83357.t1;Parent=MS.gene83357
chr7.3	exon	13690218	13690273	13690218	ID=MS.gene83357.t1.exon1;Parent=MS.gene83357.t1
chr7.3	CDS	13690218	13690273	13690218	ID=cds.MS.gene83357.t1;Parent=MS.gene83357.t1
chr7.3	exon	13690365	13690455	13690365	ID=MS.gene83357.t1.exon2;Parent=MS.gene83357.t1
chr7.3	CDS	13690365	13690455	13690365	ID=cds.MS.gene83357.t1;Parent=MS.gene83357.t1
chr7.3	exon	13690556	13690644	13690556	ID=MS.gene83357.t1.exon3;Parent=MS.gene83357.t1
chr7.3	CDS	13690556	13690644	13690556	ID=cds.MS.gene83357.t1;Parent=MS.gene83357.t1
chr7.3	exon	13690690	13690723	13690690	ID=MS.gene83357.t1.exon4;Parent=MS.gene83357.t1
chr7.3	CDS	13690690	13690723	13690690	ID=cds.MS.gene83357.t1;Parent=MS.gene83357.t1
chr7.3	exon	13690836	13690919	13690836	ID=MS.gene83357.t1.exon5;Parent=MS.gene83357.t1
chr7.3	CDS	13690836	13690919	13690836	ID=cds.MS.gene83357.t1;Parent=MS.gene83357.t1

Gene Sequence

>MS.gene83357

ATGGCTTCCCTGAGAAACATCATCCGTAGACGTACTTACAAAGAGCGTGCCCAACCGTAACTAGTTCTCTTTCTCTCACAATTATCGTTGATATGCTCTGTATCTGCCTCCATATGCTTCAATTTCGTTTGTTTTCGTAACTATTAGGGTTTCACGGAAACGATTTGGAATACTCGAAAAGCACAAAGACTATCTCCTTCGGGCTAAAGCTTATCACAAAAAGCAGGACACTTTACGGGTATTTAATTTTCATTCATGCTTAGTTTCAAATTTGGTGATTTTGATGATAGTTTCTATCTTTAAATCATCGTTTTATCTATTATTTGCTTGTCTGTCAGACACTTAAGGAAAAAGCTGCAAATAGAAACCCAGATGAATTTTACTTTAAGATGATTAGAGCTAGACTTGTTGATGGAGTTCATACTAAGTATCCTGACATTCAACATCACGGGAAACATGACAATCAGACCAGAACCCTTCTTCAAGAGATTCAGTCTGAGAAAAAGGTGGATGGTTTATTTGTCTCTGTTTTTTTTTTTTTTTTTTTGTAATATTTATTGTTGCGCTGTATATGCCAAATTTGCTTACTTGTGAATGTCTTGTTGGCCCTTCTTGCAGAAAACTGAAAAGATAAACGCCGAACTGAATTCTCATGCTATTTTCTATACGGAAAGGTTTGAGTTTCTACCAGAACAAGCATGA

Protein sequence

>MS.gene83357.t1

MASLRNIIRRRTYKERAQPVSRKRFGILEKHKDYLLRAKAYHKKQDTLRTLKEKAANRNPDEFYFKMIRARLVDGVHTKTLLQEIQSEKKKTEKINAELNSHAIFYTERFEFLPEQA