Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86606.t1 | XP_024631911.1 | 92.9 | 168 | 12 | 0 | 1 | 168 | 416 | 583 | 7.40E-61 | 243.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86606.t1 | A0A072TGZ8 | 91.5 | 130 | 11 | 0 | 39 | 168 | 1 | 130 | 1.7e-38 | 168.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene86606 | 0.816545 | 5.23E-52 | -1.69E-46 |
| MS.gene049404 | MS.gene86606 | 0.812161 | 4.87E-51 | -1.69E-46 |
| MS.gene050026 | MS.gene86606 | 0.856499 | 3.09E-62 | -1.69E-46 |
| MS.gene050029 | MS.gene86606 | 0.8098 | 1.58E-50 | -1.69E-46 |
| MS.gene050355 | MS.gene86606 | 0.812535 | 4.04E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene86606 | 0.803064 | 4.17E-49 | -1.69E-46 |
| MS.gene051279 | MS.gene86606 | 0.805853 | 1.09E-49 | -1.69E-46 |
| MS.gene051594 | MS.gene86606 | 0.82173 | 3.46E-53 | -1.69E-46 |
| MS.gene051744 | MS.gene86606 | 0.805855 | 1.09E-49 | -1.69E-46 |
| MS.gene052190 | MS.gene86606 | 0.801932 | 7.13E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene86606 | 0.811329 | 7.39E-51 | -1.69E-46 |
| MS.gene052519 | MS.gene86606 | 0.841224 | 5.41E-58 | -1.69E-46 |
| MS.gene052582 | MS.gene86606 | 0.817271 | 3.60E-52 | -1.69E-46 |
| MS.gene052584 | MS.gene86606 | 0.816561 | 5.19E-52 | -1.69E-46 |
| MS.gene052773 | MS.gene86606 | 0.802308 | 5.97E-49 | -1.69E-46 |
| MS.gene052867 | MS.gene86606 | 0.827194 | 1.79E-54 | -1.69E-46 |
| MS.gene053145 | MS.gene86606 | 0.810549 | 1.09E-50 | -1.69E-46 |
| MS.gene053226 | MS.gene86606 | 0.803875 | 2.83E-49 | -1.69E-46 |
| MS.gene05414 | MS.gene86606 | 0.811969 | 5.36E-51 | -1.69E-46 |
| MS.gene05415 | MS.gene86606 | 0.802018 | 6.84E-49 | -1.69E-46 |
| MS.gene054236 | MS.gene86606 | 0.828533 | 8.53E-55 | -1.69E-46 |
| MS.gene054291 | MS.gene86606 | -0.803631 | 3.18E-49 | -1.69E-46 |
| MS.gene054651 | MS.gene86606 | 0.84082 | 6.90E-58 | -1.69E-46 |
| MS.gene054775 | MS.gene86606 | 0.808143 | 3.58E-50 | -1.69E-46 |
| MS.gene055586 | MS.gene86606 | 0.834746 | 2.51E-56 | -1.69E-46 |
| MS.gene055589 | MS.gene86606 | 0.839232 | 1.79E-57 | -1.69E-46 |
| MS.gene055818 | MS.gene86606 | 0.826238 | 3.03E-54 | -1.69E-46 |
| MS.gene056174 | MS.gene86606 | 0.811057 | 8.47E-51 | -1.69E-46 |
| MS.gene056366 | MS.gene86606 | 0.823068 | 1.69E-53 | -1.69E-46 |
| MS.gene057026 | MS.gene86606 | 0.802022 | 6.83E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene86606 | 0.827619 | 1.42E-54 | -1.69E-46 |
| MS.gene058010 | MS.gene86606 | 0.804939 | 1.70E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene86606 | 0.832836 | 7.53E-56 | -1.69E-46 |
| MS.gene058625 | MS.gene86606 | 0.814051 | 1.88E-51 | -1.69E-46 |
| MS.gene058783 | MS.gene86606 | 0.813216 | 2.86E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene86606 | 0.820594 | 6.32E-53 | -1.69E-46 |
| MS.gene05925 | MS.gene86606 | 0.803414 | 3.53E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene86606 | 0.84538 | 4.21E-59 | -1.69E-46 |
| MS.gene059584 | MS.gene86606 | 0.822967 | 1.78E-53 | -1.69E-46 |
| MS.gene059625 | MS.gene86606 | 0.8218 | 3.33E-53 | -1.69E-46 |
| MS.gene060011 | MS.gene86606 | 0.808808 | 2.58E-50 | -1.69E-46 |
| MS.gene060322 | MS.gene86606 | 0.80545 | 1.33E-49 | -1.69E-46 |
| MS.gene060521 | MS.gene86606 | 0.806274 | 8.91E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene86606.t1 | MTR_0125s0060 | 91.538 | 130 | 11 | 0 | 39 | 168 | 1 | 130 | 1.81e-80 | 234 |
| MS.gene86606.t1 | MTR_2g035110 | 81.538 | 130 | 24 | 0 | 39 | 168 | 1 | 130 | 2.53e-68 | 204 |
| MS.gene86606.t1 | MTR_8g066845 | 58.416 | 101 | 22 | 3 | 69 | 168 | 1 | 82 | 5.30e-24 | 90.1 |
| MS.gene86606.t1 | MTR_4g012560 | 72.581 | 62 | 12 | 1 | 86 | 147 | 24 | 80 | 1.90e-18 | 75.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 35 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCCATCTGGTTTCCCTTT+TGG | 0.170423 | 6.1:+50719304 | None:intergenic |
| AATTGACAATGATATTTATA+TGG | 0.247121 | 6.1:-50719099 | MS.gene86606:CDS |
| AACAAGAAGGGGCGCATATT+TGG | 0.259894 | 6.1:-50719059 | MS.gene86606:CDS |
| TGAATTGAAGTGTTCATATT+AGG | 0.299166 | 6.1:+50718834 | None:intergenic |
| CCATTATCACCTTCTCCTTC+CGG | 0.338322 | 6.1:+50718810 | None:intergenic |
| TTCTACCGATGTAGATTCTT+CGG | 0.370531 | 6.1:-50718982 | MS.gene86606:CDS |
| AAGGGGCGCATATTTGGATT+AGG | 0.373630 | 6.1:-50719053 | MS.gene86606:CDS |
| GATATTTATATGGAAATTGT+TGG | 0.391812 | 6.1:-50719089 | MS.gene86606:CDS |
| GTTTCTCAAACGGTTGAGTT+TGG | 0.394476 | 6.1:+50719165 | None:intergenic |
| TTAGAGAGTGGAATTGGATA+GGG | 0.406611 | 6.1:-50719365 | MS.gene86606:intron |
| GAGCGCTTGGAGCAAATGAT+TGG | 0.408396 | 6.1:-50718867 | MS.gene86606:CDS |
| TTTAGAGAGTGGAATTGGAT+AGG | 0.411311 | 6.1:-50719366 | MS.gene86606:intron |
| GACAAACTTCATAGACCAAA+AGG | 0.414225 | 6.1:-50719319 | MS.gene86606:CDS |
| CCGGAAGGAGAAGGTGATAA+TGG | 0.416904 | 6.1:-50718810 | MS.gene86606:CDS |
| CTTTGAGAGTGTTTCTCAAA+CGG | 0.455557 | 6.1:+50719155 | None:intergenic |
| GTTTCATTAAACCACCCATC+TGG | 0.467331 | 6.1:+50719292 | None:intergenic |
| AGAAACGCTAAAACTGAAGA+TGG | 0.499466 | 6.1:-50718889 | MS.gene86606:CDS |
| AAAACTGAAGATGGAGCGCT+TGG | 0.500382 | 6.1:-50718880 | MS.gene86606:CDS |
| TGTTGGAGACATTAACAAGA+AGG | 0.504551 | 6.1:-50719072 | MS.gene86606:CDS |
| TCATTGTCAATTTCTTCAGT+AGG | 0.525205 | 6.1:+50719110 | None:intergenic |
| AGCGCTTGGAGCAAATGATT+GGG | 0.526657 | 6.1:-50718866 | MS.gene86606:CDS |
| TATGAACACTTCAATTCAGC+CGG | 0.533653 | 6.1:-50718829 | MS.gene86606:CDS |
| GTTGGAGACATTAACAAGAA+GGG | 0.548845 | 6.1:-50719071 | MS.gene86606:CDS |
| TCAATTCAGCCGGAAGGAGA+AGG | 0.553755 | 6.1:-50718819 | MS.gene86606:CDS |
| TCTACATCGGTAGAAATTGA+AGG | 0.554924 | 6.1:+50718990 | None:intergenic |
| ACAAACTTCATAGACCAAAA+GGG | 0.556595 | 6.1:-50719318 | MS.gene86606:CDS |
| AGACCAAAAGGGAAACCAGA+TGG | 0.568337 | 6.1:-50719307 | MS.gene86606:CDS |
| GACCAAAAGGGAAACCAGAT+GGG | 0.568542 | 6.1:-50719306 | MS.gene86606:CDS |
| TTGGAGACATTAACAAGAAG+GGG | 0.585345 | 6.1:-50719070 | MS.gene86606:CDS |
| TCGGTAGAAATTGAAGGCGA+TGG | 0.604104 | 6.1:+50718996 | None:intergenic |
| TCGTAAATCTCAAACCATGT+TGG | 0.611440 | 6.1:+50719340 | None:intergenic |
| AACACTTCAATTCAGCCGGA+AGG | 0.621409 | 6.1:-50718825 | MS.gene86606:CDS |
| AATTGGATAGGGAGCCAACA+TGG | 0.624450 | 6.1:-50719354 | MS.gene86606:CDS |
| TTTGAGAAACACTCTCAAAG+TGG | 0.633906 | 6.1:-50719152 | MS.gene86606:CDS |
| CAAAAGGGAAACCAGATGGG+TGG | 0.669221 | 6.1:-50719303 | MS.gene86606:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGACAATGATATTTATA+TGG | - | chr6.1:50719140-50719159 | MS.gene86606:CDS | 15.0% |
| !! | GATATTTATATGGAAATTGT+TGG | - | chr6.1:50719150-50719169 | MS.gene86606:CDS | 20.0% |
| !! | TGAATTGAAGTGTTCATATT+AGG | + | chr6.1:50719408-50719427 | None:intergenic | 25.0% |
| !!! | CATACTAATTTGTTGTTTGT+TGG | - | chr6.1:50718844-50718863 | MS.gene86606:CDS | 25.0% |
| !!! | TAATAGTAACTCTTTTTTGC+AGG | - | chr6.1:50719044-50719063 | MS.gene86606:CDS | 25.0% |
| ACAAACTTCATAGACCAAAA+GGG | - | chr6.1:50718921-50718940 | MS.gene86606:CDS | 30.0% | |
| TCATTGTCAATTTCTTCAGT+AGG | + | chr6.1:50719132-50719151 | None:intergenic | 30.0% | |
| ! | TTTTTCCGAAGAATCTACAT+CGG | + | chr6.1:50719265-50719284 | None:intergenic | 30.0% |
| !!! | TATGTTTTAGAGAGTGGAAT+TGG | - | chr6.1:50718868-50718887 | MS.gene86606:CDS | 30.0% |
| AGAAACGCTAAAACTGAAGA+TGG | - | chr6.1:50719350-50719369 | MS.gene86606:CDS | 35.0% | |
| GACAAACTTCATAGACCAAA+AGG | - | chr6.1:50718920-50718939 | MS.gene86606:CDS | 35.0% | |
| GTTGGAGACATTAACAAGAA+GGG | - | chr6.1:50719168-50719187 | MS.gene86606:CDS | 35.0% | |
| TATGAACACTTCAATTCAGC+CGG | - | chr6.1:50719410-50719429 | MS.gene86606:intron | 35.0% | |
| TCGTAAATCTCAAACCATGT+TGG | + | chr6.1:50718902-50718921 | None:intergenic | 35.0% | |
| TCTACATCGGTAGAAATTGA+AGG | + | chr6.1:50719252-50719271 | None:intergenic | 35.0% | |
| TGTTGGAGACATTAACAAGA+AGG | - | chr6.1:50719167-50719186 | MS.gene86606:CDS | 35.0% | |
| TTAGAGAGTGGAATTGGATA+GGG | - | chr6.1:50718874-50718893 | MS.gene86606:CDS | 35.0% | |
| TTCTACCGATGTAGATTCTT+CGG | - | chr6.1:50719257-50719276 | MS.gene86606:intron | 35.0% | |
| TTGGAGACATTAACAAGAAG+GGG | - | chr6.1:50719169-50719188 | MS.gene86606:CDS | 35.0% | |
| TTTAGAGAGTGGAATTGGAT+AGG | - | chr6.1:50718873-50718892 | MS.gene86606:CDS | 35.0% | |
| TTTGAGAAACACTCTCAAAG+TGG | - | chr6.1:50719087-50719106 | MS.gene86606:CDS | 35.0% | |
| !! | CTTTGAGAGTGTTTCTCAAA+CGG | + | chr6.1:50719087-50719106 | None:intergenic | 35.0% |
| !!! | GTTGGTTATGTTTTAGAGAG+TGG | - | chr6.1:50718862-50718881 | MS.gene86606:CDS | 35.0% |
| GTTTCATTAAACCACCCATC+TGG | + | chr6.1:50718950-50718969 | None:intergenic | 40.0% | |
| GTTTCTCAAACGGTTGAGTT+TGG | + | chr6.1:50719077-50719096 | None:intergenic | 40.0% | |
| TTCGGAAAAAGTTGCTGCTA+TGG | - | chr6.1:50719275-50719294 | MS.gene86606:intron | 40.0% | |
| AACACTTCAATTCAGCCGGA+AGG | - | chr6.1:50719414-50719433 | MS.gene86606:intron | 45.0% | |
| AATTGGATAGGGAGCCAACA+TGG | - | chr6.1:50718885-50718904 | MS.gene86606:CDS | 45.0% | |
| AGACCAAAAGGGAAACCAGA+TGG | - | chr6.1:50718932-50718951 | MS.gene86606:CDS | 45.0% | |
| AGCGCTTGGAGCAAATGATT+GGG | - | chr6.1:50719373-50719392 | MS.gene86606:intron | 45.0% | |
| CCATTATCACCTTCTCCTTC+CGG | + | chr6.1:50719432-50719451 | None:intergenic | 45.0% | |
| GACCAAAAGGGAAACCAGAT+GGG | - | chr6.1:50718933-50718952 | MS.gene86606:CDS | 45.0% | |
| GGAAAAAGTTGCTGCTATGG+AGG | - | chr6.1:50719278-50719297 | MS.gene86606:CDS | 45.0% | |
| TCGGTAGAAATTGAAGGCGA+TGG | + | chr6.1:50719246-50719265 | None:intergenic | 45.0% | |
| ! | AAAACTGAAGATGGAGCGCT+TGG | - | chr6.1:50719359-50719378 | MS.gene86606:CDS | 45.0% |
| ! | AACAAGAAGGGGCGCATATT+TGG | - | chr6.1:50719180-50719199 | MS.gene86606:intron | 45.0% |
| ! | AAGGGGCGCATATTTGGATT+AGG | - | chr6.1:50719186-50719205 | MS.gene86606:intron | 45.0% |
| CAAAAGGGAAACCAGATGGG+TGG | - | chr6.1:50718936-50718955 | MS.gene86606:CDS | 50.0% | |
| CCGGAAGGAGAAGGTGATAA+TGG | - | chr6.1:50719429-50719448 | MS.gene86606:intron | 50.0% | |
| GAGCGCTTGGAGCAAATGAT+TGG | - | chr6.1:50719372-50719391 | MS.gene86606:intron | 50.0% | |
| TCAATTCAGCCGGAAGGAGA+AGG | - | chr6.1:50719420-50719439 | MS.gene86606:intron | 50.0% | |
| !! | CACCCATCTGGTTTCCCTTT+TGG | + | chr6.1:50718938-50718957 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 50718800 | 50719461 | 50718800 | ID=MS.gene86606 |
| chr6.1 | mRNA | 50718800 | 50719461 | 50718800 | ID=MS.gene86606.t1;Parent=MS.gene86606 |
| chr6.1 | exon | 50719459 | 50719461 | 50719459 | ID=MS.gene86606.t1.exon1;Parent=MS.gene86606.t1 |
| chr6.1 | CDS | 50719459 | 50719461 | 50719459 | ID=cds.MS.gene86606.t1;Parent=MS.gene86606.t1 |
| chr6.1 | exon | 50719276 | 50719383 | 50719276 | ID=MS.gene86606.t1.exon2;Parent=MS.gene86606.t1 |
| chr6.1 | CDS | 50719276 | 50719383 | 50719276 | ID=cds.MS.gene86606.t1;Parent=MS.gene86606.t1 |
| chr6.1 | exon | 50718800 | 50719195 | 50718800 | ID=MS.gene86606.t1.exon3;Parent=MS.gene86606.t1 |
| chr6.1 | CDS | 50718800 | 50719195 | 50718800 | ID=cds.MS.gene86606.t1;Parent=MS.gene86606.t1 |
| Gene Sequence |
| Protein sequence |
