AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene88781


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88781.t1 MTR_5g070210 88.667 150 17 0 2 151 14 163 6.35e-97 282
MS.gene88781.t1 MTR_5g070230 62.602 123 46 0 31 153 10 132 6.62e-53 166
MS.gene88781.t1 MTR_5g070250 59.677 62 24 1 94 154 20 81 6.12e-20 83.2
MS.gene88781.t1 MTR_8g073540 46.341 82 44 0 61 142 28 109 6.07e-18 79.3
MS.gene88781.t1 MTR_1g095500 45.122 82 45 0 61 142 28 109 9.14e-18 79.0
MS.gene88781.t1 MTR_5g070270 50.000 98 34 6 10 96 23 116 1.33e-17 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene88781.t1 AT5G58770 65.217 92 32 0 63 154 74 165 6.97e-41 139
MS.gene88781.t1 AT5G58782 53.000 100 47 0 55 154 46 145 2.66e-30 111
MS.gene88781.t1 AT5G58782 53.000 100 47 0 55 154 64 163 3.20e-30 111
MS.gene88781.t1 AT5G58780 55.914 93 41 0 59 151 63 155 5.12e-29 108
MS.gene88781.t1 AT2G23400 41.085 129 75 1 26 154 31 158 1.14e-28 105
MS.gene88781.t1 AT2G23400 41.085 129 75 1 26 154 31 158 1.61e-27 104
MS.gene88781.t1 AT2G23410 49.474 95 47 1 61 154 65 159 8.29e-25 97.4
MS.gene88781.t1 AT5G58784 48.889 90 46 0 65 154 69 158 1.50e-24 96.7
MS.gene88781.t1 AT2G17570 47.561 82 43 0 66 147 39 120 7.75e-19 81.3

Find 46 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCATTCTCAACCGATAAT+TGG 0.274745 5.4:-55086774 MS.gene88781:CDS
TAAGCATGTGGCGGTGATTA+TGG 0.306230 5.4:-55086923 MS.gene88781:CDS
GTGTTATAGTTGGGGAATAA+AGG 0.324367 5.4:-55086809 MS.gene88781:CDS
CTCATTCTCGATATTCTTCA+AGG 0.331892 5.4:-55087071 MS.gene88781:CDS
ATGGGAGGTGGGCAAAGATG+AGG 0.382978 5.4:-55086894 MS.gene88781:CDS
GGTGAGGTTGTGTTATAGTT+GGG 0.400487 5.4:-55086818 MS.gene88781:CDS
TACCACTAACCACCCTCATC+GGG 0.419799 5.4:-55087112 MS.gene88781:CDS
CTTAGGTCGAATCCAATTAT+CGG 0.425110 5.4:+55086762 None:intergenic
TGGTGAGGTTGTGTTATAGT+TGG 0.451686 5.4:-55086819 MS.gene88781:CDS
CGGTGATTATGGACGGGAAT+GGG 0.455731 5.4:-55086912 MS.gene88781:CDS
TGGGAGGTGGGCAAAGATGA+GGG 0.455809 5.4:-55086893 MS.gene88781:CDS
GGGAGGTGGGCAAAGATGAG+GGG 0.456021 5.4:-55086892 MS.gene88781:CDS
GGAGGTGGGCAAAGATGAGG+GGG 0.479875 5.4:-55086891 MS.gene88781:CDS
CGATAATTGGATTCGACCTA+AGG 0.480618 5.4:-55086761 MS.gene88781:intron
CCGTCTGCGGGTCATGTAGC+CGG 0.489572 5.4:-55086862 MS.gene88781:CDS
TTATGGACGGGAATGGGAGG+TGG 0.493196 5.4:-55086906 MS.gene88781:CDS
CACGGCAATAGTGACGCGTT+TGG 0.496192 5.4:+55087041 None:intergenic
AGCTCCGCTTGCAATTCCTC+TGG 0.511293 5.4:+55086952 None:intergenic
GCGGTGATTATGGACGGGAA+TGG 0.517607 5.4:-55086913 MS.gene88781:CDS
CTACCACTAACCACCCTCAT+CGG 0.530891 5.4:-55087113 MS.gene88781:CDS
CGGCTACATGACCCGCAGAC+GGG 0.532986 5.4:+55086863 None:intergenic
AATTTATTGTTCTTTCAAAG+AGG 0.535873 5.4:+55086605 None:intergenic
GCTTGCAATTCCTCTGGCAA+TGG 0.544403 5.4:+55086958 None:intergenic
GTGAGGTTGTGTTATAGTTG+GGG 0.555675 5.4:-55086817 MS.gene88781:CDS
ATAATCACCGCCACATGCTT+AGG 0.560502 5.4:+55086925 None:intergenic
TATGGACGGGAATGGGAGGT+GGG 0.565954 5.4:-55086905 MS.gene88781:CDS
ATGTGGCGGTGATTATGGAC+GGG 0.576395 5.4:-55086918 MS.gene88781:CDS
TGTAAAGTCGTTGAGAAGGA+TGG 0.579930 5.4:-55086839 MS.gene88781:CDS
GGCTACATGACCCGCAGACG+GGG 0.586275 5.4:+55086864 None:intergenic
CATGTGGCGGTGATTATGGA+CGG 0.587809 5.4:-55086919 MS.gene88781:CDS
CTGAAATTGAAGCACTAGAG+AGG 0.594486 5.4:-55086582 MS.gene88781:CDS
CAAACGCGTCACTATTGCCG+TGG 0.602876 5.4:-55087040 MS.gene88781:CDS
TGATTATGGACGGGAATGGG+AGG 0.615382 5.4:-55086909 MS.gene88781:CDS
CACCTGATCCTGAAAAGCCA+CGG 0.615415 5.4:+55087023 None:intergenic
CCGGCTACATGACCCGCAGA+CGG 0.616650 5.4:+55086862 None:intergenic
CCGGTGTAAAGTCGTTGAGA+AGG 0.624155 5.4:-55086843 MS.gene88781:CDS
AGTCGTTGAGAAGGATGGTG+AGG 0.625194 5.4:-55086834 MS.gene88781:CDS
GGAGCTGATGCCTAAGCATG+TGG 0.626089 5.4:-55086935 MS.gene88781:CDS
AGAAATGGAGCCATTGCCAG+AGG 0.635367 5.4:-55086968 MS.gene88781:CDS
GGTGGATGGGGAAACTTCCG+TGG 0.636917 5.4:-55087004 MS.gene88781:CDS
CCTTCTCAACGACTTTACAC+CGG 0.638232 5.4:+55086843 None:intergenic
CCACTAACCACCCTCATCGG+GGG 0.665764 5.4:-55087110 MS.gene88781:CDS
ATTGCCAGAGGAATTGCAAG+CGG 0.666528 5.4:-55086956 MS.gene88781:CDS
GCTACATGACCCGCAGACGG+GGG 0.669075 5.4:+55086865 None:intergenic
ACCACTAACCACCCTCATCG+GGG 0.670600 5.4:-55087111 MS.gene88781:CDS
GCTGATGCCTAAGCATGTGG+CGG 0.712093 5.4:-55086932 MS.gene88781:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTCTTAGTTTTGATTAT+TGG - chr5.4:55087005-55087024 MS.gene88781:CDS 15.0%
!! AATTTATTGTTCTTTCAAAG+AGG + chr5.4:55087073-55087092 None:intergenic 20.0%
! AGTGAAATTAAAATGACCTT+AGG + chr5.4:55086933-55086952 None:intergenic 25.0%
! ATCACATTAGTTATTGATAG+TGG - chr5.4:55086982-55087001 MS.gene88781:CDS 25.0%
!!! GTTTTGATTATTGGTGTTAC+TGG - chr5.4:55087014-55087033 MS.gene88781:CDS 30.0%
CTCATTCTCGATATTCTTCA+AGG - chr5.4:55086604-55086623 MS.gene88781:CDS 35.0%
CTTAGGTCGAATCCAATTAT+CGG + chr5.4:55086916-55086935 None:intergenic 35.0%
GTGTTATAGTTGGGGAATAA+AGG - chr5.4:55086866-55086885 MS.gene88781:CDS 35.0%
TTGCATTCTCAACCGATAAT+TGG - chr5.4:55086901-55086920 MS.gene88781:CDS 35.0%
! TTCTGAAAAATCTTCGTCCA+CGG + chr5.4:55086691-55086710 None:intergenic 35.0%
!! GGACGAAGATTTTTCAGAAA+TGG - chr5.4:55086692-55086711 MS.gene88781:intron 35.0%
CGATAATTGGATTCGACCTA+AGG - chr5.4:55086914-55086933 MS.gene88781:CDS 40.0%
GGTGAGGTTGTGTTATAGTT+GGG - chr5.4:55086857-55086876 MS.gene88781:CDS 40.0%
GTGAGGTTGTGTTATAGTTG+GGG - chr5.4:55086858-55086877 MS.gene88781:CDS 40.0%
TGGTGAGGTTGTGTTATAGT+TGG - chr5.4:55086856-55086875 MS.gene88781:CDS 40.0%
TGTAAAGTCGTTGAGAAGGA+TGG - chr5.4:55086836-55086855 MS.gene88781:CDS 40.0%
! CTGAAATTGAAGCACTAGAG+AGG - chr5.4:55087093-55087112 MS.gene88781:CDS 40.0%
ATAATCACCGCCACATGCTT+AGG + chr5.4:55086753-55086772 None:intergenic 45.0%
ATTGCCAGAGGAATTGCAAG+CGG - chr5.4:55086719-55086738 MS.gene88781:intron 45.0%
CCTTCTCAACGACTTTACAC+CGG + chr5.4:55086835-55086854 None:intergenic 45.0%
TAAGCATGTGGCGGTGATTA+TGG - chr5.4:55086752-55086771 MS.gene88781:intron 45.0%
TGTTACTGGTGATATGCAGG+TGG - chr5.4:55087028-55087047 MS.gene88781:CDS 45.0%
!! CTTTTCAGGATCAGGTGGAT+GGG - chr5.4:55086658-55086677 MS.gene88781:intron 45.0%
!! TGGTGTTACTGGTGATATGC+AGG - chr5.4:55087025-55087044 MS.gene88781:CDS 45.0%
!! TTTTCAGGATCAGGTGGATG+GGG - chr5.4:55086659-55086678 MS.gene88781:intron 45.0%
AGAAATGGAGCCATTGCCAG+AGG - chr5.4:55086707-55086726 MS.gene88781:intron 50.0%
AGTCGTTGAGAAGGATGGTG+AGG - chr5.4:55086841-55086860 MS.gene88781:CDS 50.0%
ATGTGGCGGTGATTATGGAC+GGG - chr5.4:55086757-55086776 MS.gene88781:intron 50.0%
CACCTGATCCTGAAAAGCCA+CGG + chr5.4:55086655-55086674 None:intergenic 50.0%
CATGTGGCGGTGATTATGGA+CGG - chr5.4:55086756-55086775 MS.gene88781:intron 50.0%
CCGGTGTAAAGTCGTTGAGA+AGG - chr5.4:55086832-55086851 MS.gene88781:CDS 50.0%
CGGTGATTATGGACGGGAAT+GGG - chr5.4:55086763-55086782 MS.gene88781:CDS 50.0%
CTACCACTAACCACCCTCAT+CGG - chr5.4:55086562-55086581 MS.gene88781:CDS 50.0%
GCTTGCAATTCCTCTGGCAA+TGG + chr5.4:55086720-55086739 None:intergenic 50.0%
TACCACTAACCACCCTCATC+GGG - chr5.4:55086563-55086582 MS.gene88781:CDS 50.0%
TGATTATGGACGGGAATGGG+AGG - chr5.4:55086766-55086785 MS.gene88781:CDS 50.0%
! CACTATTGCCGTGGCTTTTC+AGG - chr5.4:55086644-55086663 MS.gene88781:intron 50.0%
!! GCTTTTCAGGATCAGGTGGA+TGG - chr5.4:55086657-55086676 MS.gene88781:intron 50.0%
ACCACTAACCACCCTCATCG+GGG - chr5.4:55086564-55086583 MS.gene88781:CDS 55.0%
AGCTCCGCTTGCAATTCCTC+TGG + chr5.4:55086726-55086745 None:intergenic 55.0%
ATCTGACTGATCCCCCGATG+AGG + chr5.4:55086579-55086598 None:intergenic 55.0%
ATGGGAGGTGGGCAAAGATG+AGG - chr5.4:55086781-55086800 MS.gene88781:CDS 55.0%
CAAACGCGTCACTATTGCCG+TGG - chr5.4:55086635-55086654 MS.gene88781:intron 55.0%
CACGGCAATAGTGACGCGTT+TGG + chr5.4:55086637-55086656 None:intergenic 55.0%
GCGGTGATTATGGACGGGAA+TGG - chr5.4:55086762-55086781 MS.gene88781:CDS 55.0%
GCTGATGCCTAAGCATGTGG+CGG - chr5.4:55086743-55086762 MS.gene88781:intron 55.0%
GGAGCTGATGCCTAAGCATG+TGG - chr5.4:55086740-55086759 MS.gene88781:intron 55.0%
TATGGACGGGAATGGGAGGT+GGG - chr5.4:55086770-55086789 MS.gene88781:CDS 55.0%
TCTGACTGATCCCCCGATGA+GGG + chr5.4:55086578-55086597 None:intergenic 55.0%
TGGGAGGTGGGCAAAGATGA+GGG - chr5.4:55086782-55086801 MS.gene88781:CDS 55.0%
TTATGGACGGGAATGGGAGG+TGG - chr5.4:55086769-55086788 MS.gene88781:CDS 55.0%
!! CGTGGCTTTTCAGGATCAGG+TGG - chr5.4:55086653-55086672 MS.gene88781:intron 55.0%
!! TGCCGTGGCTTTTCAGGATC+AGG - chr5.4:55086650-55086669 MS.gene88781:intron 55.0%
CCACTAACCACCCTCATCGG+GGG - chr5.4:55086565-55086584 MS.gene88781:CDS 60.0%
GGAGGTGGGCAAAGATGAGG+GGG - chr5.4:55086784-55086803 MS.gene88781:CDS 60.0%
GGGAGGTGGGCAAAGATGAG+GGG - chr5.4:55086783-55086802 MS.gene88781:CDS 60.0%
GGTGGATGGGGAAACTTCCG+TGG - chr5.4:55086671-55086690 MS.gene88781:intron 60.0%
CCCCCGATGAGGGTGGTTAG+TGG + chr5.4:55086568-55086587 None:intergenic 65.0%
CCGGCTACATGACCCGCAGA+CGG + chr5.4:55086816-55086835 None:intergenic 65.0%
CCGTCTGCGGGTCATGTAGC+CGG - chr5.4:55086813-55086832 MS.gene88781:CDS 65.0%
CGGCTACATGACCCGCAGAC+GGG + chr5.4:55086815-55086834 None:intergenic 65.0%
GACTGATCCCCCGATGAGGG+TGG + chr5.4:55086575-55086594 None:intergenic 65.0%
GCTACATGACCCGCAGACGG+GGG + chr5.4:55086813-55086832 None:intergenic 65.0%
GGCTACATGACCCGCAGACG+GGG + chr5.4:55086814-55086833 None:intergenic 65.0%
AGGGGGTTACCCCCGTCTGC+GGG - chr5.4:55086801-55086820 MS.gene88781:CDS 70.0%
GAGGGGGTTACCCCCGTCTG+CGG - chr5.4:55086800-55086819 MS.gene88781:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 55086552 55087145 55086552 ID=MS.gene88781
chr5.4 mRNA 55086552 55087145 55086552 ID=MS.gene88781.t1;Parent=MS.gene88781
chr5.4 exon 55086762 55087145 55086762 ID=MS.gene88781.t1.exon1;Parent=MS.gene88781.t1
chr5.4 CDS 55086762 55087145 55086762 ID=cds.MS.gene88781.t1;Parent=MS.gene88781.t1
chr5.4 exon 55086552 55086650 55086552 ID=MS.gene88781.t1.exon2;Parent=MS.gene88781.t1
chr5.4 CDS 55086552 55086650 55086552 ID=cds.MS.gene88781.t1;Parent=MS.gene88781.t1
Gene Sequence

>MS.gene88781

ATGACTACTACTACCACTAACCACCCTCATCGGGGGATCAGTCAGATACAAACTCATTCTCGATATTCTTCAAGGTTTGTTACCAAACGCGTCACTATTGCCGTGGCTTTTCAGGATCAGGTGGATGGGGAAACTTCCGTGGACGAAGATTTTTCAGAAATGGAGCCATTGCCAGAGGAATTGCAAGCGGAGCTGATGCCTAAGCATGTGGCGGTGATTATGGACGGGAATGGGAGGTGGGCAAAGATGAGGGGGTTACCCCCGTCTGCGGGTCATGTAGCCGGTGTAAAGTCGTTGAGAAGGATGGTGAGGTTGTGTTATAGTTGGGGAATAAAGGTTCTAACTGTTTTTGCATTCTCAACCGATAATTGGATTCGACCTAAGGTCATTTTAATTTCACTTGTTAGTTTAGTTAACTTTGATTAACATGATCACATTAGTTATTGATAGTGGTTATTCTTAGTTTTGATTATTGGTGTTACTGGTGATATGCAGGTGGAAGTTGAATTTTTGATGAGCCTCTTTGAAAGAACAATAAATTCTGAAATTGAAGCACTAGAGAGGTGTCAAATTCTAACTGCTTTAACTAATTAA

Protein sequence

>MS.gene88781.t1

MTTTTTNHPHRGISQIQTHSRYSSRFVTKRVTIAVAFQDQVDGETSVDEDFSEMEPLPEELQAELMPKHVAVIMDGNGRWAKMRGLPPSAGHVAGVKSLRRMVRLCYSWGIKVLTVFAFSTDNWIRPKVEVEFLMSLFERTINSEIEALERCQILTALTN