Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88860.t1 | KEH43619.1 | 100 | 41 | 0 | 0 | 34 | 74 | 124 | 164 | 4.20E-14 | 87 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88860.t1 | A0A072VPM9 | 100.0 | 41 | 0 | 0 | 34 | 74 | 168 | 208 | 3.0e-14 | 87.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050060 | MS.gene88860 | 0.836348 | 9.86E-57 | -1.69E-46 |
| MS.gene050426 | MS.gene88860 | -0.843018 | 1.81E-58 | -1.69E-46 |
| MS.gene050670 | MS.gene88860 | 0.813642 | 2.31E-51 | -1.69E-46 |
| MS.gene051525 | MS.gene88860 | 0.834271 | 3.30E-56 | -1.69E-46 |
| MS.gene051582 | MS.gene88860 | 0.803717 | 3.05E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene88860 | 0.830617 | 2.65E-55 | -1.69E-46 |
| MS.gene052011 | MS.gene88860 | -0.839763 | 1.30E-57 | -1.69E-46 |
| MS.gene052077 | MS.gene88860 | 0.82035 | 7.19E-53 | -1.69E-46 |
| MS.gene052188 | MS.gene88860 | 0.809925 | 1.49E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene88860 | 0.816912 | 4.33E-52 | -1.69E-46 |
| MS.gene052340 | MS.gene88860 | 0.817364 | 3.43E-52 | -1.69E-46 |
| MS.gene052469 | MS.gene88860 | 0.825557 | 4.39E-54 | -1.69E-46 |
| MS.gene054183 | MS.gene88860 | 0.828669 | 7.90E-55 | -1.69E-46 |
| MS.gene054349 | MS.gene88860 | 0.812219 | 4.73E-51 | -1.69E-46 |
| MS.gene054556 | MS.gene88860 | 0.818788 | 1.63E-52 | -1.69E-46 |
| MS.gene054719 | MS.gene88860 | 0.806119 | 9.60E-50 | -1.69E-46 |
| MS.gene055369 | MS.gene88860 | 0.803004 | 4.29E-49 | -1.69E-46 |
| MS.gene055539 | MS.gene88860 | 0.820575 | 6.38E-53 | -1.69E-46 |
| MS.gene05553 | MS.gene88860 | 0.872288 | 3.57E-67 | -1.69E-46 |
| MS.gene05556 | MS.gene88860 | 0.833785 | 4.37E-56 | -1.69E-46 |
| MS.gene056233 | MS.gene88860 | 0.819804 | 9.58E-53 | -1.69E-46 |
| MS.gene056723 | MS.gene88860 | 0.817941 | 2.54E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene88860 | -0.801997 | 6.91E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene88860 | 0.834269 | 3.31E-56 | -1.69E-46 |
| MS.gene057124 | MS.gene88860 | 0.803425 | 3.51E-49 | -1.69E-46 |
| MS.gene057396 | MS.gene88860 | 0.846283 | 2.39E-59 | -1.69E-46 |
| MS.gene058142 | MS.gene88860 | 0.828473 | 8.82E-55 | -1.69E-46 |
| MS.gene058276 | MS.gene88860 | 0.808192 | 3.49E-50 | -1.69E-46 |
| MS.gene058700 | MS.gene88860 | 0.80327 | 3.78E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene88860 | 0.808731 | 2.68E-50 | -1.69E-46 |
| MS.gene059247 | MS.gene88860 | 0.833099 | 6.48E-56 | -1.69E-46 |
| MS.gene059420 | MS.gene88860 | 0.837447 | 5.17E-57 | -1.69E-46 |
| MS.gene059868 | MS.gene88860 | 0.804943 | 1.69E-49 | -1.69E-46 |
| MS.gene059946 | MS.gene88860 | 0.811445 | 6.98E-51 | -1.69E-46 |
| MS.gene061159 | MS.gene88860 | 0.845856 | 3.13E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88860.t1 | MTR_1g097430 | 91.489 | 47 | 2 | 1 | 34 | 78 | 124 | 170 | 4.98e-23 | 87.4 |
| MS.gene88860.t1 | MTR_1g097430 | 91.489 | 47 | 2 | 1 | 34 | 78 | 168 | 214 | 8.54e-23 | 87.8 |
| MS.gene88860.t1 | MTR_1g097430 | 91.489 | 47 | 2 | 1 | 34 | 78 | 177 | 223 | 8.71e-23 | 87.8 |
| MS.gene88860.t1 | MTR_1g097430 | 91.489 | 47 | 2 | 1 | 34 | 78 | 168 | 214 | 1.32e-22 | 87.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene88860.t1 | AT1G22750 | 52.083 | 48 | 22 | 1 | 29 | 75 | 40 | 87 | 6.26e-11 | 56.2 |
| MS.gene88860.t1 | AT1G22750 | 52.083 | 48 | 22 | 1 | 29 | 75 | 40 | 87 | 6.39e-11 | 56.2 |
| MS.gene88860.t1 | AT1G22750 | 52.083 | 48 | 22 | 1 | 29 | 75 | 40 | 87 | 6.52e-11 | 56.2 |
| MS.gene88860.t1 | AT1G22750 | 53.191 | 47 | 21 | 1 | 29 | 74 | 40 | 86 | 7.31e-11 | 56.2 |
Find 10 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATCCACCTGTAAACATTT+TGG | 0.203833 | 1.1:-70134210 | None:intergenic |
| GTTGGATTTATGCGGTCTTT+TGG | 0.248345 | 1.1:+70134730 | MS.gene88860:CDS |
| GTGATTCTATTGATATGTTT+TGG | 0.353164 | 1.1:+70134753 | MS.gene88860:CDS |
| ACAAGCTCCAAAATGTTTAC+AGG | 0.395736 | 1.1:+70134203 | MS.gene88860:CDS |
| TTGCTTACAAAGAATCTAGT+TGG | 0.411156 | 1.1:+70134712 | MS.gene88860:CDS |
| TTATGCACAGCTTCTGCTTG+TGG | 0.529561 | 1.1:+70134153 | MS.gene88860:CDS |
| CCAAAATGTTTACAGGTGGA+TGG | 0.546199 | 1.1:+70134210 | MS.gene88860:CDS |
| AGCTCCAAAATGTTTACAGG+TGG | 0.614465 | 1.1:+70134206 | MS.gene88860:CDS |
| AGAATCTAGTTGGATTTATG+CGG | 0.623672 | 1.1:+70134722 | MS.gene88860:CDS |
| ATATTCCAGAGGATGCATGA+AGG | 0.678310 | 1.1:+70135036 | MS.gene88860:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTAGATAATTCTATTTCA+TGG | + | chr1.1:70133482-70133501 | MS.gene88860:intron | 15.0% |
| !! | TAAACAAACATAAAATAGTA+TGG | - | chr1.1:70134788-70134807 | None:intergenic | 15.0% |
| !! | TAATGTTCCATAAATATATT+TGG | + | chr1.1:70133440-70133459 | MS.gene88860:intron | 15.0% |
| !!! | ACTATTTTATGTTTGTTTAT+TGG | + | chr1.1:70134789-70134808 | MS.gene88860:intron | 15.0% |
| !!! | TACATTTTCTTTTTGTTAAA+AGG | + | chr1.1:70133776-70133795 | MS.gene88860:intron | 15.0% |
| !!! | TTAAATTTATTTCATTGACT+TGG | + | chr1.1:70134348-70134367 | MS.gene88860:intron | 15.0% |
| !! | AAAAAAATAAGCACCATTAT+AGG | - | chr1.1:70135014-70135033 | None:intergenic | 20.0% |
| !! | AAAAAATAAGCACCATTATA+GGG | - | chr1.1:70135013-70135032 | None:intergenic | 20.0% |
| !! | ACATTAAACATAAAATCATG+TGG | - | chr1.1:70133525-70133544 | None:intergenic | 20.0% |
| !! | CATAAATATATTTGGAGAAA+AGG | + | chr1.1:70133448-70133467 | MS.gene88860:intron | 20.0% |
| !!! | AAAAAGTATTTGTGAATTCA+AGG | + | chr1.1:70134580-70134599 | MS.gene88860:intron | 20.0% |
| !!! | ATTATATTGAGATCTTTTAG+TGG | - | chr1.1:70134559-70134578 | None:intergenic | 20.0% |
| !!! | CTTTTCTCCAAATATATTTA+TGG | - | chr1.1:70133450-70133469 | None:intergenic | 20.0% |
| !!! | TAATGTGATGTTTTCTATAT+AGG | + | chr1.1:70133571-70133590 | MS.gene88860:intron | 20.0% |
| !!! | TATAATGGTGCTTATTTTTT+TGG | + | chr1.1:70135013-70135032 | MS.gene88860:intron | 20.0% |
| !!! | TTAATCTTAGTAGCTTTAAA+TGG | + | chr1.1:70134294-70134313 | MS.gene88860:intron | 20.0% |
| !!! | TTATTTTACATCCTAGATAA+AGG | - | chr1.1:70134848-70134867 | None:intergenic | 20.0% |
| ! | TCACATTATATTAGAACTGA+TGG | - | chr1.1:70133559-70133578 | None:intergenic | 25.0% |
| !! | AAGTGTCTTACATCTATTTT+TGG | - | chr1.1:70134417-70134436 | None:intergenic | 25.0% |
| !! | GTGATTCTATTGATATGTTT+TGG | + | chr1.1:70134753-70134772 | MS.gene88860:CDS | 25.0% |
| !!! | AATTTATTTCATTGACTTGG+AGG | + | chr1.1:70134351-70134370 | MS.gene88860:intron | 25.0% |
| !!! | ACAATAGAACACTTTGTTTT+TGG | - | chr1.1:70133609-70133628 | None:intergenic | 25.0% |
| !!! | TATTTTTTTGGATATTCCAG+AGG | + | chr1.1:70135025-70135044 | MS.gene88860:intron | 25.0% |
| AATGACATTGTACCCTATAA+TGG | + | chr1.1:70134998-70135017 | MS.gene88860:intron | 30.0% | |
| AGAATCTAGTTGGATTTATG+CGG | + | chr1.1:70134722-70134741 | MS.gene88860:CDS | 30.0% | |
| AGCGAAGTTAAGTACTATTA+TGG | - | chr1.1:70133814-70133833 | None:intergenic | 30.0% | |
| ATTATATGCAAACTGCTCAT+TGG | + | chr1.1:70133940-70133959 | MS.gene88860:intron | 30.0% | |
| ATTCGAAAACACCTTTATCT+AGG | + | chr1.1:70134834-70134853 | MS.gene88860:intron | 30.0% | |
| GCGAAGTTAAGTACTATTAT+GGG | - | chr1.1:70133813-70133832 | None:intergenic | 30.0% | |
| TCTATGTACTGTCTACTATA+TGG | + | chr1.1:70133888-70133907 | MS.gene88860:intron | 30.0% | |
| TTGCTTACAAAGAATCTAGT+TGG | + | chr1.1:70134712-70134731 | MS.gene88860:CDS | 30.0% | |
| ! | AAGAATCCTTTTCAGCATTT+TGG | + | chr1.1:70133638-70133657 | MS.gene88860:intron | 30.0% |
| ! | AGAATCCTTTTCAGCATTTT+GGG | + | chr1.1:70133639-70133658 | MS.gene88860:intron | 30.0% |
| ! | TGATGTTTTCTATATAGGAG+TGG | + | chr1.1:70133576-70133595 | MS.gene88860:intron | 30.0% |
| ! | TTTTTGTAGACATACTTGTG+TGG | - | chr1.1:70133374-70133393 | None:intergenic | 30.0% |
| !!! | TTCTATTGATATGTTTTGGC+AGG | + | chr1.1:70134757-70134776 | MS.gene88860:CDS | 30.0% |
| ACAAGCTCCAAAATGTTTAC+AGG | + | chr1.1:70134203-70134222 | MS.gene88860:CDS | 35.0% | |
| AGTAGACAGTACATAGAATG+TGG | - | chr1.1:70133885-70133904 | None:intergenic | 35.0% | |
| ATAAGCATGAACTGCTTGAA+TGG | + | chr1.1:70133749-70133768 | MS.gene88860:intron | 35.0% | |
| CATGCTTATATGAGCACTTA+CGG | - | chr1.1:70133738-70133757 | None:intergenic | 35.0% | |
| CCTAATTGAAATATTCGCCA+AGG | + | chr1.1:70134061-70134080 | MS.gene88860:intron | 35.0% | |
| CCTTGGCGAATATTTCAATT+AGG | - | chr1.1:70134064-70134083 | None:intergenic | 35.0% | |
| GTTAAGTACTATTATGGGAG+AGG | - | chr1.1:70133808-70133827 | None:intergenic | 35.0% | |
| ! | TTGGATTTATGCGGTCTTTT+GGG | + | chr1.1:70134731-70134750 | MS.gene88860:CDS | 35.0% |
| !! | AGTACTTAACTTCGCTTTTC+TGG | + | chr1.1:70133818-70133837 | MS.gene88860:intron | 35.0% |
| AGCTCCAAAATGTTTACAGG+TGG | + | chr1.1:70134206-70134225 | MS.gene88860:CDS | 40.0% | |
| ATATTCCAGAGGATGCATGA+AGG | + | chr1.1:70135036-70135055 | MS.gene88860:intron | 40.0% | |
| CAACACCCAAAATGCTGAAA+AGG | - | chr1.1:70133647-70133666 | None:intergenic | 40.0% | |
| CATAGAATGTGGAATGTGAG+TGG | - | chr1.1:70133874-70133893 | None:intergenic | 40.0% | |
| CCAAAATGTTTACAGGTGGA+TGG | + | chr1.1:70134210-70134229 | MS.gene88860:CDS | 40.0% | |
| CTTATGCATAGATTGCAGTG+TGG | + | chr1.1:70133984-70134003 | MS.gene88860:intron | 40.0% | |
| CTTCATTCAATTCCAGGTCT+GGG | + | chr1.1:70134689-70134708 | MS.gene88860:intron | 40.0% | |
| GCAACTCTTCATTCAATTCC+AGG | + | chr1.1:70134683-70134702 | MS.gene88860:intron | 40.0% | |
| GTATACACTCTTGTCACTGA+TGG | + | chr1.1:70133684-70133703 | MS.gene88860:intron | 40.0% | |
| TCTTCATTCAATTCCAGGTC+TGG | + | chr1.1:70134688-70134707 | MS.gene88860:intron | 40.0% | |
| TTATATCCCCGTATTGCACT+TGG | - | chr1.1:70134470-70134489 | None:intergenic | 40.0% | |
| TTATTCCTTCATGCATCCTC+TGG | - | chr1.1:70135044-70135063 | None:intergenic | 40.0% | |
| TTGTCATGCTCATGTGAGTA+AGG | + | chr1.1:70134130-70134149 | MS.gene88860:intron | 40.0% | |
| TTGTGCTTCCAAGTGCAATA+CGG | + | chr1.1:70134459-70134478 | MS.gene88860:intron | 40.0% | |
| ! | AGTGACAAGAGTGTATACCA+AGG | - | chr1.1:70133682-70133701 | None:intergenic | 40.0% |
| ! | CCATCCACCTGTAAACATTT+TGG | - | chr1.1:70134213-70134232 | None:intergenic | 40.0% |
| ! | GCAAACTGCTCATTGGTAAT+TGG | + | chr1.1:70133947-70133966 | MS.gene88860:intron | 40.0% |
| ! | GTTGGATTTATGCGGTCTTT+TGG | + | chr1.1:70134730-70134749 | MS.gene88860:CDS | 40.0% |
| ! | TATATTTGGAGAAAAGGCGC+AGG | + | chr1.1:70133454-70133473 | MS.gene88860:intron | 40.0% |
| ! | TGAGATCTTTTAGTGGTCAC+TGG | - | chr1.1:70134552-70134571 | None:intergenic | 40.0% |
| !! | ATTTTGGGTGTTGTCATGCT+TGG | + | chr1.1:70133654-70133673 | MS.gene88860:intron | 40.0% |
| !! | TCAGCAGACTTGTTTTCGTT+TGG | + | chr1.1:70134649-70134668 | MS.gene88860:intron | 40.0% |
| !!! | GGCAGTCGCTTATCTTTTTT+GGG | + | chr1.1:70134174-70134193 | MS.gene88860:CDS | 40.0% |
| !!! | TGGCAGTCGCTTATCTTTTT+TGG | + | chr1.1:70134173-70134192 | MS.gene88860:CDS | 40.0% |
| TGTGCTTCCAAGTGCAATAC+GGG | + | chr1.1:70134460-70134479 | MS.gene88860:intron | 45.0% | |
| TGTTGTCATGCTTGGAACCT+TGG | + | chr1.1:70133662-70133681 | MS.gene88860:intron | 45.0% | |
| TTATGCACAGCTTCTGCTTG+TGG | + | chr1.1:70134153-70134172 | MS.gene88860:CDS | 45.0% | |
| ! | ACGGAGTCAATAGCTCTGTA+CGG | - | chr1.1:70133719-70133738 | None:intergenic | 45.0% |
| ! | GTCAATAGCTCTGTACGGAA+AGG | - | chr1.1:70133714-70133733 | None:intergenic | 45.0% |
| !!! | ACTTCGCTTTTCTGGTTCAG+TGG | + | chr1.1:70133826-70133845 | MS.gene88860:intron | 45.0% |
| !!! | CTTCGCTTTTCTGGTTCAGT+GGG | + | chr1.1:70133827-70133846 | MS.gene88860:intron | 45.0% |
| !!! | AATAATTTTAATTTTATTAC+AGG | + | chr1.1:70134863-70134882 | MS.gene88860:intron | 5.0% |
| AGACAGTGCATGTGTTGCCT+TGG | - | chr1.1:70134081-70134100 | None:intergenic | 50.0% | |
| CTTTGTAAGCAACCCAGACC+TGG | - | chr1.1:70134704-70134723 | None:intergenic | 50.0% | |
| GTGCTTCCAAGTGCAATACG+GGG | + | chr1.1:70134461-70134480 | MS.gene88860:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 70133354 | 70135063 | 70133354 | ID=MS.gene88860 |
| chr1.1 | mRNA | 70133354 | 70135063 | 70133354 | ID=MS.gene88860.t1;Parent=MS.gene88860 |
| chr1.1 | exon | 70133354 | 70133378 | 70133354 | ID=MS.gene88860.t1.exon1;Parent=MS.gene88860.t1 |
| chr1.1 | CDS | 70133354 | 70133378 | 70133354 | ID=cds.MS.gene88860.t1;Parent=MS.gene88860.t1 |
| chr1.1 | exon | 70134152 | 70134276 | 70134152 | ID=MS.gene88860.t1.exon2;Parent=MS.gene88860.t1 |
| chr1.1 | CDS | 70134152 | 70134276 | 70134152 | ID=cds.MS.gene88860.t1;Parent=MS.gene88860.t1 |
| chr1.1 | exon | 70134705 | 70134778 | 70134705 | ID=MS.gene88860.t1.exon3;Parent=MS.gene88860.t1 |
| chr1.1 | CDS | 70134705 | 70134778 | 70134705 | ID=cds.MS.gene88860.t1;Parent=MS.gene88860.t1 |
| chr1.1 | exon | 70135045 | 70135063 | 70135045 | ID=MS.gene88860.t1.exon4;Parent=MS.gene88860.t1 |
| chr1.1 | CDS | 70135045 | 70135063 | 70135045 | ID=cds.MS.gene88860.t1;Parent=MS.gene88860.t1 |
| Gene Sequence |
| Protein sequence |
