Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056233.t1 | XP_003627843.1 | 95.3 | 192 | 9 | 0 | 1 | 192 | 1 | 192 | 1.30E-98 | 369 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056233.t1 | Q9LK88 | 74.9 | 187 | 47 | 0 | 5 | 191 | 10 | 196 | 1.5e-79 | 297.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056233.t1 | B7FMX0 | 95.3 | 192 | 9 | 0 | 1 | 192 | 1 | 192 | 9.6e-99 | 369.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049757 | MS.gene056233 | 0.814373 | 1.59E-51 | -1.69E-46 |
| MS.gene050060 | MS.gene056233 | 0.861567 | 9.37E-64 | -1.69E-46 |
| MS.gene050426 | MS.gene056233 | -0.820253 | 7.56E-53 | -1.69E-46 |
| MS.gene050670 | MS.gene056233 | 0.803366 | 3.61E-49 | -1.69E-46 |
| MS.gene050971 | MS.gene056233 | 0.809555 | 1.79E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene056233 | 0.817747 | 2.81E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene056233 | 0.80588 | 1.08E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene056233 | 0.811858 | 5.67E-51 | -1.69E-46 |
| MS.gene052469 | MS.gene056233 | 0.831139 | 1.98E-55 | -1.69E-46 |
| MS.gene054349 | MS.gene056233 | 0.805801 | 1.12E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene056233 | 0.803125 | 4.05E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene056659 | 0.824339 | 8.52E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene056899 | -0.806991 | 6.29E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene056979 | 0.806066 | 9.85E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene057396 | 0.828425 | 9.06E-55 | -1.69E-46 |
| MS.gene056233 | MS.gene058928 | 0.814993 | 1.16E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene059420 | 0.801457 | 8.93E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene060247 | 0.804979 | 1.67E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene061159 | 0.830866 | 2.31E-55 | -1.69E-46 |
| MS.gene056233 | MS.gene06260 | 0.815248 | 1.02E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene064519 | 0.803046 | 4.20E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene065475 | 0.813512 | 2.47E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene065846 | 0.812537 | 4.03E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene066009 | 0.803802 | 2.93E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene067385 | 0.807203 | 5.67E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene067925 | 0.809166 | 2.16E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene068064 | 0.807494 | 4.92E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene068946 | 0.837928 | 3.89E-57 | -1.69E-46 |
| MS.gene056233 | MS.gene069717 | 0.810689 | 1.02E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene070790 | 0.834339 | 3.17E-56 | -1.69E-46 |
| MS.gene056233 | MS.gene071546 | 0.802117 | 6.53E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene071722 | 0.819823 | 9.49E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene072070 | 0.833895 | 4.10E-56 | -1.69E-46 |
| MS.gene056233 | MS.gene072659 | -0.81482 | 1.27E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene073427 | 0.830547 | 2.76E-55 | -1.69E-46 |
| MS.gene056233 | MS.gene073446 | 0.800975 | 1.12E-48 | -1.69E-46 |
| MS.gene056233 | MS.gene073723 | 0.808571 | 2.90E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene08083 | 0.837413 | 5.27E-57 | -1.69E-46 |
| MS.gene056233 | MS.gene08779 | 0.818623 | 1.78E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene08906 | 0.835889 | 1.29E-56 | -1.69E-46 |
| MS.gene056233 | MS.gene08908 | 0.840638 | 7.71E-58 | -1.69E-46 |
| MS.gene056233 | MS.gene09137 | 0.805557 | 1.26E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene09310 | 0.82423 | 9.04E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene09765 | 0.822631 | 2.14E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene09777 | 0.83025 | 3.26E-55 | -1.69E-46 |
| MS.gene056233 | MS.gene09923 | 0.802578 | 5.25E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene20096 | 0.856762 | 2.58E-62 | -1.69E-46 |
| MS.gene056233 | MS.gene20340 | 0.812804 | 3.53E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene20556 | 0.82922 | 5.81E-55 | -1.69E-46 |
| MS.gene056233 | MS.gene20812 | 0.821935 | 3.10E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene21716 | 0.808824 | 2.56E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene22386 | 0.842442 | 2.58E-58 | -1.69E-46 |
| MS.gene056233 | MS.gene22721 | 0.800778 | 1.23E-48 | -1.69E-46 |
| MS.gene056233 | MS.gene25929 | 0.807893 | 4.05E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene26624 | -0.821424 | 4.07E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene26705 | -0.828504 | 8.67E-55 | -1.69E-46 |
| MS.gene056233 | MS.gene27363 | 0.838312 | 3.10E-57 | -1.69E-46 |
| MS.gene056233 | MS.gene28314 | 0.826923 | 2.08E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene28693 | 0.823142 | 1.62E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene28694 | 0.819719 | 1.00E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene28853 | -0.81536 | 9.62E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene29968 | 0.815631 | 8.38E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene30520 | -0.812709 | 3.70E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene31266 | 0.820631 | 6.19E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene31671 | 0.839446 | 1.58E-57 | -1.69E-46 |
| MS.gene056233 | MS.gene32713 | 0.819869 | 9.26E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene33198 | 0.810314 | 1.23E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene34187 | 0.807236 | 5.58E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene34188 | 0.818827 | 1.60E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene34190 | 0.845365 | 4.25E-59 | -1.69E-46 |
| MS.gene056233 | MS.gene34191 | 0.822453 | 2.35E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene34800 | 0.808294 | 3.32E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene34845 | 0.826022 | 3.41E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene35020 | 0.842914 | 1.93E-58 | -1.69E-46 |
| MS.gene056233 | MS.gene35381 | 0.8083 | 3.31E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene36105 | 0.807056 | 6.09E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene36260 | 0.809602 | 1.75E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene36507 | 0.81759 | 3.05E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene36952 | 0.81797 | 2.50E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene39277 | 0.809071 | 2.27E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene39361 | 0.819127 | 1.37E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene39366 | 0.807418 | 5.11E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene39679 | 0.819084 | 1.40E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene40169 | 0.824126 | 9.56E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene41649 | 0.81854 | 1.86E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene41713 | 0.826245 | 3.01E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene42746 | 0.810058 | 1.39E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene42747 | 0.815024 | 1.14E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene43149 | 0.835281 | 1.84E-56 | -1.69E-46 |
| MS.gene056233 | MS.gene43782 | 0.802987 | 4.32E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene44654 | 0.828787 | 7.40E-55 | -1.69E-46 |
| MS.gene056233 | MS.gene46956 | 0.821784 | 3.36E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene47714 | 0.851966 | 6.29E-61 | -1.69E-46 |
| MS.gene056233 | MS.gene48030 | 0.852417 | 4.68E-61 | -1.69E-46 |
| MS.gene056233 | MS.gene48573 | 0.835312 | 1.81E-56 | -1.69E-46 |
| MS.gene056233 | MS.gene51007 | -0.801004 | 1.10E-48 | -1.69E-46 |
| MS.gene056233 | MS.gene51034 | 0.89285 | 1.09E-74 | -1.69E-46 |
| MS.gene056233 | MS.gene51540 | 0.813235 | 2.84E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene52872 | 0.815345 | 9.69E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene52997 | 0.809778 | 1.60E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene53143 | 0.803942 | 2.74E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene56177 | 0.854106 | 1.53E-61 | -1.69E-46 |
| MS.gene056233 | MS.gene56713 | 0.826479 | 2.65E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene58344 | 0.804505 | 2.09E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene58345 | 0.818107 | 2.33E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene58480 | 0.813312 | 2.73E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene58570 | 0.813966 | 1.96E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene58652 | 0.816091 | 6.61E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene58671 | 0.804007 | 2.66E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene58789 | 0.818047 | 2.40E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene59760 | 0.814709 | 1.34E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene59925 | 0.809907 | 1.50E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene60124 | 0.835185 | 1.94E-56 | -1.69E-46 |
| MS.gene056233 | MS.gene60286 | 0.80483 | 1.79E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene61544 | 0.810041 | 1.40E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene61701 | 0.803761 | 2.99E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene61958 | 0.8013 | 9.61E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene62885 | -0.816495 | 5.37E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene62886 | -0.815749 | 7.88E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene63882 | 0.805188 | 1.51E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene64133 | 0.865147 | 7.29E-65 | -1.69E-46 |
| MS.gene056233 | MS.gene66207 | 0.810592 | 1.07E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene66208 | 0.808653 | 2.79E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene66702 | 0.812806 | 3.52E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene68525 | 0.836543 | 8.80E-57 | -1.69E-46 |
| MS.gene056233 | MS.gene68526 | 0.825164 | 5.44E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene70368 | 0.832214 | 1.07E-55 | -1.69E-46 |
| MS.gene056233 | MS.gene71558 | 0.805694 | 1.18E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene71924 | -0.854612 | 1.10E-61 | -1.69E-46 |
| MS.gene056233 | MS.gene71927 | -0.81315 | 2.96E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene72054 | 0.814037 | 1.89E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene72924 | -0.812418 | 4.28E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene73446 | 0.817436 | 3.30E-52 | -1.69E-46 |
| MS.gene056233 | MS.gene74032 | -0.803959 | 2.72E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene74558 | 0.820275 | 7.47E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene74594 | 0.825829 | 3.79E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene75493 | 0.812454 | 4.21E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene76634 | 0.815267 | 1.01E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene77877 | 0.812259 | 4.64E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene78431 | 0.823473 | 1.36E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene79020 | 0.827369 | 1.62E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene79479 | 0.824772 | 6.74E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene79482 | 0.823548 | 1.31E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene79962 | -0.803093 | 4.11E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene80547 | 0.821403 | 4.11E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene82533 | 0.800735 | 1.25E-48 | -1.69E-46 |
| MS.gene056233 | MS.gene82939 | 0.827023 | 1.97E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene84200 | 0.830199 | 3.36E-55 | -1.69E-46 |
| MS.gene056233 | MS.gene84544 | 0.803392 | 3.56E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene85860 | 0.814304 | 1.65E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene86339 | 0.804817 | 1.80E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene88498 | 0.898798 | 3.73E-77 | -1.69E-46 |
| MS.gene056233 | MS.gene88768 | 0.836462 | 9.23E-57 | -1.69E-46 |
| MS.gene056233 | MS.gene88860 | 0.819804 | 9.58E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene89074 | 0.810948 | 8.94E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene90178 | 0.826344 | 2.86E-54 | -1.69E-46 |
| MS.gene056233 | MS.gene91276 | 0.811993 | 5.30E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene91509 | 0.814964 | 1.18E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene91581 | 0.82168 | 3.55E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene92106 | 0.806878 | 6.64E-50 | -1.69E-46 |
| MS.gene056233 | MS.gene92773 | 0.802288 | 6.03E-49 | -1.69E-46 |
| MS.gene056233 | MS.gene94333 | 0.801194 | 1.01E-48 | -1.69E-46 |
| MS.gene056233 | MS.gene95707 | 0.821749 | 3.42E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene97081 | 0.820281 | 7.45E-53 | -1.69E-46 |
| MS.gene056233 | MS.gene98609 | 0.811482 | 6.85E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene99877 | 0.827068 | 1.92E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056233.t1 | MTR_8g039090 | 95.312 | 192 | 9 | 0 | 1 | 192 | 1 | 192 | 1.75e-136 | 379 |
| MS.gene056233.t1 | MTR_3g019160 | 75.789 | 190 | 46 | 0 | 3 | 192 | 2 | 191 | 1.20e-108 | 309 |
| MS.gene056233.t1 | MTR_3g019090 | 72.105 | 190 | 53 | 0 | 3 | 192 | 2 | 191 | 2.24e-105 | 300 |
| MS.gene056233.t1 | MTR_3g019150 | 72.105 | 190 | 52 | 1 | 3 | 192 | 2 | 190 | 2.28e-101 | 290 |
| MS.gene056233.t1 | MTR_3g019120 | 62.827 | 191 | 68 | 1 | 3 | 190 | 2 | 192 | 5.01e-86 | 258 |
| MS.gene056233.t1 | MTR_3g019120 | 62.827 | 191 | 68 | 1 | 3 | 190 | 2 | 192 | 8.96e-85 | 259 |
| MS.gene056233.t1 | MTR_3g019090 | 73.239 | 142 | 38 | 0 | 51 | 192 | 1 | 142 | 2.31e-79 | 233 |
| MS.gene056233.t1 | MTR_3g019120 | 66.447 | 152 | 51 | 0 | 39 | 190 | 1 | 152 | 1.47e-72 | 226 |
| MS.gene056233.t1 | MTR_3g019150 | 75.000 | 100 | 25 | 0 | 93 | 192 | 35 | 134 | 2.22e-54 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene056233.t1 | AT3G27890 | 74.866 | 187 | 47 | 0 | 5 | 191 | 10 | 196 | 4.92e-101 | 290 |
Find 37 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCAAAGTGGCAGCTCTTTC+TGG | 0.181158 | 8.1:+65033265 | MS.gene056233:CDS |
| CCATCATCATTGAACTTTAC+AGG | 0.239451 | 8.1:-65034903 | None:intergenic |
| CATCTAGACCACCAAATGTT+TGG | 0.329711 | 8.1:+65034739 | MS.gene056233:CDS |
| CATTGAAGAATGCAATTGAT+TGG | 0.367565 | 8.1:+65034715 | MS.gene056233:CDS |
| ATCTAGACCACCAAATGTTT+GGG | 0.385196 | 8.1:+65034740 | MS.gene056233:CDS |
| CAATCAATTGCATTCTTCAA+TGG | 0.420729 | 8.1:-65034714 | None:intergenic |
| GCATTTACTCTGAGACTTCA+AGG | 0.432779 | 8.1:+65034984 | MS.gene056233:CDS |
| TGGCGAAAAGCTTCAACAAC+AGG | 0.452851 | 8.1:-65033532 | None:intergenic |
| ATTGAAGAATGCAATTGATT+GGG | 0.453647 | 8.1:+65034716 | MS.gene056233:CDS |
| TTTCCAGCCCAAACATTTGG+TGG | 0.464756 | 8.1:-65034747 | None:intergenic |
| ATAAGCACTGGAGGAGACTT+TGG | 0.465806 | 8.1:+65034783 | MS.gene056233:CDS |
| GCACTGGAGGAGACTTTGGC+GGG | 0.469020 | 8.1:+65034787 | MS.gene056233:CDS |
| AAGACGAACCAGCACGGATA+AGG | 0.470569 | 8.1:-65033318 | None:intergenic |
| CCTGTAAAGTTCAATGATGA+TGG | 0.507108 | 8.1:+65034903 | MS.gene056233:CDS |
| CATTCAGGCCTTATCCGTGC+TGG | 0.509003 | 8.1:+65033310 | MS.gene056233:CDS |
| GCAGCAATTGAATTGAGTAA+AGG | 0.518179 | 8.1:+65033421 | MS.gene056233:intron |
| AGACCACCAAATGTTTGGGC+TGG | 0.522737 | 8.1:+65034744 | MS.gene056233:CDS |
| TTGAGTAAAGGAGCAATTGA+AGG | 0.530953 | 8.1:+65033433 | MS.gene056233:CDS |
| GCAGCTGCAATCATAAGCAC+TGG | 0.535634 | 8.1:+65034771 | MS.gene056233:CDS |
| CAGCACGGATAAGGCCTGAA+TGG | 0.546637 | 8.1:-65033309 | None:intergenic |
| CACTATCATCTACGCCAAGT+TGG | 0.576435 | 8.1:+65034819 | MS.gene056233:CDS |
| GAGTATAATTACTCTGTCAC+AGG | 0.578050 | 8.1:+65033595 | MS.gene056233:CDS |
| TCAAGATCAGTGTTGTACAT+TGG | 0.583062 | 8.1:-65033490 | None:intergenic |
| AGCACTGGAGGAGACTTTGG+CGG | 0.584571 | 8.1:+65034786 | MS.gene056233:CDS |
| GATCGATATAAATTCCAACT+TGG | 0.591111 | 8.1:-65034833 | None:intergenic |
| CGAAAGAAGACGAACCAGCA+CGG | 0.596751 | 8.1:-65033324 | None:intergenic |
| CGAAAAGCTTCAACAACAGG+TGG | 0.602230 | 8.1:-65033529 | None:intergenic |
| TGGCGATTTGATTGATGAAG+AGG | 0.612134 | 8.1:+65034923 | MS.gene056233:CDS |
| ACTGGAGGAGACTTTGGCGG+GGG | 0.627120 | 8.1:+65034789 | MS.gene056233:CDS |
| AACACTGATCTTGAAAACAA+AGG | 0.627471 | 8.1:+65033499 | MS.gene056233:CDS |
| AATGCTCTTAGAAAGAACTC+AGG | 0.630349 | 8.1:-65034873 | None:intergenic |
| GTGACAGAGTAATTATACTC+AGG | 0.637539 | 8.1:-65033592 | None:intergenic |
| CACTGGAGGAGACTTTGGCG+GGG | 0.645839 | 8.1:+65034788 | MS.gene056233:CDS |
| GCTGCAATCATAAGCACTGG+AGG | 0.650272 | 8.1:+65034774 | MS.gene056233:CDS |
| TATCAGCTTGAAGAATCTTG+TGG | 0.652286 | 8.1:-65033552 | None:intergenic |
| TCATCATTGAACTTTACAGG+TGG | 0.665769 | 8.1:-65034900 | None:intergenic |
| GGCAGCAGCAGTGATCAAAG+TGG | 0.706403 | 8.1:+65033252 | MS.gene056233:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAAAAAATGATTGAGTA+TGG | - | chr8.1:65033836-65033855 | None:intergenic | 15.0% |
| !!! | TAACTTGTATTTAAATACTA+CGG | - | chr8.1:65034547-65034566 | None:intergenic | 15.0% |
| !!! | TACTCAATCATTTTTTATAA+TGG | + | chr8.1:65033836-65033855 | MS.gene056233:intron | 15.0% |
| !! | AACCGAAAAATTTACAATTA+TGG | - | chr8.1:65034306-65034325 | None:intergenic | 20.0% |
| !! | CAACTATATATATAATACTG+TGG | + | chr8.1:65033745-65033764 | MS.gene056233:intron | 20.0% |
| !! | GTAAACTTAAACTCATATAA+AGG | + | chr8.1:65034573-65034592 | MS.gene056233:intron | 20.0% |
| !! | TATTATATATATAGTTGCAG+AGG | - | chr8.1:65033742-65033761 | None:intergenic | 20.0% |
| !!! | TCATTGACATTTTTAGAATT+AGG | + | chr8.1:65034096-65034115 | MS.gene056233:intron | 20.0% |
| ! | ACTATATCTCTGAATTTAGA+AGG | - | chr8.1:65034608-65034627 | None:intergenic | 25.0% |
| ! | ATTGAAGAATGCAATTGATT+GGG | + | chr8.1:65034716-65034735 | MS.gene056233:CDS | 25.0% |
| ! | CAATTGAGAGACATATAATA+AGG | - | chr8.1:65033948-65033967 | None:intergenic | 25.0% |
| ! | CTATATCTCTGAATTTAGAA+GGG | - | chr8.1:65034607-65034626 | None:intergenic | 25.0% |
| ! | CTTAATTCTCAACATTTCAA+TGG | + | chr8.1:65034343-65034362 | MS.gene056233:intron | 25.0% |
| !! | GGCCATAATTGTAAATTTTT+CGG | + | chr8.1:65034301-65034320 | MS.gene056233:intron | 25.0% |
| AAATGTTGTTCTTGTGTTGA+TGG | + | chr8.1:65033884-65033903 | MS.gene056233:intron | 30.0% | |
| AACACAAGAACAACATTTCA+AGG | - | chr8.1:65033882-65033901 | None:intergenic | 30.0% | |
| AACACTGATCTTGAAAACAA+AGG | + | chr8.1:65033499-65033518 | MS.gene056233:CDS | 30.0% | |
| ACACAAAATAAAGGTGAAGA+TGG | - | chr8.1:65034688-65034707 | None:intergenic | 30.0% | |
| ACACAAGAACAACATTTCAA+GGG | - | chr8.1:65033881-65033900 | None:intergenic | 30.0% | |
| ACTCATTTATATACCTCTCT+AGG | - | chr8.1:65033982-65034001 | None:intergenic | 30.0% | |
| AGAAAAACTTCATACCATTC+AGG | + | chr8.1:65033295-65033314 | MS.gene056233:CDS | 30.0% | |
| ATGGGATATTTGATTGCAAT+TGG | + | chr8.1:65034259-65034278 | MS.gene056233:intron | 30.0% | |
| ATTGCAAAAACAGTTGCATA+TGG | + | chr8.1:65034496-65034515 | MS.gene056233:intron | 30.0% | |
| ATTGCAATCAAATATCCCAT+TGG | - | chr8.1:65034259-65034278 | None:intergenic | 30.0% | |
| CAATCAATTGCATTCTTCAA+TGG | - | chr8.1:65034717-65034736 | None:intergenic | 30.0% | |
| CATTGAAGAATGCAATTGAT+TGG | + | chr8.1:65034715-65034734 | MS.gene056233:CDS | 30.0% | |
| GATCGATATAAATTCCAACT+TGG | - | chr8.1:65034836-65034855 | None:intergenic | 30.0% | |
| TAGACAAGTGATAAAGCTAT+CGG | - | chr8.1:65034377-65034396 | None:intergenic | 30.0% | |
| TCATATATAGACGTAGAAGA+GGG | - | chr8.1:65033812-65033831 | None:intergenic | 30.0% | |
| TGGAGCTAAACACAAAATAA+AGG | - | chr8.1:65034697-65034716 | None:intergenic | 30.0% | |
| ! | ACAAGTTCTTTTATCTTTGC+AGG | + | chr8.1:65034962-65034981 | MS.gene056233:CDS | 30.0% |
| ! | ATGGTATGAAGTTTTTCTGA+AGG | - | chr8.1:65033293-65033312 | None:intergenic | 30.0% |
| ! | GTTGTAGTATTGAATTTGCA+TGG | + | chr8.1:65033386-65033405 | MS.gene056233:intron | 30.0% |
| !! | CTTGTGTTGATGGATTTATT+TGG | + | chr8.1:65033894-65033913 | MS.gene056233:intron | 30.0% |
| !! | TTGATGGATTTATTTGGCTT+TGG | + | chr8.1:65033900-65033919 | MS.gene056233:intron | 30.0% |
| !!! | ATATGCAACTGTTTTTGCAA+TGG | - | chr8.1:65034497-65034516 | None:intergenic | 30.0% |
| !!! | GATTTATTTGGCTTTGGTTT+TGG | + | chr8.1:65033906-65033925 | MS.gene056233:intron | 30.0% |
| AATGCTCTTAGAAAGAACTC+AGG | - | chr8.1:65034876-65034895 | None:intergenic | 35.0% | |
| AGACAAGTGATAAAGCTATC+GGG | - | chr8.1:65034376-65034395 | None:intergenic | 35.0% | |
| ATCTAGACCACCAAATGTTT+GGG | + | chr8.1:65034740-65034759 | MS.gene056233:CDS | 35.0% | |
| CACTTGTCTAGTAATGCATT+AGG | + | chr8.1:65034387-65034406 | MS.gene056233:intron | 35.0% | |
| CATATATAGACGTAGAAGAG+GGG | - | chr8.1:65033811-65033830 | None:intergenic | 35.0% | |
| CCATCATCATTGAACTTTAC+AGG | - | chr8.1:65034906-65034925 | None:intergenic | 35.0% | |
| CCTGTAAAGTTCAATGATGA+TGG | + | chr8.1:65034903-65034922 | MS.gene056233:CDS | 35.0% | |
| CTCATATATAGACGTAGAAG+AGG | - | chr8.1:65033813-65033832 | None:intergenic | 35.0% | |
| GAGTATAATTACTCTGTCAC+AGG | + | chr8.1:65033595-65033614 | MS.gene056233:CDS | 35.0% | |
| GCAGCAATTGAATTGAGTAA+AGG | + | chr8.1:65033421-65033440 | MS.gene056233:intron | 35.0% | |
| GTCAAAGTGAAGGAACTTAA+CGG | + | chr8.1:65034462-65034481 | MS.gene056233:intron | 35.0% | |
| GTGACAGAGTAATTATACTC+AGG | - | chr8.1:65033595-65033614 | None:intergenic | 35.0% | |
| TATCAGCTTGAAGAATCTTG+TGG | - | chr8.1:65033555-65033574 | None:intergenic | 35.0% | |
| TCAAGATCAGTGTTGTACAT+TGG | - | chr8.1:65033493-65033512 | None:intergenic | 35.0% | |
| TCATCATTGAACTTTACAGG+TGG | - | chr8.1:65034903-65034922 | None:intergenic | 35.0% | |
| TTGAGTAAAGGAGCAATTGA+AGG | + | chr8.1:65033433-65033452 | MS.gene056233:CDS | 35.0% | |
| ! | TGCATTAGGAACGTTTTTCT+CGG | + | chr8.1:65034401-65034420 | MS.gene056233:intron | 35.0% |
| !! | AAGAGGAATTTAGTGGATTC+GGG | - | chr8.1:65034198-65034217 | None:intergenic | 35.0% |
| !! | CAAGAGGAATTTAGTGGATT+CGG | - | chr8.1:65034199-65034218 | None:intergenic | 35.0% |
| !! | TTGCAGCTTTGAAATGATCA+CGG | - | chr8.1:65034439-65034458 | None:intergenic | 35.0% |
| AAACAGTTGCATATGGCTCA+CGG | + | chr8.1:65034503-65034522 | MS.gene056233:intron | 40.0% | |
| AAAGTGAAGGAACTTAACGG+TGG | + | chr8.1:65034465-65034484 | MS.gene056233:intron | 40.0% | |
| AAGTGATAAAGCTATCGGGT+AGG | - | chr8.1:65034372-65034391 | None:intergenic | 40.0% | |
| AGTGATAAAGCTATCGGGTA+GGG | - | chr8.1:65034371-65034390 | None:intergenic | 40.0% | |
| CATCTAGACCACCAAATGTT+TGG | + | chr8.1:65034739-65034758 | MS.gene056233:CDS | 40.0% | |
| CCAATTGCAGCTCATACAAA+TGG | + | chr8.1:65034120-65034139 | MS.gene056233:intron | 40.0% | |
| CCATTTGTATGAGCTGCAAT+TGG | - | chr8.1:65034123-65034142 | None:intergenic | 40.0% | |
| CGAATCCACTAAATTCCTCT+TGG | + | chr8.1:65034197-65034216 | MS.gene056233:intron | 40.0% | |
| GCAATTGGATGAGTCTAGTT+TGG | + | chr8.1:65034274-65034293 | MS.gene056233:intron | 40.0% | |
| GCTGCAAATTGTCAAAGTGA+AGG | + | chr8.1:65034452-65034471 | MS.gene056233:intron | 40.0% | |
| GTCTACCAAGAGGAATTTAG+TGG | - | chr8.1:65034205-65034224 | None:intergenic | 40.0% | |
| TGGCGATTTGATTGATGAAG+AGG | + | chr8.1:65034923-65034942 | MS.gene056233:CDS | 40.0% | |
| TGTGAATTGTGAACCTAGAG+AGG | + | chr8.1:65033966-65033985 | MS.gene056233:intron | 40.0% | |
| ! | GCATTAGGAACGTTTTTCTC+GGG | + | chr8.1:65034402-65034421 | MS.gene056233:intron | 40.0% |
| !! | AGAGGAATTTAGTGGATTCG+GGG | - | chr8.1:65034197-65034216 | None:intergenic | 40.0% |
| !! | GCATTTACTCTGAGACTTCA+AGG | + | chr8.1:65034984-65035003 | MS.gene056233:CDS | 40.0% |
| ATCAAAGTGGCAGCTCTTTC+TGG | + | chr8.1:65033265-65033284 | MS.gene056233:CDS | 45.0% | |
| CACTATCATCTACGCCAAGT+TGG | + | chr8.1:65034819-65034838 | MS.gene056233:CDS | 45.0% | |
| CGAAAAGCTTCAACAACAGG+TGG | - | chr8.1:65033532-65033551 | None:intergenic | 45.0% | |
| GAAATGATCACGGTGTCCTT+GGG | - | chr8.1:65034429-65034448 | None:intergenic | 45.0% | |
| TGAAATGATCACGGTGTCCT+TGG | - | chr8.1:65034430-65034449 | None:intergenic | 45.0% | |
| TGGCGAAAAGCTTCAACAAC+AGG | - | chr8.1:65033535-65033554 | None:intergenic | 45.0% | |
| TTTCCAGCCCAAACATTTGG+TGG | - | chr8.1:65034750-65034769 | None:intergenic | 45.0% | |
| ! | GCTTTTCCAGCCCAAACATT+TGG | - | chr8.1:65034753-65034772 | None:intergenic | 45.0% |
| ! | GGATGAGTCTAGTTTGGTCT+AGG | + | chr8.1:65034280-65034299 | MS.gene056233:intron | 45.0% |
| !! | AAATATCCCATTGGTGCCGA+GGG | - | chr8.1:65034250-65034269 | None:intergenic | 45.0% |
| !! | ATAAGCACTGGAGGAGACTT+TGG | + | chr8.1:65034783-65034802 | MS.gene056233:CDS | 45.0% |
| AAGACGAACCAGCACGGATA+AGG | - | chr8.1:65033321-65033340 | None:intergenic | 50.0% | |
| AGACCACCAAATGTTTGGGC+TGG | + | chr8.1:65034744-65034763 | MS.gene056233:CDS | 50.0% | |
| ATACATCCCTCGGCACCAAT+GGG | + | chr8.1:65034241-65034260 | MS.gene056233:intron | 50.0% | |
| ATAGACGTAGAAGAGGGGCT+AGG | - | chr8.1:65033806-65033825 | None:intergenic | 50.0% | |
| CGAAAGAAGACGAACCAGCA+CGG | - | chr8.1:65033327-65033346 | None:intergenic | 50.0% | |
| GACTTTAACCTCGCGTGTGA+AGG | + | chr8.1:65034162-65034181 | MS.gene056233:intron | 50.0% | |
| GCAGCTGCAATCATAAGCAC+TGG | + | chr8.1:65034771-65034790 | MS.gene056233:CDS | 50.0% | |
| TAGACGTAGAAGAGGGGCTA+GGG | - | chr8.1:65033805-65033824 | None:intergenic | 50.0% | |
| TGCCGAGGGATGTATCAATG+CGG | - | chr8.1:65034236-65034255 | None:intergenic | 50.0% | |
| ! | AACGTTTTTCTCGGGTCCCA+AGG | + | chr8.1:65034410-65034429 | MS.gene056233:intron | 50.0% |
| !! | CAAATATCCCATTGGTGCCG+AGG | - | chr8.1:65034251-65034270 | None:intergenic | 50.0% |
| !! | GCTGCAATCATAAGCACTGG+AGG | + | chr8.1:65034774-65034793 | MS.gene056233:CDS | 50.0% |
| AGACGTAGAAGAGGGGCTAG+GGG | - | chr8.1:65033804-65033823 | None:intergenic | 55.0% | |
| AGGGATGTATCAATGCGGGG+AGG | - | chr8.1:65034231-65034250 | None:intergenic | 55.0% | |
| CAGCACGGATAAGGCCTGAA+TGG | - | chr8.1:65033312-65033331 | None:intergenic | 55.0% | |
| CCGAGGGATGTATCAATGCG+GGG | - | chr8.1:65034234-65034253 | None:intergenic | 55.0% | |
| CTAGGGGCGATACGGTTCAA+TGG | - | chr8.1:65033788-65033807 | None:intergenic | 55.0% | |
| GATACATCCCTCGGCACCAA+TGG | + | chr8.1:65034240-65034259 | MS.gene056233:intron | 55.0% | |
| GATTCGAACCTTCACACGCG+AGG | - | chr8.1:65034173-65034192 | None:intergenic | 55.0% | |
| GCCGAGGGATGTATCAATGC+GGG | - | chr8.1:65034235-65034254 | None:intergenic | 55.0% | |
| GGCAGCAGCAGTGATCAAAG+TGG | + | chr8.1:65033252-65033271 | MS.gene056233:CDS | 55.0% | |
| GGGATGTATCAATGCGGGGA+GGG | - | chr8.1:65034230-65034249 | None:intergenic | 55.0% | |
| ! | AGCACTGGAGGAGACTTTGG+CGG | + | chr8.1:65034786-65034805 | MS.gene056233:CDS | 55.0% |
| ! | CATTCAGGCCTTATCCGTGC+TGG | + | chr8.1:65033310-65033329 | MS.gene056233:CDS | 55.0% |
| ! | CCCCGCATTGATACATCCCT+CGG | + | chr8.1:65034231-65034250 | MS.gene056233:intron | 55.0% |
| AAGAGGGGCTAGGGGCGATA+CGG | - | chr8.1:65033796-65033815 | None:intergenic | 60.0% | |
| ACTGGAGGAGACTTTGGCGG+GGG | + | chr8.1:65034789-65034808 | MS.gene056233:CDS | 60.0% | |
| CACTGGAGGAGACTTTGGCG+GGG | + | chr8.1:65034788-65034807 | MS.gene056233:CDS | 60.0% | |
| GCACTGGAGGAGACTTTGGC+GGG | + | chr8.1:65034787-65034806 | MS.gene056233:CDS | 60.0% | |
| GGGGAGGGTTGTCTACCAAG+AGG | - | chr8.1:65034215-65034234 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 65033250 | 65035016 | 65033250 | ID=MS.gene056233 |
| chr8.1 | mRNA | 65033250 | 65035016 | 65033250 | ID=MS.gene056233.t1;Parent=MS.gene056233 |
| chr8.1 | exon | 65033250 | 65033331 | 65033250 | ID=MS.gene056233.t1.exon1;Parent=MS.gene056233.t1 |
| chr8.1 | CDS | 65033250 | 65033331 | 65033250 | ID=cds.MS.gene056233.t1;Parent=MS.gene056233.t1 |
| chr8.1 | exon | 65033425 | 65033616 | 65033425 | ID=MS.gene056233.t1.exon2;Parent=MS.gene056233.t1 |
| chr8.1 | CDS | 65033425 | 65033616 | 65033425 | ID=cds.MS.gene056233.t1;Parent=MS.gene056233.t1 |
| chr8.1 | exon | 65034712 | 65035016 | 65034712 | ID=MS.gene056233.t1.exon3;Parent=MS.gene056233.t1 |
| chr8.1 | CDS | 65034712 | 65035016 | 65034712 | ID=cds.MS.gene056233.t1;Parent=MS.gene056233.t1 |
| Gene Sequence |
| Protein sequence |
