AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89378


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 44 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAGATGAGGCTAAATTAT+AGG 0.245605 7.1:-87872241 MS.gene89378:CDS
CTTAGAAGTTCTTGATAGTT+TGG 0.247944 7.1:+87872689 None:intergenic
GTTTGATGAACTTGAGTTCC+TGG 0.312165 7.1:+87872542 None:intergenic
AAAGGACCATATTGAGCAAT+AGG 0.387063 7.1:+87872329 None:intergenic
AATGAACCTATTGCTCAATA+TGG 0.391955 7.1:-87872335 MS.gene89378:CDS
ACTATTGAAGACTATCAATA+TGG 0.403357 7.1:-87872275 MS.gene89378:CDS
ATAACTCTTCAAAGCCTTTG+AGG 0.406915 7.1:-87872385 MS.gene89378:CDS
CCTTTGAGGTTTGTGCTCAT+TGG 0.408779 7.1:-87872371 MS.gene89378:CDS
AAGGTGATGGACTTAGTGTT+TGG 0.411445 7.1:-87872412 MS.gene89378:CDS
AGTGATAGCAGGAGAATCAA+TGG 0.413770 7.1:-87872624 MS.gene89378:CDS
TCATTTGCATACATAATTGA+AGG 0.440817 7.1:-87872500 MS.gene89378:CDS
CTTGAGTGAAAACAATGATA+TGG 0.447295 7.1:+87872432 None:intergenic
TTGAGGTTTGTGCTCATTGG+TGG 0.448207 7.1:-87872368 MS.gene89378:CDS
AATCAGAACTGGTGATGTTC+AGG 0.449423 7.1:-87873442 MS.gene89378:intron
CTTGATTTCACCATGATGCC+AGG 0.451578 7.1:-87872560 MS.gene89378:CDS
TGAGCAATAGGTTCATTCAA+TGG 0.465412 7.1:+87872341 None:intergenic
CCTTGTGGCCAGCAAAATCT+TGG 0.466878 7.1:+87873469 None:intergenic
ACAAAATTCATCACCAATTG+TGG 0.472578 7.1:-87872459 MS.gene89378:CDS
TTTGTGCAGTGGATGACTGC+AGG 0.482155 7.1:-87873314 MS.gene89378:intron
CACAAGGGTACAATCAGAAC+TGG 0.492820 7.1:-87873453 MS.gene89378:CDS
AAAACAATGGACTGCAATTG+TGG 0.498536 7.1:-87873253 MS.gene89378:CDS
GACTATCAATATGGTAGGAA+TGG 0.507862 7.1:-87872266 MS.gene89378:CDS
CTGGCATCATGGTGAAATCA+AGG 0.508793 7.1:+87872561 None:intergenic
GATTGTGTATTCATGACAAA+AGG 0.515755 7.1:+87872311 None:intergenic
GTTGGTGTTCTTGTGTAGAC+AGG 0.520089 7.1:+87872590 None:intergenic
GTGATAGCAGGAGAATCAAT+GGG 0.534226 7.1:-87872623 MS.gene89378:CDS
ATGATATGGTGAGCCACAAT+TGG 0.536674 7.1:+87872446 None:intergenic
GCAAATGAATTCCATGACTC+AGG 0.546190 7.1:+87872515 None:intergenic
ATCAAACTATTCCTGAGTCA+TGG 0.547660 7.1:-87872526 MS.gene89378:CDS
CAGTTCTGATTGTACCCTTG+TGG 0.562943 7.1:+87873454 None:intergenic
TTGTGCAGTGGATGACTGCA+GGG 0.569971 7.1:-87873313 MS.gene89378:intron
TTGAAGACTATCAATATGGT+AGG 0.576598 7.1:-87872271 MS.gene89378:CDS
GTTGAAGTGAGAGTGATAGC+AGG 0.579971 7.1:-87872635 MS.gene89378:CDS
GCATACATAATTGAAGGAGA+AGG 0.591101 7.1:-87872494 MS.gene89378:CDS
CTAAGTGAGAACATACCATC+GGG 0.591270 7.1:-87872671 MS.gene89378:CDS
GCAGTGGATGACTGCAGGGA+GGG 0.592783 7.1:-87873309 MS.gene89378:intron
AACTTGAGTTCCTGGCATCA+TGG 0.595280 7.1:+87872550 None:intergenic
CAGTGGATGACTGCAGGGAG+GGG 0.597592 7.1:-87873308 MS.gene89378:CDS
CATGGTGAAATCAAGGAACA+TGG 0.604059 7.1:+87872568 None:intergenic
CCAATGAGCACAAACCTCAA+AGG 0.613431 7.1:+87872371 None:intergenic
TCTAAGTGAGAACATACCAT+CGG 0.619506 7.1:-87872672 MS.gene89378:CDS
TGCAGTGGATGACTGCAGGG+AGG 0.648962 7.1:-87873310 MS.gene89378:intron
GACAGTCAGCTACATGCTAG+AGG 0.657626 7.1:-87873726 MS.gene89378:intron
GTGAAATCAAGGAACATGGT+TGG 0.672435 7.1:+87872572 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATAATCTATAATCTAT+AGG - chr7.1:87873092-87873111 MS.gene89378:intron 10.0%
!! ATTATAATCTATAATCTATA+GGG - chr7.1:87873093-87873112 MS.gene89378:intron 10.0%
!! TATACTAAAATTGATTAACT+AGG + chr7.1:87872842-87872861 None:intergenic 15.0%
!!! GCAATTAATTTATTTTCTTT+AGG - chr7.1:87872346-87872365 MS.gene89378:CDS 15.0%
!! AAAAACATAAGAAGCTTAAA+TGG - chr7.1:87872589-87872608 MS.gene89378:CDS 20.0%
!! AATGCAATATATATAGTGTA+AGG - chr7.1:87872955-87872974 MS.gene89378:intron 20.0%
!! AGATTTAGACTTCTTAAATA+AGG + chr7.1:87872915-87872934 None:intergenic 20.0%
!! CTATATATATTGCATTACAT+TGG + chr7.1:87872951-87872970 None:intergenic 20.0%
!! GTAAAAATAATGACCTTAAT+CGG + chr7.1:87873211-87873230 None:intergenic 20.0%
!! TAATCTATAGGGATAAAAAT+TGG - chr7.1:87873104-87873123 MS.gene89378:intron 20.0%
!!! TTATGATCAATTAGTGTTTT+TGG + chr7.1:87872559-87872578 None:intergenic 20.0%
! ACTATTGAAGACTATCAATA+TGG - chr7.1:87873690-87873709 MS.gene89378:intron 25.0%
! ATAAGAAGCTTAAATGGAAT+TGG - chr7.1:87872595-87872614 MS.gene89378:CDS 25.0%
! ATATATGTTATGTCTAGTGT+CGG - chr7.1:87872383-87872402 MS.gene89378:CDS 25.0%
! TCATTTGCATACATAATTGA+AGG - chr7.1:87873465-87873484 MS.gene89378:CDS 25.0%
!! CTTAATCGGTTCAATAATAA+CGG + chr7.1:87873197-87873216 None:intergenic 25.0%
!! TCTAAAAAGTTTTGCATTGT+TGG - chr7.1:87872452-87872471 MS.gene89378:CDS 25.0%
!!! ATGAAAAAAGTATTGTTGGA+AGG + chr7.1:87872871-87872890 None:intergenic 25.0%
!!! CTTTGTTTAACTTTTGATTC+AGG - chr7.1:87873247-87873266 MS.gene89378:CDS 25.0%
!!! GTGAATGAAAAAAGTATTGT+TGG + chr7.1:87872875-87872894 None:intergenic 25.0%
!!! TCTTTTTCAATTCTTCTCTA+GGG - chr7.1:87873056-87873075 MS.gene89378:intron 25.0%
!!! TGAAAAAAGTATTGTTGGAA+GGG + chr7.1:87872870-87872889 None:intergenic 25.0%
AATGAACCTATTGCTCAATA+TGG - chr7.1:87873630-87873649 MS.gene89378:intron 30.0%
ACAAAATTCATCACCAATTG+TGG - chr7.1:87873506-87873525 MS.gene89378:intron 30.0%
CGTTATTATTGAACCGATTA+AGG - chr7.1:87873195-87873214 MS.gene89378:intron 30.0%
CTTGAGTGAAAACAATGATA+TGG + chr7.1:87873536-87873555 None:intergenic 30.0%
GATTGTGTATTCATGACAAA+AGG + chr7.1:87873657-87873676 None:intergenic 30.0%
GCTAAATTATAGGTCTCAAT+AGG - chr7.1:87873734-87873753 MS.gene89378:CDS 30.0%
TTGAAGACTATCAATATGGT+AGG - chr7.1:87873694-87873713 MS.gene89378:intron 30.0%
TTGAGATGAGGCTAAATTAT+AGG - chr7.1:87873724-87873743 MS.gene89378:intron 30.0%
! CATATCATTGTTTTCACTCA+AGG - chr7.1:87873534-87873553 MS.gene89378:intron 30.0%
! CTTAGAAGTTCTTGATAGTT+TGG + chr7.1:87873279-87873298 None:intergenic 30.0%
! TTTTGCATTGTTGGAATTCA+GGG - chr7.1:87872461-87872480 MS.gene89378:CDS 30.0%
!! GAAAAAAGTATTGTTGGAAG+GGG + chr7.1:87872869-87872888 None:intergenic 30.0%
!!! GTCTTTTTCAATTCTTCTCT+AGG - chr7.1:87873055-87873074 MS.gene89378:intron 30.0%
AAAGGACCATATTGAGCAAT+AGG + chr7.1:87873639-87873658 None:intergenic 35.0%
AACGGTTCAAAACTTTGTCA+TGG + chr7.1:87873179-87873198 None:intergenic 35.0%
ATAACTCTTCAAAGCCTTTG+AGG - chr7.1:87873580-87873599 MS.gene89378:intron 35.0%
ATCAAACTATTCCTGAGTCA+TGG - chr7.1:87873439-87873458 MS.gene89378:intron 35.0%
CATGATGAACATTGGAAACA+CGG + chr7.1:87872304-87872323 None:intergenic 35.0%
GACTATCAATATGGTAGGAA+TGG - chr7.1:87873699-87873718 MS.gene89378:intron 35.0%
GCATACATAATTGAAGGAGA+AGG - chr7.1:87873471-87873490 MS.gene89378:CDS 35.0%
GTCAGTATCATGATGAACAT+TGG + chr7.1:87872312-87872331 None:intergenic 35.0%
TCTAAGTGAGAACATACCAT+CGG - chr7.1:87873293-87873312 MS.gene89378:CDS 35.0%
TGAGCAATAGGTTCATTCAA+TGG + chr7.1:87873627-87873646 None:intergenic 35.0%
! ATAACTCACCAAGATTTTGC+TGG - chr7.1:87872488-87872507 MS.gene89378:CDS 35.0%
! ATTGTTTTCACTCAAGGTGA+TGG - chr7.1:87873540-87873559 MS.gene89378:intron 35.0%
! GTTTTGCATTGTTGGAATTC+AGG - chr7.1:87872460-87872479 MS.gene89378:CDS 35.0%
!! AAAACAATGGACTGCAATTG+TGG - chr7.1:87872712-87872731 MS.gene89378:intron 35.0%
!!! ATCTGTCTTTTTTTGTGCAG+TGG - chr7.1:87872640-87872659 MS.gene89378:CDS 35.0%
!!! ATTGAAGGAGAAGGTGTTTT+TGG - chr7.1:87873480-87873499 MS.gene89378:CDS 35.0%
AGTGATAGCAGGAGAATCAA+TGG - chr7.1:87873341-87873360 MS.gene89378:intron 40.0%
ATACCATCGGGAGAAAAAGA+TGG - chr7.1:87873306-87873325 MS.gene89378:CDS 40.0%
ATGATATGGTGAGCCACAAT+TGG + chr7.1:87873522-87873541 None:intergenic 40.0%
CATGGTGAAATCAAGGAACA+TGG + chr7.1:87873400-87873419 None:intergenic 40.0%
CCTGCAGAAGCAAAAAACAA+TGG - chr7.1:87872699-87872718 MS.gene89378:CDS 40.0%
CTAAGTGAGAACATACCATC+GGG - chr7.1:87873294-87873313 MS.gene89378:CDS 40.0%
GCAAATGAATTCCATGACTC+AGG + chr7.1:87873453-87873472 None:intergenic 40.0%
GTGAAATCAAGGAACATGGT+TGG + chr7.1:87873396-87873415 None:intergenic 40.0%
GTGATAGCAGGAGAATCAAT+GGG - chr7.1:87873342-87873361 MS.gene89378:intron 40.0%
GTTTGATGAACTTGAGTTCC+TGG + chr7.1:87873426-87873445 None:intergenic 40.0%
! AATCAGAACTGGTGATGTTC+AGG - chr7.1:87872523-87872542 MS.gene89378:CDS 40.0%
!! AAGGTGATGGACTTAGTGTT+TGG - chr7.1:87873553-87873572 MS.gene89378:intron 40.0%
!!! CCATTGTTTTTTGCTTCTGC+AGG + chr7.1:87872702-87872721 None:intergenic 40.0%
!!! GTAGGAATGGTTTTGAGATG+AGG - chr7.1:87873712-87873731 MS.gene89378:intron 40.0%
AACTTGAGTTCCTGGCATCA+TGG + chr7.1:87873418-87873437 None:intergenic 45.0%
ACATTGGAAACACGGAAGTG+TGG + chr7.1:87872296-87872315 None:intergenic 45.0%
CCAATGAGCACAAACCTCAA+AGG + chr7.1:87873597-87873616 None:intergenic 45.0%
CTGGCATCATGGTGAAATCA+AGG + chr7.1:87873407-87873426 None:intergenic 45.0%
CTTGATTTCACCATGATGCC+AGG - chr7.1:87873405-87873424 MS.gene89378:intron 45.0%
GTTGAAGTGAGAGTGATAGC+AGG - chr7.1:87873330-87873349 MS.gene89378:intron 45.0%
TGCATTGTTGGAATTCAGGG+AGG - chr7.1:87872464-87872483 MS.gene89378:CDS 45.0%
TTGAGGTTTGTGCTCATTGG+TGG - chr7.1:87873597-87873616 MS.gene89378:intron 45.0%
! ACACCATCTTTTTCTCCCGA+TGG + chr7.1:87873312-87873331 None:intergenic 45.0%
! ACGGAAGTGTGGTACAAAGA+TGG + chr7.1:87872285-87872304 None:intergenic 45.0%
! AGAACTGGTGATGTTCAGGT+AGG - chr7.1:87872527-87872546 MS.gene89378:CDS 45.0%
! CAAGATTTTGCTGGCCACAA+GGG - chr7.1:87872497-87872516 MS.gene89378:CDS 45.0%
! CACAAGGGTACAATCAGAAC+TGG - chr7.1:87872512-87872531 MS.gene89378:CDS 45.0%
! CAGTTCTGATTGTACCCTTG+TGG + chr7.1:87872514-87872533 None:intergenic 45.0%
! GTTGGTGTTCTTGTGTAGAC+AGG + chr7.1:87873378-87873397 None:intergenic 45.0%
!! CCTTTGAGGTTTGTGCTCAT+TGG - chr7.1:87873594-87873613 MS.gene89378:intron 45.0%
!! CTATTATAATATAAAATATA+TGG - chr7.1:87873151-87873170 MS.gene89378:intron 5.0%
!!! TGTATTTTTAATTATTTTAT+TGG - chr7.1:87872775-87872794 MS.gene89378:intron 5.0%
CCTTGTGGCCAGCAAAATCT+TGG + chr7.1:87872499-87872518 None:intergenic 50.0%
GACAGTCAGCTACATGCTAG+AGG - chr7.1:87872239-87872258 MS.gene89378:CDS 50.0%
TTGTGCAGTGGATGACTGCA+GGG - chr7.1:87872652-87872671 MS.gene89378:CDS 50.0%
TTTGTGCAGTGGATGACTGC+AGG - chr7.1:87872651-87872670 MS.gene89378:CDS 50.0%
! ACTGGTGATGTTCAGGTAGG+TGG - chr7.1:87872530-87872549 MS.gene89378:CDS 50.0%
! CCAAGATTTTGCTGGCCACA+AGG - chr7.1:87872496-87872515 MS.gene89378:CDS 50.0%
CAGTGGATGACTGCAGGGAG+GGG - chr7.1:87872657-87872676 MS.gene89378:CDS 60.0%
GCAGTGGATGACTGCAGGGA+GGG - chr7.1:87872656-87872675 MS.gene89378:CDS 60.0%
TGCAGTGGATGACTGCAGGG+AGG - chr7.1:87872655-87872674 MS.gene89378:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 87872232 87873755 87872232 ID=MS.gene89378
chr7.1 mRNA 87872232 87873755 87872232 ID=MS.gene89378.t1;Parent=MS.gene89378
chr7.1 exon 87873727 87873755 87873727 ID=MS.gene89378.t1.exon1;Parent=MS.gene89378.t1
chr7.1 CDS 87873727 87873755 87873727 ID=cds.MS.gene89378.t1;Parent=MS.gene89378.t1
chr7.1 exon 87873443 87873505 87873443 ID=MS.gene89378.t1.exon2;Parent=MS.gene89378.t1
chr7.1 CDS 87873443 87873505 87873443 ID=cds.MS.gene89378.t1;Parent=MS.gene89378.t1
chr7.1 exon 87873227 87873327 87873227 ID=MS.gene89378.t1.exon3;Parent=MS.gene89378.t1
chr7.1 CDS 87873227 87873327 87873227 ID=cds.MS.gene89378.t1;Parent=MS.gene89378.t1
chr7.1 exon 87872232 87872718 87872232 ID=MS.gene89378.t1.exon4;Parent=MS.gene89378.t1
chr7.1 CDS 87872232 87872718 87872232 ID=cds.MS.gene89378.t1;Parent=MS.gene89378.t1
Gene Sequence

>MS.gene89378

GTTTTGAGACAGTCAGCTACATGCTAGAGGTATATATCTATGTATATTGACCATCTTTGTACCACACTTCCGTGTTTCCAATGTTCATCATGATACTGACACATTTAATTACATGCAATTAATTTATTTTCTTTAGGCTATTACAAAATCTATATATGTTATGTCTAGTGTCGGTGTCTCTGTGTGTCAGTACTTCGTAGATGTATTGTCATTTTATTTGTCTAAAAAGTTTTGCATTGTTGGAATTCAGGGAGGTATAACTCACCAAGATTTTGCTGGCCACAAGGGTACAATCAGAACTGGTGATGTTCAGGTAGGTGGTTACCAAAAACACTAATTGATCATAAATAGTTATACAAAAACATAAGAAGCTTAAATGGAATTGGTTAGTTCTAATCTTTCTTATATATCTGTCTTTTTTTGTGCAGTGGATGACTGCAGGGAGGGGAATTATTCACTCTGAAATGCCTGCAGAAGCAAAAAACAATGGACTGCAATTGTGGATCAATTTAGCAGCTAATGACAAAATGTATGTAATATTTGTGTATTTTTAATTATTTTATTGGTTTGAAGTATATTTTATCAATAACTAATCTTTGATGTAGAACCTAGTTAATCAATTTTAGTATAGTCTCCCCTTCCAACAATACTTTTTTCATTCACAAAACTCAAACTCGAGACCTTATTTAAGAAGTCTAAATCTAGTTTCACTTGAACCAATGTAATGCAATATATATAGTGTAAGGTTAAATATTTTACATTTTTTGTTTTTAATCTATGTTCTCGTATTTAGTCTTACAAATTACAAAATTGCATATATTTAGTCTTTTTCAATTCTTCTCTAGGGACTAAAAATATAGAATTATAATCTATAATCTATAGGGATAAAAATTGGATACAAAGACTAAAAAAATTGAAGCTATTATAATATAAAATATATGGCACCATGACAAAGTTTTGAACCGTTATTATTGAACCGATTAAGGTCATTATTTTTACTTTTTTTACTAATTTACTTTGTTTAACTTTTGATTCAGGATTGAACCAAACTATCAAGAACTTCTAAGTGAGAACATACCATCGGGAGAAAAAGATGGTGTTGAAGTGAGAGTGATAGCAGGAGAATCAATGGGAGTGAATTCACCTGTCTACACAAGAACACCAACCATGTTCCTTGATTTCACCATGATGCCAGGAACTCAAGTTCATCAAACTATTCCTGAGTCATGGAATTCATTTGCATACATAATTGAAGGAGAAGGTGTTTTTGGTTCACAAAATTCATCACCAATTGTGGCTCACCATATCATTGTTTTCACTCAAGGTGATGGACTTAGTGTTTGGAATAATAACTCTTCAAAGCCTTTGAGGTTTGTGCTCATTGGTGGACAACCATTGAATGAACCTATTGCTCAATATGGTCCTTTTGTCATGAATACACAATCTGAGATTGAGAAGACTATTGAAGACTATCAATATGGTAGGAATGGTTTTGAGATGAGGCTAAATTATAGGTCTCAATAG

Protein sequence

>MS.gene89378.t1

VLRQSATCUREVULTKILLATRVQSELVMFSGULQGGELFTLKCLQKQKTMDCNCGSIUQLMTKULNQTIKNFUVRTYHREKKMVLKUEUUQENQWEUIHLSTQEHQPCSLISPUCQELKFIKLFLSHGIHLHTULKEKVFLVHKIHHQLWLTISLFSLKVMDLVFGIITLQSLUGLCSLVDNHUMNLLLNMVLLSUIHNLRLRRLLKTINMVGMVLRUGUIIGLN