Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91008.t1 | AES88798.2 | 50.7 | 69 | 34 | 0 | 1 | 69 | 147 | 215 | 6.90E-12 | 80.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91008.t1 | G7JTM9 | 50.7 | 69 | 34 | 0 | 1 | 69 | 147 | 215 | 5.0e-12 | 80.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene91008 | 0.809318 | 2.01E-50 | -1.69E-46 |
| MS.gene051594 | MS.gene91008 | 0.805851 | 1.09E-49 | -1.69E-46 |
| MS.gene051613 | MS.gene91008 | 0.803115 | 4.07E-49 | -1.69E-46 |
| MS.gene052447 | MS.gene91008 | 0.891743 | 3.00E-74 | -1.69E-46 |
| MS.gene053082 | MS.gene91008 | 0.810815 | 9.55E-51 | -1.69E-46 |
| MS.gene05329 | MS.gene91008 | 0.839762 | 1.31E-57 | -1.69E-46 |
| MS.gene053396 | MS.gene91008 | 0.814027 | 1.90E-51 | -1.69E-46 |
| MS.gene05414 | MS.gene91008 | 0.80314 | 4.02E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene91008 | 0.84092 | 6.50E-58 | -1.69E-46 |
| MS.gene05423 | MS.gene91008 | 0.800427 | 1.45E-48 | -1.69E-46 |
| MS.gene055228 | MS.gene91008 | 0.82254 | 2.24E-53 | -1.69E-46 |
| MS.gene05531 | MS.gene91008 | 0.822406 | 2.41E-53 | -1.69E-46 |
| MS.gene055818 | MS.gene91008 | 0.816469 | 5.44E-52 | -1.69E-46 |
| MS.gene055952 | MS.gene91008 | 0.814873 | 1.23E-51 | -1.69E-46 |
| MS.gene056272 | MS.gene91008 | 0.809517 | 1.82E-50 | -1.69E-46 |
| MS.gene057021 | MS.gene91008 | 0.808562 | 2.91E-50 | -1.69E-46 |
| MS.gene057598 | MS.gene91008 | 0.806281 | 8.88E-50 | -1.69E-46 |
| MS.gene058294 | MS.gene91008 | 0.827396 | 1.60E-54 | -1.69E-46 |
| MS.gene058979 | MS.gene91008 | 0.804818 | 1.80E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene91008 | 0.803497 | 3.39E-49 | -1.69E-46 |
| MS.gene059458 | MS.gene91008 | 0.826088 | 3.29E-54 | -1.69E-46 |
| MS.gene059459 | MS.gene91008 | 0.814388 | 1.58E-51 | -1.69E-46 |
| MS.gene059460 | MS.gene91008 | 0.818505 | 1.89E-52 | -1.69E-46 |
| MS.gene059584 | MS.gene91008 | 0.801451 | 8.95E-49 | -1.69E-46 |
| MS.gene060134 | MS.gene91008 | 0.84887 | 4.65E-60 | -1.69E-46 |
| MS.gene060135 | MS.gene91008 | 0.820128 | 8.08E-53 | -1.69E-46 |
| MS.gene060634 | MS.gene91008 | -0.813298 | 2.75E-51 | -1.69E-46 |
| MS.gene060918 | MS.gene91008 | 0.803903 | 2.79E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene91008.t1 | MTR_4g062370 | 50.725 | 69 | 34 | 0 | 1 | 69 | 147 | 215 | 3.78e-19 | 82.8 |
| MS.gene91008.t1 | MTR_4g117740 | 65.385 | 52 | 18 | 0 | 18 | 69 | 142 | 193 | 4.06e-19 | 79.7 |
| MS.gene91008.t1 | MTR_1g047540 | 53.623 | 69 | 32 | 0 | 1 | 69 | 128 | 196 | 1.69e-18 | 80.9 |
| MS.gene91008.t1 | MTR_4g032560 | 46.377 | 69 | 37 | 0 | 1 | 69 | 172 | 240 | 8.42e-15 | 69.7 |
| MS.gene91008.t1 | MTR_4g032580 | 44.928 | 69 | 38 | 0 | 1 | 69 | 172 | 240 | 6.42e-14 | 67.4 |
| MS.gene91008.t1 | MTR_5g094740 | 49.275 | 69 | 31 | 1 | 1 | 69 | 695 | 759 | 1.29e-12 | 63.9 |
| MS.gene91008.t1 | MTR_1g069260 | 43.210 | 81 | 34 | 1 | 1 | 69 | 148 | 228 | 2.83e-12 | 62.4 |
| MS.gene91008.t1 | MTR_1g105765 | 40.580 | 69 | 41 | 0 | 1 | 69 | 144 | 212 | 1.57e-11 | 60.5 |
| MS.gene91008.t1 | MTR_1g105780 | 40.580 | 69 | 41 | 0 | 1 | 69 | 144 | 212 | 1.59e-11 | 60.5 |
| MS.gene91008.t1 | MTR_3g452180 | 40.580 | 69 | 41 | 0 | 1 | 69 | 59 | 127 | 1.65e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTTGCCTATAATGGATTT+TGG | 0.152328 | 6.1:+38681375 | MS.gene91008:CDS |
| GCTTCTTCTCGTATGTATAT+TGG | 0.246519 | 6.1:-38681237 | None:intergenic |
| TTGCAGGACCTTGCCTATAA+TGG | 0.266525 | 6.1:+38681368 | MS.gene91008:intron |
| TGGAATACCGTGAATAAAAT+TGG | 0.300264 | 6.1:-38681185 | None:intergenic |
| GAGCTCCCAAAATCCATTAT+AGG | 0.338489 | 6.1:-38681381 | None:intergenic |
| TTTCCTCAACTGCATCCTTT+AGG | 0.367963 | 6.1:-38681616 | None:intergenic |
| CAAAGGATGTTGAAGATGTT+GGG | 0.380354 | 6.1:+38681699 | MS.gene91008:CDS |
| TCTGTCAATTAGAAACTTCA+TGG | 0.396713 | 6.1:-38681149 | None:intergenic |
| ATCCTTTAGGCTAGACTTAG+TGG | 0.399162 | 6.1:-38681603 | None:intergenic |
| ATATCATGTGCGAATGGGTC+TGG | 0.429776 | 6.1:-38681205 | None:intergenic |
| GGGAGCTCGCTCTTGATAAG+AGG | 0.439148 | 6.1:+38681396 | MS.gene91008:CDS |
| TTCATTATATCATGTGCGAA+TGG | 0.483869 | 6.1:-38681211 | None:intergenic |
| ACAAAGGATGTTGAAGATGT+TGG | 0.507209 | 6.1:+38681698 | MS.gene91008:CDS |
| TCATTATATCATGTGCGAAT+GGG | 0.512869 | 6.1:-38681210 | None:intergenic |
| CTCCACTAAGTCTAGCCTAA+AGG | 0.520342 | 6.1:+38681601 | MS.gene91008:CDS |
| AGAGGGCTACTGATAGTGTT+GGG | 0.531513 | 6.1:+38681669 | MS.gene91008:CDS |
| ATACGAGAAGAAGCTAGATT+CGG | 0.539474 | 6.1:+38681246 | MS.gene91008:CDS |
| GAGGACTACTTCATTGAAGA+TGG | 0.542980 | 6.1:+38681415 | MS.gene91008:CDS |
| AAGAGGGCTACTGATAGTGT+TGG | 0.545138 | 6.1:+38681668 | MS.gene91008:CDS |
| TAGTGTTGGGTCTGCTACAA+AGG | 0.545793 | 6.1:+38681682 | MS.gene91008:CDS |
| CAGGAAAGAATTGTTGAGTA+CGG | 0.555148 | 6.1:+38681575 | MS.gene91008:intron |
| GATTCGGATGAGATTAAGAA+TGG | 0.558096 | 6.1:+38681262 | MS.gene91008:CDS |
| CCCAAAATCCATTATAGGCA+AGG | 0.559867 | 6.1:-38681376 | None:intergenic |
| TAGCCTAAAGGATGCAGTTG+AGG | 0.568977 | 6.1:+38681613 | MS.gene91008:CDS |
| AAAGGATGTTGAAGATGTTG+GGG | 0.594085 | 6.1:+38681700 | MS.gene91008:CDS |
| GAAGATGGTGTTAACAGTCA+TGG | 0.595634 | 6.1:+38681430 | MS.gene91008:CDS |
| CTGTCAATTAGAAACTTCAT+GGG | 0.621525 | 6.1:-38681148 | None:intergenic |
| TGAGATTAAGAATGGTGTGA+TGG | 0.627010 | 6.1:+38681270 | MS.gene91008:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAACACTTAATGAATT+AGG | - | chr6.1:38681534-38681553 | None:intergenic | 20.0% |
| !!! | AAAATTCTAAAGTTGTTAAC+AGG | - | chr6.1:38681350-38681369 | None:intergenic | 20.0% |
| !!! | AAATTCTAAAGTTGTTAACA+GGG | - | chr6.1:38681349-38681368 | None:intergenic | 20.0% |
| !!! | ATATATGCCAATTTTATTCA+CGG | + | chr6.1:38681178-38681197 | MS.gene91008:CDS | 20.0% |
| !!! | GAGAATGTTAGATTTTTCAA+TGG | - | chr6.1:38681480-38681499 | None:intergenic | 25.0% |
| !!! | TAACAACTTTAGAATTTTGC+AGG | + | chr6.1:38681352-38681371 | MS.gene91008:intron | 25.0% |
| !!! | TTTGTTTTTTTGCTACTTAC+AGG | + | chr6.1:38681556-38681575 | MS.gene91008:intron | 25.0% |
| CCTAAAACTAAAAAGATCGT+TGG | + | chr6.1:38681644-38681663 | MS.gene91008:CDS | 30.0% | |
| TCATTATATCATGTGCGAAT+GGG | - | chr6.1:38681213-38681232 | None:intergenic | 30.0% | |
| TGGAATACCGTGAATAAAAT+TGG | - | chr6.1:38681188-38681207 | None:intergenic | 30.0% | |
| TTCATTATATCATGTGCGAA+TGG | - | chr6.1:38681214-38681233 | None:intergenic | 30.0% | |
| ! | TAAAAAGATCGTTGGTAAGA+GGG | + | chr6.1:38681652-38681671 | MS.gene91008:CDS | 30.0% |
| ! | TCGTTTTATATCTGTTTCGT+TGG | + | chr6.1:38681307-38681326 | MS.gene91008:intron | 30.0% |
| !!! | CCAACGATCTTTTTAGTTTT+AGG | - | chr6.1:38681647-38681666 | None:intergenic | 30.0% |
| !!! | GATCTTTTTAGTTTTAGGTG+TGG | - | chr6.1:38681642-38681661 | None:intergenic | 30.0% |
| AAAGGATGTTGAAGATGTTG+GGG | + | chr6.1:38681700-38681719 | MS.gene91008:CDS | 35.0% | |
| AATGAATTAGGTCTCAGTGA+TGG | - | chr6.1:38681522-38681541 | None:intergenic | 35.0% | |
| ACAAAGGATGTTGAAGATGT+TGG | + | chr6.1:38681698-38681717 | MS.gene91008:CDS | 35.0% | |
| ATACGAGAAGAAGCTAGATT+CGG | + | chr6.1:38681246-38681265 | MS.gene91008:CDS | 35.0% | |
| ATTAAGAATGGTGTGATGGT+AGG | + | chr6.1:38681274-38681293 | MS.gene91008:intron | 35.0% | |
| CAAAGGATGTTGAAGATGTT+GGG | + | chr6.1:38681699-38681718 | MS.gene91008:CDS | 35.0% | |
| CAGGAAAGAATTGTTGAGTA+CGG | + | chr6.1:38681575-38681594 | MS.gene91008:intron | 35.0% | |
| GATTCGGATGAGATTAAGAA+TGG | + | chr6.1:38681262-38681281 | MS.gene91008:CDS | 35.0% | |
| GCTTCTTCTCGTATGTATAT+TGG | - | chr6.1:38681240-38681259 | None:intergenic | 35.0% | |
| TGAGATTAAGAATGGTGTGA+TGG | + | chr6.1:38681270-38681289 | MS.gene91008:CDS | 35.0% | |
| TTAAGAATGGTGTGATGGTA+GGG | + | chr6.1:38681275-38681294 | MS.gene91008:intron | 35.0% | |
| ! | CTAAAAAGATCGTTGGTAAG+AGG | + | chr6.1:38681651-38681670 | MS.gene91008:CDS | 35.0% |
| !!! | ACCTTGCCTATAATGGATTT+TGG | + | chr6.1:38681375-38681394 | MS.gene91008:CDS | 35.0% |
| !!! | CCTTGCCTATAATGGATTTT+GGG | + | chr6.1:38681376-38681395 | MS.gene91008:CDS | 35.0% |
| ATCCTTTAGGCTAGACTTAG+TGG | - | chr6.1:38681606-38681625 | None:intergenic | 40.0% | |
| CCCAAAATCCATTATAGGCA+AGG | - | chr6.1:38681379-38681398 | None:intergenic | 40.0% | |
| GAGCTCCCAAAATCCATTAT+AGG | - | chr6.1:38681384-38681403 | None:intergenic | 40.0% | |
| GAGGACTACTTCATTGAAGA+TGG | + | chr6.1:38681415-38681434 | MS.gene91008:CDS | 40.0% | |
| TGGTACAAACCTATTGTCTG+CGG | + | chr6.1:38681450-38681469 | MS.gene91008:intron | 40.0% | |
| TTTCCTCAACTGCATCCTTT+AGG | - | chr6.1:38681619-38681638 | None:intergenic | 40.0% | |
| ! | GAAGATGGTGTTAACAGTCA+TGG | + | chr6.1:38681430-38681449 | MS.gene91008:CDS | 40.0% |
| AATGGTTCACCGCAGACAAT+AGG | - | chr6.1:38681462-38681481 | None:intergenic | 45.0% | |
| ATATCATGTGCGAATGGGTC+TGG | - | chr6.1:38681208-38681227 | None:intergenic | 45.0% | |
| CTCCACTAAGTCTAGCCTAA+AGG | + | chr6.1:38681601-38681620 | MS.gene91008:CDS | 45.0% | |
| TAGCCTAAAGGATGCAGTTG+AGG | + | chr6.1:38681613-38681632 | MS.gene91008:CDS | 45.0% | |
| TTGCAGGACCTTGCCTATAA+TGG | + | chr6.1:38681368-38681387 | MS.gene91008:intron | 45.0% | |
| ! | AAGAGGGCTACTGATAGTGT+TGG | + | chr6.1:38681668-38681687 | MS.gene91008:CDS | 45.0% |
| ! | AGAGGGCTACTGATAGTGTT+GGG | + | chr6.1:38681669-38681688 | MS.gene91008:CDS | 45.0% |
| ! | TAGTGTTGGGTCTGCTACAA+AGG | + | chr6.1:38681682-38681701 | MS.gene91008:CDS | 45.0% |
| GGGAGCTCGCTCTTGATAAG+AGG | + | chr6.1:38681396-38681415 | MS.gene91008:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 38681151 | 38681781 | 38681151 | ID=MS.gene91008 |
| chr6.1 | mRNA | 38681151 | 38681781 | 38681151 | ID=MS.gene91008.t1;Parent=MS.gene91008 |
| chr6.1 | exon | 38681151 | 38681291 | 38681151 | ID=MS.gene91008.t1.exon1;Parent=MS.gene91008.t1 |
| chr6.1 | CDS | 38681151 | 38681291 | 38681151 | ID=cds.MS.gene91008.t1;Parent=MS.gene91008.t1 |
| chr6.1 | exon | 38681374 | 38681451 | 38681374 | ID=MS.gene91008.t1.exon2;Parent=MS.gene91008.t1 |
| chr6.1 | CDS | 38681374 | 38681451 | 38681374 | ID=cds.MS.gene91008.t1;Parent=MS.gene91008.t1 |
| chr6.1 | exon | 38681578 | 38681781 | 38681578 | ID=MS.gene91008.t1.exon3;Parent=MS.gene91008.t1 |
| chr6.1 | CDS | 38681578 | 38681781 | 38681578 | ID=cds.MS.gene91008.t1;Parent=MS.gene91008.t1 |
| Gene Sequence |
| Protein sequence |
