Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93478.t1 | XP_013452105.1 | 98.7 | 75 | 1 | 0 | 1 | 75 | 1 | 75 | 1.00E-26 | 129.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93478.t1 | O03982 | 52.1 | 73 | 33 | 2 | 3 | 74 | 2 | 73 | 5.6e-10 | 65.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93478.t1 | A0A072UAJ9 | 98.7 | 75 | 1 | 0 | 1 | 75 | 1 | 75 | 7.3e-27 | 129.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene93478 | 0.835638 | 1.49E-56 | -1.69E-46 |
| MS.gene049293 | MS.gene93478 | -0.807787 | 4.26E-50 | -1.69E-46 |
| MS.gene049499 | MS.gene93478 | 0.801782 | 7.65E-49 | -1.69E-46 |
| MS.gene050752 | MS.gene93478 | 0.812927 | 3.31E-51 | -1.69E-46 |
| MS.gene050945 | MS.gene93478 | 0.820925 | 5.30E-53 | -1.69E-46 |
| MS.gene050955 | MS.gene93478 | 0.806639 | 7.46E-50 | -1.69E-46 |
| MS.gene051233 | MS.gene93478 | 0.800043 | 1.73E-48 | -1.69E-46 |
| MS.gene051234 | MS.gene93478 | 0.806363 | 8.53E-50 | -1.69E-46 |
| MS.gene051343 | MS.gene93478 | 0.833012 | 6.81E-56 | -1.69E-46 |
| MS.gene05188 | MS.gene93478 | 0.824362 | 8.41E-54 | -1.69E-46 |
| MS.gene051902 | MS.gene93478 | 0.834943 | 2.24E-56 | -1.69E-46 |
| MS.gene051975 | MS.gene93478 | 0.818103 | 2.33E-52 | -1.69E-46 |
| MS.gene052072 | MS.gene93478 | 0.809036 | 2.31E-50 | -1.69E-46 |
| MS.gene053058 | MS.gene93478 | 0.800428 | 1.45E-48 | -1.69E-46 |
| MS.gene054403 | MS.gene93478 | 0.81773 | 2.83E-52 | -1.69E-46 |
| MS.gene05469 | MS.gene93478 | 0.808738 | 2.67E-50 | -1.69E-46 |
| MS.gene055513 | MS.gene93478 | 0.816859 | 4.45E-52 | -1.69E-46 |
| MS.gene057102 | MS.gene93478 | 0.808021 | 3.80E-50 | -1.69E-46 |
| MS.gene057999 | MS.gene93478 | 0.801257 | 9.81E-49 | -1.69E-46 |
| MS.gene059146 | MS.gene93478 | 0.827074 | 1.91E-54 | -1.69E-46 |
| MS.gene05985 | MS.gene93478 | -0.818645 | 1.76E-52 | -1.69E-46 |
| MS.gene059901 | MS.gene93478 | -0.80049 | 1.41E-48 | -1.69E-46 |
| MS.gene06011 | MS.gene93478 | 0.814551 | 1.45E-51 | -1.69E-46 |
| MS.gene060244 | MS.gene93478 | 0.81779 | 2.75E-52 | -1.69E-46 |
| MS.gene060365 | MS.gene93478 | 0.822804 | 1.95E-53 | -1.69E-46 |
| MS.gene060763 | MS.gene93478 | 0.818721 | 1.69E-52 | -1.69E-46 |
| MS.gene060935 | MS.gene93478 | 0.808482 | 3.03E-50 | -1.69E-46 |
| MS.gene060937 | MS.gene93478 | 0.822696 | 2.06E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93478.t1 | MTR_6g045257 | 99.083 | 109 | 1 | 0 | 1 | 109 | 1 | 109 | 6.49e-63 | 187 |
| MS.gene93478.t1 | MTR_1g100587 | 86.111 | 72 | 10 | 0 | 3 | 74 | 4 | 75 | 9.75e-26 | 92.4 |
| MS.gene93478.t1 | MTR_8g098915 | 50.704 | 71 | 34 | 1 | 4 | 74 | 3 | 72 | 2.54e-17 | 72.0 |
| MS.gene93478.t1 | MTR_7g079110 | 53.731 | 67 | 30 | 1 | 4 | 70 | 3 | 68 | 1.39e-15 | 68.6 |
| MS.gene93478.t1 | MTR_3g434960 | 47.368 | 76 | 39 | 1 | 4 | 79 | 3 | 77 | 2.93e-15 | 66.2 |
| MS.gene93478.t1 | MTR_7g451210 | 50.000 | 74 | 36 | 1 | 4 | 77 | 3 | 75 | 3.18e-15 | 66.2 |
| MS.gene93478.t1 | MTR_3g434890 | 47.368 | 76 | 39 | 1 | 4 | 79 | 3 | 77 | 3.37e-15 | 66.2 |
| MS.gene93478.t1 | MTR_6g049320 | 48.611 | 72 | 36 | 1 | 1 | 72 | 30 | 100 | 1.97e-14 | 65.1 |
| MS.gene93478.t1 | MTR_6g049260 | 45.333 | 75 | 40 | 1 | 4 | 78 | 3 | 76 | 3.30e-14 | 63.5 |
| MS.gene93478.t1 | MTR_1173s0010 | 46.053 | 76 | 40 | 1 | 4 | 79 | 3 | 77 | 4.05e-14 | 63.2 |
| MS.gene93478.t1 | MTR_7g451200 | 46.053 | 76 | 40 | 1 | 4 | 79 | 3 | 77 | 4.14e-14 | 63.2 |
| MS.gene93478.t1 | MTR_1g099810 | 49.296 | 71 | 35 | 1 | 4 | 74 | 3 | 72 | 6.55e-14 | 63.5 |
| MS.gene93478.t1 | MTR_6g049320 | 49.275 | 69 | 34 | 1 | 4 | 72 | 3 | 70 | 6.73e-14 | 63.2 |
| MS.gene93478.t1 | MTR_1g099800 | 49.296 | 71 | 35 | 1 | 4 | 74 | 3 | 72 | 7.78e-14 | 63.2 |
| MS.gene93478.t1 | MTR_6g049280 | 47.222 | 72 | 37 | 1 | 1 | 72 | 30 | 100 | 9.19e-14 | 63.5 |
| MS.gene93478.t1 | MTR_6g051680 | 46.575 | 73 | 38 | 1 | 3 | 75 | 2 | 73 | 1.26e-13 | 62.0 |
| MS.gene93478.t1 | MTR_6g049280 | 47.826 | 69 | 35 | 1 | 4 | 72 | 3 | 70 | 1.97e-13 | 62.0 |
| MS.gene93478.t1 | MTR_3g435000 | 41.975 | 81 | 45 | 1 | 1 | 79 | 1 | 81 | 3.32e-13 | 60.8 |
| MS.gene93478.t1 | MTR_6g051690 | 43.836 | 73 | 40 | 1 | 3 | 75 | 2 | 73 | 6.52e-13 | 60.8 |
| MS.gene93478.t1 | MTR_0795s0020 | 47.143 | 70 | 30 | 2 | 3 | 72 | 2 | 64 | 1.14e-11 | 57.4 |
| MS.gene93478.t1 | MTR_8g098915 | 49.020 | 51 | 26 | 0 | 24 | 74 | 3 | 53 | 1.28e-11 | 57.0 |
| MS.gene93478.t1 | MTR_1g099640 | 49.315 | 73 | 36 | 1 | 3 | 75 | 2 | 73 | 2.51e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93478.t1 | AT5G23760 | 88.000 | 75 | 9 | 0 | 1 | 75 | 1 | 75 | 1.91e-28 | 99.8 |
| MS.gene93478.t1 | AT5G23760 | 85.938 | 64 | 9 | 0 | 12 | 75 | 1 | 64 | 7.45e-21 | 80.1 |
| MS.gene93478.t1 | AT1G01490 | 52.857 | 70 | 31 | 2 | 4 | 72 | 3 | 71 | 9.28e-16 | 69.3 |
| MS.gene93478.t1 | AT1G01490 | 52.857 | 70 | 31 | 2 | 4 | 72 | 3 | 71 | 9.28e-16 | 69.3 |
| MS.gene93478.t1 | AT1G01490 | 50.685 | 73 | 34 | 2 | 1 | 72 | 27 | 98 | 1.14e-15 | 69.7 |
| MS.gene93478.t1 | AT5G52740 | 48.571 | 70 | 33 | 2 | 3 | 72 | 2 | 68 | 7.99e-11 | 55.1 |
Find 10 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGCAGAAGGTTGTGTTAA+AGG | 0.218749 | 6.4:+29522121 | MS.gene93478:intron |
| GAGCAAAAGTTGACAGTGAT+TGG | 0.365516 | 6.4:+29522346 | MS.gene93478:CDS |
| GAAGAAAGAAGAAAAGAAAG+AGG | 0.393779 | 6.4:+29522453 | MS.gene93478:CDS |
| AGAAAAGAAAGAGGAGAAGA+AGG | 0.397115 | 6.4:+29522462 | MS.gene93478:CDS |
| GAAGCAGCTGCAGATATCTT+TGG | 0.401330 | 6.4:+29522185 | MS.gene93478:CDS |
| GTTGACAGTGATTGGTTCAA+TGG | 0.405513 | 6.4:+29522354 | MS.gene93478:CDS |
| AGAGAAGAAAGAAGAGAAGA+AGG | 0.411074 | 6.4:+29522510 | MS.gene93478:CDS |
| ATCTCCTCTTCAAATGGCTC+AGG | 0.482301 | 6.4:+29521912 | None:intergenic |
| GAAGAAAGAAGAGAAGAAGG+AGG | 0.516884 | 6.4:+29522513 | MS.gene93478:CDS |
| AAAGTGGATATTGTATCTGT+TGG | 0.556535 | 6.4:+29522415 | MS.gene93478:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTAAATTAAATAGGAT+AGG | - | chr6.4:29521957-29521976 | None:intergenic | 10.0% |
| !! | AGAACAAAATTAAATTAAAT+AGG | - | chr6.4:29521962-29521981 | None:intergenic | 10.0% |
| !!! | AATGTTATTTAATAAATCAA+AGG | + | chr6.4:29522258-29522277 | MS.gene93478:intron | 10.0% |
| !! | AATAAGAGAATGAAAAAAAA+AGG | - | chr6.4:29522083-29522102 | None:intergenic | 15.0% |
| !! | ATTTATATAGCTAAATCATG+AGG | - | chr6.4:29522003-29522022 | None:intergenic | 20.0% |
| ! | AAATCATGAGGATTTACATA+AGG | - | chr6.4:29521991-29522010 | None:intergenic | 25.0% |
| ! | TCATTCTCTTATTCTTTCAT+TGG | + | chr6.4:29522090-29522109 | MS.gene93478:intron | 25.0% |
| AAAGTGGATATTGTATCTGT+TGG | + | chr6.4:29522415-29522434 | MS.gene93478:CDS | 30.0% | |
| AATAGGATAGGATATGAAAG+AGG | - | chr6.4:29521945-29521964 | None:intergenic | 30.0% | |
| AGAGAAAAAAGAAGAGAAGA+AGG | + | chr6.4:29522486-29522505 | MS.gene93478:CDS | 30.0% | |
| GAAGAAAGAAGAAAAGAAAG+AGG | + | chr6.4:29522453-29522472 | MS.gene93478:CDS | 30.0% | |
| !! | TGTTGTGGATTTTGATGTAT+AGG | + | chr6.4:29522295-29522314 | MS.gene93478:intron | 30.0% |
| AAAGCAGAAGGTTGTGTTAA+AGG | + | chr6.4:29522121-29522140 | MS.gene93478:intron | 35.0% | |
| AGAAAAGAAAGAGGAGAAGA+AGG | + | chr6.4:29522462-29522481 | MS.gene93478:CDS | 35.0% | |
| AGAGAAGAAAGAAGAGAAGA+AGG | + | chr6.4:29522510-29522529 | MS.gene93478:CDS | 35.0% | |
| GCTGAAAAAAGTAGGAAAAG+TGG | + | chr6.4:29522399-29522418 | MS.gene93478:CDS | 35.0% | |
| GCTGATCTATTGAATTGTTG+TGG | + | chr6.4:29522280-29522299 | MS.gene93478:intron | 35.0% | |
| GTGAAGAAGCTGAAAAAAGT+AGG | + | chr6.4:29522391-29522410 | MS.gene93478:CDS | 35.0% | |
| ! | TCTTTCTTCTCTTCTTTTGC+AGG | - | chr6.4:29522442-29522461 | None:intergenic | 35.0% |
| ! | TGCTTCTATAGCTTTTTGCT+TGG | - | chr6.4:29522171-29522190 | None:intergenic | 35.0% |
| ! | TTGGATTGATTCAAAGCAGA+AGG | + | chr6.4:29522109-29522128 | MS.gene93478:intron | 35.0% |
| GAAGAAAGAAGAGAAGAAGG+AGG | + | chr6.4:29522513-29522532 | MS.gene93478:CDS | 40.0% | |
| GAGCAAAAGTTGACAGTGAT+TGG | + | chr6.4:29522346-29522365 | MS.gene93478:CDS | 40.0% | |
| ! | GTTGACAGTGATTGGTTCAA+TGG | + | chr6.4:29522354-29522373 | MS.gene93478:CDS | 40.0% |
| GAAGCAGCTGCAGATATCTT+TGG | + | chr6.4:29522185-29522204 | MS.gene93478:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 29521925 | 29522558 | 29521925 | ID=MS.gene93478 |
| chr6.4 | mRNA | 29521925 | 29522558 | 29521925 | ID=MS.gene93478.t1;Parent=MS.gene93478 |
| chr6.4 | exon | 29521925 | 29521933 | 29521925 | ID=MS.gene93478.t1.exon1;Parent=MS.gene93478.t1 |
| chr6.4 | CDS | 29521925 | 29521933 | 29521925 | ID=cds.MS.gene93478.t1;Parent=MS.gene93478.t1 |
| chr6.4 | exon | 29522128 | 29522206 | 29522128 | ID=MS.gene93478.t1.exon2;Parent=MS.gene93478.t1 |
| chr6.4 | CDS | 29522128 | 29522206 | 29522128 | ID=cds.MS.gene93478.t1;Parent=MS.gene93478.t1 |
| chr6.4 | exon | 29522317 | 29522558 | 29522317 | ID=MS.gene93478.t1.exon3;Parent=MS.gene93478.t1 |
| chr6.4 | CDS | 29522317 | 29522558 | 29522317 | ID=cds.MS.gene93478.t1;Parent=MS.gene93478.t1 |
| Gene Sequence |
| Protein sequence |
