AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048029.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048029.01.T01 MTR_4g105310 94.479 163 9 0 1 163 1 163 2.26e-109 313
MsG0080048029.01.T01 MTR_4g105310 94.479 163 9 0 1 163 1 163 1.04e-108 315
MsG0080048029.01.T01 MTR_3g077410 83.553 152 25 0 12 163 1 152 2.21e-90 268
MsG0080048029.01.T01 MTR_8g099995 55.346 159 68 2 12 170 1 156 2.42e-51 168
MsG0080048029.01.T01 MTR_7g099850 51.807 166 75 2 12 175 1 163 1.06e-48 161
MsG0080048029.01.T01 MTR_1g068970 51.205 166 76 2 12 175 1 163 3.21e-48 159
MsG0080048029.01.T01 MTR_8g098415 50.000 146 70 1 18 163 2 144 8.22e-44 145
MsG0080048029.01.T01 MTR_8g098415 50.000 146 70 1 18 163 2 144 7.04e-43 145
MsG0080048029.01.T01 MTR_8g098415 50.000 146 70 1 18 163 2 144 8.07e-43 145
MsG0080048029.01.T01 MTR_1g100305 52.857 140 63 1 24 163 8 144 3.46e-41 141
MsG0080048029.01.T01 MTR_1g100395 54.286 140 61 1 24 163 8 144 4.93e-41 141
MsG0080048029.01.T01 MTR_6g053600 53.623 138 61 1 26 163 4 138 1.54e-40 139
MsG0080048029.01.T01 MTR_1g100335 52.857 140 63 1 24 163 8 144 7.25e-40 136
MsG0080048029.01.T01 MTR_1g100335 52.857 140 63 1 24 163 8 144 1.47e-39 136
MsG0080048029.01.T01 MTR_1g100335 52.857 140 63 1 24 163 8 144 2.31e-39 136
MsG0080048029.01.T01 MTR_6g053550 51.799 139 64 1 25 163 17 152 1.63e-38 134
MsG0080048029.01.T01 MTR_1g100315 50.714 140 66 1 24 163 8 144 1.54e-37 132
MsG0080048029.01.T01 MTR_8g022500 51.587 126 36 1 39 164 417 517 7.96e-32 120
MsG0080048029.01.T01 MTR_8g098415 47.126 87 46 0 77 163 1 87 9.34e-21 86.7
MsG0080048029.01.T01 MTR_1g068970 44.792 96 51 1 82 175 1 96 2.51e-19 82.8
MsG0080048029.01.T01 MTR_6g081020 39.844 128 67 3 40 157 51 178 2.06e-17 79.3
MsG0080048029.01.T01 MTR_6g041980 33.333 132 83 1 51 177 69 200 2.32e-16 75.1
MsG0080048029.01.T01 MTR_6g041980 33.588 131 82 1 52 177 70 200 2.71e-16 75.9
MsG0080048029.01.T01 MTR_2g079710 37.607 117 65 4 50 161 88 201 1.79e-15 73.6
MsG0080048029.01.T01 MTR_1g100345 39.726 73 44 0 49 121 1 73 1.63e-12 60.8
MsG0080048029.01.T01 MTR_7g076660 40.000 95 49 2 51 141 10 100 5.14e-12 63.2
MsG0080048029.01.T01 MTR_7g076660 40.000 95 49 2 51 141 9 99 6.40e-12 63.2
MsG0080048029.01.T01 MTR_8g089575 39.216 102 61 1 51 151 50 151 7.10e-12 63.2
MsG0080048029.01.T01 MTR_3g009190 34.959 123 74 2 45 161 77 199 1.24e-11 62.8
MsG0080048029.01.T01 MTR_3g009190 34.959 123 74 2 45 161 59 181 1.45e-11 62.4
MsG0080048029.01.T01 MTR_6g453040 33.600 125 77 2 45 163 37 161 5.34e-11 60.8
MsG0080048029.01.T01 MTR_6g453040 34.146 123 75 2 45 161 59 181 5.80e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048029.01.T01 AT5G50130 76.974 152 34 1 12 163 1 151 2.46e-78 234
MsG0080048029.01.T01 AT5G50130 76.974 152 34 1 12 163 1 151 2.65e-78 234
MsG0080048029.01.T01 AT5G50130 76.974 152 34 1 12 163 1 151 1.78e-77 234
MsG0080048029.01.T01 AT5G50130 76.974 152 34 1 12 163 1 151 2.01e-77 234
MsG0080048029.01.T01 AT5G50130 76.974 152 34 1 12 163 1 151 2.02e-77 235
MsG0080048029.01.T01 AT4G24050 57.895 152 61 1 12 163 1 149 1.54e-54 174
MsG0080048029.01.T01 AT4G24050 57.895 152 61 1 12 163 1 149 4.41e-54 175
MsG0080048029.01.T01 AT5G50130 75.000 112 28 0 52 163 7 118 8.82e-54 173
MsG0080048029.01.T01 AT5G50130 75.000 112 28 0 52 163 7 118 8.87e-54 173
MsG0080048029.01.T01 AT5G50130 75.000 112 28 0 52 163 7 118 1.01e-53 173
MsG0080048029.01.T01 AT5G50130 75.000 112 28 0 52 163 7 118 1.04e-53 173
MsG0080048029.01.T01 AT1G64590 60.667 150 56 2 12 161 1 147 8.60e-45 151
MsG0080048029.01.T01 AT2G37540 51.266 158 72 2 19 174 7 161 1.37e-43 146
MsG0080048029.01.T01 AT2G37540 50.307 163 76 2 14 174 2 161 7.53e-43 145
MsG0080048029.01.T01 AT4G11410 50.000 152 71 2 24 173 8 156 2.33e-42 143
MsG0080048029.01.T01 AT4G11410 50.000 152 71 2 24 173 8 156 9.35e-42 143
MsG0080048029.01.T01 AT4G23420 50.000 146 70 1 18 163 2 144 1.10e-40 139
MsG0080048029.01.T01 AT4G23430 47.170 159 79 2 18 174 2 157 1.24e-40 140
MsG0080048029.01.T01 AT4G23430 47.170 159 79 2 18 174 2 157 1.34e-40 140
MsG0080048029.01.T01 AT4G23420 50.000 146 70 1 18 163 2 144 1.37e-40 138
MsG0080048029.01.T01 AT4G23420 50.000 146 70 1 18 163 2 144 4.72e-40 138
MsG0080048029.01.T01 AT4G23420 50.000 146 70 1 18 163 2 144 4.72e-40 138
MsG0080048029.01.T01 AT4G23430 49.306 144 70 1 18 161 2 142 6.50e-39 135
MsG0080048029.01.T01 AT4G23430 49.306 144 70 1 18 161 2 142 6.55e-39 135
MsG0080048029.01.T01 AT4G23420 51.449 138 64 1 26 163 27 161 1.41e-38 135
MsG0080048029.01.T01 AT5G02540 51.829 164 74 2 14 175 2 162 4.80e-37 131
MsG0080048029.01.T01 AT5G53090 43.089 123 62 3 43 157 49 171 7.51e-19 83.2
MsG0080048029.01.T01 AT4G09750 34.058 138 84 1 31 161 19 156 2.16e-18 81.3
MsG0080048029.01.T01 AT4G27760 43.902 123 61 3 43 157 50 172 1.42e-17 79.7
MsG0080048029.01.T01 AT5G53100 37.815 119 66 2 47 157 43 161 2.50e-16 75.9
MsG0080048029.01.T01 AT4G27440 36.842 114 66 3 52 161 92 203 2.60e-15 73.2
MsG0080048029.01.T01 AT4G27440 36.842 114 66 3 52 161 92 203 2.60e-15 73.2
MsG0080048029.01.T01 AT5G54190 34.483 116 70 3 52 163 96 209 1.04e-14 71.6
MsG0080048029.01.T01 AT1G03630 36.207 116 68 3 50 161 91 204 1.11e-14 71.6
MsG0080048029.01.T01 AT1G03630 36.207 116 68 3 50 161 89 202 1.13e-14 71.6

Find 34 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGCAGGGCCTAGTGGTTT+TGG 0.230469 contig177end:-4815 MsG0080048029.01.T01:CDS
ACACTTACATGAGAATATTA+AGG 0.272671 contig177end:+4295 None:intergenic
TGCTTGGCAACAAAAGAAAA+AGG 0.274074 contig177end:-4877 MsG0080048029.01.T01:CDS
GAAGGGAACAATAAGGTATT+TGG 0.288975 contig177end:-4843 MsG0080048029.01.T01:CDS
TCAATTGAAGGTGCTACTTC+TGG 0.308401 contig177end:-4522 MsG0080048029.01.T01:intron
AGGTATTTGGCAGGCATTGC+AGG 0.316972 contig177end:-4830 MsG0080048029.01.T01:CDS
TTATGCTGAGGTTATTCTAT+TGG 0.355615 contig177end:-4385 MsG0080048029.01.T01:CDS
ATGAGAGCTGTTAGGTTTGA+AGG 0.360069 contig177end:+4743 None:intergenic
GAAGGTGCTACTTCTGGTAT+TGG 0.364873 contig177end:-4516 MsG0080048029.01.T01:intron
TGGTGCCAGGGACATGAAAA+AGG 0.383783 contig177end:-4445 MsG0080048029.01.T01:CDS
TGAGAGTTGTGGTTGGTGCC+AGG 0.429651 contig177end:-4458 MsG0080048029.01.T01:CDS
CAGCATAAGGACTCTCCTCT+TGG 0.434439 contig177end:+4400 None:intergenic
CATCGATGAAGGGAACAATA+AGG 0.437595 contig177end:-4850 MsG0080048029.01.T01:CDS
ACATTTGCTACAAATTACTT+AGG 0.455021 contig177end:-3918 MsG0080048029.01.T01:CDS
GTTAAGGGCTACAAACTAAA+GGG 0.456879 contig177end:+3815 None:intergenic
CACTTACATGAGAATATTAA+GGG 0.464360 contig177end:+4296 None:intergenic
AATAGAATAACCTCAGCATA+AGG 0.472498 contig177end:+4387 None:intergenic
TACCAGTGATGAGAGCTGTT+AGG 0.489625 contig177end:+4735 None:intergenic
TGAGAGCTGTTAGGTTTGAA+GGG 0.521862 contig177end:+4744 None:intergenic
GGGCTACAAACTAAAGGGTG+TGG 0.543355 contig177end:+3820 None:intergenic
AACCTAACAGCTCTCATCAC+TGG 0.543509 contig177end:-4737 MsG0080048029.01.T01:intron
AGTTGGAGGACACGAATGCT+TGG 0.553276 contig177end:-4893 None:intergenic
GAGAGTTGTGGTTGGTGCCA+GGG 0.563988 contig177end:-4457 MsG0080048029.01.T01:CDS
GAATTTGAGCCAAAACCACT+AGG 0.566141 contig177end:+4806 None:intergenic
GCAGGCATTGCAGGGCCTAG+TGG 0.577354 contig177end:-4821 MsG0080048029.01.T01:CDS
GGTATTTGGCAGGCATTGCA+GGG 0.578276 contig177end:-4829 MsG0080048029.01.T01:CDS
GTGTGGAATTCATATTCTGC+TGG 0.580320 contig177end:+3837 None:intergenic
GAGCTGTTAGGTTTGAAGGG+AGG 0.584163 contig177end:+4747 None:intergenic
GGAACAATAAGGTATTTGGC+AGG 0.598423 contig177end:-4839 MsG0080048029.01.T01:CDS
AAAAGGGAATCATCGATGAA+GGG 0.604378 contig177end:-4860 MsG0080048029.01.T01:CDS
AGGAGTGTACTCTCATAACC+TGG 0.628213 contig177end:-3967 MsG0080048029.01.T01:CDS
AGAGGAGTGAGAGTTGTGGT+TGG 0.631623 contig177end:-4465 MsG0080048029.01.T01:CDS
AGAGGAGAGTCCTTATGCTG+AGG 0.678257 contig177end:-4397 MsG0080048029.01.T01:CDS
ACTTACATGAGAATATTAAG+GGG 0.701725 contig177end:+4297 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGAATATATGTTTTGTTT+TGG - contig177end:4164-4183 MsG0080048029.01.T01:intron 15.0%
!! ATACAATATAATCACAATTG+AGG + contig177end:4587-4606 None:intergenic 20.0%
!! ATTGTGATTATATTGTATTG+TGG - contig177end:4589-4608 MsG0080048029.01.T01:intron 20.0%
! AAGCATAAGAAATGACAATA+AGG + contig177end:4464-4483 None:intergenic 25.0%
! ACACTTACATGAGAATATTA+AGG + contig177end:4422-4441 None:intergenic 25.0%
! ACTTACATGAGAATATTAAG+GGG + contig177end:4420-4439 None:intergenic 25.0%
! CACTTACATGAGAATATTAA+GGG + contig177end:4421-4440 None:intergenic 25.0%
! TGCAAGTATATACATAATAC+TGG + contig177end:4116-4135 None:intergenic 25.0%
!! ACATTTGCTACAAATTACTT+AGG - contig177end:4796-4815 MsG0080048029.01.T01:CDS 25.0%
!! CAAATCTAAAGCTAAAAACT+CGG + contig177end:4397-4416 None:intergenic 25.0%
!! TGTCAATTCAATTTTCTCTT+CGG + contig177end:4779-4798 None:intergenic 25.0%
!! TTTTCATTCTTACATATGAG+AGG - contig177end:4824-4843 MsG0080048029.01.T01:CDS 25.0%
!!! GTTTTGGAATTTTCAATTGA+AGG - contig177end:4180-4199 MsG0080048029.01.T01:intron 25.0%
AATAGAATAACCTCAGCATA+AGG + contig177end:4330-4349 None:intergenic 30.0%
AGTGAAAGAAAAAATCCAAG+AGG - contig177end:4299-4318 MsG0080048029.01.T01:intron 30.0%
TCTGACTACAATATAAAACC+CGG + contig177end:4515-4534 None:intergenic 30.0%
TGATTCATCAGTTGAGAAAA+GGG - contig177end:4485-4504 MsG0080048029.01.T01:CDS 30.0%
TTAGCACAACATGAATTAAC+AGG + contig177end:4685-4704 None:intergenic 30.0%
TTATGCTGAGGTTATTCTAT+TGG - contig177end:4329-4348 MsG0080048029.01.T01:CDS 30.0%
TTGATTCATCAGTTGAGAAA+AGG - contig177end:4484-4503 MsG0080048029.01.T01:CDS 30.0%
AAAAAGGGAATCATCGATGA+AGG - contig177end:3853-3872 MsG0080048029.01.T01:CDS 35.0%
AAAAGGGAATCATCGATGAA+GGG - contig177end:3854-3873 MsG0080048029.01.T01:CDS 35.0%
GCTTGGCAACAAAAGAAAAA+GGG - contig177end:3838-3857 MsG0080048029.01.T01:CDS 35.0%
TAGAGAATCGTGATCAAATG+TGG - contig177end:4552-4571 MsG0080048029.01.T01:intron 35.0%
TGCTTGGCAACAAAAGAAAA+AGG - contig177end:3837-3856 MsG0080048029.01.T01:CDS 35.0%
! GAAGGGAACAATAAGGTATT+TGG - contig177end:3871-3890 MsG0080048029.01.T01:CDS 35.0%
!! TTGTTTTGCAGAAACAATGC+AGG - contig177end:4727-4746 MsG0080048029.01.T01:intron 35.0%
AAAAAGAGGAGTGAGAGTTG+TGG - contig177end:4245-4264 MsG0080048029.01.T01:intron 40.0%
AGATTTGCGACTGCAATCTA+GGG + contig177end:4645-4664 None:intergenic 40.0%
ATGAGAGCTGTTAGGTTTGA+AGG + contig177end:3974-3993 None:intergenic 40.0%
CATCGATGAAGGGAACAATA+AGG - contig177end:3864-3883 MsG0080048029.01.T01:CDS 40.0%
GAATTTGAGCCAAAACCACT+AGG + contig177end:3911-3930 None:intergenic 40.0%
GTGTGGAATTCATATTCTGC+TGG + contig177end:4880-4899 None:intergenic 40.0%
TGAGAGCTGTTAGGTTTGAA+GGG + contig177end:3973-3992 None:intergenic 40.0%
! GCTAGAGTGTTAGCAAAAAG+AGG - contig177end:4231-4250 MsG0080048029.01.T01:intron 40.0%
! GGAACAATAAGGTATTTGGC+AGG - contig177end:3875-3894 MsG0080048029.01.T01:CDS 40.0%
! TTTTCTCTTCGGAGAATTCC+AGG + contig177end:4768-4787 None:intergenic 40.0%
!! TCAATTGAAGGTGCTACTTC+TGG - contig177end:4192-4211 MsG0080048029.01.T01:intron 40.0%
AACCTAACAGCTCTCATCAC+TGG - contig177end:3977-3996 MsG0080048029.01.T01:CDS 45.0%
AGTTGAGAAAAGGGAGATCC+GGG - contig177end:4494-4513 MsG0080048029.01.T01:CDS 45.0%
CAGTTGAGAAAAGGGAGATC+CGG - contig177end:4493-4512 MsG0080048029.01.T01:CDS 45.0%
GAGATTTGCGACTGCAATCT+AGG + contig177end:4646-4665 None:intergenic 45.0%
TACCAGTGATGAGAGCTGTT+AGG + contig177end:3982-4001 None:intergenic 45.0%
! AGGAGTGTACTCTCATAACC+TGG - contig177end:4747-4766 MsG0080048029.01.T01:CDS 45.0%
!! CATAGCCTTTTTCATGTCCC+TGG + contig177end:4277-4296 None:intergenic 45.0%
!! GAAGGTGCTACTTCTGGTAT+TGG - contig177end:4198-4217 MsG0080048029.01.T01:intron 45.0%
AGAGGAGAGTCCTTATGCTG+AGG - contig177end:4317-4336 MsG0080048029.01.T01:CDS 50.0%
AGAGGAGTGAGAGTTGTGGT+TGG - contig177end:4249-4268 MsG0080048029.01.T01:intron 50.0%
CAGCATAAGGACTCTCCTCT+TGG + contig177end:4317-4336 None:intergenic 50.0%
GAGCTGTTAGGTTTGAAGGG+AGG + contig177end:3970-3989 None:intergenic 50.0%
! AGGTATTTGGCAGGCATTGC+AGG - contig177end:3884-3903 MsG0080048029.01.T01:CDS 50.0%
! ATTGCAGGGCCTAGTGGTTT+TGG - contig177end:3899-3918 MsG0080048029.01.T01:CDS 50.0%
! GGTATTTGGCAGGCATTGCA+GGG - contig177end:3885-3904 MsG0080048029.01.T01:CDS 50.0%
! TGGTGCCAGGGACATGAAAA+AGG - contig177end:4269-4288 MsG0080048029.01.T01:intron 50.0%
! TGAGAGTTGTGGTTGGTGCC+AGG - contig177end:4256-4275 MsG0080048029.01.T01:intron 55.0%
!! GAGAGTTGTGGTTGGTGCCA+GGG - contig177end:4257-4276 MsG0080048029.01.T01:intron 55.0%
! GCAGGCATTGCAGGGCCTAG+TGG - contig177end:3893-3912 MsG0080048029.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
contig177end gene 3830 4906 3830 ID=MsG0080048029.01;Name=MsG0080048029.01
contig177end mRNA 3830 4906 3830 ID=MsG0080048029.01.T01;Parent=MsG0080048029.01;Name=MsG0080048029.01.T01;_AED=0.50;_eAED=0.54;_QI=6|1|0.66|1|1|1|3|0|187
contig177end exon 4738 4906 4738 ID=MsG0080048029.01.T01:exon:30977;Parent=MsG0080048029.01.T01
contig177end exon 4303 4534 4303 ID=MsG0080048029.01.T01:exon:30978;Parent=MsG0080048029.01.T01
contig177end exon 3830 3998 3830 ID=MsG0080048029.01.T01:exon:30979;Parent=MsG0080048029.01.T01
contig177end five_prime_UTR 4901 4906 4901 ID=MsG0080048029.01.T01:five_prime_utr;Parent=MsG0080048029.01.T01
contig177end CDS 4738 4900 4738 ID=MsG0080048029.01.T01:cds;Parent=MsG0080048029.01.T01
contig177end CDS 4303 4534 4303 ID=MsG0080048029.01.T01:cds;Parent=MsG0080048029.01.T01
contig177end CDS 3830 3998 3830 ID=MsG0080048029.01.T01:cds;Parent=MsG0080048029.01.T01
Gene Sequence

>MsG0080048029.01.T01

ATGCTTGGCAACAAAAGAAAAAGGGAATCATCGATGAAGGGAACAATAAGGTATTTGGCAGGCATTGCAGGGCCTAGTGGTTTTGGCTCAAATTCAACTGCAGAACAAGTCACTCAGCATTGCTCTTTATTCCTCCCTTCAAACCTAACAGCTCTCATCACTGGTGCTACTTCTGGTATTGGAGCTGAAACAGCTAGAGTGTTAGCAAAAAGAGGAGTGAGAGTTGTGGTTGGTGCCAGGGACATGAAAAAGGCTATGAAAGTGAAAGAAAAAATCCAAGAGGAGAGTCCTTATGCTGAGGTTATTCTATTGGAGATTGATCTTAGCTCTCTTGCTTCTGTACAAAGATTTTGCTCCGAGTTTTTAGCTTTAGATTTGCCCCTTAATATTCTCATAAACAATGCAGGAGTGTACTCTCATAACCTGGAATTCTCCGAAGAGAAAATTGAATTGACATTTGCTACAAATTACTTAGGTACTTTTTTCATTCTTACATATGAGAGGTTGAAAAGCAATGCTAAATCTTTGACAACACCAGCAGAATATGAATTCCACACCCTTTAG

Protein sequence

>MsG0080048029.01.T01

MLGNKRKRESSMKGTIRYLAGIAGPSGFGSNSTAEQVTQHCSLFLPSNLTALITGATSGIGAETARVLAKRGVRVVVGARDMKKAMKVKEKIQEESPYAEVILLEIDLSSLASVQRFCSEFLALDLPLNILINNAGVYSHNLEFSEEKIELTFATNYLGTFFILTYERLKSNAKSLTTPAEYEFHTL*