AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580026593.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026593.01.T01 MTR_5g047050 97.881 236 3 1 1 236 1 234 2.74e-173 477
MsG0580026593.01.T01 MTR_4g109880 47.162 229 108 5 9 236 4 220 1.88e-61 193
MsG0580026593.01.T01 MTR_3g072390 35.366 164 91 5 74 236 73 222 1.34e-20 87.4
MsG0580026593.01.T01 MTR_2g055760 32.143 140 84 3 79 216 16 146 4.11e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580026593.01.T01 AT1G69080 42.276 246 107 6 1 236 1 221 7.21e-59 186
MsG0580026593.01.T01 AT2G03720 52.326 172 74 6 67 236 65 230 3.61e-53 172
MsG0580026593.01.T01 AT2G03720 52.071 169 73 6 70 236 1 163 1.16e-51 166
MsG0580026593.01.T01 AT1G69080 39.837 246 99 7 1 236 1 207 6.16e-50 163
MsG0580026593.01.T01 AT5G17390 47.977 173 79 5 68 236 118 283 5.59e-48 160
MsG0580026593.01.T01 AT3G03290 45.665 173 83 5 68 236 107 272 2.39e-44 150
MsG0580026593.01.T01 AT1G44760 36.257 171 88 5 66 236 62 211 1.37e-26 103
MsG0580026593.01.T01 AT1G44760 36.257 171 88 5 66 236 133 282 4.95e-26 103
MsG0580026593.01.T01 AT1G48960 30.488 164 99 6 67 230 5 153 1.69e-14 70.9
MsG0580026593.01.T01 AT4G13450 26.923 208 111 7 66 236 14 217 9.65e-14 68.9

Find 43 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCCATACAATGTGCATTT+TGG 0.179754 5:-40154607 None:intergenic
TGATCAATATAGTTATTCTT+TGG 0.264826 5:+40154709 MsG0580026593.01.T01:CDS
AGAAGAAAGAGTAAGAAAAT+TGG 0.268808 5:+40154638 MsG0580026593.01.T01:CDS
AAGACAAGGCGTGGCACTTT+TGG 0.275641 5:+40154493 MsG0580026593.01.T01:CDS
TCATGCTTGGCTTGTATAGA+TGG 0.307653 5:-40153544 None:intergenic
AAACCTTGTTTAGAATTAGA+AGG 0.359582 5:-40153784 None:intergenic
TGCCCATTGCAAAGCACCTT+TGG 0.367782 5:-40153707 None:intergenic
TACTCACACTGTTCAGAATC+AGG 0.397968 5:+40153732 MsG0580026593.01.T01:CDS
TATCCAAAATGCACATTGTA+TGG 0.401167 5:+40154604 MsG0580026593.01.T01:CDS
AAGAGAGGAGCAAGGTTACC+AGG 0.459111 5:+40153478 MsG0580026593.01.T01:CDS
ATCACTTATAGAAATCTTGA+AGG 0.463007 5:-40154744 None:intergenic
TATAGTTATTCTTTGGATGT+TGG 0.463118 5:+40154716 MsG0580026593.01.T01:CDS
AGCCAAAGGTGCTTTGCAAT+GGG 0.470225 5:+40153705 MsG0580026593.01.T01:CDS
AAGCCAAAGGTGCTTTGCAA+TGG 0.482336 5:+40153704 MsG0580026593.01.T01:CDS
AGAAAGAGTAAGAAAATTGG+AGG 0.492506 5:+40154641 MsG0580026593.01.T01:CDS
TGATGATGAAGCTTCTAGTA+AGG 0.508679 5:+40153906 MsG0580026593.01.T01:CDS
AGTAACAGAAGGGAAAGAAA+AGG 0.525585 5:+40154445 MsG0580026593.01.T01:CDS
AACAAGGGTCAGTAGTTAGT+AGG 0.536437 5:+40153650 MsG0580026593.01.T01:CDS
GTAACAGAAGGGAAAGAAAA+GGG 0.538384 5:+40154446 MsG0580026593.01.T01:CDS
TGGGCTGGCAATAGAGTCAC+TGG 0.549351 5:+40154563 MsG0580026593.01.T01:CDS
AAGCCTTCTAATTCTAAACA+AGG 0.553728 5:+40153781 MsG0580026593.01.T01:CDS
TCGAATTAGGCCACATGCGA+GGG 0.562880 5:+40153513 MsG0580026593.01.T01:CDS
TTTGACGATGGTGTGAAACA+AGG 0.566437 5:+40153634 MsG0580026593.01.T01:CDS
GGCGAACGCACCCTCGCATG+TGG 0.567866 5:-40153523 None:intergenic
GAAGCAAGTTCATAAGCCCT+TGG 0.570979 5:-40153937 None:intergenic
TTCCAAAACATGGGCAAGAG+AGG 0.576269 5:+40153463 None:intergenic
CGTCTTCTAATGATGTGGGC+TGG 0.577564 5:+40154548 MsG0580026593.01.T01:CDS
CATTAGAAGACGCCATGTTG+TGG 0.577627 5:-40154537 None:intergenic
ATTGAGATTGCAGTAACAGA+AGG 0.585136 5:+40154434 MsG0580026593.01.T01:CDS
CATGGCGTCTTCTAATGATG+TGG 0.588320 5:+40154543 MsG0580026593.01.T01:CDS
GAAAGAGTAAGAAAATTGGA+GGG 0.591657 5:+40154642 MsG0580026593.01.T01:CDS
ATCGAATTAGGCCACATGCG+AGG 0.593025 5:+40153512 MsG0580026593.01.T01:CDS
CAATAGAGTCACTGGTGGAG+TGG 0.594867 5:+40154571 MsG0580026593.01.T01:CDS
TTGACGATGGTGTGAAACAA+GGG 0.607583 5:+40153635 MsG0580026593.01.T01:CDS
TTGAGATTGCAGTAACAGAA+GGG 0.610661 5:+40154435 MsG0580026593.01.T01:CDS
GAATTCATGCAGTGTTTGCG+AGG 0.613944 5:+40153606 MsG0580026593.01.T01:CDS
CTAGTAAGGAGACAGATCCA+AGG 0.630181 5:+40153920 MsG0580026593.01.T01:CDS
ATGGCGTCTTCTAATGATGT+GGG 0.632260 5:+40154544 MsG0580026593.01.T01:CDS
TAGTAAGGAGACAGATCCAA+GGG 0.638911 5:+40153921 MsG0580026593.01.T01:CDS
GCTGGCAATAGAGTCACTGG+TGG 0.677998 5:+40154566 MsG0580026593.01.T01:CDS
TGCGAGGAGAAATTTGACGA+TGG 0.693381 5:+40153622 MsG0580026593.01.T01:CDS
ACATGGGCAAGAGAGGAGCA+AGG 0.696922 5:+40153470 None:intergenic
TTGTAATGTGAAGATGCCTG+AGG 0.775212 5:+40153975 MsG0580026593.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CAAATTTAAAATTATTTCAA+AGG - Chr5:40154205-40154224 None:intergenic 10.0%
!! ATTATCATACAAAGAATTTA+AGG - Chr5:40154272-40154291 None:intergenic 15.0%
!! CATTATAATGTAAATAAACA+AGG + Chr5:40154299-40154318 MsG0580026593.01.T01:intron 15.0%
!! AAAACATACAATGATATCAT+TGG - Chr5:40153816-40153835 None:intergenic 20.0%
!! AAGGTTAAATGTTAAAATCT+CGG - Chr5:40154186-40154205 None:intergenic 20.0%
!! TGATCAATATAGTTATTCTT+TGG + Chr5:40154709-40154728 MsG0580026593.01.T01:CDS 20.0%
!!! ATTTTAACACTGAAAAATAG+AGG + Chr5:40154072-40154091 MsG0580026593.01.T01:intron 20.0%
! AAATGTTGAGACTGTATATA+TGG + Chr5:40154396-40154415 MsG0580026593.01.T01:intron 25.0%
! AGAAGAAAGAGTAAGAAAAT+TGG + Chr5:40154638-40154657 MsG0580026593.01.T01:CDS 25.0%
! ATCACTTATAGAAATCTTGA+AGG - Chr5:40154747-40154766 None:intergenic 25.0%
! CAAGTATGATTCATTCAATA+TGG + Chr5:40153876-40153895 MsG0580026593.01.T01:intron 25.0%
! TATAGTTATTCTTTGGATGT+TGG + Chr5:40154716-40154735 MsG0580026593.01.T01:CDS 25.0%
!! AAACCTTGTTTAGAATTAGA+AGG - Chr5:40153787-40153806 None:intergenic 25.0%
!! TATATTGTCACTTACTTACA+TGG - Chr5:40154349-40154368 None:intergenic 25.0%
AAGCCTTCTAATTCTAAACA+AGG + Chr5:40153781-40153800 MsG0580026593.01.T01:CDS 30.0%
AATCTCAATTTGTACCTATC+AGG - Chr5:40154423-40154442 None:intergenic 30.0%
AGAAAGAGTAAGAAAATTGG+AGG + Chr5:40154641-40154660 MsG0580026593.01.T01:CDS 30.0%
GAAAGAGTAAGAAAATTGGA+GGG + Chr5:40154642-40154661 MsG0580026593.01.T01:CDS 30.0%
TATCCAAAATGCACATTGTA+TGG + Chr5:40154604-40154623 MsG0580026593.01.T01:CDS 30.0%
TTCATCTTGTACCATTGTTT+AGG + Chr5:40154121-40154140 MsG0580026593.01.T01:intron 30.0%
! ACTAAGCGTCATAAGATTTT+TGG + Chr5:40154677-40154696 MsG0580026593.01.T01:CDS 30.0%
! GTATGTTTTCTCTTCAACTT+TGG + Chr5:40153827-40153846 MsG0580026593.01.T01:intron 30.0%
! GTTTTTGCTTGAATCGAATT+AGG + Chr5:40153500-40153519 MsG0580026593.01.T01:CDS 30.0%
!! ATCATCACTTTTATCATGCT+TGG - Chr5:40153560-40153579 None:intergenic 30.0%
AACAATGGTACAAGATGAAG+TGG - Chr5:40154120-40154139 None:intergenic 35.0%
ACTGTATATATGGTCCTGAT+AGG + Chr5:40154406-40154425 MsG0580026593.01.T01:intron 35.0%
AGAAAAGGGACCAAAAATAG+TGG + Chr5:40154460-40154479 MsG0580026593.01.T01:CDS 35.0%
AGTAACAGAAGGGAAAGAAA+AGG + Chr5:40154445-40154464 MsG0580026593.01.T01:CDS 35.0%
ATTCGATTCAAGCAAAAACC+TGG - Chr5:40153499-40153518 None:intergenic 35.0%
ATTGAGATTGCAGTAACAGA+AGG + Chr5:40154434-40154453 MsG0580026593.01.T01:CDS 35.0%
GTAACAGAAGGGAAAGAAAA+GGG + Chr5:40154446-40154465 MsG0580026593.01.T01:CDS 35.0%
TAAGATGATATCAGAGTCTC+AGG - Chr5:40154012-40154031 None:intergenic 35.0%
TTGAGATTGCAGTAACAGAA+GGG + Chr5:40154435-40154454 MsG0580026593.01.T01:CDS 35.0%
! TGATGATGAAGCTTCTAGTA+AGG + Chr5:40153906-40153925 MsG0580026593.01.T01:CDS 35.0%
! TTGCCATACAATGTGCATTT+TGG - Chr5:40154610-40154629 None:intergenic 35.0%
! TTGTCACTTACTTACATGGA+TGG - Chr5:40154345-40154364 None:intergenic 35.0%
! TTTGCTTCCTCCACTATTTT+TGG - Chr5:40154473-40154492 None:intergenic 35.0%
!!! GATTCTAGTTTTGAAGCCAA+AGG + Chr5:40153691-40153710 MsG0580026593.01.T01:CDS 35.0%
!!! GTCATAAGATTTTTGGCTCT+TGG + Chr5:40154684-40154703 MsG0580026593.01.T01:CDS 35.0%
AAAGGGACCAAAAATAGTGG+AGG + Chr5:40154463-40154482 MsG0580026593.01.T01:CDS 40.0%
AACAAGGGTCAGTAGTTAGT+AGG + Chr5:40153650-40153669 MsG0580026593.01.T01:CDS 40.0%
AGAAAAAGAGGTCCACAACA+TGG + Chr5:40154525-40154544 MsG0580026593.01.T01:CDS 40.0%
ATGGCGTCTTCTAATGATGT+GGG + Chr5:40154544-40154563 MsG0580026593.01.T01:CDS 40.0%
TACTCACACTGTTCAGAATC+AGG + Chr5:40153732-40153751 MsG0580026593.01.T01:CDS 40.0%
TAGTAAGGAGACAGATCCAA+GGG + Chr5:40153921-40153940 MsG0580026593.01.T01:CDS 40.0%
TCGCGATACAACCTAAACAA+TGG - Chr5:40154135-40154154 None:intergenic 40.0%
TTGACGATGGTGTGAAACAA+GGG + Chr5:40153635-40153654 MsG0580026593.01.T01:CDS 40.0%
TTGTAATGTGAAGATGCCTG+AGG + Chr5:40153975-40153994 MsG0580026593.01.T01:CDS 40.0%
TTTGACGATGGTGTGAAACA+AGG + Chr5:40153634-40153653 MsG0580026593.01.T01:CDS 40.0%
!! TCATGCTTGGCTTGTATAGA+TGG - Chr5:40153547-40153566 None:intergenic 40.0%
CATGGCGTCTTCTAATGATG+TGG + Chr5:40154543-40154562 MsG0580026593.01.T01:CDS 45.0%
CATTAGAAGACGCCATGTTG+TGG - Chr5:40154540-40154559 None:intergenic 45.0%
CTAGTAAGGAGACAGATCCA+AGG + Chr5:40153920-40153939 MsG0580026593.01.T01:CDS 45.0%
GAAGCAAGTTCATAAGCCCT+TGG - Chr5:40153940-40153959 None:intergenic 45.0%
GAATTCATGCAGTGTTTGCG+AGG + Chr5:40153606-40153625 MsG0580026593.01.T01:CDS 45.0%
GTGGAGGAAGCAAAAAGACA+AGG + Chr5:40154479-40154498 MsG0580026593.01.T01:CDS 45.0%
TGCGAGGAGAAATTTGACGA+TGG + Chr5:40153622-40153641 MsG0580026593.01.T01:CDS 45.0%
!! AAGCCAAAGGTGCTTTGCAA+TGG + Chr5:40153704-40153723 MsG0580026593.01.T01:CDS 45.0%
!! AGCCAAAGGTGCTTTGCAAT+GGG + Chr5:40153705-40153724 MsG0580026593.01.T01:CDS 45.0%
!!! TGGTTTTGGGGCAGAAAAAG+AGG + Chr5:40154513-40154532 MsG0580026593.01.T01:CDS 45.0%
AAGAGAGGAGCAAGGTTACC+AGG + Chr5:40153478-40153497 MsG0580026593.01.T01:CDS 50.0%
ATCGAATTAGGCCACATGCG+AGG + Chr5:40153512-40153531 MsG0580026593.01.T01:CDS 50.0%
CAATAGAGTCACTGGTGGAG+TGG + Chr5:40154571-40154590 MsG0580026593.01.T01:CDS 50.0%
CGTCTTCTAATGATGTGGGC+TGG + Chr5:40154548-40154567 MsG0580026593.01.T01:CDS 50.0%
GGAAGCAAAAAGACAAGGCG+TGG + Chr5:40154484-40154503 MsG0580026593.01.T01:CDS 50.0%
TCAGGAGCTAAACTCACCTC+AGG - Chr5:40153994-40154013 None:intergenic 50.0%
TCGAATTAGGCCACATGCGA+GGG + Chr5:40153513-40153532 MsG0580026593.01.T01:CDS 50.0%
! AAGACAAGGCGTGGCACTTT+TGG + Chr5:40154493-40154512 MsG0580026593.01.T01:CDS 50.0%
! TGCCCATTGCAAAGCACCTT+TGG - Chr5:40153710-40153729 None:intergenic 50.0%
!!! AGGCGTGGCACTTTTGGTTT+TGG + Chr5:40154499-40154518 MsG0580026593.01.T01:CDS 50.0%
!!! GCGTGGCACTTTTGGTTTTG+GGG + Chr5:40154501-40154520 MsG0580026593.01.T01:CDS 50.0%
!!! GGCGTGGCACTTTTGGTTTT+GGG + Chr5:40154500-40154519 MsG0580026593.01.T01:CDS 50.0%
GCTGGCAATAGAGTCACTGG+TGG + Chr5:40154566-40154585 MsG0580026593.01.T01:CDS 55.0%
!! TGGGCTGGCAATAGAGTCAC+TGG + Chr5:40154563-40154582 MsG0580026593.01.T01:CDS 55.0%
GGCGAACGCACCCTCGCATG+TGG - Chr5:40153526-40153545 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 40153472 40154769 40153472 ID=MsG0580026593.01;Name=MsG0580026593.01
Chr5 mRNA 40153472 40154769 40153472 ID=MsG0580026593.01.T01;Parent=MsG0580026593.01;Name=MsG0580026593.01.T01;_AED=0.28;_eAED=0.35;_QI=0|0|0|1|1|1|3|0|255
Chr5 exon 40153472 40153802 40153472 ID=MsG0580026593.01.T01:exon:37196;Parent=MsG0580026593.01.T01
Chr5 exon 40153902 40153996 40153902 ID=MsG0580026593.01.T01:exon:37197;Parent=MsG0580026593.01.T01
Chr5 exon 40154428 40154769 40154428 ID=MsG0580026593.01.T01:exon:37198;Parent=MsG0580026593.01.T01
Chr5 CDS 40153472 40153802 40153472 ID=MsG0580026593.01.T01:cds;Parent=MsG0580026593.01.T01
Chr5 CDS 40153902 40153996 40153902 ID=MsG0580026593.01.T01:cds;Parent=MsG0580026593.01.T01
Chr5 CDS 40154428 40154769 40154428 ID=MsG0580026593.01.T01:cds;Parent=MsG0580026593.01.T01
Gene Sequence

>MsG0580026593.01.T01

ATGGGCAAGAGAGGAGCAAGGTTACCAGGTTTTTGCTTGAATCGAATTAGGCCACATGCGAGGGTGCGTTCGCCATCTATACAAGCCAAGCATGATAAAAGTGATGATGCTAAAATTGATCAGAAAATTACTGAGAATTCATGCAGTGTTTGCGAGGAGAAATTTGACGATGGTGTGAAACAAGGGTCAGTAGTTAGTAGGAAGATTATGATTGTGATTGATTCTAGTTTTGAAGCCAAAGGTGCTTTGCAATGGGCACTTACTCACACTGTTCAGAATCAGGATACTATAGTTCTTCTTCATGTCATGAAGCCTTCTAATTCTAAACAAGAAACTGATGATGAAGCTTCTAGTAAGGAGACAGATCCAAGGGCTTATGAACTTGCTTCATCTTTCAAGAATATTTGTAATGTGAAGATGCCTGAGGTACAAATTGAGATTGCAGTAACAGAAGGGAAAGAAAAGGGACCAAAAATAGTGGAGGAAGCAAAAAGACAAGGCGTGGCACTTTTGGTTTTGGGGCAGAAAAAGAGGTCCACAACATGGCGTCTTCTAATGATGTGGGCTGGCAATAGAGTCACTGGTGGAGTGGTAGAGTATTGTATCCAAAATGCACATTGTATGGCAATTGCTGTGAGAAGAAAGAGTAAGAAAATTGGAGGGTATATGATTACTACTAAGCGTCATAAGATTTTTGGCTCTTGGCTTGATCAATATAGTTATTCTTTGGATGTTGGTGTCACCTTCAAGATTTCTATAAGTGATTGA

Protein sequence

>MsG0580026593.01.T01

MGKRGARLPGFCLNRIRPHARVRSPSIQAKHDKSDDAKIDQKITENSCSVCEEKFDDGVKQGSVVSRKIMIVIDSSFEAKGALQWALTHTVQNQDTIVLLHVMKPSNSKQETDDEASSKETDPRAYELASSFKNICNVKMPEVQIEIAVTEGKEKGPKIVEEAKRQGVALLVLGQKKRSTTWRLLMMWAGNRVTGGVVEYCIQNAHCMAIAVRRKSKKIGGYMITTKRHKIFGSWLDQYSYSLDVGVTFKISISD*