AlfalfaGEDB | MsG0580026593.01

M. sativa cultivar ZhongmuNo.1 / MsG0580026593.01

KEGG: -
GO: GO:0006950
PFAM: PF00582

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026593.01.T01	XP_003614248.1	97.881	236	3	1	1	236	1	234	2.27E-169	477

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026593.01.T01	G7JY47	97.881	236	3	1	1	236	1	234	1.08e-169	477

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047814.01	MsG0580026593.01	0.843867	1.075709e-58	1.705999e-55
MsG0080047974.01	MsG0580026593.01	0.830950	2.200076e-55	2.353166e-52
MsG0080048029.01	MsG0580026593.01	0.809159	2.172164e-50	1.265898e-47
MsG0080048291.01	MsG0580026593.01	0.818725	1.687069e-52	1.272874e-49
MsG0080048856.01	MsG0580026593.01	0.832933	7.124062e-56	8.081115e-53
MsG0080049140.01	MsG0580026593.01	0.846021	2.820872e-59	4.790698e-56
MsG0180000024.01	MsG0580026593.01	0.801533	8.611245e-49	4.122280e-46
MsG0180000139.01	MsG0580026593.01	0.801117	1.047218e-48	4.961031e-46
MsG0180000145.01	MsG0580026593.01	0.812282	4.584426e-51	2.902831e-48
MsG0180000295.01	MsG0580026593.01	0.821683	3.543781e-53	2.903033e-50

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026593.01.T01	MTR_5g047050	97.881	236	3	1	1	236	1	234	2.74e-173	477
MsG0580026593.01.T01	MTR_4g109880	47.162	229	108	5	9	236	4	220	1.88e-61	193
MsG0580026593.01.T01	MTR_3g072390	35.366	164	91	5	74	236	73	222	1.34e-20	87.4
MsG0580026593.01.T01	MTR_2g055760	32.143	140	84	3	79	216	16	146	4.11e-14	69.3

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580026593.01.T01	AT1G69080	42.276	246	107	6	1	236	1	221	7.21e-59	186
MsG0580026593.01.T01	AT2G03720	52.326	172	74	6	67	236	65	230	3.61e-53	172
MsG0580026593.01.T01	AT2G03720	52.071	169	73	6	70	236	1	163	1.16e-51	166
MsG0580026593.01.T01	AT1G69080	39.837	246	99	7	1	236	1	207	6.16e-50	163
MsG0580026593.01.T01	AT5G17390	47.977	173	79	5	68	236	118	283	5.59e-48	160
MsG0580026593.01.T01	AT3G03290	45.665	173	83	5	68	236	107	272	2.39e-44	150
MsG0580026593.01.T01	AT1G44760	36.257	171	88	5	66	236	62	211	1.37e-26	103
MsG0580026593.01.T01	AT1G44760	36.257	171	88	5	66	236	133	282	4.95e-26	103
MsG0580026593.01.T01	AT1G48960	30.488	164	99	6	67	230	5	153	1.69e-14	70.9
MsG0580026593.01.T01	AT4G13450	26.923	208	111	7	66	236	14	217	9.65e-14	68.9

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Find 43 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCTTGTTTAGAATTAGA+AGG	0.359582	5:-40153784	None:intergenic
AACAAGGGTCAGTAGTTAGT+AGG	0.536437	5:+40153650	MsG0580026593.01.T01:CDS
AAGACAAGGCGTGGCACTTT+TGG	0.275641	5:+40154493	MsG0580026593.01.T01:CDS
AAGAGAGGAGCAAGGTTACC+AGG	0.459111	5:+40153478	MsG0580026593.01.T01:CDS
AAGCCAAAGGTGCTTTGCAA+TGG	0.482336	5:+40153704	MsG0580026593.01.T01:CDS
AAGCCTTCTAATTCTAAACA+AGG	0.553728	5:+40153781	MsG0580026593.01.T01:CDS
ACATGGGCAAGAGAGGAGCA+AGG	0.696922	5:+40153470	None:intergenic
AGAAAGAGTAAGAAAATTGG+AGG	0.492506	5:+40154641	MsG0580026593.01.T01:CDS
AGAAGAAAGAGTAAGAAAAT+TGG	0.268808	5:+40154638	MsG0580026593.01.T01:CDS
AGCCAAAGGTGCTTTGCAAT+GGG	0.470225	5:+40153705	MsG0580026593.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGCCTTCTAATTCTAAACA+AGG	+	Chr5:40153781-40153800	MsG0580026593.01.T01:CDS	30.0%
AATCTCAATTTGTACCTATC+AGG	-	Chr5:40154423-40154442	None:intergenic	30.0%
AGAAAGAGTAAGAAAATTGG+AGG	+	Chr5:40154641-40154660	MsG0580026593.01.T01:CDS	30.0%
GAAAGAGTAAGAAAATTGGA+GGG	+	Chr5:40154642-40154661	MsG0580026593.01.T01:CDS	30.0%
TATCCAAAATGCACATTGTA+TGG	+	Chr5:40154604-40154623	MsG0580026593.01.T01:CDS	30.0%
TTCATCTTGTACCATTGTTT+AGG	+	Chr5:40154121-40154140	MsG0580026593.01.T01:intron	30.0%
AACAATGGTACAAGATGAAG+TGG	-	Chr5:40154120-40154139	None:intergenic	35.0%
ACTGTATATATGGTCCTGAT+AGG	+	Chr5:40154406-40154425	MsG0580026593.01.T01:intron	35.0%
AGAAAAGGGACCAAAAATAG+TGG	+	Chr5:40154460-40154479	MsG0580026593.01.T01:CDS	35.0%
AGTAACAGAAGGGAAAGAAA+AGG	+	Chr5:40154445-40154464	MsG0580026593.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	40153472	40154769	40153472	ID=MsG0580026593.01;Name=MsG0580026593.01
Chr5	mRNA	40153472	40154769	40153472	ID=MsG0580026593.01.T01;Parent=MsG0580026593.01;Name=MsG0580026593.01.T01;_AED=0.28;_eAED=0.35;_QI=0\|0\|0\|1\|1\|1\|3\|0\|255
Chr5	exon	40153472	40153802	40153472	ID=MsG0580026593.01.T01:exon:37196;Parent=MsG0580026593.01.T01
Chr5	exon	40153902	40153996	40153902	ID=MsG0580026593.01.T01:exon:37197;Parent=MsG0580026593.01.T01
Chr5	exon	40154428	40154769	40154428	ID=MsG0580026593.01.T01:exon:37198;Parent=MsG0580026593.01.T01
Chr5	CDS	40153472	40153802	40153472	ID=MsG0580026593.01.T01:cds;Parent=MsG0580026593.01.T01
Chr5	CDS	40153902	40153996	40153902	ID=MsG0580026593.01.T01:cds;Parent=MsG0580026593.01.T01
Chr5	CDS	40154428	40154769	40154428	ID=MsG0580026593.01.T01:cds;Parent=MsG0580026593.01.T01

Gene Sequence

>MsG0580026593.01.T01

ATGGGCAAGAGAGGAGCAAGGTTACCAGGTTTTTGCTTGAATCGAATTAGGCCACATGCGAGGGTGCGTTCGCCATCTATACAAGCCAAGCATGATAAAAGTGATGATGCTAAAATTGATCAGAAAATTACTGAGAATTCATGCAGTGTTTGCGAGGAGAAATTTGACGATGGTGTGAAACAAGGGTCAGTAGTTAGTAGGAAGATTATGATTGTGATTGATTCTAGTTTTGAAGCCAAAGGTGCTTTGCAATGGGCACTTACTCACACTGTTCAGAATCAGGATACTATAGTTCTTCTTCATGTCATGAAGCCTTCTAATTCTAAACAAGAAACTGATGATGAAGCTTCTAGTAAGGAGACAGATCCAAGGGCTTATGAACTTGCTTCATCTTTCAAGAATATTTGTAATGTGAAGATGCCTGAGGTACAAATTGAGATTGCAGTAACAGAAGGGAAAGAAAAGGGACCAAAAATAGTGGAGGAAGCAAAAAGACAAGGCGTGGCACTTTTGGTTTTGGGGCAGAAAAAGAGGTCCACAACATGGCGTCTTCTAATGATGTGGGCTGGCAATAGAGTCACTGGTGGAGTGGTAGAGTATTGTATCCAAAATGCACATTGTATGGCAATTGCTGTGAGAAGAAAGAGTAAGAAAATTGGAGGGTATATGATTACTACTAAGCGTCATAAGATTTTTGGCTCTTGGCTTGATCAATATAGTTATTCTTTGGATGTTGGTGTCACCTTCAAGATTTCTATAAGTGATTGA

Protein sequence

>MsG0580026593.01.T01

MGKRGARLPGFCLNRIRPHARVRSPSIQAKHDKSDDAKIDQKITENSCSVCEEKFDDGVKQGSVVSRKIMIVIDSSFEAKGALQWALTHTVQNQDTIVLLHVMKPSNSKQETDDEASSKETDPRAYELASSFKNICNVKMPEVQIEIAVTEGKEKGPKIVEEAKRQGVALLVLGQKKRSTTWRLLMMWAGNRVTGGVVEYCIQNAHCMAIAVRRKSKKIGGYMITTKRHKIFGSWLDQYSYSLDVGVTFKISISD*