AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002183.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002183.01.T01 MTR_1g049170 88.584 219 25 0 1 219 172 390 6.24e-146 412
MsG0180002183.01.T01 MTR_3g092000 70.455 220 61 3 1 218 187 404 9.85e-105 308
MsG0180002183.01.T01 MTR_3g091990 63.256 215 73 3 3 214 176 387 2.51e-93 279
MsG0180002183.01.T01 MTR_2g047180 52.055 219 89 6 3 214 158 367 1.43e-67 212
MsG0180002183.01.T01 MTR_3g030080 51.376 218 92 7 3 214 164 373 5.63e-65 205
MsG0180002183.01.T01 MTR_3g029650 53.636 220 95 6 5 219 150 367 1.63e-63 210
MsG0180002183.01.T01 MTR_3g029650 42.478 226 113 7 1 215 533 752 1.70e-42 152
MsG0180002183.01.T01 MTR_1g016540 56.593 182 65 6 45 214 1 180 1.92e-58 182
MsG0180002183.01.T01 MTR_3g030480 50.691 217 89 7 1 214 154 355 2.28e-58 188
MsG0180002183.01.T01 MTR_3g030440 50.691 217 89 7 1 214 155 356 2.81e-58 187
MsG0180002183.01.T01 MTR_6g018315 50.909 220 95 7 3 212 149 365 8.17e-58 187
MsG0180002183.01.T01 MTR_6g018360 50.909 220 95 7 3 212 164 380 1.41e-57 187
MsG0180002183.01.T01 MTR_3g030380 48.848 217 94 6 1 214 156 358 2.14e-56 183
MsG0180002183.01.T01 MTR_3g030050 43.028 251 103 4 1 212 171 420 3.74e-55 181
MsG0180002183.01.T01 MTR_3g030360 47.907 215 77 7 3 214 170 352 1.74e-54 178
MsG0180002183.01.T01 MTR_6g018390 48.341 211 97 6 4 212 165 365 2.77e-52 172
MsG0180002183.01.T01 MTR_1g016570 46.759 216 94 6 3 214 163 361 3.48e-52 172
MsG0180002183.01.T01 MTR_3g029640 45.370 216 107 6 5 216 159 367 7.31e-52 171
MsG0180002183.01.T01 MTR_3g029750 46.818 220 100 7 3 215 179 388 4.88e-51 170
MsG0180002183.01.T01 MTR_6g018400 47.418 213 99 6 3 212 166 368 5.13e-51 169
MsG0180002183.01.T01 MTR_3g037710 42.723 213 108 2 14 212 50 262 1.15e-50 166
MsG0180002183.01.T01 MTR_1g026850 46.047 215 108 5 4 213 153 364 4.10e-50 167
MsG0180002183.01.T01 MTR_3g029620 44.954 218 106 6 6 215 164 375 7.55e-50 166
MsG0180002183.01.T01 MTR_3g029670 46.364 220 101 7 3 215 179 388 1.21e-49 166
MsG0180002183.01.T01 MTR_3g030355 44.889 225 109 6 3 215 175 396 2.49e-49 166
MsG0180002183.01.T01 MTR_6g027100 45.833 216 99 7 3 212 172 375 3.86e-49 166
MsG0180002183.01.T01 MTR_1g007260 46.445 211 99 8 4 208 181 383 1.04e-48 164
MsG0180002183.01.T01 MTR_6g027040 45.833 216 99 7 3 212 172 375 3.08e-48 162
MsG0180002183.01.T01 MTR_3g030560 42.601 223 114 6 2 218 164 378 4.64e-47 159
MsG0180002183.01.T01 MTR_3g029920 43.290 231 105 12 1 218 167 384 3.33e-44 152
MsG0180002183.01.T01 MTR_3g029900 45.024 211 99 5 3 212 263 457 3.78e-44 154
MsG0180002183.01.T01 MTR_3g058460 42.308 208 107 5 17 212 169 375 1.68e-43 150
MsG0180002183.01.T01 MTR_3g047980 42.512 207 91 6 12 212 175 359 4.96e-43 149
MsG0180002183.01.T01 MTR_3g029800 40.758 211 108 5 13 216 159 359 6.75e-43 148
MsG0180002183.01.T01 MTR_1g007250 50.000 166 72 6 45 205 1 160 2.66e-41 138
MsG0180002183.01.T01 MTR_3g029840 44.601 213 98 6 3 214 163 356 3.97e-41 143
MsG0180002183.01.T01 MTR_3g029820 45.556 180 76 8 49 218 165 332 3.07e-40 140
MsG0180002183.01.T01 MTR_5g078450 53.623 138 42 3 79 214 2 119 1.22e-39 132
MsG0180002183.01.T01 MTR_5g042230 46.591 176 89 4 31 201 13 188 5.12e-39 134
MsG0180002183.01.T01 MTR_3g029950 43.750 224 96 8 3 214 163 368 1.22e-38 137
MsG0180002183.01.T01 MTR_8g035995 68.132 91 28 1 55 144 2 92 1.06e-34 121
MsG0180002183.01.T01 MTR_6g018370 44.304 158 83 3 1 153 102 259 2.04e-34 123
MsG0180002183.01.T01 MTR_1g007220 38.660 194 87 5 15 205 3 167 1.43e-33 119
MsG0180002183.01.T01 MTR_4g022880 48.120 133 62 3 87 212 39 171 1.19e-32 117
MsG0180002183.01.T01 MTR_7g005990 39.336 211 97 6 13 215 172 359 3.25e-31 117
MsG0180002183.01.T01 MTR_3g029870 45.562 169 65 6 58 214 182 335 7.98e-29 110
MsG0180002183.01.T01 MTR_1g007200 46.154 104 42 6 99 198 1 94 5.87e-16 71.6
MsG0180002183.01.T01 MTR_3g029850 39.744 78 44 2 135 212 42 116 1.82e-13 65.1
MsG0180002183.01.T01 MTR_8g074610 28.571 203 119 8 25 212 204 395 2.72e-13 68.6
MsG0180002183.01.T01 MTR_7g012990 48.052 77 36 3 36 110 79 153 2.55e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002183.01.T01 AT2G17030 34.322 236 128 7 1 212 158 390 7.77e-28 109
MsG0180002183.01.T01 AT2G17036 32.627 236 125 12 1 212 159 384 2.92e-22 94.0
MsG0180002183.01.T01 AT2G17036 32.627 236 125 12 1 212 158 383 3.02e-22 94.0
MsG0180002183.01.T01 AT5G24040 24.299 214 150 7 9 213 75 285 5.98e-13 66.6
MsG0180002183.01.T01 AT5G24040 24.299 214 150 7 9 213 160 370 1.49e-12 66.2
MsG0180002183.01.T01 AT5G24040 23.529 187 133 5 36 213 28 213 5.86e-11 60.5

Find 46 sgRNAs with CRISPR-Local

Find 60 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTATGACTCCCATTGTTT+TGG 0.184600 1:-34390612 MsG0180002183.01.T01:CDS
CTGACTACTTCAACTTCTTC+TGG 0.327644 1:-34390326 MsG0180002183.01.T01:CDS
ACAACACTGGATGACCGTTT+TGG 0.357465 1:+34390850 None:intergenic
GTCGAAGAGCTTAAGGGATA+AGG 0.404239 1:-34390523 MsG0180002183.01.T01:CDS
ATTGGTAGGACCGTTGCGTT+TGG 0.412281 1:-34390729 MsG0180002183.01.T01:CDS
AATGGAAGTCGAAGAGCTTA+AGG 0.422589 1:-34390530 MsG0180002183.01.T01:CDS
CCACATCGCATGTTATCAAA+AGG 0.430095 1:+34390402 None:intergenic
CAGAAGAAGTTGAAGTAGTC+AGG 0.435981 1:+34390327 None:intergenic
TAGGACCGTTGCGTTTGGAT+TGG 0.454200 1:-34390724 MsG0180002183.01.T01:CDS
GGACTATAGTGTCGAGTTAG+TGG 0.460930 1:-34390703 MsG0180002183.01.T01:CDS
CAACTTCTTCTGGCCACCTC+CGG 0.471703 1:-34390316 MsG0180002183.01.T01:CDS
TAAGCTTAATGAGAAGAAGA+AGG 0.474353 1:-34390553 MsG0180002183.01.T01:CDS
AGAAAATACGTTGACATGCA+TGG 0.496549 1:+34390795 None:intergenic
TTAATGAGAAGAAGAAGGAA+TGG 0.498438 1:-34390548 MsG0180002183.01.T01:CDS
ATGGAAGTCGAAGAGCTTAA+GGG 0.506592 1:-34390529 MsG0180002183.01.T01:CDS
TTCACTGCAGCGAAGAGCGT+TGG 0.509094 1:-34390827 MsG0180002183.01.T01:CDS
TCGAGAATCCACTCCGGAGG+TGG 0.511300 1:+34390303 None:intergenic
AAAGACGACACAATTCCCTT+TGG 0.513520 1:+34390443 None:intergenic
TTTGGGTTCCCTAGCAAAGA+GGG 0.514334 1:-34390594 MsG0180002183.01.T01:CDS
CAGATTTGTCTATTGCCAAA+GGG 0.522486 1:-34390458 MsG0180002183.01.T01:CDS
GCTAGGGAACCCAAAACAAT+GGG 0.530183 1:+34390602 None:intergenic
AGTCAGGATAATCAGACAAA+GGG 0.533962 1:+34390343 None:intergenic
TCAGATTTGTCTATTGCCAA+AGG 0.535639 1:-34390459 MsG0180002183.01.T01:CDS
GAAAATACGTTGACATGCAT+GGG 0.536010 1:+34390796 None:intergenic
CTCGGGACATTGAACCAAAA+CGG 0.542934 1:-34390864 MsG0180002183.01.T01:CDS
ATGCAGTTAATAAAATTGGT+AGG 0.543247 1:-34390743 MsG0180002183.01.T01:CDS
ACTCACCTTCACACTCTACA+AGG 0.545195 1:+34390649 None:intergenic
CACATCGCATGTTATCAAAA+GGG 0.547013 1:+34390403 None:intergenic
AATCAGACAAAGGGATAAGC+CGG 0.556570 1:+34390352 None:intergenic
TGCTAGGGAACCCAAAACAA+TGG 0.570316 1:+34390601 None:intergenic
CAAAGAGGGTCTAAAACTGA+AGG 0.579762 1:-34390580 MsG0180002183.01.T01:CDS
TCTTCTGGCCACCTCCGGAG+TGG 0.587712 1:-34390311 MsG0180002183.01.T01:CDS
TAGTCAGGATAATCAGACAA+AGG 0.606271 1:+34390342 None:intergenic
TTAGTGGCTGAACATGTGAA+TGG 0.607438 1:-34390687 MsG0180002183.01.T01:CDS
TCGCTGCAGTGAAACAACAC+TGG 0.608391 1:+34390837 None:intergenic
ATATCTGCGCGTTTAAAGAG+CGG 0.622196 1:-34390770 MsG0180002183.01.T01:CDS
TGGCTGAACATGTGAATGGA+GGG 0.622410 1:-34390683 MsG0180002183.01.T01:CDS
TGAAGGTGAGTTGTTGCTAG+TGG 0.626191 1:-34390637 MsG0180002183.01.T01:CDS
GTGGCTGAACATGTGAATGG+AGG 0.661757 1:-34390684 MsG0180002183.01.T01:CDS
ATTTCGAGAATCCACTCCGG+AGG 0.662099 1:+34390300 None:intergenic
AAATTCCTTGTAGAGTGTGA+AGG 0.674175 1:-34390654 MsG0180002183.01.T01:CDS
TGCATTTCGAGAATCCACTC+CGG 0.678133 1:+34390297 None:intergenic
ATAGTCCAATCCAAACGCAA+CGG 0.688094 1:+34390719 None:intergenic
GGCTGAACATGTGAATGGAG+GGG 0.690996 1:-34390682 MsG0180002183.01.T01:CDS
TAGTTGCTATTATGCAACGT+GGG 0.698080 1:-34390908 MsG0180002183.01.T01:CDS
GTAGTTGCTATTATGCAACG+TGG 0.761838 1:-34390909 MsG0180002183.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATGCAGTTAATAAAAT+TGG - Chr1:34390458-34390477 MsG0180002183.01.T01:CDS 15.0%
! ATGCAGTTAATAAAATTGGT+AGG - Chr1:34390462-34390481 MsG0180002183.01.T01:CDS 25.0%
AACAAAAAAACTGTTGCTCT+CGG - Chr1:34390323-34390342 MsG0180002183.01.T01:CDS 30.0%
TAAGCTTAATGAGAAGAAGA+AGG - Chr1:34390652-34390671 MsG0180002183.01.T01:CDS 30.0%
TTAATGAGAAGAAGAAGGAA+TGG - Chr1:34390657-34390676 MsG0180002183.01.T01:CDS 30.0%
AAATTCCTTGTAGAGTGTGA+AGG - Chr1:34390551-34390570 MsG0180002183.01.T01:CDS 35.0%
ACAAAAAAACTGTTGCTCTC+GGG - Chr1:34390324-34390343 MsG0180002183.01.T01:CDS 35.0%
AGAAAATACGTTGACATGCA+TGG + Chr1:34390413-34390432 None:intergenic 35.0%
AGTCAGGATAATCAGACAAA+GGG + Chr1:34390865-34390884 None:intergenic 35.0%
CACATCGCATGTTATCAAAA+GGG + Chr1:34390805-34390824 None:intergenic 35.0%
CAGATTTGTCTATTGCCAAA+GGG - Chr1:34390747-34390766 MsG0180002183.01.T01:CDS 35.0%
GAAAATACGTTGACATGCAT+GGG + Chr1:34390412-34390431 None:intergenic 35.0%
TAGTCAGGATAATCAGACAA+AGG + Chr1:34390866-34390885 None:intergenic 35.0%
TCAGATTTGTCTATTGCCAA+AGG - Chr1:34390746-34390765 MsG0180002183.01.T01:CDS 35.0%
! ATCTATGACTCCCATTGTTT+TGG - Chr1:34390593-34390612 MsG0180002183.01.T01:CDS 35.0%
! TAGTTGCTATTATGCAACGT+GGG - Chr1:34390297-34390316 MsG0180002183.01.T01:CDS 35.0%
! TCTATGACTCCCATTGTTTT+GGG - Chr1:34390594-34390613 MsG0180002183.01.T01:CDS 35.0%
! TGGGATGTGTGTTTTTCATT+TGG - Chr1:34390823-34390842 MsG0180002183.01.T01:CDS 35.0%
! TTTTTCATTTGGACGAACAC+CGG - Chr1:34390834-34390853 MsG0180002183.01.T01:CDS 35.0%
!! CTTTTGATAACATGCGATGT+GGG - Chr1:34390804-34390823 MsG0180002183.01.T01:CDS 35.0%
!!! AAGGGATAAGGTTTTGTTCT+TGG - Chr1:34390694-34390713 MsG0180002183.01.T01:CDS 35.0%
!!! AAGGTTTTGTTCTTGGGAAA+TGG - Chr1:34390701-34390720 MsG0180002183.01.T01:CDS 35.0%
!!! AGGGATAAGGTTTTGTTCTT+GGG - Chr1:34390695-34390714 MsG0180002183.01.T01:CDS 35.0%
AAAGACGACACAATTCCCTT+TGG + Chr1:34390765-34390784 None:intergenic 40.0%
AATCAGACAAAGGGATAAGC+CGG + Chr1:34390856-34390875 None:intergenic 40.0%
AATGGAAGTCGAAGAGCTTA+AGG - Chr1:34390675-34390694 MsG0180002183.01.T01:CDS 40.0%
ATAGTCCAATCCAAACGCAA+CGG + Chr1:34390489-34390508 None:intergenic 40.0%
ATATCTGCGCGTTTAAAGAG+CGG - Chr1:34390435-34390454 MsG0180002183.01.T01:CDS 40.0%
ATGGAAGTCGAAGAGCTTAA+GGG - Chr1:34390676-34390695 MsG0180002183.01.T01:CDS 40.0%
CAGAAGAAGTTGAAGTAGTC+AGG + Chr1:34390881-34390900 None:intergenic 40.0%
CCACATCGCATGTTATCAAA+AGG + Chr1:34390806-34390825 None:intergenic 40.0%
CTGACTACTTCAACTTCTTC+TGG - Chr1:34390879-34390898 MsG0180002183.01.T01:CDS 40.0%
TTAGTGGCTGAACATGTGAA+TGG - Chr1:34390518-34390537 MsG0180002183.01.T01:CDS 40.0%
! CAAAGAGGGTCTAAAACTGA+AGG - Chr1:34390625-34390644 MsG0180002183.01.T01:CDS 40.0%
! GTAGTTGCTATTATGCAACG+TGG - Chr1:34390296-34390315 MsG0180002183.01.T01:CDS 40.0%
!! CCTTTTGATAACATGCGATG+TGG - Chr1:34390803-34390822 MsG0180002183.01.T01:CDS 40.0%
!! GTTTTAGACCCTCTTTGCTA+GGG + Chr1:34390622-34390641 None:intergenic 40.0%
!!! AGTTTTAGACCCTCTTTGCT+AGG + Chr1:34390623-34390642 None:intergenic 40.0%
ACTCACCTTCACACTCTACA+AGG + Chr1:34390559-34390578 None:intergenic 45.0%
CTCGGGACATTGAACCAAAA+CGG - Chr1:34390341-34390360 MsG0180002183.01.T01:CDS 45.0%
GCTAGGGAACCCAAAACAAT+GGG + Chr1:34390606-34390625 None:intergenic 45.0%
GTCGAAGAGCTTAAGGGATA+AGG - Chr1:34390682-34390701 MsG0180002183.01.T01:CDS 45.0%
TGAAGGTGAGTTGTTGCTAG+TGG - Chr1:34390568-34390587 MsG0180002183.01.T01:CDS 45.0%
TGCATTTCGAGAATCCACTC+CGG + Chr1:34390911-34390930 None:intergenic 45.0%
TGCTAGGGAACCCAAAACAA+TGG + Chr1:34390607-34390626 None:intergenic 45.0%
TGGCTGAACATGTGAATGGA+GGG - Chr1:34390522-34390541 MsG0180002183.01.T01:CDS 45.0%
! ACAACACTGGATGACCGTTT+TGG + Chr1:34390358-34390377 None:intergenic 45.0%
! GGACTATAGTGTCGAGTTAG+TGG - Chr1:34390502-34390521 MsG0180002183.01.T01:CDS 45.0%
! TTTGGGTTCCCTAGCAAAGA+GGG - Chr1:34390611-34390630 MsG0180002183.01.T01:CDS 45.0%
!!! TTTTGGGTTCCCTAGCAAAG+AGG - Chr1:34390610-34390629 MsG0180002183.01.T01:CDS 45.0%
ATTGGTAGGACCGTTGCGTT+TGG - Chr1:34390476-34390495 MsG0180002183.01.T01:CDS 50.0%
ATTTCGAGAATCCACTCCGG+AGG + Chr1:34390908-34390927 None:intergenic 50.0%
GGCTGAACATGTGAATGGAG+GGG - Chr1:34390523-34390542 MsG0180002183.01.T01:CDS 50.0%
GTGGCTGAACATGTGAATGG+AGG - Chr1:34390521-34390540 MsG0180002183.01.T01:CDS 50.0%
TAGGACCGTTGCGTTTGGAT+TGG - Chr1:34390481-34390500 MsG0180002183.01.T01:CDS 50.0%
TCGCTGCAGTGAAACAACAC+TGG + Chr1:34390371-34390390 None:intergenic 50.0%
CAACTTCTTCTGGCCACCTC+CGG - Chr1:34390889-34390908 MsG0180002183.01.T01:CDS 55.0%
! TTCACTGCAGCGAAGAGCGT+TGG - Chr1:34390378-34390397 MsG0180002183.01.T01:CDS 55.0%
TCGAGAATCCACTCCGGAGG+TGG + Chr1:34390905-34390924 None:intergenic 60.0%
TCTTCTGGCCACCTCCGGAG+TGG - Chr1:34390894-34390913 MsG0180002183.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 34390284 34390943 34390284 ID=MsG0180002183.01;Name=MsG0180002183.01
Chr1 mRNA 34390284 34390943 34390284 ID=MsG0180002183.01.T01;Parent=MsG0180002183.01;Name=MsG0180002183.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|219
Chr1 exon 34390284 34390943 34390284 ID=MsG0180002183.01.T01:exon:36184;Parent=MsG0180002183.01.T01
Chr1 CDS 34390284 34390943 34390284 ID=MsG0180002183.01.T01:cds;Parent=MsG0180002183.01.T01
Gene Sequence

>MsG0180002183.01.T01

ATGGGAAAAAAGGTAGTTGCTATTATGCAACGTGGGAAAAACAAAAAAACTGTTGCTCTCGGGACATTGAACCAAAACGGTCATCCAGTGTTGTTTCACTGCAGCGAAGAGCGTTGGACGTTGATCCCATGCATGTCAACGTATTTTCTAGATATCTGCGCGTTTAAAGAGCGGTTTTATGCAGTTAATAAAATTGGTAGGACCGTTGCGTTTGGATTGGACTATAGTGTCGAGTTAGTGGCTGAACATGTGAATGGAGGGGACATGAAATTCCTTGTAGAGTGTGAAGGTGAGTTGTTGCTAGTGGACATCTATGACTCCCATTGTTTTGGGTTCCCTAGCAAAGAGGGTCTAAAACTGAAGGTGTTTAAGCTTAATGAGAAGAAGAAGGAATGGAAGTCGAAGAGCTTAAGGGATAAGGTTTTGTTCTTGGGAAATGGATGTTCGTTTACTGCTTCTGCTTCAGATTTGTCTATTGCCAAAGGGAATTGTGTCGTCTTTATTGACGATGCATTCATCCCTTTTGATAACATGCGATGTGGGATGTGTGTTTTTCATTTGGACGAACACCGGCTTATCCCTTTGTCTGATTATCCTGACTACTTCAACTTCTTCTGGCCACCTCCGGAGTGGATTCTCGAAATGCAATACCATTCATAA

Protein sequence

>MsG0180002183.01.T01

MGKKVVAIMQRGKNKKTVALGTLNQNGHPVLFHCSEERWTLIPCMSTYFLDICAFKERFYAVNKIGRTVAFGLDYSVELVAEHVNGGDMKFLVECEGELLLVDIYDSHCFGFPSKEGLKLKVFKLNEKKKEWKSKSLRDKVLFLGNGCSFTASASDLSIAKGNCVVFIDDAFIPFDNMRCGMCVFHLDEHRLIPLSDYPDYFNFFWPPPEWILEMQYHS*