Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002233.01.T01 | RHN65795.1 | 81.061 | 396 | 72 | 1 | 1 | 393 | 46 | 441 | 0 | 629 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002233.01.T01 | Q9S7T8 | 53.351 | 388 | 174 | 3 | 11 | 391 | 3 | 390 | 1.63E-141 | 411 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002233.01.T01 | A0A396IPH6 | 81.061 | 396 | 72 | 1 | 1 | 393 | 46 | 441 | 0.0 | 629 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002233.01 | MsG0180002234.01 | 0.915505 | 5.436200e-85 | 1.425362e-80 |
| MsG0180002233.01 | MsG0180002327.01 | 0.815424 | 9.313213e-52 | 6.418470e-49 |
| MsG0180002233.01 | MsG0280006325.01 | 0.814678 | 1.363041e-51 | 9.205316e-49 |
| MsG0180002233.01 | MsG0280006551.01 | 0.803743 | 3.013844e-49 | 1.526449e-46 |
| MsG0180002233.01 | MsG0280008759.01 | 0.813511 | 2.467287e-51 | 1.614453e-48 |
| MsG0180002233.01 | MsG0280009248.01 | 0.822233 | 2.643606e-53 | 2.199765e-50 |
| MsG0180002233.01 | MsG0280010117.01 | 0.803913 | 2.777601e-49 | 1.413068e-46 |
| MsG0180002233.01 | MsG0380011910.01 | 0.808059 | 3.730863e-50 | 2.111963e-47 |
| MsG0180002233.01 | MsG0380012215.01 | 0.877029 | 8.716478e-69 | 4.317923e-65 |
| MsG0180002233.01 | MsG0380013883.01 | 0.800016 | 1.755855e-48 | 8.088638e-46 |
| MsG0180002233.01 | MsG0380014977.01 | 0.802748 | 4.841134e-49 | 2.390308e-46 |
| MsG0180002233.01 | MsG0380017820.01 | 0.813857 | 2.069751e-51 | 1.367053e-48 |
| MsG0180002233.01 | MsG0380017822.01 | 0.817860 | 2.648414e-52 | 1.951199e-49 |
| MsG0180002233.01 | MsG0380017855.01 | 0.811781 | 5.896212e-51 | 3.682966e-48 |
| MsG0180002233.01 | MsG0480018664.01 | 0.816797 | 4.594243e-52 | 3.287479e-49 |
| MsG0180002233.01 | MsG0480021587.01 | 0.801120 | 1.045965e-48 | 4.955368e-46 |
| MsG0180002233.01 | MsG0580026222.01 | 0.834855 | 2.354414e-56 | 2.829047e-53 |
| MsG0180002233.01 | MsG0580026303.01 | 0.817697 | 2.882459e-52 | 2.114117e-49 |
| MsG0180002233.01 | MsG0580026434.01 | 0.800689 | 1.281122e-48 | 6.003255e-46 |
| MsG0180002233.01 | MsG0580028169.01 | 0.806570 | 7.714729e-50 | 4.201469e-47 |
| MsG0180002233.01 | MsG0580028170.01 | 0.806736 | 7.115726e-50 | 3.891864e-47 |
| MsG0180002233.01 | MsG0580029381.01 | 0.825524 | 4.472439e-54 | 4.086440e-51 |
| MsG0180002233.01 | MsG0580030050.01 | 0.822325 | 2.516725e-53 | 2.099754e-50 |
| MsG0180002233.01 | MsG0680032158.01 | 0.823798 | 1.141036e-53 | 9.925819e-51 |
| MsG0180002233.01 | MsG0680035115.01 | 0.805286 | 1.436170e-49 | 7.568471e-47 |
| MsG0180002233.01 | MsG0780036080.01 | 0.803207 | 3.892559e-49 | 1.944485e-46 |
| MsG0180002233.01 | MsG0780037606.01 | 0.818121 | 2.311866e-52 | 1.715469e-49 |
| MsG0180002233.01 | MsG0780039282.01 | 0.824781 | 6.701965e-54 | 5.995683e-51 |
| MsG0180002233.01 | MsG0880042454.01 | 0.830314 | 3.149782e-55 | 3.306496e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002233.01.T01 | MTR_3g018740 | 82.383 | 386 | 65 | 1 | 11 | 393 | 3 | 388 | 0.0 | 619 |
| MsG0180002233.01.T01 | MTR_3g101010 | 72.280 | 386 | 101 | 2 | 11 | 391 | 3 | 387 | 0.0 | 522 |
| MsG0180002233.01.T01 | MTR_8g027395 | 67.008 | 391 | 125 | 3 | 3 | 391 | 7 | 395 | 0.0 | 508 |
| MsG0180002233.01.T01 | MTR_2g045980 | 67.436 | 390 | 116 | 5 | 11 | 393 | 3 | 388 | 1.27e-178 | 502 |
| MsG0180002233.01.T01 | MTR_4g045707 | 66.580 | 383 | 124 | 2 | 12 | 391 | 5 | 386 | 3.89e-177 | 498 |
| MsG0180002233.01.T01 | MTR_2g046030 | 66.154 | 390 | 121 | 4 | 11 | 393 | 3 | 388 | 4.74e-177 | 498 |
| MsG0180002233.01.T01 | MTR_8g027420 | 67.102 | 383 | 111 | 4 | 11 | 391 | 4 | 373 | 3.28e-173 | 488 |
| MsG0180002233.01.T01 | MTR_3g101180 | 64.304 | 395 | 123 | 6 | 13 | 391 | 6 | 398 | 1.82e-170 | 482 |
| MsG0180002233.01.T01 | MTR_8g461160 | 65.641 | 390 | 124 | 4 | 13 | 393 | 6 | 394 | 2.09e-170 | 482 |
| MsG0180002233.01.T01 | MTR_3g048000 | 63.255 | 381 | 119 | 4 | 14 | 391 | 28 | 390 | 5.70e-166 | 470 |
| MsG0180002233.01.T01 | MTR_3g101030 | 63.224 | 397 | 120 | 5 | 18 | 391 | 34 | 427 | 8.92e-166 | 471 |
| MsG0180002233.01.T01 | MTR_7g451890 | 59.398 | 399 | 152 | 3 | 3 | 392 | 4 | 401 | 4.59e-165 | 468 |
| MsG0180002233.01.T01 | MTR_7g050810 | 60.250 | 400 | 146 | 4 | 5 | 392 | 5 | 403 | 9.49e-165 | 468 |
| MsG0180002233.01.T01 | MTR_7g047780 | 60.150 | 399 | 149 | 4 | 3 | 392 | 4 | 401 | 2.42e-163 | 464 |
| MsG0180002233.01.T01 | MTR_4g088535 | 66.138 | 378 | 108 | 5 | 13 | 388 | 7 | 366 | 1.27e-162 | 461 |
| MsG0180002233.01.T01 | MTR_4g088525 | 64.722 | 360 | 125 | 2 | 33 | 391 | 9 | 367 | 2.64e-160 | 455 |
| MsG0180002233.01.T01 | MTR_3g101130 | 62.371 | 388 | 133 | 5 | 12 | 391 | 5 | 387 | 6.88e-160 | 455 |
| MsG0180002233.01.T01 | MTR_7g050750 | 59.439 | 392 | 149 | 4 | 10 | 392 | 5 | 395 | 8.57e-159 | 452 |
| MsG0180002233.01.T01 | MTR_3g101050 | 63.708 | 383 | 114 | 7 | 16 | 391 | 32 | 396 | 5.54e-151 | 432 |
| MsG0180002233.01.T01 | MTR_3g015620 | 60.969 | 392 | 119 | 4 | 10 | 392 | 13 | 379 | 1.39e-150 | 431 |
| MsG0180002233.01.T01 | MTR_3g018747 | 73.846 | 260 | 50 | 2 | 138 | 393 | 10 | 255 | 3.68e-128 | 369 |
| MsG0180002233.01.T01 | MTR_3g102920 | 53.415 | 410 | 133 | 8 | 4 | 391 | 35 | 408 | 7.15e-128 | 374 |
| MsG0180002233.01.T01 | MTR_3g101190 | 55.091 | 383 | 95 | 6 | 11 | 391 | 3 | 310 | 1.97e-125 | 364 |
| MsG0180002233.01.T01 | MTR_3g101200 | 51.899 | 395 | 155 | 5 | 5 | 391 | 23 | 390 | 9.58e-119 | 350 |
| MsG0180002233.01.T01 | MTR_3g100995 | 64.122 | 262 | 87 | 2 | 135 | 392 | 5 | 263 | 2.01e-112 | 329 |
| MsG0180002233.01.T01 | MTR_7g050830 | 52.778 | 324 | 120 | 3 | 3 | 304 | 6 | 318 | 4.20e-111 | 328 |
| MsG0180002233.01.T01 | MTR_3g100010 | 55.769 | 312 | 90 | 3 | 36 | 314 | 40 | 336 | 2.41e-105 | 314 |
| MsG0180002233.01.T01 | MTR_3g015760 | 59.928 | 277 | 101 | 3 | 122 | 392 | 8 | 280 | 1.54e-100 | 300 |
| MsG0180002233.01.T01 | MTR_3g111160 | 65.596 | 218 | 71 | 2 | 178 | 391 | 1 | 218 | 6.33e-90 | 270 |
| MsG0180002233.01.T01 | MTR_3g114310 | 42.347 | 392 | 207 | 11 | 14 | 390 | 20 | 407 | 5.44e-84 | 262 |
| MsG0180002233.01.T01 | MTR_3g100520 | 62.037 | 216 | 71 | 3 | 178 | 391 | 1 | 207 | 6.57e-83 | 252 |
| MsG0180002233.01.T01 | MTR_2g046410 | 64.251 | 207 | 66 | 3 | 194 | 393 | 144 | 349 | 1.64e-81 | 254 |
| MsG0180002233.01.T01 | MTR_3g101110 | 57.806 | 237 | 66 | 3 | 139 | 367 | 70 | 280 | 6.26e-81 | 250 |
| MsG0180002233.01.T01 | MTR_3g099970 | 60.204 | 196 | 60 | 2 | 138 | 333 | 21 | 198 | 4.13e-73 | 227 |
| MsG0180002233.01.T01 | MTR_3g111150 | 63.636 | 176 | 40 | 1 | 15 | 190 | 1 | 152 | 6.95e-69 | 215 |
| MsG0180002233.01.T01 | MTR_3g101150 | 63.924 | 158 | 47 | 2 | 243 | 391 | 1 | 157 | 1.07e-60 | 193 |
| MsG0180002233.01.T01 | MTR_3g102870 | 59.146 | 164 | 57 | 2 | 200 | 354 | 1 | 163 | 5.38e-56 | 182 |
| MsG0180002233.01.T01 | MTR_3g101020 | 53.763 | 186 | 55 | 2 | 211 | 392 | 1 | 159 | 1.32e-53 | 175 |
| MsG0180002233.01.T01 | MTR_3g100990 | 73.984 | 123 | 32 | 0 | 12 | 134 | 4 | 126 | 7.12e-52 | 181 |
| MsG0180002233.01.T01 | MTR_4g093550 | 67.045 | 88 | 29 | 0 | 9 | 96 | 2 | 89 | 2.73e-29 | 109 |
| MsG0180002233.01.T01 | MTR_6g046890 | 65.753 | 73 | 20 | 1 | 32 | 99 | 18 | 90 | 4.20e-21 | 89.0 |
| MsG0180002233.01.T01 | MTR_3g101140 | 59.459 | 74 | 23 | 2 | 324 | 391 | 42 | 114 | 2.68e-17 | 77.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002233.01.T01 | AT1G47710 | 53.351 | 388 | 174 | 3 | 11 | 391 | 3 | 390 | 1.67e-142 | 411 |
| MsG0180002233.01.T01 | AT1G47710 | 53.351 | 388 | 174 | 3 | 11 | 391 | 30 | 417 | 7.49e-142 | 410 |
| MsG0180002233.01.T01 | AT3G45220 | 48.462 | 390 | 189 | 5 | 11 | 390 | 3 | 390 | 2.02e-123 | 362 |
| MsG0180002233.01.T01 | AT2G25240 | 49.105 | 391 | 177 | 7 | 11 | 390 | 3 | 382 | 6.60e-117 | 345 |
| MsG0180002233.01.T01 | AT2G26390 | 48.731 | 394 | 178 | 7 | 11 | 390 | 3 | 386 | 1.68e-116 | 345 |
| MsG0180002233.01.T01 | AT1G64030 | 41.432 | 391 | 196 | 6 | 11 | 390 | 3 | 371 | 5.52e-97 | 295 |
| MsG0180002233.01.T01 | AT2G14540 | 41.814 | 397 | 198 | 7 | 4 | 390 | 24 | 397 | 2.22e-94 | 288 |
| MsG0180002233.01.T01 | AT1G62170 | 41.282 | 390 | 193 | 7 | 13 | 390 | 66 | 431 | 7.24e-92 | 283 |
| MsG0180002233.01.T01 | AT2G35580 | 42.967 | 391 | 176 | 9 | 11 | 390 | 17 | 371 | 1.05e-91 | 281 |
| MsG0180002233.01.T01 | AT1G62170 | 38.298 | 423 | 192 | 8 | 13 | 390 | 66 | 464 | 1.18e-85 | 268 |
| MsG0180002233.01.T01 | AT1G64010 | 44.240 | 217 | 95 | 4 | 178 | 390 | 1 | 195 | 8.06e-53 | 174 |
| MsG0180002233.01.T01 | AT1G64010 | 43.564 | 202 | 88 | 4 | 193 | 390 | 2 | 181 | 8.60e-46 | 156 |
| MsG0180002233.01.T01 | AT1G62160 | 41.243 | 177 | 81 | 7 | 217 | 390 | 62 | 218 | 2.42e-30 | 116 |
| MsG0180002233.01.T01 | AT1G63280 | 49.153 | 118 | 56 | 2 | 134 | 249 | 5 | 120 | 3.65e-29 | 110 |
| MsG0180002233.01.T01 | AT1G51330 | 48.673 | 113 | 53 | 2 | 135 | 243 | 29 | 140 | 1.37e-24 | 100 |
| MsG0180002233.01.T01 | AT1G64020 | 33.036 | 112 | 72 | 3 | 18 | 127 | 10 | 120 | 5.22e-13 | 65.9 |
Find 77 sgRNAs with CRISPR-Local
Find 118 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTAATGCACTCTACTTTAA+AGG | 0.141946 | 1:+35191550 | MsG0180002233.01.T01:CDS |
| TGACTAAAGAAGTGAATTTA+TGG | 0.193244 | 1:+35191449 | MsG0180002233.01.T01:CDS |
| GAGGAAGAAGTTCTTTAATA+AGG | 0.287252 | 1:-35191492 | None:intergenic |
| GTTGAGATCATCGGTGGATT+TGG | 0.331266 | 1:-35190641 | None:intergenic |
| CAATGCTAACAGTCCATCTT+TGG | 0.352985 | 1:-35191776 | None:intergenic |
| ACTGGAACCATTCTCTTTGT+TGG | 0.359618 | 1:+35192159 | MsG0180002233.01.T01:CDS |
| TATTTCTGATTAGAGAAGAT+TGG | 0.360147 | 1:+35192136 | MsG0180002233.01.T01:CDS |
| CTCTCAGACGCCGCTTTCAC+TGG | 0.370831 | 1:+35190694 | MsG0180002233.01.T01:CDS |
| CTTCGTCTTCTTTATAAGAA+AGG | 0.383719 | 1:+35191709 | MsG0180002233.01.T01:CDS |
| GTTAAGCTATCAATTGATCT+TGG | 0.385492 | 1:-35191514 | None:intergenic |
| TCTCATATGTTGAAGGAGTT+AGG | 0.397032 | 1:+35191913 | MsG0180002233.01.T01:CDS |
| AACAGTCCATCTTTGGCATC+TGG | 0.409162 | 1:-35191769 | None:intergenic |
| TGAAACTTCTCATATGTTGA+AGG | 0.410996 | 1:+35191906 | MsG0180002233.01.T01:CDS |
| TGCCGAGGGAAACATTTGTT+TGG | 0.416643 | 1:-35190483 | None:intergenic |
| GGAAGACAAGATTCCTTTGA+AGG | 0.427458 | 1:-35190779 | None:intergenic |
| TTCTGTCGAGAACAGATGTT+TGG | 0.430392 | 1:-35190509 | None:intergenic |
| GGACAAAGTAGATTTATAAC+TGG | 0.434487 | 1:-35190800 | None:intergenic |
| TCTCTTCAACCTTCCTTCAA+AGG | 0.444853 | 1:+35190766 | MsG0180002233.01.T01:CDS |
| GAAGGAAGGTTGAAGAGAAA+GGG | 0.450222 | 1:-35190761 | None:intergenic |
| TTTCTTCCAGATGCCAAAGA+TGG | 0.454297 | 1:+35191763 | MsG0180002233.01.T01:CDS |
| GACTAAAGAAGTGAATTTAT+GGG | 0.454605 | 1:+35191450 | MsG0180002233.01.T01:CDS |
| TGAAGGAAGGTTGAAGAGAA+AGG | 0.456356 | 1:-35190762 | None:intergenic |
| TTCATTGAAGTAAATGAAGA+AGG | 0.457204 | 1:+35192033 | MsG0180002233.01.T01:CDS |
| GTTAGCATTGGTGGAGAAGT+TGG | 0.459448 | 1:+35191789 | MsG0180002233.01.T01:CDS |
| AGATGGACTGTTAGCATTGG+TGG | 0.461266 | 1:+35191780 | MsG0180002233.01.T01:CDS |
| CGATGATGCTTAGCACAACT+TGG | 0.463699 | 1:-35190567 | None:intergenic |
| GAAGCTGCTGCAGCTAGTGC+TGG | 0.463886 | 1:+35192060 | MsG0180002233.01.T01:CDS |
| TGACTGAGCTTCCATTCAAA+AGG | 0.469619 | 1:-35191624 | None:intergenic |
| CAGACGCCGCTTTCACTGGC+GGG | 0.477109 | 1:+35190698 | MsG0180002233.01.T01:CDS |
| TCTCTAATCAGAAATAAGAA+AGG | 0.483189 | 1:-35192129 | None:intergenic |
| CATCCCCTCGGTGGTCATGA+AGG | 0.485289 | 1:-35191653 | None:intergenic |
| AGACTATCGTGTAGTAAGTC+AGG | 0.494602 | 1:-35191820 | None:intergenic |
| GTGCTAAGCATCATCGCTGC+TGG | 0.494658 | 1:+35190574 | MsG0180002233.01.T01:CDS |
| TGCATTAGCAAAGATGAGTC+TGG | 0.499282 | 1:-35191536 | None:intergenic |
| CTGATTAGAGAAGATTGGAC+TGG | 0.501644 | 1:+35192141 | MsG0180002233.01.T01:CDS |
| GACAAGATTCCTTTGAAGGA+AGG | 0.508248 | 1:-35190775 | None:intergenic |
| TCATCTACTTCTACTTCAGC+AGG | 0.509971 | 1:-35191847 | None:intergenic |
| ATCATCGCTGCTGGCTCTGA+GGG | 0.511801 | 1:+35190583 | MsG0180002233.01.T01:CDS |
| CACTCTACTTTAAAGGAGCA+TGG | 0.514338 | 1:+35191557 | MsG0180002233.01.T01:CDS |
| CTCTAATCAGAAATAAGAAA+GGG | 0.516757 | 1:-35192128 | None:intergenic |
| CATCATCGCTGCTGGCTCTG+AGG | 0.516897 | 1:+35190582 | MsG0180002233.01.T01:CDS |
| GTCAAACCTCCAGGAGTGAA+AGG | 0.524050 | 1:-35191946 | None:intergenic |
| TTTGGTTGGTGATTGATTCA+CGG | 0.528455 | 1:-35190465 | None:intergenic |
| CACTTGTCCAACAAAGAGAA+TGG | 0.528533 | 1:-35192166 | None:intergenic |
| AAGCTATCAATTGATCTTGG+AGG | 0.537062 | 1:-35191511 | None:intergenic |
| ATCAATAAACTGCATCCCCT+CGG | 0.537857 | 1:-35191665 | None:intergenic |
| TGCTAAGATCACTGATCAAG+AGG | 0.544299 | 1:-35192195 | None:intergenic |
| TCAGACGCCGCTTTCACTGG+CGG | 0.550246 | 1:+35190697 | MsG0180002233.01.T01:CDS |
| GCGAGGCCCGCCAGTGAAAG+CGG | 0.556128 | 1:-35190704 | None:intergenic |
| GAAATATATTAGAAACAGAA+AGG | 0.558448 | 1:-35192002 | None:intergenic |
| GAGGGAAACATTTGTTTGGT+TGG | 0.569951 | 1:-35190479 | None:intergenic |
| ATCCCCTCGGTGGTCATGAA+GGG | 0.571981 | 1:-35191652 | None:intergenic |
| AACCAAACAAATGTTTCCCT+CGG | 0.575261 | 1:+35190481 | MsG0180002233.01.T01:CDS |
| GGCGAGGTTGTTGAGATCAT+CGG | 0.582316 | 1:-35190650 | None:intergenic |
| GTCGAGAAGCTGTTGTTGTG+TGG | 0.586119 | 1:-35190608 | None:intergenic |
| TTTGAATGGAAGCTCAGTCA+AGG | 0.589562 | 1:+35191627 | MsG0180002233.01.T01:CDS |
| AACAAATGTTTCCCTCGGCA+TGG | 0.592617 | 1:+35190486 | MsG0180002233.01.T01:CDS |
| ACAGATGTTTGGCCATGCCG+AGG | 0.599139 | 1:-35190498 | None:intergenic |
| CAGATGTTTGGCCATGCCGA+GGG | 0.601831 | 1:-35190497 | None:intergenic |
| GTTCCCTTCATGACCACCGA+GGG | 0.602185 | 1:+35191649 | MsG0180002233.01.T01:CDS |
| TCGAGAAGCTGTTGTTGTGT+GGG | 0.605173 | 1:-35190607 | None:intergenic |
| GACGATAGAAACAAGGTGAG+AGG | 0.606218 | 1:-35190671 | None:intergenic |
| TATGTTGAAGGAGTTAGGAG+TGG | 0.615125 | 1:+35191918 | MsG0180002233.01.T01:CDS |
| CTGAGAGGACGATAGAAACA+AGG | 0.615824 | 1:-35190678 | None:intergenic |
| CATCGGTGGATTTGGACTCG+AGG | 0.615894 | 1:-35190633 | None:intergenic |
| CAGTGAAAGCGGCGTCTGAG+AGG | 0.622496 | 1:-35190693 | None:intergenic |
| AGCACAACTTGGAGTGACAA+TGG | 0.624764 | 1:-35190556 | None:intergenic |
| TAATCAGAAATAAGAAAGGG+TGG | 0.640706 | 1:-35192125 | None:intergenic |
| CAAAGATGGACTGTTAGCAT+TGG | 0.645848 | 1:+35191777 | MsG0180002233.01.T01:CDS |
| GAGGTTGTTGAGATCATCGG+TGG | 0.666044 | 1:-35190647 | None:intergenic |
| AATAAACTGCATCCCCTCGG+TGG | 0.668287 | 1:-35191662 | None:intergenic |
| TAGAAACAAGGTGAGAGGCG+AGG | 0.684792 | 1:-35190666 | None:intergenic |
| ATTGGTTATGAAATCAACAG+AGG | 0.690132 | 1:-35190821 | None:intergenic |
| ATACACCATCAACAAAAGAG+AGG | 0.697514 | 1:-35190726 | None:intergenic |
| CCATCAACAAAAGAGAGGCG+AGG | 0.704628 | 1:-35190721 | None:intergenic |
| TTCCCTTCATGACCACCGAG+GGG | 0.716157 | 1:+35191650 | MsG0180002233.01.T01:CDS |
| GGTTCCCTTCATGACCACCG+AGG | 0.739875 | 1:+35191648 | MsG0180002233.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTCATGTTAATTAATTAA+AGG | + | Chr1:35190895-35190914 | MsG0180002233.01.T01:intron | 10.0% |
| !! | TTTCATGTTAATTAATTAAA+GGG | + | Chr1:35190896-35190915 | MsG0180002233.01.T01:intron | 10.0% |
| !!! | TAAATTTGATATTGTTTTGA+AGG | + | Chr1:35191417-35191436 | MsG0180002233.01.T01:intron | 15.0% |
| !!! | TAATTTTTTTTGTTTTGAAG+AGG | - | Chr1:35191174-35191193 | None:intergenic | 15.0% |
| !! | GAAATATATTAGAAACAGAA+AGG | - | Chr1:35192005-35192024 | None:intergenic | 20.0% |
| !! | TAAACTAACCAGAAAATTTA+GGG | - | Chr1:35190950-35190969 | None:intergenic | 20.0% |
| !!! | TAAAGATACAGTATATGTTT+TGG | - | Chr1:35191016-35191035 | None:intergenic | 20.0% |
| !!! | TCATATGAAATTTTGAATCT+TGG | - | Chr1:35191883-35191902 | None:intergenic | 20.0% |
| ! | ATAGTAAAAGTTCATGACTT+TGG | + | Chr1:35191078-35191097 | MsG0180002233.01.T01:intron | 25.0% |
| ! | CAACTAATTTATCAACGTAA+CGG | + | Chr1:35191243-35191262 | MsG0180002233.01.T01:intron | 25.0% |
| ! | CTAAACTAACCAGAAAATTT+AGG | - | Chr1:35190951-35190970 | None:intergenic | 25.0% |
| ! | CTCTAATCAGAAATAAGAAA+GGG | - | Chr1:35192131-35192150 | None:intergenic | 25.0% |
| ! | GACTAAAGAAGTGAATTTAT+GGG | + | Chr1:35191450-35191469 | MsG0180002233.01.T01:CDS | 25.0% |
| ! | TCTCTAATCAGAAATAAGAA+AGG | - | Chr1:35192132-35192151 | None:intergenic | 25.0% |
| ! | TGACTAAAGAAGTGAATTTA+TGG | + | Chr1:35191449-35191468 | MsG0180002233.01.T01:CDS | 25.0% |
| ! | TTCATTGAAGTAAATGAAGA+AGG | + | Chr1:35192033-35192052 | MsG0180002233.01.T01:CDS | 25.0% |
| ! | TTCTTTAATCAGTGTAGAAT+TGG | - | Chr1:35191110-35191129 | None:intergenic | 25.0% |
| !! | TATGATTTTCACCTTTTGAA+TGG | + | Chr1:35191613-35191632 | MsG0180002233.01.T01:CDS | 25.0% |
| !! | TATTTCTGATTAGAGAAGAT+TGG | + | Chr1:35192136-35192155 | MsG0180002233.01.T01:CDS | 25.0% |
| !!! | CAGTATATGTTTTGGTAAAT+TGG | - | Chr1:35191008-35191027 | None:intergenic | 25.0% |
| AGATAGATTGCTGTGAAAAA+AGG | - | Chr1:35190865-35190884 | None:intergenic | 30.0% | |
| ATTGGTTATGAAATCAACAG+AGG | - | Chr1:35190824-35190843 | None:intergenic | 30.0% | |
| CTTCGTCTTCTTTATAAGAA+AGG | + | Chr1:35191709-35191728 | MsG0180002233.01.T01:CDS | 30.0% | |
| GAGATATGTAACACAAACAT+TGG | - | Chr1:35190842-35190861 | None:intergenic | 30.0% | |
| GAGGAAGAAGTTCTTTAATA+AGG | - | Chr1:35191495-35191514 | None:intergenic | 30.0% | |
| GATAGATTGCTGTGAAAAAA+GGG | - | Chr1:35190864-35190883 | None:intergenic | 30.0% | |
| GGACAAAGTAGATTTATAAC+TGG | - | Chr1:35190803-35190822 | None:intergenic | 30.0% | |
| GGATGCTAGAAAACAATAAA+GGG | - | Chr1:35191224-35191243 | None:intergenic | 30.0% | |
| GTTAAGCTATCAATTGATCT+TGG | - | Chr1:35191517-35191536 | None:intergenic | 30.0% | |
| TAATCAGAAATAAGAAAGGG+TGG | - | Chr1:35192128-35192147 | None:intergenic | 30.0% | |
| TGAAACTTCTCATATGTTGA+AGG | + | Chr1:35191906-35191925 | MsG0180002233.01.T01:CDS | 30.0% | |
| TGGATGCTAGAAAACAATAA+AGG | - | Chr1:35191225-35191244 | None:intergenic | 30.0% | |
| ! | CAGAAAATTTAGGGTATACT+AGG | - | Chr1:35190941-35190960 | None:intergenic | 30.0% |
| ! | CGTTACGTTGATAAATTAGT+TGG | - | Chr1:35191245-35191264 | None:intergenic | 30.0% |
| ! | CTAGTATACCCTAAATTTTC+TGG | + | Chr1:35190939-35190958 | MsG0180002233.01.T01:intron | 30.0% |
| ! | CTTTATTTCAGCACAAAGTA+GGG | + | Chr1:35191031-35191050 | MsG0180002233.01.T01:intron | 30.0% |
| ! | TCTTTATTTCAGCACAAAGT+AGG | + | Chr1:35191030-35191049 | MsG0180002233.01.T01:intron | 30.0% |
| AACCAAACAAATGTTTCCCT+CGG | + | Chr1:35190481-35190500 | MsG0180002233.01.T01:CDS | 35.0% | |
| AAGCTATCAATTGATCTTGG+AGG | - | Chr1:35191514-35191533 | None:intergenic | 35.0% | |
| ATACACCATCAACAAAAGAG+AGG | - | Chr1:35190729-35190748 | None:intergenic | 35.0% | |
| GCTAATGCACTCTACTTTAA+AGG | + | Chr1:35191550-35191569 | MsG0180002233.01.T01:CDS | 35.0% | |
| TCTCATATGTTGAAGGAGTT+AGG | + | Chr1:35191913-35191932 | MsG0180002233.01.T01:CDS | 35.0% | |
| TTCAGTGTGATTACAAGTGT+TGG | - | Chr1:35191310-35191329 | None:intergenic | 35.0% | |
| ! | AAATTGGCATGTAGAAGCAT+TGG | - | Chr1:35190992-35191011 | None:intergenic | 35.0% |
| ! | TAACAAAGTGTTTCGTCACA+TGG | - | Chr1:35191147-35191166 | None:intergenic | 35.0% |
| ! | TTTTCACAAGCTATGAAGCA+CGG | - | Chr1:35191364-35191383 | None:intergenic | 35.0% |
| !! | TTTGGTTGGTGATTGATTCA+CGG | - | Chr1:35190468-35190487 | None:intergenic | 35.0% |
| !!! | CTCTTTTGTTGATGGTGTAT+GGG | + | Chr1:35190729-35190748 | MsG0180002233.01.T01:CDS | 35.0% |
| !!! | TCTCTTTTGTTGATGGTGTA+TGG | + | Chr1:35190728-35190747 | MsG0180002233.01.T01:CDS | 35.0% |
| ACTGGAACCATTCTCTTTGT+TGG | + | Chr1:35192159-35192178 | MsG0180002233.01.T01:CDS | 40.0% | |
| AGACTATCGTGTAGTAAGTC+AGG | - | Chr1:35191823-35191842 | None:intergenic | 40.0% | |
| ATCAATAAACTGCATCCCCT+CGG | - | Chr1:35191668-35191687 | None:intergenic | 40.0% | |
| CAATGCTAACAGTCCATCTT+TGG | - | Chr1:35191779-35191798 | None:intergenic | 40.0% | |
| CACTTGTCCAACAAAGAGAA+TGG | - | Chr1:35192169-35192188 | None:intergenic | 40.0% | |
| CTGATTAGAGAAGATTGGAC+TGG | + | Chr1:35192141-35192160 | MsG0180002233.01.T01:CDS | 40.0% | |
| GAAGGAAGGTTGAAGAGAAA+GGG | - | Chr1:35190764-35190783 | None:intergenic | 40.0% | |
| GAGGGAAACATTTGTTTGGT+TGG | - | Chr1:35190482-35190501 | None:intergenic | 40.0% | |
| GGAAGACAAGATTCCTTTGA+AGG | - | Chr1:35190782-35190801 | None:intergenic | 40.0% | |
| TATGTTGAAGGAGTTAGGAG+TGG | + | Chr1:35191918-35191937 | MsG0180002233.01.T01:CDS | 40.0% | |
| TCATCTACTTCTACTTCAGC+AGG | - | Chr1:35191850-35191869 | None:intergenic | 40.0% | |
| TCCTGGAGGTTTGACAAAAA+TGG | + | Chr1:35191954-35191973 | MsG0180002233.01.T01:CDS | 40.0% | |
| TCTCTTCAACCTTCCTTCAA+AGG | + | Chr1:35190766-35190785 | MsG0180002233.01.T01:CDS | 40.0% | |
| TGAAGGAAGGTTGAAGAGAA+AGG | - | Chr1:35190765-35190784 | None:intergenic | 40.0% | |
| TGACTGAGCTTCCATTCAAA+AGG | - | Chr1:35191627-35191646 | None:intergenic | 40.0% | |
| TGCATTAGCAAAGATGAGTC+TGG | - | Chr1:35191539-35191558 | None:intergenic | 40.0% | |
| TGGAGGTTTGACAAAAATGG+TGG | + | Chr1:35191957-35191976 | MsG0180002233.01.T01:CDS | 40.0% | |
| TTCTGTCGAGAACAGATGTT+TGG | - | Chr1:35190512-35190531 | None:intergenic | 40.0% | |
| TTTCTTCCAGATGCCAAAGA+TGG | + | Chr1:35191763-35191782 | MsG0180002233.01.T01:CDS | 40.0% | |
| TTTGAATGGAAGCTCAGTCA+AGG | + | Chr1:35191627-35191646 | MsG0180002233.01.T01:CDS | 40.0% | |
| ! | CACTCTACTTTAAAGGAGCA+TGG | + | Chr1:35191557-35191576 | MsG0180002233.01.T01:CDS | 40.0% |
| ! | GACAAGATTCCTTTGAAGGA+AGG | - | Chr1:35190778-35190797 | None:intergenic | 40.0% |
| !! | ACCATTTTTGTCAAACCTCC+AGG | - | Chr1:35191958-35191977 | None:intergenic | 40.0% |
| !! | CAAAGATGGACTGTTAGCAT+TGG | + | Chr1:35191777-35191796 | MsG0180002233.01.T01:CDS | 40.0% |
| AACAAATGTTTCCCTCGGCA+TGG | + | Chr1:35190486-35190505 | MsG0180002233.01.T01:CDS | 45.0% | |
| AACAGTCCATCTTTGGCATC+TGG | - | Chr1:35191772-35191791 | None:intergenic | 45.0% | |
| AGCACAACTTGGAGTGACAA+TGG | - | Chr1:35190559-35190578 | None:intergenic | 45.0% | |
| CGATGATGCTTAGCACAACT+TGG | - | Chr1:35190570-35190589 | None:intergenic | 45.0% | |
| CTGAGAGGACGATAGAAACA+AGG | - | Chr1:35190681-35190700 | None:intergenic | 45.0% | |
| GACGATAGAAACAAGGTGAG+AGG | - | Chr1:35190674-35190693 | None:intergenic | 45.0% | |
| TCGAGAAGCTGTTGTTGTGT+GGG | - | Chr1:35190610-35190629 | None:intergenic | 45.0% | |
| TGCCGAGGGAAACATTTGTT+TGG | - | Chr1:35190486-35190505 | None:intergenic | 45.0% | |
| TGGGCTGAAAAAGAGACGAA+TGG | + | Chr1:35191469-35191488 | MsG0180002233.01.T01:CDS | 45.0% | |
| TTCGTCACATGGCACATACA+TGG | - | Chr1:35191136-35191155 | None:intergenic | 45.0% | |
| ! | GTTGAGATCATCGGTGGATT+TGG | - | Chr1:35190644-35190663 | None:intergenic | 45.0% |
| ! | GTTTTACCTTTCACTCCTGG+AGG | + | Chr1:35191940-35191959 | MsG0180002233.01.T01:CDS | 45.0% |
| !! | AGATGGACTGTTAGCATTGG+TGG | + | Chr1:35191780-35191799 | MsG0180002233.01.T01:CDS | 45.0% |
| !! | GTGGTTTTACCTTTCACTCC+TGG | + | Chr1:35191937-35191956 | MsG0180002233.01.T01:CDS | 45.0% |
| !! | GTTAGCATTGGTGGAGAAGT+TGG | + | Chr1:35191789-35191808 | MsG0180002233.01.T01:CDS | 45.0% |
| !! | TCAACGTAACGGACACTTCT+CGG | + | Chr1:35191254-35191273 | MsG0180002233.01.T01:intron | 45.0% |
| AATAAACTGCATCCCCTCGG+TGG | - | Chr1:35191665-35191684 | None:intergenic | 50.0% | |
| CCATCAACAAAAGAGAGGCG+AGG | - | Chr1:35190724-35190743 | None:intergenic | 50.0% | |
| GAGGTTGTTGAGATCATCGG+TGG | - | Chr1:35190650-35190669 | None:intergenic | 50.0% | |
| GGCGAGGTTGTTGAGATCAT+CGG | - | Chr1:35190653-35190672 | None:intergenic | 50.0% | |
| GTCAAACCTCCAGGAGTGAA+AGG | - | Chr1:35191949-35191968 | None:intergenic | 50.0% | |
| GTCGAGAAGCTGTTGTTGTG+TGG | - | Chr1:35190611-35190630 | None:intergenic | 50.0% | |
| TAGAAACAAGGTGAGAGGCG+AGG | - | Chr1:35190669-35190688 | None:intergenic | 50.0% | |
| ! | CCTCGCCTCTCTTTTGTTGA+TGG | + | Chr1:35190721-35190740 | MsG0180002233.01.T01:CDS | 50.0% |
| !! | GTTGGTGTCATGTCTGACAC+CGG | - | Chr1:35191292-35191311 | None:intergenic | 50.0% |
| !! | TTGGTGTCATGTCTGACACC+GGG | - | Chr1:35191291-35191310 | None:intergenic | 50.0% |
| ACAGATGTTTGGCCATGCCG+AGG | - | Chr1:35190501-35190520 | None:intergenic | 55.0% | |
| ATCCCCTCGGTGGTCATGAA+GGG | - | Chr1:35191655-35191674 | None:intergenic | 55.0% | |
| CAGATGTTTGGCCATGCCGA+GGG | - | Chr1:35190500-35190519 | None:intergenic | 55.0% | |
| GTGCTAAGCATCATCGCTGC+TGG | + | Chr1:35190574-35190593 | MsG0180002233.01.T01:CDS | 55.0% | |
| GTTCCCTTCATGACCACCGA+GGG | + | Chr1:35191649-35191668 | MsG0180002233.01.T01:CDS | 55.0% | |
| TCATGTCTGACACCGGGACA+CGG | - | Chr1:35191285-35191304 | None:intergenic | 55.0% | |
| TTCCCTTCATGACCACCGAG+GGG | + | Chr1:35191650-35191669 | MsG0180002233.01.T01:CDS | 55.0% | |
| ! | CATCGGTGGATTTGGACTCG+AGG | - | Chr1:35190636-35190655 | None:intergenic | 55.0% |
| !! | ATCATCGCTGCTGGCTCTGA+GGG | + | Chr1:35190583-35190602 | MsG0180002233.01.T01:CDS | 55.0% |
| !! | TTCTCGGATTAGCCGTGTCC+CGG | + | Chr1:35191270-35191289 | MsG0180002233.01.T01:intron | 55.0% |
| CAGTGAAAGCGGCGTCTGAG+AGG | - | Chr1:35190696-35190715 | None:intergenic | 60.0% | |
| CATCCCCTCGGTGGTCATGA+AGG | - | Chr1:35191656-35191675 | None:intergenic | 60.0% | |
| GAAGCTGCTGCAGCTAGTGC+TGG | + | Chr1:35192060-35192079 | MsG0180002233.01.T01:CDS | 60.0% | |
| GGTTCCCTTCATGACCACCG+AGG | + | Chr1:35191648-35191667 | MsG0180002233.01.T01:CDS | 60.0% | |
| ! | CTCTCAGACGCCGCTTTCAC+TGG | + | Chr1:35190694-35190713 | MsG0180002233.01.T01:CDS | 60.0% |
| ! | TCAGACGCCGCTTTCACTGG+CGG | + | Chr1:35190697-35190716 | MsG0180002233.01.T01:CDS | 60.0% |
| !! | CATCATCGCTGCTGGCTCTG+AGG | + | Chr1:35190582-35190601 | MsG0180002233.01.T01:CDS | 60.0% |
| ! | CAGACGCCGCTTTCACTGGC+GGG | + | Chr1:35190698-35190717 | MsG0180002233.01.T01:CDS | 65.0% |
| GCGAGGCCCGCCAGTGAAAG+CGG | - | Chr1:35190707-35190726 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 35190433 | 35192209 | 35190433 | ID=MsG0180002233.01;Name=MsG0180002233.01 |
| Chr1 | mRNA | 35190433 | 35192209 | 35190433 | ID=MsG0180002233.01.T01;Parent=MsG0180002233.01;Name=MsG0180002233.01.T01;_AED=0.30;_eAED=0.30;_QI=0|0|0|1|1|1|2|0|393 |
| Chr1 | exon | 35190433 | 35190843 | 35190433 | ID=MsG0180002233.01.T01:exon:2126;Parent=MsG0180002233.01.T01 |
| Chr1 | exon | 35191439 | 35192209 | 35191439 | ID=MsG0180002233.01.T01:exon:2127;Parent=MsG0180002233.01.T01 |
| Chr1 | CDS | 35190433 | 35190843 | 35190433 | ID=MsG0180002233.01.T01:cds;Parent=MsG0180002233.01.T01 |
| Chr1 | CDS | 35191439 | 35192209 | 35191439 | ID=MsG0180002233.01.T01:cds;Parent=MsG0180002233.01.T01 |
| Gene Sequence |
| Protein sequence |
