AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580028235.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028235.01.T01 MTR_5g065980 91.011 178 7 2 1 170 1 177 1.58e-114 322
MsG0580028235.01.T01 MTR_5g065980 90.000 180 7 3 1 170 1 179 1.59e-112 317
MsG0580028235.01.T01 MTR_5g065980 90.476 147 5 2 1 139 1 146 2.97e-89 259
MsG0580028235.01.T01 MTR_1g083950 37.908 153 86 4 10 161 11 155 1.05e-29 107
MsG0580028235.01.T01 MTR_1g054750 35.443 158 96 1 10 161 24 181 2.26e-29 107
MsG0580028235.01.T01 MTR_1g054765 34.756 164 95 3 10 161 5 168 5.88e-29 105
MsG0580028235.01.T01 MTR_1g087200 34.839 155 92 3 10 161 8 156 1.47e-25 96.3
MsG0580028235.01.T01 MTR_1g088640 35.366 164 91 5 2 161 6 158 1.01e-23 91.7
MsG0580028235.01.T01 MTR_1g088640 34.132 167 92 4 2 161 6 161 1.96e-23 90.9
MsG0580028235.01.T01 MTR_7g108830 37.952 166 88 4 1 161 1 156 3.74e-21 85.1
MsG0580028235.01.T01 MTR_8g058620 37.952 166 88 4 1 161 1 156 3.74e-21 85.1
MsG0580028235.01.T01 MTR_5g077130 35.185 162 88 3 9 162 6 158 1.46e-18 78.6
MsG0580028235.01.T01 MTR_5g077130 34.969 163 88 3 9 162 6 159 1.61e-18 78.2
MsG0580028235.01.T01 MTR_4g019800 33.962 159 86 4 12 161 9 157 3.72e-18 77.4
MsG0580028235.01.T01 MTR_7g065770 32.948 173 88 6 1 161 1 157 1.05e-17 76.3
MsG0580028235.01.T01 MTR_4g064933 36.875 160 80 7 12 161 9 157 1.17e-17 75.9
MsG0580028235.01.T01 MTR_2g089350 33.889 180 94 9 4 169 21 189 1.97e-16 73.9
MsG0580028235.01.T01 MTR_6g006190 29.688 128 78 2 40 161 41 162 1.45e-14 68.2
MsG0580028235.01.T01 MTR_1g088620 50.000 64 32 0 98 161 217 280 3.36e-14 68.9
MsG0580028235.01.T01 MTR_6g006190 33.721 86 57 0 76 161 53 138 3.44e-14 66.6
MsG0580028235.01.T01 MTR_2g034200 43.396 53 30 0 109 161 105 157 6.21e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580028235.01.T01 AT1G09740 63.372 172 50 2 1 164 1 167 3.54e-69 207
MsG0580028235.01.T01 AT1G09740 63.372 172 50 2 1 164 1 167 3.54e-69 207
MsG0580028235.01.T01 AT3G11930 38.788 165 95 3 10 168 35 199 3.69e-34 119
MsG0580028235.01.T01 AT3G11930 36.527 167 98 3 10 168 35 201 2.19e-33 117
MsG0580028235.01.T01 AT3G58450 40.000 155 85 4 10 161 32 181 5.04e-33 116
MsG0580028235.01.T01 AT3G58450 38.365 159 89 3 10 161 32 188 1.13e-32 115
MsG0580028235.01.T01 AT3G11930 38.554 166 95 4 10 168 35 200 1.97e-32 115
MsG0580028235.01.T01 AT3G62550 38.217 157 87 4 10 163 9 158 1.17e-28 104
MsG0580028235.01.T01 AT3G11930 33.333 192 95 5 10 168 35 226 1.29e-26 100
MsG0580028235.01.T01 AT2G47710 36.196 163 95 4 1 161 1 156 2.15e-23 90.9
MsG0580028235.01.T01 AT1G68300 34.756 164 95 4 3 163 5 159 1.72e-20 83.2
MsG0580028235.01.T01 AT1G11360 33.140 172 90 8 12 168 42 203 3.60e-17 76.3
MsG0580028235.01.T01 AT1G11360 33.140 172 90 8 12 168 42 203 3.60e-17 76.3
MsG0580028235.01.T01 AT1G11360 33.140 172 90 8 12 168 42 203 3.60e-17 76.3
MsG0580028235.01.T01 AT1G11360 33.140 172 90 8 12 168 42 203 3.60e-17 76.3
MsG0580028235.01.T01 AT3G53990 29.480 173 94 5 1 161 1 157 1.57e-15 70.5
MsG0580028235.01.T01 AT5G14680 30.612 147 86 5 20 161 27 162 4.13e-15 69.7
MsG0580028235.01.T01 AT3G17020 31.579 171 98 4 1 162 1 161 1.13e-14 68.2
MsG0580028235.01.T01 AT3G03270 31.548 168 94 4 5 162 2 158 1.28e-14 67.8
MsG0580028235.01.T01 AT3G01520 28.966 145 87 5 22 161 29 162 1.13e-13 65.9

Find 67 sgRNAs with CRISPR-Local

Find 219 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTTGAATTTATGTTTA+TGG 0.139972 5:-77102294 MsG0580028235.01.T01:three_prime_UTR
TACCTGTGCTATTATATATT+TGG 0.185978 5:-77102439 MsG0580028235.01.T01:three_prime_UTR
AGGAAAGGACGGCGTTAATA+AGG 0.229276 5:-77104182 MsG0580028235.01.T01:CDS
TACTACTACACCGAGGTTTC+CGG 0.232423 5:+77105816 None:intergenic
TAGAATCAGTAATACGTTTC+TGG 0.237367 5:+77105473 None:intergenic
TCGGTGAGTTGCCACATTTC+AGG 0.238300 5:-77102668 MsG0580028235.01.T01:three_prime_UTR
GTGATGGGGTCTCGGGCTTT+TGG 0.264365 5:-77104785 MsG0580028235.01.T01:CDS
TTGGTTGAATTTATGTTTAT+GGG 0.287423 5:-77102293 MsG0580028235.01.T01:three_prime_UTR
ATGTGCTTGTGATGGGGTCT+CGG 0.306029 5:-77104793 MsG0580028235.01.T01:CDS
TTTCCTTTGATGATAATAAC+TGG 0.323710 5:+77104199 None:intergenic
AAAGTTTGTGTTGCCATTGC+TGG 0.333776 5:-77102551 MsG0580028235.01.T01:three_prime_UTR
TGAACTAAAGCTTACATTGT+AGG 0.338700 5:+77105420 None:intergenic
CCACATTTCAGGTGCTGATT+CGG 0.361069 5:-77102657 MsG0580028235.01.T01:three_prime_UTR
TTGTAAGAATCAATGAATAT+TGG 0.369903 5:-77102254 MsG0580028235.01.T01:three_prime_UTR
AGAGCATGAACGCTCTCCGC+TGG 0.407849 5:-77105771 MsG0580028235.01.T01:CDS
CATGCTGATGTGCTTGTGAT+GGG 0.429573 5:-77104800 MsG0580028235.01.T01:CDS
GGATTACTTTAGCTCTGTGT+TGG 0.435091 5:-77102362 MsG0580028235.01.T01:three_prime_UTR
AGAATGTTGTAAACAAATGT+AGG 0.447834 5:-77102383 MsG0580028235.01.T01:three_prime_UTR
GGAAAGGACGGCGTTAATAA+GGG 0.452183 5:-77104181 MsG0580028235.01.T01:CDS
GAAAATTTGTGAAACTGTAC+AGG 0.453240 5:-77104828 MsG0580028235.01.T01:CDS
GTCAGGACTCATGTTGTTGT+TGG 0.460740 5:-77104863 MsG0580028235.01.T01:CDS
ACTAGCGTCGGTGGAATCAT+CGG 0.467478 5:+77105714 None:intergenic
GCATGCTGATGTGCTTGTGA+TGG 0.486669 5:-77104801 MsG0580028235.01.T01:CDS
AGACATACCATGTCGGAATC+CGG 0.487189 5:-77105835 None:intergenic
TTTGGTTGCAGAATGTTCCT+TGG 0.500716 5:-77104259 MsG0580028235.01.T01:intron
TGTGCTTGTGATGGGGTCTC+GGG 0.501916 5:-77104792 MsG0580028235.01.T01:CDS
TCAATTTATGTATAGAACAT+TGG 0.509738 5:-77102312 MsG0580028235.01.T01:three_prime_UTR
CTAGCGTCGGTGGAATCATC+GGG 0.532067 5:+77105715 None:intergenic
TTAAAGCTTAGCTACGGCAC+AGG 0.532626 5:+77102572 None:intergenic
AACTGGACACTCAGAATGGT+GGG 0.536495 5:+77104216 None:intergenic
TTAAGACCGGTGGCGATGGA+CGG 0.538192 5:+77105661 None:intergenic
ATCGCCACCGGTCTTAATCC+CGG 0.539602 5:-77105655 MsG0580028235.01.T01:intron
TCGCTGTTTGTGGACAATAA+TGG 0.543786 5:-77102502 MsG0580028235.01.T01:three_prime_UTR
GGGATTAAGACCGGTGGCGA+TGG 0.547850 5:+77105657 None:intergenic
ATCTATGACAGACTAAGGTC+AGG 0.551554 5:-77104880 MsG0580028235.01.T01:intron
GAGCATGAACGCTCTCCGCT+GGG 0.551654 5:-77105770 MsG0580028235.01.T01:CDS
AAAGCTTTGACATGATCAGT+TGG 0.551687 5:+77102627 None:intergenic
TGTCCAGTTATTATCATCAA+AGG 0.552479 5:-77104202 MsG0580028235.01.T01:CDS
AATCATCGGGTGTAGGTGAT+CGG 0.553013 5:+77105728 None:intergenic
TCACCGCCGTCCATCGCCAC+CGG 0.557458 5:-77105667 MsG0580028235.01.T01:CDS
TGTTGTTGTTGGCGATCCAA+AGG 0.567254 5:-77104852 MsG0580028235.01.T01:CDS
GATGGAACCGGGATTAAGAC+CGG 0.568580 5:+77105648 None:intergenic
TAGCAGTATCTGACCAGCAA+TGG 0.570443 5:+77102538 None:intergenic
TCAATTGCTGCAGCAAACGC+AGG 0.577437 5:+77105502 None:intergenic
AGTTATTATCATCAAAGGAA+AGG 0.590261 5:-77104197 MsG0580028235.01.T01:CDS
TACCAAATATATAATAGCAC+AGG 0.591245 5:+77102437 None:intergenic
GATGAAAGAACTAGCGTCGG+TGG 0.591908 5:+77105705 None:intergenic
GAAGCTATTGCAGTTAAGAG+TGG 0.594611 5:-77102467 MsG0580028235.01.T01:three_prime_UTR
CAAACTTTAAAGCTTAGCTA+CGG 0.601959 5:+77102566 None:intergenic
ACTAAAGCTTACATTGTAGG+TGG 0.603028 5:+77105423 None:intergenic
TAACTGGACACTCAGAATGG+TGG 0.604105 5:+77104215 None:intergenic
TCGGTGGAATCATCGGGTGT+AGG 0.604826 5:+77105721 None:intergenic
ATTATCATCAAAGGAAAGGA+CGG 0.606649 5:-77104193 MsG0580028235.01.T01:CDS
CCGAATCAGCACCTGAAATG+TGG 0.607850 5:+77102657 None:intergenic
GAGGTTTCCGGATTCCGACA+TGG 0.608495 5:+77105828 None:intergenic
AGTAGTTGCTAACACTTCCA+AGG 0.611708 5:+77104242 None:intergenic
ATGCTGATGTGCTTGTGATG+GGG 0.612881 5:-77104799 MsG0580028235.01.T01:CDS
TGGACGGCGGTGATTGAACG+TGG 0.627670 5:+77105677 None:intergenic
TAATAACTGGACACTCAGAA+TGG 0.630290 5:+77104212 None:intergenic
GTAGTAGTAGTAGCAGTAGA+CGG 0.636868 5:-77105802 MsG0580028235.01.T01:CDS
AGACCGGTGGCGATGGACGG+CGG 0.638420 5:+77105664 None:intergenic
ATGTCGGAATCCGGAAACCT+CGG 0.642461 5:-77105826 MsG0580028235.01.T01:CDS
GATGATGAAAGAACTAGCGT+CGG 0.677815 5:+77105702 None:intergenic
GGAACCGGGATTAAGACCGG+TGG 0.712123 5:+77105651 None:intergenic
TGAGATTGTCTAACGCCCAG+CGG 0.722301 5:+77105755 None:intergenic
ACGGCGGTGATTGAACGTGG+AGG 0.796542 5:+77105680 None:intergenic
CTGCTACTACTACTACACCG+AGG 0.818031 5:+77105809 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATTCAACAAATTTAA+GGG + Chr5:77103950-77103969 None:intergenic 10.0%
!! TAAGTTCATATATAAAATAA+AGG - Chr5:77102703-77102722 MsG0580028235.01.T01:intron 10.0%
!! TAATATATTCAACAAATTTA+AGG + Chr5:77103951-77103970 None:intergenic 10.0%
!! TTAAAAATCAATTATATTGT+AGG + Chr5:77104893-77104912 None:intergenic 10.0%
!!! GTTTTATTTTAATTAAAAGT+GGG + Chr5:77104739-77104758 None:intergenic 10.0%
!!! TGTTTTATTTTAATTAAAAG+TGG + Chr5:77104740-77104759 None:intergenic 10.0%
!! TTTAGATTAAATTGATATGT+AGG - Chr5:77104865-77104884 MsG0580028235.01.T01:CDS 15.0%
!!! TTTCATATTTTATTGTTCTA+AGG - Chr5:77103696-77103715 MsG0580028235.01.T01:intron 15.0%
!! AATTAGTCATTTAGTAGTTT+GGG - Chr5:77102986-77103005 MsG0580028235.01.T01:intron 20.0%
!! ACATTATATATATGAACTGA+AGG + Chr5:77103436-77103455 None:intergenic 20.0%
!! ACTTATTATTGTATTATGCA+TGG + Chr5:77104641-77104660 None:intergenic 20.0%
!! ATATATTACCCAAAAAAAGA+AGG - Chr5:77105804-77105823 MsG0580028235.01.T01:CDS 20.0%
!! ATTGGTTGAATTTATGTTTA+TGG - Chr5:77105741-77105760 MsG0580028235.01.T01:CDS 20.0%
!! CAAAATCAAAATATAAGATG+TGG - Chr5:77103634-77103653 MsG0580028235.01.T01:intron 20.0%
!! TAAATTTCTATGTAACTTTC+TGG + Chr5:77103312-77103331 None:intergenic 20.0%
!! TCAATTTATGTATAGAACAT+TGG - Chr5:77105723-77105742 MsG0580028235.01.T01:CDS 20.0%
!! TTATTCCATTAACTTAAAAC+TGG + Chr5:77103087-77103106 None:intergenic 20.0%
!! TTGGTTGAATTTATGTTTAT+GGG - Chr5:77105742-77105761 MsG0580028235.01.T01:CDS 20.0%
!! TTGTAAGAATCAATGAATAT+TGG - Chr5:77105781-77105800 MsG0580028235.01.T01:CDS 20.0%
!!! AAACAAGTTTAGTTTCTTTT+CGG - Chr5:77105348-77105367 MsG0580028235.01.T01:intron 20.0%
!!! AAATTTTTGTACCTCTTAAT+AGG + Chr5:77103277-77103296 None:intergenic 20.0%
!!! AACAATCGACTTAATATTTA+TGG - Chr5:77103336-77103355 MsG0580028235.01.T01:intron 20.0%
!!! AAGAAAGTTTTAACATATTC+CGG + Chr5:77102662-77102681 None:intergenic 20.0%
!!! AATAGTTTAATTTGACTCTT+AGG - Chr5:77104162-77104181 MsG0580028235.01.T01:exon 20.0%
!!! AATTTTTGTACCTCTTAATA+GGG + Chr5:77103276-77103295 None:intergenic 20.0%
!!! CAAAGCATTCATAAAATAAA+TGG + Chr5:77104674-77104693 None:intergenic 20.0%
!!! CTTACTTGTTTATTGTATTT+TGG - Chr5:77102738-77102757 MsG0580028235.01.T01:intron 20.0%
!!! GATAATAATTTTGACTTCTT+AGG - Chr5:77104071-77104090 MsG0580028235.01.T01:intron 20.0%
!!! GTTTTAAGTTAATGGAATAA+TGG - Chr5:77103087-77103106 MsG0580028235.01.T01:intron 20.0%
!!! TCTATTTTTTATCATGCTTT+GGG - Chr5:77102581-77102600 MsG0580028235.01.T01:three_prime_UTR 20.0%
!!! TGAAACATTGATTTTTCATT+AGG - Chr5:77102497-77102516 MsG0580028235.01.T01:three_prime_UTR 20.0%
!!! TTCTATTTTTTATCATGCTT+TGG - Chr5:77102580-77102599 MsG0580028235.01.T01:three_prime_UTR 20.0%
! AAAAAAAGAAGGATGTGATA+TGG - Chr5:77105815-77105834 MsG0580028235.01.T01:CDS 25.0%
! AGAAGTTCAATAGTTACATA+AGG + Chr5:77104117-77104136 None:intergenic 25.0%
! AGAATGTTGTAAACAAATGT+AGG - Chr5:77105652-77105671 MsG0580028235.01.T01:intron 25.0%
! AGTTATTATCATCAAAGGAA+AGG - Chr5:77103838-77103857 MsG0580028235.01.T01:intron 25.0%
! ATTAGTCATTTAGTAGTTTG+GGG - Chr5:77102987-77103006 MsG0580028235.01.T01:intron 25.0%
! GAATTAGTCATTTAGTAGTT+TGG - Chr5:77102985-77103004 MsG0580028235.01.T01:intron 25.0%
! GTTTAAAGATGGAAACTATA+TGG - Chr5:77103727-77103746 MsG0580028235.01.T01:intron 25.0%
! TAAAGCAAGGATAAATTAGT+TGG + Chr5:77105035-77105054 None:intergenic 25.0%
! TACCAAATATATAATAGCAC+AGG + Chr5:77105601-77105620 None:intergenic 25.0%
! TAGTAGTCTACTTAGAATAA+AGG - Chr5:77104195-77104214 MsG0580028235.01.T01:CDS 25.0%
! TAGTGTATATGTATAAAGCA+AGG - Chr5:77105008-77105027 MsG0580028235.01.T01:intron 25.0%
! TCAATGTTTCAGTAAAAACA+TGG + Chr5:77102488-77102507 None:intergenic 25.0%
! TGAACTGAAGGATAAAAAAA+TGG + Chr5:77103424-77103443 None:intergenic 25.0%
! TTATCCTTGCTTTATAATAG+TGG - Chr5:77105041-77105060 MsG0580028235.01.T01:intron 25.0%
! TTTGATATTGCGAATAAAAC+TGG + Chr5:77103667-77103686 None:intergenic 25.0%
!! CTGAAATCTAAATCTAGTTT+AGG - Chr5:77103567-77103586 MsG0580028235.01.T01:intron 25.0%
!! GTAGAAGTTTTTACTACATT+AGG - Chr5:77104311-77104330 MsG0580028235.01.T01:intron 25.0%
!! TACCTGTGCTATTATATATT+TGG - Chr5:77105596-77105615 MsG0580028235.01.T01:intron 25.0%
!! TTTCCTTTGATGATAATAAC+TGG + Chr5:77103839-77103858 None:intergenic 25.0%
!! TTTTGTAATGATGCAAATAG+AGG - Chr5:77104265-77104284 MsG0580028235.01.T01:intron 25.0%
!!! ATATCACATCCTTCTTTTTT+TGG + Chr5:77105816-77105835 None:intergenic 25.0%
!!! TATCACATCCTTCTTTTTTT+GGG + Chr5:77105815-77105834 None:intergenic 25.0%
!!! TGGTATTTTTGAATCTGATT+AGG - Chr5:77103356-77103375 MsG0580028235.01.T01:intron 25.0%
!!! TTTTAATTAAAAGTGGGATG+AGG + Chr5:77104733-77104752 None:intergenic 25.0%
AAGAAAATGATGTACTGAAC+TGG + Chr5:77102426-77102445 None:intergenic 30.0%
AGAAAATGATGTACTGAACT+GGG + Chr5:77102425-77102444 None:intergenic 30.0%
AGGTAGTTATCGAACATTTA+AGG + Chr5:77104097-77104116 None:intergenic 30.0%
AGTCAATTGCAATTCAACTA+CGG + Chr5:77103128-77103147 None:intergenic 30.0%
AGTTCCACTATTATAAAGCA+AGG + Chr5:77105048-77105067 None:intergenic 30.0%
AGTTTGGTATATATAACCGA+AGG - Chr5:77104380-77104399 MsG0580028235.01.T01:intron 30.0%
ATCTGATGTGTGATTAACAA+TGG - Chr5:77105313-77105332 MsG0580028235.01.T01:intron 30.0%
ATTATCATCAAAGGAAAGGA+CGG - Chr5:77103842-77103861 MsG0580028235.01.T01:intron 30.0%
GAAAATTTGTGAAACTGTAC+AGG - Chr5:77103207-77103226 MsG0580028235.01.T01:intron 30.0%
GATAAGCAGAAAATCAAAGA+GGG + Chr5:77104487-77104506 None:intergenic 30.0%
TAATTTGACTCTTAGGAGTT+GGG - Chr5:77104169-77104188 MsG0580028235.01.T01:exon 30.0%
TAGAATCAGTAATACGTTTC+TGG + Chr5:77102565-77102584 None:intergenic 30.0%
TATAGAGTCATAGTTAACGT+TGG - Chr5:77103985-77104004 MsG0580028235.01.T01:intron 30.0%
TCTTTATCTATGACAGACTA+AGG - Chr5:77103150-77103169 MsG0580028235.01.T01:intron 30.0%
TGAACTAAAGCTTACATTGT+AGG + Chr5:77102618-77102637 None:intergenic 30.0%
TGATAAGCAGAAAATCAAAG+AGG + Chr5:77104488-77104507 None:intergenic 30.0%
TGTCCAGTTATTATCATCAA+AGG - Chr5:77103833-77103852 MsG0580028235.01.T01:intron 30.0%
TTAGTCATTTAGTAGTTTGG+GGG - Chr5:77102988-77103007 MsG0580028235.01.T01:intron 30.0%
TTGCGAATAAAACTGGAAAT+CGG + Chr5:77103660-77103679 None:intergenic 30.0%
! AAATTTTCCATTTTCTTCCC+TGG + Chr5:77103493-77103512 None:intergenic 30.0%
! AGGAATCTGAAGTTTAAAGA+TGG - Chr5:77103716-77103735 MsG0580028235.01.T01:intron 30.0%
! CTTATGACTATTTCTTAGGA+CGG - Chr5:77104822-77104841 MsG0580028235.01.T01:CDS 30.0%
! GTACCTTATGACTATTTCTT+AGG - Chr5:77104818-77104837 MsG0580028235.01.T01:CDS 30.0%
! GTTTCACAAATTTTCTCCTT+TGG + Chr5:77103202-77103221 None:intergenic 30.0%
! TTAATTTGACTCTTAGGAGT+TGG - Chr5:77104168-77104187 MsG0580028235.01.T01:exon 30.0%
!! AAAATTCAAAGCATTGTGAG+AGG + Chr5:77103758-77103777 None:intergenic 30.0%
!! CAAACTTTAAAGCTTAGCTA+CGG + Chr5:77105472-77105491 None:intergenic 30.0%
!! CTCACAATGCTTTGAATTTT+TGG - Chr5:77103758-77103777 MsG0580028235.01.T01:intron 30.0%
!!! AGTCTCCAGTTTTAAGTTAA+TGG - Chr5:77103079-77103098 MsG0580028235.01.T01:intron 30.0%
!!! ATGCCATTTTGTGATATGTA+AGG + Chr5:77103383-77103402 None:intergenic 30.0%
ACTAAAGCTTACATTGTAGG+TGG + Chr5:77102615-77102634 None:intergenic 35.0%
AGACTTGCACAAAAAAGTGA+AGG + Chr5:77103064-77103083 None:intergenic 35.0%
AGGTAACACAAGTTACTAGA+TGG - Chr5:77104215-77104234 MsG0580028235.01.T01:CDS 35.0%
AGTAATACCAGGGAAGAAAA+TGG - Chr5:77103483-77103502 MsG0580028235.01.T01:intron 35.0%
AGTTCAATGAGGTTCTTCTA+AGG + Chr5:77104964-77104983 None:intergenic 35.0%
CAGAAAATCAAAGAGGGAAA+GGG + Chr5:77104481-77104500 None:intergenic 35.0%
CGATCTTGATCCAAAAATTG+AGG + Chr5:77105160-77105179 None:intergenic 35.0%
CGTCCTAAGAAATAGTCATA+AGG + Chr5:77104824-77104843 None:intergenic 35.0%
GCAATTCTAACCTTGTAAGT+TGG - Chr5:77103589-77103608 MsG0580028235.01.T01:intron 35.0%
GGACCTTACATATCACAAAA+TGG - Chr5:77103377-77103396 MsG0580028235.01.T01:intron 35.0%
GGATAAAAAAATGGTCCAGA+TGG + Chr5:77103415-77103434 None:intergenic 35.0%
GTTAATCAATGTGATACGCA+TGG - Chr5:77104043-77104062 MsG0580028235.01.T01:intron 35.0%
TAATAACTGGACACTCAGAA+TGG + Chr5:77103826-77103845 None:intergenic 35.0%
TAGTGACAGTGTGTGAAAAT+TGG - Chr5:77102810-77102829 MsG0580028235.01.T01:intron 35.0%
TGCACAAAAAAGTGAAGGTA+GGG + Chr5:77103059-77103078 None:intergenic 35.0%
TGGCATGATAATAAACCATC+TGG - Chr5:77103397-77103416 MsG0580028235.01.T01:intron 35.0%
TGGTATATATAACCGAAGGA+AGG - Chr5:77104384-77104403 MsG0580028235.01.T01:intron 35.0%
TTCTTCCCTGGTATTACTTT+AGG + Chr5:77103481-77103500 None:intergenic 35.0%
TTGCACAAAAAAGTGAAGGT+AGG + Chr5:77103060-77103079 None:intergenic 35.0%
! AAAGCTTTGACATGATCAGT+TGG + Chr5:77105411-77105430 None:intergenic 35.0%
! GAAGAACCTCATTGAACTTT+TGG - Chr5:77104966-77104985 MsG0580028235.01.T01:intron 35.0%
! TTTGGCACATCCTCAATTTT+TGG - Chr5:77105147-77105166 MsG0580028235.01.T01:intron 35.0%
! TTTTACTACATTAGGACACG+TGG - Chr5:77104319-77104338 MsG0580028235.01.T01:intron 35.0%
! TTTTGAGCGATAAGAGTTGT+GGG - Chr5:77103024-77103043 MsG0580028235.01.T01:intron 35.0%
!! ATTTTTGGATCAAGATCGAG+CGG - Chr5:77105162-77105181 MsG0580028235.01.T01:intron 35.0%
!! TTGTTTTTCCTTAGTGTTGC+CGG - Chr5:77102640-77102659 MsG0580028235.01.T01:three_prime_UTR 35.0%
!!! AACCTTGTAAGTTGGTTTTG+TGG - Chr5:77103597-77103616 MsG0580028235.01.T01:intron 35.0%
!!! GTCAAAGCTTTTTCATGCTT+TGG - Chr5:77105420-77105439 MsG0580028235.01.T01:intron 35.0%
AAAGTTTGTGTTGCCATTGC+TGG - Chr5:77105484-77105503 MsG0580028235.01.T01:CDS 40.0%
AACAGGATCTAAGGGAATTG+AGG + Chr5:77104518-77104537 None:intergenic 40.0%
AACATATTCCGGCAACACTA+AGG + Chr5:77102651-77102670 None:intergenic 40.0%
ACACAAGTTACTAGATGGTC+AGG - Chr5:77104220-77104239 MsG0580028235.01.T01:CDS 40.0%
ACACATCTTTCATCGACAGT+TGG - Chr5:77104615-77104634 MsG0580028235.01.T01:intron 40.0%
ACTGGTCCTAAAGTAATACC+AGG - Chr5:77103472-77103491 MsG0580028235.01.T01:intron 40.0%
AGAAAACCCGAGTCTATCAT+TGG - Chr5:77103530-77103549 MsG0580028235.01.T01:intron 40.0%
AGCGGTTTAGACATTAAGCT+CGG - Chr5:77105180-77105199 MsG0580028235.01.T01:intron 40.0%
AGGGATGCAACATAAAAGTG+TGG + Chr5:77103931-77103950 None:intergenic 40.0%
AGTAGTTGCTAACACTTCCA+AGG + Chr5:77103796-77103815 None:intergenic 40.0%
ATCCTAGAGAACAGGATCTA+AGG + Chr5:77104527-77104546 None:intergenic 40.0%
ATCTATGACAGACTAAGGTC+AGG - Chr5:77103155-77103174 MsG0580028235.01.T01:intron 40.0%
ATTCGCGCATTCAGAACATA+AGG + Chr5:77105272-77105291 None:intergenic 40.0%
CACCACAAAACCAACTTACA+AGG + Chr5:77103602-77103621 None:intergenic 40.0%
CACTGACCAAAAGTTCAATG+AGG + Chr5:77104975-77104994 None:intergenic 40.0%
CATTCAGAACATAAGGTCCT+TGG + Chr5:77105265-77105284 None:intergenic 40.0%
CTGGTCCTAAAGTAATACCA+GGG - Chr5:77103473-77103492 MsG0580028235.01.T01:intron 40.0%
GAAGCTATTGCAGTTAAGAG+TGG - Chr5:77105568-77105587 MsG0580028235.01.T01:intron 40.0%
GATGATGAAAGAACTAGCGT+CGG + Chr5:77102336-77102355 None:intergenic 40.0%
GCAGAAAATCAAAGAGGGAA+AGG + Chr5:77104482-77104501 None:intergenic 40.0%
GTAGTAGTAGTAGCAGTAGA+CGG - Chr5:77102233-77102252 MsG0580028235.01.T01:three_prime_UTR 40.0%
GTGTGATTAACAATGGTGTG+AGG - Chr5:77105320-77105339 MsG0580028235.01.T01:intron 40.0%
TAACGTTGGAGTAAGAACTG+AGG - Chr5:77103999-77104018 MsG0580028235.01.T01:intron 40.0%
TAAGGTCCTTGGATCAAGAT+CGG + Chr5:77105254-77105273 None:intergenic 40.0%
TCCTAGAGAACAGGATCTAA+GGG + Chr5:77104526-77104545 None:intergenic 40.0%
TCGCTGTTTGTGGACAATAA+TGG - Chr5:77105533-77105552 MsG0580028235.01.T01:intron 40.0%
TCTCAAGCCAATGATAGACT+CGG + Chr5:77103540-77103559 None:intergenic 40.0%
TGAGGATATACAGTGACTGT+TGG + Chr5:77104715-77104734 None:intergenic 40.0%
TGCACATCAAGACATGAGTT+TGG - Chr5:77104364-77104383 MsG0580028235.01.T01:intron 40.0%
TTAACTTTACTGTCATGCCC+AGG - Chr5:77105089-77105108 MsG0580028235.01.T01:intron 40.0%
TTTGGTTGCAGAATGTTCCT+TGG - Chr5:77103776-77103795 MsG0580028235.01.T01:intron 40.0%
! GTATGAGTTTTCGCTGTTTG+TGG - Chr5:77105523-77105542 MsG0580028235.01.T01:intron 40.0%
! TTGGATTTGCACGTTTTTGC+TGG - Chr5:77105439-77105458 MsG0580028235.01.T01:CDS 40.0%
!! GGATTACTTTAGCTCTGTGT+TGG - Chr5:77105673-77105692 MsG0580028235.01.T01:CDS 40.0%
!! GTTTTGAGCGATAAGAGTTG+TGG - Chr5:77103023-77103042 MsG0580028235.01.T01:intron 40.0%
AACTGGACACTCAGAATGGT+GGG + Chr5:77103822-77103841 None:intergenic 45.0%
AATCATCGGGTGTAGGTGAT+CGG + Chr5:77102310-77102329 None:intergenic 45.0%
AGGAAAGGACGGCGTTAATA+AGG - Chr5:77103853-77103872 MsG0580028235.01.T01:intron 45.0%
ATGCTGATGTGCTTGTGATG+GGG - Chr5:77103236-77103255 MsG0580028235.01.T01:intron 45.0%
ATGTGCCAAATGCGTGAATG+GGG + Chr5:77105137-77105156 None:intergenic 45.0%
ATTTGCCCCATTCACGCATT+TGG - Chr5:77105129-77105148 MsG0580028235.01.T01:intron 45.0%
CAATGTGATACGCATGGAGA+TGG - Chr5:77104049-77104068 MsG0580028235.01.T01:intron 45.0%
CATGCTGATGTGCTTGTGAT+GGG - Chr5:77103235-77103254 MsG0580028235.01.T01:intron 45.0%
CTCAAGCCAATGATAGACTC+GGG + Chr5:77103539-77103558 None:intergenic 45.0%
GATGTGCCAAATGCGTGAAT+GGG + Chr5:77105138-77105157 None:intergenic 45.0%
GCAAGTTCATCCTAGAGAAC+AGG + Chr5:77104535-77104554 None:intergenic 45.0%
GGAAAGGACGGCGTTAATAA+GGG - Chr5:77103854-77103873 MsG0580028235.01.T01:intron 45.0%
GTCAGGACTCATGTTGTTGT+TGG - Chr5:77103172-77103191 MsG0580028235.01.T01:intron 45.0%
GTCATTTAGTAGTTTGGGGG+AGG - Chr5:77102991-77103010 MsG0580028235.01.T01:intron 45.0%
GTTGACAACTAGCTAGACAC+TGG - Chr5:77103454-77103473 MsG0580028235.01.T01:intron 45.0%
TAACTGGACACTCAGAATGG+TGG + Chr5:77103823-77103842 None:intergenic 45.0%
TACTACTACACCGAGGTTTC+CGG + Chr5:77102222-77102241 None:intergenic 45.0%
TAGCAGTATCTGACCAGCAA+TGG + Chr5:77105500-77105519 None:intergenic 45.0%
TCCCTTAGATCCTGTTCTCT+AGG - Chr5:77104522-77104541 MsG0580028235.01.T01:intron 45.0%
TTAAAGCTTAGCTACGGCAC+AGG + Chr5:77105466-77105485 None:intergenic 45.0%
! ATGTTCTGAATGCGCGAATG+CGG - Chr5:77105273-77105292 MsG0580028235.01.T01:intron 45.0%
! TGTTGTTGTTGGCGATCCAA+AGG - Chr5:77103183-77103202 MsG0580028235.01.T01:intron 45.0%
!! CCACATTTCAGGTGCTGATT+CGG - Chr5:77105378-77105397 MsG0580028235.01.T01:intron 45.0%
!! AGATTTAATAAAAAAATTAA+AGG + Chr5:77104923-77104942 None:intergenic 5.0%
AACCGTCCGATCTTGATCCA+AGG - Chr5:77105245-77105264 MsG0580028235.01.T01:intron 50.0%
AATCCCGGTTCCATCCCTTT+CGG - Chr5:77102395-77102414 MsG0580028235.01.T01:three_prime_UTR 50.0%
ATCCTTCTCTCGCCTTCCTT+CGG + Chr5:77104399-77104418 None:intergenic 50.0%
ATGTCGGAATCCGGAAACCT+CGG - Chr5:77102209-77102228 MsG0580028235.01.T01:three_prime_UTR 50.0%
CCGAATCAGCACCTGAAATG+TGG + Chr5:77105381-77105400 None:intergenic 50.0%
CTGCTACTACTACTACACCG+AGG + Chr5:77102229-77102248 None:intergenic 50.0%
GAAGGCGAGAGAAGGATTCA+GGG - Chr5:77104402-77104421 MsG0580028235.01.T01:intron 50.0%
GACGCAAGTGTTACGTGGAT+AGG - Chr5:77104341-77104360 MsG0580028235.01.T01:intron 50.0%
GATGGAACCGGGATTAAGAC+CGG + Chr5:77102390-77102409 None:intergenic 50.0%
GCATGCTGATGTGCTTGTGA+TGG - Chr5:77103234-77103253 MsG0580028235.01.T01:intron 50.0%
GGATGTGCCAAATGCGTGAA+TGG + Chr5:77105139-77105158 None:intergenic 50.0%
GGGGCAAATTTGTGACTGCA+GGG + Chr5:77105118-77105137 None:intergenic 50.0%
GTCCTTGGATCAAGATCGGA+CGG + Chr5:77105250-77105269 None:intergenic 50.0%
TCAATTGCTGCAGCAAACGC+AGG + Chr5:77102536-77102555 None:intergenic 50.0%
TCGGTGAGTTGCCACATTTC+AGG - Chr5:77105367-77105386 MsG0580028235.01.T01:intron 50.0%
TGAGATTGTCTAACGCCCAG+CGG + Chr5:77102283-77102302 None:intergenic 50.0%
TGGGGCAAATTTGTGACTGC+AGG + Chr5:77105119-77105138 None:intergenic 50.0%
TTGTGACTGCAGGGAATCCT+GGG + Chr5:77105109-77105128 None:intergenic 50.0%
TTTGTGACTGCAGGGAATCC+TGG + Chr5:77105110-77105129 None:intergenic 50.0%
! ATGTGCTTGTGATGGGGTCT+CGG - Chr5:77103242-77103261 MsG0580028235.01.T01:intron 50.0%
! GATGAAAGAACTAGCGTCGG+TGG + Chr5:77102333-77102352 None:intergenic 50.0%
! GGGCTTTTGGCCCTATTAAG+AGG - Chr5:77103263-77103282 MsG0580028235.01.T01:intron 50.0%
!! ACTAGCGTCGGTGGAATCAT+CGG + Chr5:77102324-77102343 None:intergenic 50.0%
AACCGAAGGAAGGCGAGAGA+AGG - Chr5:77104394-77104413 MsG0580028235.01.T01:intron 55.0%
ACGTGGACGCAAGTGTTACG+TGG - Chr5:77104336-77104355 MsG0580028235.01.T01:intron 55.0%
ACTGAACTGGGTCCACCGAA+AGG + Chr5:77102413-77102432 None:intergenic 55.0%
ATCGCCACCGGTCTTAATCC+CGG - Chr5:77102380-77102399 MsG0580028235.01.T01:three_prime_UTR 55.0%
CTGAACTGGGTCCACCGAAA+GGG + Chr5:77102412-77102431 None:intergenic 55.0%
GGAAGGCGAGAGAAGGATTC+AGG - Chr5:77104401-77104420 MsG0580028235.01.T01:intron 55.0%
TCCACCGAAAGGGATGGAAC+CGG + Chr5:77102402-77102421 None:intergenic 55.0%
TCGGTGGAATCATCGGGTGT+AGG + Chr5:77102317-77102336 None:intergenic 55.0%
TTAAGACCGGTGGCGATGGA+CGG + Chr5:77102377-77102396 None:intergenic 55.0%
! TGTGCTTGTGATGGGGTCTC+GGG - Chr5:77103243-77103262 MsG0580028235.01.T01:intron 55.0%
!! CTAGCGTCGGTGGAATCATC+GGG + Chr5:77102323-77102342 None:intergenic 55.0%
ACGGCGGTGATTGAACGTGG+AGG + Chr5:77102358-77102377 None:intergenic 60.0%
ACTGGGTCCACCGAAAGGGA+TGG + Chr5:77102408-77102427 None:intergenic 60.0%
AGAGCATGAACGCTCTCCGC+TGG - Chr5:77102264-77102283 MsG0580028235.01.T01:three_prime_UTR 60.0%
CCACCGAAAGGGATGGAACC+GGG + Chr5:77102401-77102420 None:intergenic 60.0%
GAGCATGAACGCTCTCCGCT+GGG - Chr5:77102265-77102284 MsG0580028235.01.T01:three_prime_UTR 60.0%
GGAACCGGGATTAAGACCGG+TGG + Chr5:77102387-77102406 None:intergenic 60.0%
GGGATTAAGACCGGTGGCGA+TGG + Chr5:77102381-77102400 None:intergenic 60.0%
TGGACGGCGGTGATTGAACG+TGG + Chr5:77102361-77102380 None:intergenic 60.0%
!! GTGATGGGGTCTCGGGCTTT+TGG - Chr5:77103250-77103269 MsG0580028235.01.T01:intron 60.0%
CCCGGTTCCATCCCTTTCGG+TGG - Chr5:77102398-77102417 MsG0580028235.01.T01:three_prime_UTR 65.0%
AGACCGGTGGCGATGGACGG+CGG + Chr5:77102374-77102393 None:intergenic 70.0%
TCACCGCCGTCCATCGCCAC+CGG - Chr5:77102368-77102387 MsG0580028235.01.T01:three_prime_UTR 70.0%
Chromosome Type Strat End Strand Name
Chr5 gene 77102209 77105848 77102209 ID=MsG0580028235.01;Name=MsG0580028235.01
Chr5 mRNA 77102209 77105848 77102209 ID=MsG0580028235.01.T01;Parent=MsG0580028235.01;Name=MsG0580028235.01.T01;_AED=0.36;_eAED=0.36;_QI=0|0.75|0.8|1|0.75|0.6|5|523|170
Chr5 exon 77102209 77102697 77102209 ID=MsG0580028235.01.T01:exon:24121;Parent=MsG0580028235.01.T01
Chr5 exon 77104140 77104270 77104140 ID=MsG0580028235.01.T01:exon:24120;Parent=MsG0580028235.01.T01
Chr5 exon 77104773 77104891 77104773 ID=MsG0580028235.01.T01:exon:24119;Parent=MsG0580028235.01.T01
Chr5 exon 77105435 77105538 77105435 ID=MsG0580028235.01.T01:exon:24118;Parent=MsG0580028235.01.T01
Chr5 exon 77105656 77105848 77105656 ID=MsG0580028235.01.T01:exon:24117;Parent=MsG0580028235.01.T01
Chr5 CDS 77105656 77105848 77105656 ID=MsG0580028235.01.T01:cds;Parent=MsG0580028235.01.T01
Chr5 CDS 77105435 77105538 77105435 ID=MsG0580028235.01.T01:cds;Parent=MsG0580028235.01.T01
Chr5 CDS 77104773 77104891 77104773 ID=MsG0580028235.01.T01:cds;Parent=MsG0580028235.01.T01
Chr5 CDS 77104174 77104270 77104174 ID=MsG0580028235.01.T01:cds;Parent=MsG0580028235.01.T01
Chr5 three_prime_UTR 77104140 77104173 77104140 ID=MsG0580028235.01.T01:three_prime_utr;Parent=MsG0580028235.01.T01
Chr5 three_prime_UTR 77102209 77102697 77102209 ID=MsG0580028235.01.T01:three_prime_utr;Parent=MsG0580028235.01.T01
Gene Sequence

>MsG0580028235.01.T01

ATGTCGGAATCCGGAAACCTCGGTGTAGTAGTAGTAGCAGTAGACGGAAGCGAAGAGAGCATGAACGCTCTCCGCTGGGCGTTAGACAATCTCAAGCTCCGATCACCTACACCCGATGATTCCACCGACGCTAGTTCTTTCATCATCCTCCACGTTCAATCACCGCCGTCCATCGCCACCGGTCTTAATCCCGGTGATCTTGAAGTTCCTGCGTTTGCTGCAGCAATTGAAGCTCACCAGAAACGTATTACTGATTCTATTTTTTATCATGCTTTGGGAATTTGCTCCACCTACAATACTAAGGTCAGGACTCATGTTGTTGTTGGCGATCCAAAGGAGAAAATTTGTGAAACTGTACAGGATTTGCATGCTGATGTGCTTGTGATGGGGTCTCGGGCTTTTGGCCCTATTAAGAGAATGTTCCTTGGAAGTGTTAGCAACTACTGCGCCCACCATTCTGAGTGTCCAGTTATTATCATCAAAGGAAAGGACGGCGTTAATAAGGGAAACTAA
Protein sequence

>MsG0580028235.01.T01

MSESGNLGVVVVAVDGSEESMNALRWALDNLKLRSPTPDDSTDASSFIILHVQSPPSIATGLNPGDLEVPAFAAAIEAHQKRITDSIFYHALGICSTYNTKVRTHVVVGDPKEKICETVQDLHADVLVMGSRAFGPIKRMFLGSVSNYCAHHSECPVIIIKGKDGVNKGN*