AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880044001.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044001.01.T01 MTR_8g046000 94.954 218 11 0 1 218 19 236 4.43e-159 439
MsG0880044001.01.T01 MTR_8g045890 66.049 162 55 0 57 218 284 445 7.63e-76 235
MsG0880044001.01.T01 MTR_8g045890 66.049 162 55 0 57 218 325 486 3.04e-75 235
MsG0880044001.01.T01 MTR_8g045890 66.049 162 55 0 57 218 362 523 5.51e-75 235
MsG0880044001.01.T01 MTR_8g445140 50.000 244 70 4 13 204 59 302 8.50e-75 228
MsG0880044001.01.T01 MTR_8g045880 61.458 192 66 2 35 218 137 328 4.18e-74 227
MsG0880044001.01.T01 MTR_8g445170 61.364 176 63 2 43 218 227 397 4.96e-74 229
MsG0880044001.01.T01 MTR_8g045880 61.458 192 66 2 35 218 172 363 1.33e-73 227
MsG0880044001.01.T01 MTR_8g045880 61.458 192 66 2 35 218 174 365 1.97e-73 227
MsG0880044001.01.T01 MTR_1g045860 54.762 210 82 5 13 218 69 269 1.84e-72 221
MsG0880044001.01.T01 MTR_2g081770 48.980 196 92 4 13 204 79 270 1.84e-60 191
MsG0880044001.01.T01 MTR_2g081610 46.429 196 96 3 11 204 71 259 2.08e-60 191
MsG0880044001.01.T01 MTR_2g081590 46.907 194 94 3 13 204 73 259 3.79e-58 185
MsG0880044001.01.T01 MTR_8g044290 59.864 147 59 0 35 181 197 343 4.44e-55 182
MsG0880044001.01.T01 MTR_3g109490 34.234 222 121 9 7 204 101 321 2.41e-34 125
MsG0880044001.01.T01 MTR_1g008460 34.272 213 114 7 12 203 88 295 4.35e-33 121
MsG0880044001.01.T01 MTR_1g008470 36.916 214 110 9 12 203 88 298 1.42e-32 119
MsG0880044001.01.T01 MTR_1g008590 36.697 218 106 9 12 203 112 323 1.20e-31 117
MsG0880044001.01.T01 MTR_1g008560 36.574 216 112 8 12 203 114 328 1.67e-31 117
MsG0880044001.01.T01 MTR_1g008530 35.648 216 114 8 12 203 8 222 2.39e-31 114
MsG0880044001.01.T01 MTR_0312s0030 36.697 218 106 9 12 203 112 323 2.83e-31 117
MsG0880044001.01.T01 MTR_1g007520 36.697 218 106 9 12 203 112 323 2.83e-31 117
MsG0880044001.01.T01 MTR_1g008580 42.336 137 72 4 74 203 34 170 2.99e-31 112
MsG0880044001.01.T01 MTR_1g008500 36.111 216 112 8 12 203 170 383 2.85e-30 115
MsG0880044001.01.T01 MTR_1g008510 36.574 216 108 8 12 203 114 324 3.67e-30 114
MsG0880044001.01.T01 MTR_1g008450 40.000 135 76 4 74 203 177 311 1.14e-25 102
MsG0880044001.01.T01 MTR_3g116270 30.734 218 125 7 13 206 292 507 3.06e-22 94.4
MsG0880044001.01.T01 MTR_3g107830 38.514 148 81 5 67 204 108 255 9.84e-22 90.5
MsG0880044001.01.T01 MTR_3g116320 31.193 218 124 7 13 206 474 689 1.27e-21 92.8
MsG0880044001.01.T01 MTR_4g069680 37.838 148 82 6 67 204 139 286 5.36e-20 86.3
MsG0880044001.01.T01 MTR_3g116410 37.838 148 82 5 67 204 128 275 1.64e-19 84.7
MsG0880044001.01.T01 MTR_4g069570 36.364 154 84 6 65 205 184 336 3.98e-18 81.6
MsG0880044001.01.T01 MTR_3g116380 31.169 231 125 10 4 205 78 303 6.41e-18 80.9
MsG0880044001.01.T01 MTR_4g069550 30.065 153 91 7 67 205 313 463 3.27e-12 65.5
MsG0880044001.01.T01 MTR_5g033350 30.968 155 96 5 57 202 46 198 4.86e-12 63.2
MsG0880044001.01.T01 MTR_4g133840 32.298 161 97 7 52 202 28 186 7.53e-12 62.4
MsG0880044001.01.T01 MTR_5g033340 30.968 155 96 5 57 202 225 377 1.76e-11 63.2
MsG0880044001.01.T01 MTR_4g068420 30.597 134 74 6 78 205 1 121 7.78e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880044001.01.T01 AT5G25610 37.054 224 111 9 7 203 170 390 2.57e-31 118
MsG0880044001.01.T01 AT1G70370 32.192 146 87 5 67 202 479 622 7.71e-13 67.4
MsG0880044001.01.T01 AT1G70370 32.192 146 87 5 67 202 479 622 7.71e-13 67.4

Find 48 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAACTTCGTCAAGCAAATT+TGG 0.185829 8:+34137041 None:intergenic
GTTGAATACTGGAAAGCTTT+TGG 0.241403 8:+34137480 None:intergenic
ACGAAAATTAAGTCATATTC+AGG 0.260765 8:-34137278 MsG0880044001.01.T01:CDS
GGCAAATTCCTTCATTGTTT+CGG 0.292399 8:+34137316 None:intergenic
TGTTCGGAATGGTTGAATAC+TGG 0.348637 8:+34137469 None:intergenic
AACTTACATGGTTCCATTGT+TGG 0.360469 8:-34137126 MsG0880044001.01.T01:CDS
GATTCACTTGCAACAGGAAT+AGG 0.379182 8:+34136954 None:intergenic
CAGCCTTGTTACCAACAAAA+TGG 0.391110 8:+34136988 None:intergenic
TTTAATAATGCAGACATTGA+AGG 0.399608 8:-34137557 MsG0880044001.01.T01:CDS
ATTCAACCATTCCGAACAAT+AGG 0.412309 8:-34137464 MsG0880044001.01.T01:CDS
ATATCCAAATATCATTTAGA+TGG 0.413763 8:+34136914 None:intergenic
TGGCAGACAGGGATAGTTCC+AGG 0.415844 8:+34137008 None:intergenic
TTACTTCTTCCACTACATAT+TGG 0.433811 8:+34137234 None:intergenic
GTTCCATTGTTGGCCTCTGA+TGG 0.457551 8:-34137116 MsG0880044001.01.T01:CDS
TATAGTCTTGACGAGATTTG+TGG 0.471610 8:-34137383 MsG0880044001.01.T01:CDS
AATATGTAGTGGAAGAAGTA+AGG 0.480623 8:-34137232 MsG0880044001.01.T01:CDS
TCATCAGATTCACTTGCAAC+AGG 0.481970 8:+34136948 None:intergenic
AACTTGTCTTCTCCGATAGC+AGG 0.508034 8:+34137353 None:intergenic
TTGGTAACAAGGCTGTTGCT+TGG 0.508285 8:-34136980 MsG0880044001.01.T01:CDS
TCGGCAACCATCCTATTGTT+CGG 0.521145 8:+34137453 None:intergenic
AACCATCCTATTGTTCGGAA+TGG 0.524994 8:+34137458 None:intergenic
GATTCATACCTCTTGTGTCA+TGG 0.531056 8:+34137063 None:intergenic
AACATCCTCCGAAACAATGA+AGG 0.533172 8:-34137324 MsG0880044001.01.T01:CDS
GAATTTGCCATTTCAAAACT+CGG 0.533199 8:-34137302 MsG0880044001.01.T01:CDS
TTGGAAAACGACCTATACCC+TGG 0.535252 8:-34137521 MsG0880044001.01.T01:CDS
AATTTGCCATTTCAAAACTC+GGG 0.540236 8:-34137301 MsG0880044001.01.T01:CDS
GAAGTTCTCAAAGTCAAGCC+TGG 0.543141 8:-34137026 MsG0880044001.01.T01:CDS
TGTGGAGGTCCACCTGCTAT+CGG 0.544677 8:-34137365 MsG0880044001.01.T01:CDS
TTGTTGATGCATTCACTTGG+TGG 0.566717 8:+34137147 None:intergenic
AAATTCCTTCATTGTTTCGG+AGG 0.567274 8:+34137319 None:intergenic
GAATGCATCAACAACTTACA+TGG 0.567371 8:-34137138 MsG0880044001.01.T01:CDS
TTACCAACAAAATGGCAGAC+AGG 0.571358 8:+34136996 None:intergenic
TGGAACTAAAGTTAATGCAT+TGG 0.576995 8:-34137096 MsG0880044001.01.T01:CDS
ATTAACTTTAGTTCCATCAG+AGG 0.588740 8:+34137103 None:intergenic
GTTCCATCAGAGGCCAACAA+TGG 0.589021 8:+34137113 None:intergenic
TTCTCCACTACATTTCCAGA+CGG 0.593237 8:+34137413 None:intergenic
AACCATTCCGAACAATAGGA+TGG 0.593760 8:-34137460 MsG0880044001.01.T01:CDS
AAATCAAGACCAATATGTAG+TGG 0.595851 8:-34137243 MsG0880044001.01.T01:CDS
TACCAACAAAATGGCAGACA+GGG 0.611221 8:+34136997 None:intergenic
GTAAGGAAAATTGCAGACAA+AGG 0.616323 8:-34137215 MsG0880044001.01.T01:CDS
AGTCTTGACGAGATTTGTGG+AGG 0.619391 8:-34137380 MsG0880044001.01.T01:CDS
TTGTCTTCTCCGATAGCAGG+TGG 0.637131 8:+34137356 None:intergenic
TCAACCGTCTGGAAATGTAG+TGG 0.641252 8:-34137417 MsG0880044001.01.T01:CDS
TCATTTAGATGGCACAAGGA+CGG 0.647569 8:+34136925 None:intergenic
GCTTGTCACCATGACACAAG+AGG 0.650773 8:-34137071 MsG0880044001.01.T01:CDS
TACCCTGGCAAGAAAATGAG+CGG 0.655096 8:-34137506 MsG0880044001.01.T01:CDS
AAGTTGTTGATGCATTCACT+TGG 0.662304 8:+34137144 None:intergenic
AATATCATTTAGATGGCACA+AGG 0.695284 8:+34136921 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACGAAAATTAAGTCATATTC+AGG - Chr8:34137214-34137233 MsG0880044001.01.T01:CDS 25.0%
! TTTAATAATGCAGACATTGA+AGG - Chr8:34136935-34136954 MsG0880044001.01.T01:CDS 25.0%
AAATCAAGACCAATATGTAG+TGG - Chr8:34137249-34137268 MsG0880044001.01.T01:CDS 30.0%
TGGAACTAAAGTTAATGCAT+TGG - Chr8:34137396-34137415 MsG0880044001.01.T01:CDS 30.0%
TTACTTCTTCCACTACATAT+TGG + Chr8:34137261-34137280 None:intergenic 30.0%
! AATATGTAGTGGAAGAAGTA+AGG - Chr8:34137260-34137279 MsG0880044001.01.T01:CDS 30.0%
! AATTTGCCATTTCAAAACTC+GGG - Chr8:34137191-34137210 MsG0880044001.01.T01:CDS 30.0%
! ATTAACTTTAGTTCCATCAG+AGG + Chr8:34137392-34137411 None:intergenic 30.0%
! GAATTTGCCATTTCAAAACT+CGG - Chr8:34137190-34137209 MsG0880044001.01.T01:CDS 30.0%
! TGAAGGCAAATTACTCTTTT+TGG - Chr8:34136952-34136971 MsG0880044001.01.T01:CDS 30.0%
AAATTCCTTCATTGTTTCGG+AGG + Chr8:34137176-34137195 None:intergenic 35.0%
AACTTACATGGTTCCATTGT+TGG - Chr8:34137366-34137385 MsG0880044001.01.T01:CDS 35.0%
AAGTTGTTGATGCATTCACT+TGG + Chr8:34137351-34137370 None:intergenic 35.0%
AGAACTTCGTCAAGCAAATT+TGG + Chr8:34137454-34137473 None:intergenic 35.0%
ATTCAACCATTCCGAACAAT+AGG - Chr8:34137028-34137047 MsG0880044001.01.T01:CDS 35.0%
GAATGCATCAACAACTTACA+TGG - Chr8:34137354-34137373 MsG0880044001.01.T01:CDS 35.0%
GGCAAATTCCTTCATTGTTT+CGG + Chr8:34137179-34137198 None:intergenic 35.0%
GTAAGGAAAATTGCAGACAA+AGG - Chr8:34137277-34137296 MsG0880044001.01.T01:CDS 35.0%
! GTTGAATACTGGAAAGCTTT+TGG + Chr8:34137015-34137034 None:intergenic 35.0%
! TATAGTCTTGACGAGATTTG+TGG - Chr8:34137109-34137128 MsG0880044001.01.T01:CDS 35.0%
AACATCCTCCGAAACAATGA+AGG - Chr8:34137168-34137187 MsG0880044001.01.T01:CDS 40.0%
AACCATCCTATTGTTCGGAA+TGG + Chr8:34137037-34137056 None:intergenic 40.0%
AACCATTCCGAACAATAGGA+TGG - Chr8:34137032-34137051 MsG0880044001.01.T01:CDS 40.0%
GATTCACTTGCAACAGGAAT+AGG + Chr8:34137541-34137560 None:intergenic 40.0%
GATTCATACCTCTTGTGTCA+TGG + Chr8:34137432-34137451 None:intergenic 40.0%
TACCAACAAAATGGCAGACA+GGG + Chr8:34137498-34137517 None:intergenic 40.0%
TCATCAGATTCACTTGCAAC+AGG + Chr8:34137547-34137566 None:intergenic 40.0%
TGTTCGGAATGGTTGAATAC+TGG + Chr8:34137026-34137045 None:intergenic 40.0%
TTACCAACAAAATGGCAGAC+AGG + Chr8:34137499-34137518 None:intergenic 40.0%
TTCTCCACTACATTTCCAGA+CGG + Chr8:34137082-34137101 None:intergenic 40.0%
TTGTTGATGCATTCACTTGG+TGG + Chr8:34137348-34137367 None:intergenic 40.0%
! TCATTTTCTTGCCAGGGTAT+AGG + Chr8:34136985-34137004 None:intergenic 40.0%
!! CAGCCTTGTTACCAACAAAA+TGG + Chr8:34137507-34137526 None:intergenic 40.0%
!!! CTGCCATTTTGTTGGTAACA+AGG - Chr8:34137501-34137520 MsG0880044001.01.T01:CDS 40.0%
!!! GGTTGAGTAGCTTTTTCGAT+CGG + Chr8:34137061-34137080 None:intergenic 40.0%
!!! TTTCGTCCCGAGTTTTGAAA+TGG + Chr8:34137200-34137219 None:intergenic 40.0%
AACTTGTCTTCTCCGATAGC+AGG + Chr8:34137142-34137161 None:intergenic 45.0%
AGTCTTGACGAGATTTGTGG+AGG - Chr8:34137112-34137131 MsG0880044001.01.T01:CDS 45.0%
GAAAAAGCTACTCAACCGTC+TGG - Chr8:34137064-34137083 MsG0880044001.01.T01:CDS 45.0%
GAAGTTCTCAAAGTCAAGCC+TGG - Chr8:34137466-34137485 MsG0880044001.01.T01:CDS 45.0%
TACCCTGGCAAGAAAATGAG+CGG - Chr8:34136986-34137005 MsG0880044001.01.T01:CDS 45.0%
TCAACCGTCTGGAAATGTAG+TGG - Chr8:34137075-34137094 MsG0880044001.01.T01:CDS 45.0%
TCGGCAACCATCCTATTGTT+CGG + Chr8:34137042-34137061 None:intergenic 45.0%
TTGGAAAACGACCTATACCC+TGG - Chr8:34136971-34136990 MsG0880044001.01.T01:CDS 45.0%
! TTGGTAACAAGGCTGTTGCT+TGG - Chr8:34137512-34137531 MsG0880044001.01.T01:CDS 45.0%
!!! ATCCCTGTCTGCCATTTTGT+TGG - Chr8:34137493-34137512 MsG0880044001.01.T01:CDS 45.0%
GCTTGTCACCATGACACAAG+AGG - Chr8:34137421-34137440 MsG0880044001.01.T01:CDS 50.0%
GTTCCATCAGAGGCCAACAA+TGG + Chr8:34137382-34137401 None:intergenic 50.0%
TTGTCTTCTCCGATAGCAGG+TGG + Chr8:34137139-34137158 None:intergenic 50.0%
! AGACCGCTCATTTTCTTGCC+AGG + Chr8:34136992-34137011 None:intergenic 50.0%
! GACCGCTCATTTTCTTGCCA+GGG + Chr8:34136991-34137010 None:intergenic 50.0%
! GTTCCATTGTTGGCCTCTGA+TGG - Chr8:34137376-34137395 MsG0880044001.01.T01:CDS 50.0%
TGGCAGACAGGGATAGTTCC+AGG + Chr8:34137487-34137506 None:intergenic 55.0%
TGTGGAGGTCCACCTGCTAT+CGG - Chr8:34137127-34137146 MsG0880044001.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 34136929 34137585 34136929 ID=MsG0880044001.01;Name=MsG0880044001.01
Chr8 mRNA 34136929 34137585 34136929 ID=MsG0880044001.01.T01;Parent=MsG0880044001.01;Name=MsG0880044001.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|218
Chr8 exon 34136929 34137585 34136929 ID=MsG0880044001.01.T01:exon:8168;Parent=MsG0880044001.01.T01
Chr8 CDS 34136929 34137585 34136929 ID=MsG0880044001.01.T01:cds;Parent=MsG0880044001.01.T01
Gene Sequence

>MsG0880044001.01.T01

ATGATTTTTAATAATGCAGACATTGAAGGCAAATTACTCTTTTTGGAAAACGACCTATACCCTGGCAAGAAAATGAGCGGTCTCCAAAAGCTTTCCAGTATTCAACCATTCCGAACAATAGGATGGTTGCCGATCGAAAAAGCTACTCAACCGTCTGGAAATGTAGTGGAGAAAGAGAGTTATAGTCTTGACGAGATTTGTGGAGGTCCACCTGCTATCGGAGAAGACAAGTTTTGCGCAACATCCTCCGAAACAATGAAGGAATTTGCCATTTCAAAACTCGGGACGAAAATTAAGTCATATTCAGGTTACTTTGCTAAAAATCAAGACCAATATGTAGTGGAAGAAGTAAGGAAAATTGCAGACAAAGGTGTAATGTGTCATCGAATGAATTTCGAGAAAGTTGTATTTTATTGCCACCAAGTGAATGCATCAACAACTTACATGGTTCCATTGTTGGCCTCTGATGGAACTAAAGTTAATGCATTGGCTGCTTGTCACCATGACACAAGAGGTATGAATCCAAATTTGCTTGACGAAGTTCTCAAAGTCAAGCCTGGAACTATCCCTGTCTGCCATTTTGTTGGTAACAAGGCTGTTGCTTGGCTACCTATTCCTGTTGCAAGTGAATCTGATGACCGTCCTTGTGCCATCTAA

Protein sequence

>MsG0880044001.01.T01

MIFNNADIEGKLLFLENDLYPGKKMSGLQKLSSIQPFRTIGWLPIEKATQPSGNVVEKESYSLDEICGGPPAIGEDKFCATSSETMKEFAISKLGTKIKSYSGYFAKNQDQYVVEEVRKIADKGVMCHRMNFEKVVFYCHQVNASTTYMVPLLASDGTKVNALAACHHDTRGMNPNLLDEVLKVKPGTIPVCHFVGNKAVAWLPIPVASESDDRPCAI*